LOCUS       DAJSVP010000001        87343 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSVP010000001 DAJSVP010000000
VERSION     DAJSVP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.001"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ON449_000001"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ON449_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3932624.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ON449_000002"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ON449_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3932625.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ON449_000003"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ON449_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3932626.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ON449_000004"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ON449_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3932627.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ON449_000005"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ON449_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3932628.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ON449_000006"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ON449_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3932629.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ON449_000007"
     CDS             complement(8053..8523)
                     /locus_tag="ON449_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3932630.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ON449_000008"
     CDS             complement(8569..8994)
                     /locus_tag="ON449_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3932631.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ON449_000009"
     CDS             complement(9061..9330)
                     /locus_tag="ON449_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3932632.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ON449_000010"
     CDS             complement(9341..10936)
                     /locus_tag="ON449_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3932633.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ON449_000011"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ON449_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3932634.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ON449_000012"
     CDS             complement(14489..15049)
                     /locus_tag="ON449_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3932635.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ON449_000013"
     CDS             15402..16376
                     /locus_tag="ON449_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3932636.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ON449_000014"
     CDS             16546..17946
                     /locus_tag="ON449_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3932637.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ON449_000015"
     CDS             complement(18024..18746)
                     /locus_tag="ON449_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3932638.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ON449_000016"
     CDS             complement(18825..19331)
                     /locus_tag="ON449_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3932639.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ON449_000017"
     CDS             19454..21253
                     /locus_tag="ON449_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3932640.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ON449_000018"
     CDS             complement(21321..22895)
                     /locus_tag="ON449_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3932641.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ON449_000019"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ON449_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3932642.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ON449_000020"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ON449_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3932643.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ON449_000021"
     CDS             complement(24672..25310)
                     /locus_tag="ON449_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3932644.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ON449_000022"
     CDS             complement(25311..25961)
                     /locus_tag="ON449_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3932645.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ON449_000023"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ON449_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3932646.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ON449_000024"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ON449_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3932647.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ON449_000025"
     CDS             complement(29104..29844)
                     /locus_tag="ON449_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3932648.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ON449_000026"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ON449_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3932649.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ON449_000027"
     CDS             complement(31215..32153)
                     /locus_tag="ON449_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3932650.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ON449_000028"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ON449_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3932651.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ON449_000029"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ON449_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3932652.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ON449_000030"
     CDS             complement(35313..35618)
                     /locus_tag="ON449_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3932653.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ON449_000031"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ON449_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3932654.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ON449_000032"
     CDS             complement(36724..37008)
                     /locus_tag="ON449_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932655.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ON449_000033"
     CDS             complement(37026..37916)
                     /locus_tag="ON449_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3932656.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ON449_000034"
     CDS             complement(37936..38154)
                     /locus_tag="ON449_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3932657.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ON449_000035"
     CDS             complement(38124..39824)
                     /locus_tag="ON449_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3932658.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ON449_000036"
     CDS             40091..40864
                     /locus_tag="ON449_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932659.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ON449_000037"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ON449_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3932660.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ON449_000038"
     CDS             complement(41688..42659)
                     /locus_tag="ON449_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3932661.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ON449_000039"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ON449_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3932662.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ON449_000040"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ON449_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3932663.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ON449_000041"
     CDS             complement(45614..46438)
                     /locus_tag="ON449_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3932664.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ON449_000042"
     CDS             complement(46411..47091)
                     /locus_tag="ON449_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3932665.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ON449_000043"
     CDS             complement(47114..48052)
                     /locus_tag="ON449_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3932666.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ON449_000044"
     CDS             complement(48297..49160)
                     /locus_tag="ON449_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3932667.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ON449_000045"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ON449_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3932668.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ON449_000046"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ON449_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3932669.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ON449_000047"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ON449_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3932670.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ON449_000048"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ON449_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3932671.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ON449_000049"
     CDS             complement(56026..57267)
                     /locus_tag="ON449_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3932672.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ON449_000050"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ON449_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3932673.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ON449_000051"
     CDS             complement(58294..59217)
                     /locus_tag="ON449_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3932674.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ON449_000052"
     CDS             complement(59956..61242)
                     /locus_tag="ON449_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3932675.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ON449_000053"
     CDS             complement(61235..62503)
                     /locus_tag="ON449_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3932676.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="ON449_000054"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="ON449_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3932677.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="ON449_000055"
     CDS             complement(63275..63721)
                     /locus_tag="ON449_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3932678.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="ON449_000056"
     CDS             complement(63814..64059)
                     /locus_tag="ON449_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3932679.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="ON449_000057"
     CDS             complement(64174..64752)
                     /locus_tag="ON449_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3932680.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="ON449_000058"
     CDS             complement(64829..65359)
                     /locus_tag="ON449_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3932681.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="ON449_000059"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="ON449_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3932682.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="ON449_000060"
     CDS             complement(66216..66611)
                     /locus_tag="ON449_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3932683.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="ON449_000061"
     CDS             complement(66608..68020)
                     /locus_tag="ON449_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3932684.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="ON449_000062"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="ON449_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3932685.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68639..68878)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68875..70335)
                     /locus_tag="ON449_000063"
     CDS             complement(68875..70335)
                     /locus_tag="ON449_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3932686.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="ON449_000064"
     CDS             complement(70505..71662)
                     /locus_tag="ON449_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3932687.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="ON449_000065"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="ON449_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3932688.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="ON449_000066"
     CDS             complement(73129..73656)
                     /locus_tag="ON449_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3932689.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="ON449_000067"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="ON449_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3932690.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="ON449_000068"
     CDS             74689..75936
                     /locus_tag="ON449_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3932691.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="ON449_000069"
     CDS             76012..76356
                     /locus_tag="ON449_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932692.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="ON449_000070"
     CDS             complement(76473..76799)
                     /locus_tag="ON449_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932693.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="ON449_000071"
     CDS             complement(77414..78616)
                     /locus_tag="ON449_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3932694.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="ON449_000072"
     CDS             78733..79248
                     /locus_tag="ON449_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3932695.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="ON449_000073"
     CDS             complement(79447..79893)
                     /locus_tag="ON449_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3932696.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr(C)"
                     /locus_tag="ON449_000074"
     CDS             complement(79940..81418)
                     /gene="msr(C)"
                     /locus_tag="ON449_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3932697.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="ON449_000075"
     CDS             complement(81772..82401)
                     /locus_tag="ON449_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3932698.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="ON449_000076"
     CDS             complement(82385..82849)
                     /locus_tag="ON449_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3932699.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="ON449_000077"
     CDS             complement(83336..84604)
                     /locus_tag="ON449_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3932700.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="ON449_000078"
     CDS             complement(84835..85572)
                     /locus_tag="ON449_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3932701.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86208)
                     /locus_tag="ON449_000079"
     CDS             complement(85639..86208)
                     /locus_tag="ON449_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3932702.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86336..87208
                     /locus_tag="ON449_000080"
     CDS             86336..87208
                     /locus_tag="ON449_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3932703.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        24258 a        18881 c        15678 g        28526 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    85741 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    85801 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    85861 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    85921 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    85981 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86041 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86101 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86161 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86221 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86281 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86341 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86401 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    86461 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    86521 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    86581 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    86641 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    86701 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    86761 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    86821 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    86881 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    86941 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87001 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87061 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87121 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87181 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87241 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87301 tccttccttt tatttctgat aattccagta aattactata att
//
LOCUS       DAJSVP010000002        77037 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSVP010000002 DAJSVP010000000
VERSION     DAJSVP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.002"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(3..653)
                     /locus_tag="ON449_000081"
     CDS             complement(3..653)
                     /locus_tag="ON449_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932704.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(753..1262)
                     /locus_tag="ON449_000082"
     CDS             complement(753..1262)
                     /locus_tag="ON449_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3932705.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1347..1826)
                     /gene="rlmH"
                     /locus_tag="ON449_000083"
     CDS             complement(1347..1826)
                     /gene="rlmH"
                     /locus_tag="ON449_000083"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3932706.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2254..3543
                     /locus_tag="ON449_000084"
     CDS             2254..3543
                     /locus_tag="ON449_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3932707.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3582..4187)
                     /locus_tag="ON449_000085"
     CDS             complement(3582..4187)
                     /locus_tag="ON449_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3932708.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4392..4856)
                     /locus_tag="ON449_000086"
     CDS             complement(4392..4856)
                     /locus_tag="ON449_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3932709.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4878..5198)
                     /locus_tag="ON449_000087"
     CDS             complement(4878..5198)
                     /locus_tag="ON449_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3932710.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5342..6421)
                     /gene="pepA"
                     /locus_tag="ON449_000088"
     CDS             complement(5342..6421)
                     /gene="pepA"
                     /locus_tag="ON449_000088"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3932711.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6621..6971
                     /locus_tag="ON449_000089"
     CDS             6621..6971
                     /locus_tag="ON449_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3932712.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7194..8141
                     /locus_tag="ON449_000090"
     CDS             7194..8141
                     /locus_tag="ON449_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3932713.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8198..9616)
                     /gene="pepV"
                     /locus_tag="ON449_000091"
     CDS             complement(8198..9616)
                     /gene="pepV"
                     /locus_tag="ON449_000091"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3932714.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9765..10610
                     /locus_tag="ON449_000092"
     CDS             9765..10610
                     /locus_tag="ON449_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3932715.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10614..11054
                     /locus_tag="ON449_000093"
     CDS             10614..11054
                     /locus_tag="ON449_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3932716.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11104..11298
                     /locus_tag="ON449_000094"
     CDS             11104..11298
                     /locus_tag="ON449_000094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932717.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11434..12120
                     /locus_tag="ON449_000095"
     CDS             11434..12120
                     /locus_tag="ON449_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3932718.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(12528..13217)
                     /locus_tag="ON449_000096"
     CDS             complement(12528..13217)
                     /locus_tag="ON449_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932719.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13192..15321)
                     /locus_tag="ON449_000097"
     CDS             complement(13192..15321)
                     /locus_tag="ON449_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3932720.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15369..16706)
                     /locus_tag="ON449_000098"
     CDS             complement(15369..16706)
                     /locus_tag="ON449_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3932721.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16958..17317)
                     /gene="rplT"
                     /locus_tag="ON449_000099"
     CDS             complement(16958..17317)
                     /gene="rplT"
                     /locus_tag="ON449_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3932722.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17370..17570)
                     /gene="rpmI"
                     /locus_tag="ON449_000100"
     CDS             complement(17370..17570)
                     /gene="rpmI"
                     /locus_tag="ON449_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3932723.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17642..18166)
                     /gene="infC"
                     /locus_tag="ON449_000101"
     CDS             complement(17642..18166)
                     /gene="infC"
                     /locus_tag="ON449_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3932724.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18210..18335)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18486..19118
                     /locus_tag="ON449_000102"
     CDS             18486..19118
                     /locus_tag="ON449_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3932725.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19120..20025)
                     /locus_tag="ON449_000103"
     CDS             complement(19120..20025)
                     /locus_tag="ON449_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3932726.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20110..20769)
                     /locus_tag="ON449_000104"
     CDS             complement(20110..20769)
                     /locus_tag="ON449_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3932727.1"
                     /translation="MSVIVLAGTIGAGKSSLTERMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21085..22029
                     /gene="mvk"
                     /locus_tag="ON449_000105"
     CDS             21085..22029
                     /gene="mvk"
                     /locus_tag="ON449_000105"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3932728.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22022..22999
                     /gene="mvaD"
                     /locus_tag="ON449_000106"
     CDS             22022..22999
                     /gene="mvaD"
                     /locus_tag="ON449_000106"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3932729.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23012..24097
                     /locus_tag="ON449_000107"
     CDS             23012..24097
                     /locus_tag="ON449_000107"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3932730.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24094..25158
                     /locus_tag="ON449_000108"
     CDS             24094..25158
                     /locus_tag="ON449_000108"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3932731.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25407..26210)
                     /locus_tag="ON449_000109"
     CDS             complement(25407..26210)
                     /locus_tag="ON449_000109"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3932732.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26230..27072)
                     /gene="kduI"
                     /locus_tag="ON449_000110"
     CDS             complement(26230..27072)
                     /gene="kduI"
                     /locus_tag="ON449_000110"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3932733.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27088..28095)
                     /locus_tag="ON449_000111"
     CDS             complement(27088..28095)
                     /locus_tag="ON449_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3932734.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28102..28746)
                     /locus_tag="ON449_000112"
     CDS             complement(28102..28746)
                     /locus_tag="ON449_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3932735.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28846..29604)
                     /locus_tag="ON449_000113"
     CDS             complement(28846..29604)
                     /locus_tag="ON449_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3932736.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(29731..30882)
                     /gene="fucO"
                     /locus_tag="ON449_000114"
     CDS             complement(29731..30882)
                     /gene="fucO"
                     /locus_tag="ON449_000114"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3932737.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30964..31275)
                     /gene="rhaM"
                     /locus_tag="ON449_000115"
     CDS             complement(30964..31275)
                     /gene="rhaM"
                     /locus_tag="ON449_000115"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR3932738.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31277..32122)
                     /gene="rhaD"
                     /locus_tag="ON449_000116"
     CDS             complement(31277..32122)
                     /gene="rhaD"
                     /locus_tag="ON449_000116"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR3932739.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32136..33392)
                     /gene="rhaA"
                     /locus_tag="ON449_000117"
     CDS             complement(32136..33392)
                     /gene="rhaA"
                     /locus_tag="ON449_000117"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR3932740.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33389..34849)
                     /gene="rhaB"
                     /locus_tag="ON449_000118"
     CDS             complement(33389..34849)
                     /gene="rhaB"
                     /locus_tag="ON449_000118"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR3932741.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35002..35871
                     /locus_tag="ON449_000119"
     CDS             35002..35871
                     /locus_tag="ON449_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR3932742.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35997..36896)
                     /locus_tag="ON449_000120"
     CDS             complement(35997..36896)
                     /locus_tag="ON449_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3932743.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37061..37885)
                     /locus_tag="ON449_000121"
     CDS             complement(37061..37885)
                     /locus_tag="ON449_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3932744.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37896..39191)
                     /locus_tag="ON449_000122"
     CDS             complement(37896..39191)
                     /locus_tag="ON449_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR3932745.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39207..39686)
                     /locus_tag="ON449_000123"
     CDS             complement(39207..39686)
                     /locus_tag="ON449_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3932746.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39692..40720)
                     /locus_tag="ON449_000124"
     CDS             complement(39692..40720)
                     /locus_tag="ON449_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3932747.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(40737..41843)
                     /locus_tag="ON449_000125"
     CDS             complement(40737..41843)
                     /locus_tag="ON449_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3932748.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42072..44669)
                     /gene="adhE"
                     /locus_tag="ON449_000126"
                     /gene_synonym="adhC"
     CDS             complement(42072..44669)
                     /gene="adhE"
                     /locus_tag="ON449_000126"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3932749.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44974..45354)
                     /gene="yajC"
                     /locus_tag="ON449_000127"
     CDS             complement(44974..45354)
                     /gene="yajC"
                     /locus_tag="ON449_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3932750.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45451..46596)
                     /gene="tgt"
                     /locus_tag="ON449_000128"
     CDS             complement(45451..46596)
                     /gene="tgt"
                     /locus_tag="ON449_000128"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3932751.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46814..47512
                     /locus_tag="ON449_000129"
     CDS             46814..47512
                     /locus_tag="ON449_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3932752.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47680..50319)
                     /locus_tag="ON449_000130"
     CDS             complement(47680..50319)
                     /locus_tag="ON449_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3932753.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50938..51969)
                     /gene="queA"
                     /locus_tag="ON449_000131"
     CDS             complement(50938..51969)
                     /gene="queA"
                     /locus_tag="ON449_000131"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3932754.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52162..53337
                     /locus_tag="ON449_000132"
     CDS             52162..53337
                     /locus_tag="ON449_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3932755.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53341..53982
                     /locus_tag="ON449_000133"
     CDS             53341..53982
                     /locus_tag="ON449_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932756.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53979..54896
                     /locus_tag="ON449_000134"
     CDS             53979..54896
                     /locus_tag="ON449_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3932757.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            54893..55564
                     /locus_tag="ON449_000135"
     CDS             54893..55564
                     /locus_tag="ON449_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932758.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56105..56593)
                     /locus_tag="ON449_000136"
     CDS             complement(56105..56593)
                     /locus_tag="ON449_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3932759.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(56794..57060)
                     /locus_tag="ON449_000137"
     CDS             complement(56794..57060)
                     /locus_tag="ON449_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3932760.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57060..57323)
                     /locus_tag="ON449_000138"
     CDS             complement(57060..57323)
                     /locus_tag="ON449_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3932761.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            57799..57984
                     /locus_tag="ON449_000139"
     CDS             57799..57984
                     /locus_tag="ON449_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3932762.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(58126..59949)
                     /locus_tag="ON449_000140"
     CDS             complement(58126..59949)
                     /locus_tag="ON449_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3932763.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60547..63252)
                     /locus_tag="ON449_000141"
     CDS             complement(60547..63252)
                     /locus_tag="ON449_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3932764.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63558..64505)
                     /locus_tag="ON449_000142"
     CDS             complement(63558..64505)
                     /locus_tag="ON449_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3932765.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64695..64964
                     /gene="rpsN"
                     /locus_tag="ON449_000143"
     CDS             64695..64964
                     /gene="rpsN"
                     /locus_tag="ON449_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3932766.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65140..65502)
                     /locus_tag="ON449_000144"
     CDS             complement(65140..65502)
                     /locus_tag="ON449_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3932767.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65513..66634)
                     /locus_tag="ON449_000145"
     CDS             complement(65513..66634)
                     /locus_tag="ON449_000145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3932768.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66650..67000)
                     /locus_tag="ON449_000146"
     CDS             complement(66650..67000)
                     /locus_tag="ON449_000146"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3932769.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67130..68641)
                     /locus_tag="ON449_000147"
     CDS             complement(67130..68641)
                     /locus_tag="ON449_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3932770.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68963..70315)
                     /locus_tag="ON449_000148"
     CDS             complement(68963..70315)
                     /locus_tag="ON449_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3932771.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70468..71544)
                     /locus_tag="ON449_000149"
     CDS             complement(70468..71544)
                     /locus_tag="ON449_000149"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3932772.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            71740..73485
                     /locus_tag="ON449_000150"
     CDS             71740..73485
                     /locus_tag="ON449_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3932773.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(73765..74202)
                     /locus_tag="ON449_000151"
     CDS             complement(73765..74202)
                     /locus_tag="ON449_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3932774.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74186..75559)
                     /locus_tag="ON449_000152"
     CDS             complement(74186..75559)
                     /locus_tag="ON449_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932775.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(75881..76369)
                     /locus_tag="ON449_000153"
     CDS             complement(75881..76369)
                     /locus_tag="ON449_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3932776.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76391..76993)
                     /locus_tag="ON449_000154"
     CDS             complement(76391..76993)
                     /locus_tag="ON449_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3932777.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22081 a        16482 c        14045 g        24429 t
ORIGIN      
        1 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
       61 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
      121 tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
      181 cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct
      241 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
      301 ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct
      361 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
      421 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
      481 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
      541 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
      601 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
      661 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
      721 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
      781 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
      841 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
      901 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
      961 ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt
     1021 tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
     1081 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
     1141 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
     1201 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
     1261 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
     1321 ttttaggatc aatgataaaa accgcatcac ttatgataag gcgcccccgc attgatccgg
     1381 aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt
     1441 tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag
     1501 ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta
     1561 gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt
     1621 tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct
     1681 tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag
     1741 gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt
     1801 cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt
     1861 ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt
     1921 ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct
     1981 tatttcatca cattcattag gcaaacatac gttttataag aatttatcca ctttatacac
     2041 aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt
     2101 atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt
     2161 ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca
     2221 gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag
     2281 atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg
     2341 atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat
     2401 agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc
     2461 aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt
     2521 tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag
     2581 caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc
     2641 gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat
     2701 ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt
     2761 ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca
     2821 gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt
     2881 tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac
     2941 cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca
     3001 gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt
     3061 atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta
     3121 gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa
     3181 aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt
     3241 tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc
     3301 gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc
     3361 attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat
     3421 aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca
     3481 actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat
     3541 taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct
     3601 ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa
     3661 tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc
     3721 cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca
     3781 gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc
     3841 tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca
     3901 acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat
     3961 cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt
     4021 agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc
     4081 cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata
     4141 accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca
     4201 ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc
     4261 atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg
     4321 acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa
     4381 gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa
     4441 ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt
     4501 agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc
     4561 atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat
     4621 ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact
     4681 agattttgat caataggtgc atagcccata aagttataaa attgctgatc gattacgttt
     4741 gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca
     4801 agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg
     4861 acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt
     4921 tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca
     4981 aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct
     5041 acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata
     5101 aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg
     5161 tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt
     5221 tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt
     5281 tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc
     5341 attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa
     5401 tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga
     5461 tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag
     5521 cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag
     5581 cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac
     5641 gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg
     5701 ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag
     5761 aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg
     5821 tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc
     5881 atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga
     5941 ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat
     6001 cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg
     6061 ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac
     6121 gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt
     6181 tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac
     6241 ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact
     6301 gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa
     6361 aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca
     6421 tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata
     6481 aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg
     6541 ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga
     6601 gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt
     6661 tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc
     6721 gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag
     6781 aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg
     6841 ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt
     6901 atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg
     6961 caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga
     7021 ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat
     7081 tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat
     7141 tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac
     7201 aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag
     7261 tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt
     7321 cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc
     7381 gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt
     7441 taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat
     7501 tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg
     7561 atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta
     7621 gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct
     7681 ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc
     7741 atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg
     7801 aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta
     7861 ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct
     7921 ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta
     7981 ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca cttctttcat
     8041 tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga
     8101 atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca
     8161 aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg
     8221 catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt
     8281 gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca
     8341 aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt
     8401 catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca
     8461 ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac
     8521 tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg
     8581 cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca
     8641 ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag
     8701 cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca
     8761 atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac
     8821 caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg
     8881 cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt
     8941 gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt
     9001 tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt
     9061 tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt
     9121 catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga
     9181 agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag
     9241 ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga
     9301 ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc
     9361 caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga
     9421 cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc
     9481 gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga
     9541 cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt
     9601 gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc
     9661 acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc
     9721 tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac
     9781 aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac
     9841 gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag
     9901 ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg
     9961 ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc
    10021 tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta
    10081 agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga
    10141 ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac
    10201 aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag
    10261 aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga
    10321 aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg
    10381 ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag
    10441 cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg
    10501 gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc
    10561 cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga
    10621 aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc
    10681 atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag
    10741 ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt
    10801 gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa
    10861 agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat
    10921 taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga
    10981 agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg
    11041 atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt
    11101 ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac
    11161 atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa
    11221 gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa
    11281 gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc
    11341 agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat
    11401 aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa
    11461 gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc
    11521 tccgataaag tgttagaaga acaactagct atcaggcgta caccgattcg agaagcactg
    11581 atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca
    11641 aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg
    11701 atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata
    11761 ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat
    11821 actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta
    11881 gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat ttcagaatta
    11941 gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat tgatcataac
    12001 tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga ggagcaagaa
    12061 tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc catcaaataa
    12121 gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca aaatagttcc
    12181 tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca caccgtttat
    12241 tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaatagc
    12301 ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt
    12361 ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac
    12421 agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag gagaacaccg
    12481 tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta tttgtaatct
    12541 aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat cccttggcta
    12601 cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga aaaatcgctt
    12661 tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg aatgtttgat
    12721 tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt ataaattccc
    12781 ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt
    12841 tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc cgtcatcaat
    12901 cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat ttgctctgat
    12961 tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc ggcaaaaggg
    13021 tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg ttgtataaga
    13081 attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa ctggtagaat
    13141 ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa cttatgtttg
    13201 tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag atacttccag
    13261 atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga ctcttgctcc
    13321 taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg ccatatcttc
    13381 ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac atgaagcatg
    13441 tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc cagcattttt
    13501 tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata agcaaacttc
    13561 atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg ttgtgatctc
    13621 cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg ttacttttga
    13681 agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc ctgattcaat
    13741 cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg gcgaagggat
    13801 gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag aaatcacttc
    13861 ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag taatcaatga
    13921 ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt gatacttgtc
    13981 tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga tctgttctgc
    14041 gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt ttcgtgcaga
    14101 tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat tggctgcaca
    14161 tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt aatcagcagg
    14221 gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt ctgtctccgt
    14281 aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat cgatgattcc
    14341 tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa catctaatga
    14401 agagatatat gttgggtggc cacctacaat tgaataatcg attgctggac gtccattttt
    14461 aatgaccccg atattggttg ttgttccgcc tacttcaaaa taaacaccat tagacgctct
    14521 caagtacatc aaagagccca tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg
    14581 tcgttttttc atttcattga tttccattac tccgccgtct ccgcgcataa tcatcaaaga
    14641 aacattgacc cctgcttctc gtacggaatc ttctgtagag gttgccgtat ctagcatttt
    14701 tggtaaaata gatgcattga ttgctgctgt ccgagttctt cttgtcaatc catataattt
    14761 tgtgatgtca gaagccatcg ttgtagggat cgattttacc gatgctgctt cataaacaac
    14821 tcgttctggt tccccattat ctactccaaa tgccatagaa gaaaccaaga cctccgctct
    14881 ttctctttct aaagaagaaa tcgtttctga aacacggtct acatttaagt gtttgacagg
    14941 taagaatgca ttgacaatct ctattttttt cttattccct aaatcgatat cgttcaatct
    15001 agtttgtcgt tttgctaaaa aaccttctaa tccacctttt gccatcccga ttacgccgac
    15061 ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa
    15121 cacaacatct tctggactga tattattttc cagcaaacaa ttttgaaaag actgaacgac
    15181 acctgcggct actccacgga catcatcgtg ggttgttttc actgaagatt ttccgatgat
    15241 ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc
    15301 cattcgcaca aaacgtttca ttttcttttc ctccattcat ctaatcgttt ttatcgacgt
    15361 tttgtttact aggcgttccc gtacaataag aacgtgatca ggcataagat cgctccgatg
    15421 atccaaccag ttgggatgga tttcttcata tattctcgag aagttacttt tgtataacct
    15481 aatccccaag caacccaaga ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt
    15541 gcaaataacg ggaacaaaaa ggctggcgac atcgttggat taacagctaa tacgacagct
    15601 aaaatagctg agcctgaacc gactagattc attggaccgc ggaaaaatcc tagtggtgtc
    15661 aatatagcaa atacgattgc caagactaat ggtgattgcg gtatccaatc tccaaaaata
    15721 gctgaaaaat aaggagaagc atacgcagct gaattattga acatcgctaa ggtcatcaag
    15781 aaaccaacca ttggcgcaac atctactgcg ccatctgtaa ataatttcgc aaactgacga
    15841 cagatttctt tataacctcc gcgcaatttt ccagtaaaca gcaatgccca gatacctgct
    15901 agaataaaac ctaagatgat tggaagatct aaaagaacga cccctaaaac tggtaataat
    15961 acggctaacc atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt
    16021 gcatagcgtt tctttttgtt cattgatata ttagcgaccg ttagtaccac aactaagctt
    16081 acaatcatcc caatcatacc aatttggaaa tagtcattat aagtatagct ttcagccgct
    16141 ggattgaaac cagcataaat cgtttgatat tgtttgaaat tgacgatatt agcaaagatt
    16201 ccagccataa tcgagcccat aaatgagaac agtgcaaccg gtactgaaat acctaatgac
    16261 atcaagattg gcaacacaat aactgcaata gaaatcactg gtccgattcc tgtcatcgac
    16321 atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg cttgtcccct
    16381 ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac tagaacacgc
    16441 ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc agctggcccc
    16501 gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc cgggtttggt
    16561 gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat ggcaaagccg
    16621 accatcaagt tgtagccttt tacacagtac cagaccatga aaaagaatga gataactaac
    16681 aaaataccaa taataatatc actcatgtta ttcctccgtc cattttgttt ttctgataag
    16741 caaactaata tatcagtcaa actcattcta acttatgtag ttagcgcttt cattaaaggg
    16801 tgaccataaa tgttcagttt tcaattaaaa aaccttaatt atgctatttt agacttatac
    16861 gttattgtcc tgtttcattt gatggaggat tgtaaataaa aaaagaggaa agaacataac
    16921 ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg
    16981 cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt
    17041 tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc
    17101 cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct
    17161 tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt
    17221 taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac
    17281 gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat
    17341 aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata
    17401 cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt
    17461 gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca
    17521 gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt
    17581 cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa
    17641 attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg
    17701 ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt
    17761 gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat
    17821 cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga
    17881 tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc
    17941 ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg
    18001 gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg
    18061 cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc
    18121 gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat
    18181 tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga
    18241 acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca
    18301 actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccatcatagt atagcaaaaa
    18361 ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat
    18421 tagcagactt tctagcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa
    18481 cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt
    18541 tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct
    18601 atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa
    18661 aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca
    18721 atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag
    18781 gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga
    18841 tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag
    18901 acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg
    18961 gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag
    19021 caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg
    19081 tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag
    19141 tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa
    19201 aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg
    19261 aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt
    19321 cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat
    19381 ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga
    19441 caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt
    19501 tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt
    19561 cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat
    19621 taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact
    19681 catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg
    19741 cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata
    19801 atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa
    19861 agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc
    19921 actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt
    19981 gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga
    20041 catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc
    20101 aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat
    20161 tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat
    20221 ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc
    20281 tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt
    20341 ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg
    20401 ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac
    20461 acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc
    20521 ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt ttatgcttgc
    20581 aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata
    20641 aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg
    20701 ttcagccatc ctttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat
    20761 cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt
    20821 taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa
    20881 aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt
    20941 acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca
    21001 aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac
    21061 ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag
    21121 atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc
    21181 tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt
    21241 tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa
    21301 ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa
    21361 agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact
    21421 cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc
    21481 cagctttcgg aaaaaatcgc ccacggtaat cctagtggaa tcgatgcagc cgctactagc
    21541 agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc
    21601 gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa
    21661 gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta
    21721 ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca
    21781 cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt
    21841 aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt
    21901 ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt
    21961 caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc
    22021 tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa
    22081 gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt
    22141 ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga
    22201 tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg
    22261 taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc
    22321 agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc
    22381 tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc
    22441 agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag
    22501 ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt
    22561 tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga
    22621 aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa
    22681 acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact
    22741 aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag
    22801 cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac
    22861 gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca
    22921 agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg
    22981 aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact
    23041 ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt
    23101 cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc
    23161 acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat
    23221 tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca
    23281 agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc
    23341 aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc
    23401 cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact
    23461 agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg
    23521 tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca
    23581 ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc
    23641 tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt
    23701 ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa
    23761 aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt
    23821 gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg
    23881 ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg
    23941 agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa
    24001 atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt
    24061 acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca
    24121 ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat
    24181 aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt
    24241 acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa
    24301 atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca
    24361 gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa
    24421 tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa
    24481 gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag
    24541 ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact
    24601 caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa
    24661 acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga
    24721 acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct
    24781 gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg
    24841 gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc
    24901 ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta
    24961 gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg
    25021 gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg
    25081 aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag
    25141 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg
    25201 aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
    25261 cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat
    25321 aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct
    25381 ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct
    25441 ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca
    25501 gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata
    25561 aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc
    25621 ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg accgtattca
    25681 gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc
    25741 gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc gccgcctttt
    25801 tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg
    25861 attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct
    25921 gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct
    25981 tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct
    26041 ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt
    26101 gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg
    26161 actaatgcta cttttccatc taaacgaaac atatccatac taaattccat cattttctct
    26221 cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt
    26281 ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga
    26341 ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg
    26401 tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc
    26461 ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa
    26521 aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta
    26581 gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat
    26641 actgattttt acatttggat atttatgatg agctggaaca gaactaatat agaatttagc
    26701 tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta
    26761 tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc
    26821 gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc
    26881 taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt
    26941 gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt
    27001 tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc
    27061 catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa
    27121 aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa
    27181 ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc
    27241 atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat
    27301 tctatggact tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt
    27361 ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt
    27421 ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc
    27481 gatacctaac aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa
    27541 aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat
    27601 atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt
    27661 ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg
    27721 gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg
    27781 atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag
    27841 tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt
    27901 gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta
    27961 attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc
    28021 acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt
    28081 cactactttt cccataagca gctacctctt gatttctttc atttttgctg catactgttg
    28141 cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc
    28201 tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc
    28261 gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg
    28321 tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt
    28381 gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc
    28441 tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa
    28501 gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt
    28561 gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc
    28621 aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct
    28681 ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt
    28741 tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata
    28801 cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct
    28861 ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc
    28921 tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac
    28981 gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg
    29041 ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc
    29101 tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc
    29161 tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga
    29221 agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt
    29281 gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg
    29341 taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt
    29401 tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt
    29461 atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa
    29521 agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt
    29581 tccataaggc tttttatttt ccatataata ttctcctatt tctttataag aaaaattata
    29641 gcggaaatac catcaattta aaagagatat ccagatataa actaccattt ctctttacag
    29701 gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat
    29761 ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc
    29821 tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc
    29881 aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga
    29941 catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt
    30001 atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg
    30061 aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt
    30121 tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc
    30181 gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat
    30241 atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc
    30301 catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa
    30361 cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc
    30421 tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa
    30481 tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa
    30541 ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc
    30601 gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt
    30661 ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc
    30721 tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac
    30781 agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt
    30841 tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt
    30901 tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg
    30961 ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt
    31021 ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt
    31081 ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc
    31141 aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca
    31201 aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa
    31261 agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt
    31321 ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca
    31381 agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga
    31441 tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata
    31501 aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg
    31561 gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca
    31621 tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta
    31681 atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg
    31741 cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc
    31801 cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg
    31861 caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc
    31921 ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca
    31981 agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat
    32041 cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt
    32101 gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct
    32161 ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa
    32221 cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt
    32281 cttgaagtaa agtcaaaagc cagctccatt tcttccaatc gctcaactgg ttccagcata
    32341 gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg
    32401 atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact
    32461 aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa
    32521 cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat
    32581 ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaaac aagtttgtca
    32641 cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct
    32701 aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta
    32761 aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta
    32821 tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca
    32881 aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct
    32941 ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga
    33001 ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc
    33061 ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc
    33121 aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca
    33181 ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca
    33241 tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg
    33301 tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt
    33361 gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt
    33421 accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact
    33481 gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca
    33541 tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat
    33601 aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct
    33661 tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag
    33721 caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg
    33781 tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt
    33841 cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg
    33901 cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga
    33961 tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa
    34021 aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac
    34081 tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat
    34141 catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt
    34201 tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga
    34261 caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca
    34321 tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat
    34381 caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt
    34441 ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag
    34501 aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg
    34561 gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga
    34621 tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct
    34681 ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt
    34741 tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta
    34801 gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc
    34861 ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct
    34921 atcataatac agaatcttat attttgcttc acactatttt ggtatataat tgcattatct
    34981 tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa
    35041 gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc
    35101 caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct
    35161 tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag
    35221 caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa
    35281 acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa
    35341 tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca
    35401 tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat
    35461 gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg
    35521 caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag
    35581 caatacttag acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc
    35641 ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc
    35701 gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt
    35761 acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg
    35821 ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa
    35881 tatgtacgtc attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag
    35941 tttctgcttc tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat
    36001 tgttcatctt caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat
    36061 tctttaggcg ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa
    36121 aaaccacttt tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca
    36181 tagatcagct ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat
    36241 tctcgtgtga tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc
    36301 ggatgagata aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact
    36361 acagcattct gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag
    36421 tattctacca gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga
    36481 tggtcgataa atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc
    36541 ctcttctctc ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt
    36601 tctagaaaag aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg
    36661 acgatacgat gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat
    36721 tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa
    36781 tcatgatgat gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca
    36841 tactgatcag aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc
    36901 cgcacctcct tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa
    36961 agagctgctt ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc
    37021 cctccattct aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc
    37081 cataacgaat cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct
    37141 tcatgatgat ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca
    37201 cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa
    37261 aacgttcaaa agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca
    37321 aattggtcgg atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct
    37381 ctacccttgc taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt
    37441 ggtatttttc tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt
    37501 catctgtgaa attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag
    37561 caaccatcga cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca
    37621 actcttgtcg tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta
    37681 cggtcacttc ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct
    37741 catttgcgcc agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa
    37801 tcccttgttc ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat
    37861 gtcctctaat tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac
    37921 atgacgattt gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa
    37981 ggaagaagcg gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat
    38041 agtcctgtac ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac
    38101 ccgtaatgta caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa
    38161 atcaagatag caggcgccac atccataaac atacccacca gtaataacac aagattgact
    38221 aataacagaa taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga
    38281 ggaataccgg taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg
    38341 atcgtccctg acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc
    38401 gttttatagt aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct
    38461 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    38521 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    38581 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg
    38641 cctacgagat aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa
    38701 tacatgataa aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg
    38761 accgctgtcg caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg
    38821 ccaatcgcgg tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct
    38881 aacacattcg tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc
    38941 actagtttct tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga
    39001 atagcgatca gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca
    39061 gaaacatcaa acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata
    39121 gcaatcggca tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct
    39181 tctattgcca ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt
    39241 aatgtatcga ttagattgat caggctatag ataatgatga gtacccccga tacaggtact
    39301 gacagataaa catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat
    39361 gtgacagcat tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt
    39421 tgaacaacaa cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt
    39481 acggctaaat gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca
    39541 agaccgaatc gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa
    39601 aatacttgcc ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct
    39661 aataccttgt ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat
    39721 aaaggtcttt gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc
    39781 gaaacggtgt attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt
    39841 tttcttcttc aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg
    39901 cttcttcaat ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga
    39961 tgatgatatc tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat
    40021 gtccagctgt atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa
    40081 gagacgtgta cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca
    40141 ttttgatagc ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg
    40201 tatggatcgg accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc
    40261 cggtaaatcc taaatttttg gtcgattggt agattggctc cattatttct tcattatcca
    40321 tcaccttaaa gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat
    40381 ctttagagaa actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact
    40441 gtgtcaattc gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct
    40501 gtacggaacc atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta
    40561 ctggctggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat
    40621 ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc
    40681 taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa
    40741 caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt
    40801 gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa
    40861 acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa
    40921 gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa
    40981 gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc
    41041 cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg
    41101 atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga
    41161 atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt
    41221 ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata
    41281 tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg
    41341 atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt
    41401 tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg
    41461 acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac
    41521 aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct
    41581 tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc
    41641 tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag
    41701 cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg
    41761 atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca
    41821 aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc
    41881 cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa
    41941 aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga
    42001 acttggagaa acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg
    42061 ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat
    42121 ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct
    42181 acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg
    42241 acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt
    42301 tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag
    42361 tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc
    42421 ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt
    42481 gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa
    42541 gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag
    42601 tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact
    42661 gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat
    42721 gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa
    42781 tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact
    42841 tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg
    42901 atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg
    42961 tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca
    43021 agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta
    43081 tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt
    43141 cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa
    43201 acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca
    43261 aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt
    43321 ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa
    43381 ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct
    43441 gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc
    43501 aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata
    43561 ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga
    43621 gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc
    43681 attttcgttc cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt
    43741 ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt
    43801 tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct
    43861 gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca
    43921 aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata
    43981 taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac
    44041 ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc
    44101 atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg
    44161 gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt
    44221 ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc
    44281 gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca
    44341 atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg
    44401 atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca
    44461 ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc
    44521 attgccattt catgcacgat atggtctact ttttcttgat caaagctttc catttctttc
    44581 aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc
    44641 gttttttctt tttctattac tttagccatc attatcctcc taatgggtca tggttatttg
    44701 ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg
    44761 atttttttca cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa
    44821 aaatatataa agttatttat tattatttat atgacacacc cgattattca atattgagca
    44881 atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc
    44941 tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc
    45001 ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt
    45061 ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc
    45121 tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt
    45181 gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt
    45241 catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa
    45301 aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt
    45361 cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag
    45421 ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa
    45481 cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg
    45541 acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat
    45601 cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca
    45661 tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt
    45721 cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa
    45781 gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc
    45841 gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac
    45901 acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa
    45961 atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg
    46021 gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc
    46081 ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg
    46141 acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt
    46201 ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc
    46261 ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat
    46321 cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc
    46381 agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa
    46441 ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt
    46501 ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt
    46561 cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa
    46621 atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc
    46681 ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa
    46741 gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa
    46801 ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa
    46861 aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac
    46921 tattcttctt attatgattc ctattatctt gtttgccacc accatatatg atggatcagc
    46981 gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat
    47041 ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt
    47101 acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt
    47161 gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg
    47221 tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat
    47281 ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc
    47341 aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat
    47401 cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata
    47461 catttctgca acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt
    47521 gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa
    47581 cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta
    47641 tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc
    47701 cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc
    47761 taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg
    47821 cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt
    47881 attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag
    47941 aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc
    48001 tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat
    48061 ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc
    48121 atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc
    48181 aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg
    48241 tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct
    48301 tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc
    48361 gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac
    48421 atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc
    48481 attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat
    48541 ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc
    48601 taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt
    48661 caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat
    48721 gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg
    48781 tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga
    48841 aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg
    48901 tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg
    48961 agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac
    49021 tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc
    49081 ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt
    49141 tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag
    49201 tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac
    49261 ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt
    49321 tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat
    49381 gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac
    49441 agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag
    49501 gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat
    49561 catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt
    49621 attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac
    49681 gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc
    49741 acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc
    49801 tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc
    49861 agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc
    49921 aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa
    49981 ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac
    50041 aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt
    50101 ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg
    50161 aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt
    50221 ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac
    50281 cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct
    50341 tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa
    50401 gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc
    50461 taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga
    50521 atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac
    50581 atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt
    50641 atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg
    50701 aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa
    50761 catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt
    50821 tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt
    50881 gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta
    50941 tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg
    51001 gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga
    51061 ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt
    51121 aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat
    51181 cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg
    51241 tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg
    51301 gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt
    51361 cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg
    51421 caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg
    51481 atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc
    51541 aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa
    51601 ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt
    51661 ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag
    51721 aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac
    51781 tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa
    51841 atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg
    51901 gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt
    51961 tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt
    52021 tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact
    52081 accctatttg tattttttca ttgttttagg catactagta atagatagtt cttcattgaa
    52141 aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt
    52201 ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt
    52261 atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat
    52321 ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa
    52381 ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc
    52441 cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa
    52501 acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca
    52561 catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat
    52621 caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc
    52681 ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc
    52741 catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt
    52801 gtgatccaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa
    52861 gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa
    52921 gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa
    52981 ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa
    53041 ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac
    53101 attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa
    53161 cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta
    53221 ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat
    53281 cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct
    53341 atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt
    53401 tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta
    53461 ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca
    53521 atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct
    53581 gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg
    53641 atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc
    53701 caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt
    53761 ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt
    53821 ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt
    53881 acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac
    53941 cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga
    54001 ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca
    54061 atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag
    54121 caagtctagt tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca
    54181 atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag
    54241 cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc
    54301 cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt
    54361 tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa
    54421 aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg
    54481 tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg
    54541 gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag
    54601 caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc
    54661 tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag
    54721 acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata
    54781 ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac
    54841 catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca
    54901 attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta
    54961 tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc
    55021 agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt
    55081 gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat
    55141 cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt
    55201 accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt
    55261 cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt taccactctc
    55321 tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc
    55381 gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc
    55441 aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat
    55501 cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg
    55561 ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt
    55621 tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga
    55681 gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt
    55741 catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat
    55801 tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt
    55861 ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca
    55921 tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg
    55981 gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac
    56041 atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg
    56101 attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa
    56161 gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa
    56221 agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca
    56281 cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc
    56341 gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc
    56401 atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt
    56461 tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt
    56521 ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga
    56581 ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta
    56641 aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa
    56701 taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt
    56761 gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata
    56821 aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag
    56881 cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat
    56941 atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg
    57001 cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat
    57061 caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg
    57121 tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc
    57181 cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt
    57241 tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta
    57301 ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac
    57361 ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt
    57421 ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac
    57481 tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc
    57541 aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg
    57601 ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa
    57661 cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc
    57721 tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt
    57781 gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag
    57841 gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct
    57901 gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc
    57961 aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac gctgttccca
    58021 tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc
    58081 ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg
    58141 gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa
    58201 acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa
    58261 cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat
    58321 aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa
    58381 atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac
    58441 atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac
    58501 ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc
    58561 atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa
    58621 aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac
    58681 aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt
    58741 gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc
    58801 aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa
    58861 aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag
    58921 tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc
    58981 aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc
    59041 aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa
    59101 aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac
    59161 gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct
    59221 tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga
    59281 accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg
    59341 aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat
    59401 cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc
    59461 cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac
    59521 ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga
    59581 tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt
    59641 ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat
    59701 gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg
    59761 tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt
    59821 accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt
    59881 taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa
    59941 gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat
    60001 ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga
    60061 aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa
    60121 aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg
    60181 acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg
    60241 ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga
    60301 taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag
    60361 aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct
    60421 tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg
    60481 aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct
    60541 ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc
    60601 ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat
    60661 gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa
    60721 gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac
    60781 ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat
    60841 ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa
    60901 ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa
    60961 gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag
    61021 catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata
    61081 gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt
    61141 ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag
    61201 ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat
    61261 gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat
    61321 accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt
    61381 ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag
    61441 tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg
    61501 taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt
    61561 tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg
    61621 atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga
    61681 atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat
    61741 gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat
    61801 gatgccattg atattattaa caacggcatc aatttgatct agcaattcat caatagcctt
    61861 cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag
    61921 caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact
    61981 tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat
    62041 tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt
    62101 accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg
    62161 tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag
    62221 ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt
    62281 cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc
    62341 gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg
    62401 cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg
    62461 atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac
    62521 tttagggatg taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag
    62581 ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc
    62641 tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta
    62701 acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt
    62761 gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat
    62821 tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag
    62881 cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac
    62941 tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg
    63001 aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt
    63061 gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc
    63121 tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt
    63181 tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt
    63241 gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt
    63301 tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa
    63361 ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta
    63421 tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg
    63481 caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt
    63541 ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt
    63601 ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa
    63661 ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca
    63721 tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa
    63781 tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg
    63841 ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca
    63901 aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt
    63961 accttttcct catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga
    64021 agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc
    64081 ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt
    64141 ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac
    64201 acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga
    64261 acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca
    64321 taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa
    64381 ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga
    64441 tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt
    64501 cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa
    64561 aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa
    64621 cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt
    64681 ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa
    64741 cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct
    64801 tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg
    64861 gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag
    64921 cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta
    64981 atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt
    65041 cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc
    65101 ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag
    65161 tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc
    65221 caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag
    65281 gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag
    65341 cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt
    65401 acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac
    65461 tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt
    65521 gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta
    65581 attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca
    65641 cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac
    65701 gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg
    65761 gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat
    65821 atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag
    65881 aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat
    65941 taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata
    66001 aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca
    66061 cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag
    66121 aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg
    66181 cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta
    66241 gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca
    66301 accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt
    66361 cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga
    66421 ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag
    66481 ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc
    66541 cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca
    66601 ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca
    66661 aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt
    66721 catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt
    66781 gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt
    66841 aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt
    66901 catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc
    66961 taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata
    67021 aatttagaac actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa
    67081 taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat
    67141 ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc
    67201 tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac
    67261 gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca
    67321 ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg
    67381 tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg
    67441 ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg
    67501 cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc
    67561 gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag
    67621 tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt
    67681 ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac
    67741 gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata
    67801 cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg
    67861 cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta
    67921 attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg
    67981 cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat
    68041 gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg
    68101 agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca
    68161 agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa
    68221 gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat
    68281 gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt
    68341 gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta
    68401 attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt
    68461 ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa
    68521 cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa
    68581 aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca
    68641 aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt
    68701 tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct
    68761 tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa
    68821 cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg
    68881 caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg
    68941 ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata
    69001 agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga
    69061 tagcatcaat caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt
    69121 tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga
    69181 atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta
    69241 attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag
    69301 tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg
    69361 caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc
    69421 cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag
    69481 cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa
    69541 aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac
    69601 cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg
    69661 cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag
    69721 cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg
    69781 cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca
    69841 tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa
    69901 tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg
    69961 cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc
    70021 ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag
    70081 gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg
    70141 ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa
    70201 cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca
    70261 gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga
    70321 tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc
    70381 tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa
    70441 tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg
    70501 atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc
    70561 catattttcc cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa
    70621 ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct
    70681 tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc
    70741 tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg cttcataatc
    70801 atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc
    70861 taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc
    70921 atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat
    70981 gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc
    71041 acattgatca aatacttttt taggattttc tttccattga ttcttaagta ctggtacata
    71101 aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc
    71161 acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc
    71221 ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc
    71281 ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact
    71341 tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc
    71401 tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata
    71461 aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc
    71521 gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat
    71581 ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga
    71641 gccagcttct aaaagaacga acgtcattta ttttttgtca gctagattat ttgtcccgtt
    71701 ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc
    71761 agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta
    71821 cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc
    71881 aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca
    71941 ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc
    72001 gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt
    72061 gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc
    72121 gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa
    72181 cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta
    72241 ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat
    72301 ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat
    72361 gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat
    72421 catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag
    72481 ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat
    72541 ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa
    72601 gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt
    72661 gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc
    72721 gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt
    72781 ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag
    72841 caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag
    72901 tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg
    72961 atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt
    73021 gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct
    73081 tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa
    73141 agcggattag acttattgga agggatgaag gctgcgccta agatccctca acagctccac
    73201 catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa
    73261 gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta
    73321 gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag
    73381 gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg
    73441 gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca
    73501 gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg
    73561 tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg
    73621 aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta
    73681 tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct
    73741 actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga
    73801 catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa
    73861 ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt
    73921 tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc
    73981 ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc
    74041 agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg
    74101 gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc
    74161 ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc
    74221 ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca
    74281 agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata
    74341 gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact
    74401 ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg
    74461 gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc
    74521 agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag
    74581 aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc
    74641 atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg
    74701 aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat
    74761 tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct
    74821 tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag
    74881 caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc
    74941 caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt
    75001 tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa
    75061 gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat
    75121 acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac
    75181 cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca
    75241 gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa
    75301 gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac
    75361 caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa
    75421 atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa
    75481 taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc
    75541 gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt
    75601 cgattttagc attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa
    75661 atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga
    75721 caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata
    75781 gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct
    75841 cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca
    75901 ttttataatc gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct
    75961 caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa
    76021 agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag
    76081 tcaatccttg accacgtact ttatttagca aatataattt acttaaataa atttttccat
    76141 ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg
    76201 acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt
    76261 aatccacttg tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga
    76321 ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc
    76381 cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat
    76441 acacacccac cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata
    76501 atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg
    76561 taacaactag tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct
    76621 gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca
    76681 aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat
    76741 tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca
    76801 cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa
    76861 acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca
    76921 caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc
    76981 cttttttaga catctatatt ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DAJSVP010000003        73660 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSVP010000003 DAJSVP010000000
VERSION     DAJSVP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.003"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            100..1224
                     /locus_tag="ON449_000155"
     CDS             100..1224
                     /locus_tag="ON449_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3932778.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1451..2368
                     /locus_tag="ON449_000156"
     CDS             1451..2368
                     /locus_tag="ON449_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3932779.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2361..3194
                     /locus_tag="ON449_000157"
     CDS             2361..3194
                     /locus_tag="ON449_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3932780.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3277..4395
                     /locus_tag="ON449_000158"
     CDS             3277..4395
                     /locus_tag="ON449_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3932781.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4389..6287
                     /locus_tag="ON449_000159"
     CDS             4389..6287
                     /locus_tag="ON449_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3932782.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6281..7504
                     /locus_tag="ON449_000160"
     CDS             6281..7504
                     /locus_tag="ON449_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3932783.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7590..9524)
                     /locus_tag="ON449_000161"
     CDS             complement(7590..9524)
                     /locus_tag="ON449_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3932784.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9750..10406
                     /locus_tag="ON449_000162"
     CDS             9750..10406
                     /locus_tag="ON449_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3932785.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10480..10833)
                     /locus_tag="ON449_000163"
     CDS             complement(10480..10833)
                     /locus_tag="ON449_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3932786.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11025..11954
                     /locus_tag="ON449_000164"
     CDS             11025..11954
                     /locus_tag="ON449_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3932787.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11965..12801
                     /locus_tag="ON449_000165"
     CDS             11965..12801
                     /locus_tag="ON449_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3932788.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13049..13825
                     /locus_tag="ON449_000166"
     CDS             13049..13825
                     /locus_tag="ON449_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3932789.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14001..14744
                     /locus_tag="ON449_000167"
     CDS             14001..14744
                     /locus_tag="ON449_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932790.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14737..16317
                     /locus_tag="ON449_000168"
     CDS             14737..16317
                     /locus_tag="ON449_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3932791.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16568..17047)
                     /locus_tag="ON449_000169"
     CDS             complement(16568..17047)
                     /locus_tag="ON449_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3932792.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17063..17815)
                     /locus_tag="ON449_000170"
     CDS             complement(17063..17815)
                     /locus_tag="ON449_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3932793.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17932..18002
                     /locus_tag="ON449_000171"
     tRNA            17932..18002
                     /locus_tag="ON449_000171"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17964..17966,aa:Gly,seq:ccc)
     gene            18368..19093
                     /locus_tag="ON449_000172"
     CDS             18368..19093
                     /locus_tag="ON449_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3932794.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19347..19613
                     /locus_tag="ON449_000173"
     CDS             19347..19613
                     /locus_tag="ON449_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3932795.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDSAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19610..20041
                     /gene="ruvX"
                     /locus_tag="ON449_000174"
     CDS             19610..20041
                     /gene="ruvX"
                     /locus_tag="ON449_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3932796.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20067..20369
                     /locus_tag="ON449_000175"
     CDS             20067..20369
                     /locus_tag="ON449_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3932797.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            20559..21395
                     /locus_tag="ON449_000176"
     CDS             20559..21395
                     /locus_tag="ON449_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3932798.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            21459..21890
                     /locus_tag="ON449_000177"
     CDS             21459..21890
                     /locus_tag="ON449_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3932799.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(21952..23274)
                     /locus_tag="ON449_000178"
     CDS             complement(21952..23274)
                     /locus_tag="ON449_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3932800.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(23428..25068)
                     /locus_tag="ON449_000179"
     CDS             complement(23428..25068)
                     /locus_tag="ON449_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3932801.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            complement(25509..26828)
                     /locus_tag="ON449_000180"
     CDS             complement(25509..26828)
                     /locus_tag="ON449_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCR3932802.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            27085..27555
                     /locus_tag="ON449_000181"
     CDS             27085..27555
                     /locus_tag="ON449_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3932803.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            27777..28880
                     /locus_tag="ON449_000182"
     CDS             27777..28880
                     /locus_tag="ON449_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3932804.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            29101..30060
                     /locus_tag="ON449_000183"
     CDS             29101..30060
                     /locus_tag="ON449_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3932805.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            30230..30910
                     /locus_tag="ON449_000184"
     CDS             30230..30910
                     /locus_tag="ON449_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3932806.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            31000..32034
                     /locus_tag="ON449_000185"
     CDS             31000..32034
                     /locus_tag="ON449_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3932807.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            32048..32383
                     /locus_tag="ON449_000186"
     CDS             32048..32383
                     /locus_tag="ON449_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932808.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            32367..34847
                     /locus_tag="ON449_000187"
     CDS             32367..34847
                     /locus_tag="ON449_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932809.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            34903..36462
                     /locus_tag="ON449_000188"
     CDS             34903..36462
                     /locus_tag="ON449_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3932810.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            36459..37823
                     /locus_tag="ON449_000189"
     CDS             36459..37823
                     /locus_tag="ON449_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3932811.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            37994..39064
                     /locus_tag="ON449_000190"
     CDS             37994..39064
                     /locus_tag="ON449_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932812.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            39079..39747
                     /locus_tag="ON449_000191"
     CDS             39079..39747
                     /locus_tag="ON449_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932813.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            39767..40345
                     /locus_tag="ON449_000192"
     CDS             39767..40345
                     /locus_tag="ON449_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3932814.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            40746..41657
                     /locus_tag="ON449_000193"
     CDS             40746..41657
                     /locus_tag="ON449_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3932815.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(42035..42952)
                     /locus_tag="ON449_000194"
     CDS             complement(42035..42952)
                     /locus_tag="ON449_000194"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3932816.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            43154..43591
                     /gene="zapA"
                     /locus_tag="ON449_000195"
     CDS             43154..43591
                     /gene="zapA"
                     /locus_tag="ON449_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3932817.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            43649..44197
                     /locus_tag="ON449_000196"
     CDS             43649..44197
                     /locus_tag="ON449_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3932818.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            44312..46672
                     /locus_tag="ON449_000197"
     CDS             44312..46672
                     /locus_tag="ON449_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3932819.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            46759..47073
                     /gene="trxA"
                     /locus_tag="ON449_000198"
     CDS             46759..47073
                     /gene="trxA"
                     /locus_tag="ON449_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3932820.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            47255..49033
                     /gene="uvrC"
                     /locus_tag="ON449_000199"
     CDS             47255..49033
                     /gene="uvrC"
                     /locus_tag="ON449_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3932821.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            49178..50077
                     /locus_tag="ON449_000200"
     CDS             49178..50077
                     /locus_tag="ON449_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932822.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            50070..51332
                     /locus_tag="ON449_000201"
     CDS             50070..51332
                     /locus_tag="ON449_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932823.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            51493..53391
                     /locus_tag="ON449_000202"
     CDS             51493..53391
                     /locus_tag="ON449_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3932824.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(53721..54440)
                     /locus_tag="ON449_000203"
     CDS             complement(53721..54440)
                     /locus_tag="ON449_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3932825.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            54777..55109
                     /locus_tag="ON449_000204"
     CDS             54777..55109
                     /locus_tag="ON449_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932826.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            55096..57099
                     /locus_tag="ON449_000205"
     CDS             55096..57099
                     /locus_tag="ON449_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3932827.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            57118..57588
                     /locus_tag="ON449_000206"
     CDS             57118..57588
                     /locus_tag="ON449_000206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3932828.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            57609..58193
                     /locus_tag="ON449_000207"
     CDS             57609..58193
                     /locus_tag="ON449_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3932829.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            58208..59194
                     /locus_tag="ON449_000208"
     CDS             58208..59194
                     /locus_tag="ON449_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3932830.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            59184..59495
                     /locus_tag="ON449_000209"
     CDS             59184..59495
                     /locus_tag="ON449_000209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3932831.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            59517..61298
                     /locus_tag="ON449_000210"
     CDS             59517..61298
                     /locus_tag="ON449_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3932832.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            61291..62667
                     /locus_tag="ON449_000211"
     CDS             61291..62667
                     /locus_tag="ON449_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3932833.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            62674..63309
                     /locus_tag="ON449_000212"
     CDS             62674..63309
                     /locus_tag="ON449_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3932834.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            63339..64694
                     /locus_tag="ON449_000213"
     CDS             63339..64694
                     /locus_tag="ON449_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3932835.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(64886..66280)
                     /locus_tag="ON449_000214"
     CDS             complement(64886..66280)
                     /locus_tag="ON449_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3932836.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            66372..67343
                     /locus_tag="ON449_000215"
     CDS             66372..67343
                     /locus_tag="ON449_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3932837.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            67340..68122
                     /locus_tag="ON449_000216"
     CDS             67340..68122
                     /locus_tag="ON449_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3932838.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(68291..68662)
                     /locus_tag="ON449_000217"
     CDS             complement(68291..68662)
                     /locus_tag="ON449_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3932839.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            68796..69239
                     /locus_tag="ON449_000218"
     CDS             68796..69239
                     /locus_tag="ON449_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3932840.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(69282..70091)
                     /gene="yidA"
                     /locus_tag="ON449_000219"
     CDS             complement(69282..70091)
                     /gene="yidA"
                     /locus_tag="ON449_000219"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3932841.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(70110..71477)
                     /locus_tag="ON449_000220"
     CDS             complement(70110..71477)
                     /locus_tag="ON449_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3932842.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(71461..72291)
                     /locus_tag="ON449_000221"
     CDS             complement(71461..72291)
                     /locus_tag="ON449_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3932843.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            72454..72891
                     /locus_tag="ON449_000222"
     CDS             72454..72891
                     /locus_tag="ON449_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3932844.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            72967..73587
                     /locus_tag="ON449_000223"
     CDS             72967..73587
                     /locus_tag="ON449_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3932845.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        24002 a        12616 c        15285 g        21757 t
ORIGIN      
        1 aaaatatttt tttatataaa tatttgtgtt actaattccg ttaatataca taagtgaaag
       61 cgttttatat aagaacttga aaagaaagaa gggatagaca tgaaaaaaag atacaaattg
      121 gtgatagctt tgctaaacat taccctatta ttcagcttaa gtgcatgtgg aaaaactaac
      181 gaagaagtaa cagcaaacat cgcagatcca atgataaaaa aagccactac attagaagta
      241 tatgtcgcaa acggcaaact gacaaactat gttgaaaatg ctgcaaaaat ctataaccgc
      301 aaaactggag caaatattga cttaaaaatt gtgaatgtag cttcgggaac agcaacagta
      361 caaatgatca ccccaaaatt ggtaacacac gaagatatgc cggatattat ctccatggca
      421 gacacttcag cagcaggtgt gctggaaaaa tttcaagatg ctttttatag tgctgatgaa
      481 tatggatttt atgacaaata tggcgatcag ttttatgatc aaaaattgaa tattttgcgt
      541 caacaaacga gtaatgaaac gattattcct tgggctagtg attttacccc agcactttgt
      601 tattatcaac caaaaaaatt tgctgaaata ggtgtagatt ttgaagaaat cagttcttgg
      661 gaagaatata tccaggtggc aaaagcatta aaagaaaaaa ctggtagttt tggaattgcc
      721 ttgccagaat ctggggacca agaattgttt atcaacatgc tagcccagca gaatcaatct
      781 ttgcttgatg aagaaggaaa tatccgtctg aatactaaag aagcgaaaca tgctgcaaag
      841 ttaatcaaag aaatgatcga ctcagatatt gtacatttct acggtgcaca agatgctgag
      901 aaagcatttc aagaatctgc aatgtttgtt gctggcggct ggtatgctac gaatatgtct
      961 ttgaattttc ctgatgcatc tgacaaatgg cgcatggctc cactgattcc tttttcaaaa
     1021 gaaaatccag gaaaaggtgc agtatcagga ggatcaagtt tttatgtccc taaagatgcc
     1081 aaaaatccac aagtggcaca acaatttctt acatttatgc tgacagatga agaatgctta
     1141 gccaacgcac tggagctggg cgtggctaca tctaactcat tggcctatca aacagaagca
     1201 gctgagaaaa agtttgacta ctaaggagga caaaaatact atgaactatt gaataaaatc
     1261 aatgaaaata catcaaatac tgttttacca gccacttatt cagatgctac gtcttatttg
     1321 actaccgctg catataacta ttggcaaaca ggtgattttg aaaattcata tctaaaagaa
     1381 gcagaaaatt ttgctcataa gtacggcgta aaattaactg gtgattaaaa aatattagga
     1441 ggacttaacg atgatgcaat cttcgttacc aagcaagaca ataaagaaca gcaagaaaaa
     1501 agcaacagat acgtctcata aaaaaagtgc ttatggattg gtcacacctt ttgttatttt
     1561 ctttattgtg acgattttga ttcccatcat tatctcgttt gcttttagtt ttatgaaaat
     1621 tggctactca tttgaatttg ttggatttga caattatcta gctatcttcc gagatccatt
     1681 gtttattaaa tcttacacga atgttttgat tatcatggca ggatcaattc caataacgat
     1741 gctattagct atttactttg ctgttttatt gaattcagca cagttaaaag gaaaaggatt
     1801 ttttagaact gtctattata ttccaaccat tacatcaacg gtggctgtag cttccgtatt
     1861 catgactttt ttcaatccga ctgggttatt caatggattt ttgaatgcgc taggaattaa
     1921 cggtgtacag tggttaacaa atcctttttg gatacgaata tcaatgatca tcacgatgat
     1981 ttggcttagt gtaggatata acactatttt atttttagct ggtcttcaag ggatttctaa
     2041 agagatatac gaaagtgctg aaatagatgg agcatcgaaa tttcggcaat tctttttatt
     2101 aacgatcccc atgctaaagc caatcatttt aatggccaca gttttagcca ctatcaatgg
     2161 cttgggtaat ttcaatatcc caaatatatt ctttgggact tccaatggac cagaaaattc
     2221 agctttggtc gtaggagtca atttatataa aacaagtttt gaaatggttg attttggaaa
     2281 agcctctgct attgcctgga cgatggtgtt tgtatctgtt atattatcgg ctattcaatt
     2341 taaattcgga ggtgataaaa atgactaaac aaaagataca aagcagaatt tttacgtacg
     2401 tcattattgt gtttggtgcc ttaatatttt tgatgccata tttatatatg attgtagctt
     2461 caacgcaaag taatcaagaa attttaagtg gcggaatcaa tttcaagttt ggggatatgc
     2521 tattaaaaaa ctggcaagcc atcatggcga gatataatta tcccttgatt atttggaatt
     2581 cgattgttat tgctgtaatc agtacagttt cagctacagc cagttctaca atggcgggtt
     2641 atgcattagc aaagtatcgt tttatgggga ataaactgtt gttcaataca gttatgctat
     2701 ctaggatggt gccttttttt accacattga ttcctttatt ttacattatg agtcgtttag
     2761 gattatcgaa tacttatgca ggtgtgatca tcccttcgat tgccagtaca acttcagtgt
     2821 ttatgatgcg acaatatagc agtcaatttc ctacagcact gatggaggct gccagaatcg
     2881 atggtgccaa tgagtgggtg atttttacga aaatcgctgt accgatttta tcacctacga
     2941 ttattactag tggtttactt atctttatgg gaagttggaa tcaatatcta tttccattag
     3001 tcatgctttc agataaagat aaatatacgg ttcccttaat tatcaaaaat atgacggttt
     3061 ctagtacagg tgagccaatt aattatggcg caattatgct aattttagca acttcagtga
     3121 tcccaatggt cttgatttat gtgtgggcgc agtctaaatt taaggagaat gacattggag
     3181 ctgcaaataa gtaaaaaaaa taacgaatgt taaccatcta gttcgttcta ggagcaactc
     3241 cacattcata aaaattaaaa gaatggcggg atatccatga atgaacagac gaaggaatgg
     3301 tcaagaaaaa ttttgaatgt agcttctgaa aagatagatg cggaattaga cagaatgggg
     3361 aataaaattc catatatttc ttacaacgga aaatatacag acgtaataaa ggacagagga
     3421 atcgactggt ggaccaatgg tttttggagt ggtattttat ggcagctctt tcacaagaca
     3481 aaacgagaaa agttcaaaga ggcagcaata caacaagaac aattattaga atctgccctg
     3541 taccattttt cagacgttca tcatgatgta ggttttttat tcttaccatc ggctattgca
     3601 cattatcaac agtctcaaaa caaacatagt cgacaactag ggttacatgc agctacggta
     3661 cttgctggac gctataatat ttcagggaac tatttagtag catggaatga ccataaacct
     3721 ggttggatga ttattgatag tatgatgaat ttaccattac tgtattgggc ttcgagggaa
     3781 actggtgatc cgaggttctc taaaattgct cggcagcatg cagatacaac agctaagtat
     3841 ctagtgagga aagatggatc aaccgggcat atcgcagaat ttgatgcaga atcagggaaa
     3901 tatattcggt tacttggcgg gcaaggatac aatgagaatt cttgttggag cagaggaaat
     3961 gcatgggctt tatatggatt cgcgttaagt tatcgttaca caaaaaatcc gtactatcta
     4021 gctatagcag aaaagatagc agtaaatttt attcagaaaa tcgaaaaaac tggttatatt
     4081 cctctcattg attttgctgt aaaagattcg aatattgata ccagtgctgg agtatgcgct
     4141 gcatgcggtc ttctggaatt agcaggatat gtttcctctg tacgatcaaa aatgtatcat
     4201 gaagctgcta tcaatatact ccaaaagaca gaaaagcact atgcaaattg gaatatggat
     4261 gaagatggga tcattggagg cggagcagaa gcataccata gagaactgac ttataatgtg
     4321 ccactgatct attctgatta tttttttatt gaaggcttac ttcgattaga acagtcggaa
     4381 ttagctattt ggtagtaaaa agaggagaaa atacaatgaa acatccagta ccactattaa
     4441 ggaccagaca ggattacttt gacttattta aaaagataaa tcaaccactc cagccgtttt
     4501 atcgagcaag tggcgcacaa attgattata tacatcaagg ggtaggatat ggaaataaaa
     4561 ttgcaggaat ggaaggattc tctcgggttt tatggggagc ggggcccgca attgaccaat
     4621 tagatgaacg atggttgaaa ttaattctat caggaataaa agctggaaca gatccttccc
     4681 atcccgatta ttggggagaa atacatgaaa gagaccagcg tatggtagaa atgcccgcaa
     4741 tgttattagc gttatatcat catgaacaat tattatggaa aaaattagat acaggagaaa
     4801 aaagccagat tattcattgg ttctcgcaaa tttttgaata tgaatgtgca gatggtaatt
     4861 ggcaattttt taagataatt gtcggaacaa ttatccaaaa atttggttat ccagttaaag
     4921 aatcagcact gaaagaagca tttgataata tagaaaattg ttatcttcaa aatggctggt
     4981 atcgagacag ttcaagaggt cgccaagatt attataatcc ttttgcattt cattattatg
     5041 gtttgattta tagcgtacta gaaccagaaa agcccattag ccagatatat aagaaccgag
     5101 ctattgattt ttctagagac tatattcatt tttttgcgga agatggtgca aatgtgcctt
     5161 ttggtcgaag catgatttat cgttttgcag tttcaagctt ttggtcagct atgttgtggg
     5221 ccgatctttt gccttttaaa ttaggtgaaa tcaaggggtt agtcaatcgg aatataagat
     5281 ggtggatgca aaaagaaata tttgatgaaa aaggtatcct gaatattggt tatacctacc
     5341 ctcaattgac attgactgaa ccctataata gtacgttatc tccattatgg ctgaataaga
     5401 tttttttatt gttggcgcta cctgaagacc atgagtattg gacggctaaa gaaatgccta
     5461 tgcctattcg tgaaggaact aaacttcttt cagaagccaa tttgctcata caacatgata
     5521 atggacatac attttttttg aatgcgggac aattagggcc aaatttccat acattgacca
     5581 atgaaaaata tttaaaattt gcttattcta gtcaatttgg tttttcgata ccgcgagcga
     5641 atcaattgaa agcagaaatg gcaatggatt caatgctagg gattcagcga gcggacacat
     5701 atatcacgac cagttttaag aatgagagca tcaaaaccta tggacagttc atcgttcgaa
     5761 ataatgtacg agacattgta tgtaagaaag aggtacttgc ctctacatgg gaacctactt
     5821 tacaggcaaa aatcagaact tggcttattc cttttgaagg ctggcagatt cgcgtacaca
     5881 aagtagaatt agatacagaa tatgttctat atgagacagg gtttgctatt gaatgttctc
     5941 ctgagaaaga aggaataatc atagaagaag acaaggaaaa ctatagggtg agtgaagctg
     6001 gcttttcagg tattatctgt ttaagtgacg atactattaa acgaaaatcc acagctatta
     6061 tgggtttgcc caatacaaac ttgatgacat gggaaaacac ttttattcct ggattagagg
     6121 gtacatttgg aaaaggaacc cattggctcg gaacaggagt ggttgctcat caagatcggg
     6181 attatagttt agaagtatgg aacaatcgtc ccaaaattga ttttgatggg acaaccgggc
     6241 taactatcca aaataaaaac aataaaaaaa gactaaaatt atgctaaagg ctggtgggga
     6301 aatgcttgat aaagtgatgt ttgaagagat aatacatcca gaaaaatgga taaatgaatg
     6361 ttataaagaa ttaatggaaa aagaatggaa taatgagatc gagcgttgtc gcagacacac
     6421 ggagaggtta atcaaacgta tcagtaacag tcagtatcaa ttcccaagcg agtgtcctat
     6481 aaaaggacgt tatccattaa gagacaatat tggttggaca gaaggatttt ggacaggaat
     6541 taattggttg ttatttgaga agacgggtga tcctttattt caacaaacag ctgaaaaagc
     6601 agatatagat tttatccaca ggattgactg tcaaattatc actaatcatc acgatcttgg
     6661 ttttttgtat tctttatcaa ccgtagcaga ttataaaata acgggaaatc gaaaaatgaa
     6721 ggaagcttca attgatgctg cagaaacatt acttcagcgt ttttgggaga aggcaggagt
     6781 tatacaagct tggggagatc ctagtgatcc ttcacaacaa ggtagaatga tcatggattg
     6841 ttgcatgaat cttccattat tgtattgggc gagtgaagaa actggaaatc aaaaatataa
     6901 aaaagctgct caggctcata ttcaaaatgc agcagattat ttagtaagag aagatggttc
     6961 aacttatcat acattttata tggatgtcaa agatggaaaa ccaagatatg gaagtacgca
     7021 tcaaggttat agtgataatt cctgttgggc tagaggccaa gcatggggga tttacggttt
     7081 tgcattaagt tatcgttatt tgaaagaaca aaaatatttt gatttaagca aaatattagc
     7141 caattatttt atgaatcggt tgccagaaga taccatttgt tattgggatt tgatctttga
     7201 tgattctagc agagaagaaa aagatacatc tagtatggtg atcatggttt gtggattatt
     7261 agaattaata gaatcaggtc tattagacaa aacagaaaag aacttatacc agtctgctgt
     7321 ttggaaaatt atgcgacaat tgaaagaaag atatgctaca acggaagaag aagatggctt
     7381 tttaacacag tctgtttata ataagcccaa aaataatggg gtaaatacac cttgtatttg
     7441 gggtgattac tattatttag aagctttagt gagaatgact agtaattggt cttcttattg
     7501 gtgaaaaagg taaatatgga atttttgttt gataaaataa aaaaagaaaa accaaaacag
     7561 catgaatggt ttctgttttg gttttttcgt taattttcat attcttctaa ttccaagctt
     7621 agttcttccc attccgtcaa ttttgtttct tgatcttgtc tagcagcttc taaatcttgg
     7681 ttcagctcat tgagttttac gtgatcttcc aggttttctg gcgcgaccat ttcttgttcc
     7741 aactgatcga tccattcatc gatccgcgcc atttcttctt caacctgagt aatttttcgt
     7801 tgaaggctac ggataagctt ttgctgttct ttgctttggt aataatcaga tttgttcgtg
     7861 ggagcaggag cagattctgg ctcatcttgt gccaacaatt cggcgatttc ttcttcttcc
     7921 tgctttttct ctaaataata atcatagtct cctagataga ctttgctgcc attttcagag
     7981 agttcgacga cttttgtcgc gatccgattg atgaaatatc ggtcatggga aacaaaaagc
     8041 agtgtgccgt cataatcgat caatgcattt tctaatactt ctttgttgtc aatatctaaa
     8101 tggtttgttg gttcatccaa aatcaaaaaa ttttctttgt tcatcgctaa cttggctaaa
     8161 gcaacccgtg ctttttcacc gccacttagc agagggattg gtttttctac atcttcgcca
     8221 gaaaataaga aagcacctaa agcacttcgg atctcttttt ctggagtggt aggatgctca
     8281 tcccacaatt ctgctaggat cgtcttgttt gtatgcagat cagcttgacc ttgatcgtaa
     8341 tagccgatgg tgacatttgt ccctagcgtt tcttttccac ggataaaagg gatctggtga
     8401 atgattgatt tcagcaacgt cgatttcccg ataccattcg gacctactag agcaatcgct
     8461 tcttgacgac ggatatctag attgattggt tcagataagg tgacattatc atatccgata
     8521 gcggcatctt cgatttgcaa gactacattt ccagaaggct tttctatact gaatagaaag
     8581 tttgcttgtt tttcatcccc ttgaggacga tccagccggt ccattttttc taatgtcttt
     8641 ctgcggcttt gcgctcgttt tgtggtagag gcgcgaacaa ggttccgagc aacgaagtct
     8701 tctaatttat ggatttctgt ttgctgcttt tcataagcct tccattcact tgctaactgt
     8761 tctgctttca aatctagata tttcgaatag ttacctttgt aatggcgcat ttttcttctg
     8821 cttaattcat agacttcatt gactacttta tctaaaaaat aacggtcatg ggaaacaatc
     8881 aataatgcac cagtatagct ttgcagataa ttctcaagcc aggaaagtgt ctcgatatct
     8941 aaatggttcg ttggttcgtc tagaatcaaa atgtcagggt gttgcaaaag catgcgagct
     9001 aaagctaaac gtgtgcgctg tccgcctgat aaagtatcga ttttctgatc ataaaatgaa
     9061 gaatcgaatt taaacccatg caatacggaa cgaatttcat tttcataacc atagccgttt
     9121 cgatctgaaa aatcgtgttg cagccggtcg tattctttta aataagaact ataagcaggt
     9181 gtgtctggcg taccttcgct aatgaaaatc tctagctcgc gcatccgctc ttccatttgt
     9241 ctaacttgtt caaaagcttt cagcatctcg ttccaaactg tttcattgga atcaagtcct
     9301 gtgtcctgag cgaggtatcc gagagtcgct gttttatttt tagcgatctt tccttcatca
     9361 ggtgcttcaa ttccagcaat gatctttaat aaagtcgatt ttccagcgcc atttctgcca
     9421 actaaagcga cacgagcgcc tgtactgatt tctaaatgta tgttttcaaa taagacttct
     9481 gcaccaaaat ggcgagcaat ctgattggct tgtaataata tcatggaatc ctcttttcta
     9541 ctttgtgaat tctcctcaaa taaaaaacta tttttcaatt agtagtctac cataaattcc
     9601 attatactgc gagatttgca gaaatgataa ataaatgaat aaagaataaa atttcacaat
     9661 atctgagcaa tggaattgat tttttcctat aaatactgct atgatggaga aatcaaagat
     9721 gtaattagac aatacgtgga ggtcatttcg tgaaagatca tattattcca aaggcgacag
     9781 ctaaaagact gcctttgtat tatcgctatt taaggatttt aaataatgct ggaaagaaaa
     9841 aagtgtcttc aactgaatta agtgaagccg ttcaagtaga tagtgcaacg atccgtcgag
     9901 atttttctta ttttggcgaa ttaggaaaac gtgggtatgg ttatgatgta gaagatctta
     9961 tgcacttttt tgcaaagaca ttaaacgacg atcaattgac aaatgttgcg ctgatcggtg
    10021 tagggaactt gggaagtgct ttactaaaat acaaattcca tcaaagtaat agcatacgta
    10081 tcagttgtgc ttttgatgtg aatgaggaaa tcgttgggcg catcgtagac ggtatccccg
    10141 tttatccaat ggaagatatg tcagaacaaa tcaagatcca acaaatcgaa gtagcaattt
    10201 taacgatacc cgctgaaaga gcacaagaag tagtcaatca gttaacggaa gcaggaatca
    10261 aaggaatctt gaactttacc gctgctcgat tgaatgctcc gtcagatgta ttgatccaaa
    10321 atgttgattt gacgaatgaa ttgcaaacct tgatctattt cttgcattct gacgcaaatg
    10381 cgcattccga aaatacagca gaataaaaat aaaaagcgat tgaacaaatg aaggcaaaac
    10441 tttcacttgt tcaatcgctt ttttctgttt ggacaacgat tattttgttc ggaagatttc
    10501 ttcataagaa agggaatcat cttctgctgt cagttcatct agtagataac ggattttttt
    10561 aaagtcgatg aaccgtgctt gccgaaagac ttcttttcct tgatgaaaaa tcaggacaac
    10621 tggcgcagtc agcacttcaa atttactagc tacttctggt gtttcaaatg catccaaatg
    10681 aagtgccgga atatcataat gtgtgagaag ttcctctagt cgtggacgaa cagcgtgaca
    10741 aacactgcaa ttaggcatcg aaagataaat gacggcaacg gatgactgtt gcagtatgtc
    10801 ttgaacttca gtcaagttag tggtttgttt catttttatt ctccctttta tgatagaagt
    10861 gatatgtatg agaccttaat ttaatgggaa taacagaaaa agtcaattga agtgctttgg
    10921 taaaaaataa gagttgtgta aaaatttaca gagcaacttg ttttttttga ataaaagcgt
    10981 atatttgcaa agcgaatgaa ctagaaataa aaaggaggaa actcatgttt tcttttattc
    11041 caaattcggt tttgcagatg agtacgattt tcttatcgat cgttattgaa gcattacctt
    11101 tcgtattatt aggctgtctt atctcaggcg ccttacaagt atttttgacc ccagaacgtg
    11161 tgaagagatg gcttcctgaa aatcgtatct tctctatttt tactggttcg atcttaggat
    11221 ttttctttcc ttcatgtgaa tgcggcatcg taccgatcgt tcatcaattt gttaaaaaag
    11281 gcgtaccagt tcacacagcg tttgctttta tgctgactgc tccgatcatc aatccaatcg
    11341 tgattttttc cacttttatc gcatttggca attcttggca gatggcaagc tggcgggtga
    11401 taggcagctt ttttgttgcc cttgtgattg gtgtctggct tgcctatttt caaaaagacg
    11461 ctgtattgaa agaaaaaatc acgtctaaag tagcagaaaa tcaccatcat catgtccatg
    11521 aagaagaaca aagtgagaag aatgaaccaa aaaatcagct gatccatcaa actgggcatg
    11581 tgttgacaca ttcaatcgac gaattttttg atacaggtcg ttatttgatc attggcggat
    11641 taatcgcagc tgcgatgcag acttatttac caactagaat tatgctaacg ctcggctcaa
    11701 caaagatttt agccattatc atcatgctgt tattggcctt tacgatgtct ctttgctcag
    11761 aagcagatgc gtttatagga tcttctttgt taagtctgtt cggcacagca cctgtagctg
    11821 cctttttggt ctttggccca atggtcgata tcaaaaatct actgatgatg aaacgatatt
    11881 ttacaggacg ctttatttta tcgctgatcg gactgattgc ccttagcgtt attggattca
    11941 cattatttat ttaggaggga aaatatgctg cggtttttga ttttatctgg gtatttcgaa
    12001 ttgatgatgt acttgcaagt ttctggcaaa ctcgatcaat atatcaatgt ccattatcgc
    12061 tacttagcta ttctttcaat gattttatct gctcttttgg ctttagttca gctgtatttg
    12121 tgggtcaaaa acggaaaagc agaagaaaag gcacacgagc acgatcatga ccatgggttg
    12181 tcaaaacctt atcaacgtgg aatcgcttat gtactgctag ctttgccagt aatcgtgggg
    12241 acgttgtttc caacagttag tctcgacact acgatcgtag aagccaaagg atttaatttc
    12301 ccagtaagta aagaatcagt aggggatccg gagatgcaga cacaatactt aaagccggat
    12361 accagtattt atttcaacaa atcagactac ttagaccaaa tgaatgagtt gaaagaaaag
    12421 tatggggata agcaaactat caagatcaca gatgaaaact atttagaagt tatggagcta
    12481 atttataatt accctagcga attcattggc aaaaaaatca gctatgaagg atttgtttac
    12541 caaacaccga acgatagcaa tacagatatc ttcttgttcc gattcggcat tatccattgt
    12601 gtggcagatt caggagtctt tggtttgttg acgcatatgc cggaaggagt aacggttaaa
    12661 aatgatgaat ggtataaaat agaaggaacc atccaatcag actattatca accattcaag
    12721 cgtgaaatcc catctgtagt tgttacaaac gctgagaaag tctctgctcc gaaaaatcag
    12781 tatgtgtatc gttcttttta aaagaacaaa ataaataaac atagctcaga cagctgacac
    12841 gcaaggacgt cggttgtctg gctttttttg catgatgtat tcatttgttt gatatttatt
    12901 ttagaagcag catcctgaaa gtatttgata tcggtttcaa aggtgttgga aaagattgtg
    12961 aagagaatta caatttattc ctaacatgta tgttgaaaag ttgtatgaaa tgattttgat
    13021 agtaaaatat cgagtggagg tactttttat ggtaacgcgt atatttattt tggatgatag
    13081 tttggttttt gaaaaaatgc tagtagaaat tttggaagaa tccagaattt tattaagtcc
    13141 ttggaaattt gagatcgttc agggattgaa cgttatacaa tttgttgaat tcattaaaaa
    13201 taatgaaatc tattcctcag atattttttt cctagacatc gatttgaacg cctattatag
    13261 cggtatcgaa ctaggcgaaa tgcttagaga aaagaatgaa aagtgtaaaa ttgtttatct
    13321 aacgtctcat ccgaataaag caataactgt tattaatcga aagattacgc ctagtggtta
    13381 tttgataaaa gaattcactt atgaaaaaaa taaaaaaagc atccaaagtt acttattgaa
    13441 agaaaaagaa aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa ctgtccgtta
    13501 tggtaatagc gatcactata tccattacga tgaaatcctt tatctaatga gcatgccagg
    13561 ttataaaaat aaaataggcc tagtattgaa agatgaagag attctcgtaa acggcacaat
    13621 taaaaaatat aaaaaatcac tcgaatatga gtatttatgt aaagatttaa aagcttatat
    13681 cattaatact gaaaacatta catctattag cagagtaaac gaaatagtaa catttgcaaa
    13741 tggagaacaa ctagagatgg gaaagagaag tattgataaa ctaaagaatt ttatgcaaga
    13801 aaagatagaa aatgaatcat tttaaagaaa acaaagactc atatcttacc tgttagaaga
    13861 gtgttttcct gtagaagaga acaacccaag tagcagccta gtcgtttcta tccttttttg
    13921 ttatgcactc tttttgacat ttactccacc aataacgaag ggtacaataa aaagtaataa
    13981 caaacaaaag gagtacagaa atgattgaat tcatcaatgt aagtaaaagt tacgggaaaa
    14041 aacaagcatt aaaagacttg tctctctcga taagacaagg ggaaattttt ggatttttag
    14101 gacataatgg tgccggaaaa tcaactacga ttaaaagttt agttagtatt attgaaccat
    14161 catcgggaac gattttagta gatggaatga aattgacgga aaatcgattg agtatcaaac
    14221 aaaaaatcgg ttatgtgcct gattccccag atattttttt acaattgacg gctggagaat
    14281 attgggactt gatttcagct gcctatgaat tagacacaca aaaaaaagaa aagcgtttag
    14341 cagaattgac agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt
    14401 cacatggaat gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt
    14461 gggtattgga tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa
    14521 tgatgaaggc tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata
    14581 ccgcacaaca gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg
    14641 gggcagttag tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga
    14701 aaatggctgg aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa
    14761 ttaattcgag ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa
    14821 ggaaaaaagg gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg
    14881 atttttttac tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt
    14941 ttctttactt attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc
    15001 atttataata tcttttttga aggaaatgac ctgcaagtat ttttgcctct gccttttgga
    15061 caagaacagg ttttttcagc aaagattttg gtagttgctt tgaccatcat tccttttgtt
    15121 tttccgcttc tagtcatgtt tattttaacc ggatggcgag ctgatctagg gattttgctt
    15181 actatcatta tctcaatcct tttgttttct ctatttttga ttattctttt ttgtctggct
    15241 agtttgattg tttttggttt ggctcgaaca gcagtgttta aaaaacatag aaaaacagtt
    15301 tctagtatct tgcttggcag ctcgatgctg atcgcatttg ctggaatcat gtggatgaat
    15361 catcaaacgg ggactattga aaatgagtta acagatcggc atccgatttc gatattattg
    15421 ccattctatg atgctgccag tcagccactt tctcaacaag gtttattggg ttggggaata
    15481 ctagctatca cggctctggg atttttcctt gctgtaaaac agctgatagt accaaagctt
    15541 acagagcaac tgactgattc aaacagtgag ttcaaaataa aaagaaccca taaaaagaac
    15601 caaaatgtgc atcagctttt atttagttac aatgctcaat tagtaaaaga acctaatttg
    15661 attatgcaag ttctttcttc ttccttgtta actccaatca tttttattct tgcttttgcc
    15721 ctaggaggcg aaataaaatt aactgattta gataataagt taatcggtgt tgttttcctg
    15781 gtagggatcg cattggcttt cttaacagtt aatcagactt cttttatcag caatcttatt
    15841 tctttggatc aagaaaattt tctgtttatc cgttctttgc caatctctat gaaccaatat
    15901 ttaaaagaaa agttcagatt cggatggata ttgcagagtt ttttaactgg tgggatcgca
    15961 ttattaagcg gactgtcttt tcagctgcct ttgtttttta tccctagctt gctatttggt
    16021 tctttattcg gctgctactt attgtcacta cgttatttcg ctagagatta tcggttgctg
    16081 ctgctgaact ggaccaatat taaccaatta tttaacagag gttcaggaag tttgggttta
    16141 gtcgcaacta tgatgggttc attgatcatc agtattattc ttatcgtctt atacggaatg
    16201 ttggctttgt tttatccatt ttggttagtg aatattcctg tgatcggttt agttctaata
    16261 ctttccgctc tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt tgaataaaga
    16321 agaggggccg tgacaaaaat catgtcgcag cccctttttt acagatagag ggtgttcaaa
    16381 agtcagtttc tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta tttttacaaa
    16441 acttttttta atactcataa ttacttctta ttgctcctgc ggttacttgt cgcaatgttt
    16501 gtgagatatt ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt tctttgttac
    16561 acttcgttta tttaaaaagc cgtttttttc gtttcatcat gattgcgtca cgctttactt
    16621 tgaaattacg gatgttatgg ttaaagactt ctgataatac aagacctaaa gcgattgctc
    16681 cagcaatcat aattgcttgg accgcataat gagccgcttc tcgataggat tcagtaacga
    16741 gatttcgaac cgcttgataa gctaaaccac cagggactag aggaaccatc ccgggaatat
    16801 tgaaaatcgt tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg aatgctactc
    16861 ctaaagaacc aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca cagatccagt
    16921 agatcatcca gccaaaagca ccagaaagac cacaacaaat caatgaacgc ctaggtacat
    16981 ttgtaataac ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga ataaatatcg
    17041 tccccatact tgtgttcctt tcctaataaa aaagctggaa aataaaagca atcgcaaaac
    17101 cgatcatcgt agcagtcatc atcgcttctg ttccgcgaga aactcctgag acataatgtc
    17161 cagctagcaa atcacggata gcgttcgtaa tttgaactcc aggaactaaa ggcatcacac
    17221 atccgataat gattagatcc tgattgcttc ctaagccaaa acgtacgctg tagatagcgg
    17281 ctatgccaat caataaagaa gataaaaatt ccgataaaaa tttgattcta aaaaatttca
    17341 aactatatag ataagctgcg tatcccaatc ctccaatgac taaagtcaca ggtaaatcgg
    17401 tcatctttcc gccaaataac accataattg ttccactgat cactgccgcg ctaacgattt
    17461 gcaaccagac aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg cagtaaagtt
    17521 cttctaatgt tagctctgaa gcaacatatt tacgtgaaag atcatttact tttactactt
    17581 tttctaaatt aatcgaacgg ttggtaattt gttccattcg gatggtcgag gtacgatcaa
    17641 gaccaataaa gagaccggtt tgagtgacat agctgaccaa acggaaattt ccagaagcta
    17701 aagcaattcg actcatcgta tcttctaccc gatacatctc cgcattgctt tccatcatga
    17761 ttttcccagc gagtaaacaa gtgtccagca acagatctaa ttcttttccc gtcatagcat
    17821 cttctccaat aattgattga ttagttttat tttactacaa aacatatcgg aagcgtaaag
    17881 aataaaaaaa cttgaggaag gagtgaaaga ggcgtatact gtttttgtat cgcgggtgta
    17941 gtttagtggt aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc ccgtcacccg
    18001 cttaaaagag gctgtgaaaa agctgccctg catcaagtaa taagaatagt caagcaaaaa
    18061 agcttcttat gtttttgact tggttggact tattaccgca gatgcaagct tttgaacact
    18121 gtttaccaag aggtcgtaca gaagccgttt agttttgagt attaagaaaa attttttgaa
    18181 tgataataaa taccttgtaa atagtgtttt gtgattgagt agagacgatg aatccgtctc
    18241 tttttttgta ttcttaagcc gtctaaaaac tggttttatt tttttactgt ttctatttct
    18301 ttccagtatt tatttatagg ggaacatatg ttaagattta gggaactgtt agtaaaggag
    18361 taaagaaatg agcaaaaaaa caaccaaggc gatcgtgggg atcgtcgctg ctgtgattgc
    18421 aggggcactc ggtatttcca ccacacaaaa agattcagat atcttccaac aaattacaac
    18481 catttttgat tccacaccgc aagcttctca agtagcggat gtgccagaat atgatggaaa
    18541 acatcaagaa atagaaatca atgggaatca gccggagttt acaaaagagg atcttagtct
    18601 ttccaaaggg acatgggaaa gctattcaaa tatagacaaa ttgaacagag taggacaagc
    18661 caatgcgatg ttgggaaaag acttgtttcc tactgaaaag agagaacctt tgtatattga
    18721 tccgacaggt tggaaacaaa agaaattaag tgacggacaa tggttataca accgcagcca
    18781 tttgattggt ttccagttga ctggtcaaaa taataacatt aaaaacttaa tgacagggac
    18841 acgttcgttg aatgctccgt acatgcttgc ccacgaaaat gatattgcgg catatatcaa
    18901 ggaaacaaat catcatgtac gctatcgtgt cactcctcat ttcgagggaa atgaactggt
    18961 agcaagaggt gtgcagttgg aagcagaaag cattgaagac aaaaaaattg aattcaatgt
    19021 ctttatctac aatgttcaag atggctacac catcaattat gagacagggc aagcaacgaa
    19081 aacgaaaaat tagtagcagc aattcgaatt ttttatgaac tttatgaaaa aattgtgtaa
    19141 aaacatactt tcaaagaaat atagcgtata ctttatggaa agagggcttt tgaaaaagcc
    19201 acagaaaaaa ggaaaaatca gacaaatcgt gtttcttaat ttattcagaa gtttggaata
    19261 tttgtttgta gttttccctt tcttcgagta aaataagggt gtagaaggta agctggaatt
    19321 accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta cgttttggtt
    19381 ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat cgggctttgg
    19441 aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct ggagactcgg
    19501 cctacattcc tcgttatcgt gatgcacgaa atttgattcg ccgtcacgaa cgcgatgaaa
    19561 tcatggaagt aattgtcaaa gactacctct caaatcatgg agtgaattta tgagagtaat
    19621 gggcttggat gtcggttcta agacagtagg cgttgctgtg agcgatccct ttggctggac
    19681 agctcaagga gtagaaatca ttcgaatcaa tgaggcagaa gaagagtttg gttttgatcg
    19741 gttgaaggaa ttagtggcgc agtacgaagt agaccgtttt gtagttggat tacctaagaa
    19801 tatgaacaat tccatcggac caagggcaga agcttccatg tcatatggag agaagatcaa
    19861 agaactattc tccttgccgg ttgattatca agatgaacga ctaaccactg ttcaggcaga
    19921 acgtatgcta gtagagcagg caaatacttc aagagcgaaa cgaaaaaaag tcatcgataa
    19981 attagcagct gtgatgattt tacaaaatta tttagacggc tcaggtaagc aattatttta
    20041 ataataaaaa gaaaagaggt aatcttatga cagaacataa ccattcccac gaacacgaag
    20101 gacacgaaca catcacattg gtggatgacc aaggaaatga aacattatac gaaatcttgc
    20161 taacagttga cggacaagaa gaatttggac gtaactatgt tcttttatat ccagctggtg
    20221 tttcagaaga tgaagatgta gaattacaag cttatgcata catcgaaaac gaagatggaa
    20281 cagaaggcga gttagaacaa atcgaaacag acgcagaatg ggatatgatc gaagaagtat
    20341 tcaatacatt catggcggaa gaagaataga acaaaagaat cgataaggtg taagaaaagc
    20401 tggactctct gaaaagtaga gcccggtttt ttcgtttagc actaaatttc cttaaaaagt
    20461 agaaaagctt tcgaagcctc gtttgtttct tttactgtat aataaaagaa aagagtaaca
    20521 atcgctaaaa gatataaaca agagaaagga aatacaagat gattggtctg atttattttg
    20581 tggtgatcgt tctggcgaac acagttggcg ctatttccgg gatgggtgga ggcgtgttga
    20641 tcaaaccgat tcttgatctt atcggcgccc attctgttgc agggatttct ttttactcaa
    20701 cagttgcggt attcacgatg tccattgttt cgactgtgcg acaagtatca agcgggaaat
    20761 cactgaattg gcagattgtc ggttgggtat ctagtggagc tgttgttgga ggagtagtcg
    20821 gcaacattgt attcgaagtt tttttacagt tatttgaaaa cgagaagcat gtgcagctga
    20881 tccaaatttt tttgacggta ttgaccttgg tttttgcgtt cttctacacc aagcatcatc
    20941 agccaaaatt tcatttgacg tcatggactt ggtaccttat atgtgggggg gttctgggct
    21001 ttttagctag ttttttaggt attggtggtg gaccagtcaa tgtttctttg ttgatgctga
    21061 tgtttgcatt gccaataaaa gaagcaacac tatattctct atctacgatt ttcttttctc
    21121 aactggcaaa attggtaacg attgctttaa caagtagctt tatgcgcttt gatttatcta
    21181 tgttgtttta tgttatcccc gctgctgtta ttggaggttt gtggggagca agatttagca
    21241 gggttttgtc tccaaaaaaa gtgaccttta tttttcaagc gattgtcatc gtggttcttt
    21301 tgatcaatct ttacaatgct ttcgtcatct tgcaaacatt agaaggatct ggattcaagc
    21361 aagtcaaaat cactgaaaag tcttttttta agtgaaaaaa ggagtatact gtgataaaaa
    21421 aattcattta aaaaaataaa aattggctgg tgatttggat gttgcgggaa atgcgtttag
    21481 gagatgcaga agcactacga gaaatcaatg cggaacaatt aggatatgat gtaccattaa
    21541 ccgttacggt gagacaaatg aagaaactgt tgaaggagcc agaaaaaaat tttttcttgg
    21601 tctatgaaga tcctacttct aaaaaagcag ctggctatgt acaagcggag tcctatgaat
    21661 cgattttcag cgatccgatg ttcaatatta tggcacttgc tgtgtctaaa catgctgaaa
    21721 aaagaggaat cggaaaggcc ttgatgactg gcgtagaaga agcgaaaaga cgaggaatat
    21781 cagcaataag acttaattct gcagaataca gagtagaagc acaccgattt tatgaacgca
    21841 tcggttatca ttccgataaa atgcaaaaaa gatttttgaa aatcctgtaa atttttatca
    21901 aaaaatacct ggtcatcaag ccgatacttg atgatcaggt attttttttg actaagaaag
    21961 taattgcatc aagaaacctg agatgatcaa aacgatggca ccaccaagcc gattgcccat
    22021 ttgagcaaaa gcaataagtt ccatccgatt agctgcagat aagacagcta catttcctgt
    22081 accacccatc gagttgttgc ataagccagc agtaatagtt gattcgattg gatataaacc
    22141 aaacaaacga ccgacaaatc ctgaaactac tgaaatcgcg ataacacttg tcaaacataa
    22201 gacaacaaat tgccatgtca aagcagaagc caatacattt agattcaata acgcaatacc
    22261 gataccggca agaacagctg ctgtcaagtt tttgacaatc aagttgttga acatgatagc
    22321 tgcatcttcg taatattctg gtaataggtt tgcgatttta cagataacaa cgataatgat
    22381 catgaatgcg taagcatgga ctttaggaac aaaatagtta cagatttgtc ctaagatgaa
    22441 gaaagtcaat gataccatca aaccgacacc taattgtgga atggaaggtt ttacatttct
    22501 aggttttcca tcgccaaggt cgcctttttc cattaattta ccattaccgt tgaattttgg
    22561 cattgattgt cccgcacgag caactaacgc tgcaccaatg atagctagga catttgtcat
    22621 tgctgtcgca ggaatcaggc gggaaatgat agaagaagcg tccgtaccta aaacatttgc
    22681 ataaatcgtt gataaaggta cagctccggc tccaacacca ccagccattg cgggcaatga
    22741 gatatataaa acggaatttg caaaaccatt tccgataagc atacctacga gtcctacggc
    22801 aaaaaaggag acgaccattg aaataagtgc aacgggaata aaacgaacag aagctttcat
    22861 caatagatta cggttcatac caaggatgct accagtaatc aaggcagcaa tataaaagtc
    22921 taaaaagccc atattgctga caaagttttc tgtggatttt actacatact caggtaagat
    22981 cccaaatgta gcaatcgcgg cagaggcaaa gatcgcaaaa acggagccac cacctaaata
    23041 acttttgact atagggattt ttgttccgat aaaatgaaat aaattaccga aaatgaccaa
    23101 aacagcgatt gggccaatca tattcgtcgg taattttcct aaagccattg caatgatcag
    23161 gacaaggacc atgatcaaat aaaggggcaa acttacgccg gtaatttttg ttgaccaaaa
    23221 cccttctttt ttcttttcct caactgcagt tgcggttgtt gttttttgtg ccatttttac
    23281 agatcctttc ttattctgta tcgtatgaaa aaagccaaag agaagcggca aaagagcagt
    23341 agttggctgg ctgctgtttt gccgcagtcg cttttttctt tttagacgtc atacaaagtt
    23401 tctgtatgtt cttttactat aacaatctta atcttctaaa gatttatatt caggaaccca
    23461 tttagcagat tcaacagctt ctttgatatc cgtgatttct tcacgattca atttttgtgc
    23521 aacaactgat tttgcgacag tttcagcaat cgtttgagag aattcagtga tttttgctac
    23581 tggaggcaag atagctgctc ctggttttgt tacatcaacg attccgccta atgctcgact
    23641 agcttgagaa atgatttcag catttactcg tgtagctgtt gaagcaatca aaccaagccc
    23701 taaacctggg tacatcaacg cattatttgc ttgaccgatt tggtaagtta caccgttata
    23761 ttccacatca gcagcaggga tacctgttcc aactaaagct tttccgtccg tccattcaat
    23821 caaatctttt gcttttgctt ctgccaattt tgttggatta gacaaaggca tgatgattgg
    23881 acgtggggta tgagccgcca tttctttcac gatttcttca gtgaatgctc caggttgggt
    23941 agaagtaccg atcatgatcg ttggatggat tgcttttaca actgcttcta agtttgtcaa
    24001 ttcatcagca ttagtgaatt ctgagcgttt acgagcgaat gggatttgac caggagtcaa
    24061 tccttttgta tcttcaaaca ataaaccctg tttatctact tgatagaaat gttggcgtgc
    24121 ttcttcttca ggtacacctt gacggatcat ttcatctaaa aggatgtttg cgatacctac
    24181 accagcagta ccagcaccaa atgtcaagat cgtttgatct ttcaattgct cacctgatat
    24241 attcaatcca cctaatacac cagctagtac aacgattcct gtgccttgga tatcatcatt
    24301 gaaagtagtg attttatctt cgtatttttc taagatcgtt gcagcatttc ctcgtccaaa
    24361 atcttcccaa tgtaataaca attctgggaa taattcagta gctgattgaa cgaattgatc
    24421 aatgaattcg tagtaagttt cgccttctac acgagcatga cgattcccta agtataatgg
    24481 attattcaat aattcttcgt tattagtacc agcgtcgatt gatacaggca agacttgtgc
    24541 aggattgata ccagccgcgg cagtgtagac catcaattta ccgatagcga tgtcaacacc
    24601 gttcacaccc cagtcaccca tacctaagat tccttctgca tctgtcacaa cgatcaaacg
    24661 aatgtcgcgt ccatctgctg cgtttttcaa acttgctttg atattttctg gttcgtcgat
    24721 tgaaagaaat acagcatctt gtggtttcaa gaaaatttcg ttgtattgtt cgattgaatc
    24781 cgcaacgaca ggatcataaa cgacaggcat gaattcaact aaatgttcgc ccatcaattt
    24841 ataaaacaat gtccggttcg tattgaatag attcattaag aagatacgtt tttccaaatc
    24901 ggtctgtttg cttaagtatt gaccgtaagc ttggacagat tgttgttcca atgtttggac
    24961 agtacttgga agcataccag tgattccgta ttttgcccgt tcttcttttg taaaagctgt
    25021 tcctttattt agaaaaggat tatttaataa gtttagccct gtaagcatga atgattcctc
    25081 ctaagtttta atacaaacga agtataggag tcagatgagg atatagtcaa aaagagcagg
    25141 tataataaat tttatttata tgagtaatag agtcaaaaga aatcaaaata gcggtatttg
    25201 ttctttttat atcaacaatc cttgcttgat tactatgcgt atctcttata tgctaaagaa
    25261 agaaaagtag aagaataaag gatatgttag atagataaat agaatcctga ataattcata
    25321 gctttaattc tttggcacat tttattgaaa tatgtatcac taatatccct atcaactgat
    25381 tttgactaaa aagttacttc aaacattcac tgttcaaaaa tattgaggaa taaattttgg
    25441 gtgtaaggca ttttgagcat acttctccct ggataacttc ccagtaaaaa aatcgttaga
    25501 ttgttggatt agatccattg actggctcgc ctcaggcgag caaagaccct gtagaattca
    25561 ttctgataca catgataact agataatttg agatagactc gtctacccgt ttggactaat
    25621 ttcccagcaa ctttcagaaa cttcaatcga atagaatgaa tggtcattcc cttttggact
    25681 tcttcaaagc caatcgttct taagaagttg actaagttgt aagctatcaa gctgagcatc
    25741 attcggacat gattctccaa aaagcgagga ctgtctgttt tgtcaaagta aaagccagct
    25801 ttcgcttctt taatgaagtt ctccattgtg ccacgtttgg catagataga aaagatggta
    25861 tcaggagaaa cattttctga tagattcgtc acgataaatt catgtcgaaa gagtagttcg
    25921 cccgcttcac gtgttgagcg tatacacacg cgacgacttt gtgaccagga ttgtgcttga
    25981 taagtggtcg agaagtactg aacttctcgt tcttcccact tttgattatc gccataaagt
    26041 actgatttct cagctatttg acctagtcta cgattattct tcagtcgaat aacataatga
    26101 ctcttttttg attcacacga atcatacaca ccaggtgtag cgaacccgct gtctccacga
    26161 accaagatgt cagtgtttgg tagagagtga ttatagtgct ctaataaagg tgtgagaaac
    26221 tcctttacgc cttttgatgt atattgattt cctgaacgta gttcagcttt taagaagtca
    26281 ccagtcaatc cgtcaaaagc taccaatgga tggtaaccat aggtttggta gtgtgtatta
    26341 taattcgttt gttcttggtg accaaatgta tctgaatggg tcgaatctaa atcaatgatt
    26401 aattccgtat cattacggat gaggcgtgct ttatcgataa gttcttggtt caaagcttga
    26461 agttcatgga tattctcctc agatagtcga tctaaaaatc gggaaagtga agattgagaa
    26521 gcgagttctt tcctatctaa aacagcttta aacacaggat cttgtctcag gagattagct
    26581 gcagaatcag ctgagtaacc agcaattaat tgcataatga actgctcaag tatggataag
    26641 ttgtcatgag taaaatacgc tcgttcgtct tcaatgtgaa tgtgttgctt agccagttca
    26701 gaaaaaccaa agatgttcat cagctctttc actaggacga gacccgaatc actcgataat
    26761 tgaccacctg tatgagaaat agtgatattt gaattgaatt ttacttggtt tttgtgtaag
    26821 ctagtcatga aaagaactcc tttcttttgg ttggtttagt cactttaacc atagcagaaa
    26881 ggggttcttt tttcatcact taacgggtga agatgaaaaa gtaattgtaa gctgccgtga
    26941 ggcagtttca catggtttta attaaaagta tgaattattc aggtagaatc tctgaagaat
    27001 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
    27061 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
    27121 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
    27181 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
    27241 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
    27301 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
    27361 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
    27421 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
    27481 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
    27541 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
    27601 aaaatcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
    27661 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
    27721 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
    27781 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
    27841 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    27901 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
    27961 ctcatgtatt aagagatgta gaattacccg atacaactac acattttgac aaagaaacgc
    28021 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
    28081 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    28141 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    28201 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
    28261 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    28321 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
    28381 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
    28441 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
    28501 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
    28561 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
    28621 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
    28681 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
    28741 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    28801 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    28861 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
    28921 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    28981 taaaattgaa atattttttc atacttcgcc gattgtaaaa ttaagctaga caactaaaaa
    29041 aagtcatttg tgctacactc aaaatagttc cgacgaaaga attataagga gtgagtacaa
    29101 atgacctata accatcttac accaactgaa ctcgtaatga tagaagctta tttcaatcaa
    29161 tcacaacctg tttcaaaagt agccaatctg ttacaacgtt ctcgacaaac tatttataag
    29221 gtctatcgtt ttcttaaatc tggtggaagc gcaatgagct actataaaca atataagaaa
    29281 aacaaacgta actgcggaag acatccgatt gttttacctg atgagcaaca ggaatacatt
    29341 caaaaaaagg ttgttcaagg gtgggctcct gatgtcctta ttggtcgagc agagtttcct
    29401 attgcttgtt cggtacgcac actgtatcga atgtttaaag ataaaacctt tgacactcac
    29461 gatttaccaa tgaagggaaa aagaaaacca aacggtcata aagaaaaaag aggaaaacaa
    29521 gggtttcgcc ggtcaatccg agacagaaag acagactata cccagtttga tcatgaattt
    29581 ggtcacctgg aaggtgacac tatcgtgggt ggaaaacata aaagcgcggt catcacatta
    29641 gttgaacggc tatctaaagt catcattacc ttgaaacccg aaggcagaca ggctaaagac
    29701 attgaaaata ggctgaatca atggtttcgt tgtgtcccaa aaaacttatt taaatccatc
    29761 acatttgatt gtggaaaaga attctcaaat tggaaaaaga ttagtaatac caacgatatt
    29821 tcaatttatt ttgcggatcc aggaacacct tctcaacgag gattgaatga acattcaaat
    29881 ggcttattac gtaaagatgg tttgtataaa gctatggatt ttaatggtgt atctgaaacc
    29941 tttatccaat cggtggcatc gaaacgaaat catattccta gaaaatcatt acactatcgg
    30001 acaccaatgg aagtattttt gagttacgtc acaaaagatg atttgtctaa cttaatttga
    30061 caaacgaaac ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct
    30121 cttttataca aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc
    30181 atactgtctc tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa
    30241 catgattggg acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt
    30301 tgctgagggt caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt
    30361 ggagcctctt aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc
    30421 agcaactggc cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac
    30481 acacgagatt gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc
    30541 ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa
    30601 cgtgacagtt tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt
    30661 agattttgga acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag
    30721 tcaaactgta actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa
    30781 agatcaaggt ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt
    30841 gccgaaagca gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc
    30901 agtagcatag agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga
    30961 ttttttaccg cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg
    31021 tttgtttcgt ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca
    31081 aaggattcag caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa
    31141 gcaatcggtt attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt
    31201 atcagcaatt cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg
    31261 agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg
    31321 tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca
    31381 gaaacaactg tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta
    31441 gcgggtattc atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca
    31501 caaatcaaaa atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg
    31561 atagaaccgg atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact
    31621 gttcagcttc gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc
    31681 aagatctttt atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca
    31741 ccaaattcga attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat
    31801 taccgcgcag aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac
    31861 tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa
    31921 gaacaatctt tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg
    31981 attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt
    32041 gatttcgttg aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag
    32101 ctggcaagag ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct
    32161 aagtccaatt ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct
    32221 accgatttat aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat
    32281 ccgttttctt tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg
    32341 gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct
    32401 ccttcttttt tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag
    32461 ctgttggagc aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac
    32521 ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta
    32581 accaattgcc taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag
    32641 gtggcacggt ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg
    32701 tgaatatcac tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat
    32761 caagcagtga tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg
    32821 cccccatcaa ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg
    32881 ggatggggtc gtgggataca aatactctat tgacagatat tattgctatt attacgcgag
    32941 gcgaattcaa cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga
    33001 tgacagaaaa cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat
    33061 tacaaccttc tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata
    33121 ttatccaaga tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta
    33181 aattaactgg cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa
    33241 atacgtggaa tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg
    33301 ataccacaga atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga
    33361 cattgacgat ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc
    33421 gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc
    33481 cagcaagcac aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc
    33541 aaggcggatt gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa
    33601 cggttttacc aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat
    33661 cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca
    33721 ggctttattc gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa
    33781 cgatccaatc aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt
    33841 cagggcaata ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt
    33901 cttttgcgta caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag
    33961 tggctaattt tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg
    34021 agacaggaga tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca
    34081 cgttaacaaa atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa
    34141 atactactta tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca
    34201 ttcaacaagg agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc
    34261 ttacttacaa ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga
    34321 caaaggcagc tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc
    34381 cagttgtcca aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta
    34441 atgtaccaga tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt
    34501 atgaatcaaa aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc
    34561 cttggcaact gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa
    34621 gcggaacaaa attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa
    34681 acacgttgat ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg
    34741 ataccgatgg tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt
    34801 atcaaggcat ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt
    34861 gtatggatct gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg
    34921 ctgttaaaat cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa
    34981 aatgaacaac ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc
    35041 gttaataacg tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt
    35101 tatttacaag ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat
    35161 attttattaa tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg
    35221 cgctattcta caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg
    35281 cagaaacttg tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa
    35341 ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca
    35401 gaaagtcaaa ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa
    35461 aacaaaaaag aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag
    35521 tttagttttg ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg
    35581 aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga
    35641 ttgtttcaaa cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt
    35701 caatatgcct ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt
    35761 aactttacat tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac
    35821 aaagacgacg tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc
    35881 tctttttatg tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat
    35941 cacgctcatt ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa
    36001 cagcggttaa ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga
    36061 caaacatttg ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta
    36121 tttgctcgct atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa
    36181 attgcttatg atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag
    36241 gaacaattgc tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat
    36301 gatatcattt tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt
    36361 gtattcacta gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa
    36421 tgttatttag aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg
    36481 cttcccatga aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag
    36541 ttttactgat cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc
    36601 tttatttaaa aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc
    36661 aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac
    36721 atcagctcat cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa
    36781 tcgtagcgac aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct
    36841 acacctataa aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag
    36901 aagtagtgat cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata
    36961 aaggatacat tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata
    37021 aggatatgat caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa
    37081 atgaattctt gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt
    37141 ctcaaacaat aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat
    37201 tgttaaaaga aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc
    37261 agacaagtga tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa
    37321 ataaacggca tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag
    37381 cgctaacttt gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag
    37441 tcattataga ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg
    37501 aacaaaatac cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa
    37561 acaaaacagt aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac
    37621 agctgataag ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac
    37681 acaaaactac gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct
    37741 ctcgttttca ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca
    37801 atagagtaga taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata
    37861 aatattaatc aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag
    37921 cataatattc gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag
    37981 gaggaggagt taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc
    38041 gttagttgca ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc
    38101 ttatggctta gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt
    38161 gctttcgaaa gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga
    38221 agaagtcgaa ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa
    38281 agacagtaca gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt
    38341 catcatgccc aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga
    38401 tattagtttg aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag
    38461 tgacaagacg tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt
    38521 tctctataca accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga
    38581 aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt
    38641 agaacagatc aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt
    38701 gacttttgga tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt
    38761 catttatgta ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat
    38821 cacaggagga tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg
    38881 tattcttctt ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc
    38941 attccaaagc aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt
    39001 gttaggcgca ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg
    39061 ataagaagga ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa
    39121 gatggcgacc aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa
    39181 tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc
    39241 ggattgcaga cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa
    39301 aaagaaagcg aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc
    39361 aatctggttc cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat
    39421 gaacatcgtc aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc
    39481 aacaagtatc cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca
    39541 ttgtacaatg atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa
    39601 aaggcacgag aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg
    39661 atcatggtta ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa
    39721 gatggtgtat tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata
    39781 caacaaagat acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg
    39841 atatctcggc acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct
    39901 ctatcattat ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa
    39961 agatgttgga aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac
    40021 cttgacaaaa atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct
    40081 tcacgatctt gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa
    40141 gaaaaattat cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa
    40201 tgggatttca aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac
    40261 tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt
    40321 gaatggtgta ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga
    40381 agcaagatat gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat
    40441 acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc
    40501 caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat
    40561 ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat
    40621 gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta cagccccttt
    40681 tttgcttact taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt
    40741 caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt
    40801 ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca
    40861 gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa
    40921 tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta
    40981 taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga
    41041 caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata
    41101 tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg
    41161 gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa
    41221 gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc
    41281 atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg
    41341 cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa
    41401 tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct
    41461 tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag
    41521 aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa
    41581 aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga
    41641 ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg
    41701 tttggccggg cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt
    41761 gagcctgccg ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt
    41821 gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt
    41881 tgtgaaatca acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata
    41941 ttttgccgtt tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac
    42001 aagggactgt gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt
    42061 tttctgtatt ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct
    42121 ccaatagttt tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc
    42181 cagcttcagc tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag
    42241 cagcagcaac agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta
    42301 attgattttt ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga
    42361 tcaaaatcgc ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat
    42421 tgtgcaaagc gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt
    42481 ctggagataa gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga
    42541 tgatttggct atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa
    42601 gctgatcacg tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc
    42661 cgacctcatc actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg
    42721 tttttgctgg cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg
    42781 cattcgtacc ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt
    42841 tttttgcagc aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg
    42901 ctttcatttg actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct
    42961 cctcgttttt atacataaaa ctataccgca actcacgact tttcgttttc taagagattg
    43021 cgagacatag cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg
    43081 tggagaacgg tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga
    43141 ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac
    43201 atatactatt atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa
    43261 tgaacagtta gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat
    43321 cttgatggct gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct
    43381 ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg
    43441 tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca
    43501 agcagatcat accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa
    43561 aggacaaatt aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta
    43621 aaaaagttag aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct
    43681 aattgcgttt ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg
    43741 atatgtattg tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga
    43801 attatacatt ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt
    43861 atttgccttt cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct
    43921 aattggctgg ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc
    43981 gatcttgaga caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt
    44041 tactatattt ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca
    44101 attcggcgag catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa
    44161 gatgtatgag ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt
    44221 tcgttttttg tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct
    44281 cttttttcat agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga
    44341 atttgaaaaa gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga
    44401 actggcagaa ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac
    44461 tgaggatgga ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa
    44521 tatccgtccc catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc
    44581 acaagtttcc agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc
    44641 tgacagtgaa atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc
    44701 tgttttgagc cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc
    44761 ttctcctgag ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga
    44821 acagttagat gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac
    44881 catgcgaaat gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg
    44941 tgtagtccac gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt
    45001 ggatttgaat aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat
    45061 tttaagtgaa ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta
    45121 tgtgattggt aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc
    45181 catcgtgccg ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact
    45241 gatcgatcag gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat
    45301 cgtcattaca ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt
    45361 acaactgatg gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat
    45421 ttttgaggaa gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac
    45481 cttttcttct catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct
    45541 ggtattattt gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat
    45601 cgcaatctta gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc
    45661 tgaattgaaa gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga
    45721 tgtggataca ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc
    45781 gtttgaaatc tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt
    45841 gatgaatgat gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa
    45901 aatggcagag accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca
    45961 cgatgatttg aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa
    46021 agcaaagaaa aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat
    46081 tgctgatatt cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt
    46141 gatcgatgca aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa
    46201 agtattgaag aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac
    46261 gacttacggc caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat
    46321 cggtatatta aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga
    46381 gccgacacaa cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca
    46441 attggacctt cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga
    46501 ttctgccatt ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc
    46561 acttcgcaag ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt
    46621 tgcaccggca aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact
    46681 aaaggtaagc agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta
    46741 aaaatggagg tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca
    46801 gataaaggac ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa
    46861 gcaccgattt tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa
    46921 atggatgtgg atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca
    46981 ttaatgttga aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa
    47041 caactacgtc aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg
    47101 tgggactgaa tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt
    47161 aatatttttt gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat
    47221 aataacttta attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt
    47281 agctttactt cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat
    47341 ttatgtcggc aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca
    47401 taatacgaaa acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga
    47461 atcgaatatc gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata
    47521 caacattatg ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata
    47581 tccccgtttg gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata
    47641 tcccgatgtc ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag
    47701 aaaatgcaaa cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct
    47761 gtgtccttat tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa
    47821 aaacttttta aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa
    47881 agctgctgaa aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat
    47941 tgagacaatc atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt
    48001 tggttatgcg attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa
    48061 gctaattgaa cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt
    48121 gacttacctc ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat
    48181 tcctgatacg atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc
    48241 aaaacgagga gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt
    48301 gaatgaacgg tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg
    48361 tttaggaaac gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa
    48421 tatcatgggg acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag
    48481 aaaagaatat cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat
    48541 gagagaagtc atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga
    48601 tttgatcttg atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa
    48661 tcaattagga atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa
    48721 tgagttgcta ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt
    48781 tttcttactc cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca
    48841 gctacgcagt aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa
    48901 acgaaaaaaa atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac
    48961 agaagaatta caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt
    49021 acatcaggaa tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt
    49081 acaacaaaaa gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct
    49141 aaggatatat gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt
    49201 taaaactttt gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa
    49261 aatcatgggg ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt
    49321 ggattttttg acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa
    49381 agattatgat gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat
    49441 tcaagaccaa ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc
    49501 aaaaatcgac ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa
    49561 atctttgtct aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc
    49621 taaattgatc attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct
    49681 aaaagatgga atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa
    49741 tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt
    49801 tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga
    49861 atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca
    49921 tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac
    49981 tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga
    50041 gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg
    50101 tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca
    50161 tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa
    50221 tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg
    50281 attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa
    50341 aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata
    50401 gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg actggcttgc
    50461 aatcgatgct tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc
    50521 aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg
    50581 gagaagataa ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca
    50641 ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc
    50701 ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa
    50761 caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag
    50821 gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg
    50881 ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga
    50941 ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa
    51001 aagcaatttt gccagtgact tatctatctc taggcggata catgcttgga ttgatactag
    51061 gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt
    51121 catatattat gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt
    51181 ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca
    51241 aatcgaatgt actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt
    51301 caattatgag gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg
    51361 gacatctatt gttcccagct tctttttgta tagacctatt ttatttattt tactatattg
    51421 gatatatatt cattgaaaat agtgtataac aactttataa tattgagtat accaattttt
    51481 ggaggcgttt taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag
    51541 gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag
    51601 ttatttttga gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt
    51661 tcaggaaatc ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat
    51721 atcagccaat tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac
    51781 aaagaacaag ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca
    51841 gtcaaatata cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt
    51901 gaggaactct tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc
    51961 attgaacaac ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg
    52021 atccaaaaat ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact
    52081 caagcagcca aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga
    52141 aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt
    52201 ttacggattg cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat
    52261 ttacgtccat tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct
    52321 aaaaacagta aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca
    52381 ctttctatcc ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa
    52441 atggacgatc gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa
    52501 ggtcagctct atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa
    52561 aaattcttgg aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct
    52621 ttgagaatgc aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac
    52681 aatcagcatg tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca
    52741 tttgaattag accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata
    52801 actgaaagcg caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat
    52861 gcgtggagtg ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt
    52921 tattttctca cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga
    52981 gatgaagagt ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca
    53041 gcaatcatta atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca
    53101 aatggaaaag tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga
    53161 attgccggtt attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg
    53221 tttttgccta ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta
    53281 atcgaacagc aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag
    53341 cagcagatta cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc
    53401 cagtgaacat tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt
    53461 ccggataaac ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca
    53521 tgaaaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    53581 ctcttggtat aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    53641 aatgtgaaaa gccattttca caaaattttt cttaatactc gaaactaaac agctttttca
    53701 caaccccctc agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca
    53761 gatggatgta cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata
    53821 tcatctttgc tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat
    53881 aaaggtgaaa gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg
    53941 agaaggttgc ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg
    54001 gcagtgggct gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa
    54061 agtgtacctt ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt
    54121 tttctttgtt tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt
    54181 gtttccgtta gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca
    54241 cctgcaagaa taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg
    54301 actggcagaa taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt
    54361 gtatagctca cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca
    54421 atgccgatga ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt
    54481 aaaaaaaatt aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta
    54541 tctgtaaaga gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg
    54601 ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct
    54661 tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag
    54721 gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga
    54781 agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg
    54841 ctcaaacact gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa
    54901 cgatccaaac aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg
    54961 aaatacaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag
    55021 caactttgaa agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa
    55081 gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa
    55141 agaaatcagg agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat
    55201 ctgtatcaaa atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt
    55261 attgataaag aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct
    55321 atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa
    55381 gaactttcct tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg
    55441 aaagaagtcc ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta
    55501 gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat
    55561 gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc
    55621 aaggaagaac tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa
    55681 caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct
    55741 gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg
    55801 caagagacca agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg
    55861 ttaggtcagt gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc
    55921 gttgcagaac gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg
    55981 caaggttggg tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta
    56041 tcagaagaag atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa
    56101 gtccctacta aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg
    56161 tacagtctgc caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt
    56221 gtcttcttcg ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca
    56281 atactagctc aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt
    56341 gagattctct cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag
    56401 tcattaccaa aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat
    56461 gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga
    56521 ttatttgtgg cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat
    56581 ggatttgcct ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg
    56641 ataaaaaata cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt
    56701 atcttgatcg ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg
    56761 tataatcttt acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg
    56821 gcattgggta tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg
    56881 ccgccagctg ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat
    56941 atgtttttga cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt
    57001 tttgggaagt tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa
    57061 tacgtcaaca tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg
    57121 atggattact taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca
    57181 gcgacgattt tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca
    57241 gcagcattga caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt
    57301 ccaggcacgc aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga
    57361 aatgacatgt cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt
    57421 acaggcttgt tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta
    57481 gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac
    57541 gcgattttag gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg
    57601 aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg
    57661 aaagagcggc gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac
    57721 gtacgcaagt tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa
    57781 aaaaataccg tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg
    57841 cgaagcagga atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg
    57901 atgaatctga acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa
    57961 aagtagcatt cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg
    58021 aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag
    58081 cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac
    58141 aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga
    58201 tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt
    58261 gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat
    58321 attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca
    58381 gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc
    58441 tgaaatcgtt tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa
    58501 agctgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt
    58561 agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat
    58621 ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga
    58681 cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata
    58741 tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc
    58801 tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg
    58861 aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa
    58921 ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat
    58981 tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc
    59041 acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga
    59101 aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat
    59161 cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt
    59221 ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg
    59281 ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc
    59341 aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta
    59401 tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa
    59461 acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc
    59521 aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg
    59581 ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga
    59641 tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg
    59701 aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac
    59761 aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt
    59821 tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac
    59881 caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa
    59941 caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa
    60001 tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg
    60061 acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa
    60121 aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag
    60181 tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc
    60241 agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc
    60301 gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac cctaatacag
    60361 gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag
    60421 ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt
    60481 atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga
    60541 gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc
    60601 ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag
    60661 gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca
    60721 cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa
    60781 aaagacattt tccttcgatc gattggatac aaagctattc tctttattca acagaagtag
    60841 ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc
    60901 gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc
    60961 tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc
    61021 agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt
    61081 tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt
    61141 taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata
    61201 tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc
    61261 aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa
    61321 gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc
    61381 gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat
    61441 aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta
    61501 cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc
    61561 gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat
    61621 attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca
    61681 ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt
    61741 tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta
    61801 ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa
    61861 gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt
    61921 atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact
    61981 gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg
    62041 accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga
    62101 agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga
    62161 atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat
    62221 aaaactcatc caatccctga cttgactggt tatattacag aaggacagat catcttgact
    62281 agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg
    62341 ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat
    62401 cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt
    62461 gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa
    62521 gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg
    62581 tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg
    62641 gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc
    62701 cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta
    62761 ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac
    62821 cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca
    62881 aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt
    62941 gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa
    63001 tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa
    63061 ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa
    63121 atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat
    63181 gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag
    63241 ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc
    63301 actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa
    63361 taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag
    63421 ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta
    63481 tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata
    63541 cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg
    63601 gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc
    63661 tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga
    63721 agctcatgct ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt
    63781 tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc
    63841 atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc
    63901 accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag
    63961 gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt
    64021 caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt
    64081 tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca
    64141 ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata
    64201 tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt
    64261 cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga
    64321 tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt
    64381 tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt
    64441 tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct
    64501 tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag
    64561 ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct
    64621 tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg
    64681 tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat
    64741 aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag
    64801 gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga
    64861 tttgattcct ttatttatca ataggttata attcatattt ttttattttt tcttttacga
    64921 tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg
    64981 catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa
    65041 gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt
    65101 aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggatc
    65161 tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt
    65221 gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt
    65281 ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa
    65341 taaattttgt cattccagct aagtagattg tttggttagg taataaattt tggaaaggag
    65401 gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc
    65461 atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa
    65521 accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac
    65581 attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct
    65641 ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta
    65701 ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg
    65761 aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg
    65821 cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat
    65881 ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag
    65941 aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg
    66001 actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt
    66061 tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag
    66121 ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt
    66181 gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc
    66241 gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt
    66301 gtataccata caattttacc attgattgta ttgattttag aagttaatct tttgtgagga
    66361 gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag
    66421 aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa
    66481 atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc
    66541 ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata
    66601 atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg
    66661 gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat
    66721 ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag
    66781 ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag
    66841 tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac
    66901 agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg
    66961 aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg
    67021 aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat
    67081 cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg
    67141 gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat
    67201 taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt
    67261 tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat
    67321 tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga
    67381 ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac
    67441 gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa
    67501 ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc
    67561 gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa
    67621 attttctact caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga
    67681 ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg
    67741 ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa
    67801 aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg
    67861 agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa
    67921 attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg
    67981 ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga
    68041 tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc
    68101 taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt
    68161 ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa
    68221 aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc
    68281 cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt
    68341 agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt
    68401 tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt
    68461 ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc
    68521 caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt
    68581 cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa
    68641 cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc aatcataaca
    68701 aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg
    68761 gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa
    68821 aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc
    68881 agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg
    68941 aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg
    69001 tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg
    69061 caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag
    69121 aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat
    69181 tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa
    69241 agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac atattttttg
    69301 attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg
    69361 acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat catggataag
    69421 tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg
    69481 agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc
    69541 gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca
    69601 tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt
    69661 tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa
    69721 gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg
    69781 ccgacacggc gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg
    69841 gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa
    69901 tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg
    69961 ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact
    70021 tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt
    70081 ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc gccattacgg
    70141 atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac
    70201 ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat
    70261 gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc
    70321 tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag
    70381 ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg
    70441 agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta
    70501 cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga
    70561 gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt
    70621 ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga
    70681 tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca
    70741 ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca
    70801 tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt
    70861 cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag
    70921 tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa
    70981 gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc
    71041 tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta
    71101 tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc
    71161 gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt
    71221 gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa
    71281 tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta
    71341 atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg
    71401 acatggatat agttatggac tggatcacga aaaacttttt ccataggcag catttgatat
    71461 ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat
    71521 ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa
    71581 atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac
    71641 aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg
    71701 atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg
    71761 cccacgtttt tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt
    71821 gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc
    71881 aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt
    71941 agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg
    72001 ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga
    72061 atttctgatc actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa
    72121 aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa
    72181 aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga
    72241 aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc
    72301 tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg
    72361 gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac
    72421 aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt
    72481 tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat
    72541 ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag
    72601 gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag
    72661 ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa
    72721 acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat
    72781 gttagtttga aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg
    72841 ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag
    72901 aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg
    72961 tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa
    73021 gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac
    73081 cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt
    73141 tatactgact tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg
    73201 cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag
    73261 gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat
    73321 gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat
    73381 gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct
    73441 gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat
    73501 atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa
    73561 gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga
    73621 cgatcagcct atcagaataa ggaggtgtga cacgattttt
//
LOCUS       DAJSVP010000004        70384 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSVP010000004 DAJSVP010000000
VERSION     DAJSVP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.004"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(185..1750)
                     /gene="guaA"
                     /locus_tag="ON449_000224"
     CDS             complement(185..1750)
                     /gene="guaA"
                     /locus_tag="ON449_000224"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3932846.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1809..2732)
                     /locus_tag="ON449_000225"
     CDS             complement(1809..2732)
                     /locus_tag="ON449_000225"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3932847.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2928..3533
                     /locus_tag="ON449_000226"
     CDS             2928..3533
                     /locus_tag="ON449_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3932848.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3841..4857
                     /locus_tag="ON449_000227"
     CDS             3841..4857
                     /locus_tag="ON449_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3932849.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4998..6299
                     /locus_tag="ON449_000228"
     CDS             4998..6299
                     /locus_tag="ON449_000228"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3932850.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6345..7007
                     /gene="deoC"
                     /locus_tag="ON449_000229"
     CDS             6345..7007
                     /gene="deoC"
                     /locus_tag="ON449_000229"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3932851.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7028..7426
                     /locus_tag="ON449_000230"
     CDS             7028..7426
                     /locus_tag="ON449_000230"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3932852.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7492..8574
                     /locus_tag="ON449_000231"
     CDS             7492..8574
                     /locus_tag="ON449_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3932853.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8786..10348
                     /locus_tag="ON449_000232"
     CDS             8786..10348
                     /locus_tag="ON449_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932854.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10341..11474
                     /locus_tag="ON449_000233"
     CDS             10341..11474
                     /locus_tag="ON449_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932855.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11474..12430
                     /locus_tag="ON449_000234"
     CDS             11474..12430
                     /locus_tag="ON449_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932856.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12529..13695
                     /gene="deoB"
                     /locus_tag="ON449_000235"
     CDS             12529..13695
                     /gene="deoB"
                     /locus_tag="ON449_000235"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3932857.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13719..14537
                     /locus_tag="ON449_000236"
     CDS             13719..14537
                     /locus_tag="ON449_000236"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3932858.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14554..15261
                     /gene="deoD"
                     /locus_tag="ON449_000237"
     CDS             14554..15261
                     /gene="deoD"
                     /locus_tag="ON449_000237"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3932859.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15559..16245)
                     /gene="gpmA"
                     /locus_tag="ON449_000238"
     CDS             complement(15559..16245)
                     /gene="gpmA"
                     /locus_tag="ON449_000238"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3932860.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16417..16998)
                     /locus_tag="ON449_000239"
     CDS             complement(16417..16998)
                     /locus_tag="ON449_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3932861.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17131..17814)
                     /gene="rpiA"
                     /locus_tag="ON449_000240"
     CDS             complement(17131..17814)
                     /gene="rpiA"
                     /locus_tag="ON449_000240"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3932862.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18133..18546
                     /gene="rpsL"
                     /locus_tag="ON449_000241"
     CDS             18133..18546
                     /gene="rpsL"
                     /locus_tag="ON449_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3932863.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18630..19100
                     /gene="rpsG"
                     /locus_tag="ON449_000242"
     CDS             18630..19100
                     /gene="rpsG"
                     /locus_tag="ON449_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3932864.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19194..21281
                     /gene="fusA"
                     /locus_tag="ON449_000243"
     CDS             19194..21281
                     /gene="fusA"
                     /locus_tag="ON449_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3932865.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21453..22640
                     /gene="tuf"
                     /locus_tag="ON449_000244"
     CDS             21453..22640
                     /gene="tuf"
                     /locus_tag="ON449_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3932866.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            23047..23355
                     /gene="rpsJ"
                     /locus_tag="ON449_000245"
     CDS             23047..23355
                     /gene="rpsJ"
                     /locus_tag="ON449_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3932867.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23386..24015
                     /gene="rplC"
                     /locus_tag="ON449_000246"
     CDS             23386..24015
                     /gene="rplC"
                     /locus_tag="ON449_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3932868.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            24043..24666
                     /gene="rplD"
                     /locus_tag="ON449_000247"
     CDS             24043..24666
                     /gene="rplD"
                     /locus_tag="ON449_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3932869.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24666..24956
                     /gene="rplW"
                     /locus_tag="ON449_000248"
     CDS             24666..24956
                     /gene="rplW"
                     /locus_tag="ON449_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3932870.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24995..25828
                     /gene="rplB"
                     /locus_tag="ON449_000249"
     CDS             24995..25828
                     /gene="rplB"
                     /locus_tag="ON449_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3932871.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25871..26149
                     /gene="rpsS"
                     /locus_tag="ON449_000250"
     CDS             25871..26149
                     /gene="rpsS"
                     /locus_tag="ON449_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3932872.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26172..26519
                     /gene="rplV"
                     /locus_tag="ON449_000251"
     CDS             26172..26519
                     /gene="rplV"
                     /locus_tag="ON449_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3932873.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26533..27189
                     /gene="rpsC"
                     /locus_tag="ON449_000252"
     CDS             26533..27189
                     /gene="rpsC"
                     /locus_tag="ON449_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3932874.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27192..27626
                     /gene="rplP"
                     /locus_tag="ON449_000253"
     CDS             27192..27626
                     /gene="rplP"
                     /locus_tag="ON449_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3932875.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27616..27804
                     /gene="rpmC"
                     /locus_tag="ON449_000254"
     CDS             27616..27804
                     /gene="rpmC"
                     /locus_tag="ON449_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3932876.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27829..28095
                     /gene="rpsQ"
                     /locus_tag="ON449_000255"
     CDS             27829..28095
                     /gene="rpsQ"
                     /locus_tag="ON449_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3932877.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28153..28521
                     /gene="rplN"
                     /locus_tag="ON449_000256"
     CDS             28153..28521
                     /gene="rplN"
                     /locus_tag="ON449_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3932878.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28558..28866
                     /gene="rplX"
                     /locus_tag="ON449_000257"
     CDS             28558..28866
                     /gene="rplX"
                     /locus_tag="ON449_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3932879.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28894..29433
                     /gene="rplE"
                     /locus_tag="ON449_000258"
     CDS             28894..29433
                     /gene="rplE"
                     /locus_tag="ON449_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3932880.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29452..29637
                     /locus_tag="ON449_000259"
     CDS             29452..29637
                     /locus_tag="ON449_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3932881.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29673..30071
                     /gene="rpsH"
                     /locus_tag="ON449_000260"
     CDS             29673..30071
                     /gene="rpsH"
                     /locus_tag="ON449_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3932882.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            30103..30639
                     /gene="rplF"
                     /locus_tag="ON449_000261"
     CDS             30103..30639
                     /gene="rplF"
                     /locus_tag="ON449_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3932883.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30800..31156
                     /gene="rplR"
                     /locus_tag="ON449_000262"
     CDS             30800..31156
                     /gene="rplR"
                     /locus_tag="ON449_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3932884.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31177..31677
                     /gene="rpsE"
                     /locus_tag="ON449_000263"
     CDS             31177..31677
                     /gene="rpsE"
                     /locus_tag="ON449_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3932885.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31692..31871
                     /gene="rpmD"
                     /locus_tag="ON449_000264"
     CDS             31692..31871
                     /gene="rpmD"
                     /locus_tag="ON449_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3932886.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31915..32355
                     /gene="rplO"
                     /locus_tag="ON449_000265"
     CDS             31915..32355
                     /gene="rplO"
                     /locus_tag="ON449_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3932887.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32355..33650
                     /gene="secY"
                     /locus_tag="ON449_000266"
     CDS             32355..33650
                     /gene="secY"
                     /locus_tag="ON449_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3932888.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33712..34359
                     /locus_tag="ON449_000267"
     CDS             33712..34359
                     /locus_tag="ON449_000267"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3932889.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34550..34768
                     /gene="infA"
                     /locus_tag="ON449_000268"
     CDS             34550..34768
                     /gene="infA"
                     /locus_tag="ON449_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3932890.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34801..34917
                     /gene="rpmJ"
                     /locus_tag="ON449_000269"
     CDS             34801..34917
                     /gene="rpmJ"
                     /locus_tag="ON449_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3932891.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34935..35300
                     /gene="rpsM"
                     /locus_tag="ON449_000270"
     CDS             34935..35300
                     /gene="rpsM"
                     /locus_tag="ON449_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3932892.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35328..35717
                     /gene="rpsK"
                     /locus_tag="ON449_000271"
     CDS             35328..35717
                     /gene="rpsK"
                     /locus_tag="ON449_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3932893.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35797..36735
                     /locus_tag="ON449_000272"
     CDS             35797..36735
                     /locus_tag="ON449_000272"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3932894.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36763..37143
                     /gene="rplQ"
                     /locus_tag="ON449_000273"
     CDS             36763..37143
                     /gene="rplQ"
                     /locus_tag="ON449_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3932895.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37415..38254
                     /locus_tag="ON449_000274"
     CDS             37415..38254
                     /locus_tag="ON449_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3932896.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38230..39099
                     /locus_tag="ON449_000275"
     CDS             38230..39099
                     /locus_tag="ON449_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3932897.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            39089..39886
                     /locus_tag="ON449_000276"
     CDS             39089..39886
                     /locus_tag="ON449_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3932898.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            40089..40835
                     /gene="truA"
                     /locus_tag="ON449_000277"
     CDS             40089..40835
                     /gene="truA"
                     /locus_tag="ON449_000277"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3932899.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41290..43959)
                     /locus_tag="ON449_000278"
     CDS             complement(41290..43959)
                     /locus_tag="ON449_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3932900.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(44197..44385)
                     /locus_tag="ON449_000279"
     CDS             complement(44197..44385)
                     /locus_tag="ON449_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3932901.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44655..45017
                     /locus_tag="ON449_000280"
     CDS             44655..45017
                     /locus_tag="ON449_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3932902.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            45069..46751
                     /locus_tag="ON449_000281"
     CDS             45069..46751
                     /locus_tag="ON449_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3932903.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46868..48904
                     /gene="recG"
                     /locus_tag="ON449_000282"
     CDS             46868..48904
                     /gene="recG"
                     /locus_tag="ON449_000282"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3932904.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            48953..49954
                     /gene="plsX"
                     /locus_tag="ON449_000283"
     CDS             48953..49954
                     /gene="plsX"
                     /locus_tag="ON449_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3932905.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            50076..50318
                     /gene="acpP"
                     /locus_tag="ON449_000284"
     CDS             50076..50318
                     /gene="acpP"
                     /locus_tag="ON449_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3932906.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            50586..51590
                     /locus_tag="ON449_000285"
     CDS             50586..51590
                     /locus_tag="ON449_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932907.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            51591..52535
                     /locus_tag="ON449_000286"
     CDS             51591..52535
                     /locus_tag="ON449_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932908.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            52535..53497
                     /locus_tag="ON449_000287"
     CDS             52535..53497
                     /locus_tag="ON449_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932909.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            53524..54438
                     /locus_tag="ON449_000288"
     CDS             53524..54438
                     /locus_tag="ON449_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932910.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            54464..56245
                     /locus_tag="ON449_000289"
     CDS             54464..56245
                     /locus_tag="ON449_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3932911.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            56593..57279
                     /locus_tag="ON449_000290"
     CDS             56593..57279
                     /locus_tag="ON449_000290"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3932912.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            57299..60880
                     /gene="smc"
                     /locus_tag="ON449_000291"
     CDS             57299..60880
                     /gene="smc"
                     /locus_tag="ON449_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3932913.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            60889..61698
                     /locus_tag="ON449_000292"
     CDS             60889..61698
                     /locus_tag="ON449_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3932914.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            61709..62707
                     /gene="ftsY"
                     /locus_tag="ON449_000293"
     CDS             61709..62707
                     /gene="ftsY"
                     /locus_tag="ON449_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3932915.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            62894..65161
                     /gene="gshAB"
                     /locus_tag="ON449_000294"
     CDS             62894..65161
                     /gene="gshAB"
                     /locus_tag="ON449_000294"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3932916.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(65250..65702)
                     /locus_tag="ON449_000295"
     CDS             complement(65250..65702)
                     /locus_tag="ON449_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3932917.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            65852..66802
                     /locus_tag="ON449_000296"
     CDS             65852..66802
                     /locus_tag="ON449_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3932918.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            66795..67754
                     /locus_tag="ON449_000297"
     CDS             66795..67754
                     /locus_tag="ON449_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3932919.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            67751..68506
                     /locus_tag="ON449_000298"
     CDS             67751..68506
                     /locus_tag="ON449_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3932920.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            68529..69482
                     /locus_tag="ON449_000299"
     CDS             68529..69482
                     /locus_tag="ON449_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3932921.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(70126..70314)
                     /locus_tag="ON449_000300"
     CDS             complement(70126..70314)
                     /locus_tag="ON449_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932922.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
BASE COUNT        22835 a        12809 c        15272 g        19468 t
ORIGIN      
        1 aagctaattc aataggaaaa taaaggtagt gaataaaagc aaagcccttg ttagtagtta
       61 atctaacaag ggctttggta gggaaaatgg taggaaacca tcttattttt tacgaaaatc
      121 aggaacttta gtaattgaat aatgttgatt tataggtatt cctcatccat attatgttgg
      181 ctctttattc ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt
      241 tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga
      301 tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt
      361 agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga
      421 aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat
      481 cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa
      541 gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc
      601 ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca
      661 tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac
      721 cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac
      781 cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac
      841 gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcattga
      901 ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc
      961 ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt
     1021 gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt
     1081 tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc
     1141 agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt
     1201 cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga
     1261 tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag
     1321 ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga
     1381 cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct
     1441 ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag
     1501 gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt
     1561 ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa
     1621 tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt
     1681 ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa
     1741 cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt
     1801 ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg
     1861 agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc
     1921 acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa
     1981 cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc
     2041 aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag
     2101 tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc
     2161 aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt
     2221 gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc
     2281 agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt
     2341 aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg
     2401 aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca
     2461 cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct
     2521 ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt
     2581 acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct
     2641 agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt
     2701 gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg
     2761 gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt
     2821 ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca
     2881 taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt
     2941 actattctga acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag
     3001 gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg
     3061 gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct
     3121 tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt
     3181 ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc
     3241 gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag
     3301 aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg
     3361 aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc
     3421 agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag
     3481 aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg
     3541 aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt
     3601 ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt
     3661 aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg
     3721 ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta
     3781 ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg
     3841 atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca
     3901 cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg
     3961 atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat
     4021 tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc
     4081 gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt
     4141 catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga
     4201 atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat
     4261 caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt
     4321 atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat
     4381 acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac
     4441 ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat
     4501 ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat
     4561 atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat
     4621 gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg
     4681 tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt
     4741 tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa
     4801 gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc
     4861 ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag
     4921 taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag
     4981 acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg
     5041 agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag
     5101 attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa
     5161 tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg
     5221 aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt
     5281 tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag
     5341 gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt
     5401 cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag
     5461 gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg
     5521 attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg
     5581 caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa
     5641 gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg
     5701 ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg
     5761 aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct
     5821 taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt
     5881 tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa
     5941 atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca
     6001 caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag
     6061 gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg
     6121 ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat
     6181 tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata
     6241 ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa
     6301 ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa
     6361 tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg
     6421 aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag
     6481 cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag
     6541 gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag
     6601 atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc
     6661 aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta
     6721 ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg
     6781 caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg
     6841 atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta
     6901 tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa
     6961 gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga
     7021 gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa
     7081 agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa
     7141 gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg
     7201 cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc
     7261 ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt
     7321 ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac
     7381 cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca
     7441 gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa
     7501 gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc
     7561 ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca
     7621 gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa
     7681 ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat
     7741 ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga
     7801 ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca
     7861 aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt
     7921 gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat
     7981 acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt
     8041 tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc
     8101 aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct
     8161 caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac
     8221 ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc
     8281 ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac
     8341 ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa
     8401 tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt
     8461 gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa
     8521 gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat
     8581 ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg
     8641 ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg
     8701 tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata
     8761 gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa
     8821 tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc
     8881 aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt
     8941 gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa
     9001 gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat
     9061 gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc
     9121 agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg
     9181 attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt
     9241 agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc
     9301 tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga
     9361 aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg
     9421 ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc
     9481 acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga
     9541 agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg
     9601 attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc
     9661 tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc
     9721 agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat
     9781 cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga
     9841 tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa
     9901 cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga
     9961 tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa
    10021 agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac
    10081 tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga
    10141 caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga
    10201 tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc
    10261 tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt
    10321 agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc
    10381 ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt
    10441 ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga
    10501 taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag
    10561 ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg
    10621 tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat
    10681 gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag
    10741 cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg
    10801 taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt
    10861 ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg
    10921 gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc
    10981 tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt
    11041 ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg
    11101 ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc
    11161 aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat
    11221 cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg
    11281 gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta
    11341 tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga
    11401 aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag
    11461 ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt
    11521 ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat
    11581 cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa
    11641 cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg
    11701 cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga
    11761 ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat
    11821 caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc
    11881 tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt
    11941 acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa
    12001 attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat
    12061 caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg
    12121 cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca
    12181 agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg
    12241 tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt
    12301 tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga caatcattgt
    12361 gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa
    12421 atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc
    12481 cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc
    12541 gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc
    12601 ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca
    12661 catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa
    12721 gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca
    12781 ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga
    12841 ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac
    12901 aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc
    12961 gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc
    13021 attcctttag aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat
    13081 ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca
    13141 cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct
    13201 ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga
    13261 caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg
    13321 aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca
    13381 ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta
    13441 cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat
    13501 cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg
    13561 gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt
    13621 tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg
    13681 aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt
    13741 tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga
    13801 ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt
    13861 atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt
    13921 atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag
    13981 gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt
    14041 cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga
    14101 tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag
    14161 atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa
    14221 cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct
    14281 attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg
    14341 atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag
    14401 tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg
    14461 aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa
    14521 cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa
    14581 ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg
    14641 gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc
    14701 ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc
    14761 accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt
    14821 acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca
    14881 gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt
    14941 gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat
    15001 gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa
    15061 ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc
    15121 gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt
    15181 gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg
    15241 gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga
    15301 acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg
    15361 taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca
    15421 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga
    15481 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
    15541 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
    15601 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
    15661 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
    15721 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
    15781 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
    15841 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
    15901 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
    15961 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
    16021 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
    16081 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
    16141 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
    16201 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
    16261 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
    16321 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
    16381 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
    16441 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
    16501 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
    16561 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
    16621 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
    16681 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
    16741 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
    16801 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
    16861 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
    16921 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
    16981 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
    17041 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
    17101 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
    17161 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
    17221 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
    17281 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
    17341 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
    17401 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
    17461 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
    17521 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
    17581 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
    17641 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
    17701 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
    17761 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
    17821 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
    17881 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
    17941 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
    18001 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
    18061 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
    18121 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
    18181 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
    18241 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
    18301 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
    18361 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
    18421 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
    18481 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
    18541 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
    18601 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
    18661 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
    18721 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
    18781 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
    18841 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
    18901 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
    18961 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
    19021 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
    19081 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
    19141 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
    19201 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
    19261 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
    19321 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
    19381 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
    19441 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
    19501 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
    19561 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
    19621 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
    19681 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
    19741 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
    19801 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
    19861 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
    19921 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
    19981 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
    20041 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
    20101 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
    20161 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
    20221 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
    20281 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
    20341 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
    20401 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
    20461 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
    20521 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
    20581 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
    20641 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
    20701 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
    20761 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
    20821 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
    20881 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
    20941 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
    21001 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
    21061 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
    21121 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
    21181 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
    21241 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
    21301 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
    21361 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
    21421 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
    21481 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
    21541 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
    21601 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
    21661 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
    21721 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
    21781 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
    21841 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
    21901 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
    21961 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
    22021 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
    22081 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
    22141 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
    22201 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
    22261 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
    22321 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
    22381 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
    22441 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
    22501 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
    22561 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
    22621 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
    22681 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
    22741 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
    22801 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
    22861 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
    22921 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
    22981 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
    23041 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
    23101 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
    23161 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
    23221 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
    23281 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
    23341 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
    23401 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
    23461 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
    23521 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
    23581 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
    23641 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
    23701 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
    23761 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
    23821 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
    23881 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
    23941 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
    24001 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
    24061 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
    24121 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
    24181 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
    24241 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
    24301 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
    24361 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
    24421 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
    24481 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
    24541 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
    24601 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
    24661 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
    24721 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
    24781 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
    24841 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
    24901 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
    24961 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
    25021 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
    25081 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
    25141 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
    25201 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
    25261 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
    25321 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
    25381 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
    25441 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    25501 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    25561 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    25621 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    25681 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    25741 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    25801 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    25861 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    25921 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    25981 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    26041 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    26101 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    26161 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    26221 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    26281 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    26341 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    26401 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    26461 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    26521 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    26581 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    26641 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    26701 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    26761 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    26821 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    26881 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    26941 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    27001 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    27061 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    27121 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    27181 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    27241 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    27301 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    27361 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    27421 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    27481 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    27541 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    27601 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    27661 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    27721 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    27781 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    27841 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    27901 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    27961 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    28021 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    28081 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    28141 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    28201 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    28261 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    28321 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    28381 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    28441 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    28501 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    28561 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    28621 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    28681 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    28741 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    28801 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    28861 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    28921 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    28981 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    29041 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    29101 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    29161 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    29221 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    29281 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    29341 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    29401 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    29461 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    29521 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    29581 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    29641 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    29701 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    29761 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    29821 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    29881 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    29941 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    30001 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    30061 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    30121 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    30181 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    30241 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    30301 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    30361 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    30421 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    30481 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    30541 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    30601 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    30661 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    30721 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    30781 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    30841 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    30901 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    30961 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    31021 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    31081 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    31141 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    31201 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    31261 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    31321 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    31381 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    31441 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    31501 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    31561 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    31621 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    31681 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    31741 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    31801 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    31861 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    31921 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    31981 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    32041 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    32101 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    32161 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    32221 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    32281 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    32341 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    32401 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    32461 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    32521 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    32581 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    32641 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    32701 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    32761 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    32821 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    32881 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    32941 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    33001 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    33061 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    33121 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    33181 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    33241 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    33301 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    33361 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    33421 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    33481 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    33541 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    33601 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    33661 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    33721 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    33781 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    33841 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    33901 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    33961 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    34021 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    34081 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    34141 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    34201 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    34261 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    34321 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    34381 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    34441 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    34501 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    34561 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    34621 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    34681 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    34741 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    34801 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    34861 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    34921 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    34981 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    35041 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    35101 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    35161 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    35221 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    35281 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    35341 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    35401 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    35461 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    35521 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    35581 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    35641 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    35701 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    35761 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    35821 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    35881 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    35941 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    36001 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    36061 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    36121 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    36181 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    36241 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    36301 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    36361 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    36421 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    36481 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    36541 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    36601 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    36661 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    36721 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    36781 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    36841 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    36901 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    36961 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    37021 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    37081 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    37141 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    37201 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    37261 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    37321 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    37381 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    37441 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    37501 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    37561 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    37621 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    37681 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    37741 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    37801 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    37861 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    37921 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    37981 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    38041 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    38101 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    38161 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    38221 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    38281 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    38341 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    38401 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    38461 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    38521 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    38581 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    38641 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    38701 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    38761 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    38821 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    38881 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    38941 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    39001 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    39061 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    39121 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    39181 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    39241 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    39301 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    39361 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    39421 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    39481 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    39541 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    39601 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    39661 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    39721 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    39781 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    39841 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    39901 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    39961 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    40021 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    40081 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    40141 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    40201 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    40261 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    40321 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    40381 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    40441 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    40501 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    40561 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    40621 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    40681 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    40741 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    40801 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    40861 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    40921 attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt
    40981 tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga
    41041 gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat
    41101 ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg
    41161 tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa
    41221 ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc
    41281 atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc
    41341 tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa
    41401 caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact
    41461 gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt
    41521 gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt
    41581 tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc
    41641 ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc
    41701 tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt
    41761 aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt
    41821 gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg
    41881 cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc
    41941 ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt
    42001 ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg
    42061 ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac
    42121 tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac
    42181 attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa
    42241 cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt
    42301 attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag
    42361 gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata
    42421 gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga
    42481 atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag tatataaact
    42541 gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg tattgaccag
    42601 tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt
    42661 atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa
    42721 ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa
    42781 gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt
    42841 ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa
    42901 taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa
    42961 acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt
    43021 ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg
    43081 aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag
    43141 ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg
    43201 aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt
    43261 gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat
    43321 aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa
    43381 gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc
    43441 caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc
    43501 agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat
    43561 cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt
    43621 cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa
    43681 gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca
    43741 tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga
    43801 aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat
    43861 acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg
    43921 tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc
    43981 tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt
    44041 ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata
    44101 aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa
    44161 accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga
    44221 tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac
    44281 tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt
    44341 tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt
    44401 tgccttgatt tggagcaata gaataattca gctcatacca gactaacata tcatatagaa
    44461 tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa
    44521 ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt
    44581 tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt
    44641 aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca
    44701 atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa
    44761 tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact
    44821 caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta
    44881 tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca
    44941 atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc
    45001 gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga
    45061 ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag
    45121 gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc
    45181 cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag
    45241 tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga
    45301 tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc
    45361 aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag
    45421 ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta
    45481 gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa
    45541 tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag
    45601 tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg
    45661 gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag
    45721 cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga
    45781 cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta
    45841 cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt
    45901 ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag
    45961 gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca
    46021 tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa
    46081 aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag
    46141 agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa
    46201 gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc
    46261 caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag
    46321 tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg
    46381 atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga
    46441 gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag
    46501 gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa
    46561 cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg
    46621 caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac
    46681 atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt
    46741 ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc
    46801 ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga
    46861 aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag
    46921 agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc
    46981 ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt
    47041 tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag
    47101 ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca
    47161 accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga
    47221 tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt
    47281 ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa
    47341 aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga
    47401 gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca
    47461 cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat
    47521 gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta
    47581 tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca
    47641 aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact
    47701 tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac
    47761 cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca
    47821 tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg
    47881 gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa
    47941 cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct
    48001 agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa
    48061 aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat
    48121 tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc
    48181 gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa
    48241 agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc
    48301 attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa
    48361 atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga
    48421 agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt
    48481 aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca
    48541 gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt
    48601 tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa
    48661 tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt
    48721 ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt
    48781 ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc
    48841 tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga
    48901 ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat
    48961 tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt
    49021 agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa
    49081 gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag
    49141 tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc
    49201 acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct
    49261 actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat
    49321 gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc
    49381 taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc
    49441 acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc
    49501 aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt
    49561 gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt
    49621 cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat
    49681 gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat
    49741 gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg
    49801 tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga
    49861 tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca
    49921 attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt
    49981 ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt
    50041 aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca
    50101 aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg acggatgaat
    50161 tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc gttttagagc
    50221 ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg
    50281 gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg
    50341 ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt
    50401 catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct
    50461 atataattat taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt
    50521 attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta
    50581 ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca
    50641 aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt
    50701 tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat
    50761 tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa
    50821 tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc
    50881 aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac
    50941 tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag
    51001 aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag
    51061 gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca
    51121 ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac
    51181 tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag
    51241 tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag
    51301 caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact
    51361 ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt
    51421 ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag
    51481 caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt
    51541 gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat
    51601 taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag
    51661 taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat
    51721 atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat
    51781 tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca
    51841 accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca
    51901 gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac
    51961 ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc
    52021 cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag
    52081 gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg
    52141 ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat
    52201 acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca
    52261 atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata
    52321 atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag
    52381 aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc
    52441 aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac
    52501 acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt
    52561 tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc
    52621 gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga
    52681 acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat
    52741 cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga
    52801 cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac
    52861 ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga
    52921 taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt
    52981 gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac
    53041 actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt
    53101 accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga
    53161 agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac
    53221 gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat
    53281 ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta
    53341 tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac
    53401 atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat
    53461 catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga
    53521 tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga
    53581 atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg
    53641 gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat
    53701 gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc
    53761 attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga
    53821 aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta
    53881 gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat
    53941 tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga
    54001 tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga
    54061 ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca
    54121 atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta
    54181 atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact
    54241 ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca
    54301 agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt
    54361 ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg
    54421 cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt
    54481 tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga
    54541 tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat
    54601 tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac
    54661 ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc
    54721 ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga
    54781 cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa
    54841 agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta
    54901 cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat
    54961 cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat
    55021 agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg
    55081 tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga
    55141 tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag
    55201 taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac
    55261 gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt
    55321 gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa
    55381 agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt
    55441 caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat
    55501 ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga
    55561 acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc
    55621 ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt
    55681 agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa
    55741 taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga
    55801 ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct
    55861 gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta
    55921 ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc
    55981 tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag
    56041 ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata
    56101 tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa
    56161 cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc
    56221 tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt
    56281 ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc
    56341 atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg
    56401 caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt
    56461 tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac
    56521 catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa
    56581 ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt
    56641 tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat
    56701 cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa
    56761 ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca
    56821 aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac
    56881 tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca
    56941 gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc
    57001 gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt
    57061 tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta
    57121 tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc
    57181 gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca
    57241 gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt
    57301 gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga
    57361 ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac
    57421 agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat
    57481 gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac
    57541 agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac
    57601 ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt
    57661 aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat
    57721 ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt
    57781 tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct
    57841 ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca
    57901 gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac
    57961 actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg
    58021 ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc
    58081 gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg
    58141 tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga
    58201 aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata
    58261 tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag
    58321 cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat
    58381 aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt
    58441 aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta
    58501 tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc
    58561 ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca
    58621 ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa
    58681 cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa
    58741 tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt
    58801 tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt
    58861 cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc
    58921 tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat
    58981 cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa
    59041 accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg
    59101 gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct
    59161 aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg
    59221 ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac
    59281 aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa
    59341 acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt
    59401 tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg
    59461 aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa
    59521 tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca
    59581 gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct
    59641 tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca
    59701 gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc
    59761 tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa
    59821 agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga
    59881 cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc
    59941 tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca
    60001 aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg
    60061 actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa
    60121 tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta
    60181 tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat
    60241 tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag
    60301 acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga
    60361 aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat
    60421 ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt
    60481 actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg
    60541 caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc
    60601 gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga
    60661 agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga
    60721 tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact
    60781 gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga
    60841 tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta
    60901 gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat
    60961 aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc
    61021 cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac
    61081 agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag
    61141 aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg
    61201 ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa
    61261 tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt
    61321 acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct
    61381 aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt
    61441 atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca
    61501 aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta
    61561 ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa
    61621 gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa
    61681 aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt
    61741 tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga
    61801 aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt
    61861 cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga
    61921 aacagcgata cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa
    61981 atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg
    62041 aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt
    62101 cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa
    62161 cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca
    62221 gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga
    62281 tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt
    62341 actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa
    62401 aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt
    62461 agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga
    62521 tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc
    62581 tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga
    62641 cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga
    62701 agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat
    62761 gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg
    62821 ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat
    62881 tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt
    62941 tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa
    63001 tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca
    63061 aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga
    63121 attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga
    63181 gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat
    63241 tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg
    63301 gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct
    63361 aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact
    63421 ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg
    63481 tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt
    63541 tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta
    63601 tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc
    63661 ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt
    63721 agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag
    63781 attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg
    63841 gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa
    63901 agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct
    63961 tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga
    64021 ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga
    64081 agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga
    64141 aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat
    64201 atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca
    64261 atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag
    64321 taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt
    64381 aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt
    64441 aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta
    64501 cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt
    64561 agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga
    64621 atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa
    64681 tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc
    64741 tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca
    64801 attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt
    64861 ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga
    64921 attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat
    64981 tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc
    65041 atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc
    65101 aggaaaagac agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta
    65161 aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt
    65221 gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc
    65281 ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata
    65341 tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga
    65401 tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg
    65461 atatattggt tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga
    65521 tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca
    65581 gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg
    65641 ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc
    65701 ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa
    65761 tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg
    65821 aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt
    65881 agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact
    65941 gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat
    66001 ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac
    66061 ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat
    66121 tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt
    66181 ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa
    66241 aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc
    66301 tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa
    66361 ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct
    66421 cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt
    66481 aaatctggga atagattacc agaagatgca gtttttaggc attggcttag tatcattggc
    66541 tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa
    66601 tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc
    66661 aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga
    66721 aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat
    66781 aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg
    66841 ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat
    66901 gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg
    66961 ccacaatcag ctttcagaca attacgcata accagttttt gactccttca gtattgggac
    67021 ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga
    67081 tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga
    67141 gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga
    67201 tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca
    67261 tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc
    67321 agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc
    67381 gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga
    67441 tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat
    67501 caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc
    67561 gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc
    67621 tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga
    67681 gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga
    67741 agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt
    67801 taaagatgta cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg
    67861 tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga
    67921 aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt
    67981 atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga
    68041 atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca
    68101 ggaatcactg gaacaccttg ggttagaaga aatagcggat caatcgattg acactctttc
    68161 aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat
    68221 tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat
    68281 tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa
    68341 ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca
    68401 aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat
    68461 acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga
    68521 gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag
    68581 catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg
    68641 aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag
    68701 aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag
    68761 ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag
    68821 tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag
    68881 atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc
    68941 cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca
    69001 ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat
    69061 ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca
    69121 cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc
    69181 atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa
    69241 gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa
    69301 caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac
    69361 aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa
    69421 gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat
    69481 agcataaata acagaggatt aaactctcat actatgtaac aataatgttt cttacattct
    69541 taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga
    69601 tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc
    69661 tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt
    69721 cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa
    69781 cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct
    69841 gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt
    69901 tcttattact caaaacaaat cgcagttgtc acaatctctt ctaaacgatg ttccatctgc
    69961 tgcttctcgg caataagccc actagactag aaaatatgag aagctatttt ctgtccagcg
    70021 ttcttattac tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat gttcaatcag
    70081 ctgaccttcg gtattagctc aaagcactga tttcacaacc tctatttagg catgtgtctc
    70141 agcaagcggt tgatagttga tgatctgcat ttttcgataa aaatcggcgg gaagcttact
    70201 ccaccgtttt agtaattgat agaaataact ctcttctgct ttgattttat actcgcttaa
    70261 tatcaatcca aaactattcg ttttttttga agctaatttt ttataaatat ccaatgcttt
    70321 ggttttatct tcattgacaa agagtgtttt tacttcaata taagaaccct cgatattttg
    70381 tgtt
//
LOCUS       DAJSVP010000005        68304 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSVP010000005 DAJSVP010000000
VERSION     DAJSVP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.005"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            18..899
                     /locus_tag="ON449_000301"
     CDS             18..899
                     /locus_tag="ON449_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932923.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTILGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ON449_000302"
     CDS             912..2522
                     /locus_tag="ON449_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3932924.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ON449_000303"
     CDS             complement(2599..3258)
                     /locus_tag="ON449_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3932925.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ON449_000304"
     CDS             complement(3541..4206)
                     /locus_tag="ON449_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3932926.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ON449_000305"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ON449_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3932927.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ON449_000306"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ON449_000306"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3932928.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6417)
                     /gene="dhaM"
                     /locus_tag="ON449_000307"
     CDS             complement(5971..6417)
                     /gene="dhaM"
                     /locus_tag="ON449_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3932929.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ON449_000308"
     CDS             6520..6840
                     /locus_tag="ON449_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3932930.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7821)
                     /locus_tag="ON449_000309"
     CDS             complement(6952..7821)
                     /locus_tag="ON449_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3932931.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7917..9221)
                     /locus_tag="ON449_000310"
     CDS             complement(7917..9221)
                     /locus_tag="ON449_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3932932.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9218..9727)
                     /locus_tag="ON449_000311"
     CDS             complement(9218..9727)
                     /locus_tag="ON449_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932933.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9902..12037)
                     /locus_tag="ON449_000312"
     CDS             complement(9902..12037)
                     /locus_tag="ON449_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3932934.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12120..13775)
                     /locus_tag="ON449_000313"
     CDS             complement(12120..13775)
                     /locus_tag="ON449_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3932935.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13777..14091)
                     /locus_tag="ON449_000314"
     CDS             complement(13777..14091)
                     /locus_tag="ON449_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3932936.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14261..14521
                     /locus_tag="ON449_000315"
     CDS             14261..14521
                     /locus_tag="ON449_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3932937.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14535..14849
                     /locus_tag="ON449_000316"
     CDS             14535..14849
                     /locus_tag="ON449_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3932938.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15579..16133)
                     /locus_tag="ON449_000317"
     CDS             complement(15579..16133)
                     /locus_tag="ON449_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3932939.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16259..18187)
                     /locus_tag="ON449_000318"
     CDS             complement(16259..18187)
                     /locus_tag="ON449_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3932940.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18493..19209)
                     /locus_tag="ON449_000319"
     CDS             complement(18493..19209)
                     /locus_tag="ON449_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3932941.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19210..21033)
                     /gene="glpO"
                     /locus_tag="ON449_000320"
     CDS             complement(19210..21033)
                     /gene="glpO"
                     /locus_tag="ON449_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3932942.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21047..22543)
                     /gene="glpK"
                     /locus_tag="ON449_000321"
     CDS             complement(21047..22543)
                     /gene="glpK"
                     /locus_tag="ON449_000321"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3932943.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22679..24139)
                     /locus_tag="ON449_000322"
     CDS             complement(22679..24139)
                     /locus_tag="ON449_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3932944.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24129..25424)
                     /locus_tag="ON449_000323"
     CDS             complement(24129..25424)
                     /locus_tag="ON449_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3932945.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25756..26433)
                     /locus_tag="ON449_000324"
     CDS             complement(25756..26433)
                     /locus_tag="ON449_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3932946.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26463..27554)
                     /locus_tag="ON449_000325"
     CDS             complement(26463..27554)
                     /locus_tag="ON449_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3932947.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27655..28089)
                     /locus_tag="ON449_000326"
     CDS             complement(27655..28089)
                     /locus_tag="ON449_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3932948.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28673..30130)
                     /gene="ascB"
                     /locus_tag="ON449_000327"
     CDS             complement(28673..30130)
                     /gene="ascB"
                     /locus_tag="ON449_000327"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3932949.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30127..32022)
                     /locus_tag="ON449_000328"
     CDS             complement(30127..32022)
                     /locus_tag="ON449_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3932950.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32080..33855)
                     /locus_tag="ON449_000329"
     CDS             complement(32080..33855)
                     /locus_tag="ON449_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3932951.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33905..35401)
                     /locus_tag="ON449_000330"
     CDS             complement(33905..35401)
                     /locus_tag="ON449_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3932952.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35595..37217)
                     /locus_tag="ON449_000331"
     CDS             complement(35595..37217)
                     /locus_tag="ON449_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3932953.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37666..38979)
                     /locus_tag="ON449_000332"
     CDS             complement(37666..38979)
                     /locus_tag="ON449_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3932954.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39224..39448)
                     /locus_tag="ON449_000333"
     CDS             complement(39224..39448)
                     /locus_tag="ON449_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932955.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39468..39890)
                     /locus_tag="ON449_000334"
     CDS             complement(39468..39890)
                     /locus_tag="ON449_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3932956.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40033..40743)
                     /locus_tag="ON449_000335"
     CDS             complement(40033..40743)
                     /locus_tag="ON449_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932957.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40761..41258)
                     /locus_tag="ON449_000336"
     CDS             complement(40761..41258)
                     /locus_tag="ON449_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3932958.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41295..41747)
                     /locus_tag="ON449_000337"
     CDS             complement(41295..41747)
                     /locus_tag="ON449_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3932959.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41882..42487)
                     /locus_tag="ON449_000338"
     CDS             complement(41882..42487)
                     /locus_tag="ON449_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3932960.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42937..43557)
                     /locus_tag="ON449_000339"
     CDS             complement(42937..43557)
                     /locus_tag="ON449_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3932961.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43559..44428)
                     /locus_tag="ON449_000340"
     CDS             complement(43559..44428)
                     /locus_tag="ON449_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3932962.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44451..44858)
                     /locus_tag="ON449_000341"
     CDS             complement(44451..44858)
                     /locus_tag="ON449_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3932963.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44877..46166)
                     /locus_tag="ON449_000342"
     CDS             complement(44877..46166)
                     /locus_tag="ON449_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3932964.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46193..46495)
                     /locus_tag="ON449_000343"
     CDS             complement(46193..46495)
                     /locus_tag="ON449_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3932965.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46520..48613)
                     /locus_tag="ON449_000344"
     CDS             complement(46520..48613)
                     /locus_tag="ON449_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3932966.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49102..49866)
                     /locus_tag="ON449_000345"
     CDS             complement(49102..49866)
                     /locus_tag="ON449_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3932967.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49977..50846)
                     /gene="mdcB"
                     /locus_tag="ON449_000346"
     CDS             complement(49977..50846)
                     /gene="mdcB"
                     /locus_tag="ON449_000346"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3932968.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50871..51947)
                     /locus_tag="ON449_000347"
     CDS             complement(50871..51947)
                     /locus_tag="ON449_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3932969.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51971..52564)
                     /locus_tag="ON449_000348"
     CDS             complement(51971..52564)
                     /locus_tag="ON449_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3932970.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52568..54244)
                     /gene="mdcD"
                     /locus_tag="ON449_000349"
     CDS             complement(52568..54244)
                     /gene="mdcD"
                     /locus_tag="ON449_000349"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3932971.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54234..54533)
                     /locus_tag="ON449_000350"
     CDS             complement(54234..54533)
                     /locus_tag="ON449_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3932972.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54635..56281)
                     /gene="mdcA"
                     /locus_tag="ON449_000351"
     CDS             complement(54635..56281)
                     /gene="mdcA"
                     /locus_tag="ON449_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR3932973.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56288..57199)
                     /locus_tag="ON449_000352"
     CDS             complement(56288..57199)
                     /locus_tag="ON449_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3932974.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57319..58176
                     /locus_tag="ON449_000353"
     CDS             57319..58176
                     /locus_tag="ON449_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3932975.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58363..58725)
                     /locus_tag="ON449_000354"
     CDS             complement(58363..58725)
                     /locus_tag="ON449_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3932976.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59071..60510
                     /locus_tag="ON449_000355"
     CDS             59071..60510
                     /locus_tag="ON449_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3932977.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60517..63069)
                     /locus_tag="ON449_000356"
     CDS             complement(60517..63069)
                     /locus_tag="ON449_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR3932978.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63183..64514)
                     /locus_tag="ON449_000357"
     CDS             complement(63183..64514)
                     /locus_tag="ON449_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3932979.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64480..65958)
                     /locus_tag="ON449_000358"
     CDS             complement(64480..65958)
                     /locus_tag="ON449_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3932980.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66263..68098)
                     /gene="tdc"
                     /locus_tag="ON449_000359"
     CDS             complement(66263..68098)
                     /gene="tdc"
                     /locus_tag="ON449_000359"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3932981.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19415 a        14574 c        11695 g        22620 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacaatttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     9181 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     9241 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     9301 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     9361 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     9421 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     9481 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     9541 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     9601 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     9661 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     9721 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     9781 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     9841 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     9901 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     9961 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
    10021 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
    10081 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
    10141 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
    10201 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
    10261 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
    10321 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
    10381 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
    10441 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
    10501 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
    10561 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
    10621 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
    10681 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
    10741 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
    10801 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
    10861 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
    10921 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
    10981 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
    11041 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
    11101 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
    11161 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
    11221 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
    11281 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
    11341 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
    11401 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
    11461 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
    11521 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
    11581 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
    11641 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
    11701 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
    11761 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
    11821 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
    11881 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
    11941 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
    12001 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
    12061 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
    12121 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
    12181 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
    12241 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
    12301 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
    12361 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
    12421 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
    12481 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
    12541 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
    12601 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
    12661 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
    12721 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
    12781 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
    12841 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
    12901 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
    12961 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
    13021 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
    13081 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
    13141 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
    13201 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
    13261 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
    13321 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
    13381 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
    13441 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    13501 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    13561 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    13621 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    13681 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    13741 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    13801 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    13861 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    13921 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    13981 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    14041 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    14101 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    14161 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    14221 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    14281 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    14341 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    14401 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    14461 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    14521 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    14581 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    14641 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    14701 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    14761 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    14821 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    14881 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    14941 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    15001 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    15061 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    15121 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    15181 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    15241 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    15301 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    15361 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15421 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    15481 cttctcctgt ttttggtttg gttggcttat tatcgagaag tagctactgg gagtctattt
    15541 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    15601 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    15661 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    15721 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    15781 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    15841 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    15901 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    15961 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    16021 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    16081 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    16141 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    16201 atttcactgt ttttattttt ctttacagcc actgatatta tgagctaatt tgagataatc
    16261 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    16321 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    16381 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    16441 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    16501 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    16561 gatcaccttt tgcaaagagc tggcgttctt ttttattctc cttcctattt gtaactgctg
    16621 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    16681 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    16741 ctcctgtttc tccaacccgc aactgatatt taggacgaag tgcttcccga tcgaaagcca
    16801 tagcagccgg cacaaaatct tcatgatctc cggcgaattt cttgatttct ccataatggg
    16861 tcgtggcaat gatcaatgcc ccttgctcat acatagattc catgattgcg atcgctagtg
    16921 cagccccttc atttggctct gttccactgc caatctcatc taaaagtacc aacgtattcc
    16981 tttttatctt tttcaaaata gcagcgatgt tttgcatatg accagaaaat gtactcaacg
    17041 cattttctaa attttggtgg tcaccgacat cgacgaaaat ctcatcaaag acagcaagct
    17101 ctgtcccttc ttttgccgga acttgcatac cgaacatcgc catcaaagtc aataaaccga
    17161 ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc ggtgataatc aagcctcgat
    17221 agtctttacc gatttcaaca tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc
    17281 cttgcttgat aatgatatgt tccgatttat tgattctagg tgtgcttcca ttgatttctc
    17341 ggctaaattt tgcccgagca aagatgatat ctaacaccgt gatcgtttcg atcaataaat
    17401 cgatcacttc ttcgttttct gcgatagcac cagtcaatgc cgccaggact tgatattctt
    17461 cactgatttc ttcagctttt aacaactgat agtgttcatt caatttagaa atcacagttg
    17521 gttcaataaa aaccgtcgtt cttttatttg attcatcgat aatcgttcca tctattttat
    17581 ttttatagct ggctttgata ggaatcgtat agtattcgcc tttttgaaca atcattgctt
    17641 cttggatcat ttctttgttt ttcggatgac gcaagaattt cagtaatttt gactggattt
    17701 ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac
    17761 tgactttatt attttttatt ttctggtcaa tcaactcttc tacatttatc aaatcaggaa
    17821 gatgtttgct ataagcaaac aacaagggtg tctggtattg attcttatca aaaaactttg
    17881 tgatcatccg actgcttcta agaaaatccg catattcgat caaatcggtt ggctgcagaa
    17941 tcaacccttt tttgacttgt tctgtcagtg catcgatcct cggcaacccc ataaatggaa
    18001 catgctgact gctttctaag atcaagcgtg cttctcttgt ttcttcttga cgatccaaga
    18061 cagtttgtag gtttgtcgag accgtcatat cgctgattcg ttttttgcta tagtttccaa
    18121 ttgctcttgc ttggatttcc tttttgatcc gatcaaattg taattgacta atcatttcgt
    18181 tgtccatatt ctctcctttt cttgaagaga tacctgtccc aaaataaata agggcctaga
    18241 gataaataaa aggatagaac acagcttaaa aaagtcatgt cctatcctta ttagaataca
    18301 aagatattgt tttcctgaca ggtaccgttt cataagcaca ccaaataaac tttgtttatt
    18361 ggctattcga cttactgatt agataccagc tacactcaca aaacataccc cattcttcta
    18421 ggtcttattt attttccact taactatagc aagacacatt ttggttgtca aactttagta
    18481 tgttttatag atttacacca ttaatgaata gacacctacc gcgataattg ctccgatcat
    18541 tggacctgcg attggtaccc atgaatatgc ccaatctgaa gttcctttat tagctattgg
    18601 caataccgca tgtgctaatc gaggaccaaa gtcccgagca gggttgattg catatcctgt
    18661 tggccctcct aatgaaagac cgatggctaa gatcaatcca cctacagcga atacgttcgt
    18721 tccaggtgca aattcgtttt gtccaaaagc tagcaaccca agaactaata caaatgtacc
    18781 aattaattct gtgatcacat ttgctggata attccgtata gcaggacctg ttgcaaacgt
    18841 tcctaaaatc gcgctttcat ctttcgttgc attccaatgt ggcaaataag aaagccaaac
    18901 aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt
    18961 ccaactaatt gcccctgtca tcgccatagc tagagagact gcaggattca aatgagctgg
    19021 gcccatgaat ccagaaatat agacagccat tgttacagcg agtccccaac caaatgcgat
    19081 gacaatccag ccagaagcct gtgctttact tttgtttaaa ttcacagccg cacacacacc
    19141 atctccaagc aaaactagga tcagtgtccc aataaactct cccatgattt gagtcatatc
    19201 actgttcatt tatttctccc cctttagttc ccgcagatct gattctgcaa tcaatgcttc
    19261 taaacgttta gtctgttgcg ccttctcctc ttctgtccat tcaaaatatc cagcaattgc
    19321 atcgatcaca ggttgcttga tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt
    19381 acgacgaatg agataatcgc ctggggccaa aaccatttct tcttctaaac cataacgtaa
    19441 ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa
    19501 gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa gtagcatctt cttcctctaa
    19561 accggctgct actccttctt ttgctaattc tgtcactgtt tcttctaatt tagttggatc
    19621 gaagtttcca cctgaaatct gatatttttt agagtcgatt tctttcgtct ctattccgta
    19681 ttcttctttt aataattggc gtatcaatcg caaagctcct tctgccatct tacggtaatc
    19741 ggtgattttc ccaccagata aagtcagcaa accatctggt tctctttcta aagaactacc
    19801 tctggaaatc gtagaaggtg ctttttcatc acggctgttt tccaaatggt tcaacacgtc
    19861 ttctacttca gcacgagaaa ctttgttttc cttatattca cttactgtat cgacaacttt
    19921 attgaagctc ttgtctgaaa tcgatccatt atctccacca ttgtaatcag aaccagaatt
    19981 accaatcaac agtggacgaa gtcctgccca gcttgcttcg atatcattca atgtgatatt
    20041 tgcttctgga tagcgatgat tgatcacatc caatagataa tctacatctt cttgtgtgac
    20101 tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt
    20161 ttctcttgga atagcaaaaa ccatccgccc atcttgcttt cctgtatcga agtatgtggg
    20221 ttgcggtaca ggcagttttt tcgcatctac aactaaatgg atccctttag ttggacgcat
    20281 tttaggagag actggacgcg taaaattcaa gtttcgtact ttatctaccc aaggaccact
    20341 cgtattgatc actaattttg ctttgatttc gatcacttca tctgtcagca ggtcacgagc
    20401 tttgacgcca acgatttgat cgccatcata taaaaagcca accgctttca ttttacttac
    20461 tagataagcc ccatcttcgg cagccttttt gatattatca atcactaaac gggcatcatt
    20521 gttgcggaaa tctagataga cacccgcgcc ttttagccct tcttttttca aaaacggttc
    20581 tctttccaat acttcttctg gtgtcagcgt ataattctca tatttggttc ctgtcacatt
    20641 tgccagtttg tcataaaggt ccattgctac tttgaccgag aacatattga aggttgttgc
    20701 tccttcgccc tcatagattg gcaaaagcat tggatctggt tttgggatgt gtggggcaat
    20761 actttgtacg actgcacgtt caccaactgt gtcagctact acttccacat caaatgtctt
    20821 cagataacga ataccgccat gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa
    20881 atcctgcatt tcgatcaatc ctgttttgat tcctgatgct gctgactgga ctgccacacc
    20941 ggcaccagtg attccgccac cgatgatcaa cagatccaat tcttcttgct gcattttttg
    21001 aatgctatct tgtcttgtct tgtttgaaaa catatggatt tcctccttat tcaaatgctt
    21061 gggtagcttt tactgctttt ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca
    21121 tttgaacttc aaatagcttg ccagcttcat aaaattcgcg gatctcgtct gtatctttcc
    21181 aataaccgac agctaatcct gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt
    21241 ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa ttgcatcaga tattcattgt
    21301 ttgctgcacc gccatctact ttcaatacag gaatcttgat tccagtgtca tcttgcatcg
    21361 tatcgatgat atctctcact tgataagcaa tcgactgtaa agtagcttta atgatatctt
    21421 cttttgtcgt accacgtgtc aagccaaaca tagcccctcg tgctgcctga tcccaataag
    21481 gagcacctag cccaacgaat gcaggaacaa cataaacctc atcttcgctt gtagagcgtt
    21541 tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg aagtccatct ctcagccatt
    21601 gaatcgaaga accagccaca aaaatacttc cttctaaagc atagtaaacc tttccattta
    21661 tgccgtaacc aattgtcgtc aataaattat ttttggataa ttgcggtttt tcaccggtat
    21721 tcatgacaat aaatgaacct gtaccataag tattcttgac catgcctggt tcgaatgcca
    21781 tttgtccgaa taatgcggct tgctggtctc ctgccatccc tgaaattggt gcttcgcttc
    21841 cataaaaatg ataaccttgc gtataaccat aaacttctga gttagaagtg acttttggta
    21901 acatcactcg aggaatattt agtaaatcta aaatctcctg atcccaatct agctgatgga
    21961 tattgaacaa catcgtccga ctggcattcg agtaatcagt aacatgtgct tgtccatcgg
    22021 tcagtttcca tactaaccaa gaatcgatcg ttccaaacat caattcgcct ttttcagctc
    22081 gttcttgcgc gccttctaca tgatcaagaa tccagcgtat cttcgttgct gagaaatagg
    22141 catcgatcac taaccctgtt ttttcgtgga aaaattcttg atagccttca ttttttaagt
    22201 tatctgcaat tccagctgac tgtctggatt gccaaacaac tgcatgatag attggacgtc
    22261 ctgtatcttt ttcccatatt actgttgttt cacgctggtt cgttatgcca atcccagcaa
    22321 tctgatttgg tttgatccct gattcgatga acgctccggc aataactgac tgtacagaat
    22381 tccagatttc atttgcatca tgttccaccc aactttcttg cgggaaatat tgcgtgaact
    22441 ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa gataattgct cgtgagctag
    22501 ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt cattgtgatt ccctccatta
    22561 tatgcaaacg ctttctttaa ctactcttat catactactg ttttaaaaaa agaaactgtc
    22621 aaaattttat tctttttttc tttttttgac agttttttat tatgaagcta ttttttaatc
    22681 aattcttttc ttttttcagc gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg
    22741 acgcaaattc tgacagataa tattcacact gttttttgtt tctctggcaa aatgtgatca
    22801 ccaaatcata ttctttacct tcttcataac ttccaatctc aataccgccg attcccctca
    22861 gttctctgat cagatattgt tgaaaagcaa tacgataaat cggcagactt tctaaatcga
    22921 ttccaatcac tactggtttt gcaatataaa aatcgatcat caaaagaaca ttggcatact
    22981 ggatcatcag gtaactaagc tcactatctt cagctctttt cctcggcaat tgctgcaccg
    23041 ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg cccgagcatt ttctgctgct
    23101 gttccaaaag ccgatcacgc tcatagactt cgatctttcc aacaaagaaa aaccattcat
    23161 tgttgatctg ggaaatttgg tagcctactg ccttttcttc tttgattttc aaacgacgag
    23221 gacgataatg ccataacatc gtttcccgaa tgtaagtatc taaaacagcg gttggtaact
    23281 ttttgctccg tgtcaaatca tactgataaa atatttcttc gtctactatc gaaaaagaaa
    23341 caaaaaagct gtaaaacatc attgtttcgt atacattcag ttctgctgct gttcgactaa
    23401 gatgcatcac gatgattgga tcgattgctt gataaagccg gtccgactga tagagtaatt
    23461 tcttttcttt caaagttgcg tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc
    23521 cgagccagca agcaatctgt gcttctgctg atgccgtaaa cgttgtttct aatacacggt
    23581 ccaatcctag aatgaacggt cttttttctg gtgtattttg caaaaaaagc ggtcgctgat
    23641 cttccgggat atattggaat agttcataat aaaaatatcg tatctgtagc tcttctccgt
    23701 ataattgacc gtttttgatt tgtaatccaa attcctctaa gagctggttc aattctttga
    23761 tctttcgaaa aacagaagac tcactgatat taaacgctgt cgctaatcga acgatggata
    23821 cttgctgatg ttctaataaa tagacgagca tcttaaactt caagctttcc tgcaataaaa
    23881 acaatagaat tttttccaaa cttacatctt cgtccaacac aagcagcgcc tgatactcat
    23941 ttcgctgtac ttctactttt ttttccatca ttcggccaat caattcaata tcttccaaat
    24001 attgatcaaa cgaggctttt gacagttgga catgctctct caaaccattg atagaggctg
    24061 ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg taactgctct tttctttcta
    24121 ataatgcttc aatcttcata ggcagtattc gccacctttg accacaccat atctggcaca
    24181 cggtattctg gctggaaaaa tagctcatgt tcttctaact gttcaaacgt ccatccttct
    24241 tctactgcaa ttgtcagttg gtcaagaagg tggaacatct cttttttgct ttggagctgt
    24301 gctccaagaa tctttttttc agaatcaaat agaaatttga gtttaacttt gctttgattt
    24361 attgctgtaa gcttgtagtc tacgtatcct gtttcaattt tttggggata aaaaatgctc
    24421 tcttcttctg tcaaacctac gcttcctaag aaatagccaa accactgatt gccgactgga
    24481 cgataggttc cctgatcctt tgtctgtgag ccactaattg tcttgctgac atttcgtgct
    24541 gtacggacag cgttatttac tagagaaaca tacatttttt catttgttgg acgaaaagtt
    24601 acttggatcg catcgccaat cgcataaacg tctttttgag acgtttgcaa gtagtcatct
    24661 acccagatcg ttccatcatc attcaatgtt aattgttctt cccacatctg attgtcgggg
    24721 cgagaatgat tagccagcaa aacaagatca tagttctcct ttcttttttc cgtctctaca
    24781 atagcggttt cattttcggt caaagacaca acttgttctt ctaaaaaaag tgaaagccct
    24841 tgatttttca tttcctttac taacggttct gtcatttcag gatctagata acgaaaaaga
    24901 attgactttc ggctttcgta aagatggatt tgtttttttc ttgatgccaa cccttcagct
    24961 atttcgattc ctgcttgtcc tgcaccaata attgcaattt tctttgcatt catgatcttt
    25021 tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt gaataagcgt tgattctgca
    25081 gaaatatttc gaaacttttg atttgaacca gtcgctagaa tcaaacgatc aaaatccaat
    25141 ttttttcctc ctgctaaaac gacttttttt tctttcaatc ctattactgt cttttctgtg
    25201 taaacatgga ttcgatagcg gtcagtcagt tcttctttcg tgatccaaga aagttgtttt
    25261 tcactggtaa cctttccttt taatatcaaa ttgaacccgc tgggaatcca tcctaacttc
    25321 gattgctttt caatcaaata tatttcactt gttggcatca attggcgaag ataaatagcc
    25381 gcatgaatcc ctgcaaacga cccgccgaca attacaatgt tcatctgcat tcctctttct
    25441 tataaggtgt tctactgcta acttctcaat cagaaaacac agtattcata actcgctttc
    25501 aaccttatga tacatagcga tgcagcagaa tcaaccctct ccatcatacc gtgttctagg
    25561 tctgcttctc aataatgtac tagtggacct aattgaagag tatcatttct gaagagatgt
    25621 ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat
    25681 aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg taatgtaata gttagaatag
    25741 acacatcatt tatttttaat tctttaatgc ttcaacaact gcttcgccta attgatgagc
    25801 agattgagga ttttgccctg taagaagccg cccatcaatt tgaaccactt ctgaaaaagc
    25861 tgctccagac tgatagtttg ctcctttttc tttcaaagca tcttctgcta agaatggtac
    25921 ttcattcgtt gtaccatttg cttcctcttc ttcattactg aacccagtca ctgtacgtcc
    25981 agctaaaata gattttccag actcatctgt aactggcaac aatcctacaa caccgtgaca
    26041 aacagctgag accagccctt catttcgata gatttgtgaa gcgactttcc caagatctat
    26101 tgcttctgga aaatcccaca ttgttccgtg gccaccagca taataaaccg ctgcataatc
    26161 atttgcatcc acatcatcag gacgtaaggt atttcccaag gcccgatttc tgaaatcttc
    26221 atcttcataa taggcccaat cgatttcatc catattcaaa acactttgcg gttctaaagg
    26281 tacatatccg cctttaggac tgacaaaatc tgcatcgatc ccatttttta ctaatacatc
    26341 ataaaaatga gtcagttcac ttaaccacaa acctgtagga cgctcgatct tttcgtactt
    26401 ttctacattt gttaatacga tcaatacttt cattttccca tcctctttct ataaaaattg
    26461 cgttattgat aacgtgcttt caagaaatta tataatgggc ttggcaaata aagctcttta
    26521 tatgcttctt caaaaggttt cgttacaaat tcatcctcac ggttttccaa taatttctct
    26581 gcccagtcag gatcgacgat catttgctgt ccaattgctg caagttctgc atatttcaat
    26641 acctgctcta catcttcgcg attgcggatc ccaccgacac caactaaagg gatctttcct
    26701 tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt tgtatttatc agaacgagct
    26761 attcgatcat acatatttaa tgaaacatgc agataatcta gttttgactc acgtaatcgt
    26821 tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg tttcatattc ttctggtgag
    26881 aaacgatacc ctacgatgaa tggtttttta gcatattgtt caaccgctga gaaaaccgtt
    26941 gttaataatt cttcaataaa acgataacga ttttccaagc taccgcccca ttgatcctct
    27001 cgtctattcg aatgcggaga gaaaaattgt tggatcaaat acgtatttgc tccatgcaac
    27061 tcgattccat caaatcctga ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt
    27121 gtttcaacga tttcttcttc actcattgct cgtggtgttt ccgcatcagg acgttttgct
    27181 gcaactgcac ttgcagatac aggctgttct ccagataaag ttgctcgact tgtcattcgt
    27241 ccaccgtgga aaatctgaat aatcgctttt gcgccttctc cttggattgc tttcgctagc
    27301 tcacttaatc gaggaatcat ttcatcatga gcgatgctga gttcacctgg ccagcctttt
    27361 ccgatatctt ggacatttgc agcaccagta ataacagcac caagaccttt agaacgttgt
    27421 ttataataag tgatctcatc ttcggtgatc actccattga aaaagctgag ttgtgtcgtc
    27481 ataggcgaca gcatcaatcg gttcttcaat tgaactcctg aaggtaaagt aattgattga
    27541 tcaaattgtg acattgtcat tcctcccata attcgctctg attaacctat cgagaatcat
    27601 ctatcattaa accactcgat tagttgtaca tgcaactaat ttgcttaaaa aattttattt
    27661 tttttgtaac ttatctgttg catcattcat tgtctgggct aattctttac ttgcttcatt
    27721 tcccaaaact gctttgtatg actgatgaag ttcttcccag ctaccatcga cttccttagc
    27781 aaattggcgt ccttgttctg taagataaac taatgacaag cgaccttcgc taactgtttt
    27841 gacgtatcct ttatattcta atttttcaac aaaacgagca atcgttgatg gggcggaata
    27901 gagcatttgt gcaatatctt tttgtggtat tccatctttt tcttttaata tcaataaaat
    27961 aaaaccataa gttggtgcca gacctgtcct tctaaacgct tcatcagcta atttattcaa
    28021 catgcgatct agctttttca cgctaaagaa aagacattca tctaacgctt tttcaattgg
    28081 cttttccatt tcatcccctc cattggcgta catatcatag tatgattttt attttttgtc
    28141 aaaaaacacg gaataatgtc cgcctcctct gtctagattt gaaaaaaaga agtagccatc
    28201 ttcaaagaat ttcggcttgt tttcgacaaa actccttctg gaacaccgtg aatcagaggt
    28261 tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt tgtgaaaatg gctttccaca
    28321 tttttgcata aatttggctt aataccgaaa aactggcttt tgaacaccgt ttatatcaga
    28381 ggttgtgaaa aagccgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    28441 atattttgtc gttttttgag ctaatgccga tggtcagctg attgtacacc gtttattgag
    28501 aggtcgtgag cctgccgttg aactccgagt cacaaggagc aatttcaaaa aacagcttct
    28561 catgttttat gactatggtg acttgtgatc gaggagttgg caggagaata ctgtttattc
    28621 gtaaagaagg ggctgtgaca agacttttgt cacagtccct tatttccttt tattatagga
    28681 tttcgccatt tgattggatg acttgtctgt accaatcaaa tgattttttc tttaaccgtt
    28741 tctttgtgcc attcccttcg tcatcttgat caacatagat atatccataa cgttttgaca
    28801 tttgtgaagt agaagcgctg acgatatcaa tgcagcccca cgacgtataa ccaaataaat
    28861 ctactccatc tttgatagct tccttcatct gctcaatatg ctgtttcaaa tagtcaatgc
    28921 ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc tatagctccg agtccgtttt
    28981 cagctatgaa cagtggtttt tgatacctat catataattg attcaaagca attctcaaac
    29041 ccttaggatc aatctgccag ccccattcac ttgatggaag atacggattt ttaattcctc
    29101 cagttattaa gtttccttgg accccttccc aattttctgg atgagcactg actgcactag
    29161 acatgtagta gctaaaagaa acaaaatcaa cagggtaggc tgctaagatt tcttcatcac
    29221 ctacttctat ttttaagttg acaccttttt cttcaaacat tcgttgcgta tacgctggat
    29281 aatatcctct tgcttgaaca tcactaaaga acaaagaagt tctttgctgc tcaattgttt
    29341 tttgaatatc atccggatca caagaatacg gaacaagaag ctgatagctg atcatacaac
    29401 cgacttgtga acctttgata atttcatgac aagcttttgt agctaaagca cttgctataa
    29461 actgatggtg tgcaccttgg tatttcgctt gttccaaatg gggatggttt tcttcaatta
    29521 ctcctacgct aacaaaaggg tgatgtttta cacagttgat ctcattgaaa gtcatccaat
    29581 attttacttt atttttatag cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa
    29641 taagctttcg attaggccat ccaccatatt ctgtaaccaa atataacggc atttcataat
    29701 gggaaagaga aacgactggc tcaatcccat attttagaca ttcgtcaaac acttgatcat
    29761 aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc
    29821 atgcgataga catccgatag catttaaaac ccatttcagc aaataaacgg atgtcttctt
    29881 taaagtgatg atagaaatca tttccgtgtc ttttaggata ataatacttg ctgttgggat
    29941 cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca ttgatctgtc catttttctt
    30001 taggtaaatc gctgtcgaat gtatacacat cagacacaga taatcctttt tcatctacat
    30061 cccatgcacc ttcacattga ttggccgctg tcgctccgcc ccataaaaat ccttctggaa
    30121 attttttcat atcgatcccc ctaaacttgc tagttttctt gtttgattat ttttcttatg
    30181 actagtaatg atatttttga atctactact atttgtaaca ataaccattg tggtcggatc
    30241 atatccctct ttgattaatt cttgcaaatc tacttccatc aataaatcgc ctttacgtac
    30301 tttttttcca tcagatactg cactttgaaa gtatttgcca tttaaattca ctgtatcaat
    30361 accgatatgg atcagtatct ctgctccttc ttcactgact accccaattg catgtttcgt
    30421 tggaaaaact gttgaaactt ccccgtttac aggactgtag atttttccgt ccgttggaat
    30481 gatcgcaaac ccttctccta aagatccact agcaaatgtt ggatcgttta cttcagataa
    30541 atcaattatt tcacctgctg caggagtact taacactata tcctttgagg attcttcttc
    30601 gatttcatcc ttgtagccga aaagaaaagt tagcatacag gaactgatca tcgctaagaa
    30661 ataactagca actagccaaa caaatccttc accaatataa atcgggaaag acaaaatact
    30721 tcttgtagca actgccaaag caccggctcc tgcggcacca gctactccac caaatatagc
    30781 tccacatata caagcaatgt aaaatggtcg tttatattta agagtcacac cataaacagc
    30841 aggttcagtg attccaaata atgctgtgat aaatgcagat aaagataaac tttttacttt
    30901 cttatttttc gttttcaaaa ataccccaag tactgctcct gcttgcgcag ctacggcagt
    30961 catacatgct ggacctagcg tatcacgacc gtagaaagaa agattgttgg tcattactgg
    31021 tacaagtccc caatgtaacc caaaaatcac taacacttgc caaaatccgc cgataacagc
    31081 accagataaa acagggctaa agttataaac agctaaatat ccagctgcaa tcagctgact
    31141 tgcataatct gcaattggac ctacaactaa taaagtaatt gggaccatca cgatgataca
    31201 aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc
    31261 aatataagaa agaacccata cagctaaaat tgctggaata accgtacttg tataccttgc
    31321 tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta tcactatatg cactgacaat
    31381 cgtaggataa acgagagctg ctgcaataac catagcgatg aaacgatctg tattgaactt
    31441 ttttgcagca gtatatgcca aaaatatagg caagaaatag aaaaatgcat cagctgctgc
    31501 attcaaaatg atataagttc ctgattcagt agttaataaa ttcaaactcg tacataaggc
    31561 gagtactcct ttgatcaaac cacttcctgc caagacgcct aaagtcgggg caaaaacagc
    31621 ggcgatcatg tctataaaag agtttaaaat gccttttttc tcagttcctc tctttccact
    31681 agatgcatca tcttctagtt tcatttgttt ggataatacg tcatacactt catttactgc
    31741 atttcctact acgacttgaa acattcctcc agcttcgact acagtgatca caccttctag
    31801 ttgctctacc ttctccttat ctgccttctt attatctttt agggtaaagc gtaaccttgt
    31861 tgcacaatgg taaacattgc tgacattttc ttcgcctccg acaaatcgga caatatcttt
    31921 cgctaattgt gaatagtcca tttttacttc ccccttgtta attaatgccg tagcaatcct
    31981 tcttacaaga taagtatagt tcttagaaaa cggtttctcc attcaattag taccccttat
    32041 aatagcgaac gaaactgtac aatagtgtgt atggtttact tagccgagca aatcactcac
    32101 tgcttgaaag aaaacaagta tatcgtttct cttatagtca aaggagacaa aaaaaacacg
    32161 cttgatcttc ctttgctgat gtataagtgg tatctcaagc tgatactctg tgatttttgt
    32221 ttctacttgg cttttgataa gacagcacaa caaagtctcg gcatttaacg gaagcataac
    32281 taggtcagaa ctaggagaga ttagttgctg aatggaatct atctttttat ggatttttat
    32341 ttgattttct tctttgtagt aatgatcaag cagttttgtt ttcttttttt cttcaactat
    32401 ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat tcaaactcat ctagaacact
    32461 aggtagataa atcaaatcat cccactcaaa aataagctct tgctcagtag tcaaaattgc
    32521 atcatattcc gttattttgt ttgattgatt tttgaaaaga tacactggtt ctatccggca
    32581 ttctttgatc cacatgttca tactttgttg aatctgtttt tttagtgtat tcaaaatact
    32641 aattggctgg ttactaacaa tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc
    32701 ggcaagatac aaaacaaaat aagcgctttc agctaaattg ggacgtaatt gaaaatgtat
    32761 tgggaagaaa cgccgaatta aataaatttc taaaggataa cgggagatga tcttttgtac
    32821 aaaatgattc atggtattat gtccggcatc catcctcgtt ttcatttgtt tgatatgggt
    32881 tagcaaaaat tctgtttcat ggaacaataa cgaaacagga agatctaaaa aacgaataat
    32941 cgtttgctca aatgctctta gattcttctc tatttctttg tttttttcat cattaatcat
    33001 caaataattt aactctaata gtcgattttc aattgcttgt ttttcttgat tggaaaaaga
    33061 atagggtagt tctgatgata acttttttgt gagggtctct accatcggaa taaataatgt
    33121 atacctgttt gttttttcta aaagttcttc tcttcgaaga atggtcaaaa cgagataccg
    33181 agaaaatcgt atgaagtcct ctcctgaaac aatataatta tatgatataa gaacctcttt
    33241 taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa
    33301 ccttgcgtgt tggatcaccg tttctgttcc aacaagagat aagaaaattc tttttgaatt
    33361 ttcttcagca atgattttta gtccttttgt cgacgaaact tccagttcta atttaggatt
    33421 gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa
    33481 gaaaatgtct gctatctttt gcatactgac ataccctttt tgaaacaata agtacgtcac
    33541 aatagttgaa gctcggaaat ccatgctgga atagatttta tgtttcgtca cctcttgtat
    33601 gatttgctgt ttagtttcag attcaattga ttctaaaaaa taacctgaac gtttatttga
    33661 ctggataatg attttcttct cgtagatagc aagcatattt ttgatttcag tttggatcgt
    33721 tttcttgttg gtacctaaag cttcagctaa ttcttcgctt gttatccaat gattcttatt
    33781 tcttaatact acaagaattt ggagttgtcg tttatttaag tccatttgct tacacctcct
    33841 ctttgttata aacatacagg aaaaacctct caaatgacaa tagtcacttg cagaggttta
    33901 aaaatcattt gaagaaagat ctatattcaa ggtttttacc attagactgg ataatttgtt
    33961 tataatattc aaaactatct tttttcaatc tcctaaagct tccgctccca tcattttgtc
    34021 tatccacata aataaagcca tatcgcttcc tcatttcccc tgttcctgcc gatacgatat
    34081 cgattggtcc ccaccataag taaccaatga tttggcaacc atcttttatt gcctcagcaa
    34141 tttcctgaag atgtattttt agatactcac aacgttttgg atcgtggatt cgattcaatt
    34201 catctggtgc ttcatctagt ccaatgccat tttcaactat gaataatggt aattcataac
    34261 ggtctgtcaa ttcattcatg acataacgaa accctagagg atcaattggc caactccaag
    34321 gttcgggaga tttttccttc aaaaagggat tatcgaaacc atttgctccg ccagtatcta
    34381 cgcgcatttc tgcttctttt ttatagacag cgctacgata ataactaaat gcaatataat
    34441 ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa cattactggt gcacagtctt
    34501 cttccttcca tgtcctgtaa atataagctg gataatgccc tcggcacatg acatcggaga
    34561 aaaaccatga attgcgttta aattgcagtg aaccaaatac attctctgga tcacagtcat
    34621 atgcataagt cgctaatgca gaaaagctac acataatacc catcttagca tttggatcga
    34681 ttttctttaa taattgagta gcacgggcgt tcgctagaaa catatgatga caggcttgat
    34741 aaatcacttt ttttgttagt gtagcaatgg gttcaagttt atctctagca gggaatgtaa
    34801 ccaatccagc agctgcataa ggcatacgaa aagcattatt gatctcatta aaggtcatcc
    34861 aatatttcac tttcccttgg tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt
    34921 cgattaattt tcgattttgc caccccccat actctcctac caatgctaag ggggtttcat
    34981 aatgactaag agtaattact ggctcgatat catatcgatt taatgtattg attagtctgt
    35041 cataaaatac gagaccagcc tcatttggct tctgttcgtc tcctcttgga aatattcgtg
    35101 cccatgagat gcttgttcga aaagctttta aacctaactc cttcagtaaa cgaatatcct
    35161 cttcaaatcg atgataaaaa tcaatcgctt catgagaaag ataatggagt gattcgtcta
    35221 aagcaatctc ccaaatcttt ttttcttcat tccactgaat actaggatgc tggttcatga
    35281 tcccgataag cgtatctgtc acattaggta atttgccgtc ttccaaccat gcaccttcac
    35341 attgattagc agctactgct ccgccccata aaaaattttc tggaaatatt tcctgatcca
    35401 taactatctc tcctcgctat caacaatttc ttacttgaag tatagctcga ttcatcaaaa
    35461 caagatattc taactatttc ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt
    35521 ttaatattta tatataacaa atacataaga cgatccccga aagaaaacac tcgttaacca
    35581 tctaagcaaa cgatttattt tacttccaac acaaatgctt cataaggtcg taaatcaact
    35641 ttgccgtctg gtaactgttt cagaggataa tttgacacaa taacccttaa agcttcacgt
    35701 ggaacagtga acgtctgctt ttcactggac atattacaaa taacgagcca ttgctggtct
    35761 tcatatatgc gagaataggc aaagacatgc tcaggcgtct tttccactaa ttcatagtct
    35821 ccccagacga tgatgtcatg ttttctacgc aaatcgatca atttcttgta catataaaaa
    35881 acagaatctt gatctgctaa cgcttcttct gcattgattt ctttataatt tggattgact
    35941 cgaagccatg gaattccagt agtaaaccca gcatgttcac gggcgttcca ttgcataggc
    36001 gtcctagcat tgtctcttcc tttcgcattg attgattcca agatttcctc ttttgtaaat
    36061 ccagaagaaa tcctttcgtt atacatattg attgtctcaa tatcttttgc ttccgcgata
    36121 tcatcgattg ggcaattcgt catgcctaac tcttctcctt gatagatata aggggtgcct
    36181 ttcatcatat ggagcaagat agcaaacatc ttggcacttt cttttcgata cacatcgtca
    36241 tttccccaac gagaaacaat tcgcggaaga tcgtgattat tccaaaataa actattccat
    36301 ccttcatcac ctaaagatgt ttgccatttg gataatactt ttttcagttc agagatactc
    36361 aagggtttca aatcccattt gtctttgcct tcttgctgat ctaatcctat gtgttcgaat
    36421 tggaagacca tggataattc atttctttta ggattggaat aaagtttagc gatttcggta
    36481 gttgctcccc atgtttcgcc aacagtcatc agatttttgt caccaaaagt tgcttgattc
    36541 atttcctgaa gatactcatg cagtttaggt ccattacttg tgatttcttt atccggttct
    36601 ttgccaatca tatcgatcac gtccatacga aagcccccaa tacctttatc gatccagaaa
    36661 ttcatcattt gataaatctc ttggcgaacc tgtttatttt tccaattcaa atcaggttga
    36721 cgcttgctat acaagtgcag aaaatattgc cccgtttttt catccaactg ccaagcagaa
    36781 ccactgaata ttgagcgcaa tctattaggc tcttttccat tgaccggatc acgccaaata
    36841 taatagtccc gataaggatt ttctttattt ttacgggcct caatgaacca cggatgttca
    36901 tcagaagtat gattcaccac cagatccatg atgatacgga tatttctttt ctttgcttct
    36961 tcaatcagtt cttccatttc ttccatcgta ccgaattctg ccatgattgc ttgataatta
    37021 ctgatatcat acccattgtc atcattcgga gactcgtata ctggagaaag ccaaattgca
    37081 tcaataccta aatacgccag ataatccaac ctggaaataa tcccccgcaa atctccaata
    37141 ccatcttgat ttgaatcttg aaaacttctt ggatagattt gataaacaac tgaatggtgc
    37201 caccattttt gttccatgat tttcctccta taaatggatg tttctatttt ttgcttaatc
    37261 atttatttta gtctctctca ctaaaaattg taacgctttc aaatagctat tgcaccttgt
    37321 tatcggtaac agaataaaac aaaaaaacag ctgaggaaat tccctcaact gttttttgaa
    37381 aggattgatt tatgcaggga agaatgctga tttcacaact tctttataaa cggtgttcac
    37441 tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttttcgtt
    37501 tttttctcta atactcaggc cagaacactg atttcacaac ctctttataa acggtgttca
    37561 ctcagctgtc cctcggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    37621 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    37681 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    37741 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    37801 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    37861 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    37921 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    37981 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    38041 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    38101 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    38161 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    38221 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    38281 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    38341 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    38401 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    38461 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    38521 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    38581 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    38641 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    38701 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    38761 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    38821 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    38881 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    38941 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    39001 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    39061 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    39121 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    39181 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    39241 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    39301 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    39361 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    39421 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    39481 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    39541 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    39601 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    39661 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    39721 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    39781 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    39841 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    39901 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    39961 tgctttcctc cacaaacttt ttttacaaaa aaaaaaacgg caatgaatgc cgctcttctt
    40021 atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact
    40081 agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg
    40141 tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt
    40201 cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc
    40261 atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa
    40321 tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc
    40381 tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt
    40441 gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg
    40501 tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt
    40561 ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa
    40621 ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa
    40681 gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt
    40741 catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga
    40801 tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa
    40861 gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct
    40921 tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt
    40981 tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc
    41041 gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa
    41101 tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag
    41161 taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata
    41221 ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc
    41281 acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg
    41341 ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa
    41401 agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt
    41461 tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa
    41521 cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct
    41581 actttgatca tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact
    41641 aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa
    41701 tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt
    41761 tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat tttcaataaa
    41821 aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag
    41881 ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc
    41941 gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat
    42001 tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat
    42061 actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag
    42121 tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag
    42181 acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga
    42241 ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat
    42301 gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac
    42361 tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt
    42421 ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct
    42481 tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga
    42541 ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa
    42601 aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc
    42661 ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc
    42721 tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa cggtgttctc
    42781 ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct gtttttttga
    42841 aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt aaacagcgtt
    42901 caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat tgatttcttt
    42961 ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta catcaaattc
    43021 atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct ttttgaatgc
    43081 ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac catagtgata
    43141 atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt accgacgttg
    43201 acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc cgtatctatc
    43261 tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg acacaatcac
    43321 tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat aaactttcgt
    43381 cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg catagtattc
    43441 cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta gtttattcat
    43501 aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact ttttcatctc
    43561 atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc ttatgcttga
    43621 caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac ttgttgattt
    43681 ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc tccgttccaa
    43741 cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct ttcacacctg
    43801 ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc agcaagggaa
    43861 tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca atagctaatg
    43921 catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta tataccttgt
    43981 cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt tctccttcta
    44041 cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg atattttctt
    44101 tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg attgcttctt
    44161 tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc atttcttctt
    44221 ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta gcggtatcga
    44281 cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa
    44341 gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct tctgctgttt
    44401 ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt ttatttgttt
    44461 cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc catgtagtaa
    44521 tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt atagaggctc
    44581 atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt ttctaaatat
    44641 tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg ttcataaagt
    44701 ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg tgtaatctct
    44761 gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc cagaactttt
    44821 ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac tttcaattaa
    44881 aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc tactgtatct
    44941 acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac tagcaaagcg
    45001 ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc tcggcgtccg
    45061 ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt tagcatacca
    45121 ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat cgtaccaatc
    45181 gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa aactggacaa
    45241 tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa tgccggcacg
    45301 atttcaccta acagtagacg tacaccagct agcaatacat acacacccac tacgaattgg
    45361 atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc agaagcgaat
    45421 ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac catgacagac
    45481 aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt
    45541 ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa gtacccgatc
    45601 gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat gatcggttgc
    45661 gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac caagatcaat
    45721 acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc catccataaa
    45781 attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa aataatattg
    45841 aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa atcattcatt
    45901 gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa tccttcagta
    45961 aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc actcaacact
    46021 tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga tttcttctgc
    46081 agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt caaaatattt
    46141 tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt atttagataa
    46201 ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt gaaacaatat
    46261 ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac tgcgaacgaa
    46321 aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa atatcacaat
    46381 ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac atctgtgctg
    46441 caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg ttcatctttg
    46501 ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt ttgaaatctt
    46561 cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc aattcaatta
    46621 gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa aaaataatct
    46681 ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta gctacgctga
    46741 ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg tgtttgccaa
    46801 tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca tagcgctctt
    46861 cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc tcccaagtag
    46921 tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac ataggctgga
    46981 tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac ttgccataga
    47041 tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga gtgaataact
    47101 ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt ttcaaaaagt
    47161 cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa agcggcttat
    47221 ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta tctactaatt
    47281 cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt tctttcagat
    47341 tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact gatttataaa
    47401 cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg acaagaaaag
    47461 ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga acaaaatgag
    47521 tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat ggattcatga
    47581 cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag agattttcga
    47641 ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca aattggatca
    47701 attggatcgt tagtaattga gcttgtaccc actcgattga attggagata tctctagcat
    47761 tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca ttgtccaatt
    47821 gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt atttctgtat
    47881 ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc cagatcaatg
    47941 taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac ttattggaat
    48001 agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag attttttgag
    48061 ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga
    48121 taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct
    48181 ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat
    48241 cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg
    48301 ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa
    48361 agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct
    48421 tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc
    48481 agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg aactcacttg
    48541 ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg
    48601 ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa
    48661 taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa
    48721 taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat
    48781 tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    48841 caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta tcgcagatgc
    48901 aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc ttgagcgata
    48961 agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag
    49021 gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac
    49081 tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat cggcttgcca
    49141 tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt
    49201 cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa
    49261 atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat
    49321 agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg
    49381 atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc
    49441 ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg ctttcttttc
    49501 tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct tttgtctttt
    49561 attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa
    49621 tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat aagaatctct
    49681 gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa atgaacctct
    49741 gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg
    49801 ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt
    49861 tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat agccttgcta
    49921 aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg tcttatttag
    49981 tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc tatcttcagc
    50041 atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg gttttcctga
    50101 caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact agcagcatga
    50161 gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac gcatgtgtca
    50221 tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata tgaatcatta
    50281 tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa tatcgcttct
    50341 tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt ggttttttta
    50401 tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag tattttcgat
    50461 aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa tagccccatt
    50521 gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat tgccttttcc
    50581 gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc atgatgccat
    50641 gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt aagaaataaa
    50701 gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc aggttttgga
    50761 cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg ctgagtcact
    50821 gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta ttacttgctg
    50881 aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc aaatgcacaa
    50941 cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg cagccaacta
    51001 tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata cgtacgataa
    51061 gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac aatcattttg
    51121 gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg gatcgtattc
    51181 aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga acggacagaa
    51241 aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc caaatattga
    51301 tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact agcaacaaac
    51361 cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt ttgatagaac
    51421 agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac atatttttta
    51481 ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg gatctgcagc
    51541 atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt cagtaaaaag
    51601 cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca aagataagcc
    51661 gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa aaattcccaa
    51721 taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat aaacattggt
    51781 gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt tgcgctgtgg
    51841 ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac agctgatact
    51901 ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc tttctctctc
    51961 tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt ttcaatagaa
    52021 tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc cgagtcacta
    52081 cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga attggcaatt
    52141 caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt actgccggaa
    52201 tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat cctgataata
    52261 tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc tcttgtctat
    52321 acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca tgttttgaga
    52381 tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt agtatttctg
    52441 cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga acgacccatt
    52501 caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca tgtgcttgaa
    52561 tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt gtttcgacac
    52621 gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg tcatttgtat
    52681 tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca ttttgttctg
    52741 aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt tttgtataat
    52801 tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt tcagcaaccg
    52861 aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta atttctttat
    52921 tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca cctgaaatag
    52981 catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt gcataagcag
    53041 cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag ccgtatgctt
    53101 ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct ttatcttctt
    53161 cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca acttcaccgt
    53221 ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg atggcttctt
    53281 ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt ggatttttca
    53341 tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt tcatttcctt
    53401 ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct tcattgtaat
    53461 cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa taaaatgctg
    53521 agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca gcagctttga
    53581 ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc cgttgacgag
    53641 atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga acgcctgctt
    53701 cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt gcgattaaat
    53761 aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt cctggaatca
    53821 aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt tctgaaatag
    53881 ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt acaatgactg
    53941 gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca gcaacgattt
    54001 ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata gcgattacta
    54061 ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca ctttcaggga
    54121 cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca atcaattcac
    54181 gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca aaactatttt
    54241 tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc tggtgtagca
    54301 ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa tctcgttaat
    54361 acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac agtagattct
    54421 gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac tcctacatgg
    54481 attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc catggatctc
    54541 tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt cttccatctg
    54601 ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg aatttcattg
    54661 gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt gcagctaata
    54721 atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc acgatgccgg
    54781 ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt gtaacaccgc
    54841 caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac aaataagcaa
    54901 ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact ggtgcattgg
    54961 ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag actggttttt
    55021 tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc cctaatggat
    55081 catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg ccaggattat
    55141 gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg gtagaagagt
    55201 ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca tattgtcctg
    55261 cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca aaaaagacat
    55321 ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta tggatgcttt
    55381 caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat acccagttct
    55441 tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac aattcgatag
    55501 ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct gcgtagattc
    55561 ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga ggatctcgtg
    55621 tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct acccagtcac
    55681 cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg atcacgatac
    55741 catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg aaacctgtgt
    55801 acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt ggaatcaaat
    55861 cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat ttcattgtgc
    55921 cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag aaatcgatct
    55981 tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata ctagacatga
    56041 tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct gaaagaaaac
    56101 ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca atcagttttt
    56161 ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag ttgctgattt
    56221 tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt ggtttttcca
    56281 taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca tgttggtcta
    56341 gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc tttgtcaaag
    56401 tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc aagtccatca
    56461 tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga atctcttctg
    56521 cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca tcagatagat
    56581 ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag agacagtgag
    56641 atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg gcttctgcca
    56701 acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga caattttcat
    56761 ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc tcagcttcgc
    56821 ttctcgtcac acctactact acgcccattg cataaccgct tggataagct tctttcatta
    56881 agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat cgacaagcaa
    56941 cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga ataccgtttt
    57001 tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg ataaaaaccg
    57061 tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt tctaaacgtt
    57121 ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct tgtccaggaa
    57181 ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc actgctatct
    57241 tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa caaaaaggta
    57301 tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa agtattcgaa
    57361 acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc cttgagccgt
    57421 gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag cggcagaaac
    57481 attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac attagacaca
    57541 ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac ttccgtcata
    57601 ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt tatggaaaag
    57661 catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat taccgatcct
    57721 gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat catgaaagaa
    57781 cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca tctgcaatta
    57841 gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt ccgtcataca
    57901 ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac gacagatgat
    57961 cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt cccaaaagtt
    58021 tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga tttgcccttg
    58081 acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat caaaatgatt
    58141 gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg ggacaaaatt
    58201 cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt agaaaataag
    58261 ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    58321 taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg ttgatttaaa
    58381 acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta gatcaataga
    58441 gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc ctcgcaaaat
    58501 tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat ggtcacttgc
    58561 atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt ttttgctttt
    58621 ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg gagacaaatt
    58681 gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct tccatctctt
    58741 tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga atacaagtag
    58801 ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt tcaatccgcc
    58861 tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc cacctatttt
    58921 ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc agtaaatgct
    58981 ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt tattttctcc
    59041 gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa aatcattacc
    59101 gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca tacaaagata
    59161 accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt ttctgacctt
    59221 caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca agcaggaatt
    59281 agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt tttcaaacaa
    59341 tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc tgtccctgat
    59401 tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac taaaatcaat
    59461 cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc ttgtattctt
    59521 ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt cactcataac
    59581 aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt tttaagaaac
    59641 ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg ttggttcttt
    59701 atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca taccaacagc
    59761 acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga aggtatttat
    59821 cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg gaattacaac
    59881 aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt agaggtcctc
    59941 gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa aaaaacatct
    60001 ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat attgattgaa
    60061 gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata tgcacacttg
    60121 tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat caatgaacca
    60181 aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc tatcaaaccg
    60241 aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc gttcttgcag
    60301 caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga actaaccgat
    60361 ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc tctagattct
    60421 ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct caatcgtttg
    60481 actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt ctctagctgt
    60541 tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta caactaagaa
    60601 tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac ttactgtacc
    60661 gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag caaggatacc
    60721 taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg gcttggacaa
    60781 ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca gaagaatggt
    60841 ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa ccatgatcag
    60901 tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt gctttggatc
    60961 tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtt cataagataa
    61021 agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag gtagcgggcg
    61081 attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg gcaagaagaa
    61141 attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg cttttgcaat
    61201 cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc
    61261 tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat catttacgcc
    61321 gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa ttcgcagttt
    61381 atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa tcaactcttc
    61441 atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt ttaagccaat
    61501 ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt tcactgagat
    61561 ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg gatcgatcaa
    61621 tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga
    61681 atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa attctgcagc
    61741 tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa ataatacttc
    61801 tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg ttgccatata
    61861 tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta gattggctgt
    61921 ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt ctgttttctg
    61981 ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc aggcttcttc
    62041 ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg tcttatctga
    62101 acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga tgatcgcctt
    62161 ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag ctggcaatcc
    62221 tttgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat gccaatccat
    62281 accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa tgccttgaaa
    62341 aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt gttcgaccga
    62401 ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc
    62461 gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct ggtcgccgat
    62521 cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt ctcccgtcaa
    62581 aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg ctggaacgac
    62641 atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag ggattttttc
    62701 ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa
    62761 tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga tcactacgaa
    62821 taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag aggcaatgat
    62881 caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc gttttgtctg
    62941 ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc gttcttcttg
    63001 cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg tcaatctgta
    63061 ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag ggaagttttt
    63121 tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa atgtatctgg
    63181 gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa tacaaaccag
    63241 ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct gaaagcaatt
    63301 gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt ccgtttcttt
    63361 tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag aaaacaactc
    63421 gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa aaaatgtaga
    63481 caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg tacgcctttc
    63541 ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg atataaaatc
    63601 ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa aatcattaga
    63661 ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac taaaggactt
    63721 tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat cgttcctata
    63781 tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt cttttgcgga
    63841 tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact gaataaaacc
    63901 ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc gatctgtcca
    63961 aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa tcctataccc
    64021 aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga ttccaattca
    64081 ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc gctacctccg
    64141 cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat tgctgttaat
    64201 tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa aaatgcccac
    64261 tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact atttgctaaa
    64321 aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat caaataaacg
    64381 agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa taatcctgtt
    64441 ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt ccgtttcatt
    64501 gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga cttcctgttc
    64561 ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa tgacactgat
    64621 ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc cgacagaaaa
    64681 tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc tctttttctc
    64741 tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca atctcaaaac
    64801 tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat ttacgagcat
    64861 caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga ttgagataat
    64921 gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct tccaaaaatt
    64981 gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg ctggtcctga
    65041 ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa acagattcac
    65101 aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg cgatattatt
    65161 caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca aaatattgac
    65221 gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa ctggattttc
    65281 caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca tgatcggcgc
    65341 aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc atgaaaaact
    65401 gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc ctaataagag
    65461 catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc cgatttttgc
    65521 catatcaaat ttcaaattca aaaaagtgat gatccaataa acgactaaaa tgacaactaa
    65581 tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa acaacggagc
    65641 gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca gccatgagac
    65701 aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac cacctttttg
    65761 gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt acaataatac
    65821 tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg gaatatttcg
    65881 tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga tttgctttga
    65941 tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt tttgttttct
    66001 tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca aaacccagta
    66061 tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga aacgaacgat
    66121 ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat gaaaaacagg
    66181 gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg tctcttgtta
    66241 ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt tccattgctt
    66301 tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa ggagtcatga
    66361 tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc gttagaccca
    66421 ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat gtagtatgag
    66481 aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga tcgaacattt
    66541 tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg aatacccaat
    66601 tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt cctttaacag
    66661 taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt gaagccccaa
    66721 tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc cagactgcgg
    66781 cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata tctggagcct
    66841 ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc atatttttat
    66901 gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga ggatctatcg
    66961 tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg tcaatcgtta
    67021 cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca tacggcacaa
    67081 attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg gcatcaacat
    67141 gtaaatagaa atagatccct tcatctttta acctttctct taattggacg attttatcta
    67201 cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct agaattggga
    67261 ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc attcgataat
    67321 cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata tctaacgcct
    67381 tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt tggatatttt
    67441 ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta aagctttcgg
    67501 tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac atttttttca
    67561 ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa cgggcgtacc
    67621 ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat ccgtctgtca
    67681 tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa gttgccatag
    67741 aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa gctaataaag
    67801 caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa tgccatggga
    67861 tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata tggtcgcggg
    67921 tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata tctgaaggaa
    67981 tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac attttataaa
    68041 ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata tctttcattt
    68101 ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta tctgtaacac
    68161 ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat ccacgtagat
    68221 ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca aaaaagcgaa
    68281 caatttttca ataaatcagt cttc
//
LOCUS       DAJSVP010000006        68072 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSVP010000006 DAJSVP010000000
VERSION     DAJSVP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.006"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(144..2330)
                     /locus_tag="ON449_000360"
     CDS             complement(144..2330)
                     /locus_tag="ON449_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3932982.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2548..3231
                     /locus_tag="ON449_000361"
     CDS             2548..3231
                     /locus_tag="ON449_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3932983.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3312..4031)
                     /locus_tag="ON449_000362"
     CDS             complement(3312..4031)
                     /locus_tag="ON449_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3932984.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4343..4549)
                     /locus_tag="ON449_000363"
     CDS             complement(4343..4549)
                     /locus_tag="ON449_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3932985.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4794..5396
                     /locus_tag="ON449_000364"
     CDS             4794..5396
                     /locus_tag="ON449_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3932986.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5537..6817
                     /locus_tag="ON449_000365"
     CDS             5537..6817
                     /locus_tag="ON449_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3932987.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6830..7558
                     /locus_tag="ON449_000366"
     CDS             6830..7558
                     /locus_tag="ON449_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3932988.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7627..9150)
                     /locus_tag="ON449_000367"
     CDS             complement(7627..9150)
                     /locus_tag="ON449_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3932989.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9402..10583
                     /locus_tag="ON449_000368"
     CDS             9402..10583
                     /locus_tag="ON449_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3932990.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10643..12271)
                     /gene="treC"
                     /locus_tag="ON449_000369"
     CDS             complement(10643..12271)
                     /gene="treC"
                     /locus_tag="ON449_000369"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3932991.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12668..15379)
                     /locus_tag="ON449_000370"
     CDS             complement(12668..15379)
                     /locus_tag="ON449_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3932992.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15389..16090)
                     /locus_tag="ON449_000371"
     CDS             complement(15389..16090)
                     /locus_tag="ON449_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3932993.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16276..16875
                     /locus_tag="ON449_000372"
     CDS             16276..16875
                     /locus_tag="ON449_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3932994.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16956..17969
                     /locus_tag="ON449_000373"
     CDS             16956..17969
                     /locus_tag="ON449_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3932995.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18077..18994
                     /gene="rbsK"
                     /locus_tag="ON449_000374"
     CDS             18077..18994
                     /gene="rbsK"
                     /locus_tag="ON449_000374"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3932996.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19022..19417
                     /gene="rbsD"
                     /locus_tag="ON449_000375"
     CDS             19022..19417
                     /gene="rbsD"
                     /locus_tag="ON449_000375"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3932997.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19417..20790
                     /locus_tag="ON449_000376"
     CDS             19417..20790
                     /locus_tag="ON449_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3932998.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21147..23264
                     /locus_tag="ON449_000377"
     CDS             21147..23264
                     /locus_tag="ON449_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3932999.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23316..24356)
                     /locus_tag="ON449_000378"
     CDS             complement(23316..24356)
                     /locus_tag="ON449_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3933000.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24633..26435)
                     /locus_tag="ON449_000379"
     CDS             complement(24633..26435)
                     /locus_tag="ON449_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933001.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26449..27201)
                     /locus_tag="ON449_000380"
     CDS             complement(26449..27201)
                     /locus_tag="ON449_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933002.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27210..27572)
                     /locus_tag="ON449_000381"
     CDS             complement(27210..27572)
                     /locus_tag="ON449_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3933003.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27748..28620)
                     /locus_tag="ON449_000382"
     CDS             complement(27748..28620)
                     /locus_tag="ON449_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3933004.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28671..29159)
                     /locus_tag="ON449_000383"
     CDS             complement(28671..29159)
                     /locus_tag="ON449_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3933005.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29297..30088)
                     /locus_tag="ON449_000384"
     CDS             complement(29297..30088)
                     /locus_tag="ON449_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3933006.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30143..30751)
                     /locus_tag="ON449_000385"
     CDS             complement(30143..30751)
                     /locus_tag="ON449_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3933007.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30744..31061)
                     /locus_tag="ON449_000386"
     CDS             complement(30744..31061)
                     /locus_tag="ON449_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3933008.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31217..31945)
                     /locus_tag="ON449_000387"
     CDS             complement(31217..31945)
                     /locus_tag="ON449_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3933009.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32104..32985
                     /locus_tag="ON449_000388"
     CDS             32104..32985
                     /locus_tag="ON449_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3933010.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33222..34265)
                     /locus_tag="ON449_000389"
     CDS             complement(33222..34265)
                     /locus_tag="ON449_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3933011.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34268..34936)
                     /locus_tag="ON449_000390"
     CDS             complement(34268..34936)
                     /locus_tag="ON449_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3933012.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35078..37414
                     /locus_tag="ON449_000391"
     CDS             35078..37414
                     /locus_tag="ON449_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3933013.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37529..37840)
                     /locus_tag="ON449_000392"
     CDS             complement(37529..37840)
                     /locus_tag="ON449_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933014.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38021..39496
                     /locus_tag="ON449_000393"
     CDS             38021..39496
                     /locus_tag="ON449_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3933015.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39543..40127
                     /locus_tag="ON449_000394"
     CDS             39543..40127
                     /locus_tag="ON449_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3933016.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40129..40548
                     /locus_tag="ON449_000395"
     CDS             40129..40548
                     /locus_tag="ON449_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3933017.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40829..41113)
                     /locus_tag="ON449_000396"
     CDS             complement(40829..41113)
                     /locus_tag="ON449_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3933018.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41465..42175)
                     /locus_tag="ON449_000397"
     CDS             complement(41465..42175)
                     /locus_tag="ON449_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3933019.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42172..43644)
                     /locus_tag="ON449_000398"
     CDS             complement(42172..43644)
                     /locus_tag="ON449_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3933020.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43782..44255
                     /locus_tag="ON449_000399"
     CDS             43782..44255
                     /locus_tag="ON449_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3933021.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44408..44959)
                     /locus_tag="ON449_000400"
     CDS             complement(44408..44959)
                     /locus_tag="ON449_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3933022.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45064..45594
                     /locus_tag="ON449_000401"
     CDS             45064..45594
                     /locus_tag="ON449_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3933023.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46077..46520)
                     /locus_tag="ON449_000402"
     CDS             complement(46077..46520)
                     /locus_tag="ON449_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933024.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46536..47696)
                     /locus_tag="ON449_000403"
     CDS             complement(46536..47696)
                     /locus_tag="ON449_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3933025.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48203..49903
                     /locus_tag="ON449_000404"
     CDS             48203..49903
                     /locus_tag="ON449_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933026.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50005..50838)
                     /locus_tag="ON449_000405"
     CDS             complement(50005..50838)
                     /locus_tag="ON449_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3933027.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51142..53307)
                     /locus_tag="ON449_000406"
     CDS             complement(51142..53307)
                     /locus_tag="ON449_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3933028.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53567..55861
                     /locus_tag="ON449_000407"
     CDS             53567..55861
                     /locus_tag="ON449_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3933029.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55854..56519
                     /gene="pgmB"
                     /locus_tag="ON449_000408"
     CDS             55854..56519
                     /gene="pgmB"
                     /locus_tag="ON449_000408"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3933030.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56512..57519
                     /locus_tag="ON449_000409"
     CDS             56512..57519
                     /locus_tag="ON449_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3933031.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57541..58560
                     /locus_tag="ON449_000410"
     CDS             57541..58560
                     /locus_tag="ON449_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933032.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58841..60208)
                     /gene="nhaC"
                     /locus_tag="ON449_000411"
     CDS             complement(58841..60208)
                     /gene="nhaC"
                     /locus_tag="ON449_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3933033.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60317..61738)
                     /locus_tag="ON449_000412"
     CDS             complement(60317..61738)
                     /locus_tag="ON449_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933034.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61981..63858)
                     /gene="tdc"
                     /locus_tag="ON449_000413"
     CDS             complement(61981..63858)
                     /gene="tdc"
                     /locus_tag="ON449_000413"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3933035.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64149..65405)
                     /locus_tag="ON449_000414"
     CDS             complement(64149..65405)
                     /locus_tag="ON449_000414"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3933036.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65517..65722)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            65985..67286
                     /locus_tag="ON449_000415"
     CDS             65985..67286
                     /locus_tag="ON449_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3933037.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67355..67954)
                     /locus_tag="ON449_000416"
     CDS             complement(67355..67954)
                     /locus_tag="ON449_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3933038.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20410 a        13801 c        12113 g        21748 t
ORIGIN      
        1 cacctccctt ttccgaataa actgtgttcc agaaatagct tcttcgaaaa taagctgaaa
       61 tttcacaaaa atttgagaag caattttcgt acattccttc ttctttcttg aaactgacca
      121 cttctgtcac accctctctt ctatcacttt ccttttttca cacgactccc ggtttctttg
      181 ataaatctcg atactttcaa ttttcttgaa tttttcattt gaggtacaca tagatacaga
      241 cgatttcttg ctctagtcat cgcaacataa aatagtcttc gttcttcttc tatcgtcgtc
      301 actggttctg cttttatttc attaaagtcg attactttct tttctgtcgc tccgttataa
      361 ggcaaacttc cttcgttaca accaatcaaa aagacatttc ggtaactaag acctttactt
      421 ttgtgaatgc tctggataca tagtgcatca agttttcctt gtctgtaagc aatcaagcgg
      481 gtttttaatt ctttagctgt cgcttgggtt tcttgcgata aggcttggag tcttctgaaa
      541 gtagcatgct gctttacttc ttctcgtatc cattcttgat cactctggtc gatttcctta
      601 gctatacgtg gcagatgaag atagctttga acaagcgaat agatttcttg atgctggtct
      661 gttttacgca acgcttcctc aaactctcgg atttctgcag atttcgattt taaattgggg
      721 cttatcgaca aaatataatc tgcaacggct atattttgat atttaggaga aaagtattga
      781 tcgcattcac tttttttcag gtagcagtct gccatcagct gccggagcaa ttgctggtag
      841 gcctgatttt tcttttgcag attcagtgct gtttgccaat gaacaatttt ttgatagaaa
      901 tgttggaaag acttgatacc aaagatacct tcatatggac tatctgcttc cagtacaaaa
      961 ggaatattcg ttttcagcag agattggaca agctgctgcg tttgtgaagc tgatcggtat
     1021 aatatgattg attccttata tttcccattt ttttgaataa atgtctgaat ttgtttgatt
     1081 agccactgac tttctgcttt agtatcagaa aacaaatagg cttccataac acctgcttct
     1141 ttttgtgctg gaatcatcgc ctttggtatc cgatcatttt tattatagct gatcaatcta
     1201 tttgctcctt ggacaatagc tgcccgagaa cgataattag ttaataagaa aagagttttg
     1261 gtaggatatt ctttttcaaa gttcttgatg tatgctggct ctgatcctct gaatccatag
     1321 atactctgat catcatctcc aacaacaatc aacttgatcc tgttttcctt tgtcatccgt
     1381 ttcacgattt cccattgaag tggattagta tcttgaaatt catcaatgaa tagataatca
     1441 tatttttggc cgatataatg ctggatctcc tcattctcca aagcgctttt cagattttgg
     1501 agtacatcat tgaactctag caattgctgg cttctttttt gttcttggag ttccgtgagc
     1561 aatccataca gtcgttctga tatcggctgt cgtgcattct tatcaatgga atcatatttt
     1621 tctttaacta actgaaccat ttcttctact gaaaggttca tattctgcca atagtctaac
     1681 cgagaagcaa tcgttttttc tccatattga tcaaatatat aaagattata tttacgtatc
     1741 agctgttgca aaacagcatt cactttttgt gcagtatcct ttgaaaagtc tccttgtatt
     1801 ccttgcattg ctacttcagg aaattttcga caaatttcat tgtatatcct aaaaaagata
     1861 ctatggaatg ttccgatatg gacagcggtt tttctctggc tgaaattatc tactcgtttt
     1921 tttagttcat ttgcagcgtt ccttgtaaaa gtgatcatta gtatgcgttc ttcgtctact
     1981 tgtttttgcg tcatcagata aacagctttt gcacagattg tttgcgtttt gccgctccca
     2041 gctcctgcta tgattcgaag atgtttcttc atatcatatg tgatagctgc tttttgttct
     2101 tgattgaagc gattatagga acgtttctcc atcattttaa agaaagagct gtttccttca
     2161 ctttctttta cttgtacttc ttcctcaata agtccgggtg cttcgccata agccgcttca
     2221 taggtagcaa tgatttcttc ttgtagccga aagacttgtt gaagcttttt atacttgttc
     2281 tcccagcgtt tcaattcttt ttgtaatcgg agaatttctt tttcattcaa ctgattgatt
     2341 tccttcccat ctttcttctt tgaatttgtt tatttcaagt tggttattta aagtttcgtt
     2401 gtctattttt tctttatact aacagacctt tctaaaaatg aaaatgggaa gcgatatgat
     2461 tttagttttc tgaaaatttt tccttttttt caatcgttta tgctaagata aggaagacaa
     2521 aaatattcat aaaaggtgat gaaaaaaatg cagacttcct ttcacgaggg gcgtacaata
     2581 ggacttttgc tgacatttat cggaggagcc atggacagtt atacttatat ccaatatggt
     2641 gcttttgcct ctgctcaaac tggcaatatc attctatcga tcatccaagc atttgacgga
     2701 gaatggctaa gtgttggtaa aaaagtattg tctacccttt tctttttctt agggattctt
     2761 ctaacaaagt atctaattga ttattttaga aaaaaagaaa aacatttctg gcgcttgttt
     2821 gttctatatt atgaagcagt gatttttttc tttgttagtt tgacacccgt ccatgagcat
     2881 cccgcattag taacgatttt gattgctttt actgcatcga tccagtgggt agcttttgat
     2941 aaaatcaacg ggcgtgccta cactaattta tttacaactg gaaatttaaa aggggtagca
     3001 acgaacttat atgattattg tatttctaaa gaaaaagcag attttgatcg atttttccac
     3061 tatctaagtg tcgttcttgc ctttatcgca ggagctattg tccttgtttt ctgtcatcat
     3121 ctgtttggcg cccattcgat tttgatcgtt tccattttat ttttgatcat ggccctcact
     3181 cagacatttc atttatggca attttatcat acgaatcggt tcactatttg atctcaatga
     3241 atcaaaaaaa ctaagcaaga tgctgctcac agagaaatat ctgcgagtga gcaatttgct
     3301 tagtttttat attatttagt tgtaaagatt ccctttgctt tcgggttatt tgtagtgtag
     3361 cggatcgact gggcatctgc ctcttctgtc attttctttg cccgcatctt gatttcttgt
     3421 acgatttgtt ctccacatgc cggatcggct acgaaatcat atttgtcccc atcgatcact
     3481 attttgggac atatatcaaa ctgttcatac cactcttcgt atcgtcgatt gagttcttta
     3541 tagtaggaat agagttcagg atcttgttct agctgttcat attcccggcc tctttttttg
     3601 attctcgcta acatagtttc aaaggataca cgaatataaa ccagtagatc tggtttcttt
     3661 tgtggcgaaa tttcttctaa ctccttcagc atcgtttcta aaagaccttc atactgcttt
     3721 acttctactt cagaaacacg ccctaaatct gcattcaaat gaaacagcat actatcttcg
     3781 tagatggagc ggtctagtac gttgtcatca tggctgaaag catctttgat tgccaaaaag
     3841 cgtttgttca aaaagaaaat ctgtaataaa aaagtatact tttctggatt agaataaaat
     3901 agcggcaata cttcattatc ttccacgttc tcataaaaag gttttgtttc taaatcttgg
     3961 gcaagcattt cagttagtgt gcttttccct gcaccgatcg ttcctgcaac aatgatcaca
     4021 attatcggca tccttcctgt aataaaatct gttcttcatt ttactacttc atttatagaa
     4081 ataaaagcct tgactttatc tgctccttaa aagctacttc tctaaaagta gccaaaagta
     4141 actcctattc atgaaaataa aggctgtgac aaaagtcttg tcgcagccct tttttcaaat
     4201 aaacagtatt ctcctgccaa cacctcggtc acaaggcaat attcataaaa catgaaaagc
     4261 tgttttttaa cattattcct tgtaactcag agctaaacgg gagactcaca acctctttat
     4321 gctctcaaca ttttatctgt ttttattctt ctattgtact tgatttgcgt acaactaact
     4381 gccagtgcag attatgagcg gtatcattgt cttctactgc gcatttcatg acacgtatca
     4441 tcacactatt ttcatagatt ttttctattt ttccttttat tggtgattca aataaatcag
     4501 atttaaatgc atatgtttcg ccgacttcaa acaattgttc attttccata agaatccctc
     4561 catcaccttt tataaggtca gtaatttttt aacataaatc actcgaaaaa gcacaaactt
     4621 gtcataaaaa gtacttttcg tatcaaaact gtcacttttt ttggtaaggt tcttggaaaa
     4681 cgaagatttt tttaggagat catgacggtt ttgatgtcaa aaccactttt tatgaaacaa
     4741 aagacgtttt ttattttttt tagcataaaa tttgtggtgg aggtacgtat gagatgaaag
     4801 ataaagttgt gaacaaatta ttagaagcct tattcagcgg agacaaatcg gaagaagata
     4861 tggtgtatgt ccaagtaaaa tttgccttag aagttttatt gaataatctt ggaaaactag
     4921 cggtcgttct agtcttttct ctggtgacag gctcatgggc ggaaacagga atcacctttc
     4981 tttcttatat ctgcatccgc agatatgctt atggattgca ttccgactct gaattcgttt
     5041 gtctgctgtg gacactactt tatttatggg gcgttccctt ggtgatgaaa catctgcaat
     5101 tgactatctc cttcccaatg atggtctttt tgttaatcag ctgctttttg ttactgcttc
     5161 gctatggatc aagaggtaca gcaatcaatc ccattgaacc cgagaaacgt cccccattat
     5221 taaagaaggc gatcagcatg tttctaattt tttctctcat tactttattt ttctcagctt
     5281 cttacttttc tacctatttg ctacttggga ttgttttaga aatagctacg ttgcttccta
     5341 ttacaaacta cttgatgaat tttggaggaa tctttcatga aaaaaacaat cattaaacaa
     5401 ttaactaact tatctgactt ttttgtatct ggctgctgct ttggtatgag ttctgaattc
     5461 aaaatccctg aaaaattaaa aaaataatac gctaattgtg ctataatttg gtaaaaatca
     5521 atgggagctg tactttatgt ttttatggtc catagcgttg caatatttga tttacacaat
     5581 ttgtatctta ttgattgacc atcacatccg atacaaatat ctgtttgcgg gagtcattct
     5641 gatttcttgt attttgattg gatgggtcta ttcgatttta gggattttta ctgctctaat
     5701 tgctatagga atgctgatgc ttactttttt cttcttttcg acttcgcgtc aacaattggt
     5761 ttacgcgctt ccttttgggt tattgacgtc gcttttagga gatcacttaa caagcatcga
     5821 cgattttttt gttttgaaat cttcggttat tgaaccagga gattcattgc agtattttca
     5881 cattgtctta tccgcttttt tatctgcttt attagctttt tgtttaaaaa aatcgttaga
     5941 gacttggttt cacactgtgg accgccgtat gatcggtacg atcgggacat tgatattatt
     6001 tacatactat atcattattt tttatacccg tttttctggt gaaacacctg atgtcttagc
     6061 tttgaatacg agttttttca ttctatatct gtgtgttggc ctgatcatct tggtttatta
     6121 ttggaaaatc tctaataaga aattagctga tcagttatta gagcaacaag tcaggatgca
     6181 acaggattat atcaaagatc tagaaaaaaa ctatcaagac ttgagagaat tcaaacatga
     6241 ttaccaaaat ctgctttttt ctcttcacag ctatatttca gaaggcgact tagaaggatt
     6301 acggtcttat tatgatcaaa cgatattacc tacacgccag atgatgaacc tttttccggc
     6361 caatctaagt atgttggata atatgaaagt tcctgagatc cgtagtttac tgtcattaaa
     6421 actcatgatg gcacaggaaa aagggttgat cgtccagctt cattttcctg agccaatcga
     6481 actggatacg aaacatacgg taaaccttgt acgtatgctt ggtattatct tggacaatgc
     6541 gattgaagga gctagtgctg caaagaagaa aaaaatcgaa ttgaccatac tcaagaagaa
     6601 tcattccgtg atgattcgtg tgatcaatac aacaacaaat acattaccgt taaaccagtt
     6661 gaaacaaaaa gggttctcaa caaaaacaaa tccaaaaaac gaaggccttg gcttgtttat
     6721 cttggatgaa ttgacaaaga ccaatcctta tcttttcctt gaaacatcaa ttgaaaacca
     6781 gcgtttttct caaacgattt atatacaaat aaaataaagg agtaatgata tgattcccat
     6841 ttatatttgt gaagacgatc caaaacaatt agaagtttta tcaacggtga tcaaaaatag
     6901 aatcatgatt gaaaacttag acagttatgt ccatatttct acctctgatc cggaagaact
     6961 tctcaatgca gccaagatca gaacttcttc ttttggtatt tactttttag atatcgaact
     7021 agaagacagt gaaatggatg gtgtagcgat cggtaaattg atccgtgaat tagatccttt
     7081 aggaaaaata atctatgtga cttcccatac ggaaatgtcg atgaaaatct taaaaagcaa
     7141 catcgaaccg actgattata tcgtcaaaga agatctttat gatttaaaag aaaatgtcga
     7201 gcatatcctg agcaaaattt tcgaagattt tcaaatgtcc caaatccaaa aagatatctt
     7261 caaaatagag tttaatgatg agatcaaatt tttaccgatc aaagacatcc aatatttttc
     7321 tacttcgcta ggagcgccac ataaactgga agtccatttg actcatgccc aattacaatt
     7381 ctatgagaag atcaaagaaa tcgagaaaat gcatcggaat tttatccgtt gtcataaatc
     7441 ctatgttatc aatacacaaa atgtgcggtc gattaataaa aaaaccagag aagtcacgtt
     7501 ggcaaacggc gagaccatcc cagcctctat ccgtggatta aagaaattga tttcataaaa
     7561 agaaccgaat agtttggacc gactaaatga ccagccgatc caaacccttc ggttcttttt
     7621 tgcctatcag tgcaaaagca tttcctgtgc aatttcacgt ccatccattg tatctggata
     7681 aaatgttggc caattctcca tttctttcag caatacttct tgactttcat ttcctgcata
     7741 taaatgaaaa tgttcggagt cagccggtgc aatcgtgtga tcactgaatt gaatatatct
     7801 aaaagcgcca ctttccttat cttctgcttc gaatagatac cttacgccac ggtttcctgc
     7861 ctcgtaggtc aagatttctt tccctacata acgataattt gaacgtttcg ttgttccatc
     7921 cttaaacgta aatgccatct ctttctcact gatcttgatt gaagcaatat ctgtttgata
     7981 gcccttcgta taatattctt tatattcttg tgctgtctta tcctgattca atttttcttt
     8041 atattcaaaa acaggatcaa aagtcccatc ttctacatac gtatctactg tttgccaagt
     8101 tcctgcccaa tctgaaagag agcgatcttg tacatcctcg tcttcaaaat aaccgttgta
     8161 gacgctcttt tcttttttgg gggtcaaagt atcttctagc gggttttctg tctctgtcgt
     8221 ttttttcaac gcttcgaggt ttgcttccat gatagaaaga tagttttctc cattttctat
     8281 ttgttcattc gtcaatcctt ccaaaggatt caatacttct aaagatacac ctgcttcgtt
     8341 tgctaacgtt ctggcaatac tatctttggc gttttcttca aaataaatat agttgattcc
     8401 atgttcttta acaaagtgtt tcagttcagc aattctcgcc gcagacggtt cttcactaga
     8461 agaaagacca gcaatcgata cttgattcaa tccatattct aacgctagat agttgaatgc
     8521 agcatgttgc gtgacaaaag atctttgttt tgcttgctta aaggtttctt gataaagctg
     8581 atggagtgct tgaagtttct cggtataggc tgctgcattt tttgtccata cttcctgttt
     8641 ttctgggtaa gcttcaatca gctgatcacg tatgtttgct acctgcttga ttgctaaact
     8701 tggagctagc catacatgtg ggtctagctc atgtgtatgg ccttcttccc cgtgttcatg
     8761 gtcatgatca tgtccttctt cacttcccgg aagtaaaagc ataccttctg tcgcttttat
     8821 cgtatggaca ttgccttctt gtaatgtttt ttggattgcg gggacccatg tttccatgtt
     8881 ttcatcatta taaacaaaga tatctgtatc ttggatcttt gccatgtctt tagcagaagg
     8941 ttcataatcg tgggaatcag tccctgctgg aatcaataac tctacttctc cttcatcccc
     9001 gacgattgct tttgtaaaat catacatggg ataaaaggtc gtcatcacgc tcaatttttc
     9061 tttactgtcc tcggctttcc cacttgcccc gcaagcagtt aacatacctg ctaaaaccag
     9121 catggccaat atacctatca atcgtttcat ttatgacact cctcttctca tcttctttca
     9181 tttgaatgtt ccgattcgct tttcgcaaac cgtaatagtt acgttttagc aacaggattt
     9241 aatttatcat atggttaaaa gaactgtcaa gcgttttctt attttaattg atgagaaagt
     9301 ttatatgaat gattgacgat atttttgcaa tcaacctcta tcattttcct tttatacccg
     9361 cttttgtcta taatgaagtt atagataaag gaggaaaaag agtgaaaaaa gaaacagata
     9421 caacgaatcg tttgatccgc tttttaggag gaaaaacctc ttactatgtt ttaggaatag
     9481 ttatcttact tgcgctgatc atttattttt tccatcagat ctcgtttata ttcaagcctg
     9541 tcggaatcat tatcgctact atcttgcccc cattattgtt tgcactgatc ttgtattatc
     9601 ttttggaacc agtcatcaaa tgtttgagca agcacctttc gaggaacttg tcagtcatta
     9661 tcgtttatgt gattatttta tttttattgg ggcttggaag catatggctt attccttttc
     9721 ttgaagaaca ggcaaagaca ttgatcagta gtttgccaga tttgtttcaa gattttcaag
     9781 agagtctccg acaatttttg gagaaaacac cttttgccga ttcatttaat caattttttg
     9841 cttctattga cgacgtgacg tctgatatag ctggatttat tggaaattat tgggaaagtg
     9901 gcgcacaacg gatcagcaac attttttcaa ctgttacagc tatttttatt actttattta
     9961 ctggtccaat tgtcgctttt ttccttttga gagatcctca aaaattttat cagtcagttt
    10021 tagcgattgt cccaccagca tttcgtacag attttaaaaa tttgacaaag attgctaatc
    10081 aacaattggg ttcatttcta aaggggcaaa tcattgcttc atttatcctt ggagcagtct
    10141 actgggtttg tttcttactg atcggattag aatttgctag tgttttagct atttctgctg
    10201 ggttgttatg tatcattcca tatattgggc cgtttatcgc cttctttcca ggtttgatca
    10261 ttgcattcca agactctact tttatggctg tcaaatttgt cattgtctgg tttgccgtac
    10321 aacttctcca cggtgacttg gtgattcctc gagtaatggg tgatcgttta cagatccatc
    10381 cgattacgat cttaatcgtt ttacttgtca tgggtgatct aatgggaatt gtgggcgtta
    10441 tttttggtat tcctatctat acgttagtca aattactcgt tacttttatt ttccgtaaat
    10501 tcaaacaacg ctacaataaa ttttatggcg ataaaggaga gtatgaaaac tcttcatttt
    10561 ctgaagaaga ttattttaaa taaaacacaa aacgtcgatc ttacgagaat agctcgtagg
    10621 aacgacgttt tgtgtttttg ttttaatttt tcagcaaagc aattgcctgg tatggtttta
    10681 gctccagact ttgatgaaga acagtatcgg aataattatc gataagaata ttggcttctg
    10741 tgaatccttc tgggatggcg atccttgtat ctttatcata gaaatttgtt aatacaagca
    10801 gctgctgtcc ttcaaattgg cgaagaaagc cataaacttc cggatgattt gcttcataag
    10861 catgataatc tccttgtgca acgattggat atgtttttct cagccgaatc aatttcttgt
    10921 aataggacaa aatagaatct tcagaagata gttctttttc tacattaatc tcttctagct
    10981 tgcctccaac ttttaaccaa ggtttccctg tagtaaatcc tgcttgttct gtgtctgacc
    11041 attgcatggg tgttcgagag ttatcgcgag attttgcttt gatccgacga aacgcttctt
    11101 tttctgtgaa tccttgttca aggagtaact tataagcatt caaactttct acgtcaatat
    11161 agtcggaaat cgatgaatag tctgggtcga tcatgccgat ttcttctccc atataaatat
    11221 agggcgtccc acggttcaaa tggatcatgg ttgctagcat ctctgctcct tgttttcgat
    11281 actgatcgat agctacaaat cgattgagcg cacgtggctg atcatgatta ttaaaaaata
    11341 gagcattcca gccgttaccc tcactcatct tttctcccca tgtatgaaat agtcgcttta
    11401 attctgcaaa atcaaatgga ggagtagacc atttattccc attttcataa tcgactttca
    11461 aatgatggaa attaaagacc attgataatt cttctctact aggttctgta tacaagatac
    11521 aattttcgat cgtcgtagag ctcatttcac cgactgtgat gatagtggaa tccgtcccaa
    11581 acgttgcttg gttcaattct tttagatacg tatgggtgat tggtttgtcc gtatattcag
    11641 gtttgccatc attttcttta ttatttttca attgttcgtc tttaccgata acattgataa
    11701 catcgaaacg gaatcctttt accccttttt ccatccaaaa acgaacaact ttgaatagtt
    11761 cctctcttac tgcttggttc cgccagttca agtcagcttg tgtccgatca tataagtgca
    11821 aataatattt ccccgtatct ccaaatggcg accaagcact tccaccaaat tttgataccc
    11881 aatccgttgg ctcttctctt aaaatgaaga agtcctgata atgctggtcc cctgctagtg
    11941 cttttttgaa ccattcatgt tctgtcgaaa cgtgattcag caccatatcg agcatgattt
    12001 ctatccggta tttctttgct tcggctatta gctgttcgaa ttcctccatc gtaccaaaga
    12061 taggatcgat ggctgtgtaa tcagagatat cataaccgtt gtctttttga gggcttggat
    12121 aaatagggtt tagccaaatc atgtcgatgc ctagatcatt cagatacggg agtttttctt
    12181 tgattccttt taaatctcca attccatcac cattactgtc tttgtaagat ttgggataga
    12241 tttggtaaat gactttgtct ttaaatgtca tggatattta cctccattca actgatttat
    12301 tacctaccac aacttatata tacaagttat catttattat tataactgaa ccgaaagcgt
    12361 ttgtaaaatt tatctttata cattctaaga ggtctgttgc ccaataaaaa acaagagatc
    12421 agacatgtat ttgtctatcc tcttgtttaa aatagcaaaa tattcttgtt tcttctataa
    12481 atggtgttca aaagctttta tctacggcaa tcagtcttct caaatgtaaa acatgaggag
    12541 ctaattttat ttgagtattc tttttgctcg atgcaggacg gcttttttac aacctctgat
    12601 tcatggtgtt caattagcag ctgaccctcg ttattagctc aaaacgctga tttcacaacc
    12661 tctgttttta ttcattcgat ttcaacgcat caatcatatc gatatgcttt aatttgaaat
    12721 gggtaacaat catcacaata accgtaaata cgatagttaa tcctgctgat aagacataag
    12781 cccaccatgt gatcactaat ggaaagatga cattctccat agacgcttgt tgcaagatga
    12841 agtcagtcaa tatatatcct acaccaaatc ctccgatgat tcctaaaaga gtaaagatga
    12901 tattttctcg aacaatgtac atcgtgactt ctttatcaaa aaatccaagg acttttattg
    12961 tagataattc gcgtacacgc tcggaaatat tgatattagt tagattatac aagacgataa
    13021 aagccagcag tcctgataat acgacaaaga ttaagacaac cgagtctaag ttcgccattg
    13081 attcttcttg cgtttcgatt tgtttggaca tgaaagacgt atttgtcaca gcgtctgtgg
    13141 caagcagttt ctcggaaagc atttcttctt cttttgttgt catcttatct gtacgcagca
    13201 aaaatgcatt atccatcatc gattgatcag taactgaagc ataatattca ggggacatat
    13261 aaataaagct acctagatag tttttcacga tttcttttac ttcgactttc cattcttttt
    13321 gatcttcatc ataaagcgaa atatgatctc ctgtagataa gtcaaatagt tctgctactt
    13381 tttgcgtaat agcgattcct gttttaggca aggagcgtgg gttctcttca agatcagtta
    13441 aatggataaa acgatccaaa tcagctgttt tacttggtac gatcactgtc agactttgcg
    13501 aagcatgtcc tttttttctc aattctacgg tttgagtatg aatagctagg atctcattta
    13561 ctttttcttg cttctgcagc atatccgctg ctttttgacg actatcttcc gtggatgaat
    13621 ccaatgtaac gattgcttga tagtcaatga tcggtccaaa ttgtttggct gaaacagaac
    13681 ttagagaatc tttcaatccc acgcctgcaa ccatcagccc ggcacatcca gcaattccga
    13741 taatcgccat aagcattctt gacttatatc ggaagagatt tcggtaactg attttttgat
    13801 taaagctaag gcgtgaccac agcggagtga tgtattcaag aaaaatgcgt ttccctggct
    13861 tcggtgcttt cggttgaagc aatgcagcag gccgttctct taaatctttg aataaaacga
    13921 tcattgcagc gccaaatgcg gcaatgaaga atgcggcagc tgcttgtaaa atcggcggcc
    13981 agtcataaaa aataagcgca ctcccaatat cataccgctc gttagacagc tcaaaaatga
    14041 tccttggcaa aagattcgtt cctaatatcg ttcctaaaac aatacctatc cctgaggcta
    14101 gacttgcata gattgcatat ttttttgcga tttcaacttt cgtataacct aacgccttta
    14161 atgtcccgat ttccctgcgg ttttcttcta ccatacgtgt cattgtcgta aaggtgatca
    14221 atgcggcaat aaagaaaaag aaaacaggaa aaacattggc aatcgcagcg atacgatcag
    14281 acagatcacc aaattcttga aaaccgacat tttctgaacg ttcattgtat aggtatgatg
    14341 gtttagttaa attttcaatt ttttctgcat ttcgattcaa ttcttcctct gcagaggcca
    14401 gttcacgttc tttctcaact aattgccttt cttgttcagc aagttgagct tcttgttgag
    14461 ataatgtttg aacagcgatt gttcgttgcg cttcaggaag ctgctggata gttgctgact
    14521 gttgcgctaa ctgtttttta gcatcttcta accgtgttct tgcttgcgtt gtttgtgctt
    14581 tgccatccgc tattttttgc tgtgccggtt ctaattcttc gttggccgat tttttcagct
    14641 cttctaagcg ttcttcgggt cgatcttgta aatacttttc tgccttttct tgattttgct
    14701 ccattttatc tttgtaagct ttgctgtacg tattcaaatt ctttacgttt gtaaaatcaa
    14761 gatataagac agaataaata tctgttttaa aaacggattc aggtaaatag acaaaataat
    14821 cgacgctacc atttccgacg ttcgcgtatc ctctctccaa attgttgatg aacaatggag
    14881 aacggacaaa gcccgtgatg gtatactctt tttctttcaa ttcatctgaa tcaatctgat
    14941 aaatatcacc gattttatac ccttcatctt ccgctttttc atccaataca agctggtttg
    15001 gtttttccgg gagttttcca tcagttaatt gtagcttgtt ctgcttgtta tttttgttat
    15061 aagaaaatat ttgaatcact tcgtttttgc cagtattgga ataatagatc tgttttcctg
    15121 tttctacttt tgcatctggg atcttttctg cttgagcaat atctttttgt gtcaagccac
    15181 cagtagaaac gatttgcaaa tcagaaagtc ctgctgtacc aacatagtga tccatcgttt
    15241 tttgcatgac aggtccagca gttttgacac ctacaaataa aagtgttcct agcatgacaa
    15301 taaggatgat tgcaatgaat cgtcctttag attgccgaat ttcgcgaaaa cctgctttaa
    15361 gatatgttct tgttttcata aaatccccct accatacaat ctcatcaatg gatgaaggat
    15421 gatcatttaa ttcaacagac cttactttag catcattaat gtgaatgact ctatcagcga
    15481 tgggcgctaa agcagaatta tgtgtaatga tcaaaacggt attgccgtgt tgtctgctag
    15541 cgttttgcaa taatttcaat acttgcttgc ccgtttcaaa atccagcgcg cctgtcggtt
    15601 catcacataa aagaagtttg ggatttttag ctagtgcacg agcaattgaa accctctgct
    15661 gttcgcctcc tgataattgg gacggaaagt tattcaatcg atgagctagg cccacttgcc
    15721 tcaatacctc tacaggatca agcgcatcag gagaaacttc cgttgccaat tccacatttt
    15781 cttttgctgt tagattagga acgagattgt agaattggaa aacgaaccct acatctgttc
    15841 gtcggtaagc agtaagctgt ttatctgaaa attgcgcaat atctgtgtca tcaataatga
    15901 tctgaccttc atcaggtgta tccatcccac ctaaaatatt aagaatcgtt gactttcctg
    15961 ctccacttgg accaagtata atcactaatt cgcctttttc aattgaaaaa gaaatatcgt
    16021 catttgctgt aatggtcgtt tctcccattt tgtatcgttt gtattcattt tttacttcga
    16081 catacttcac tctgattcct ccattttcac gatatttctc gctatatcta tgcatcgaat
    16141 gcgttttctc acacttattt attttaccac tgaatttctc tagactaaag aaaggaatcc
    16201 tttaatttag ttgacaaata aacgatttat gatttactta cataaaaaac attgttattt
    16261 gaaagaagga acatcatgaa tgatttaaag gaaaatatga tttttgattt atcaaaaact
    16321 gcttttgtcg cgatcgacat gcaaaaagag atcgtaaata acggtacact ctctccacac
    16381 actgcagcat ctattctgac aaacaatgat ttgttagtaa gaacattgaa acgtaccgct
    16441 gccttgaaag ttttagtgaa cgtagacatc tccactttcc cttatttgag tcaacaaact
    16501 gatatgggtg gaatgactgc ttctgtacct ccagaattca ctgatttact tttgaaggat
    16561 tctttgaaag atacagataa tatgttgact atcacaaaat acaatccaag tgcttttttt
    16621 ggtacttctc ttgatcttca acttcgccgg cgtggaatcg aaactatcat tttaagcggt
    16681 gtagcgacaa caaacggtgt atatgctact gcattagatg cctttcaaca cgggtaccat
    16741 atcgtgcttg ctgaagatgc ttgcagtgat cgtgacaaag aatcccatca attgttcata
    16801 aaaaagatat ttccgaaaac tgcacgagta cgctcaacga aacaaataat cgaagctatc
    16861 caacaaagca aataagtact ggcacacgtg attttcattt acttcttttt gatttcatgg
    16921 gaaaataacc acaggattta gataggagta tgaacatggt gaaaaaaaag atcacaatca
    16981 aagaagtcgc taaactttcc ggcgtttcaa tcacaactgt ctccttgata ttaaatggaa
    17041 atacggagaa attcagtccg aaaactgttc agaaagtttt agctgccaaa gaagaattag
    17101 gctatcagcc tgactatttt gctagacaaa tgattaccaa ggaaacgaaa acaattggtg
    17161 tactcgttcc tgatatcacg aatccattct tcagtacact gatgcgagga attgaggata
    17221 tcttgtataa gcagaatttc gtcacgatat tatgtaatgc tgattctgat catcaaaaag
    17281 aaatcgaata tctagccgag ctcacacgtc gaggtgtgga tggattcatt atcgcgactt
    17341 ctgctgtctc tacagatgca atcaacgaaa atttgaaaaa acaaggccgg cccttcattg
    17401 ttttggatca gaaaaaatct gaaggattta gtgatgccgt acgaacagac gattttcgcg
    17461 gtggttacct agcgggcatg cacttgctta gtctaggaca tcaaacgatc gcccttgtct
    17521 atcctgaaaa tccaccggaa aatgtccacg ctcggattga ggggtttaaa tctgctcttg
    17581 atgtttacca aatccctcac gaccaactga tcctcttacc tacacagttc tcaaaacaag
    17641 gtggctacca gatcacagca gaacttcttg attctgctgc aactggtgtg tttgctttaa
    17701 atgatgaatt agcttttgga ttatatcgcg gattagagga agctggaaaa agcatccctg
    17761 aagactacag cattattggc tatgataata tagatatgtg cgagtatatc aagccgaaac
    17821 tgacaacgat cgcacaacca atcttcgaat tgggacaaac atctgccaag ttattgttag
    17881 atcggatcca attcccagaa aaagaatggg aagaaaaaag attacctgta cgattagaaa
    17941 aaagattttc aactgcaccc ttgaaatagc tttcaaggtg tgctatattt ttatcgaact
    18001 attaaaacgt tttactaaaa cgttttaaaa aataattttc ccatcaaaat aaatgagata
    18061 gttggaggtt tgtaaaatga acacggttac agtaattgga agtattaata tggacactac
    18121 ccttcgttta accaacatgc ccaagccagg cgaaacgatg catgcgcatg agatttttca
    18181 cgctggcgga ggtaaaggtg ccaaccaagc agtggcagcc aaacgttcag gcgcacggac
    18241 atcttttatt ggaggcgttg gggctgacag cgaaggacaa cagttattag atctactgac
    18301 aaaagaaaac atcgatacat ctgggatagc tgaaatccaa ggcgccacaa ccggacaagc
    18361 aatgattatg gtagacgcag caggagaaaa tagtatcttg atccatgcag gggcaaataa
    18421 tgctttccat gaacaagaag tcctaaaaaa caagcaactg attacaaaca gtgactttat
    18481 tattgctcag tttgaaagta gcttagatgc gacgatccta gctttttcta ttgcaaaaga
    18541 cgcaggaaaa acaacgatcc tgaatcctgc accagctcgt gaaacgattc ctactgaact
    18601 acttgaaaag acagatatca ttataccaaa tgaaacagaa acagaaatca ttacaggaat
    18661 cagagtgaca gatcataaca gtttggtcgc agcagccgaa aaactccatg aattaggtat
    18721 tggaacagta atcattacac taggcagtgc cggcgctttc tatcatacag aaaaagaaca
    18781 cggtatcgtc cctgctttta aagtggatgc cgttgacaca acagccgcag gtgatacatt
    18841 tattggcgca ttaagcagca cacttcagcc agaccttagt aacttgaaag aagcgatcct
    18901 ttatggaaat ctagcctctt ctgttgctgt gcagtcttat ggtgcgcaac cttctatccc
    18961 ttaccgcgaa gctttagaac aatcagcagt ctaaaattaa caatgagagg aagaaacaaa
    19021 catgaaaaaa ggaaaagtaa tcaattcaga tatttcacga gtaatcgcac aaatggggca
    19081 ctttgataaa ttaagtatcg gggatgcagg catgcctgtc ccctttggta ctgaaaaaat
    19141 agatttggct gtctcaaacg gtcttccaag ttttttatct gttttagaaa acgttttaga
    19201 agaacttgag gtacaatcga ttgaattggc ggaagaaata aagacaatga acccggaaat
    19261 attggaaaaa atcaaattat tgttaccaga tacaccgatt agctttatcc cacatgaaga
    19321 aatgaaaaaa gagttgaata actgtcatgc atttattcgt acaggcgaaa tgacacctta
    19381 cgccaatatt atattagtga gtggagtggt cttctaatgg aaacgacaat gaatcctgaa
    19441 cagatggtac tgtccgagca gattcatatg ctgattgaac agttgggagg aaaagacaat
    19501 attgattctg tccttccatg tgcgactcgg cttcgtatca aattgaaaaa acagccagta
    19561 gccaatgcag aaaaattaaa acagcaaatc ccttccatta agtatttcat tttactcgat
    19621 agatatgcag aaataatcat ggatcaggga ttggctcctg cttacaagga attgacgcgt
    19681 caaatcgatc attcagctcc gaaagaaaag ctaaatccga tcgaaaaact atttggtttg
    19741 atatcggaaa tctttacgcc gatcattccg ctgtttgccg gttctggtat attaaaaggt
    19801 ctggttgttt tagcgactac gattggctgg cttagtgaaa caagcggaac ttatcatatt
    19861 ttgtctgctg cgtcgaatgc cgtcttttat tttctgccaa tctttcttgc tttttctgct
    19921 gccaaaacgt ttaaagtgaa tggatatatt gcggctgtca ttgctgcagc actgattgaa
    19981 ccgaatctga cctctttact ttcagacagt ggcaaagcag ctactgattt tctggggatt
    20041 cctgtagtac tgatgcagta tacgtctact gtcatgccgg cgattttagg tattttcttt
    20101 tattcttttg ttgaaaaatt cctcaaaaaa tatattcgcg aaaatatgca attagtattt
    20161 gtacctttac tttcattagt tatcacggtt cctttgacgt taatggtatt cgggcctgcg
    20221 ggtgtttacc ttggcgaagg tttagctgcc gcaattactt ggatgatgga tattaacgga
    20281 ccgatcagtg gcgcaatcat cgctggtggt tggaatattt tggtaatatt tggtatccaa
    20341 tgggctgtaa accctgtcat gatcagtaat atctctgctt atgggttcga ccgaatcgtt
    20401 ccgttgacag gagcagccaa ttttggaatg gctggtgcag ctttaggtgt atttctgcga
    20461 agtaaacgtt caaaaacccg ttcgatctcc ggttctgctt ttgcttcgat tttacttgcg
    20521 ggagtgaccg aaccaactgt ttacggaatt gcgatcccat tgaaaaaacc atttgttgct
    20581 gcttgcattg gtgcagctgc tggtggagct gtaatgggat ttgcccaagt gaaagcgatc
    20641 gcttttgtat ttggtagtct gaccacattg cctgcattca tttccggtac ttttttctgg
    20701 tacttagcag gactggctgt tagtctagtc gtagcaatga tcactactct cgttagcggg
    20761 tttgatgaag acctaatgtc ttatgaatag ttaatgaaaa agactgaagt atgagatgtt
    20821 tatcacatat ttcagtcttt ttctttttag gatcattatt tttataaatt tttgtgatac
    20881 tttccaaact ttggtataat ttacatatgc ataaccattt ttatccccac ttgtttatcc
    20941 ccaaacaaag gtggtctatg caataaaatg aacgtcaaag aaaaacgaat gaatgctcgt
    21001 tgcttattta caaattgttt tatttagaat acacagacag atttatgaaa agaaaaagct
    21061 tttcgacacg attatctgtt ccaaaaaatg tctaatgaat gctgaataag acagaaaatc
    21121 aatgaaagga aaaggactag tttattatga aaaataaaaa aacgatacgt ttgctagctg
    21181 ctacttggct gattgctccg tctgttttgt cgaccaatac cgtatttgcc gtagataatc
    21241 aaatagctga tcaaacagtc acaacagata cgactacatc cagctcagct actagtgaaa
    21301 caacggattc ttttacagaa gacccaactg gaacaactaa ctcaacaaca ggatcgaccg
    21361 agccaactga aattgatccg gctgatccat cagaaaatga aaagccagcc gagacatatt
    21421 cccttacaat tgctccttct ttgattggta agatccaact gactgcttcg acagcggatt
    21481 cccctgaaca gcaacctttg gatattacgg aaagtggaac ggtagaaggt ttattagcgg
    21541 aaacgaaggt aacttacact attacgccag ctgaaggtga acaaattgct tcagtcgctg
    21601 ttaatggtgc caaagaagta aattatgatg caaagacctt tacagtaggt acgaacaatt
    21661 tatctattac tgcagaattt aaagctggac cggatacttc aacagagaaa ccaactgatc
    21721 ctagtgaaga agtccctagt acaccagatc cggaaacgcc aaacaaacca gatgtgggta
    21781 ataacaacaa taataatggt tcaaataatt caaacaataa taacaataat tcgaacagcg
    21841 ggaattcttc gaatcaaaca aacccatctg gatcgaataa caatggaaat actcagagag
    21901 aaccaaacca tccaaacaat ggttcatcga atggcggaac gaacaatgct tcatctcgtc
    21961 caaatacagc aaatcaaagc ggtatagaga atccatcttc tgcttcttct gattttgttg
    22021 taaaatcacc aattgatgct gtattgccta caaatacaac gaatgtccaa caagcaatcg
    22081 ttcgagaagc attcaagcat cttggaaaac catatgtatg gggagcaaaa ggtccaaata
    22141 cttttgactg ttctggtttg acttattacg tatatatgaa agcaaccggt cattatatcg
    22201 gtggctggac aggcgaacag caatatgcag gtacgcagat tccagtaagt caggcccaac
    22261 ctggcgatct cgtcttctgg ggaccttctt ccggtgttac tcaccatgta ggtatctata
    22321 ttgggaacgg acaatttatc catgcgcctc aaccaggaga taaagttcga gtaacttcta
    22381 tttcagattt cacgcctgat tttgctgtcc gagtaaactt agcaggttta cctgctgctt
    22441 caggttcgct gacaggcggc tcatctattt tagatggatt agatggctcg ttccatttca
    22501 gtcaaaacca aacaactgat cagttcttga aaaagattgc agatgatgca caggaaatcg
    22561 ggcaaaaaga aggaatctac gcatcagtca tgatggctca agctatcctt gaaagtggat
    22621 ctggaaacag cttattatca agcgaaccaa atcataacct ttttggtatc aaaggatcat
    22681 ataaaggaag cagcgtgacc ttcaatacac ttgaacaaga tagttccgga caaagctacc
    22741 aaatccgcgc acaattccgt aaatatcctt cttacaagga aagtttggaa gactatgccg
    22801 acttgatcaa aaatggactg actggcaatc ctgatttcta caaaccaact tggaagtcgg
    22861 aaacaaaaga ctacaaagaa gcaaccaaat atttggaagg acgttatgct acagatcgcc
    22921 aatattcaca aaaattgaac gcgatcatcg aagcgtacga cctgacaaaa tatgatgaac
    22981 cgaaaaaaga agcagcaaca actgaagaag caaacgctga aatcctcaat atgtctaagc
    23041 gattcgatgt gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt
    23101 cttccatctt ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga
    23161 taccagaaac aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa
    23221 gtttagacaa atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg
    23281 gatgtgacac tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat
    23341 tccgttacaa actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt
    23401 cgctccgctc ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta
    23461 ttcccgcgca tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa
    23521 gcatctgttt ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt
    23581 agtaaaccag attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat
    23641 tggcgaaaat cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct
    23701 gtgccactta ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat
    23761 aatcggctat tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa
    23821 ttgaaataga catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc
    23881 gtcactcgcc atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca
    23941 ggaaatccgt tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca
    24001 ccattgatga ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa
    24061 caatgaccag tgtccgggtc tgtttttact tcgaacgttt catcatctaa tttgaatgtg
    24121 ccattcgtaa tccgtccagc catacgtccg attgaagcac cgatataagg gtctttttct
    24181 agatattctt ttgcttcatc aaaacctaag atcaactctc gtttttcatt atcgattatt
    24241 gtttgaaaat gaacaacccg tgccccaaga tcactgacag acaaagatgt cccattttga
    24301 ttcgtgatcg taatcaagtg atagttttcg ccaaaatttt gttcattgat ttccatgttc
    24361 cttgcaccct cttttctttc atttgcactg attccagtat acatggaaac cgttttctta
    24421 acagcttatt ccgtgagtaa cgagaaaaaa tttactatat tctaataaaa gaccgtgaaa
    24481 aaaaggttgt gacaaaaata ttgtcacaac ctctttttcc gaacaaacgg tgttcaaaag
    24541 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gaggagctat ttttgcttgg
    24601 ctgttcttat tacttgatgc aggacagctt tttcacaatc tctttttcaa tacgattcca
    24661 gtatataacc atgtcgtcag tacatagtag atgaaataca atacgaagaa gatcgtaaac
    24721 ggaacccaaa tatctttgta tggattttgc atgaatagac caaacatttg taatccgaat
    24781 aagacatgga ctacacctaa gattcctggt gctaagaata acacgcctac ttctctacgg
    24841 atcgattgtt taagcaaccc gcgtcgtgtt cctatctttt caagcatttc ataacgagca
    24901 atgtcactct tgcttcctga caagatcttg aacatcaaac agctagcaag catggtcaag
    24961 aaagctaaac ctaggaagaa tcccataaat tcaaaaccag agaatagacc attatacact
    25021 tcatacattt catatttctg gttgctgtac tctacttttt gcaaagatgg attgttcatt
    25081 ttattttgag caacaagtgt ttcgattggt ttcaggtttg cttggaagtc ttttacctga
    25141 actagctgta aagtggattg tggcaagttc agttgatcaa attctgattg actgactacc
    25201 ttgatttctt tttcttgctg ttccggcaat tcatagcttc gaagttcctc ttgtacagtc
    25261 agttcattca tttttttagc aggaactttt tctttcgtga tcttcggcgg tgttccatct
    25321 tcatgtttta tcatgatcaa tggttgagcc gcaaattcac ttgcattata gtaaatcgtt
    25381 cccgcatctt ctttttgagt ataagaagca gaaagtgttg gtgaaagttc tttgattttt
    25441 tgctgatctt ctgcccgagg attattcatc actagatcat atccagaatt gttttctgtc
    25501 aagtctgcaa tttcattttt aaaacctaat cctaccgtca aagcgcccag tgccaaggca
    25561 aacagcatag cgaccattga gagcatttgt gtatattcac gaatacgaaa gtttaactgt
    25621 gacaatgtga aattgttcaa ttttttcaat gaaatctgtt ctgatttctt tagtaatgat
    25681 aaaaagaaaa tgatcacaga atggaaaatg aaataagttc ctaatacgat cgtaactaag
    25741 gctaccccta taccaaagac ttgataggtc cccacgtgtt tcatcatata gtaaccgcag
    25801 cctagaaaag caattcccag tactacttca aggaaaaaca agaatttatg ctgcttgatt
    25861 cttgttggtg tctgatccgc acgaatcaat gctaaaatcg gttttttgac aatcgaaaaa
    25921 gcattgacaa cagcagctaa taaaaacaaa acagcaaaaa acaaaacagt gatcaacaac
    25981 ccgtgaatac taaaaggtgt aaaatgcgaa atattgagat ctaactgatc cactaataat
    26041 cggtggacga tacttgtcaa tcctacaccg acagctgaac ccactaaagt agctgcgatc
    26101 cctactaaga atgtttcaga aaaaatcatc tgtgcgattt ttctgccttt tgctcccagc
    26161 atcataaaca ttgcatagtc tttttgccgc atcgtcatca aaaaggaatt tgcatataaa
    26221 atataaacga atgtgatgat ccctagtaaa acagaaccga atcggaaaat gataactgta
    26281 gccgcaacga tcgtattact tttcaaaaaa gcttcgttgc ttgccattga ttcaaacatg
    26341 taaaaaatag cagatgccat aactaatcct gagaataaca ccaagtagtc tcttaaacgg
    26401 ctcttgatcc cagtaagtga taatttccaa agcatagtca atctccccct actgttccaa
    26461 gtttccaaga atagatagta tttctcgata gaaggaatca cggctttgat ctccacgttt
    26521 cacttcttga tggataccgc catccttgat gaataaaata cgttggcaat aacttgcaga
    26581 aaacggatca tgcgtcacaa gaagaatcga tacatgatct tttgtattca attcgtccat
    26641 cgtatctaaa agatcccggg cacttttgga atccagcgca cctgtaggct catcccctaa
    26701 tagaatcgtt ggttgtgtga tcaatgcacg tgcggcggcg acccgttgtt tttgtccacc
    26761 ggaaatctca gaaggatagc tttccaaaat atgagagatg gataaacgtt ctgcaatttt
    26821 ttggacacgt tgcttgattt cttttggtct gacgccttgt aaagaaagag gaacagcgat
    26881 attttcttgt gctgtcaagc tttctaataa attgaaatct tggaaaataa agccaatctc
    26941 attcgcacga aaatctgcta gttgatttcc ttttaatgtg gtcacatctt tttgattgat
    27001 tcggatatgc ccatctgttg gtttatctaa tgtggataaa atattcaaaa gtgtcgactt
    27061 accagaacca gaagcgccca tgatcccaat aaattcgcct ttttctacat cgaagctgat
    27121 gccgcttaat gcttgcgtct ttttctcatt gttttttccg tatacttttg ttacattctg
    27181 tacttcgact attttgttca tttctttccc taccctttaa ttagtttttc cagtgctgtt
    27241 ttcggcacgt cttttttttc gacttcttca tacgaagtga cacctttatt tttgttccag
    27301 gtgattttta aatatgctcc tttacgaagc ggtcgagtca attgtccctt gaagtccagt
    27361 tctttttcct taccctcttt cgtatagcca ggcaggatat atctatagtt gactgtttct
    27421 tgtccagagt ctgattttga aactgttttt gtaccatctg tcgtaatttg tacatagtat
    27481 tcttctccac ccataactac gtaatctgcg attttcaccc ctgcaagtac cacaacgatg
    27541 aaaacaagta gaccgatcag aagttttttc actttttccc cttctctctt actttatata
    27601 ctgattatag aaaaaagcga acaattctgc cttcgaatca actatcattc ttctttcaat
    27661 tttgtcattt tctttaaaac aactaaaaaa agagttcaag acaataatcc gtcctaaact
    27721 ccttcttcct atcttacaaa aagtatgtca cttacggtct ttcatgtcgc ctgtattgat
    27781 aacaggttgc gttcctttgt ctgtaatgat tgagacaagt aaattattga tcaattggac
    27841 tttccgatca tcggtgaaat tgatttcttg tccatcttcg atttgctcta aggccatttg
    27901 agtgattgtg acagctcctt caacaatggt ttgtcgagca gacaaaatgg cttttgcttg
    27961 ttgacgttgg agcatggcac tagcgatttc tgttgcatag gctaaatggt tcaagcgtgt
    28021 ttcgattact tcgacacctg ctactgctaa acgctcttgc agctcttttt gcaactcgtc
    28081 agacactgcc gtcgtgtttc ctcgaagcgt caaatcattc tcattgaatg tatcatacgg
    28141 gtattggcta gcgatatgcc gaatggctgt ctcactttgg atttctacaa aatcttgata
    28201 atacgctaca tcgaataatg cctttgctgt atcaacgact ttaaagacta ctactgctga
    28261 gatttcgatg gggtttccat ccaaatcatt gacttttaaa acagaactat tgaaattccg
    28321 aactttcaac gaaacagtca ttttttgtgc caaaggtatc gtaaggaaaa agccattttc
    28381 tcgaatcgta cctaaatatt gtccaaaaaa caaaattact tttgcttgat taggactgac
    28441 tacagttgcc gaacttaaca acaataacgt gatcaaccaa agaaaaatac ttaaaaaaag
    28501 tgcccaaact ttcgcttgcc acatacctaa atagaagaaa aacaatccaa ctaaagctaa
    28561 tacgaccaat ccgacaatcc ctgcataacc attgatataa aatgctcttt tttctctcat
    28621 gctacttcct gcgctccttt atccttcaat aaatatctaa tgttactttt ttaatttttt
    28681 tttcgaatac gcgccattac accttctagt tctaactctt tgtcttcagt gatgtaccaa
    28741 ccgcttgcct gtgtccctcc ggctgcctgc ggtaaataat ccaattttgt tcgaacccag
    28801 tgcttttctt ctgtaaaata gtcttctacc aagacctcta gatccatccc tgctgaaaga
    28861 ggcaaatctt cccgaacaga tttaaagata aatcgttcct tcttcccatc atacttcaat
    28921 attcctgtat cgataatcgg taaagaataa taatccagct ctttcttcac cgtcaataaa
    28981 tgtttgaaaa cttcatggta cacttgctct aattggcgac cttctgaatt gttcgggtaa
    29041 ttaaccatca ctttttcttt gtctggatca tagaaggttt ggcttagttg agtgatataa
    29101 gtatgtaatc tttccatttc tgtttttaac ttttctcttt gaccttcttc taccttcata
    29161 tactctttca ccctgttttc tttttagata ttttctttct tattttacta tagttatgtg
    29221 cgttaagcga tgaaaaacag caaagattgg aaggatcttt gctgtttttt cttaaaatat
    29281 ccagttgagt acaggatcaa tctgtttcat tgtcctcttg atccgtcgta atattgatcg
    29341 atgtgtcttt agcagtgatc tttagctttt tctgtccttg ttctaacaaa gtatttgtta
    29401 ttggctggta gtctttttca atataagatt caccagacgt ttggatagta tagccactgt
    29461 tttccatctc atgcaaagat aaattgccac cgtttaggat gacttttcct tctttgctga
    29521 tcaaactgtc ttctagatgg aagttgctgt tttcacctgt gacgatcaag ttatccacat
    29581 tggcctcatc taatcgaagt gagcctaatt catctttcgc agttattgtt cctcctgtaa
    29641 attcattgaa cgatacccag ccatcatgtg tcttagtatt cacttttttg attgatactt
    29701 cgttgatatc aattgcacta ttattccctt cgattgtcaa ttcagataat tcatgatctg
    29761 cagggacaac aatagtgatt tgtgcacttg tgtcttctaa actaatgatt gcacctgttg
    29821 attttttgac tgcattcaca gtcaacacgc catttttctc ctttatttct ggcttttctg
    29881 tcttatcata agtcgttttt atctcaaaat ccaatccgcg tatgaccgaa actgtttgat
    29941 tttctgcatg gatgacgatt ttgtttgcat ccttgatttt ttttgtctca gtaaccatat
    30001 cgatcaaccg aggaccatta tcccaagtca gactcttttg agcgcctaat gtataagttg
    30061 cacctgctag gaggccacca gtaagcatca gaccaatacc ggtaatcaaa aaatgtttca
    30121 atctcaattt atgtgtcctc cttcaaacgc ccgctgaatg cagatctctt tttgtaataa
    30181 aacgtcgtcc catccattta acaaagttca tcatgacata aaaaagccct tttgttcctt
    30241 tcagcaacag tggcgaaata aatactgcgc ttcctgtggc gaacagaccg acaccgataa
    30301 agaataatcc actcagaatc gattgtccaa acacactgat tcctgagatg atcatgaata
    30361 ggccaacacc taagatactg atacataaaa catagatact aaaaatgagc gctagtaaag
    30421 caaccaatag tcccccaata accaatataa accccagtgc tagtggaatg aggacaggag
    30481 aagcaaataa accgataatg attaaccaaa tcaggcgaat ttgtcttttt ggatttttca
    30541 ctaactcgtt ttctgcttgt tcttcttctg aaaagtaact gatttttaat tttaaggcaa
    30601 actgctttgg tgtaccatat tcatcaatga tctgatctaa tgtttttcca gaatctaaaa
    30661 aatattcctc ataatactgg atcaaatcaa aacgatcgtc ttcaggaatt tcttgaagat
    30721 gatccacaac ttcattcaaa tattcattca tttgtgctct cctccaataa ccggtctaaa
    30781 gaatctcgaa aaacaatcca ttcatcaata atttcttcta aatgacgact tcccattggt
    30841 gtcaacgagt aatatcgacg gtttctgcct tcaaaaggtt catcataggt ctcaagatat
    30901 ccttcttttt tcaatcttct caaaaccgga tatagtgtag attcactgac agagagactt
    30961 tcttgtacga tttttgttaa ggcataccca tacatatctt ccttgcttaa tgctgctagc
    31021 acagtaccat caagaagaac agttggaatc tggatgtgca ttacgtcacc ttctattctc
    31081 aagtgtaata ttttatttca atctatatta tacaacatat aatatagttt caaaatcagt
    31141 ttgtcaaata caaaaaaact gactaaaaga tttgatcctt tttagtcagc aaaaaatggc
    31201 ttctctttga tttttattat aacagtggca taatccaatt tgtccacatc cagctcagtg
    31261 gcatgataac gatcattgca ggtatcatgt cagctactgg gaacattttc acgttgatca
    31321 ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc tttaaaatct aagatcatgt
    31381 ctggtgtagt caatggataa ataaattttg ccaataagaa caaaatataa aaaataatga
    31441 attggggaat tgctatcaca gaaacgacat accctaaatt acacgcaaaa atagctgctg
    31501 tgaaaaaatc aaggacagat ttagaaatca aaatcgtact gtctcctgtc atcccagaat
    31561 ctaaacttcc atagatcccc gtaccacttg cacaaaatag aacaatgacc gtcactaatg
    31621 tgctgataaa ttcatcgtgg gtcataccaa gcttttcact agggaagatt ttagaaatag
    31681 gtacttgcat cagcgcagcc cccttattta tcccattacc taaatgaaca atcaaaccaa
    31741 tcgctgttcc tatcactaaa gcaaaaataa cagccggcat atttttcatc ggcgcaatgg
    31801 aatagattcc catccccatc gaacacacac caaaaaccat cgtcagctgt gctttaaaat
    31861 gttcacttaa tttatttcct actaatccgc caattagtcc gccaaacaaa acggaaccta
    31921 cattgatcaa aactccagtt ggcatcaaaa aactcctctc tttttctacg aagcgaaaaa
    31981 aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga aagattgtaa cagacagaga
    32041 agattacacc aattcatttt ttgtgttaaa gtattcttta ttggaataag taggaggaat
    32101 atgatggata tccataaatt cgaagttttt cttgatttag ccgaaacaat gaatttcacc
    32161 aaaactgctg atcgacaatt caccacccaa ggaaatattt ctaaacagat tctttcgtta
    32221 gaaacagaat tagacgtcaa attattcgaa cgcgcccata gaaaaatcga attgaccgaa
    32281 gctggaagct tattgctccc atatgtaaaa aatgtcgtag aacaatacca tgctatgcaa
    32341 gaagtgcttg aaacctatac gaaagacaac aatctttcat taaatatctt aacgataccg
    32401 acaatgatca attataaagg tttttccaaa atcaccgagt ttttgaaacg ccatcctgaa
    32461 tttacagtcc agttgaaaga agtggaaagt gtcgaattaa cttacaatga cgccaatgaa
    32521 catagtgaca cgatccactt tgctagaagt ttccaggccc caccaagttc gattgagtgg
    32581 ttgcctactg aaacagacga ttttgtagca gttctaccta aaaatcatcc tttagctagt
    32641 aaaaagaaat tggatctaag cgaactgaaa aaggagaact ttttgctcct tggccccact
    32701 acgaacttgt atcaacctgt actagatctg tgtcgcgaag ctggatttga accaaaaatc
    32761 tcttataaag gagcaagaat cgacttgatc atcaatatga ttgcaaatga catggggatt
    32821 gctattgtta tggaaaaaac agtcaagaat ctattaaaag aaaatactgt gattctccct
    32881 atttctccaa caacagagag ttatcttgca tttactcgta aagtcggtga gcattctttg
    32941 gcttcagata cattttggga gtatttaaat caagaagaaa actaagccct acattttctg
    33001 tccatctatc cacccaaaaa aagagagcga cgcatgactt tcgctctctt tcttgcatca
    33061 tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt caataagaaa gcagcgattt
    33121 ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa aaaaacgact aaaaacccct
    33181 aaaaaccttc aatagaattt ttaggggttt tattcttttt cctattgttt acttaaccgt
    33241 atcattgctg tcgttcctac tccttcgata ctgctataat caatacgtcc atcatgcagt
    33301 tctagaattt ttttaaccag tgaaagtcct agcccattcc cagaaatttg acgacttgta
    33361 tctccttgat aaaatttttc aaacattcga tctctggtct cttcattcat tcctacacct
    33421 gaatcggtca cttccagtac aatctctgtg gctgtctcaa atagtttgat atgaatctgt
    33481 ccattgattt gattgtactt tatggcgtta tccatgatat tcagccacac ttggtacaaa
    33541 aactcttcat tgcctgtgta atttactgca gcaagttcga tatccaattc tagctgttct
    33601 ttctcccatt taggctgaaa aaaaacgatt acttctctga tttgttcatc taatcgatac
    33661 tctttctttt ccagttggat acgctggttt tccagtttgt tcaattttaa gatgttctct
    33721 gttaattgtg aaagctgcgt gatactttcg ataatccgat ataaaaatat ttggcgttct
    33781 tcgtcagaga gatttggggc ttgtaacaat tgaacatatc cttggattgt tgctagtggt
    33841 gttttgaatt catgggatac actggacaca aagtcattcc tcagtgtctc gatactattg
    33901 agttcttgga ccataacgtt gaaatcttta tacagttgct gtacttctgc tactttctgc
    33961 tgttcatcca ttcgaatcga aaaatctccc gttgctacca gactcatatt tgtccctagc
    34021 gcactgattg gatgcaggat acgccgccca acaaaaatcg atattcctgt acccacgacc
    34081 atactgaacc ccgcaaagac gaataacggg tataacgttg gttttccagc cgattcagca
    34141 ggcgagatag aatgtcgaga caataagaaa ataaaaccga gaaaacacag cagtgtcact
    34201 aagatcgaaa tgaagacaat actagcaaaa tagatccata attgtgaaaa tagctttttt
    34261 ttcatcttta aataaccgcc ttatagccta gtccccgtac tgtgatgatt tgaaaatctt
    34321 ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac atcgatcgtt cgttcttctg
    34381 tattcgtatc taatccccaa atatcatcca tcaactgttg gcgagtgaag atcttgttgg
    34441 gataagacag taatttatac agaagcagaa attctttttg aggcagaatc aacgaatgct
    34501 gatcttgtaa cactgtataa gcatctagct ctaattttgt ttgtccgatt atcaagctac
    34561 tgctgtaatt gatctttgaa cgtcgaagca atgcttccac acgtaggatc atctcattga
    34621 catcgatggg ttttgtcata taatcatctg ctcctaattt gaacccgcgt ttcttatcct
    34681 caaaatcggc ctttgccgta atcattagaa caggtgtgtc aaatctggtg ctacgtaatg
    34741 agtctagcaa gtcatagcca tccattttcg gcatcatgat atctgtaatg attaattcta
    34801 tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt caatgcggtg atgactgaaa
    34861 agcccgctcg ttccaacaca gaacggtaaa gcaactgtaa ttgcggatca tcttctacta
    34921 ctaaaatacg attcattgat ctccctctgt tttttgaaaa atttctagat tttttcttgt
    34981 agttcatctt cagttaacat ttatttcgta tactacgatc attgttctga tatacttatt
    35041 agtataccga atatttcaat acgggagaag ggtgattatg cttcaattaa aagatataaa
    35101 aaaatattat aaagtcggcg aaaccaccac caaagcattg gatggtgtgt ctgtcgcttt
    35161 cagacaaaaa gaattcgtag cgattttagg tcctagtggt tctggtaaaa cgactatgct
    35221 gaatgtgatt ggtggactag ataattatga ttccggtgat atggtaatca acggaaagtc
    35281 tactaaagac tttaaggaca gtgactggga tgcctaccga aacaactcta tcggtttcat
    35341 ttttcaaagt tacaacttga ttggtcatct aggcattatt gaaaacgttg aacttgggat
    35401 gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg gcagaagatg cattgcaccg
    35461 agtcggcttg acaaatcata tgcataaaaa gccaaatcag ttatctggtg gacaaatgca
    35521 gcgagtggcc atcgctcgag cactagccaa tgacccagat atccttttgt gcgatgaacc
    35581 aaccggagcg ttagatacag aaacaagtat ccagatcatg gaattgatcc aagagttatc
    35641 gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa ctggcccatc aatacgcaga
    35701 tcgaatcatt gaattttctg atggtaatat cttaacagat tcccatcctc acatcgaacg
    35761 accaaaagat gatcaattta acttacgcag aacaaagatg agtttttgga cagcgttaaa
    35821 gctttccttc aacaatatcc gaacaaaaaa aggacgtact ttcttgacct cctttgcttc
    35881 cagtattggg atcatcggaa tcgcgatcgt tctgtccctt tcttctggat tccaaaaaca
    35941 aatcgataac acgcaagctg aaacgatggc gaaatttccg attactattt ctaaggtaac
    36001 gactaaccag actcgtgacg atgccggatt gggtgcaagc aaagcggatt atccagacag
    36061 caaaacgatt actgcaaaag tcagcgaaga agataaagca cagcacacga ataagattga
    36121 tcaaacatac gtcgattacg taacagacat cgatcctaac ttaagtaata atatcggatt
    36181 tacccgtact acaggaatca atcttttacg tgatgtaaat ggcaaagtac agcctgtcag
    36241 cttctcgaat caaaatcctg atacggaatc tctttcttta tctagtacga tgtcagcaat
    36301 gacaggtgtg ggagtgtcaa gtttccctac acagcttgac actagcaaag aaaacttttt
    36361 aaaagacaat tattctttac ttgccggaag ttatccagcc tcagcaacag atgtcgtgct
    36421 gatcgtagac ggcaataata atactaatat caacgcattg aagaatcttg gatttgatgt
    36481 gaaagaagac gaaaaacttg attttgatga tatcgttggt acaacattca aattagtaaa
    36541 caacaatact tactatacaa aacttccaac tgggaatttt atccctaata cagattatga
    36601 tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg gggatcctac gcgtgaaatc
    36661 ttcttctaca atgaatctct tatctccagg aatcgcttat agcgatcaat taacgactca
    36721 aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct caaagagaca gtggtgtaaa
    36781 tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa acattgctta gttatttagg
    36841 tggagacagc ttgccttcaa gtatcatgat ttatccgaac aattttgaag acaaagaaaa
    36901 gatcttggat tatctagatg attacaataa aggcaaatca gatgaagata aaatcattta
    36961 tactgattta gctggaacga tgactgaact gactggcgga ttgatggatg ctatcactta
    37021 tgtccttatt gcttttgcag ggatctcttt agtaacaagt atgatcatga tcagtatcat
    37081 tacttataca tctgtgattg aacggacaaa agaaatcggt gtactaaaag ctttaggtgc
    37141 acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg tgtattttag gtatttcttc
    37201 tgggatatta ggtgtgttta ttgcatggct agctacgttc cctatcaata gcatcttata
    37261 caacatgacg gatctaaaaa atgttgctca actaaatcct gtccatgcga ttattctagt
    37321 gatcgtctct actatattga cgatgcttgg cggtcatctt cctgcacgaa tggcagcgaa
    37381 aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt gacaaccaca tttagatttg
    37441 agtcataaga gcgctgaaat agtagaaaac agcaagaaac gaggaagtaa tatgttcctt
    37501 gtttcgcgct gtttttttta attatttttt ataattctgg tatacttttc tttcctttgt
    37561 accacaactc ttctggcgta tacctgtctt ttccatttat gaatactgtt ccatctgctt
    37621 caatcgttac ttcagtccca atccacatat tacgagaaag tgcgagaaga ttttttcttt
    37681 ttcctcttcc tttttttcca aattgcaagg catcggttat ctcttctgtc caatgagatt
    37741 tcacgtattt tacttctccg ttttcaaatg taactttcaa aatattcgct atattttcca
    37801 cttttactgc tttaggaaat gactcctcca tttttttcac tttccttcct ccaacactaa
    37861 gtatgcgctt tcttttagtg ataagtatat catagtttat tctattatca aaaaaacgca
    37921 tccattccat tgtagaatac tttaccgaac aagttgaatt gttttcctct ttttcacctt
    37981 ttactataga taggtaaaga caactaagga ggaatctctc atgacagatc aaccgtatga
    38041 tttaagaaaa gtcatcgaag aactaaaaac acttccaggt caatatcacg aaacagatat
    38101 tgaaatcgat cctgaagcag aattatctgg tgtttaccgt tatatcggtg ctggcggtac
    38161 agtaaaacgt ccgactcaag aaggaccagc tatgactttc aacaatatca aaggtttccc
    38221 cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg aaattttgca
    38281 ccatgattat cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc caatcaaacc
    38341 tgtgaaagta gacaaagcag acgctccagc acaagaagtc gttcatctag ctaccgatgc
    38401 agacttcgat attcgcaaca ttttgcctgc acctacaaat actcctgaag atgctggtcc
    38461 atacatcaca atgggtgtag tcttaggatc agatccagaa aaaacaatga gcgatgtaac
    38521 gatccatcgt atggttctag aagataaaga tacgatcggt atgtacatca tgcctggcgg
    38581 acgccacatt ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa tgcctatcac
    38641 tatcaatatt ggtttagatc cagcaattgc cattggcacg actttcgaac cacctaccac
    38701 tccacttggc tacaatgaac tatggtgtgc aggagctttg cgtaacgaac ctattcaaat
    38761 cgttgatggt atagcagtcg atgaagtggg tattgctcgt gctgaattca tcattgaagc
    38821 agaaatcatg ccaaatgaaa caatgcaaga agatatcaac acgaactcag gaaaagcaat
    38881 gccagaattt cctggctata ctggggatgc taatccagct gtacaagtag tgaaagtcaa
    38941 agcagttact caccgaaaag acaatccaat catgcaaaca acgattggtc ccagtgaaga
    39001 acacgtatct atggccggta ttcctacaga agcaagtatt cttgccttgg tcgataaagc
    39061 tattccagga aaagtagtaa atgtctacaa tcctcctgca ggtggtggta aattgatgac
    39121 catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc aagcagctat
    39181 tcttgcttta tcagccttca aagaattgaa gacagtcatt ttagtcgatg aagatgttga
    39241 tattttcgac atgaacgatg tagtttggac tttcaacact cgtttccaag gagataaaga
    39301 tatcatcgtg atcccaggaa tgaggaacca tccattggac ccatcagaat taccagaata
    39361 taatcctggc tctattcgtt tccgtgggat aagtgcaaaa actatctttg acggtacagt
    39421 tcctttcgat atgaaagaac attttgtacg tgcaaaattc caagaagtag aagattggga
    39481 aaaatatttg aaataatgaa tgatctgcaa ctgttttgac atcataaaaa ggagtgcgga
    39541 aaatggaaca attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt ggcgcttcag
    39601 gcaccattta tgccatcaac ctagtgaaga aactaaaaga atatccgatg atagaaacac
    39661 acggaatcat cagtccatgg gcaaaacaga acttaaagct ggaatccgac ttatcactaa
    39721 ctgaattcga aagcctgttt gattaccatt attcaaacaa agatttaggt gctgctgtcg
    39781 ccagcggatc atttttgacc gatggaatga tcatcgtacc ggcaagtatg aaaacagtcg
    39841 caggtatctc tgttggttta ggcgacaacc tgattgcacg tgctgctgat gtaacaatga
    39901 aggagcaacg aaagttagtc attgttcctc gtgaaacgcc attaaatgcc attcacttgg
    39961 aaaacttgac taaattagca cgactaggtg tgcaaatcat accaccaatt cctgcttttt
    40021 ataatcagcc tcaaacgatc caagatcttg ttaatcacca tacaatgaaa cttttggatc
    40081 aatttgaaat tgtaaatgag cattcaaaac gttggcaggg tgattaatat ggaaaaagaa
    40141 acgatagttg aagtaaccaa actaggtttc acgatccaag ctgctgttaa agtaatcggt
    40201 aaagacttat tgatcacttt aacaggcgga gatacaccac atatcggaac agtcactaca
    40261 ttttctcgtg atacagagat gcaaactgta cgtttcccta gccacgatgg tcggttccat
    40321 aaagacgatg tcttagccga aaaaatagct gtgatcatca aaactgccgt tccgggaagt
    40381 tgtacgatca cttccggtat ccatgttgac catatctcca atgcacaaat caacgcctct
    40441 tttccgatgt cagaagagtt aggttacaag attttagatt ggttggaaaa aatagatttc
    40501 catatcaaag aaccagttta ttataaagat ggtgaaaaac cattatgaaa gaagaggttg
    40561 tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc ttctcatatt
    40621 ttgccgtttt tgagctaata ccgagggaca gctgattgaa cgccgtttat caagaggttg
    40681 tgataaagcc gcttcgtttc gagtagtaag aaaaattttg taaacatggg tttccacatt
    40741 tttgcataaa tttgacttac taccgagaaa ctggcttttg aacaccgttt atcaagaggt
    40801 tgtgacacgt cttttgtcac aacctctttt atttttgctt cactgtaact gctacaatat
    40861 caccgatttc ttttccgatg tttttacgaa tatcttttcg tagtccaagg atgtagcaga
    40921 ctgaaccatc tggatttttc acgcccatat tcacgatact gccttcatac ggcacattgt
    40981 caaaagttgc ttggacttta actcttccag caccgaattc ttttcttata tcatacggaa
    41041 aaataatata tgccccgccc ttgccgactt cggaagcata gatttttgat tcaaaagaat
    41101 aaatttccct cattttattt ctcctatttt ttacatctag tatagcatag caaaaaaaag
    41161 aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg tgttctcctg
    41221 ccaactcctc ggtcacaagt cacaatagtc aacaaacatg agaagctgtt ttttgaaatt
    41281 gctccttgtg actcagagtt aaacggcagg ctcacaacct cttccttaaa cggtgttcaa
    41341 aagcttgcat ctacggcact aagcccaatc agatgaaaaa tatggagtgc tatttttatc
    41401 tgattgttct tactgcttga tgcagagcag ctttttcaca acctcctaca tggtgttcaa
    41461 aatattattc gccttttgct tctttcacta attttgcgaa tgcttgttgt tggaattcag
    41521 cagctaaggc tttcgtacgt tgttcttgct ctgctttgct catttttgga ttttcagtca
    41581 ttgcttttac cataccgtct tgaacataag cttttgcttc actttccatc gtactttgct
    41641 gtttcttcac taattcagct aacttggctg cttggtccgt acttaattct acccatgtct
    41701 ctgggatttt gaatgtattc actcggctgt catattcgtg attgttatca gcaatattga
    41761 accagaattt atacatgtcg tttttgtatt cccaataagt agttttcgtc actttttcag
    41821 catcgccgtc tgtcttttct actttatttg tttcgttatc cgtaccagtt gcttttggtt
    41881 tttcttgttt ttcatttgtt cggtaaagat acactttttc ttttccattc cctaaagctt
    41941 tatacaaaag catatctgct ccttgggaat cagcactgga aacaattttt tctgtttttg
    42001 tttccgttac tttttccatt ccaaagtgat tggacaaatt agcgacgatc aaacttagtg
    42061 aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt tttttagcga
    42121 acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa aactccaata atcatgcatt
    42181 atcccccgct ttcattagaa caggttcttt tttcttcaat aaaaaggcaa taataaagcc
    42241 aagaatccca aagatgatcg ctacgataaa cgcggcatgg taacctgaca gtgtggcatt
    42301 gattgcctga tctttataag ctaatggcgt attatccaat acagatttgc ttgggatatt
    42361 tgcttttgtc acattcgtca acacactgat caaaatagct gtaccaatcg agcttgcgat
    42421 ttgacggaat gtattattta ccgctgtccc atgactgatc aagttatttg gcaatgcatt
    42481 cataccagaa gtcgttaccg gcatcatgac catggagatc ccaaacatac ggatcgcata
    42541 aaggatcacg atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg gtgcagttgc
    42601 cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt tatcgaaaat
    42661 cgcacccgtc acaggcatca tagcccccat gatcaatgca cccggcaaca acatcaagcc
    42721 agaatggaat gctgattctc cacgaatatt ttgaatatac aatggtacga ccatttctgc
    42781 ccctaccatc gccatatttg ttacaccagc taaaacagct gcaatcgtga atgtagatga
    42841 tttgaataca cgaagttcca agaatggatg ttccaaatgt aattgacgcc atgagaacaa
    42901 ggcgatgaaa atcacaccaa caactaagaa accaattact ttcaagcttc cccagccgtc
    42961 atctcctaca ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg ataaaatagc
    43021 ggataaaata tcaatgctag gatttgacaa tggaataaca cttttcatta ggaagaatgc
    43081 taaaaccaag acaagaacaa cgattggcag taccatacca aacaagtcac gccaatggta
    43141 gttatcaatg atataaccag ataatgtagg gcctaaagca ggagcaagtc caataacgat
    43201 ccctgacatc cccattgctg atcctctttt ttctggcggg aaaatagata gcatgatatt
    43261 ttgcaataat ggcatcgata ctcccacacc gcaagcttgg atcaaacgac cgattagtaa
    43321 tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga tcgtcatggc
    43381 agaaagatat aaatttcttg aactgaattt attgatcagc catgctgtta ttgggatcat
    43441 gatcccgttg actagcaaga aaccagtcgt taaccattgc acatcagacg ctgaaatatc
    43501 gaaagctttc atcaatgtag gaaaagctgt cgtcaataag gtctgattca aaacggtaca
    43561 aaatgtacca attaataaga caacaactaa aagtgtccga ttatatggtt ttccgtaaat
    43621 atctacgggt tggttgttac tcatatttct ccttctttct gaattttttc tgaaactact
    43681 agaatacttt agtcctaaat tcgctatttg tcaacctact tgacacataa gttaatacgg
    43741 atataattaa gggaagcaag aggacaaaag gagtgacaaa aatggttggg gcttccctta
    43801 aacgtttgtt ggaaaatgat caattggttg ttggcatcag tgaattaagt gatatcgtag
    43861 gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg attcattcgt tcgatcgaag
    43921 ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt cataaaagta gaactgatca
    43981 aaaaatattt agatgacgga tttactttgt caaaagctgc agaaaaggca gaaatccgcc
    44041 aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat atcgaattga
    44101 tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa agaagagaat gctaagatgt
    44161 acatcattca tgacaacgag acagacgaat taacttacaa gctccttcct gtcgatgaaa
    44221 aagctgacta cgaacagttg ttaaaagaac tctaaacgcc actaatctcc taagtaccaa
    44281 gctttttaat aagtagatgc tacataaata cttgaaaaaa ggcctgtttg aacataagaa
    44341 tatccgaacg gtgatctgcg tttctttcat atagaaagga attctccatc actgttcgga
    44401 tattttttta tttttttcgg ttactttctt ctaatacatc tgttagccaa tttgcatatt
    44461 ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt tcatgttttt
    44521 ctttttcagt aggaaagatc gttttttttc gctctaatag atgaagtcgt ttttcttcat
    44581 gaaggtcacg ctgctgctga ataatttcta ctaatgccgg atcattcacg tctttgacga
    44641 aatacaattt cagaacaaat tcgtctcgat taaccggtag ttcattggtg ggtgtatgaa
    44701 tccactctgc caattgttcc ttcccttctt ctgttaattc ataaactttc ttttccaatt
    44761 tttcacctgt gatctctact tgatgctgaa tgattccttg ctcttccatt tttgcaagtt
    44821 caggataaat ctgactatgt tttgcttgcc aaaattctcc tatttcattt ttgaacgatt
    44881 gattcaattc atatcctgtc atcttcttct tacttaataa ccctaacaaa atatatcgaa
    44941 gtgtattttt tcttgccatc agttttccct ccttgacaaa tctttaaatc cattctatca
    45001 taaatgagta atcgaaaaca tgtagtttat tacatgtttt aatttataag gagatgctta
    45061 aacatgaaag aatttaaaga attggaagat tttattggaa ctcactttat ctatacgtat
    45121 gacaatggtt gggaatatga atggtatgcc aaaaatgacc atactgttga ttaccgtatc
    45181 catggcggaa tggtcaaagg ccgttgggta aaagaccaag aagctgatat cgttaaattg
    45241 acggacggcg tattcaaaat cacttggaca gagccaacag ggacagacgt ggcattggac
    45301 tttatgccaa atgaaaagaa acttcacgga atgatctttt tccctaaatg ggttgaagaa
    45361 catccagaaa tcactgttac tttccaaaac gaacatatcg cagaaatgga agcagctcgt
    45421 gaaaaatatg aaacttatcc aaaattactt gttcctgaat ttgctacgat cacttatatg
    45481 ggtgatgcag gtatagacaa tgaagatgtt attagcgaag aaccatacga tggaatgcca
    45541 gatgatatcc gtagcggaaa atacttcgat gaaaattata aacgagtaaa ataaggttgt
    45601 gaaagagagg ttgtgaaatc agcgttctga cctgagtatt agagaaagaa acggaaaaat
    45661 agttcctcat gttttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
    45721 ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa taagaacgct agttagaaaa
    45781 tagcttttca tattttctag gctagtgggc ttattaccga gaagcagccg tttgaacacc
    45841 gtttatcaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaatggaaa
    45901 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
    45961 cgtttatcaa agattgtaac aactgcatat tcacacagtt aaattagtga cttcaaaatg
    46021 aaataatgct cccagcaaaa aattaggctg ggagcattat ttcgttattc tgttcatcaa
    46081 aaatcagcgt tttgattcca tttcatcaca agttcctcct catttactgc ttcatctttt
    46141 tgctgtgttt gaattgtaaa accttgtgaa agatagaaat gaatagctga ttgatttttt
    46201 tgatagactg tcaaagtcag tgttttattg gtttctttta tttttttcat tagttgcgta
    46261 ccgattcttt gttgccggta ttcccttttc acaaagattc ctgcgataaa ctgatcaatc
    46321 accccagcaa aaccaataat ttcttgatct atttctgcta agtaaatcac tgcatctggt
    46381 aaagctttcc taacagattc tacttgattc tcccaataag ttgatggaat gaattcatga
    46441 gaatccaagt tagcatgcag ccaaatcgat agaacagatt ccagttcttt ggcagacaat
    46501 tttgttgttg tgcggatcat tttttctctc cttacttatt cattgatttt aaaataaaaa
    46561 ccattttttt gaagctcttt tacaagtttt cgcccctcaa aaaagtatat cttctcttgt
    46621 tttacttttc taataccaaa agaaacagca actttaggat catcagctga aacgattcga
    46681 taagttccac caggcaattg atccagcttg actttcccat gatggtcgct ttgtactgaa
    46741 aacggcacac catttctagt aagtgttttt ttcttatgtt tcaaaatgaa tgaactacca
    46801 tttaccggtt tccctgacga atcactaata ataaatgaaa aatcaagcac ttttttactt
    46861 aatctatact gatagattag tcgtccgata aacactaaca gtatgactac agctattatc
    46921 gttcctgcaa gaataaacca ttgccgcatg gttctttcct gatttccttt tccttcttct
    46981 ttttttaccg attcgttata aggaatccga tgtcctgtca ctagcaaacg atgcgtgttg
    47041 atcatatacg gcgtacatgt aagcaaggta actaagtctt ctccttctga aatcgttaga
    47101 acagacgtgt catctggcag taccacttgt atatttttaa cttgataagc caatttctcc
    47161 cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac gatctaattc ggtaaataac
    47221 cgtcgattag gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt tccaccaagc
    47281 gggaaagagg ttccttgcaa gacgcctgct ccattttcaa ggatcctacc attgagccga
    47341 tcataaagtg aaatatttaa ttggatctta ggtatattga tacttccgat cagccattcc
    47401 tctaattggc tggatgttgt actttcattt ctgttctttt cattgaaagg atcagtacca
    47461 ggattgaatc cttggtcagt taatcgttgg tttttctcct ccattttctt tcgctgcact
    47521 tcaatttcag ctgcggtccg tttttgtact tgttcgatac gtttttgatc aataaaacta
    47581 tttaaagaat ccacataaaa tggatataaa gcgatcagta accctacgac gatacatatt
    47641 aataaaaaca aacgaaacaa aagtcgacga gactgttttt gcttactctg cttcacatgt
    47701 gtcctccctt gcaagttttt ttgttctttc ttttattata tgggttcagg aaaaaaatag
    47761 aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agtacagccc
    47821 aaaaaaatag ctactcatat tttttgttgg gttgggctta ttaccgcaga tgcaagcttt
    47881 tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat gctttgagta ataagagcgc
    47941 tagtcagaaa atagcttctc atattttcta ggctagtgag cttattaccg agaagcagct
    48001 gattgaacac cgtttaccga gaggtcatga aaaagctact ttgtttcgag tattaagaaa
    48061 aattctgttt caaaacaagg agatgcaaca aaacttatgt cgcatcctct ctccttagaa
    48121 ctttcactta tgtttttctt gtaaaattca taaatatttt catgaatttt ctaatttctt
    48181 gcctacctct cgcataggat atatgatgaa tttatctaaa gtttgcaaag gagttcgaca
    48241 attgaaaaaa tatggcccat ttttagcaat ttttttcatt gctttggcat ttctcatgcc
    48301 tcatttattc acacataaac taattttggg tgctgatgcc ccattccatt acaatcggtt
    48361 ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat tattttcctt ccctttatgg
    48421 attccagcaa agtggcagga tcgtgaatgc cgtttacggc ccatttttcg cttatttaca
    48481 aggattgatc gtgttgcttg caggtagctg gtttcattat cagattcttt caaatttcat
    48541 tctttatctt atttcaggat tttctatgct gattttactg agatatgtca atgttcgaaa
    48601 ttgggtaagt gtaccagtag ccgttttatt catgagtacg tattctattc aatactggtc
    48661 tatcaaccaa ggattcacta gctgggggac ttgtttttat cctttatgta tgatccctat
    48721 tgctgatttc gtccttcata aaaagttccc tgttatcaaa gtcgctgtct cagtgggact
    48781 tatgacacag atccatttac tgtcttcgat catgttagct ttgatgtata tcccttttta
    48841 tctttattac ttttttaacc agactgaaaa aggaaaagca ctgcttcaac tgattaagtc
    48901 ggtcttgctt tattttgttc tgacagcaaa tatttgggca ggattagcta ttgtttacag
    48961 tgggaataag attcttcctc cctttgttaa cagagaaatg agtcaaaagg cgatagatct
    49021 ggatggcagt tattggctcc attaccctaa ggcctttcct atattgatat tatcaggttt
    49081 tttgcttttt attttctttc aacaaaagaa caccttattc caaaaaatcg tgttgtacac
    49141 aagtcttgtt ttcttttttc tttctactag cttgattcca tggacgacat tgatcgaaaa
    49201 aaatgtgcct tttattagtt tgatccagtt tccatttcga tttttcgtgc catttacgct
    49261 attgcttcta ctttaccttg ccctttcact gaaccagtgg tctgaacaaa agtggttccg
    49321 tcttttaagt atatttggca ttcttatttt ttcagcacag acgatccaga acttatacca
    49381 gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga catacttatc tattcgatac
    49441 gccggaagaa gcaaggaaaa cattcttcag caaagatatg tctaccagct tgttcgtttt
    49501 ccaaaaaacc acacctgact atctgccaat ctataaggag acaaaagaca ataaatacga
    49561 ccggtataac gaagaaatac tagaaaacga aacaactttt ataaaaacac atccaggcgg
    49621 tcttttgaca atcaagtgga aaaacactgg ggacaaaact gttcatatcc ctgttatcgt
    49681 ctacgatcgg actgtgctac agcaaaatgg aaaaacattg actgattatg aagtaacaga
    49741 tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc ttcattatca
    49801 aacgccaatc tatttctatt tcattctttc tcttactttg attggctggt ttaccttgct
    49861 atgtttattt atttatcata gatacaagct tttacgtgct taggcaaaag ggataaatac
    49921 atccgatcaa aaaactctcc aaacggtata atcgtttgga gagttttatt ttaatcagtt
    49981 tttttatgtt atatgttata ttttttattc gttcacatct aattcgattt ccaccccgta
    50041 gtgatcactg atgattggac tatcgatccc atcaaaaatc cgttgatatt ttttaacctg
    50101 cattccttca gggacgaaaa taaaatctat acgcaatttt tctgtatttt gttcccaacc
    50161 atctattttc ttttccacag tagctgcttt tcctttttca ttcgcgacaa catacgaatc
    50221 ttgaagaaga aggcaacttt ctcttacagt ataataacct tgagagtcta ctggattatt
    50281 gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt ccaagttttt
    50341 ccactcaaaa taaaagccac tttttttatc tgtccaccaa gaaaagtggc agctcgagac
    50401 aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag atcttgcgtg tctgataatc
    50461 ttctttcttt tgaaattggg agacggtaag gacctgactt tccaatgttt ttttcgtaag
    50521 cagtgcattt ccttcttcat atttatcata accgatgtgg ctcatttccc aactccaaaa
    50581 atactctttt ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat tatctttacg
    50641 gataggtact tgattcttta ccggacagaa cttcattagc ttcgcaggtt ctacttcttc
    50701 agattccagt aattggttga tttcttgtaa agcaatcact tcataatctt ctaacaggat
    50761 cttatctgct aattcttgca attttttctc tggttctggc tcaagccagc tatgcgtgtt
    50821 gagtgtcatg aatttcattg gttttctcct tgtcttcatt tcgaaacaaa agggttgtga
    50881 caaaattttt gtcacaacct ctccagcaaa tagtgttctc ctgctaactc ctcagtaata
    50941 agctcaatgg accaggagct attttttgtt tggctgtttt tatcacttgg tgcagggcag
    51001 ctttttcacg acctctccac taaacagtat tcaaactgct gcttctcggt aataagccca
    51061 accaaacgaa aaataggagg agctattttt gtttggctgt tcttatgact tgatgcagga
    51121 cagctttttc acaacctctc cttacatttt aatggagccg atcactttac cagctgtcac
    51181 tgtacctaat tgcttgatca ctacttcttc tactttatcg tttgtaaata cgatcatgat
    51241 cgttggatct ttttcttcgt tttggatcac atcaagatcc atctcagcta atttcgttcc
    51301 tgttcctact tcttggcctt ctttgacagc aatctcaaaa gctggctgat tcatttggac
    51361 tgtatcgatt cccatatgga tcaaaacttc gataccatct ttcgtttcca gcccaattgc
    51421 atgtttgcta gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga
    51481 tgttggaata acagcaaagc cgtcacccat cattttttgt gagaataccg gatcttttac
    51541 ttcagataaa ggaatcactt ctccagaagc tgcggaaact agttcttgtt ctatacctaa
    51601 gtattgctta ctacctgctt gtccatttcc tgtagaaacc atttctggct ctggaatctc
    51661 tgctcctgat tgcagcagat cttcgatatc tgatttcaag acatctgctt ttggtccata
    51721 aactgcttgg actccattgt ctttgacgat caatcccatc gcccctgctc gtttccaggc
    51781 ttcttcagtc cctacttttt ctcgatcatt tacacttacg cgtaaccgag tcatacaagc
    51841 atcgacatct ttgatatttt cttttcctcc cagcaaatga atgatctgga ccacttgctg
    51901 atctgctgtt cctgttccgc tgttagatac aacggtttct tcactgtcat tgtcgtaatt
    51961 tccgttacgt cccggtgtag caaagttgaa ttttttgatc aagaaatttg ctaagaaata
    52021 cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg ataagatctc cacctagacc
    52081 cgctttgata gctaacggtg tccgagtcag caattcgata ttcccaaatg aatggacccg
    52141 caatggcaag atatctgcca tcgcaaatgc agccccttgg atcactgaat aaatgacata
    52201 taatggcaca gcagcaaaca taaacatgaa ctccaatggt tctgttacac ctgttagaaa
    52261 gacagccaac gctgctgaaa aatacattga tttatatctt gctttcttat caggatcgac
    52321 attacgatac atcgcaaaag ccattcccat caagatacct gaagaaccaa tcatttgtcc
    52381 tactttgaaa cgagccggcg tccagttatg caacacaaac tcatattgcg acatatcccc
    52441 tgcaccttta agattcacta aatcagtggc ccaagccaac cataacggat cttgcccaaa
    52501 tacttgtgtc cccgcctgtg cgcctgataa aatctcatac gatccgccaa gctgtgtata
    52561 gttgatagga atcgtcagca tatgatgcaa tccaaatggc aatagtaaac gttctaatgt
    52621 tccaaataaa aacggtgcta gaatcggtgc gctttcttgt gattctgcaa tccataaacc
    52681 aaaattattg attccagcct gtacgtttgg ccacaccaca gcaagaatca atgccacgat
    52741 tgttgaccat aagataacaa caaacggaac aaagcgcttt ccattgaaaa atgacaaagc
    52801 atccggcaat tttctataat tatagtactt attataagcc attgccccga caaatccagc
    52861 gatgatccct acgaatactc ccatattcaa agctggtgct tccaatacac ttgtaaaaaa
    52921 tcctttaacc atgatcttcg taccgaacag agtgtgggta aatgcatctt cattcgcaag
    52981 catgtcactt gtgacaccaa agatagcccc agtgatccga ttgattaaaa taaatgagat
    53041 cccagctgca aatgctccgc ctgctctttc ttttgcccaa cttcctccta ttgctaaagc
    53101 aaataaaata tgaagattac caataattgc ccaaccaata ttttcaatta ctccgcccgt
    53161 tgtgacaagc ggcgtccaat caggattgat caagggaatt gatttcccta tactgatcat
    53221 caacccagct gccggcataa ctgctaccac aaccattaaa gcctttccaa atttttgcca
    53281 aaactcaaaa ctaaatatct ttttcattcc gtttagctcc tttacgcaat cggtttcgtt
    53341 aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt tattttttat ttttctgtct
    53401 ataccattct ataaaaccta taatacgtgt gttcccttct ataattttct cctaaaaatt
    53461 tattttttta agcttttttc tatgaaatat tttcttttgt ctattgactt gtgcaatcgg
    53521 ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa
    53581 acgactattt gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct
    53641 tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt
    53701 tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc
    53761 cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat
    53821 caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc
    53881 ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg
    53941 tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag
    54001 cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg
    54061 aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga
    54121 tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac
    54181 taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca
    54241 tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg
    54301 ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt
    54361 tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac
    54421 gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg
    54481 atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt
    54541 caatttgttc caactattct ctacttacta cggcgaagat gaacgattga atatcggccc
    54601 caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc
    54661 tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg
    54721 gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct
    54781 ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga
    54841 ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg
    54901 cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg
    54961 ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg
    55021 cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg
    55081 ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat
    55141 cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga
    55201 cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt
    55261 atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct
    55321 tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac
    55381 ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga
    55441 gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa
    55501 agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac
    55561 agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt
    55621 tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg
    55681 gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga
    55741 tgaacaagtt actttcgagt tattagaagg ttctcctcta gaaatttacg tctatgaaca
    55801 gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta
    55861 aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg
    55921 cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc
    55981 taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac
    56041 actctttttc aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag
    56101 agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt
    56161 ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc
    56221 tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa
    56281 caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag
    56341 cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg
    56401 gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc
    56461 ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat
    56521 ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac
    56581 gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca
    56641 gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta
    56701 acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct
    56761 tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg
    56821 aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt
    56881 gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc
    56941 acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa
    57001 accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac
    57061 atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa
    57121 ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca
    57181 acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat
    57241 catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg
    57301 actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat
    57361 ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa
    57421 atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa
    57481 cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc
    57541 atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct
    57601 cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt
    57661 atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc
    57721 agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt
    57781 tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca
    57841 atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa
    57901 aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat
    57961 ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa
    58021 atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc
    58081 cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc
    58141 gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt
    58201 gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa
    58261 gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa
    58321 atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc
    58381 tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt
    58441 gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg
    58501 gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa
    58561 atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga
    58621 agatactact tctaacacat tgtttatctg taaaaaagag cgtgggacaa aagtaaagaa
    58681 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
    58741 aataagctga aattcacaaa aatttaaaaa gcaattttct tgagtttctt cttatttctt
    58801 ggagttaaac gcttctgtcc cagcctcttt tttccttcct tcaatttttt acacgtcgac
    58861 gattcaataa aaaaccgcct aatattgtaa agacaggtgc aagaatcgag aaaaaggcga
    58921 atggcaagta atgcaacgcg ctaactttca tcgttcccat aatgaatgtg ccgctgactc
    58981 cccacggtac gagagaattg acggtcgcac ctgaatccgc tagtgttcgt gtcaaatagg
    59041 tttttgataa gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga agaatgatgg
    59101 acagatactg ttctccaacg atcagattga ttccgataca gctcaaggca gtaaacccga
    59161 ctaaacgaga aggtcggtcc atcttttctt ttaattcttg aacgatggta tcaacgatca
    59221 gatatttgat cagtaaccca cctaatgcca atgcaagtaa aatcaaacta gcagaaccta
    59281 acatactcat gatcccgcct cgagaaagca gttcatctag ctttggatca ggtgtatttg
    59341 cgacataacc attcatcaag atgtctgctg cttttccgac cgtcaaactt ttctcatgga
    59401 taaaagaaag aacgagagca gctgtcgatc ccgctagtaa tgtagggata gccggaattt
    59461 ttttgattgc ccccccaaat aaaataacta gtggaatcaa tgtccaagga gagatccaaa
    59521 agctcgcttt catgttttgg atcatttcat tgatactagc tagatcggct gaatgactgt
    59581 tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa tccgtataac
    59641 gcataccagc gatatgttcg aatagattaa ctcctccaat acccgctgcc aagttggtcg
    59701 tatctgataa aggcgagcag ttattcccta aaaaggcacc ggaaacgact gcgcctgtag
    59761 tcattgcatc attgaatcca agtaaatgac cgatccccaa aaaagcaatt ccaatcgtcg
    59821 aaatcgttgt aaatgaactg ccgacagtca ctccgacaat tgcacaaaca agaaatgctg
    59881 tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata aaccataatc gtagggatgg
    59941 ttccagataa aatccatgaa gcaactaaga caccaatcaa aagaaaaatc aggattggaa
    60001 tgatccctgg agtgattcct ttgatgatcc cgtcatggat ctcatcccat gtgaactttt
    60061 taaatctgcc ataaaacaac aaacacatga aagctgcaag gatcgggata tgtggcgaga
    60121 gctggaatcc gatgatcagt actcctagga gtgccaacaa cacgatcaat acagcgatac
    60181 tttcttttaa agaaatattt ttattcatcg tcgatctcct atttcaaatg aaaaggccct
    60241 gccatgaaaa aactattcac ttggcaggac ctcctactaa atcagcgaca aaacgtcggc
    60301 tgaaagactt atttttttat ttttcttcta aacttttttc tacctcaatc ccccaagaat
    60361 ctttcttgaa gtgattgatg atcaatggaa taacgaacat gaccactgtg atagctgcta
    60421 ctaataatgc atcactcaat gtcgctgtac ctaatgtagc aaatacgaca acgatgatag
    60481 agaagatcag catgcagctg actacccaag ctaatccgtt tccgttttta ccaattcggt
    60541 aaggacgttc gaattctgga tgtttcttac gcattttaag caatgaaaca gcaatcaaaa
    60601 tgtacacgat acaataaata acagttgtag catttgtaag tgtcaagaat acagcactta
    60661 cgtcatccat tacaccataa agcaaagcaa ataatgaaat aacgattgat tgtgttagaa
    60721 cgacatttct agatacgccg tatttgtttt ctttatggaa tccgaatttt ggtggaagga
    60781 atccttcacg agctacttgg atgattgttt ttgatggtcc agtaacccaa gctgataatt
    60841 gtaaaagcac accaataaat accatgaagc tgaagatatt tccgatcact tctggtaagc
    60901 ctaggatttg acagtcaatc aagataggct gcgtgatatt tgctaattcc atttttccat
    60961 ctggtacgac atttgctaca agcatcgcat tgatgacatt caataaaact aatccgatca
    61021 aagcgataaa tacgcctttt gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa
    61081 cagaactcat ttcgatccca acaaaaataa atgtgatacc agctaagtat ttcaatgaat
    61141 caatatgttc caagtcaggg aaagcttttg accatgaaaa gtcacctaga tagccgtttg
    61201 agactagtcc gactttgaag gctgccaaaa cacctaaaac gaacatgatg actactggga
    61261 tatatacccc tagccataca ccgatgttcc cgccgacttt tgccatatcg aatttcaagt
    61321 tcaagatcgt gatgatccag tagataacta aaatacaacc taaaacgaac cagtgattat
    61381 tgccaagttc tacattacca aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca
    61441 ttaccatacc tgggaacatt tgcacccata atagccatgc aacgacaaat ccccatttgc
    61501 tgcccagtgc tgtttttacc cataactgag gaccgccttc ttgaggaagc atcgtggaaa
    61561 gctcaccgga aaccattgat atcggaccgg caaaaaacaa aactgcaaaa attgaataaa
    61621 aaatcaatgt ccaaccgact gcggctaacg taggaatact ccgaaccgtt gcacacagtg
    61681 ccattgtcag accgataaag gtcccaagac ccattttact tacttcagat ttactcaccg
    61741 ttgacactct ccttgatttt ttggataaaa taacggtgtc tttgaagtag tttcagcaat
    61801 aaacgaaaca attcagcgaa tatttgaggg gattctttga gtttattgct tgaagggttt
    61861 cttcaaagac aacctcaaaa caaagggatc gaagagctta ggtctcccct tgctcttcag
    61921 atccaaacat gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg attaccttgc
    61981 ttattttgct tcgcttgcca ataattggtc tgcgtagatt ttttccaatt tttcttgaag
    62041 agctgctttg attttttcag catattcttc aaagtgttct tctttgttca tgtatggtgt
    62101 cattacgctg gcacgcaata cagttacttt tccggcacgg ttccattctt catctgagaa
    62161 tcctagttgg ttcacaaatt gtaatgggct gttgccatag tctgggatag caaagtcagt
    62221 atgtgaagtt aagaactcat taccgtaaat gcttcctttc acgtatgaag aataatcata
    62281 tacgtcatgg ttcaatttat tcattgcaac taagtcatcg ttgccttttt ctttgaatac
    62341 atagtctacc atattgaaat ctggataagt taatggatga acttcgattt ctttgtctcc
    62401 tactttaaat gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc
    62461 acccattaat ttaccatatc ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac
    62521 gctggctgct gttgcccctg cttttgaacc ttccaagata taagccccaa gtaatgctgg
    62581 gatatctgcg cctttttcaa atacatatgt cgcaaagtaa gagataacgt ctctcatgcg
    62641 gatatctttg atgacgatac caccggcaga atatggaaca tagcccattt tatgtgggtc
    62701 aatcgttaca gattctgctt cttcgattgc tttatatgca ctgtgtactt cttctaagat
    62761 ataatctttg ttttctgtaa agacattgta tttgtaatga acatctttta aatcttcaaa
    62821 tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc
    62881 atctacgtgt aagtagaagt aaataccgtc tttttctaat acgcgacgca atgcaacgat
    62941 tttatcgata ccgtcgattg ctccttcttc tgttgatcct acaacaccga ctacacctag
    63001 gattggtgtt ttttcagctg ctagaccacg aacgattttt tcaagttcgt tgatgtccat
    63061 gcgatagttg tggtcaactg gcactggaat cacttggtct aaaccgatac cgatgatgtc
    63121 ggcagctttt agccatgaat agtgtttagt ttgtggaacc aaccattttc ctaatttttc
    63181 taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt ctgggacgct
    63241 gtctaacaga ttcatgattt cttctgttga caagttcatt aattcccaat cgctttttcc
    63301 agcaactagt tcaggtgtca cttctttcat tgcaagcggc aatgatttga tgttgcgggc
    63361 ataccaaagt ccttctaagt ttgctaaaga accatcagca acgatgtggc cccagccatc
    63421 tttatagctc attaattttg caaattccat tcctacttct tcttccattt ggcttgttgc
    63481 aggagaagat tcataggcaa cgttattacc attccatagc atagcaaaat tgtacgctag
    63541 taaagatggc atcaatgttt cagagttcat atgaccccag taacgtccgg cattatgcca
    63601 aggaacggaa tgggtacgca tccgcgctga aatttctgat agaacatcct ttgttttatt
    63661 cacagtgtgt tcaaaactag cactgctttt ttcttctggc gtaataatag gcatgtcttg
    63721 aggcatgtag ttttgacgcc agcctaaatg ttcatccacc aattcattca acaaagcttt
    63781 atagatttgg ccgttctcag ctttgtcccc aatgaatagg gcatttaggt ttagatcttt
    63841 cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc
    63901 tacattacac ctgttcttct attctgtaaa ctaagttttg gcctgatttt taatcatatt
    63961 ttaatatgta tcaattatcc gaattgttca cacttttgaa ataattaaaa aaaacgaggg
    64021 aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt
    64081 aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc cttttttaag gtaaaaactt
    64141 actaaattct aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc
    64201 acaaacttac catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg
    64261 tcaccgttaa tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt
    64321 tcaatttttg tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg
    64381 ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca
    64441 gagaacaatg cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat
    64501 cttgtaactt cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact
    64561 tttttcgcta agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc
    64621 actacatcgc gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc
    64681 ttcggatcaa gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc
    64741 agcatcaatg ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt
    64801 aggtctaatc cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca
    64861 tcgtatgttt tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct
    64921 gtaaatgaga ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg
    64981 atgttgaagt ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag
    65041 tcgtagttgt tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg
    65101 gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg
    65161 cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg
    65221 cctgccaatt ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa
    65281 tcaccagttg gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga
    65341 agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata
    65401 ttcattttac tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatatgta
    65461 attaattatt tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa
    65521 gggacgaaat ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga
    65581 acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta
    65641 aagcatgtac tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact
    65701 tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc
    65761 tttgtcaaca gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc
    65821 tctaattccg aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt
    65881 ttctgcaatt tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa
    65941 ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa
    66001 aaattatttt aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt
    66061 ttgtcatccg atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa
    66121 aagcaaaaga accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc
    66181 aaagctacga ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta
    66241 ctgaaacaca acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt
    66301 ctgatcctac tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag
    66361 ccttccactc tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca
    66421 accgttcgat cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga
    66481 tccaaaaaga cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa
    66541 aaccattggt tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt
    66601 ttcctagccg attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag
    66661 ataaagaaga aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac
    66721 atcctgattt ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag
    66781 ttttaggtta tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga
    66841 ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc
    66901 atacatgggg tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg
    66961 aattgtggca gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat
    67021 ttggtgcaga catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact
    67081 tgaagagtca aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac
    67141 aattaggtac ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat
    67201 ctgatttaga cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg
    67261 acaaagaagc acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact
    67321 gacattttat cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct
    67381 tgatcaggac ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct
    67441 tagaaattcc ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca
    67501 aatccacttt tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt
    67561 cttgcgtata cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg
    67621 tcatcaaatc gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac
    67681 tcatggcaat cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt
    67741 gccctaaatc attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa
    67801 aatatttaaa aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca
    67861 ctccagcggt tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct
    67921 ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aattttaaac
    67981 ttttataata tgtatagttc aaatttattc tgaggtttat tataactatc tattgagaaa
    68041 gtatcaagct agcaattcct tgtttatgaa tt
//
LOCUS       DAJSVP010000007        65510 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSVP010000007 DAJSVP010000000
VERSION     DAJSVP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.007"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            170..802
                     /locus_tag="ON449_000417"
     CDS             170..802
                     /locus_tag="ON449_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3933039.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            925..1563
                     /locus_tag="ON449_000418"
     CDS             925..1563
                     /locus_tag="ON449_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3933040.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(1682..1754)
                     /locus_tag="ON449_000419"
     tRNA            complement(1682..1754)
                     /locus_tag="ON449_000419"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1719..1721),aa:Thr,seq:cgt)
     gene            1875..2117
                     /locus_tag="ON449_000420"
     CDS             1875..2117
                     /locus_tag="ON449_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933041.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2256..2885)
                     /gene="upp"
                     /locus_tag="ON449_000421"
     CDS             complement(2256..2885)
                     /gene="upp"
                     /locus_tag="ON449_000421"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3933042.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3019..3966)
                     /locus_tag="ON449_000422"
     CDS             complement(3019..3966)
                     /locus_tag="ON449_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3933043.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4119..5363)
                     /locus_tag="ON449_000423"
     CDS             complement(4119..5363)
                     /locus_tag="ON449_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3933044.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(5538..6563)
                     /locus_tag="ON449_000424"
     CDS             complement(5538..6563)
                     /locus_tag="ON449_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3933045.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(6585..6764)
                     /locus_tag="ON449_000425"
     CDS             complement(6585..6764)
                     /locus_tag="ON449_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933046.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(6757..7596)
                     /gene="prmC"
                     /locus_tag="ON449_000426"
     CDS             complement(6757..7596)
                     /gene="prmC"
                     /locus_tag="ON449_000426"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3933047.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(7589..8662)
                     /gene="prfA"
                     /locus_tag="ON449_000427"
     CDS             complement(7589..8662)
                     /gene="prfA"
                     /locus_tag="ON449_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3933048.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(8665..9249)
                     /locus_tag="ON449_000428"
     CDS             complement(8665..9249)
                     /locus_tag="ON449_000428"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3933049.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9355..9824)
                     /locus_tag="ON449_000429"
                     /pseudo
     CDS             complement(9355..9824)
                     /locus_tag="ON449_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9930..10532
                     /locus_tag="ON449_000430"
     CDS             9930..10532
                     /locus_tag="ON449_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3933050.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(10636..11205)
                     /gene="lepB"
                     /locus_tag="ON449_000431"
     CDS             complement(10636..11205)
                     /gene="lepB"
                     /locus_tag="ON449_000431"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3933051.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11435..12202)
                     /locus_tag="ON449_000432"
     CDS             complement(11435..12202)
                     /locus_tag="ON449_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933052.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12199..12678)
                     /locus_tag="ON449_000433"
     CDS             complement(12199..12678)
                     /locus_tag="ON449_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3933053.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(12803..13540)
                     /locus_tag="ON449_000434"
     CDS             complement(12803..13540)
                     /locus_tag="ON449_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3933054.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            13701..15101
                     /locus_tag="ON449_000435"
     CDS             13701..15101
                     /locus_tag="ON449_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3933055.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15324..16676
                     /locus_tag="ON449_000436"
     CDS             15324..16676
                     /locus_tag="ON449_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3933056.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            16669..17367
                     /locus_tag="ON449_000437"
     CDS             16669..17367
                     /locus_tag="ON449_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3933057.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17429..18580)
                     /locus_tag="ON449_000438"
     CDS             complement(17429..18580)
                     /locus_tag="ON449_000438"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3933058.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(18593..19030)
                     /locus_tag="ON449_000439"
     CDS             complement(18593..19030)
                     /locus_tag="ON449_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3933059.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19045..20811)
                     /locus_tag="ON449_000440"
     CDS             complement(19045..20811)
                     /locus_tag="ON449_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3933060.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21075..21752
                     /locus_tag="ON449_000441"
     CDS             21075..21752
                     /locus_tag="ON449_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933061.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            21755..22882
                     /locus_tag="ON449_000442"
     CDS             21755..22882
                     /locus_tag="ON449_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933062.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23082..23522)
                     /locus_tag="ON449_000443"
     CDS             complement(23082..23522)
                     /locus_tag="ON449_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3933063.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(23533..25566)
                     /locus_tag="ON449_000444"
     CDS             complement(23533..25566)
                     /locus_tag="ON449_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3933064.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(25568..27271)
                     /locus_tag="ON449_000445"
     CDS             complement(25568..27271)
                     /locus_tag="ON449_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3933065.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27365..28315)
                     /gene="manA"
                     /locus_tag="ON449_000446"
     CDS             complement(27365..28315)
                     /gene="manA"
                     /locus_tag="ON449_000446"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3933066.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28354..29379)
                     /locus_tag="ON449_000447"
     CDS             complement(28354..29379)
                     /locus_tag="ON449_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3933067.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(29588..30457)
                     /locus_tag="ON449_000448"
     CDS             complement(29588..30457)
                     /locus_tag="ON449_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3933068.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31161..33053)
                     /locus_tag="ON449_000449"
     CDS             complement(31161..33053)
                     /locus_tag="ON449_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933069.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33050..34777)
                     /locus_tag="ON449_000450"
     CDS             complement(33050..34777)
                     /locus_tag="ON449_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933070.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(34793..35443)
                     /locus_tag="ON449_000451"
     CDS             complement(34793..35443)
                     /locus_tag="ON449_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3933071.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            35647..35943
                     /locus_tag="ON449_000452"
     CDS             35647..35943
                     /locus_tag="ON449_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3933072.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36135..36755
                     /locus_tag="ON449_000453"
     CDS             36135..36755
                     /locus_tag="ON449_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3933073.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(36847..38013)
                     /locus_tag="ON449_000454"
     CDS             complement(36847..38013)
                     /locus_tag="ON449_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3933074.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38025..38378)
                     /locus_tag="ON449_000455"
     CDS             complement(38025..38378)
                     /locus_tag="ON449_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3933075.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38506..39528
                     /locus_tag="ON449_000456"
     CDS             38506..39528
                     /locus_tag="ON449_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3933076.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            39547..39708
                     /locus_tag="ON449_000457"
     CDS             39547..39708
                     /locus_tag="ON449_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933077.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(39967..40401)
                     /locus_tag="ON449_000458"
     CDS             complement(39967..40401)
                     /locus_tag="ON449_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933078.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40511..42370)
                     /locus_tag="ON449_000459"
     CDS             complement(40511..42370)
                     /locus_tag="ON449_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3933079.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(42488..43648)
                     /locus_tag="ON449_000460"
     CDS             complement(42488..43648)
                     /locus_tag="ON449_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR3933080.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43955..45379)
                     /gene="araA"
                     /locus_tag="ON449_000461"
     CDS             complement(43955..45379)
                     /gene="araA"
                     /locus_tag="ON449_000461"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3933081.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45395..46093)
                     /locus_tag="ON449_000462"
     CDS             complement(45395..46093)
                     /locus_tag="ON449_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3933082.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46050..47711)
                     /locus_tag="ON449_000463"
     CDS             complement(46050..47711)
                     /locus_tag="ON449_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR3933083.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47929..49329
                     /locus_tag="ON449_000464"
     CDS             47929..49329
                     /locus_tag="ON449_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR3933084.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(49581..50660)
                     /locus_tag="ON449_000465"
     CDS             complement(49581..50660)
                     /locus_tag="ON449_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3933085.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(50746..51705)
                     /locus_tag="ON449_000466"
     CDS             complement(50746..51705)
                     /locus_tag="ON449_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3933086.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(51717..53228)
                     /locus_tag="ON449_000467"
     CDS             complement(51717..53228)
                     /locus_tag="ON449_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3933087.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53250..53813)
                     /locus_tag="ON449_000468"
     CDS             complement(53250..53813)
                     /locus_tag="ON449_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3933088.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(53819..54682)
                     /locus_tag="ON449_000469"
     CDS             complement(53819..54682)
                     /locus_tag="ON449_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933089.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(54696..55592)
                     /locus_tag="ON449_000470"
     CDS             complement(54696..55592)
                     /locus_tag="ON449_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933090.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(55767..57056)
                     /locus_tag="ON449_000471"
     CDS             complement(55767..57056)
                     /locus_tag="ON449_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3933091.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57028..58545)
                     /locus_tag="ON449_000472"
     CDS             complement(57028..58545)
                     /locus_tag="ON449_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3933092.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            58743..59633
                     /locus_tag="ON449_000473"
     CDS             58743..59633
                     /locus_tag="ON449_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3933093.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(59706..60530)
                     /gene="nadE"
                     /locus_tag="ON449_000474"
     CDS             complement(59706..60530)
                     /gene="nadE"
                     /locus_tag="ON449_000474"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3933094.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(60542..62014)
                     /locus_tag="ON449_000475"
     CDS             complement(60542..62014)
                     /locus_tag="ON449_000475"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3933095.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(62651..63013)
                     /locus_tag="ON449_000476"
     CDS             complement(62651..63013)
                     /locus_tag="ON449_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3933096.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63236..63988
                     /locus_tag="ON449_000477"
     CDS             63236..63988
                     /locus_tag="ON449_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3933097.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64128..65051)
                     /locus_tag="ON449_000478"
     CDS             complement(64128..65051)
                     /locus_tag="ON449_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933098.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65066..65338)
                     /locus_tag="ON449_000479"
     CDS             complement(65066..65338)
                     /locus_tag="ON449_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933099.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18936 a        13430 c        11411 g        21733 t
ORIGIN      
        1 tttattagaa ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga
       61 tagcgacact atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt
      121 tggtttagct tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc
      181 taaagttaat gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga
      241 ttttgaactg tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca
      301 tccaattatt tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag
      361 aatggagatt accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa
      421 aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga
      481 aagttatatt gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg
      541 ttcaaacata aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga
      601 ctgttccctc actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga
      661 tactgattta aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc
      721 ggaacatcta aaaaattgta cgcttaatca gttgcaagca cttggttttg ctagaaaatt
      781 tttacaaata aaagtagtat aatcaaaatg aatatataag ataaattttt agcaatacaa
      841 atatccataa aaaaacattg cctattttaa ctcttcatgt atactgactc tatcttcaaa
      901 aactgaatag taggagttat tatcatgaat aacattttag gaaaaaaaat agaacttaaa
      961 aaagggatat tgggcactat tgtcgttgta ctcggtttgt tagggttgct atttgcgcca
     1021 tccattatta gcattttttc tttaactatc actggattta tagcatttac acttgttttt
     1081 ggtaaaaaag atacgaaaaa aatgttctcg aaacctgtcg ctcctgtcaa aaatatagcc
     1141 aaatattttt ttatcaatgt agtaatcagt gccgcagtct cggtattttt gcaacaaatt
     1201 ttgaaatggg gtttaacagg caatccaata aatgaggctt tcagcccgtt attatttatt
     1261 attttgccta tcatgatact tggagaagaa ttgttctcag tgtattttct cgctattttt
     1321 tctagtaagt tcagcattcc tgtcgctagt ttcttgtcag caatcatctt tggatttatc
     1381 cattacagta cttatgataa tggaaatatt ttacacactg tagcacacat ccttttgata
     1441 caaggggtgg caagattgtt attcaaccaa gctgccatta aaagcaactc gatcattaca
     1501 agttgggcag ttcatgtgat atttgatttc acagctatat tgctagtcgt tttattctcc
     1561 taaattttta tagaaataat acatgctcta ttgctaagaa agcatacttt atttccttta
     1621 aacaaaaaga cagatgtatc aatggattaa aaatccttga tatatctgtc ttctctttta
     1681 atgccagcta tagggatcga acctacgacc tacgcgttac gagtgcgttg ctctaccaac
     1741 tgagctaagc tggccttatc caaaagaaat tatatactct atttggctga ttgtaaagag
     1801 tccgatggaa aattagttta atttcatgat atttttcata aaacgtggta taattgatgt
     1861 gaaaggcagg ttttatgaaa atggcaactt ttggaaaatt tgatacaact atcgtaccat
     1921 cggaaattgg tgaaaaattc aagatcaata ctcacgtaat gttcacaatt cacggcaagt
     1981 cgcaaactgg aacgatttca aagcaattga aaaattccgc tgttgttgag attgatgaaa
     2041 ctgcagaaaa tgcgaacttg atgacgcaaa gtaagggtgt agtaatcgtt aactacaaag
     2101 aactaaaaaa gatttgaatt tgaccatctc tgcatttttg ggatggtttt tttatatgca
     2161 aacgaaagct gtgaaaaagc tgttctgcat caaacgttaa aaatgaccaa aaaaggatgt
     2221 gacaaaagtt gtgtcacacc ctttttcgta tttacttatt ttgtaccaaa caaacggtca
     2281 cccgcatccc ctagtccagg aacgatataa ccatgttcat tcaatttttc atctagagaa
     2341 gctgtataga tgtcaatatc aggatgcgct tcttgcaaag cttttactcc ctcaggtgct
     2401 gctaccaaac aaacaaattt aatgttcgac gcgccacgtt ctttcaatgc atcgattgcc
     2461 atgattgctg atccgcctgt tgccaacatt ggatctacta caaacaactg acgtgcatca
     2521 atgtcttctg gaagtttgac gaaatattca tgtggttcca atgtttcttc atctcgataa
     2581 agaccgatat gtcccacttt agcagctgga atcaattcca agataccgtc aaccattcca
     2641 atacctgcgc gcaagattgg aataatcgct acttttttac cagacaacgt tttttgtgtt
     2701 gtttcagtga tcggtgtttc gatcacgatg tcttccaatg gcatatcccg tgatacttca
     2761 tatgccatca acatagcgat ttcatcgact acttcacgaa aaacctttgt tccgcagttt
     2821 ttgtcccgaa taatagttag tttgtgttga atcaatggat gatcgatcac ttgaaatttg
     2881 cccatttttt aagctctcct ttgttctgtc gtttcttttt aattgtaaaa caaaatcaag
     2941 tgattgacta gtcaaactcg ccatttttcc taagaaaatc cgtttttgaa aagaagtttt
     3001 cctgcggaca agcaaagctt attctttttt cttgatccaa gaagtagata atgccattgc
     3061 tgcacgcgtt gcctttgtct gatccatagc atttaccatg acaaaatcat ggatgattcc
     3121 ttggaagcgt acttgtgtaa caggaacccc tgcatcacga agtttgcgcg cataagcctc
     3181 accttcatct cttaatacat ccgcttctcc cgttaaaatc attgctgaag gaagatcatt
     3241 tagttcattt atatttgctc tcaaaggcga agctgtgatt tcagcacgct cttcttcatt
     3301 cgtcgtatat tggtcccaaa accacatcat tccttctctt gtcaaaaaat agttttctcc
     3361 gaattcatga taggaatctg tatcaaaatc agcgtttgtg actggataat acagcaattg
     3421 cttggatatt gggaggccct ttcgttcttt tgttaagatc gtcatgactg tagccatgtt
     3481 accacctact gaatctcctg caacgattag attctcgaga tccagatttt ttgacttggc
     3541 taatttattc agctgttgca acacagcata attttgttca attgctgttg gatattttgc
     3601 ttctggtgat cgactatatt ctggaaagac aacgacggaa ttcgtacgaa ccgcaagttc
     3661 tctgatcaat ttatcatggg tttgagcact gccaaaaacc cagcctgctc catgaatata
     3721 aaaaataact ggtaatttat ccatattccc ttctggtcgt acgagacgaa tattgagttt
     3781 tccccattcg ccagtatcca ccatcaagtc ttcaatatcg acggaatatt tgttcacagg
     3841 tgattcttgt acttcttcta gtaaaactct tccttcttcc ggtgctagtt catagatacg
     3901 cggatgaggc gcatttgcat cactgaatgc tactgcttcc ttttctaacg agatacgatc
     3961 agtcatgact tttcctccct gagatttagt gtgaagacta aaattacacc tcatatacta
     4021 gagagcatca ccgatattgt caaagaatcc gttttcccaa aaaataaaca gaaaagccca
     4081 acattgaact tttctgttta ctaatggaat ttaaattttt attttttatg caacggaaat
     4141 ttttcagtta attcgcgtac acctgttttg acttcgtcta gttgagcgtt atcatctttt
     4201 gcttgtaatg ctttgacaac tagttcagcc acttttacag catcttcttc tttgaacccg
     4261 cgagtagtga ttgctggcgt gccgattcga ataccgcttg ttttgaatgg acttagtgtt
     4321 tcaaaaggaa tcgagttttt atttacagtg atattcacac tgtccaaaat gctctctgct
     4381 tcttttccat ttatacctaa ctcgcgaaca tcgatcaaca tcagatgatt atctgtcgcc
     4441 cctgaaatga ctctggttcc aattgcttga ttgaaaactt tgaccattgc ttttgcattg
     4501 gcgatgattt gttcactata ttctttaaat gcaggatcta atgcttcttt gaatgctact
     4561 gctttacccg caatcacatg ctctagaggg ccgccttgaa taccaggaaa taccgcacta
     4621 tttatttttt ttgctaatgc ttcatcgttt gtcaaaatca tgccgcctcg agggccacgc
     4681 aatgttttat gagtggtagt cgttgtgata tctgcataag gaactggatt aggatgaagt
     4741 cctgctgcta ctaatcctgc gatatgtgcc atatctacca tcagttttgc gcctacttca
     4801 tctgcgattt ctctgaattt cgcaaagtcg atcgttcgtc cgtaagcact ggcacctgct
     4861 acgatcaatt ttggttgatg ttttctagct aaaatacgta ccacattata atcaataact
     4921 tctgttgtcg gatcaacacc gtaagcaaca aagtgatagg ttttgccact gaagttgact
     4981 ggtgatccat gtgttagatg tccgcctgcg gaaagatcca ttcctaagat cgtgtcccct
     5041 ggttctacca aagcgagata agcagccgtg ttggcttgag agccggagtg aggctggacg
     5101 ttcgcaaatt tcgctccgaa taattctttt gcacggtcaa tcgctaaatt ttcaacgata
     5161 tcgacaaatt cacacccacc ataatagcga tgccctggat aaccttctgc gtatttattt
     5221 gtcaagatac ttccttgtgc agccatcact gcttcggaga caaagttttc agaggcaatc
     5281 agctccaaat tatgttcttg tctttcttct tctttggcga tcgcagccca aagatcaggg
     5341 tcaaacgttt tgtaatctac cacaaacaac agctcctttt tattttccta caattttatt
     5401 tttcccatca aaaacaaatt gccaaatatt cgtatctctt ctattacgat tagttacgct
     5461 tagtttttcg ttcactgata gaagatttac ttgatagcat tgtatcatac taaaagaatg
     5521 cgacaataac caattgatca tttttcaaaa taattttgtc ctgctgcttt tttcaagcga
     5581 ttcatgtaag cattccccaa tccagtttct ggatacactt gtacaaaaat caaatctaat
     5641 ccaagtgttg gttcatctaa tcctcttaga cctgcaaaca atcctttagc cgccgcttct
     5701 actgagttgt cgttatacat ataaacagct gcagtatctg tccgcacttg atccgcaatt
     5761 tcggggcttg ctatgacacc tgcacggatc ttcttatttt ttgcccattg aactgcagtt
     5821 gaccaatcgc catctctgac catcagaaca cgtgtatcgg gtgagtagtg tttgtatttc
     5881 atcccagggg cttttggtgt ttcattttct tttacaagat gctgatcaat agcaacagga
     5941 gattcgatga ctgcttcgat ttgttctttc gttactgcac ctggtcgtaa aatcataggc
     6001 atggctgttg gatcacttaa atcaagaaca gtggattcta caccaattcg cgtagctcca
     6061 tcatcaataa tcccggtaat ttttccttgt agatcatgat atacatgatc tgcagtcgtt
     6121 ggacttggtt ttcccgacgt atttgcactt ggaccgacaa gcggaacacc ggaaagttcg
     6181 attacttcta gtgttttttt gttatccggt atacgaaatg cagctgtgga taatcctccc
     6241 gtcacaactg agggcaaagt gtcttttttg atcttaaata tcaaagttaa tggtccaggc
     6301 cagaagttct tcacaatctt ctctgctgcc ggatgaaagt tgtccacata ctcttttact
     6361 tgttcaaaag aggcgacatg gacgattaat ggattatctt gcggtctccc ttttacagaa
     6421 aacactttct tgacagcgtt tggtaaaaga gcgttggcgc ccaatccgta tactgtttcg
     6481 gtaggaaaag caacaagttc tccttctttc agtgcttcgg ctgcctcttg caattctgtt
     6541 tcatcatatc gtttcgtttc cacagtttat ccacaacccc tttttcatac attcttattt
     6601 tgtctgagtt tatcctcaag ttttccactg tttgtggata atctatttgt caatagtgga
     6661 taaacttgtt gataaaccaa aaagtcgttt acatccttgt caaataagtg tttttttata
     6721 ataaaaaaac aaataaaaaa tttttcatcc acaacttcaa ccataaactt ggatcattcg
     6781 ttcattgcca aaaagatcct tttttatcgc cactttttta tctggaaatg cttgctgaaa
     6841 tatttttttt actgcttcac cttggcgaaa tccgatttct aaaaaaatct ttccatttga
     6901 ttttagtacg cttggtgctt caactgctat tttttcataa atagctaatc catcgttctc
     6961 agcaaaaagg gccattttag gttcgaatga ccggacactt tcatccatca gttcccattc
     7021 attccgactg atgtacggcg gattagaaat aatcacatca aattgctgat ccattactgg
     7081 tcctaatgta tctccatgat aaaaactgat tttcgttcct tctttctgag cattttcttt
     7141 cgctacttct aatgcttctt ctgaaagatc cacagcagat atatgccaat tttttcttgc
     7201 tgctttcaaa ctaatagcaa ttgctccagt ccctgtccca atatctatca cttctaacgg
     7261 tacaccaggc gtctcgtcta aacaccattc gactaactcc tctgtttctg gtcgagggat
     7321 cagtgttgct tcagttacct ttaaccgatg atcataaaaa tctgcatatc ctagcaaata
     7381 ctgaggtgga tgatctgcaa gcagaagtcg cagatcggtt tcgatcaacc gctgttcttc
     7441 ttctgaaatc tcttcgttca tatgaaggag ccaatcaagt tttttccatt gttttcgttc
     7501 caaaaataaa aattggatac tatatgcttc tttcccatga tcctctaaaa aagaagaagc
     7561 ccgtgacagg acttctcgat aggtcatttt acccattttg catagcttcc agttttgatg
     7621 tttgatcata catgatcaat gcatcaacga tctcatctaa tttccctgct aaaatctgat
     7681 ctaatttttg gatagttaat ccgatacgat gatcagtcac acgattttgc gggaaattat
     7741 acgtccggat ccgttctgaa cgatccccag ttccgactgc tgattttcga ttggcatcat
     7801 actcgctttg tgcttcttgc tggatcttat cgtacacacg agcacgcaag attttcattg
     7861 ctttttcacg gttcttgatt tgagaacgtt catcttgcat agcaacaacg atccctgttg
     7921 gtaagtgagt caaacgtaca gcagatgctg ttttattgac gtgctgtccg ccagcacctg
     7981 aagcatgata gatatctgta cggatatctt tgtcttcaat atcgatctct acttcttcag
     8041 cttctggcat cacgacaact gttgcggtgg aagtatggat acgcccttgc gattcagttg
     8101 aaggaacacg ctggacgcgg tgcgcaccac tttcatattt taatttcgaa tatacgttgt
     8161 ctccggtgat catcatgatc acttctttat atccaccgat tccggtaacg ttagcttcca
     8221 tcacttctgt tttccagcct tgtgcttcag catacttttg gtacatattg aaaagatcgc
     8281 cagcgaataa tgctgcttcg tctccgcctg ctgctccacg gatttccatg atgatatttt
     8341 tatcgtcatt tggatctttt ggtaaaagca agatcttgat tttttcttct aagacttctt
     8401 tttctttttt taattcagat aattcttctt ttgctagttc agccatttca tcatccagtt
     8461 tttcaccaag cagttcttct gtatcggtga ttccttgtac tacttcttta taacgacgat
     8521 aaacttcgac tgtttcgcgc gtgtttgctt cttcttttga aagctgcata aaacgctttg
     8581 tatcagaaat cacttctggg tcacttaaaa gttctccaag ctcttcataa cggtcttcaa
     8641 ttgcttgtaa ttgatcgtac atgcttattc tccttctgta atcattgatg ggtgaaagta
     8701 atgttttcga caaacggggt aataagcttc atttccgcca atttgcactt gatcgccttc
     8761 ataaacaggg tttccatcaa tataatgtaa attcatgatt gcttttttgt ggcagaacca
     8821 acagattgtt ttcatttctt cgattttgtc tgcataaagc aataaatatt ttgatccttc
     8881 aaataactcg tttcgaaaat catttttcaa accaaatgcc ataaccggaa tatccaattc
     8941 gtccacaatt ctcgtaaaag caatgacatg tttctttttt aagaattgcg cttcatcgat
     9001 cagcacacaa tagggtttgc tttccatttt ctgaattacg tcaaatacgt ctgtttcttc
     9061 aaatatcggc atcgcattac gtttcagtcc gattcggctt gaaacaacgc ctaaaccatc
     9121 tcttgtatca ataccgcttg tcattaagac aactggctta ttttgttctt cataattatg
     9181 tgcgactttt aggatctcga tcgtcttgcc gctattcatt gcgccatact taaaaaatag
     9241 ttgtgccatg ctctttttca ccttccagtt tgttcctatg gtattatact tgattttcag
     9301 cattttttac agtgagaatt ttcatcaagc acagattagt attaaatcta ttgctcactt
     9361 ttgttccaat tttttgacaa gaactaaaca tgggttccat tcatcccata aatttggaaa
     9421 aacttccaac cttgaaaatc ctaatgattc gtagaaacaa atagtttgat catatataga
     9481 gtagtgcccc tcgtctacag tcttcacttg caaatatttg taatgttttg ctgcgtaacc
     9541 ttctgcttct ttaaaaagtt ttgttcctaa tccttggtga tgatagtctt tttttattcc
     9601 catacaatag aatagatttc ggcagtatct tcacttgtct gtgaaagagt gataaagcct
     9661 aaatattctt cttttctttt ttcacaccat aaaggtagtt tacttgattc ctcgatatat
     9721 ttttccgttg attcaggaag cccaaaccac tcaggcaagt ctagtaatat ttctcggaca
     9781 cactttcctt tttcctctct atcttttaca aacgatatcg gcatattctt tctagattct
     9841 ccctttttga attgagtata gcatgctttc tttcttttca cgtgctataa taagttaaaa
     9901 tttcaaagtt ttacgtaagg agctatttga tgacaaaaaa tatttttgat tccatcgcta
     9961 accattatga ttcccctgac cgtcaagcat tagccaagat tattcgcgaa gaactaacta
    10021 cttatctacc gcgcgacagc caccaaaaag tcctgctgga ttacggaggc ggaactgggc
    10081 ttgttagttt accgcttgcg gaaagattca aagaactgat cattgccgat gcttcagaaa
    10141 ctatgctgaa aatggcagaa gaaaagatcc aagcagcaga tctgaaaaat gtccgaacga
    10201 tccacgctga tgcttctgtc gaatttcctg ctgtccaagc taatctcatc cttctatcac
    10261 ttgtactttt acacattcct gatacggaga atattcttac aaaactttat gaaattttag
    10321 ctcctggcgg tcaattgatc atcgtagact ttgacaagaa tgaacaaatt agtcatccga
    10381 aagtccataa tggctttagc caagaagaat tgaataatcg attggaaaaa acgggctttg
    10441 tctctgctac tagtcatacc ttccatcgtg gggaaaagct atttatgaac aaaaacgctt
    10501 cgcttttctt atccatcagc caaaaagctt aaaatgagat tgcaaaaaaa gtgagcaact
    10561 ccaagaaata agaaggaatt ttcggaaaaa gaagctgtga caaaaaacat gtcacagctt
    10621 ctttttattt tttcatcaca tgattttaat ttctgaaaga ggccaataac ggaaaacaac
    10681 ttttccttgg atcatatctt tgtcgactaa cccaaacatt cggctgtctt ttgaaatcaa
    10741 gcggttatcg cctagaacaa gatactttcc ttttggcaca gtatattcaa agtcactcgt
    10801 aaaggtggat gaactttctg cttgtgcttg gaatcctgag ctataagcat attctccttg
    10861 taacttatct tctgaaaatt cttttttgaa ctcatccaga taaggttcat catacttttt
    10921 gccattaatg gtcaactggt cattttccat cttgactgta tctccaggca taccgatgat
    10981 ccgtttaaca atcatccgtt cttcatcccc cggttccttt gtagtaataa tatccattcg
    11041 ttcatagttt gagatcttag atgaaatcaa tcgttgtcca tcacttaatg tagggtccat
    11101 cgaatgcccg tttacagtaa ctggtgtaaa cacgtacact ctagctaata aaatagccgc
    11161 taatacaagg atcaaaatcc cccaattttt caaaaattct ttcacagctt acctcctact
    11221 tgtattttcc tcgttattta ttacgttttt tcacaatact tcgttgtact ttctttattc
    11281 tacaatgttt ataaatgaaa ggaaagaata agtgatgtgt tcgtcttatc tttctcgtta
    11341 tataaaaaag gaacgtgaca aaagacgtgt cacatccccc ttattccgaa taaacgtttt
    11401 tatcgcaacc actgatacac ggcgtttcat cacattatga atgtcttctt ctttggctac
    11461 gactataaac cactggttct tcctgcgact gtgccttttt tgcaatcaat ttcagacgat
    11521 aaggctcatg atagcgtaac ccgtctttga caaaatcacc gccgacgatg aacaatccga
    11581 caggttccaa tgtgtcttct gttgagtcaa gtcgaatggt ccctactttt tccatgtccg
    11641 agtagcttcg tccaatattt agaacatact ctttctctcc tgttttcttg acatagtaat
    11701 aaggatacaa tgtatttcta tcgaccagaa atccatccat gcgaccgagt tcatttgttt
    11761 tgatctcacc atttccaatc aaataccgca tattctcggc taatttcaat tgataatctt
    11821 cgtcataaga aaccatgacc tcttgtgtat tcactttatc gatcgaattt ccattatcta
    11881 tcgattcttc tgtctggatg ttgaacgctt catcaggtaa atacttcaca aaaagctgag
    11941 tgacatcggc tttcgtgttt ttatctaaaa agtaataaaa tacatttaaa acagtaaaat
    12001 aaacaagcac tagaaatgca agtccatata agaaaaattg aagatagttt tggttttgcc
    12061 aaagtccata aacaattctc aataaataaa gcgtgggaat cacacttaaa atcgtatatc
    12121 cacggttaag atatttagga ctgatgtcta aatagccaag gaatttatgc accccgttga
    12181 taaggtctag taaaaatgtc attgagaatc acctgcttgc tggttatacg tttgggtatt
    12241 tcctgaatct tggttttgct gtgctgtacg tgctgcatca tcattttgcg tgttatcatc
    12301 tacagtcgta ctgcttggta tcgtttcgga gctagcactt gtatccggac ttgtacttgg
    12361 attggttgaa tccgatggag tgccatctgg taccgtatta cttgtttcgc ttgagctgtc
    12421 agttgtatat gtctcatcat acgacgaact agttgttgta tcgtcccatg tacttgtcgt
    12481 agtggtttca ttcaaacttt ccgttgttgt tggttcagat gtcgagactg aagtagttgg
    12541 tgttgtttct tctacagtac tagaacttga acttggtgca cggtagctgg aactattcac
    12601 aatacctgta gtagtcgcga caatcgtcga gattgttaat acagcgattg gcttcaaaat
    12661 aggtgctact tttcgcatct tatctcttcc ttcattgaaa tttattcttg tttccttact
    12721 ttactacgga caaattatac agtctacctt atttctacca taaattcttt atttttttct
    12781 tacgtttttc cgaaagattt ctttaatttt cctcaaaaac tacggttgat gtttttaagt
    12841 aagcttttgc ttcccgattg atttctttca gatcgcatcc ttgctgcttg gcagcgaaca
    12901 cgcaaccaca ataacattgg cgataaatat catactcttt gcacatttgc aaagagcgtt
    12961 catagccttt gtttttcttg aaatcacttg gcaagtattg tgtcccgtaa atcttttgga
    13021 tatccattcc gatctgattg atcaaagctg catttttctt tggcgagatg gtcagggcac
    13081 ttccaaagta atcgtatcct agttcttgcg ctttttgcgc aacaagatct aatcgcatat
    13141 tgaagcatgc actgcagcga gcaccacctt ctttttcgtt ttcaagatgg tttgttaaaa
    13201 ccattttggt gaattcattt ggacggtatt catcaacgat tagcgcaaca tgatggtcag
    13261 ttttctcatt aaattggtct acaaatcgtt tttgttcatg taatcttcgc aaatactcac
    13321 ttttaggatg aatattcgaa tttgaaaaaa ataacgtgat ctctgcatat tgagacatga
    13381 attccaatgt gtgcgtactg caaggagcac agcatgtatg tattaatatc ttaggcttta
    13441 cttcttcttt ctcccattgc atgatcattt ttttcagaac tgtatcataa tttatttttt
    13501 gatttttcat tttttctacg atttgttgag cattaatcaa tttgttattc ctcctccttc
    13561 ttttttctag gatttgcctt attataagcc atttctattt taatcgaaag ctgtattgtt
    13621 atctgtttga taatttcttt gagtattcct aattcccctt gtatactggt agaaacgact
    13681 attgtcagga ggatagtact gtgcatgttg atttattctt tcgaaggctg cagcggcgag
    13741 gacagaagtt tgctgccaat catttctcat cgatccaaat catcgttttt tattatatat
    13801 tgatgacggt cttatcactc gttctatttt atatgccgat ttttcgcgaa ccagatagcc
    13861 atgtttcttt tgtggacatg ctgttcatgg caatcagtac agtgagtgtg actggactat
    13921 cgacttttga tatcaattct gtgttcaatg ataacggtat tatactgctg gaaatattat
    13981 ttcaagttgg tggtttgggg atcatgatga tttctactgc ttttgtcatt ttttctaaac
    14041 gacggatcac actgaaacag cgacagctga tcatgacgga tatgaatcaa ccgcgtttgt
    14101 ctggtatcgt ccgactgatt cggatcactt ttgctatttt aatctggttt caattacttt
    14161 ttgggacatt cttttctatt tatttttatt acagaggcta ttttgatcgt tggcgtgatg
    14221 ccgtttttta cggtttttat caggcgatat cggctgtcac gaactctggg tttgacgtca
    14281 ctggggattc catcaagccc tttgcacatg actatttttt cttgatcctg atcatgtttt
    14341 tgatttttgt cggaggaatt ggttttcctg tattgatgga gtgccgggaa tggctattat
    14401 acaaacgttc caatgcgaag cttccgtttc gtttttcctt gtttaccaaa ttagctgtgt
    14461 tagcttttgt tatcttgttt gtttcgggaa ctgtcttgat ttatctattg gaaaaggacc
    14521 atttattcca agattccaat atgagtgtca aatggatcaa ttcgatgttt tattcgatta
    14581 cgactcgtaa tgctggtctg cagattcacg atttgggtga ttttcaaatc acaacactga
    14641 tcattttttc tttactgatg tttatcggat gcagtcctag ttctgtaggc ggcgggattc
    14701 gaacaactac tgtagcaatc atcggtttgt atctatattc cttcctcaaa agtgaagata
    14761 acatcaatat ttttggacgg agaatcgatc aagatgatgt gcgaaaatca gtcgttgtct
    14821 ttatgctttc tcttggtatg tgtttctttt gtatcgtttt tttatctgct acagaagaac
    14881 aaacgctgat ctcgatcatc atcgaagtaa cttctgcgtt tggaacaact ggtttatctt
    14941 tagggattac cggagacttg tctgttgtgg gcaaaatcac gattgctaca ctgatgttta
    15001 ttgggcggat cggaatgcta tatacattga tgttattcgt gccaaaagaa acacgagatc
    15061 taggctatga atatccaagc gagaaaatta ttatcggtta aaatgggaat ataggtaaga
    15121 gggaataata aaatcacagc agcttgaaac aatcattttg tttcaagctg tttttttgtt
    15181 aaatcatgcg attttgtccg atttcatgca ctttcagccg taaccttttc ttaagaataa
    15241 gaaaagcaat ttaacctttt tttcaacata tggtatgatt agttgaattt attcgtcagc
    15301 aaatcgtttt tggaggttac ataatgggaa tacgcagtca ttttgcgatt gcagttggaa
    15361 aaacgtcgca atgggcatta aaaacttttt ttaaaggcgg atcaagttta ccaggtaaac
    15421 tagcgctgaa agtcgatcca catatactag atagtttggc aaaagactac caagtcgtcg
    15481 ttgtcaccgg aacaaacggt aaaacattga ctaccgcttt aacagtcaat attttgcgcc
    15541 agcaattcga tgaagttcta accaacccta ctggtgcgaa tatggtacag ggaattgtat
    15601 cgactttctt gcaggcaaaa ccaaaaaaag gacagaaaaa gtttgctgtc ctagaaatag
    15661 atgaagcaag tttgagcaaa gtcacagaat atatgaaacc agaactgttc ttatttacaa
    15721 atattttccg tgatcaaatg gatcggtatg gagaaatcta tacgacctat aaaatgattg
    15781 tagacggtgc cgcaaagtca ccaaatgcaa cgattatcag taacggtgat tcaccaatct
    15841 tcaattctat cgaaacagtc aatcctagaa aatattatgg ctttgaccat gaagaagatc
    15901 gtgaacaaat ggctcattac aatacagacg gtgtcttatg tccaaattgt catcacatct
    15961 tgcattataa aatgatcact tatgcaaact tagggaaata ttactgtcca aattgtgact
    16021 tcaaacgtcc tgaattggat tatcgattaa ctgatatggt acggatggac aatgtgtcag
    16081 cagatttcgt gatcgatggt catgaatacg gaatcgaagt tggcggtatg tataatgtct
    16141 acaatgcttt agcagctact gcagtggcag agtacttcaa tgtcgatcct gataaaatca
    16201 aacaaggttt aggatatgac gagaaagtat tcggccgaca agaagtcatc aatatcaatg
    16261 ggaaaaaatg tacattagta cttgtcaaaa atcctgtagg tatcaatcaa gtaatcgata
    16321 tgattggtct agctccttat ccattttctt tagtggcttt attaaatgcc aattacgcag
    16381 acggaatcga tgtcagctgg atttgggatg gtaattttga aaactttgcc gatatggata
    16441 tcccagctgt tatttctgga ggagaccgtc atacagatat gactttacgt ctaaaagttg
    16501 ccgggattcc agaaaataaa ttgacagaaa caaaagaatt accagaagta atcgaaaaaa
    16561 tcaaagaatt gccaactgag catgtctata ttttagctac ttacactgct gtattgcagc
    16621 ttcgtaaaga actagctgct caaggattta tcaaaggagg aatgaaccgt ggctaattat
    16681 gaactcaata tcgcccacct ttatggaaat ttgatgaaca catatggtga taacggaaac
    16741 cttttgatgt tgaaatatat agcagaaaaa atgggggttt cttgccatac ggagatcgtt
    16801 agtatacatg aaccttttga tgctgataaa tatgacttgg ttttctttgg cggtggtcaa
    16861 gactttgagc aattaatcat ttcaaaagac atccagaaaa agaaagattc tttgatcaac
    16921 tatatcgaaa atgatggcgt catgttagcc atttgtggtg gctaccaatt gctaggtcac
    16981 tactacatgg gagctaatgg agaaaagatc caaggaatca gtgcactgga tcactacacg
    17041 ttaagtcagg aaaataaccg gtttatcggt gatatcgtta tccataacga agaattcaat
    17101 gaaacttatt atggatttga aaaccacaat ggtcggactt tcttaggtga aggtgaacga
    17161 cctttgggga aagtggtaca aggtcaagga aataatggtg aagaccaatc tgaaggtgtc
    17221 atttaccgaa atgtcttcgg ttcttatttc catggaccta tccttgcaag aaatgagaac
    17281 ctggcagctc gtttgatccg cctagcttta gaacaacgtt acggtaaaga attggatctg
    17341 ccctatcttg aaggacaaac agcctagtta ttcgaaaaag gggaatgtga caaaaatttc
    17401 gtcacattcc cctttttcgt atcctatttt aagcgacttg cttggcataa cggttcacgt
    17461 tttgcttgat attcccgatt gttttcggat cattgattcc tgtgacttct tgaatcaatt
    17521 gttccaagtt ttcatggatc ttcatttcat gcaattgacg gctttgttca tcttcaggat
    17581 catagtaatt aaaaataatc cccattgttg ccgtcaaatg tgggcaagtc aagttatgtt
    17641 cttgtagttc tctcactgga cgtgtaaatc tttcttgata acctaactta cgtaatggtg
    17701 tccgagcgac acgtgtgatc gcatcagaga tatttttgtt ttggaagcga tgaatgattt
    17761 tttcaatata cgcatgatgc tgctctgcat caaaccccca tttggcaatc agcaaacttc
    17821 ctgtttcctg caaaactgat tttaattgtg ccacaactaa cgcatcttgc attgcttcat
    17881 caatggtctg gtaacctaac aaggcacctg tataagcgac agtcgcatga ccagtattga
    17941 cactaaatag tttgcgttcg atatatggtt ctagatcatc tacatataaa acccctttta
    18001 attggatttt tttatttttt ctttggctat cgtcaatgac ccattctttg aaaggttcca
    18061 cttggacgaa taaaggatct tcatggtgtt gtaatgggac gattcgatcg actgctgcat
    18121 ttggaaagcc gatatatttg tcagtaaatg atgtttccgg aagatattta tacacttctt
    18181 tttctagaaa tgctgaaccg ccaatcatat tttcacaggc aatgatatct aaagatgttg
    18241 tgcttccttc cttttctctt tcttggatac cttttgcaat caactcagca ataaaaggta
    18301 agatattcgg gccaatggcg gtagtaacta aatctgcttc agcgatttct tggattactt
    18361 tttctggatt tgtttgattg ttgattcctt tgacattttt tacagtgatt ttttcttttg
    18421 attcatctgc taattcgatt tcgtattctt ttctttcatt taaagcatca atgatcgtgt
    18481 gattcacatc tacgaaagta atctcaaatc cattagctgc caaaacctct ccaataaatc
    18541 ctcgaccgat atttcctgca ccaaaatgta ctgctctcat ttttctctct ccttaagcaa
    18601 ttgctagatt ttccgttact tcttctttgc tgagcgcgtc tgctagctgg acaacattgt
    18661 ccatatcact gcaataaagt gcgatttgct gtacaagttg caaatgctca tctccgactc
    18721 ctgcaatacc gaacaaaaca gtcgcaatct tttcttcttc ttcggtacca aaatctacac
    18781 catctggtac ttgaacgaca cagatgccgg attttttcac atattttttt gcttcatctg
    18841 ttccatgagg aatcgcgata aaattcccca tataaacaga taacattttg tctctatcta
    18901 ccatggcatc gatatattcc tcatcaacgc accctgcttc tacaagcatc tgaccgcaat
    18961 agcggatcgc ttcttctttg gttttaaaag atcgattgag ctcgatcatg tcgaatggca
    19021 aactttgcat gctgttttcc tcccttaagc tgtctgtttc aaatattgtg caatcgattc
    19081 gtatttttgc ggattgacca tgctgttgac tgataaatga atggattgag gtgcagccat
    19141 ctgtactttt tccgctaaac tttctttggc tacgatcagt gaatcaggtt catcttttaa
    19201 atcttggata ttgacttttc ttaacgggat agttaaatgg ttcttgtcta atatatcttt
    19261 gagcacggtc atccccattg ttgctgatcc ggaacgatca tcgtggacaa gtagaatctc
    19321 tttgatttgt ttgatgtttg ctgaaagaaa ctcttctgct ggctcattct caatttcaga
    19381 aactgatgtt ttctgcttgc tgcttaattc ttgaatgatt tcatcatatc gaggtgaatt
    19441 caaaaagttt tctactgaga caaaacgcgc gctcggtgct ttttgttttg ctctttcttg
    19501 tagttcgacc tgagtaacga tcaatgtatt cgagtcatct gtcagttggc tgattgcttg
    19561 attggtcacc gtttgaggca agtctgcttc ctgtactttt tttctcaaga tcgaagcgcc
    19621 catcgcactt gatcccattc ctgcatcaca agcaaaaatg attttttgta cgccagagaa
    19681 tgtagctcct tgaggaatac cttgggcacc tttggaagct tgcttagctg cctttgtctc
    19741 ttgaacagat ttttcgaagt cttcatttgt atctttcgca tccgctttca aaattactgc
    19801 agaaatacca aacgaaacag ctgcggcaac aatcacgccc aaaataacag gaaggtaaga
    19861 attgaacggt gtcatcgcta gaatcgcaat gatcgaccct ggagaagctg gcgctcttag
    19921 tccagcatcc atcaattgga aaatgaaact tcctgacatt cctccgccta taactgataa
    19981 gaataataac ggtttcatca taacgtaagg aaaatagatt tcatggatcc cgcctaaaaa
    20041 gtgaatgatc atggctcctg gtgctgaaga ttttgctgct cctttgccaa acaaagtaaa
    20101 agcaagtaat acgccaagtc ctggaccagg atttgcttca agcaagaata gaatagattt
    20161 tccagcctct gctacttgtt cagttcccaa tggtgtcaaa atcccatgat tgattgcatt
    20221 attcaaaaat aagatttttg ctggttcaat aaaaatactt gtcaatggaa tcaaatgggc
    20281 agataaaatg atctctacac cttttgccat tgcttgagtc aatgcatcga caactggacc
    20341 aatcgcagaa aaacctaata acgctaatcc aaaaccgata agacctgctg aaaaattatt
    20401 gactagcatt tcaaaaccgg atttgatccg tttttggaat agctgatcaa atttcttgat
    20461 ggcatatcca ccaagaggtc ccatgatcat cgcgcctagc atcattggaa tatctgttcc
    20521 gacaatcaca cccatcgttg caattgcccc aacaactgat ccgcggtcac cagcaattac
    20581 ttttccgcca gtataaccga tcaataaagg aatcagataa gttagcattg ggcttaccat
    20641 ggtagcgaaa gcctcatttg gtaaataacc atctgggata aatagagccg ttaatacacc
    20701 ccaggcaatc agtgctccaa tatttggcat gaccatgctt gataaagtac tgcctaattt
    20761 ttgtaccctc gcttttagcg aaatgttgtt tttgggaatc gtccgttcca ttttgtctct
    20821 ccctctctcc ttttgatact ctaattgtat ccgcttacta aaaagagaaa tagtcgtact
    20881 ttggcacgaa actttgtgcc aaaggtttga gtatgtcagc aaaataaaaa gatttcggta
    20941 tcagaaaact tcaattttaa ataaaactgt tattttaatt tttttataat atgcaaacaa
    21001 taacttttgt tagcatttaa tttagatgtt ttccattcta ttatgttatt aaattttata
    21061 ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt ggtaaaaaac
    21121 gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct ttgattgccc
    21181 cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg ccaacaactt
    21241 atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat cgccggtctt
    21301 tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt gattatttga
    21361 ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc caacgattga
    21421 agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg aaacaacatg
    21481 tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat gaaccgttga
    21541 acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa tggcagaaag
    21601 aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg atttgttcta
    21661 aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg gaagagattt
    21721 tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat ttgaacgcaa
    21781 tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg tgctctttgc
    21841 attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac tgaatcataa
    21901 ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg agaaagaatt
    21961 tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg caaaaaacac
    22021 agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata tactggatcg
    22081 actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac gtgtcgagtt
    22141 gctccgttat atgaaagaac atcagatcca aagatattct atttttgact tgccagcgca
    22201 tctctcttta gcaaattact acgaaaatat tttttcagga atgatttcta gttttttgat
    22261 tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa gaaagcaggt
    22321 aatctctcta gtcaatctat taccggatag tatggtaaag aaacatagca tccgttttac
    22381 catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg tctctatact
    22441 agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga tcattggtca
    22501 ggagatacga atcctgccga tgtatgagta tcttttccag agctttctgt tcctgttgtt
    22561 gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg aacattctct
    22621 agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct tgttttcttc
    22681 gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg attttcaaaa
    22741 tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta ctttcatgaa
    22801 tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta tgggcactat
    22861 ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa aaaccttgtc
    22921 accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa agcttgcatc
    22981 cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt ggttgaagtt
    23041 attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg tccatgttca
    23101 aaaatcattt ggacttcttc aatggtttcc gcatcactga gcgccattac ttggtcgatg
    23161 tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt ttttaaagcc
    23221 actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc cacaccatca
    23281 ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc agtcccatgt
    23341 ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc gatcattgat
    23401 tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat ctcggtcaac
    23461 gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg tagctgtatc
    23521 atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca actgataaac
    23581 gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga tgattgctcc
    23641 actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg ccagcataac
    23701 taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca acggatgttt
    23761 caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat ggaataaccc
    23821 catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca gccggcgatg
    23881 aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc tgcgaatcgt
    23941 ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga gtaattgatt
    24001 gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc actcgttatt
    24061 gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag cttcttttag
    24121 ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc caccgtatcc
    24181 tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga ttttttttaa
    24241 atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt tcaatcgtag
    24301 ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac tgatattgct
    24361 ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag cgatctcttc
    24421 ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg cattcgcaac
    24481 ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct cttcttgtaa
    24541 aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca aagtttcatc
    24601 ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt ttactaaata
    24661 agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt tcgtgaagtc
    24721 atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt ttgtctcaga
    24781 agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg ccagactatt
    24841 atatgtctca tttgtcagta aatgattttt cgtcacacgc tccatcgtca atgtcagcat
    24901 caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga tcaattcttc
    24961 cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa aaaaataatt
    25021 cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt ctaattctaa
    25081 tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt atccctggtt
    25141 ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga tatctgcata
    25201 aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca acggctctgt
    25261 cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata agtcattact
    25321 tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct caatgacaaa
    25381 tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga cttcaagatt
    25441 atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca ttttttggat
    25501 agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc tgggcgataa
    25561 atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct acccaatgtt
    25621 caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct agttctcttt
    25681 gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct tttgagataa
    25741 caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc atagtttgtg
    25801 agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacgtt ttttgttctg
    25861 tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct tcttttattc
    25921 gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga ctttgaggta
    25981 ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga tttacttggt
    26041 tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct gctgctaatt
    26101 tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg caagcaaaaa
    26161 tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc gttttgttct
    26221 ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc acaaatgtac
    26281 tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg gcaaaaatca
    26341 cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat tgaaatatca
    26401 acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc agcaagacaa
    26461 atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct ccactcgcat
    26521 ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct aaaccaggtc
    26581 caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag aattcgattc
    26641 ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact tttagaggtt
    26701 ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac acaacggagg
    26761 cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca attatacaaa
    26821 taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat ccagccttga
    26881 atttcacatg gaatctagaa acaaattgtt catatacgat gccggcaagc ggcccgataa
    26941 tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa gaagcaattg
    27001 caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa ctgatcaaga
    27061 tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg tgcaaatcgc
    27121 cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga cttagatttg
    27181 gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata gacacgcgac
    27241 tgctgttgtt tgctttccat ttgtattcca tctttttcac cagacttcta tttattgatg
    27301 gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt cagctatctg
    27361 ggaactattt gcctggttca gacgcaatga ttgctagttc accttggacc gcccactcag
    27421 aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt tttccgtcaa
    27481 cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct tgttttttga
    27541 aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt tctaaataag
    27601 tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt gttttcgcag
    27661 ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt ccttgcgcat
    27721 cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt gtttctaaaa
    27781 tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag aaatcacctt
    27841 ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc atctcagcta
    27901 gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt tcagcatcga
    27961 tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca taggcatcat
    28021 ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc aataatggaa
    28081 acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt tcatctaatc
    28141 ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc gcactgatcg
    28201 cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa cgtaaacgac
    28261 ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc tgcataattc
    28321 aaactcctct catttttcat agctattata gcattatttc ggtaataccg ctttctcttt
    28381 caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat gatcgacagg
    28441 atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa tcaagaaaaa
    28501 cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat ccttttttaa
    28561 gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca gtaaagaatc
    28621 aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca aatacatttg
    28681 gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg catgttcagc
    28741 caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg aaggcagttg
    28801 ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca tcattcctaa
    28861 tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa tttcctctag
    28921 catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc ctagttgctc
    28981 acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct tatctctgtt
    29041 tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag tgattgtctg
    29101 attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct ttccatcttc
    29161 taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg ctgcatcaaa
    29221 caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc cggctgtaaa
    29281 tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa atccttcttc
    29341 catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct gcttttctat
    29401 cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt ttttacacaa
    29461 aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct ttctatctct
    29521 agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat cgttaagaaa
    29581 gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc tcactgacga
    29641 accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt ttgtctaaga
    29701 agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt tcagtatcga
    29761 tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca attgcgcctc
    29821 cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg atgccctctt
    29881 cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct tttgttaagt
    29941 tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta ctgaattttt
    30001 cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct ctttggtcgt
    30061 tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg agcaaaatcc
    30121 ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta cggatacgac
    30181 aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg ttcagctttt
    30241 tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac atttcacttc
    30301 cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa tcaaagccaa
    30361 caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg gctacacttt
    30421 ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc ctctacgtca
    30481 agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt ttgttaaaaa
    30541 aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa aaactcttat
    30601 aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata
    30661 gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    30721 tttaaggaag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa
    30781 aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
    30841 ccgtttacca agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa
    30901 aaaacagctt ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa
    30961 caccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa
    31021 cggaaaaata gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt
    31081 gaacaccgtt tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa
    31141 aataataaat ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga
    31201 tctgcataga atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa
    31261 ccatgattca tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca
    31321 acgaaacttg tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt
    31381 cgtgtatcta cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat
    31441 gcccgcgcaa ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt
    31501 aatacagtgt tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta
    31561 gcagcctcaa tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag
    31621 atacttcctg taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca
    31681 tcacgtgaaa gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg
    31741 tagaaccgtt caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata
    31801 gcgaccatct caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt
    31861 gtgtagccga attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga
    31921 aggccagatt tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct
    31981 gaagcaactg ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag
    32041 aactgtgttg tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca
    32101 acttttactc cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc
    32161 aacggcatga tgattgccga aataaattga gctttccaac cagcggcata cagattctga
    32221 ttttcttttt caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta
    32281 tgaccaccat atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct
    32341 gcgaaaaaca cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc
    32401 atcaataacg cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca
    32461 atcaatgtga ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg
    32521 ttatccatat cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag
    32581 gaaatcggta cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt
    32641 tgggaaacac gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac
    32701 atcacgatta ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt
    32761 acttgttggc cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg
    32821 gttgctttcc ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt
    32881 acaaatacag cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc
    32941 gttccccaga aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca
    33001 tgtcctggac cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt
    33061 cctcctctct tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt
    33121 cttcatgtgt tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat
    33181 ccattactgt acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt
    33241 tcaatgcttg gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa
    33301 acacatagat atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc
    33361 caccagagaa gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg
    33421 aaacaaaatc tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat
    33481 ccggcttacc atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag
    33541 gaacaaagcc cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat
    33601 ttatttggat cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg
    33661 ttttacctga accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat
    33721 caatatcttc caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg
    33781 ccaatgttgc gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac
    33841 cgattttttc atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca
    33901 tgataaaaat agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa
    33961 atgcaatcag attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag
    34021 tgattgctac gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga
    34081 tcgtattaac tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt
    34141 cttcaaagcg tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa
    34201 ataccaggtt taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt
    34261 acatgattcc gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca
    34321 actgatgatc tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga
    34381 tcatcatacg taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc
    34441 ttgtgatcaa agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt
    34501 ttttatagac atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa
    34561 atgtatttgc gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg
    34621 tatgccaaat ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa
    34681 gtgtcggcaa atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg
    34741 ctgaagtcaa ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat
    34801 atactccgtt tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg
    34861 agtaatcttc agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat
    34921 tcatcactaa ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc
    34981 gatcgatcaa ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag
    35041 gcgttttttg gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca
    35101 taaacaagtt acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag
    35161 aaaaatagga ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca
    35221 tatctttaga gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat
    35281 cttcaaaata ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa
    35341 tcgaagcttc ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt
    35401 tttgtttttc tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt
    35461 tcaagatgac acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca
    35521 agacatttgc atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat
    35581 ccttccattt gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg
    35641 agataaatgg cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac
    35701 agcatcgttt tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg
    35761 ctttctatcc ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt
    35821 tattatattc taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct
    35881 aatggttttc gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct
    35941 tgaatattgc ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt
    36001 tataccctgt ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct
    36061 tttctttttc gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag
    36121 gagaaggtaa gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag
    36181 aatcgcgcac agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag
    36241 atgatgaagt gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat
    36301 tattatcagg ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc
    36361 aacaacaaaa aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa
    36421 tcgttatcgc aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg
    36481 ataccgtcaa tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag
    36541 tgacaggtaa aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact
    36601 ttgcctctca atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc
    36661 tattcatcga aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat
    36721 tagataaagc tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag
    36781 agacagagga aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc
    36841 tttttgttat tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt
    36901 gtaccattca aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa
    36961 gatcacaccg atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac
    37021 tggatggatt gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt
    37081 ctgctctact gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc
    37141 taaaaagata gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa
    37201 acccgcaata atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat
    37261 aaacatgatt gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg
    37321 actattcatt tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt
    37381 gacaaaataa ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat
    37441 cgtcagcaga gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac
    37501 tgtactctta gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc
    37561 tgtttccaac tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt
    37621 attatcaatc gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg
    37681 aatcttaggg atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag
    37741 cccaatgata ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt
    37801 catcaagtaa tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg
    37861 ccagattggt gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa
    37921 caatacaaca agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga
    37981 taccggtctg ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt
    38041 tggcttccca aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt
    38101 tgtccttctt ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc
    38161 ccattggcca tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt
    38221 ccgcctgctt tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt
    38281 cgtaaggcag ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt
    38341 gcttctgtcc caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct
    38401 ttcaaaaaca gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa
    38461 taaaaacgaa aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata
    38521 attttaggaa cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca
    38581 gaaaaagaag aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta
    38641 gcaaaaagta aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc
    38701 ggtgcgtacg ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg
    38761 tgttatgtag cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt
    38821 gaagtaaatg tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc
    38881 catcaagaag cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat
    38941 ctgactcctg accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat
    39001 gatgtgtccg aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca
    39061 ggccctcgcc atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta
    39121 gacagcacag tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag
    39181 aaaatatcga ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt
    39241 tcaaatgatg gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc
    39301 gcagtttttg aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat
    39361 tttccccgcg atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac
    39421 accgataatc tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa
    39481 aaagatgtct atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag
    39541 aatgaaatga atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc
    39601 tttgatggga aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc
    39661 gatttacaga actactttga acaatatgta cagaatgtag aaaactaatc agctttccat
    39721 actatctgaa aagaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca
    39781 aataaaaaac cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag
    39841 taagtacaag gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc
    39901 tatcataaag gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga
    39961 tttcttttat tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc
    40021 tgtcgcttgg tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc
    40081 aagatcgata ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc
    40141 aaaattttta tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag
    40201 cattgcccga tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga
    40261 tttgtccagt tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg
    40321 atgcattcct tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata
    40381 atagccaata ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat
    40441 tctacttgtt tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag
    40501 ctcgccattc ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga
    40561 caatgtcata ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt
    40621 gcgcttcttt ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt
    40681 cctgttctaa tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca
    40741 ttagattcgc tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta
    40801 attctccttt actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt
    40861 ttgcttggat cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag
    40921 tccttctaga aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg
    40981 aaatatcttg cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag
    41041 gatagtctag attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat
    41101 gaatgaactg aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact
    41161 catcagaaat atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga
    41221 tgaactggtc aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt
    41281 atggatggat cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa
    41341 gctgatccat ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg
    41401 ccatagaaaa accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt
    41461 gagaaagcat attgccacta tatgtttgat cagctacttc ataataatct gccaatggct
    41521 caccagatac ttctacgccg tttaatcgac tatgatctga gaaactttca ctcatcacga
    41581 acacgatgtt cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt
    41641 atttttcagt gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt
    41701 ctaccttcag attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct
    41761 ggctgtaagg tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat
    41821 tgttgaaatt actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga
    41881 tcaattggaa tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta
    41941 tcacggttcc tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca
    42001 gcaatccgat ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac
    42061 tcattttcat ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac
    42121 ctaaagggac agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac
    42181 aggctaagaa gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg
    42241 atagatcatt ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac
    42301 tgatgattgc tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat
    42361 atcttttcaa atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt
    42421 tagttgtacg aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc
    42481 tctttcttta tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt
    42541 tttctagctg ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca
    42601 atatgtagac atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct
    42661 cattcagcag agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga
    42721 atcgaaacgg cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg
    42781 taccgaacaa aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag
    42841 cttcctgagt cgatccccct agatgaggca acataagtat acggtcattt tctaagaact
    42901 cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa
    42961 cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca
    43021 ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga
    43081 ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca
    43141 actgggcatc gcggatagaa taacccaaga catccatccc taagctatag caacttagcg
    43201 ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc
    43261 gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg
    43321 tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa
    43381 caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt
    43441 cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca
    43501 aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg
    43561 gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa
    43621 tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa
    43681 atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag
    43741 agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt
    43801 ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat
    43861 tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt
    43921 tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact
    43981 caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat
    44041 gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt
    44101 tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta
    44161 cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt
    44221 ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag
    44281 gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg
    44341 attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg
    44401 tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc
    44461 cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt
    44521 gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta
    44581 agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct
    44641 tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat
    44701 cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat
    44761 caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt
    44821 ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg
    44881 caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga
    44941 cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag
    45001 cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat
    45061 attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt
    45121 ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga
    45181 cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg
    45241 gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta
    45301 cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt
    45361 tttctccaat attcaacata attagccact ccttttattt gttttgtccg taatacgcat
    45421 tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa
    45481 atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt
    45541 tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta
    45601 cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc
    45661 ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta
    45721 ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt
    45781 gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg
    45841 aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga
    45901 tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc
    45961 cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg
    46021 tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca
    46081 gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca
    46141 atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct
    46201 gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct
    46261 aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca
    46321 ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc
    46381 tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca
    46441 ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg
    46501 atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt
    46561 ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg
    46621 caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt
    46681 atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc
    46741 gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg
    46801 tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa
    46861 gtaccagccg aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt
    46921 cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg
    46981 atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta
    47041 ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata
    47101 tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca
    47161 tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt
    47221 gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg atacaaatga
    47281 gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct
    47341 tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca
    47401 aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc
    47461 tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc
    47521 caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa
    47581 ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat
    47641 ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt
    47701 acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt
    47761 agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca
    47821 agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac
    47881 aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa
    47941 aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga
    48001 tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca
    48061 ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc
    48121 ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct
    48181 gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat
    48241 tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt
    48301 cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat
    48361 caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg
    48421 ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt
    48481 ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc
    48541 ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa
    48601 ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact
    48661 ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag
    48721 caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact
    48781 gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct
    48841 ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata
    48901 ggcggtagcg tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat
    48961 gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct
    49021 tgcacatggg ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc
    49081 gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg
    49141 ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc
    49201 atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct
    49261 ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat
    49321 cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta
    49381 gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga
    49441 cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag
    49501 ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag
    49561 aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt
    49621 acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac
    49681 cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc
    49741 cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga
    49801 tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg
    49861 atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct
    49921 cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca
    49981 cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg
    50041 aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc
    50101 acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc
    50161 atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta
    50221 cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac
    50281 tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct
    50341 cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg
    50401 gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca
    50461 tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg
    50521 attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag
    50581 ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca
    50641 atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa
    50701 cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg
    50761 aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc
    50821 gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata
    50881 gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa
    50941 ttgcttattt ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt
    51001 taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt
    51061 tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc
    51121 atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt
    51181 cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat
    51241 ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc
    51301 gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt
    51361 cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg
    51421 tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga tataatggac
    51481 ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag
    51541 gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta
    51601 gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc
    51661 tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac
    51721 attttcttta ttctaattac attccaagag tatttttcta tccctacatt taattcgtca
    51781 tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta
    51841 tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg
    51901 aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc
    51961 agctgattgc cgttatctac gatcacactg tccacataag gaacattttt tatcttttca
    52021 caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat
    52081 aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt
    52141 attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt
    52201 cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac
    52261 caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca
    52321 ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa
    52381 tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat
    52441 gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta
    52501 tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata
    52561 cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca
    52621 attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct accatattca
    52681 taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat
    52741 ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct
    52801 tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa
    52861 ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt
    52921 ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct
    52981 ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga
    53041 atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca
    53101 tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca
    53161 acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt
    53221 actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata
    53281 gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg
    53341 agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat
    53401 actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc
    53461 acatatatcc aggataaaag aaacatggaa taaataagta aaactacata tccactaatt
    53521 gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt
    53581 aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa
    53641 atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta
    53701 gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg
    53761 taatcagaaa ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc
    53821 actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa
    53881 ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa
    53941 tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg
    54001 tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg
    54061 ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta
    54121 atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca
    54181 tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa
    54241 gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca
    54301 tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa
    54361 atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa
    54421 tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat
    54481 ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt
    54541 taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca
    54601 tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg
    54661 taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt
    54721 gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac
    54781 agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga
    54841 acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac
    54901 acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga
    54961 ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca
    55021 tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa
    55081 ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat
    55141 tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca
    55201 agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat
    55261 gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa
    55321 ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa
    55381 ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt
    55441 gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg
    55501 acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca
    55561 ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata
    55621 aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt
    55681 ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa
    55741 cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct
    55801 gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg
    55861 atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag
    55921 tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct
    55981 actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt
    56041 gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc
    56101 ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt
    56161 ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc
    56221 ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa
    56281 atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta
    56341 aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca
    56401 tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat
    56461 acatttttac tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta
    56521 ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac
    56581 atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata
    56641 gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt
    56701 tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca
    56761 ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg
    56821 atatccattt caattttgat gtttttatca tttgttttat taaattcatt aacgatttct
    56881 tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta
    56941 tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct
    57001 accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga
    57061 taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc
    57121 ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt
    57181 tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc
    57241 agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag
    57301 ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt
    57361 cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc
    57421 ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat
    57481 tggtgcaata acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt
    57541 caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc
    57601 tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata
    57661 ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt
    57721 gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc
    57781 aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc
    57841 tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg
    57901 ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt
    57961 agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg
    58021 accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt
    58081 cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt
    58141 acgagctgca tctactccac gagtacccag attgacagcc atattgattt cggcccccac
    58201 atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca
    58261 agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa
    58321 tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa
    58381 gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat
    58441 cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac
    58501 tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta
    58561 atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt
    58621 acttattttt tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt
    58681 aatataactt ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat
    58741 atatgcaact acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa
    58801 caaggatcaa gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa
    58861 aagaacttgg agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa
    58921 acatcatcaa aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa
    58981 tcgatatcag ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac
    59041 ctattggtca ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg
    59101 attttatcgg gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc
    59161 gcattctctg gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag
    59221 aggatacatt ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa
    59281 atgacaattg gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga
    59341 cgagccctgg agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata
    59401 atcttaatca atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga
    59461 atggagaacc cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc
    59521 atttgcgaat cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta
    59581 aaggattcgg taattacgat caggatatca aattaaaagt ttactacagc tgatattttt
    59641 cttttctatt ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt
    59701 tattgttatt tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc
    59761 ttgttccacc atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga
    59821 taatcatcaa tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc
    59881 ccatcttcaa gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct
    59941 tgaagcaatt gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca
    60001 ccatcaccaa atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat
    60061 acggctcctg ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg
    60121 atatttcctt tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt
    60181 gcatcaacag ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga
    60241 gcagcttttg catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat
    60301 tgatagtttt catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg
    60361 cctgctaaag tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga
    60421 tattttttca aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca
    60481 attgttggtt tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct
    60541 cctatgcttc tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt
    60601 tatggttcca gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt
    60661 tatactcttc ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg
    60721 caggtaattc gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg
    60781 taaagtctcg aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt
    60841 cttcttctcg agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa
    60901 aatttcgagt gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc
    60961 ttgataactt gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga
    61021 atactgcacc aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata
    61081 catcaatttt agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg
    61141 aagcatagat ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag
    61201 atatataggc catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta
    61261 attctttcgc cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga
    61321 ctaagaacac gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc
    61381 cataagattg gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc
    61441 cagcacgtac attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga
    61501 ctgctgcatc cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga
    61561 tgacagattt gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca
    61621 acagtgctgt ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt
    61681 catttgcaaa aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat
    61741 tttgtaaata ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat
    61801 cggaatcatt gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa
    61861 ccgcgtatcc gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat
    61921 cagctcgacc taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca
    61981 gcgtcaaact gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt
    62041 tcatttccta cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct
    62101 gaaaaagcac atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta
    62161 tcctttctta ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc
    62221 gagacacgaa gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc
    62281 tactacaatc gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac
    62341 aacataatca atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta
    62401 tccccaaaga aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg
    62461 acaagatata ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga
    62521 tacaaagtaa gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag
    62581 tctcgccttg ttttttattg tatacattag atatggagca ggttaccata aagatgatta
    62641 ttagtcgtta ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat
    62701 ttggacgttt gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt
    62761 tgacatcttc tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg
    62821 tgattttttt cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata
    62881 gcagttgtgt tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa
    62941 agaattccac tactttagga taaaatgtct taatatttaa attactattc acaggtgttt
    63001 cgattagctt catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta
    63061 tgttaccatc tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg
    63121 atttaccttt tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt
    63181 cttgaccatc tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga
    63241 gtacacaata aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta
    63301 tgatgagatt ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg
    63361 caaaaaagaa gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt
    63421 ggatgatatc caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa
    63481 gcatcagcaa atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca
    63541 acagacactt gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg
    63601 gtttaaacaa caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa
    63661 atacggaaaa aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac
    63721 gtatgaagaa atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa
    63781 agaaccagaa gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc
    63841 ttacagctgg cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc
    63901 cgatgaacgg ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt
    63961 aaatgaaatt atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt
    64021 aaaatgacct aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt
    64081 gtctgctcca ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata
    64141 aacgtataag gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt
    64201 tctccgcaag cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc
    64261 aaattatcgc gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct
    64321 gctgcaacca tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag
    64381 cctgcttgtt ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa
    64441 gatggaaaaa gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca
    64501 cttcgcccca acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct
    64561 tctgtaattg attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct
    64621 tcacaaaaaa ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca
    64681 cgagacgtaa tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc
    64741 tctttttctt tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct
    64801 gcttcaatga tcccactgat caactgatca aaatacggat gattggtata aggcagaatc
    64861 actccaatgc ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa
    64921 tcgacttctt tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt
    64981 ccattgatta cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga
    65041 atgtttgcca ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca
    65101 cagctggacc gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct
    65161 gcccgcccca atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta
    65221 ctatattcct attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga
    65281 caaacgcata aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg
    65341 caagccctcc ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt
    65401 tgttttgcca aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa
    65461 ttgaatacat tataatgata ctgtaaatat atctttatta ttagtgaatg
//
LOCUS       DAJSVP010000008        63829 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSVP010000008 DAJSVP010000000
VERSION     DAJSVP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.008"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            242..1456
                     /locus_tag="ON449_000480"
     CDS             242..1456
                     /locus_tag="ON449_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3933100.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1487..2914
                     /locus_tag="ON449_000481"
     CDS             1487..2914
                     /locus_tag="ON449_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933101.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2937..3863
                     /gene="glsA"
                     /locus_tag="ON449_000482"
     CDS             2937..3863
                     /gene="glsA"
                     /locus_tag="ON449_000482"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3933102.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4071..5531
                     /locus_tag="ON449_000483"
     CDS             4071..5531
                     /locus_tag="ON449_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933103.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5752..7515
                     /locus_tag="ON449_000484"
     CDS             5752..7515
                     /locus_tag="ON449_000484"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3933104.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7599..8057
                     /locus_tag="ON449_000485"
     CDS             7599..8057
                     /locus_tag="ON449_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3933105.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8166..8849
                     /locus_tag="ON449_000486"
     CDS             8166..8849
                     /locus_tag="ON449_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3933106.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8996..9610
                     /locus_tag="ON449_000487"
     CDS             8996..9610
                     /locus_tag="ON449_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3933107.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9626..9832
                     /locus_tag="ON449_000488"
     CDS             9626..9832
                     /locus_tag="ON449_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933108.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10371..10928
                     /locus_tag="ON449_000489"
                     /pseudo
     CDS             10371..10928
                     /locus_tag="ON449_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10986..13025
                     /locus_tag="ON449_000490"
     CDS             10986..13025
                     /locus_tag="ON449_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3933109.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13134..14285)
                     /locus_tag="ON449_000491"
     CDS             complement(13134..14285)
                     /locus_tag="ON449_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3933110.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14439..14969)
                     /locus_tag="ON449_000492"
     CDS             complement(14439..14969)
                     /locus_tag="ON449_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3933111.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15068..15418)
                     /locus_tag="ON449_000493"
     CDS             complement(15068..15418)
                     /locus_tag="ON449_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3933112.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15575..17104)
                     /gene="ahpF"
                     /locus_tag="ON449_000494"
     CDS             complement(15575..17104)
                     /gene="ahpF"
                     /locus_tag="ON449_000494"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3933113.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17114..17677)
                     /gene="ahpC"
                     /locus_tag="ON449_000495"
     CDS             complement(17114..17677)
                     /gene="ahpC"
                     /locus_tag="ON449_000495"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3933114.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17821..18483)
                     /locus_tag="ON449_000496"
     CDS             complement(17821..18483)
                     /locus_tag="ON449_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3933115.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18667..20313
                     /locus_tag="ON449_000497"
     CDS             18667..20313
                     /locus_tag="ON449_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3933116.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20304..21386
                     /gene="uxuA"
                     /locus_tag="ON449_000498"
     CDS             20304..21386
                     /gene="uxuA"
                     /locus_tag="ON449_000498"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3933117.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21494..23047)
                     /locus_tag="ON449_000499"
                     /pseudo
     CDS             complement(21494..23047)
                     /locus_tag="ON449_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23195..24336
                     /locus_tag="ON449_000500"
                     /pseudo
     CDS             23195..24336
                     /locus_tag="ON449_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24462..25541)
                     /locus_tag="ON449_000501"
     CDS             complement(24462..25541)
                     /locus_tag="ON449_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3933118.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25650..26090)
                     /locus_tag="ON449_000502"
     CDS             complement(25650..26090)
                     /locus_tag="ON449_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3933119.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26147..26557)
                     /locus_tag="ON449_000503"
     CDS             complement(26147..26557)
                     /locus_tag="ON449_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3933120.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26738..27172)
                     /locus_tag="ON449_000504"
     CDS             complement(26738..27172)
                     /locus_tag="ON449_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3933121.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27272..28120)
                     /locus_tag="ON449_000505"
     CDS             complement(27272..28120)
                     /locus_tag="ON449_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3933122.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28509..30275
                     /locus_tag="ON449_000506"
     CDS             28509..30275
                     /locus_tag="ON449_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3933123.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30377..31636
                     /locus_tag="ON449_000507"
     CDS             30377..31636
                     /locus_tag="ON449_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3933124.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31783..33084
                     /locus_tag="ON449_000508"
     CDS             31783..33084
                     /locus_tag="ON449_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933125.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33087..33938
                     /locus_tag="ON449_000509"
     CDS             33087..33938
                     /locus_tag="ON449_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933126.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33951..34766
                     /locus_tag="ON449_000510"
     CDS             33951..34766
                     /locus_tag="ON449_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3933127.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34917..36083
                     /locus_tag="ON449_000511"
     CDS             34917..36083
                     /locus_tag="ON449_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3933128.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36313..36960
                     /locus_tag="ON449_000512"
     CDS             36313..36960
                     /locus_tag="ON449_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3933129.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37044..37988
                     /locus_tag="ON449_000513"
     CDS             37044..37988
                     /locus_tag="ON449_000513"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3933130.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38123..39574
                     /locus_tag="ON449_000514"
     CDS             38123..39574
                     /locus_tag="ON449_000514"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3933131.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39595..40431
                     /locus_tag="ON449_000515"
     CDS             39595..40431
                     /locus_tag="ON449_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3933132.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40431..41090
                     /locus_tag="ON449_000516"
     CDS             40431..41090
                     /locus_tag="ON449_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3933133.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41216..41905
                     /locus_tag="ON449_000517"
     CDS             41216..41905
                     /locus_tag="ON449_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933134.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41911..42867
                     /locus_tag="ON449_000518"
     CDS             41911..42867
                     /locus_tag="ON449_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3933135.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42911..43423
                     /locus_tag="ON449_000519"
     CDS             42911..43423
                     /locus_tag="ON449_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933136.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43679..44227)
                     /locus_tag="ON449_000520"
     CDS             complement(43679..44227)
                     /locus_tag="ON449_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933137.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44316..45497)
                     /locus_tag="ON449_000521"
     CDS             complement(44316..45497)
                     /locus_tag="ON449_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3933138.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45733..47241)
                     /locus_tag="ON449_000522"
     CDS             complement(45733..47241)
                     /locus_tag="ON449_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3933139.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47400..47591
                     /locus_tag="ON449_000523"
     CDS             47400..47591
                     /locus_tag="ON449_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933140.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47794..48711
                     /locus_tag="ON449_000524"
     CDS             47794..48711
                     /locus_tag="ON449_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3933141.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48704..49777
                     /locus_tag="ON449_000525"
     CDS             48704..49777
                     /locus_tag="ON449_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933142.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49925..50443
                     /locus_tag="ON449_000526"
     CDS             49925..50443
                     /locus_tag="ON449_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933143.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50424..50816
                     /locus_tag="ON449_000527"
     CDS             50424..50816
                     /locus_tag="ON449_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3933144.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50892..51827
                     /locus_tag="ON449_000528"
     CDS             50892..51827
                     /locus_tag="ON449_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3933145.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51960..52739
                     /locus_tag="ON449_000529"
     CDS             51960..52739
                     /locus_tag="ON449_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3933146.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52732..53049
                     /locus_tag="ON449_000530"
     CDS             52732..53049
                     /locus_tag="ON449_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3933147.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53293..54903
                     /locus_tag="ON449_000531"
     CDS             53293..54903
                     /locus_tag="ON449_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3933148.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55060..55908
                     /locus_tag="ON449_000532"
     CDS             55060..55908
                     /locus_tag="ON449_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3933149.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            56139..56618
                     /locus_tag="ON449_000533"
     CDS             56139..56618
                     /locus_tag="ON449_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933150.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            56651..58879
                     /locus_tag="ON449_000534"
     CDS             56651..58879
                     /locus_tag="ON449_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933151.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            59008..60651
                     /locus_tag="ON449_000535"
     CDS             59008..60651
                     /locus_tag="ON449_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3933152.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(60724..61269)
                     /locus_tag="ON449_000536"
     CDS             complement(60724..61269)
                     /locus_tag="ON449_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3933153.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(61292..61777)
                     /locus_tag="ON449_000537"
     CDS             complement(61292..61777)
                     /locus_tag="ON449_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3933154.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(61767..62579)
                     /locus_tag="ON449_000538"
     CDS             complement(61767..62579)
                     /locus_tag="ON449_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3933155.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(62718..>63829)
                     /locus_tag="ON449_000539"
     CDS             complement(62718..>63829)
                     /locus_tag="ON449_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3933156.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        21221 a        11429 c        12460 g        18719 t
ORIGIN      
        1 tttattcctt atgaaaaaat atctttaaaa ataaaaaaag acgaaaataa acggttttct
       61 ataaaaagaa gaccgtttat tttttgtctt tatttgtgtg aaaaacgaaa aagagcacaa
      121 cacatcaagc ttttattaat tgtctgtctt ttataaatgc aagtattaat tcaaaaaaac
      181 agaagaaaaa aacaactaca ccttctttta tactcgtaat cgtagaaagg aggcgaaaaa
      241 aatgaataca ttacctatca atacaggaga tacagcattc atgattttat gtacagcaat
      301 ggtttgcctg atgactcctg gtctagcttt tttttacggc ggattagcac ggaagaataa
      361 tatactaacg atcatgtctc aatcttttct ctccgttggg ataacaacga ttatgtgggt
      421 atttggtgga ttcggattgg cctttggacg ggatatcggc ggtgttattg gaaacccggc
      481 agatttcttt ttgatgagac atgttacaag cttccccaac atgtttcatg gtgcaacgat
      541 tccattttta atgttctttc tttaccaact gatgtttatc gtgattaccg ttcctttgat
      601 gacaggagct tttgcaggga gattgaattt aaaaggctat attatacttg ttattttttg
      661 gaatctgctg atttatttcc ctgtttgcca ctggatatgg ggtggcggat ttttggatca
      721 gatgggattt agagatttcg ctggtggagc agtcatccac acgacagctg gattcggctc
      781 tttagcatgt atcctcacac ttggtcaaag aaagattcct ttaggagaaa gaagtcagca
      841 tagcagtctt atggcagctg ctatcggtac aggactgctt tggtttggct ggttcggttt
      901 taattccggt ggtgcacttc gagcagatga acaagcagtc aatgcatttg gttcaacatt
      961 catcgctctt gcatttgcga tgattacttg gctgatcatt gcaaaagtaa aaggaaatgg
     1021 ctttgatttt gtcgacgtac taacaggatc cgttgctgga ttggcagcca ttacgccttg
     1081 tgccggttat gtagaagcaa aaagtgccat gctgattggg atcattgcgg gtatcgtctg
     1141 tcatgctgct gttgattttc gtaaaaagaa acaatgggat gatgcacttg atgtgtgggg
     1201 tgttcatggc atgggcggat tcacaggtac gattctgata gggatctttg catctaacag
     1261 ccatttacta acgaatccaa gcagctggta cttcttaggg attcaagtga ttggtgtcat
     1321 cattactgcc gtgtatgcct atgtgctgac aacagtgatt ttaaaatcgg cgatgcactt
     1381 tacaacgatc actactacaa aagaagaaca agaagaagga ttggactttt tcttctttgg
     1441 caacaaactc aattgaaaca aattcaaata gaaaagagga agattcatgc aacaaagcaa
     1501 aacgaaaatt cctgcaaaaa aattaacgtt ctttggcttt ttggctatga ccatctcaat
     1561 ggtcgtttca ttatatgaat atcccacatt cgcgacctca ggcttcagct tagtattttt
     1621 cttgttatta ggcggtttgc tatggtttat ccctgtggca ttgtgcgcag cagagatggc
     1681 aacggtgaaa ggatgggaaa aaggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     1741 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     1801 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     1861 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     1921 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     1981 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     2041 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     2101 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     2161 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     2221 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     2281 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     2341 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     2401 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     2461 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     2521 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     2581 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     2641 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     2701 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     2761 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     2821 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     2881 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     2941 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     3001 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     3061 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     3121 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     3181 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     3241 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     3301 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     3361 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     3421 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     3481 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     3541 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     3601 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     3661 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     3721 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     3781 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     3841 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     3901 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     3961 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     4021 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     4081 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     4141 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     4201 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     4261 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     4321 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     4381 gtacagaact ggctgatctt gaatttattt ctttgagcaa agtatataca atcaaaaaaa
     4441 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     4501 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     4561 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     4621 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga
     4681 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     4741 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     4801 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     4861 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     4921 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     4981 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     5041 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     5101 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     5161 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     5221 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     5281 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     5341 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     5401 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     5461 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     5521 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     5581 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     5641 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     5701 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     5761 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     5821 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     5881 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     5941 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     6001 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     6061 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     6121 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     6181 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     6241 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     6301 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     6361 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     6421 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
     6481 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
     6541 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
     6601 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
     6661 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
     6721 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
     6781 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
     6841 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
     6901 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
     6961 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
     7021 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
     7081 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
     7141 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
     7201 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
     7261 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
     7321 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
     7381 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
     7441 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
     7501 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
     7561 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
     7621 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
     7681 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
     7741 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
     7801 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
     7861 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
     7921 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
     7981 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
     8041 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
     8101 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
     8161 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
     8221 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
     8281 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
     8341 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
     8401 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
     8461 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
     8521 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
     8581 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
     8641 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
     8701 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
     8761 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
     8821 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
     8881 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
     8941 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
     9001 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
     9061 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
     9121 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
     9181 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
     9241 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
     9301 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
     9361 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
     9421 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
     9481 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
     9541 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
     9601 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
     9661 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
     9721 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
     9781 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
     9841 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
     9901 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
     9961 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    10021 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    10081 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    10141 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    10201 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    10261 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    10321 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ggtcgctcgg
    10381 attcacttct ggttgcacaa tacaaccaga aaacacaaca agcaaaactg atctctatca
    10441 tgagagattc atatgtcgat attccaggtt acggaatgaa taagataaat gcagcttact
    10501 cttatggggg agttgattta ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc
    10561 cctattatgc aagtatcaca tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg
    10621 gtggagtcaa gatcgatgca gaaaaatctc tagatttaga tggcgtatat ataaacaaag
    10681 gagagcaagt gatggatggt aatactttac tccagtatgc tcgatttcgg gaagacaaag
    10741 aaggagactt cgggaggatc agaaggcagc agcaagtgat caaagctatc tctcagcagc
    10801 tgaaagacgt aacttcaata ttcaagctac ccaaagcggt tgggaaatta cttggtagta
    10861 tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg tatggatttc ctcaaagaca
    10921 tatgttagaa taaacaggta taaatagtga aataaaggaa tgacaagcaa gagaaggagg
    10981 aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac aggcgcttat attgccggcg
    11041 cagtgatctt aatagcagct gcgggtggcg gttattttta ctaccagcac tatcaagaaa
    11101 cccaagcagt agaagctgga gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg
    11161 gagattataa caaagctgca gaaatgacat caaaaaaggc ggcaaataaa agtgcattat
    11221 ctgaaaaaga gatcttagat aaataccaga atatatacgg tgctgctgat gtcaaaggac
    11281 ttcagatatc gaatctaaaa gtagataaaa aagacgattc tacttacagt ttttcatata
    11341 aagcaaagat gaatacctca ttaggtgaat tgaaagatct ttcttataaa ggaacattag
    11401 acagaaatga tggtcaaacc acgatcaact ggcaacccaa cttggttttt ccagaaatgg
    11461 aaggaaatga caaagtaagc ctgaccacac aagaagcagc aagagggaat attatagatc
    11521 gaaatggtga accattagca acaacaggca aactaaaaca attaggagtc gttccaagta
    11581 aacttggaga tggaggcgaa aaaacagcca atatcaaagc gattgcttcc tccttcgact
    11641 taacagaaga tgctatcaat caggcgattt cacaaagctg ggtacaaccc gattactttg
    11701 tcccattgaa aatcattgat ggagcaacgc cagaacttcc agctggagct accattcaag
    11761 aagtagacgg cagatattat cctttgggtg aagcagctgc tcagctgatt ggttacgtgg
    11821 gagatatcac agcagaagat attgataaga atccagaatt aagcagtaat ggaaaaatcg
    11881 gtcgctcagg tttggagatg gcttttgata aggatcttcg tgggacaaca ggtgggaaat
    11941 taagcattac agatgcagac ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa
    12001 atggcaaaga tatcaaattg acaatcgatg cgaaggcaca aaaaatagct tttgacagtc
    12061 taggaggaaa agctgcatca actgttgcga caacgccaaa aaccggtgat cttcttgcgc
    12121 ttgctagctc tccaagctat gatccaaaca aaatgacaaa cgggatctca caagaagatt
    12181 acaaagctta tgaagaaaat cctgaacatc cattcatcag ccgatttgcg acaggttatg
    12241 ctcctggatc tacgtttaaa atgattacag cagcaatcgg tctcgacaac ggcactatcg
    12301 atccaaatga agtgttgacg atcaacgggc ttaaatggca aaaagacagt tcttggggat
    12361 cttttaaaac aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa actgctttga
    12421 tctattccga taatatatat gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc
    12481 gtacaggctt agataaattc atttttggtg aagaccttga tttgccaatt agtatgaatc
    12541 cagcacaaat ttctaatgaa gatagcttta actcagatat cttgctagct gatactggat
    12601 atggacaggg cgaacttcta attactccta tccagcaagc agcaatgtat tctgttttta
    12661 ccaacaatgg cacacttgtc tatcctaaat tgattgcaga taaagagaca aaagataaga
    12721 agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc
    12781 aagatgtaaa tggtacagca cattctcttt ctgctttagg gattccgttg gcagcgaaaa
    12841 ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt
    12901 ttgctttcaa tcttgataac caaggttata tgatggtcag catgttggaa aataaagaag
    12961 atgatgattc agcaaccaaa cgagcatcgg aactattgca atacctcaac caaaattatc
    13021 aataaaaaat tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc acagtccttt
    13081 tttcaaataa acggttttca cgatgtctct ttcacaactt ctttttgatt tactcacact
    13141 tcataaactt tttttgtaaa atatttgaga acaagtggag aaataagtgt acttaataaa
    13201 atcacgataa ctaaaggtga atagtactgt ggttcgatca attgactttg ctgtccaatt
    13261 tgtaaaatga tcaaagccat ttctccacgc gaaatcatac cagcaccgac catcaatgaa
    13321 ctatgttgag aaaaaccggc aatctgagca ccaaaataac ctccaagcaa tttggtcaaa
    13381 atcgctacaa gcgtcaatat agaaataaag agtagttgtt cactaaagtc agaaaaatca
    13441 acttctaatc ctacactgac aaaaaatact ggtataaata ctgcatatcc taaagcctct
    13501 acattgtggt atacttcttc tttaaccttt gtctgactga cagctatccc agcaaaaaaa
    13561 gcaccaatga cagagctaag cccaacaaga tccgctacgt aagacattcc taaacaaata
    13621 actagcgata cgatgataat agaagaattt gcgtacactt tttctgcaaa tgaaagtagg
    13681 aaaggcgcaa tccatttgac taataagaaa ataaaaacaa aataaattag ctgttcaatc
    13741 aaaacaagcg gtaaagaagc attgctagaa gcttcccccg ttagaaaaga taggctgaaa
    13801 cttagaacta aaacgactaa aatgtcatct accacagaag cacccaaaat tgttgatcct
    13861 tcttttgtat tcactacgtt tagttctttt aatacttcta ctgaaatact gacagaagtt
    13921 gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg aaacttggaa tgctttgcct
    13981 gtcaaccagc caaaaacaac cgggaagatg atcccaagca aagcaacgaa cataccaggt
    14041 cggaaatatt ttttcaaaag agataaatca ctttccagac cagctaaaaa cattaataag
    14101 atcacaccaa tctccgaaaa gtcatggact agaatatcag gtttgaccca attcaatcca
    14161 gcttggccta gcaaaacacc aacaagtaat tgtccaatca ctgctgggat acctaatctc
    14221 cgcgagatat gtgagccaat cgttgttgaa atcaatatca ggcataaaat accaataaat
    14281 tccattctca tcattccttt gtataaaata tagcccgaac ttagcataac atacattatt
    14341 gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc caaaagattt ttttggattg
    14401 ttggttttta tacggtctat tttaataact aaaaatgatt aacgtatagt catttttctt
    14461 attctgatga aattgataat ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt
    14521 tggaatagaa gcatcaaact tttgaatgga ttgaacaata agacgaatcc gccaatcaac
    14581 aacagaatac tactataaac actccaactt ttagcttttg tattcatgct gaagaacaac
    14641 tggaacaagc tgttgatgat aacagctaat ccaagaagaa tcggcaggat agaaacgatt
    14701 gcaggtgcaa aagcatagac gatcagagct aagatcagga aaaaaagacc actgaacaga
    14761 ccgatactcc aagaacctgt tttcctttta aaacggtaag cctcaaaaag attcaacaac
    14821 ccaagcaaaa caagataggc ggtgatcaga taaccgataa aatgaaaaac agcagaaggg
    14881 tttagcacaa tcgcaatccc agaaataata taaacagcag ctcttaataa ggcgtatttt
    14941 tgaatatttt gtttaatcgt ctcaaacata taagaaaacc tcctttgact tattttaacg
    15001 tgaactagtc attttaccaa attaaatgtt acaaaatgag cgaaggagaa gaatatctct
    15061 ggaaaaatca acttctgagg atcttttcca tttttttgcc ttttgctagc tcatcgatca
    15121 ttttatctaa ataacgaatt tctttcatca agttatcctc gatgtcttct actcgtacac
    15181 cacaaacaac acctgtaatc aaggaacgtg aaggattggg gacaggggct tccgcaaaaa
    15241 attgttcgaa agtcacttgt ttttgtagtt gatattctag ttctgtttgg gaataacccg
    15301 tcagccaaca aatgatttca tctacttcct cttttatacg atcttttctc tccgcttttg
    15361 ctacatacat aggataaacg cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa
    15421 tcagtcacct ttcttttttt caaattattt actacttata gtatataaga aaaagagaag
    15481 aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt
    15541 tttccgctct ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg
    15601 ctcctaaagc tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat
    15661 ctgtacaatc accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca
    15721 ctatttcatt tcttggattc atttcgatcg ttctatctag ccaatccgta ttagggagta
    15781 agccgactag aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat
    15841 cgatatagga gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt
    15901 tgataacagt gacattcgca agagagacta gtttatcctg taagatttga tctgctttta
    15961 attctggtaa gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg
    16021 cttcaacacc agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc
    16081 cgtcacagtg tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat
    16141 tgatagaacg ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac
    16201 cgttagctaa gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat
    16261 tcatcataat gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg
    16321 gaccttctgt gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc
    16381 cgccgaaaga atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg
    16441 cactactagc tgctggtcca ccgccaatca ctaatacatc ataaggttct ttttcaaaca
    16501 actcttcttc aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc
    16561 cgctactaaa ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat
    16621 cgatttcttt ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata
    16681 cagctaatat attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat
    16741 aactgtcaaa atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga
    16801 ttttcggtgc ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac
    16861 cgagggggat accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac
    16921 taggtgtgcg catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt
    16981 ccttcgtaaa ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa
    17041 caataggaga ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta
    17101 acatatccac cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac
    17161 tttctttcca ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc
    17221 gaatcttatc aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag
    17281 cttgaacgat tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt
    17341 catccaaaac atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt
    17401 cgattttacc gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg
    17461 tagagcaaga aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt
    17521 cttctaattc agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc
    17581 agttccccat aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg
    17641 cattaaattc ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta
    17701 tttatttata attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt
    17761 aataaagatt actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg
    17821 ctactgctgg aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat
    17881 gtgtaagtgc aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt
    17941 gagtaaatca taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg
    18001 taaccagcgg aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt
    18061 tcttcctgca atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg
    18121 ttcgtcattt attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg
    18181 tgtttgtgtg tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg
    18241 gacaaagcga gcattttctg cagaaacaag attaattttg gaaacatacg taccactttg
    18301 aggtatcgta tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt
    18361 ccggccgata gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa
    18421 gtcagcatag ataatttgtt tacgaatcgc ttgataagct tgtgattgca agctgctttt
    18481 catctttatt cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa
    18541 caatctggta tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat
    18601 agcgattttt attgcataaa attcacttga atacaaaaac taatatgtta gtttaggagg
    18661 ggaaaaatga ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa
    18721 gtagaaatac ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc
    18781 tggatccatt tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt
    18841 ttagcggatc taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa
    18901 ataattgaaa gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc
    18961 agtggagagt tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca
    19021 gattgttctg aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta
    19081 gttacttttg ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata
    19141 ggaccgattg cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt
    19201 cttgctgtgc tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct
    19261 acagataatt tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa
    19321 gaatggcaaa aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa
    19381 attgttgcgt ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt
    19441 agcaaaatgt tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga
    19501 aataatattg ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat
    19561 tttcccaatg gacgtccgaa attcgaaaaa gctggggtat tcttgactga tcgtgcaacg
    19621 gtagataaag cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta
    19681 gctattatgg gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat
    19741 ttatcgctgc tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca
    19801 ggtatcattg atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca
    19861 aatattccta atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt
    19921 tatggtgaga cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta
    19981 ttcataccat tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga
    20041 aaaccattca tcccgagtcc agacccacta ttgacagacc ttcagcataa gctttccgat
    20101 attcaactgg gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca
    20161 aataaagaaa tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa
    20221 atgttcatca agatggtagc aggaccaaat gccgtacgaa aaacattaca ggattactta
    20281 caagaacatg gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag
    20341 aaaagattac cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat
    20401 tgatggatat accagtagga gaggtatggc ctagggagag gatcaaagaa ctaaaagaag
    20461 aaattgagtc tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca
    20521 ttaaaattgg attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa
    20581 acttggcaga atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg
    20641 tgaaatctga ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg
    20701 atgatattcc agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat
    20761 ttggattgcc gggatgggaa cctgagcgct tagctcctat caaatccttg cttgaagcct
    20821 atgcatcagt agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca
    20881 tcccaacatg tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact
    20941 cgatttttgg attagctcga atcgtaaaga atcgagaaga tttagattgg ttgtgtcgca
    21001 tagtagacag cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag
    21061 aaaatgatgt atatgggatt ttagcagaat tttgccaaag agatcgaatc ccatttgcac
    21121 atgttcgaaa tattaaattt ttagctaatc gagatttcta tgaggcacca cataaatccg
    21181 aatacggttc attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg
    21241 gatacatccg tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg
    21301 gcttatatga tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa
    21361 aaggaaacaa aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga
    21421 aggatgttca aattcttgat acataaatcc ggaggttgtt aataagcatt cggatttatg
    21481 tatcttattt ggtctaggaa gtaacttgat tttctttttt cgattgatcc atgagtgctt
    21541 cttcttttaa ttcaaagata tattctgaga tcctcataat cacttttttc cagtcttctg
    21601 gtgtgatgat tccttttaaa atatagattt ttttgatatg agaaggcaat tgtttcaaag
    21661 ggcagtcgct gataacgatg ttgcagttct ccataaaatc attttgtatt ttgatcgatt
    21721 catttgaaaa aatttgttct atcttaacga tcagatcttt actgatataa aaatcacgcg
    21781 tatagttcac accaatcaca attcgcggct gttttttgta gcgatcatac aaatgtacta
    21841 ggtcttcaac catccattgc acattttcct tttctaaaat aactggtaac aagtgttgat
    21901 tttgttctct gaaatccata taaaattgtt caatggcggt ttggacttct ttcaatacgc
    21961 tatgttcttg ataaagcatg aaagtaggaa ggggcgtatt tggttgattg aagttaagaa
    22021 atccattata aagtttattt cgtgcgaatg ccaatgagta aagaatcttt tctttttcag
    22081 gtataaacaa atcaaataca gtacaaaggg actccaccat ttctcttgaa taaagaacat
    22141 gaggaacgtt tgcttttttt aaaagtttgt attgctccaa gcttgtttct tccgattcga
    22201 ttttcgtgat agaaagtcga gctaaaatat tcactaagat gcgttgttcg cgacaaattg
    22261 gcagattagg tcgcaaaata tctacagaga gatcaataaa aaagtcaata tattcatact
    22321 ctgtgaattc tttttgtacg tctttcacag aagttgcagg aaaattctct ttacatattt
    22381 ttaatagaaa atataattta tctatcatac cgctatttag tcggtcaagt acttcttttt
    22441 taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag
    22501 cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt
    22561 ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg tactatgcta
    22621 aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct
    22681 gactgaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac
    22741 tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc
    22801 cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga
    22861 gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat
    22921 ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa
    22981 aaatatctaa ttttgtattg ctttttttct tcaagataaa cgttttaatc gcttcttgag
    23041 tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt
    23101 tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt
    23161 cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc
    23221 aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagac
    23281 agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga
    23341 tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat
    23401 caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag ctgttacatt
    23461 tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga ctatccaaca
    23521 caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct
    23581 ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc cgtatagtca
    23641 gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg ttgaagaacc
    23701 aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa atccagacgg
    23761 agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc aaccagagcc
    23821 agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa ctccagaacc
    23881 agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag ataatgaaac
    23941 tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga ctccagaacc
    24001 agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa caattcaaga
    24061 ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca
    24121 gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct taaaaagtag
    24181 caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa atccggagag
    24241 tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt
    24301 tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc
    24361 atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat tgaaatctga
    24421 tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct
    24481 tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc
    24541 ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca
    24601 aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa
    24661 gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct
    24721 ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg
    24781 atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca
    24841 gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg agcttctaat
    24901 accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa
    24961 gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata
    25021 aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt
    25081 aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca
    25141 atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa
    25201 tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat atactttgtg
    25261 aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta
    25321 gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata
    25381 aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat
    25441 aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa
    25501 tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg gaatatgaaa
    25561 ataagaaaga gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt
    25621 ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta
    25681 tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga
    25741 taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagga
    25801 gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt
    25861 ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt
    25921 cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg
    25981 gtataggtaa gattcagcgg cttgaggagg gaagtgtagt gtctaatgat ttccttagag
    26041 gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct
    26101 ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg
    26161 ctttcagagt aacttctata tttccagctg tagcttttga atacggacac actttatgcg
    26221 ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct
    26281 ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag
    26341 tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg
    26401 cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag
    26461 gagctgcaat attcaattcg aatgtacgat cgggattatg aacttctcca gagcgtccgc
    26521 ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct ttaattatat
    26581 tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa
    26641 gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa
    26701 aaaatatcag agacaaaaac taatctcatc ataaacatta catatatttc tcaaaaagtt
    26761 gttttctttc ttcaaaactt acttcacgat ggacctgatg aagcatccat aaataaccaa
    26821 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
    26881 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
    26941 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
    27001 tttcatccag catatggaat ctcgtgccat acaaggtaaa aatgacttca ttattgcctc
    27061 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
    27121 gtgctttcaa actatcattt aatacaaaat ctatttccgt tcctttatgc ataaagatct
    27181 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagtta aagaaaacgc
    27241 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
    27301 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
    27361 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
    27421 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
    27481 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
    27541 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
    27601 cataaagaaa ctcttctatt ccaaaaacaa acagattaag agaaacaacg aatgcgtctg
    27661 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
    27721 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
    27781 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
    27841 ttgcaatgat taaaggaaaa aacaagcttc cgaaaagtac tttaaaaaag ggttttttac
    27901 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
    27961 ggtcaaatcc taaaacagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
    28021 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
    28081 tcttaatgta tgtccatata aattgtctcg tttctttcat ttatccactt ccttaattca
    28141 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
    28201 aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgtta
    28261 aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt
    28321 caggacacta aatagtacag gaagtagatt aaaactttta aaagttttaa gtctatctat
    28381 ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga
    28441 aagcgttgtc ttttgtttct ctatcactta tagtgatgac gtcatattaa aacacaagga
    28501 ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat
    28561 atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat
    28621 atgtgctttg tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac
    28681 caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg
    28741 aaacaaggcg cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt
    28801 acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt
    28861 ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa
    28921 cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg
    28981 aacacacttt accttgggga agtaaagatc cagaccgaga agacttcttc gggggagatt
    29041 tgcaaggtgt gagggatcat ttggattact tgacggacct tggcgtcaat ggcatctatt
    29101 tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg
    29161 tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa
    29221 gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt
    29281 ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt
    29341 ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat
    29401 atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc
    29461 aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc
    29521 gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta
    29581 cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct caagcttggc
    29641 tgcaagggga tgaattccat gcagtgatga attatgcatt cacagattcg attaaagatt
    29701 attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa
    29761 tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg
    29821 ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta
    29881 tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg
    29941 aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc
    30001 tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg
    30061 aaggttctac tcagtggcta atagtatatg atcgtgaaga taagctctat ttcacacggg
    30121 aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag
    30181 aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag
    30241 agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa
    30301 agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac
    30361 acaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc
    30421 tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga
    30481 agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa
    30541 cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga
    30601 catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt
    30661 tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt
    30721 aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg
    30781 aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt
    30841 agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt
    30901 tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta
    30961 ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa
    31021 tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga
    31081 ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc
    31141 cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca
    31201 aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg
    31261 acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa
    31321 caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt
    31381 ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa
    31441 aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa
    31501 catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc
    31561 aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat
    31621 agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc
    31681 ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt
    31741 aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca
    31801 agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt
    31861 gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt
    31921 cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca
    31981 atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc
    32041 attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt
    32101 caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa
    32161 ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac gatcattttc
    32221 ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca tattccacca
    32281 gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca
    32341 tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca
    32401 actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa
    32461 ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact
    32521 atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt
    32581 cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg
    32641 aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat
    32701 gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc
    32761 gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg
    32821 gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc catgatctat
    32881 ctgtttaaca atggtggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt
    32941 atttcctgga tctataaact tacgactgga acttctccgc agtattctat ggcttcagca
    33001 atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca
    33061 aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa
    33121 aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct
    33181 atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt
    33241 tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt
    33301 acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga
    33361 ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa
    33421 gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt
    33481 atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata
    33541 taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa
    33601 tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga
    33661 ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac
    33721 cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg
    33781 ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg
    33841 ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact
    33901 ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat
    33961 ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac
    34021 tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc
    34081 ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg
    34141 ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa
    34201 ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt
    34261 ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat
    34321 gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat
    34381 ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat
    34441 tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc
    34501 tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa
    34561 aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta
    34621 tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga
    34681 tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa
    34741 gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga
    34801 aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg
    34861 attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt
    34921 tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa
    34981 attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa
    35041 tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcttct ttggcactag
    35101 gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg
    35161 atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa
    35221 agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag
    35281 gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca
    35341 atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca
    35401 aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga
    35461 tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac
    35521 aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat
    35581 tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta
    35641 tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat
    35701 tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa
    35761 atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag
    35821 ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact
    35881 accaattgac attagaacat tggacaaaga attttcatca agtccaagat caagtcacga
    35941 aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt
    36001 catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg
    36061 acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag
    36121 aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc
    36181 taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta
    36241 aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag
    36301 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
    36361 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
    36421 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
    36481 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
    36541 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
    36601 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
    36661 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
    36721 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
    36781 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
    36841 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
    36901 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
    36961 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
    37021 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
    37081 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
    37141 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
    37201 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
    37261 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
    37321 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
    37381 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
    37441 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
    37501 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
    37561 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
    37621 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
    37681 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
    37741 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
    37801 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
    37861 gtacagagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
    37921 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
    37981 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
    38041 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
    38101 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
    38161 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
    38221 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
    38281 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
    38341 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
    38401 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
    38461 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
    38521 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
    38581 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
    38641 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
    38701 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
    38761 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
    38821 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
    38881 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
    38941 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
    39001 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
    39061 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
    39121 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
    39181 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
    39241 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
    39301 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
    39361 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
    39421 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
    39481 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
    39541 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
    39601 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
    39661 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
    39721 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
    39781 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
    39841 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
    39901 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
    39961 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    40021 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    40081 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    40141 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    40201 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    40261 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    40321 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    40381 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    40441 ccctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    40501 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga
    40561 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    40621 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc
    40681 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    40741 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    40801 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    40861 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    40921 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    40981 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    41041 aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa
    41101 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    41161 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    41221 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    41281 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    41341 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    41401 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    41461 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    41521 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    41581 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    41641 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa
    41701 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    41761 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    41821 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    41881 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    41941 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    42001 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    42061 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    42121 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    42181 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    42241 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    42301 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    42361 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    42421 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    42481 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    42541 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    42601 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    42661 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    42721 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    42781 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    42841 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    42901 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    42961 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    43021 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    43081 agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac
    43141 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    43201 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    43261 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    43321 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    43381 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    43441 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    43501 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    43561 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    43621 cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt
    43681 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    43741 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    43801 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    43861 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    43921 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    43981 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    44041 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    44101 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    44161 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    44221 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
    44281 caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata
    44341 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
    44401 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
    44461 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
    44521 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
    44581 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
    44641 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
    44701 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
    44761 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
    44821 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
    44881 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
    44941 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
    45001 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
    45061 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
    45121 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
    45181 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
    45241 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
    45301 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
    45361 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
    45421 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
    45481 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
    45541 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
    45601 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
    45661 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
    45721 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
    45781 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
    45841 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
    45901 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
    45961 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
    46021 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
    46081 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
    46141 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
    46201 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
    46261 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    46321 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    46381 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    46441 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    46501 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    46561 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    46621 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    46681 caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    46741 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    46801 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    46861 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    46921 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    46981 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    47041 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    47101 aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt
    47161 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    47221 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    47281 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    47341 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    47401 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    47461 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    47521 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    47581 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    47641 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    47701 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    47761 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    47821 tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct
    47881 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    47941 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    48001 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    48061 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    48121 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    48181 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    48241 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    48301 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    48361 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    48421 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    48481 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    48541 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    48601 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    48661 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    48721 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    48781 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    48841 aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    48901 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    48961 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    49021 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    49081 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    49141 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    49201 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    49261 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    49321 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    49381 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    49441 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    49501 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    49561 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    49621 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    49681 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    49741 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    49801 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    49861 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    49921 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    49981 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    50041 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    50101 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    50161 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    50221 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    50281 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    50341 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    50401 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    50461 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    50521 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    50581 tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    50641 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    50701 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    50761 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    50821 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    50881 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    50941 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    51001 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    51061 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    51121 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    51181 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    51241 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    51301 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    51361 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    51421 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    51481 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    51541 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    51601 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    51661 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    51721 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    51781 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    51841 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    51901 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    51961 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    52021 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    52081 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    52141 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    52201 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    52261 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    52321 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    52381 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    52441 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    52501 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    52561 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    52621 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    52681 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    52741 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    52801 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    52861 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    52921 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    52981 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    53041 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    53101 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    53161 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    53221 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    53281 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    53341 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    53401 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    53461 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    53521 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    53581 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    53641 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    53701 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    53761 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    53821 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    53881 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    53941 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    54001 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    54061 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    54121 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    54181 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    54241 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    54301 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    54361 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    54421 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    54481 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    54541 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    54601 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    54661 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    54721 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    54781 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    54841 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    54901 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    54961 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    55021 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    55081 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    55141 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    55201 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    55261 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    55321 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    55381 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    55441 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    55501 atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta
    55561 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    55621 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    55681 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    55741 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    55801 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    55861 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    55921 aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc
    55981 gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc
    56041 ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta
    56101 cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa
    56161 aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa
    56221 atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg
    56281 atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat
    56341 taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc
    56401 aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg
    56461 gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag
    56521 acatcatttt gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc
    56581 gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg
    56641 gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa
    56701 gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc
    56761 ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga
    56821 aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact
    56881 tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt
    56941 attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca
    57001 aacagataca cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt
    57061 ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc
    57121 aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt
    57181 agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc
    57241 gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg
    57301 cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt
    57361 aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga
    57421 acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat
    57481 tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg
    57541 ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta
    57601 tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc
    57661 tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg
    57721 tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg
    57781 aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc
    57841 tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc
    57901 tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca
    57961 aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat
    58021 caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga
    58081 aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca
    58141 catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt tactggataa
    58201 tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa
    58261 agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc
    58321 tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga
    58381 agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt
    58441 gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta
    58501 taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag
    58561 gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc
    58621 cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta
    58681 tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg
    58741 aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg
    58801 tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact
    58861 gattgcccgc tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag
    58921 cttttcgttt ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa
    58981 gagggaccgt taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca
    59041 gtaggctatc aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac
    59101 atcaatggga tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg
    59161 atcaatccga tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa
    59221 gatatagaag agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat
    59281 gaattaggga tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa
    59341 tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc
    59401 gacggggtag atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg
    59461 gagtattctc atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac
    59521 ttaaattggg aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg
    59581 gataaaggga tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat
    59641 tcagtaaaag ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa
    59701 catttgaccg atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca
    59761 agtggtgtca ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg
    59821 atattcgaat ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc
    59881 cttaaattga aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac
    59941 gctttatata tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt
    60001 cccgattttt gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt
    60061 acgccattta tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc
    60121 gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag
    60181 acaagagaag aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc
    60241 atgcaatgga cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact
    60301 aatccaaata ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg
    60361 caattctata agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct
    60421 tataaagaat atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat
    60481 gaacaatatc ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa
    60541 gaagcagatc agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct
    60601 aaaggcatac tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc
    60661 gttaaagttc attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc
    60721 atttcaataa ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac
    60781 gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg
    60841 cataccgatt gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc
    60901 gacaggatca ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc
    60961 aggaacaaca gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa
    61021 tgctcggttc aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa
    61081 ctcttttaac gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg
    61141 tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg
    61201 tgcctcagcc aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga
    61261 caacttcatt ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt
    61321 gaagacttgc agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc
    61381 ctggtataga agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc
    61441 cgatgatcat aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt
    61501 tgctgaaaga accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc
    61561 actgcggata tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc
    61621 cacccgttgg accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa
    61681 atccattcgt agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa
    61741 tcagcatagc atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata
    61801 ccgccagacc agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg
    61861 gcaacaaatt ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc
    61921 gctgttattg cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt
    61981 ttatgagaaa atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca
    62041 gccatccttg tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta
    62101 gcgagatgga tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct
    62161 tctgccaaat taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa
    62221 atatacaatt gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg
    62281 attgggatat tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa
    62341 ttcaataacc caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt
    62401 cgtttaagca attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc
    62461 cctatagaag tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct
    62521 gtttggattc ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata
    62581 acattttcct ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg
    62641 gcatattttt atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc
    62701 tttctttcct taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt
    62761 tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc
    62821 aagcgaggaa gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga
    62881 gctaaagcaa taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc
    62941 aaaaaatcta acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg
    63001 atcaaatcca cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa
    63061 tctccgctat cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa
    63121 taagtcaaag cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg
    63181 acattcccaa ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata
    63241 tctcttgcat cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg
    63301 cattcacttt ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa
    63361 aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc
    63421 gaaaaagctt ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg
    63481 gtctctagtg caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg
    63541 tttgctaaag gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat
    63601 ctggaatgca ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa
    63661 actgtttttt gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta
    63721 gggataccag ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat
    63781 gattgagaaa cagacaaagc aggttgttcc attaaataaa gaataggaa
//
LOCUS       DAJSVP010000009        56525 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSVP010000009 DAJSVP010000000
VERSION     DAJSVP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.009"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..1666
                     /locus_tag="ON449_000540"
     CDS             35..1666
                     /locus_tag="ON449_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3933157.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1728..2369)
                     /locus_tag="ON449_000541"
     CDS             complement(1728..2369)
                     /locus_tag="ON449_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3933158.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2555..2631
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2651..2959
                     /gene="rplU"
                     /locus_tag="ON449_000542"
     CDS             2651..2959
                     /gene="rplU"
                     /locus_tag="ON449_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3933159.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2986..3330
                     /locus_tag="ON449_000543"
     CDS             2986..3330
                     /locus_tag="ON449_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3933160.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3343..3636
                     /gene="rpmA"
                     /locus_tag="ON449_000544"
     CDS             3343..3636
                     /gene="rpmA"
                     /locus_tag="ON449_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3933161.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4070..6877
                     /locus_tag="ON449_000545"
     CDS             4070..6877
                     /locus_tag="ON449_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3933162.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7068..7559
                     /locus_tag="ON449_000546"
     CDS             7068..7559
                     /locus_tag="ON449_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3933163.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7662..8651
                     /gene="manX"
                     /locus_tag="ON449_000547"
     CDS             7662..8651
                     /gene="manX"
                     /locus_tag="ON449_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3933164.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8684..9487
                     /locus_tag="ON449_000548"
     CDS             8684..9487
                     /locus_tag="ON449_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3933165.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9508..10422
                     /locus_tag="ON449_000549"
     CDS             9508..10422
                     /locus_tag="ON449_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3933166.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10505..10879
                     /locus_tag="ON449_000550"
     CDS             10505..10879
                     /locus_tag="ON449_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3933167.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11018..11263
                     /locus_tag="ON449_000551"
     CDS             11018..11263
                     /locus_tag="ON449_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3933168.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11435..12499
                     /locus_tag="ON449_000552"
     CDS             11435..12499
                     /locus_tag="ON449_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3933169.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12745..13170
                     /locus_tag="ON449_000553"
     CDS             12745..13170
                     /locus_tag="ON449_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3933170.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13163..13612
                     /gene="nusB"
                     /locus_tag="ON449_000554"
     CDS             13163..13612
                     /gene="nusB"
                     /locus_tag="ON449_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3933171.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13709..14563
                     /locus_tag="ON449_000555"
     CDS             13709..14563
                     /locus_tag="ON449_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3933172.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14564..15901
                     /locus_tag="ON449_000556"
     CDS             14564..15901
                     /locus_tag="ON449_000556"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3933173.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15906..16130
                     /locus_tag="ON449_000557"
     CDS             15906..16130
                     /locus_tag="ON449_000557"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3933174.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16132..17022
                     /locus_tag="ON449_000558"
     CDS             16132..17022
                     /locus_tag="ON449_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3933175.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17031..17849
                     /locus_tag="ON449_000559"
     CDS             17031..17849
                     /locus_tag="ON449_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3933176.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17956..18405
                     /locus_tag="ON449_000560"
     CDS             17956..18405
                     /locus_tag="ON449_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3933177.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18423..20105
                     /gene="recN"
                     /locus_tag="ON449_000561"
     CDS             18423..20105
                     /gene="recN"
                     /locus_tag="ON449_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3933178.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20160..20276)
                     /locus_tag="ON449_000562"
     CDS             complement(20160..20276)
                     /locus_tag="ON449_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3933179.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20349..21305)
                     /locus_tag="ON449_000563"
     CDS             complement(20349..21305)
                     /locus_tag="ON449_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3933180.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21476..21844
                     /locus_tag="ON449_000564"
     CDS             21476..21844
                     /locus_tag="ON449_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3933181.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22014..22445
                     /gene="mraZ"
                     /locus_tag="ON449_000565"
     CDS             22014..22445
                     /gene="mraZ"
                     /locus_tag="ON449_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3933182.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22466..23425
                     /gene="rsmH"
                     /locus_tag="ON449_000566"
     CDS             22466..23425
                     /gene="rsmH"
                     /locus_tag="ON449_000566"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3933183.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23430..23831
                     /gene="ftsL"
                     /locus_tag="ON449_000567"
     CDS             23430..23831
                     /gene="ftsL"
                     /locus_tag="ON449_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3933184.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23832..26024
                     /locus_tag="ON449_000568"
     CDS             23832..26024
                     /locus_tag="ON449_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3933185.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26050..27012
                     /locus_tag="ON449_000569"
     CDS             26050..27012
                     /locus_tag="ON449_000569"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3933186.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27026..28387
                     /locus_tag="ON449_000570"
     CDS             27026..28387
                     /locus_tag="ON449_000570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3933187.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28413..29501
                     /gene="murG"
                     /locus_tag="ON449_000571"
     CDS             28413..29501
                     /gene="murG"
                     /locus_tag="ON449_000571"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3933188.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29516..30736
                     /locus_tag="ON449_000572"
     CDS             29516..30736
                     /locus_tag="ON449_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3933189.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30892..32217
                     /gene="ftsA"
                     /locus_tag="ON449_000573"
     CDS             30892..32217
                     /gene="ftsA"
                     /locus_tag="ON449_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3933190.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32240..33481
                     /gene="ftsZ"
                     /locus_tag="ON449_000574"
     CDS             32240..33481
                     /gene="ftsZ"
                     /locus_tag="ON449_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3933191.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33498..34175
                     /locus_tag="ON449_000575"
     CDS             33498..34175
                     /locus_tag="ON449_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3933192.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34189..34788
                     /locus_tag="ON449_000576"
     CDS             34189..34788
                     /locus_tag="ON449_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3933193.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34803..35090
                     /locus_tag="ON449_000577"
     CDS             34803..35090
                     /locus_tag="ON449_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3933194.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35139..35921
                     /locus_tag="ON449_000578"
     CDS             35139..35921
                     /locus_tag="ON449_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3933195.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35942..36649
                     /locus_tag="ON449_000579"
     CDS             35942..36649
                     /locus_tag="ON449_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3933196.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36911..39709
                     /gene="ileS"
                     /locus_tag="ON449_000580"
     CDS             36911..39709
                     /gene="ileS"
                     /locus_tag="ON449_000580"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3933197.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39758..41284)
                     /locus_tag="ON449_000581"
     CDS             complement(39758..41284)
                     /locus_tag="ON449_000581"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3933198.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41299..41946)
                     /locus_tag="ON449_000582"
     CDS             complement(41299..41946)
                     /locus_tag="ON449_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3933199.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42130..42459)
                     /locus_tag="ON449_000583"
     CDS             complement(42130..42459)
                     /locus_tag="ON449_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3933200.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42636..43364
                     /gene="sfsA"
                     /locus_tag="ON449_000584"
     CDS             42636..43364
                     /gene="sfsA"
                     /locus_tag="ON449_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3933201.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43380..44393
                     /locus_tag="ON449_000585"
     CDS             43380..44393
                     /locus_tag="ON449_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3933202.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44393..45670
                     /locus_tag="ON449_000586"
     CDS             44393..45670
                     /locus_tag="ON449_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3933203.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45733..48435
                     /locus_tag="ON449_000587"
     CDS             45733..48435
                     /locus_tag="ON449_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3933204.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48587..48904
                     /locus_tag="ON449_000588"
     CDS             48587..48904
                     /locus_tag="ON449_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3933205.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48934..49254
                     /locus_tag="ON449_000589"
     CDS             48934..49254
                     /locus_tag="ON449_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3933206.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49362..50822
                     /locus_tag="ON449_000590"
     CDS             49362..50822
                     /locus_tag="ON449_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3933207.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50890..51111
                     /locus_tag="ON449_000591"
     CDS             50890..51111
                     /locus_tag="ON449_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933208.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51142..51324
                     /locus_tag="ON449_000592"
     CDS             51142..51324
                     /locus_tag="ON449_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3933209.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51324..51737
                     /locus_tag="ON449_000593"
     CDS             51324..51737
                     /locus_tag="ON449_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3933210.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51860..53041
                     /locus_tag="ON449_000594"
     CDS             51860..53041
                     /locus_tag="ON449_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933211.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53112..53276)
                     /locus_tag="ON449_000595"
     CDS             complement(53112..53276)
                     /locus_tag="ON449_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933212.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53430..53503
                     /locus_tag="ON449_000596"
     tRNA            53430..53503
                     /locus_tag="ON449_000596"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53464..53466,aa:Arg,seq:cct)
     gene            complement(53572..54711)
                     /locus_tag="ON449_000597"
     CDS             complement(53572..54711)
                     /locus_tag="ON449_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3933213.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(55010..55645)
                     /locus_tag="ON449_000598"
     CDS             complement(55010..55645)
                     /locus_tag="ON449_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933214.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55758..56393)
                     /locus_tag="ON449_000599"
     CDS             complement(55758..56393)
                     /locus_tag="ON449_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3933215.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(56427..>56525)
                     /locus_tag="ON449_000600"
     CDS             complement(56427..>56525)
                     /locus_tag="ON449_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HCR3933216.1"
                     /translation="SADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        18539 a         9594 c        12026 g        16366 t
ORIGIN      
        1 ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact
       61 aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat
      121 cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga
      181 tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg
      241 cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc
      301 ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact
      361 tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac
      421 ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg
      481 tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt
      541 tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta
      601 tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc
      661 agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat
      721 cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt
      781 tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc
      841 tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg
      901 aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag
      961 cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt
     1021 actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc cagcacttgc
     1081 aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt
     1141 tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat
     1201 ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt
     1261 caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc
     1321 agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat
     1381 gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt
     1441 ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca
     1501 agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga
     1561 aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca
     1621 tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga
     1681 acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt
     1741 aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag
     1801 aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag
     1861 gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg
     1921 gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt
     1981 aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg
     2041 gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc
     2101 cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt
     2161 gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga
     2221 acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga
     2281 agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct
     2341 tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga
     2401 ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa
     2461 gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg
     2521 ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag
     2581 taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt
     2641 gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg
     2701 acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga
     2761 agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac
     2821 tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa
     2881 acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat
     2941 cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca
     3001 tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct
     3061 ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc
     3121 aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc
     3181 ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag
     3241 attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat
     3301 atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat
     3361 ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa
     3421 tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt
     3481 taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac
     3541 actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa
     3601 caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt
     3661 gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt agtggaggct
     3721 acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt
     3781 tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt
     3841 atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg
     3901 ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa
     3961 atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt
     4021 ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa
     4081 agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat
     4141 tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg
     4201 cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt
     4261 tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga
     4321 agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa
     4381 cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg
     4441 attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat
     4501 gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa
     4561 ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa
     4621 aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg
     4681 tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta
     4741 ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa
     4801 tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt
     4861 tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa
     4921 agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc
     4981 tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca
     5041 attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc
     5101 cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt
     5161 agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt
     5221 agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct
     5281 atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc
     5341 aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca
     5401 tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga
     5461 agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa
     5521 ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga
     5581 agaacggcat acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc
     5641 tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag
     5701 tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag
     5761 aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt
     5821 ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc
     5881 gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt
     5941 catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac
     6001 tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg
     6061 aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg
     6121 aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa
     6181 aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt
     6241 gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat
     6301 tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac
     6361 tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca
     6421 aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt
     6481 catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt
     6541 catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc
     6601 tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac
     6661 agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt
     6721 gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga
     6781 agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca
     6841 taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt
     6901 atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa
     6961 aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga
     7021 aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat
     7081 tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa
     7141 caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt
     7201 ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa
     7261 tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca
     7321 atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg
     7381 gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac
     7441 aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag
     7501 tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc
     7561 aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat
     7621 ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag
     7681 caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac
     7741 aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca
     7801 aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt
     7861 tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat
     7921 gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt
     7981 caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa
     8041 aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac
     8101 aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat
     8161 tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag
     8221 tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga
     8281 gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa
     8341 tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa
     8401 atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg
     8461 ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag
     8521 aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag
     8581 tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag
     8641 caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat
     8701 aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca
     8761 atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc
     8821 tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc
     8881 cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg
     8941 tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc
     9001 agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga
     9061 tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg
     9121 tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac
     9181 tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg
     9241 gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt
     9301 atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc
     9361 tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg
     9421 ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga
     9481 ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa
     9541 gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt
     9601 atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca
     9661 aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac
     9721 attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg
     9781 cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt
     9841 gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca
     9901 ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt
     9961 cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact
    10021 gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg
    10081 ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattctcacc agttgtatca
    10141 actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa
    10201 ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta
    10261 acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca
    10321 ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta
    10381 ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc
    10441 gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga
    10501 gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac
    10561 gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt tttaccatgc
    10621 gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc
    10681 agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac
    10741 atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga
    10801 tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag
    10861 tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt
    10921 ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca
    10981 tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat
    11041 tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg
    11101 ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg
    11161 cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat
    11221 tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta
    11281 aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa
    11341 aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg
    11401 gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg
    11461 caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg
    11521 ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt
    11581 ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat
    11641 tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact
    11701 gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga
    11761 aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa
    11821 agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga
    11881 tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga
    11941 cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg
    12001 attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt
    12061 gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt
    12121 cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca
    12181 attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc
    12241 tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg
    12301 gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt
    12361 accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg
    12421 tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa
    12481 agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac
    12541 agactttgtt atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg
    12601 taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg
    12661 cagttttctt accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc
    12721 ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac
    12781 caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca
    12841 gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa
    12901 ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg
    12961 aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa
    13021 atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc
    13081 aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt
    13141 tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat
    13201 ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat
    13261 tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt
    13321 tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa
    13381 agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt
    13441 ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt
    13501 tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt
    13561 cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt aagtcctagc
    13621 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
    13681 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
    13741 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
    13801 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
    13861 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
    13921 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
    13981 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
    14041 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
    14101 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
    14161 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
    14221 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
    14281 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
    14341 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
    14401 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
    14461 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
    14521 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
    14581 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
    14641 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
    14701 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
    14761 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
    14821 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
    14881 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
    14941 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
    15001 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
    15061 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
    15121 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
    15181 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
    15241 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
    15301 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
    15361 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
    15421 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
    15481 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
    15541 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
    15601 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
    15661 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
    15721 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
    15781 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
    15841 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
    15901 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
    15961 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
    16021 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
    16081 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
    16141 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
    16201 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
    16261 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
    16321 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
    16381 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
    16441 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
    16501 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
    16561 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
    16621 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
    16681 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
    16741 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
    16801 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
    16861 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
    16921 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
    16981 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
    17041 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
    17101 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
    17161 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
    17221 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
    17281 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
    17341 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
    17401 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
    17461 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
    17521 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
    17581 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
    17641 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
    17701 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
    17761 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
    17821 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
    17881 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
    17941 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
    18001 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
    18061 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
    18121 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
    18181 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
    18241 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
    18301 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
    18361 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
    18421 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
    18481 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
    18541 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
    18601 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
    18661 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
    18721 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
    18781 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
    18841 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
    18901 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
    18961 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
    19021 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
    19081 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
    19141 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
    19201 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
    19261 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
    19321 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
    19381 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
    19441 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
    19501 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
    19561 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
    19621 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
    19681 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
    19741 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
    19801 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
    19861 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
    19921 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
    19981 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
    20041 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
    20101 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat attttatttt
    20161 tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat cagcattgtc
    20221 caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc acgcattgtt
    20281 ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat ttccacaaga
    20341 gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat acaaataaat
    20401 aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg taacttaatg
    20461 ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt taaagaagtt
    20521 aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt ctgattcatt
    20581 tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt ttccaccaat
    20641 acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt ttgcgctagt
    20701 tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag gcttttttgg
    20761 atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt ggcttccaaa
    20821 gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt gacctgccaa
    20881 atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa ggtctggcta
    20941 aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt ctttttgggc
    21001 gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg acttgtttta
    21061 gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg attgaattct
    21121 tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga cggaatttca
    21181 aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca ataccagatc
    21241 gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt aaaatatttg
    21301 atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga attggaatac
    21361 atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa atgaaacagt
    21421 tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta cgttcatggg
    21481 cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt tgtttggttg
    21541 ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt
    21601 ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag ggacaatttc
    21661 tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg ctgttttcca
    21721 agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt ggcgtttggt
    21781 ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac tttcttacct
    21841 atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt
    21901 aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag gtggtagaaa
    21961 gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt tcaatgttga
    22021 tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc
    22081 gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct
    22141 atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa
    22201 aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata
    22261 aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt
    22321 gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt
    22381 tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac
    22441 tataagaata ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt
    22501 gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact
    22561 cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta
    22621 tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt
    22681 cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag
    22741 tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct
    22801 agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga
    22861 tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt
    22921 aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga
    22981 acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca
    23041 agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc
    23101 tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat
    23161 ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag
    23221 aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc
    23281 tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc
    23341 ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga
    23401 aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg
    23461 aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga
    23521 atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg
    23581 aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta
    23641 tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca
    23701 atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg
    23761 aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga
    23821 aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa
    23881 atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt
    23941 tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct
    24001 cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag
    24061 gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta
    24121 aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga
    24181 attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac
    24241 tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg
    24301 ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg
    24361 tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt
    24421 ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag
    24481 gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa
    24541 aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg
    24601 gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg
    24661 atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa
    24721 ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat
    24781 tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat
    24841 caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg
    24901 aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg
    24961 gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg
    25021 tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg
    25081 gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct acattaaata
    25141 ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga
    25201 tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca
    25261 aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac
    25321 ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg
    25381 aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa
    25441 acggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat acagcctacc
    25501 tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg tatctaacaa
    25561 tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat ccattgatga
    25621 aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca aaaacagaaa
    25681 aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa
    25741 aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa caatctactg
    25801 ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt ggtgataagc
    25861 tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg aaaatcctcg
    25921 gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt gctccatatg
    25981 aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag aaataggaga
    26041 gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg tgcaatgaca
    26101 attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg tcaggcgatt
    26161 cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat gggcggctta
    26221 gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca aaaacagcta
    26281 actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat cggctttctt
    26341 gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa gcagaaattg
    26401 attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga aggttatcca
    26461 ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg tattttcgct
    26521 ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat cgatggatta
    26581 gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg gcatcagcat
    26641 cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg tttcttcgtc
    26701 tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc gttgggcgga
    26761 cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt gattggtttg
    26821 atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa gcttacagga
    26881 aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg atggtcggag
    26941 tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat cacattatgg
    27001 atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa ataagaaagt
    27061 gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac atgaattggg
    27121 cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag cacaagagct
    27181 gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt tggatgaaga
    27241 attttcattg atggtcaaaa atccagggat tccctacagt catcctttag tagcaaaagc
    27301 ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag tagcggaatg
    27361 cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga tgacaggact
    27421 actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata ttgggtatcc
    27481 agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg aattgtctag
    27541 cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta ccaatatcta
    27601 tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta aatggaacct
    27661 tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt cagaactaca
    27721 agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag aagtgttgga
    27781 agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa taatggaaat
    27841 atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg ctatttctgt
    27901 agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt ttttccatgg
    27961 cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt ataatgattc
    28021 aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt
    28081 cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa ttccttcatt
    28141 aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg
    28201 aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag cagttccttt
    28261 agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga atgccagctg
    28321 ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg tgaaccgatt
    28381 aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca ggcggaggaa
    28441 cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact caagaaccga
    28501 atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc gtccctgata
    28561 caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt tccatgcata
    28621 acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg
    28681 attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg gtagtgtacg
    28741 ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta ccaggtgtta
    28801 ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa gatgcggctt
    28861 ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa gaagtagcag
    28921 atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag aaaacagtct
    28981 tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca gtttttttaa
    29041 gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga tattatgaag
    29101 agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct tatatcaaaa
    29161 atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt gcaacttcga
    29221 tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca tatgttacga
    29281 atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca aaaatgattg
    29341 cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc atgggtgatg
    29401 aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct gatgcttcaa
    29461 aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag
    29521 taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca atttgactcc
    29581 ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc aagagccaaa
    29641 aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa aaacggttca
    29701 agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg aagagatcaa
    29761 gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc ccaatttaaa
    29821 aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg tattgggtat
    29881 accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag cagttgcagt
    29941 atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat tgtcattagg
    30001 agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc ttaaaaaagc
    30061 acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat tcgatttgga
    30121 tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact acccagtgat
    30181 tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc tccctatctt
    30241 ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca atcagttatc
    30301 atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa gcaataaaaa
    30361 tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca ataatcttgc
    30421 gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga
    30481 catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg aagaatcggg
    30541 ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg aatcaagtga
    30601 agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg gatcatctga
    30661 ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa ctccgtcaag
    30721 tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct aaaaaccagt
    30781 aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt ttaaaacgga
    30841 cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt catggcaaaa
    30901 acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt cgtggctgaa
    30961 tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga agggattaac
    31021 cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc agtacgacaa
    31081 gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg
    31141 cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa ggaaatcacc
    31201 gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc tcctgaacgt
    31261 caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg aatcaaagat
    31321 cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac aggacctaaa
    31381 acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg
    31441 gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa agattttggt
    31501 acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca tgataagcaa
    31561 ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga tatctctatt
    31621 gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg ggatgcatac
    31681 ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca gtcagaacca
    31741 gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga acagattttc
    31801 aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg tggcgtcgta
    31861 ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga aatcttcggc
    31921 gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt atttacaaat
    31981 gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt
    32041 gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga aacaacaaac
    32101 acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga accaaaagag
    32161 tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt tgattaattc
    32221 atcctgggag gaataagata tggaattctc aattgataat aatatcaatg acggcgcagt
    32281 aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga
    32341 aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat taaaaaattc
    32401 aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag gtgctggttc
    32461 tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac gcgaagcttt
    32521 agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc
    32581 tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac
    32641 acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg
    32701 tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat tagaagttgt
    32761 agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt tacgacaagg
    32821 tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg actttgctga
    32881 cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg tggctagtgg
    32941 agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc ttgaaacatc
    33001 tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata tgacattgtt
    33061 cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg taaatatcat
    33121 tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac
    33181 aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac aagcacaaat
    33241 gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa cttcttcaga
    33301 agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc gtcctagagt
    33361 tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca accgcgaaga
    33421 aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc gtaaaaaata
    33481 aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa gaaattcagg
    33541 attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct gtaacaaaat
    33601 ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa
    33661 accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct gaaataaagt
    33721 ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt
    33781 actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg ttagagaaag
    33841 aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat ggaatttcac
    33901 cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc aaggttgttg
    33961 ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa gtgtttggta
    34021 cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca ccatgtagtg
    34081 aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt gcaacattta
    34141 tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat gccgctttta
    34201 aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta cgaataccaa
    34261 gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac aacacagaat
    34321 caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca
    34381 cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat tgaactacat
    34441 caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc tacacaacct
    34501 aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc tgaagctatg
    34561 gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca tttagtagaa
    34621 gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc actagatgga
    34681 gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat ggagattgac
    34741 agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga ggaggaacta
    34801 cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat ttctatagca
    34861 ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa tcagggctag
    34921 gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga
    34981 ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac ttagccgtac
    35041 aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga ttgaagcaac
    35101 aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat gtataccagc
    35161 actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc
    35221 aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag
    35281 aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg
    35341 cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag gaagattttg
    35401 aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa
    35461 tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg
    35521 acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag
    35581 taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta
    35641 tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg
    35701 tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta
    35761 aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata
    35821 ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa
    35881 aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact
    35941 catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata
    36001 taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac
    36061 tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt
    36121 caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa
    36181 agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc
    36241 agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga
    36301 aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac
    36361 tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc
    36421 tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta
    36481 cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa
    36541 tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac
    36601 acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc
    36661 actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg
    36721 aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct
    36781 cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg
    36841 gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt
    36901 gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg
    36961 tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta
    37021 ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc
    37081 atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat
    37141 cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca
    37201 tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtccat
    37261 ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc
    37321 agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc
    37381 ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta
    37441 caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga
    37501 aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg
    37561 caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt
    37621 gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga
    37681 tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg
    37741 ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag
    37801 tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc
    37861 tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag
    37921 caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc
    37981 acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa
    38041 agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc acgattggcg
    38101 gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt
    38161 ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac
    38221 acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg
    38281 gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac
    38341 aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga
    38401 agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac
    38461 aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt
    38521 acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata
    38581 tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata
    38641 caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc
    38701 tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg
    38761 cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag
    38821 tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac
    38881 agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa
    38941 atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa
    39001 atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc
    39061 attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg
    39121 tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc
    39181 tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt
    39241 tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac
    39301 ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa
    39361 cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt
    39421 tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga
    39481 ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga
    39541 tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg
    39601 taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt
    39661 tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg
    39721 gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt
    39781 cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga
    39841 aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt
    39901 cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag
    39961 agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc
    40021 tattttcaac catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc
    40081 cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga
    40141 tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga
    40201 atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac
    40261 gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt
    40321 tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc
    40381 ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta
    40441 acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga
    40501 tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa
    40561 tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt
    40621 ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt
    40681 gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc
    40741 ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt
    40801 tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga
    40861 agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt
    40921 gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc
    40981 ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat
    41041 agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg
    41101 tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta
    41161 cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt
    41221 tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag
    41281 acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct
    41341 gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc
    41401 aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc
    41461 tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt
    41521 ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca
    41581 atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg
    41641 ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa
    41701 aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc
    41761 agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga
    41821 aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt
    41881 gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg
    41941 tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa
    42001 acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa
    42061 aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt
    42121 gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag
    42181 agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg
    42241 atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta
    42301 atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg
    42361 atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact
    42421 tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact
    42481 cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat
    42541 aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat
    42601 gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag
    42661 ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa
    42721 taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg
    42781 aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg
    42841 taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg
    42901 atggtatttt agacggcacg atccttttac caggattaac tggtccattt acggtataca
    42961 agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac
    43021 aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc
    43081 ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag
    43141 atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga
    43201 tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag
    43261 ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca
    43321 ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa
    43381 tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt
    43441 taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg
    43501 aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca
    43561 atattactca tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag
    43621 ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc
    43681 caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag
    43741 cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag
    43801 acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat
    43861 tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt
    43921 taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt
    43981 atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg
    44041 agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga
    44101 tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat
    44161 ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa
    44221 tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg
    44281 gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt
    44341 taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga
    44401 atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc
    44461 accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc
    44521 ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt
    44581 ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca
    44641 agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt
    44701 gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat
    44761 tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt
    44821 gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat
    44881 tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc
    44941 agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt
    45001 gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg
    45061 aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt
    45121 ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc
    45181 ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt
    45241 caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat
    45301 tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca
    45361 tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga
    45421 taataatcta agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat
    45481 ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc
    45541 agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa
    45601 agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag
    45661 gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag
    45721 aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa
    45781 caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc
    45841 cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc
    45901 gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg
    45961 caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag
    46021 gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact
    46081 ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg
    46141 ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa
    46201 ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat
    46261 agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt
    46321 ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg
    46381 cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa
    46441 tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg
    46501 aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa
    46561 gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg
    46621 ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca
    46681 aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa
    46741 ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac
    46801 cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc
    46861 tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact
    46921 tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg
    46981 gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt
    47041 ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta
    47101 gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg
    47161 gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat
    47221 tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa
    47281 gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga
    47341 gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag
    47401 actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct
    47461 tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc
    47521 aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta
    47581 aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg
    47641 acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa
    47701 gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg
    47761 gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa
    47821 ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa
    47881 ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg
    47941 gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca
    48001 tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct
    48061 gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc
    48121 ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat
    48181 ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg
    48241 ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca
    48301 aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa
    48361 tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat
    48421 aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac
    48481 tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc
    48541 ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac
    48601 aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa
    48661 agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga
    48721 taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat
    48781 gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat
    48841 gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa
    48901 ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc
    48961 atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg
    49021 gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca
    49081 cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat
    49141 atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt
    49201 actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa
    49261 cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga
    49321 gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt
    49381 ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg
    49441 ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa
    49501 tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt
    49561 tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg
    49621 tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc
    49681 ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga
    49741 ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa
    49801 atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt
    49861 cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag
    49921 ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta
    49981 cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac
    50041 cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg
    50101 atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc ctaggattat
    50161 ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg
    50221 gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac
    50281 taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt
    50341 tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg
    50401 catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta
    50461 aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca
    50521 acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct
    50581 tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg
    50641 ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa
    50701 caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga
    50761 tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat
    50821 aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttctagga
    50881 ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta
    50941 gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg
    51001 ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc
    51061 aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt
    51121 taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct
    51181 ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc
    51241 acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa
    51301 gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt
    51361 ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat
    51421 cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc
    51481 gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga
    51541 caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg
    51601 aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc
    51661 ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga
    51721 tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta
    51781 agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt
    51841 tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga
    51901 ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga
    51961 acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg
    52021 gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct
    52081 aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa
    52141 tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct
    52201 ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat
    52261 cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa
    52321 gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga
    52381 aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa
    52441 aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt
    52501 ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc
    52561 tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata
    52621 ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt
    52681 aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta
    52741 tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa
    52801 atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt
    52861 agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt
    52921 agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata
    52981 tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg
    53041 ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt
    53101 gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt
    53161 gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt
    53221 tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga
    53281 aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg
    53341 aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac
    53401 aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg
    53461 cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat aacccctgta
    53521 tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa actagttatc
    53581 atttttatat aaactatcga acacagtatt gattttttct ttatcctgat cttcaagctc
    53641 tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
    53701 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgtg tatgccttag
    53761 tccatgcaaa gtaaattgct tttcaatccc taattctttt aattttttgc gcaaatattt
    53821 gctgacggcg tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa aattatgagg
    53881 gtttttgatt tcaaaattct cgaataattc tttttgattg attttaaact tttttaagag
    53941 atcaatcgta tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt ttgtttttcc
    54001 ccattcattt aatttataat tccatgtttt attaatagtg atgatttgat cttcgaaatc
    54061 gatatcttcc catgttaatc ctagaagttc agcaaatcgc attccagtgg caccagcaac
    54121 taacacgagc ataggagaag agtactgcgc agaaaggttt tcttctacga gtttcattaa
    54181 agatttaaat tgatcataat ctaaatattt atcttctttt ggcttcaatg aatcttttcc
    54241 tttgattact gcttttctag ttggatcaaa cggaattaat ccttcttcta cggcatcttt
    54301 caacgacgct ctaatatggt tattaaactt aataacggta gattttacat ggtctttagc
    54361 atacttattt aaaaagcgtt gatatcccac tctatctaaa tcagaaatca ataccgctgg
    54421 catatatttc tttatgttca ttaacgtatc ttcgtatttt ctataagtga taggagatac
    54481 tgttccttct ttataaagtt gcatccaatc ctcaaagtaa tctgataata atagattttt
    54541 gcgatcccct ttaaggcctt tgttcaagtt atattctaat tcgttggcgg catcttttgc
    54601 ttctcctttt gtcttaaatc ctgattttct tatcttcgtg tattttccgt catctttttt
    54661 gtatgaaatt tcgtattgcc aactatttcc acgtttgact aatcttgcca taattgaatc
    54721 agctctcttt ctctgataca ataggaacta taaagaagcc cattgtttgg gttttattat
    54781 tttttgcaca tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt tttaatggta
    54841 taattggatg tgtattagat catccaatca gaggtgttta ttatgattaa agtgattttt
    54901 aaaagaataa ctgaacttcc agagtttaga ttatttgttg attattggta tgtctggttt
    54961 tttattgttg cattgctgat aggtctagat gtcatattag aaagaaaact taagtaagag
    55021 ttgagttaat aaattgaatt cctaaatgag atattttgta gcccatattt tgtttgccga
    55081 catcacctaa tactatggag ctaatcgttt tttctaacag accaaaatca gataatctat
    55141 ttaagctacc atctattagc tcttgtgaat agccagacat gctgatattt ccagtgaaat
    55201 tttcgtttcc acgactcatt aagcatgcta acaattgaat ctcaatcgat gttaattgat
    55261 tcagtacaag aagataatac tcctcgttat cccatgaagc gctgttaatt cgaagtaaac
    55321 aattcttata aagattttta tacatatcta ttttattttg tgcctttgat ttttctacct
    55381 cgtcatttat cccttcgaat attccaatga gttggtcctt gttctctaag ttgaaaacat
    55441 cttctggaat ttgatcgcga atgttttcta atgattcgtt taaatttttg tagaatttct
    55501 cgatgcgttt gaaacgcttc tcgtttttag acccaaaata aagtgactgc acaacgccac
    55561 caatataagg aattgctcca ataccgcctt caactattaa ttcaagatat tcttttttgg
    55621 ttagtttttc attgctatta tccatagtta cctcctattt tgtcatttcc tttacaagca
    55681 ttgtgactcc tgatggcact cttttgtata ggttttatta ttgtgcacaa tctaactctt
    55741 agtggggcgt ggttcattta ttttttactt actttactat taatcatatc ccattgagca
    55801 taggattttt ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt tactttatcg
    55861 ccctttgtaa aggtatcagc attgtttaag tccttcgcaa aaattacata tgaaacttta
    55921 ggtgttgcag aaatatcatt ccaactttta tcatcgtact ctttgtctgc tataatggct
    55981 attctagtag tcattgattc gataacagtt ccttcaaaag tatatgtttt accactaata
    56041 tatttagtaa aagctgtatt ttgctcagta gctggagtaa tagaataata actatctaag
    56101 aaagcacccc agtcatgatt atttgtctct aaaaattctt caaattgagt taggctttta
    56161 ttgctgctgt tagatgtagt agtttgagta tcagatgatg agctttcctg tttgttattg
    56221 tcatttgcct cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat aatcccgata
    56281 ataaggagaa tcataggtgt tttcttattt gtttttttga taaatcttac aattaacatg
    56341 attaaaccta taataaataa aacaatacca gtaaaaccaa atataccgtc cattaataga
    56401 tcctctttct cttttgatat tgaatattat aaaccaagca attctttttt cttaaaatcg
    56461 aattcttctt gattaataat cccttcatct aacaactctt tatacttttt aatttcatct
    56521 gcact
//
LOCUS       DAJSVP010000010        56308 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSVP010000010 DAJSVP010000000
VERSION     DAJSVP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.010"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(71..1639)
                     /locus_tag="ON449_000601"
     CDS             complement(71..1639)
                     /locus_tag="ON449_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3933217.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1643..2053)
                     /locus_tag="ON449_000602"
     CDS             complement(1643..2053)
                     /locus_tag="ON449_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3933218.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2050..2973)
                     /locus_tag="ON449_000603"
     CDS             complement(2050..2973)
                     /locus_tag="ON449_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3933219.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2970..3848)
                     /locus_tag="ON449_000604"
     CDS             complement(2970..3848)
                     /locus_tag="ON449_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3933220.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3835..5172)
                     /locus_tag="ON449_000605"
     CDS             complement(3835..5172)
                     /locus_tag="ON449_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3933221.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5175..6278)
                     /locus_tag="ON449_000606"
     CDS             complement(5175..6278)
                     /locus_tag="ON449_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3933222.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6352..7860)
                     /locus_tag="ON449_000607"
     CDS             complement(6352..7860)
                     /locus_tag="ON449_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3933223.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7826..8518)
                     /locus_tag="ON449_000608"
     CDS             complement(7826..8518)
                     /locus_tag="ON449_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3933224.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8540..9514)
                     /locus_tag="ON449_000609"
     CDS             complement(8540..9514)
                     /locus_tag="ON449_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933225.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9814..10686
                     /locus_tag="ON449_000610"
     CDS             9814..10686
                     /locus_tag="ON449_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933226.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11144..13342)
                     /locus_tag="ON449_000611"
     CDS             complement(11144..13342)
                     /locus_tag="ON449_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933227.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13574..14566
                     /locus_tag="ON449_000612"
     CDS             13574..14566
                     /locus_tag="ON449_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933228.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14626..16047
                     /locus_tag="ON449_000613"
     CDS             14626..16047
                     /locus_tag="ON449_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3933229.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16040..17539
                     /locus_tag="ON449_000614"
     CDS             16040..17539
                     /locus_tag="ON449_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3933230.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17578..18972
                     /gene="uxaC"
                     /locus_tag="ON449_000615"
     CDS             17578..18972
                     /gene="uxaC"
                     /locus_tag="ON449_000615"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3933231.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19131..19814
                     /locus_tag="ON449_000616"
     CDS             19131..19814
                     /locus_tag="ON449_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3933232.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19816..20652
                     /locus_tag="ON449_000617"
     CDS             19816..20652
                     /locus_tag="ON449_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3933233.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20663..22417
                     /locus_tag="ON449_000618"
     CDS             20663..22417
                     /locus_tag="ON449_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933234.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22693..23034)
                     /locus_tag="ON449_000619"
     CDS             complement(22693..23034)
                     /locus_tag="ON449_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3933235.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23102..25273)
                     /locus_tag="ON449_000620"
     CDS             complement(23102..25273)
                     /locus_tag="ON449_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3933236.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25442..26341
                     /locus_tag="ON449_000621"
     CDS             25442..26341
                     /locus_tag="ON449_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3933237.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26412..27269)
                     /locus_tag="ON449_000622"
     CDS             complement(26412..27269)
                     /locus_tag="ON449_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3933238.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27254..29956)
                     /locus_tag="ON449_000623"
     CDS             complement(27254..29956)
                     /locus_tag="ON449_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3933239.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29969..31258)
                     /locus_tag="ON449_000624"
     CDS             complement(29969..31258)
                     /locus_tag="ON449_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3933240.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31408..32454
                     /locus_tag="ON449_000625"
     CDS             31408..32454
                     /locus_tag="ON449_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933241.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32456..34609
                     /locus_tag="ON449_000626"
     CDS             32456..34609
                     /locus_tag="ON449_000626"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3933242.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35082..36533)
                     /locus_tag="ON449_000627"
     CDS             complement(35082..36533)
                     /locus_tag="ON449_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3933243.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36530..38254)
                     /locus_tag="ON449_000628"
     CDS             complement(36530..38254)
                     /locus_tag="ON449_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3933244.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38247..38861)
                     /locus_tag="ON449_000629"
     CDS             complement(38247..38861)
                     /locus_tag="ON449_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3933245.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39206..40663)
                     /locus_tag="ON449_000630"
     CDS             complement(39206..40663)
                     /locus_tag="ON449_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3933246.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40704..41624)
                     /locus_tag="ON449_000631"
     CDS             complement(40704..41624)
                     /locus_tag="ON449_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933247.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41636..42583)
                     /locus_tag="ON449_000632"
     CDS             complement(41636..42583)
                     /locus_tag="ON449_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933248.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(43062..43781)
                     /locus_tag="ON449_000633"
     CDS             complement(43062..43781)
                     /locus_tag="ON449_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3933249.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43830..45476)
                     /locus_tag="ON449_000634"
     CDS             complement(43830..45476)
                     /locus_tag="ON449_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3933250.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45655..46245
                     /locus_tag="ON449_000635"
     CDS             45655..46245
                     /locus_tag="ON449_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3933251.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46337..47842
                     /locus_tag="ON449_000636"
     CDS             46337..47842
                     /locus_tag="ON449_000636"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3933252.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(47926..49278)
                     /gene="celB"
                     /locus_tag="ON449_000637"
     CDS             complement(47926..49278)
                     /gene="celB"
                     /locus_tag="ON449_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3933253.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49278..49796)
                     /locus_tag="ON449_000638"
     CDS             complement(49278..49796)
                     /locus_tag="ON449_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933254.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49812..50138)
                     /locus_tag="ON449_000639"
     CDS             complement(49812..50138)
                     /locus_tag="ON449_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3933255.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50151..52130)
                     /locus_tag="ON449_000640"
     CDS             complement(50151..52130)
                     /locus_tag="ON449_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3933256.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52151..52465)
                     /locus_tag="ON449_000641"
     CDS             complement(52151..52465)
                     /locus_tag="ON449_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3933257.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52633..52926
                     /locus_tag="ON449_000642"
     CDS             52633..52926
                     /locus_tag="ON449_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3933258.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(53004..54116)
                     /locus_tag="ON449_000643"
     CDS             complement(53004..54116)
                     /locus_tag="ON449_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3933259.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54503..54682
                     /locus_tag="ON449_000644"
     CDS             54503..54682
                     /locus_tag="ON449_000644"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933260.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            54876..55046
                     /locus_tag="ON449_000645"
     CDS             54876..55046
                     /locus_tag="ON449_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3933261.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(55470..>56308)
                     /locus_tag="ON449_000646"
     CDS             complement(55470..>56308)
                     /locus_tag="ON449_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3933262.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        16788 a        11517 c         9919 g        18084 t
ORIGIN      
        1 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
       61 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
      121 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
      181 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
      241 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
      301 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
      361 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
      421 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
      481 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
      541 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
      601 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
      661 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
      721 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
      781 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
      841 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
      901 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
      961 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     1021 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     1081 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     1141 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     1201 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     1261 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     1321 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     1381 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     1441 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     1501 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     1561 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     1621 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     1681 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     1741 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     1801 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     1861 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     1921 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     1981 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     2041 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     2101 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     2161 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     2221 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     2281 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     2341 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     2401 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     2461 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     2521 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     2581 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     2641 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     2701 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     2761 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     2821 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     2881 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     2941 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     3001 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     3061 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     3121 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     3181 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     3241 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     3301 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     3361 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     3421 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     3481 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     3541 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     3601 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     3661 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     3721 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     3781 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     3841 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     3901 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     3961 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     4021 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     4081 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     4141 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     4201 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     4261 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     4321 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     4381 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     4441 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     4501 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     4561 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     4621 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     4681 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     4741 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     4801 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     4861 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     4921 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     4981 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     5041 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     5101 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     5161 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     5221 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     5281 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     5341 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     5401 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     5461 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     5521 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     5581 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     5641 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     5701 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     5761 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     5821 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     5881 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     5941 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     6001 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     6061 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     6121 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     6181 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     6241 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     6301 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     6361 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     6421 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     6481 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     6541 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     6601 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
     6661 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
     6721 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
     6781 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
     6841 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
     6901 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
     6961 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
     7021 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
     7081 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
     7141 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
     7201 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
     7261 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
     7321 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
     7381 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
     7441 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
     7501 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
     7561 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
     7621 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
     7681 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
     7741 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
     7801 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
     7861 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
     7921 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
     7981 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
     8041 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
     8101 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
     8161 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
     8221 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
     8281 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
     8341 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
     8401 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
     8461 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
     8521 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
     8581 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
     8641 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
     8701 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
     8761 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
     8821 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
     8881 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
     8941 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
     9001 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
     9061 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
     9121 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
     9181 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
     9241 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
     9301 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
     9361 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
     9421 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
     9481 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
     9541 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
     9601 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
     9661 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
     9721 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
     9781 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
     9841 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
     9901 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
     9961 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    10021 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    10081 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    10141 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    10201 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    10261 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    10321 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    10381 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    10441 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    10501 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    10561 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    10621 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    10681 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    10741 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    10801 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    10861 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    10921 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    10981 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    11041 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    11101 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    11161 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    11221 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    11281 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    11341 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    11401 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    11461 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    11521 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    11581 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    11641 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    11701 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    11761 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    11821 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    11881 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    11941 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    12001 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    12061 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    12121 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    12181 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    12241 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    12301 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    12361 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    12421 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    12481 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    12541 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    12601 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    12661 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    12721 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    12781 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    12841 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    12901 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    12961 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    13021 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    13081 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    13141 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    13201 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    13261 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    13321 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    13381 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    13441 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    13501 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    13561 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    13621 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    13681 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    13741 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    13801 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    13861 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    13921 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    13981 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    14041 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    14101 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    14161 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    14221 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    14281 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    14341 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    14401 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    14461 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    14521 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    14581 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    14641 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    14701 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    14761 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    14821 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    14881 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    14941 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    15001 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    15061 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    15121 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    15181 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    15241 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    15301 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    15361 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    15421 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    15481 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    15541 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    15601 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    15661 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    15721 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    15781 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    15841 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    15901 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    15961 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    16021 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    16081 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    16141 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    16201 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    16261 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    16321 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    16381 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    16441 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    16501 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    16561 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    16621 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    16681 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    16741 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    16801 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    16861 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    16921 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    16981 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    17041 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    17101 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    17161 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    17221 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    17281 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    17341 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    17401 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    17461 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    17521 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    17581 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    17641 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    17701 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    17761 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    17821 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    17881 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    17941 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    18001 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    18061 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    18121 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    18181 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    18241 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    18301 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    18361 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    18421 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    18481 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    18541 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    18601 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    18661 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    18721 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    18781 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    18841 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    18901 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    18961 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    19021 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    19081 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    19141 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    19201 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    19261 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    19321 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    19381 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    19441 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    19501 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    19561 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    19621 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    19681 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    19741 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    19801 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    19861 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    19921 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    19981 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    20041 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    20101 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    20161 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    20221 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    20281 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    20341 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    20401 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    20461 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    20521 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    20581 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    20641 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    20701 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    20761 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    20821 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    20881 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    20941 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    21001 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    21061 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    21121 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    21181 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    21241 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    21301 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    21361 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    21421 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    21481 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    21541 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    21601 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    21661 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    21721 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    21781 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    21841 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    21901 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    21961 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    22021 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    22081 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    22141 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    22201 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    22261 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    22321 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    22381 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    22441 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    22501 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    22561 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    22621 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    22681 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    22741 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    22801 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    22861 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    22921 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    22981 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    23041 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    23101 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    23161 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    23221 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    23281 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    23341 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    23401 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    23461 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    23521 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    23581 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    23641 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    23701 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    23761 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    23821 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    23881 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    23941 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    24001 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    24061 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    24121 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    24181 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    24241 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    24301 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    24361 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    24421 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    24481 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    24541 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    24601 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    24661 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    24721 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    24781 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    24841 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    24901 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    24961 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    25021 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    25081 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    25141 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    25201 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    25261 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    25321 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    25381 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    25441 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    25501 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    25561 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    25621 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    25681 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    25741 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    25801 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    25861 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    25921 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    25981 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    26041 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    26101 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    26161 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    26221 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    26281 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    26341 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    26401 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    26461 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    26521 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    26581 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    26641 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    26701 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    26761 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    26821 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    26881 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    26941 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    27001 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    27061 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    27121 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    27181 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    27241 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    27301 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    27361 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    27421 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    27481 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    27541 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    27601 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    27661 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    27721 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    27781 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    27841 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    27901 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    27961 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    28021 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    28081 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    28141 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    28201 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    28261 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    28321 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    28381 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    28441 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    28501 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    28561 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    28621 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    28681 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    28741 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    28801 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    28861 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    28921 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    28981 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    29041 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    29101 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    29161 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    29221 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    29281 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    29341 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    29401 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    29461 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    29521 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    29581 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    29641 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    29701 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    29761 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    29821 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    29881 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    29941 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    30001 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    30061 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    30121 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    30181 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    30241 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    30301 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    30361 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    30421 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    30481 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    30541 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    30601 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    30661 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    30721 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    30781 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    30841 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    30901 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    30961 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    31021 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    31081 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    31141 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    31201 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    31261 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    31321 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    31381 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    31441 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    31501 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    31561 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    31621 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    31681 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    31741 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    31801 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    31861 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    31921 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    31981 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    32041 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    32101 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    32161 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    32221 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    32281 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    32341 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    32401 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    32461 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    32521 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    32581 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    32641 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    32701 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    32761 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    32821 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    32881 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    32941 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    33001 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    33061 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    33121 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    33181 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    33241 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    33301 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    33361 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    33421 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    33481 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    33541 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    33601 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    33661 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    33721 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    33781 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    33841 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    33901 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    33961 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    34021 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    34081 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    34141 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    34201 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    34261 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    34321 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    34381 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    34441 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    34501 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    34561 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    34621 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    34681 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    34741 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    34801 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    34861 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    34921 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    34981 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    35041 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    35101 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    35161 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    35221 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    35281 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    35341 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    35401 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    35461 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    35521 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    35581 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    35641 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    35701 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    35761 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    35821 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    35881 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    35941 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    36001 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    36061 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    36121 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    36181 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    36241 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    36301 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    36361 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    36421 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    36481 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    36541 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    36601 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    36661 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    36721 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    36781 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    36841 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    36901 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    36961 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    37021 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    37081 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    37141 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    37201 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    37261 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    37321 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    37381 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    37441 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    37501 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    37561 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    37621 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    37681 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    37741 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    37801 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    37861 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    37921 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    37981 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    38041 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    38101 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    38161 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    38221 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    38281 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    38341 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    38401 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    38461 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    38521 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    38581 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    38641 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    38701 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    38761 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    38821 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    38881 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    38941 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    39001 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    39061 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    39121 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    39181 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    39241 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    39301 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    39361 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    39421 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    39481 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    39541 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    39601 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    39661 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    39721 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    39781 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    39841 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    39901 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    39961 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    40021 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    40081 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    40141 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    40201 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    40261 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    40321 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    40381 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    40441 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    40501 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    40561 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    40621 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    40681 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    40741 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    40801 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    40861 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    40921 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    40981 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    41041 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    41101 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    41161 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    41221 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    41281 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    41341 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    41401 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    41461 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    41521 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    41581 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    41641 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    41701 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    41761 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    41821 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    41881 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    41941 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    42001 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    42061 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    42121 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    42181 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    42241 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    42301 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    42361 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    42421 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    42481 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    42541 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    42601 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    42661 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    42721 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    42781 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    42841 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    42901 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    42961 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    43021 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    43081 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    43141 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    43201 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    43261 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    43321 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    43381 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    43441 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    43501 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    43561 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    43621 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    43681 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    43741 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    43801 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    43861 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    43921 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    43981 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    44041 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    44101 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    44161 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    44221 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    44281 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    44341 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    44401 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    44461 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    44521 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    44581 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    44641 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    44701 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    44761 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    44821 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    44881 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    44941 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    45001 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    45061 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    45121 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    45181 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    45241 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    45301 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    45361 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    45421 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    45481 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    45541 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    45601 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    45661 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    45721 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    45781 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    45841 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    45901 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    45961 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    46021 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    46081 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    46141 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    46201 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    46261 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    46321 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    46381 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    46441 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    46501 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    46561 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    46621 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    46681 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    46741 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    46801 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    46861 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    46921 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    46981 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    47041 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    47101 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    47161 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    47221 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    47281 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    47341 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    47401 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    47461 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    47521 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    47581 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    47641 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    47701 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    47761 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    47821 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    47881 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    47941 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    48001 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    48061 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    48121 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    48181 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    48241 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    48301 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    48361 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    48421 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    48481 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    48541 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    48601 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    48661 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    48721 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    48781 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    48841 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    48901 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    48961 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    49021 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    49081 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    49141 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    49201 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    49261 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    49321 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    49381 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    49441 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    49501 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    49561 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    49621 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    49681 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    49741 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    49801 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    49861 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    49921 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    49981 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    50041 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    50101 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    50161 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    50221 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    50281 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    50341 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    50401 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    50461 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    50521 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    50581 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    50641 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    50701 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    50761 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    50821 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    50881 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    50941 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    51001 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    51061 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    51121 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    51181 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    51241 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    51301 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    51361 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    51421 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    51481 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    51541 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    51601 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    51661 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    51721 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    51781 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    51841 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    51901 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    51961 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    52021 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    52081 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    52141 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    52201 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    52261 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    52321 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    52381 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    52441 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    52501 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    52561 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    52621 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    52681 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    52741 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    52801 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    52861 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    52921 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    52981 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    53041 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    53101 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    53161 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    53221 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    53281 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    53341 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    53401 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    53461 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    53521 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    53581 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    53641 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    53701 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    53761 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    53821 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    53881 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    53941 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    54001 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    54061 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    54121 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    54181 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    54241 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    54301 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    54361 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    54421 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    54481 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    54541 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    54601 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    54661 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    54721 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    54781 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    54841 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    54901 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    54961 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    55021 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    55081 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    55141 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    55201 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    55261 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    55321 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    55381 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    55441 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    55501 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    55561 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    55621 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    55681 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    55741 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    55801 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    55861 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    55921 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    55981 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    56041 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    56101 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    56161 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    56221 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt
    56281 ggcgcttgag tttctaccag ctgatatg
//
LOCUS       DAJSVP010000011        54214 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSVP010000011 DAJSVP010000000
VERSION     DAJSVP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.011"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            74..238
                     /locus_tag="ON449_000647"
                     /pseudo
     CDS             74..238
                     /locus_tag="ON449_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            486..728
                     /locus_tag="ON449_000648"
     CDS             486..728
                     /locus_tag="ON449_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933263.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(833..2041)
                     /locus_tag="ON449_000649"
     CDS             complement(833..2041)
                     /locus_tag="ON449_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HCR3933264.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2331..3296
                     /locus_tag="ON449_000650"
     CDS             2331..3296
                     /locus_tag="ON449_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933265.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3318..3530
                     /locus_tag="ON449_000651"
     CDS             3318..3530
                     /locus_tag="ON449_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3933266.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3605..4081
                     /locus_tag="ON449_000652"
     CDS             3605..4081
                     /locus_tag="ON449_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCR3933267.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4078..4577
                     /locus_tag="ON449_000653"
                     /pseudo
     CDS             4078..4577
                     /locus_tag="ON449_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            5137..6954
                     /locus_tag="ON449_000654"
     CDS             5137..6954
                     /locus_tag="ON449_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3933268.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7060..8388
                     /locus_tag="ON449_000655"
                     /pseudo
     CDS             7060..8388
                     /locus_tag="ON449_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            8465..8626
                     /locus_tag="ON449_000656"
     CDS             8465..8626
                     /locus_tag="ON449_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HCR3933269.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8667..9047
                     /locus_tag="ON449_000657"
     CDS             8667..9047
                     /locus_tag="ON449_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HCR3933270.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9062..9343
                     /locus_tag="ON449_000658"
     CDS             9062..9343
                     /locus_tag="ON449_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933271.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9602..10384
                     /locus_tag="ON449_000659"
     CDS             9602..10384
                     /locus_tag="ON449_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR3933272.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10557..12275
                     /locus_tag="ON449_000660"
     CDS             10557..12275
                     /locus_tag="ON449_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR3933273.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12277..13131
                     /locus_tag="ON449_000661"
     CDS             12277..13131
                     /locus_tag="ON449_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3933274.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13807..14127
                     /locus_tag="ON449_000662"
     CDS             13807..14127
                     /locus_tag="ON449_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933275.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14151..14561
                     /locus_tag="ON449_000663"
     CDS             14151..14561
                     /locus_tag="ON449_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3933276.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14708..14914
                     /locus_tag="ON449_000664"
     CDS             14708..14914
                     /locus_tag="ON449_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3933277.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15261..15782
                     /locus_tag="ON449_000665"
     CDS             15261..15782
                     /locus_tag="ON449_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933278.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15840..16265
                     /locus_tag="ON449_000666"
     CDS             15840..16265
                     /locus_tag="ON449_000666"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3933279.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16444..16674
                     /locus_tag="ON449_000667"
     CDS             16444..16674
                     /locus_tag="ON449_000667"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCR3933280.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16708..16890
                     /locus_tag="ON449_000668"
     CDS             16708..16890
                     /locus_tag="ON449_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HCR3933281.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            17003..18514
                     /locus_tag="ON449_000669"
     CDS             17003..18514
                     /locus_tag="ON449_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933282.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18837..19061
                     /locus_tag="ON449_000670"
     CDS             18837..19061
                     /locus_tag="ON449_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HCR3933283.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19054..19620
                     /locus_tag="ON449_000671"
     CDS             19054..19620
                     /locus_tag="ON449_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HCR3933284.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19608..19838
                     /locus_tag="ON449_000672"
     CDS             19608..19838
                     /locus_tag="ON449_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933285.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            19988..20404
                     /locus_tag="ON449_000673"
     CDS             19988..20404
                     /locus_tag="ON449_000673"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HCR3933286.1"
                     /translation="MNKPVLFVYRKKKEYKKMSRAKNPKYESMSLEELKANMEKSRQE
                     LEAAIHHKNILEQRKKLVERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQN
                     SSYNKSIVRQAHIAALHKDQQQIAEAVKKTEKGDDS"
     gene            20404..22131
                     /locus_tag="ON449_000674"
     CDS             20404..22131
                     /locus_tag="ON449_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR3933287.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22161..24077
                     /locus_tag="ON449_000675"
     CDS             22161..24077
                     /locus_tag="ON449_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933288.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24762..24944
                     /locus_tag="ON449_000676"
     CDS             24762..24944
                     /locus_tag="ON449_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933289.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            24971..26668
                     /locus_tag="ON449_000677"
     CDS             24971..26668
                     /locus_tag="ON449_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3933290.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26693..26824
                     /locus_tag="ON449_000678"
     CDS             26693..26824
                     /locus_tag="ON449_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933291.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26916..27779
                     /locus_tag="ON449_000679"
     CDS             26916..27779
                     /locus_tag="ON449_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933292.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27816..28352
                     /locus_tag="ON449_000680"
     CDS             27816..28352
                     /locus_tag="ON449_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCR3933293.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            28366..28788
                     /locus_tag="ON449_000681"
     CDS             28366..28788
                     /locus_tag="ON449_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3933294.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28718..31117
                     /locus_tag="ON449_000682"
     CDS             28718..31117
                     /locus_tag="ON449_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3933295.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31149..33140
                     /locus_tag="ON449_000683"
     CDS             31149..33140
                     /locus_tag="ON449_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3933296.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33163..33414
                     /locus_tag="ON449_000684"
     CDS             33163..33414
                     /locus_tag="ON449_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCR3933297.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33404..34633
                     /locus_tag="ON449_000685"
     CDS             33404..34633
                     /locus_tag="ON449_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCR3933298.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34630..36711
                     /locus_tag="ON449_000686"
     CDS             34630..36711
                     /locus_tag="ON449_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR3933299.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36860..40780
                     /locus_tag="ON449_000687"
     CDS             36860..40780
                     /locus_tag="ON449_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCR3933300.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40781..41725
                     /locus_tag="ON449_000688"
     CDS             40781..41725
                     /locus_tag="ON449_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933301.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41729..41932
                     /locus_tag="ON449_000689"
     CDS             41729..41932
                     /locus_tag="ON449_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCR3933302.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42232..42468)
                     /locus_tag="ON449_000690"
     CDS             complement(42232..42468)
                     /locus_tag="ON449_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933303.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42651..43037)
                     /locus_tag="ON449_000691"
     CDS             complement(42651..43037)
                     /locus_tag="ON449_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCR3933304.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(43019..44347)
                     /locus_tag="ON449_000692"
     CDS             complement(43019..44347)
                     /locus_tag="ON449_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3933305.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44308..44637)
                     /gene="mobC"
                     /locus_tag="ON449_000693"
     CDS             complement(44308..44637)
                     /gene="mobC"
                     /locus_tag="ON449_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR3933306.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44895..45266)
                     /locus_tag="ON449_000694"
     CDS             complement(44895..45266)
                     /locus_tag="ON449_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3933307.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45411..45596
                     /locus_tag="ON449_000695"
     CDS             45411..45596
                     /locus_tag="ON449_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3933308.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            45754..45921
                     /locus_tag="ON449_000696"
     CDS             45754..45921
                     /locus_tag="ON449_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933309.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            46539..47198
                     /gene="vanR"
                     /locus_tag="ON449_000697"
     CDS             46539..47198
                     /gene="vanR"
                     /locus_tag="ON449_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCR3933310.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47201..48544
                     /gene="vanS"
                     /locus_tag="ON449_000698"
     CDS             47201..48544
                     /gene="vanS"
                     /locus_tag="ON449_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3933311.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48715..49521
                     /locus_tag="ON449_000699"
     CDS             48715..49521
                     /locus_tag="ON449_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HCR3933312.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            49539..50366
                     /gene="vanW"
                     /locus_tag="ON449_000700"
     CDS             49539..50366
                     /gene="vanW"
                     /locus_tag="ON449_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCR3933313.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50363..51334
                     /locus_tag="ON449_000701"
     CDS             50363..51334
                     /locus_tag="ON449_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCR3933314.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51327..52355
                     /gene="vanB"
                     /locus_tag="ON449_000702"
     CDS             51327..52355
                     /gene="vanB"
                     /locus_tag="ON449_000702"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCR3933315.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52361..52969
                     /locus_tag="ON449_000703"
     CDS             52361..52969
                     /locus_tag="ON449_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCR3933316.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(53035..53208)
                     /locus_tag="ON449_000704"
     CDS             complement(53035..53208)
                     /locus_tag="ON449_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933317.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(53327..>54214)
                     /locus_tag="ON449_000705"
     CDS             complement(53327..>54214)
                     /locus_tag="ON449_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3933318.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT        15518 a        12011 c        14077 g        12608 t
ORIGIN      
        1 aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc cttatattat
       61 tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg tacgaccgaa
      121 aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt caggaaaaaa
      181 aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt cctcaaaatt
      241 ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga gaactcaatt
      301 tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata ctgctaatac
      361 ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg cggcgttcct
      421 ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag aaaggaggcg
      481 acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg caggaaaagc
      541 ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc
      601 gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt ccctatcagc
      661 cgggcaagct gaccgactat gcagcggtca aattcacagt gaaagacctg ctaataaaag
      721 tcaagtagaa atttcagatt tttagggagg cgaaaaaatg caatacaaaa tcaagccgct
      781 gagcatctca aaattaaaac ggcggccagc cagatggtat agagtgtaaa aatcagtcgt
      841 gctccaggga ttccaagtag gctttcacag aagcgtagta gatgcgcagg aacttgttgg
      901 cagaggccat catgtagaca cgatatggct tgccttcaga gcgtttcttg tccatgaact
      961 ggtacaccgg ctcgtctatt ggagcacatt gcaggatgac gctcatcacc agaaagagcg
     1021 tcctgcgcag tgaggcagat cctcgcttgg aaatgctacg gctacggaca tctatttgac
     1081 cggattggta gggcggggcg tcaatacccg caaaggcaac caacgctttt ttggaatgaa
     1141 aacggcgtac atcgccaatt tcagccagga gttgagggcc gagggttggg ccaacaccaa
     1201 acatccccat cactacagga tactccggca gagaagctgc cagggattgc atctcctgtt
     1261 tgagagcagc taacgcggaa gaagttgtct ggagttgaga aatggcctgt tctaccaaaa
     1321 gttttgccgt atccgttttc ggtatgacac cgaagtgtct gcaagcagag gcataaatat
     1381 ccagcgcctt atcctcgctg aaattgtagc cgtgctttct gcaccacttc tggtattggg
     1441 tagtaaaggc cttctcagac cggccacaaa cgcactcgca atgccaaaaa gtagcgacaa
     1501 agtccaccca cttctcgctg ccatcggcgc ggggcggact ggtaaacaag cgatttgcgt
     1561 ctgggaaggt ggtgtccagc agggagatca ggttattctt cagcatggtc tgaactttgg
     1621 aatactgttg gtactgccgg tagcagattt tcagcatgag tcgggtatcc tcctccggga
     1681 tatatctcgg caatgtgagc cagtggtcaa gaccgtagtt ggccagcttt acggcatcct
     1741 ttttgtcggt tttggcccgt cttaaactgt tgtttccgta gtcatgcacc agcattgcat
     1801 tgactaccga gacataaagg cccgcgtcgt ggagcagcca ggccaccgga gcatggtaat
     1861 tacccgtgga ttccatcacc acacgggtct cgccgtccag gcttttgagc agtcctgcca
     1921 gctcgctcag ttcattggcg gtgtgacgta cttcaaaggg tgaaaccact acctctccga
     1981 agggccgcat gactgcaatc atgctcttac ccttggaaac atcgatgcca acgcagttca
     2041 ttcacattcc tccaatacaa agaatctgca accggaatcc atcttttctc gctgccgatt
     2101 caatctattg tgtgacacga actctccgac atccggtggt tcaacctgct taaatcgaac
     2161 gctgcaacga gaggatggct aacagtcttt cacacggaca tacaagccca aggagtgatt
     2221 ggtcagccag ttgctcctct cattgtagct taggcacgag atggagggaa agccggactg
     2281 gctgtccggc tttcctgtcc aaatttattg taataggagc gaacaacgct atggcagttt
     2341 tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa tcaccacctg cggaataaga
     2401 acctgacctt aaaggccaag ggcctgctgt cgcagatgtt gtccttgccg gaggattggg
     2461 actataccct aaaaggactg tcctatatca accgggaaag catcgacgcg atcaggacag
     2521 cggtatggga gcttgaaaaa gctggatata tccggcgtga gcagggccgt gacgcaaaag
     2581 gcaaaatggc cgatatggtc tataccatct acgaacagcc ggtattggaa aacccggtat
     2641 tggaaaatcc aacatcggat aacccggtgt tggaaaatcc gacatcggat aatccggcgt
     2701 cggaaaatcc aacgcaatta aataaagaag tacaaagaac tgacttatca aaaaaagaaa
     2761 aaataattac agatggacaa agtacccatt ccattcctat cccatccccg acccctgccc
     2821 ctatggaggg cgaggcagcg gctacgccgc cggaacggaa aaggacagga aaggatgagg
     2881 ctgtacggat ttatcgggaa atcattttgg agaacatcga gtatgcctat ctcattcagg
     2941 acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga cctgatgctg gaaaccgtct
     3001 gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta ccctgccgag ctggtgaaat
     3061 ccaagtacat gaagctgacc ggcgaacata tccgctttgt ccttgactgt ctgcgggaga
     3121 acacaacgaa agtccgcaat atcaaacaat acctgcgggc tatgctgttc aacgccccca
     3181 gcaccatttc caactactac acggcccttg tcgcgcacga catggcacag cccgattggg
     3241 gaaagcctaa atccggcctg ccggactatt cctgcggcga gggcgaaagc ctgtaacgag
     3301 aaaaggagga cgaccctatg aagcagggcg cattgatttt tgacgagcga acagaccgtt
     3361 acgacatccg ctttgatctg gccgactatt acggcggcct gcattgcggg gagtgtttcg
     3421 acgtgatgat cggcggcaaa tggaggccca cccgcattga aatggccgaa aactggtatc
     3481 ttgtcggcat ccgggccgac gacctttccg gcctgcgggt gcggatttga cctatatcta
     3541 cctgcggcgt ctggtggttc cgccttagac gccgcttttg actttctgac aaggagggat
     3601 gcgattgcag gaagaagtta ctcaaaagac cattgccctg tccatgaaaa cgggcaagct
     3661 cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg caacaccggg ccaagggcaa
     3721 aaacacgctg caccacggcc agcagagcct aaagcagctt aaaaagcatg gggctgatct
     3781 ctccaacatt gagatcaccg aggccaacat tggagcgttc aagccatgcg ccaagaaata
     3841 cggcgtggat tttaccttac gaaaagataa gaccacccag ccgccccact acgttgtgat
     3901 tttcaaggcc aaggatgcag ataaccttga tctggcgttt cgggagttta ccgccaagac
     3961 gctttccaag gagcagcggc cctctatccg caaggttttg tccgctgtga agcaaaaggc
     4021 ggcggcccag accaagcagc gggccaagga gaaaatcaag gaaagggggc tggaactgtg
     4081 aagcctgacg taaagaaatt gctgatcctg aacctcccgt atctgatttt tgtgtacctg
     4141 tttgcgaaat gcggggaggc gtaccggctg gccgctggtg ctgacctgtc ggggaagctc
     4201 ctgcattttg cggatggttt tgcggcggct tttgccaacc cgctgccaag cctgcacctg
     4261 tttgacctgt gtatcggtat cgcggtgccg tgatcgtgcg gctggtggtg tactgcaaga
     4321 gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc tgggggacgg
     4381 ccaaagacat agcgccttac atcgacccca agtttgaaaa taatatcctg ctcacccaaa
     4441 cggaacggct taccatgacc ggcaggccca aagaccccaa gacagcgcgg aataaaaacg
     4501 tgctggtgat cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa cccagtgcgc
     4561 ccgtaagggc ggtataaatc ctaccttgag caagtagtag gtaaaagtat caagcggcat
     4621 tgtgccgcaa cctatcattc cccgtcttac cccaaaaggg aaaccggagg gcgaccatag
     4681 catgacggag gacacggggc gctgaagcct cagaagcgcc ggcgtgacgg aatgaaaatc
     4741 cacgtatgag gatggaagct agactgcttg aatgacagcc tgaaatcggg accaagagcg
     4801 tacccctgac gtagagaggt tgtactcgtc agtgttcctg acagtcgcat tgtttggcta
     4861 tgcggctgct gatactcgga gcaggttcag ccacgggaaa gtggaaaaag gcgaacgtca
     4921 cataccgaca acgtggaacg cttgtttata ccgtactaaa cggggattgc ctaaagtgaa
     4981 atgccgaaag gctatgaaaa cgctgaaatg cggggttctg aatattcacc atggcaacag
     5041 agttcccata gtagtctgcg gacgggaaag ccgtctacat ggcaaagggg aacagtgaat
     5101 cattttcaaa acagaaagga tggtgtgtga gacactatga gaaatccgat tcatgtcttg
     5161 aaaagccttg aagaaaaggc aagtgtaagt aactacaaat atgaaagatt atatcgcaac
     5221 ctatacaatc cagagttcta tctccttgca tatgcgaaca ttgcgaaatc gcaggggagc
     5281 atgacgcagg gggtagacgg gcagacgctg gacaacatga gcctgccgag aattaaccgg
     5341 attattgaat ccatacgcaa caggacatat cagccaaagc ctgcaaaaag aaagtacatt
     5401 cctaaaaaga acgggaaact tcgcccgttg ggaatcactt ctactgatga taagttagtg
     5461 caggaagtgg tcagaatgat tcttgaagca atctatgagc cgacattcag caacaattca
     5521 cacggtttca gaccaaaaag aagctgtcac acggcactta cgcaagtgaa gaaaaacttc
     5581 acaggtgtta catggattgt cgagggagac attaaggcgt gctttgataa cttcgaccat
     5641 catgtgttgg ttgaattact gcggaaaagg atttcagacg aggcttttat cggtctaatc
     5701 tggaagttcc tgaaagccgg atatatggaa caatggcagt acaactgcac ctactccggt
     5761 gttccgcagg gcagcggtat cagtccgata tgtgcaaaca tctacctcag cgaactggac
     5821 aactatatgc aggaatacaa agaaaagtat gattgcgagc cagaacgcag aaggacgacc
     5881 agagaatacg agcgggcgtc ccggagatac agaaaggcac gtaaagcgtt aatgggcgca
     5941 gaaaaatcaa ctcctgaact ggtaaaagaa tttaaggatt ccagaaggaa gaagatgaat
     6001 cagcactatt acaatccgtt tgaggaaggc ttcaagaaaa tccagtacaa ccgttacgcc
     6061 gatgattttg taatcggcgt tatcggctcc aaaaaggacg cagaaaaaat caaggaagat
     6121 gtaaaaatct ttctccaaga aaaactgcat ttggaaatgt ccgaggaaaa gacgaaagtc
     6181 acccattcca gtaagccggt acgctatctg gggtacgatt tcaaagtaat ccattccaag
     6241 aacatgaagc gttgtaaaaa cggcgacatg aaacgggtgt ggtatggaaa agtattcctg
     6301 tatatgccga aagaaaaatg gattaaaaaa gcgatggaac ggggagcgat acaggtgaaa
     6361 cgcaacaatg acacaggcaa agaaatgtgg cggcctatgc caaggaaaga cctgatgaac
     6421 cgcagcgacg cagagattgt gtctactttc aattccgaaa ttcgagggtt atataacttc
     6481 tatcggatag cagaaaacgt aggcgcattg cacaagtatt actacatggt gcggtacagc
     6541 atgttaaaaa ctctggcagg aaagcacaga acgaatgtca gtgttattaa gaagcggcac
     6601 atggtaaacg gagtactgag aatcccatac gatacaacta agggacgtaa atactgcgaa
     6661 ttttaccatg atggattcag aaagcacagc gacggctatg acaatgtggc agacgttatg
     6721 ccgagttata ggaaatatga cagcaggcac acgatagtaa accgcataaa agccggagta
     6781 tgcgagattt gcggggaaca tgcagactat ttatgtatgc accacgtaag aacgctgaaa
     6841 tcgctgaaag gcagggatat atttgagcag aaaatgctga aaatgagaag aaaatctctg
     6901 gctctctgcc ctgactgctt tgaactctta cacgaaacca aagaatcaag gtgattcatg
     6961 gaaagccgga tacgctgaga ggtgtacgtc cggtttggga ggcggctccc tgaaacctac
     7021 tgatgaaagt cagcaaggcg cagggtgcct acctcatacc tgatgcaatg ctttccgacc
     7081 acggactatc cgacttcgtt tgtggtgaca gatccgaaag gcactttggt tctggaaacc
     7141 ggcaggatgt tccagcaggc gggctaccgc attaaaatcc tgaataccat taacttttcc
     7201 aagtccatga agtacaaccc ctttgtctac atccattcgg aaaaggacgt gctaaagctg
     7261 gtaaataccc tgatttccaa caccaagggc gagggggaaa agtcagcgga ggatttttgg
     7321 gtgaagtcgg aacggttgtt ttattcggcc ctgatcggct atatctggta cgaggcccca
     7381 gcggaggaaa tgaacttctc aacgctgctg gaaatgataa acgccagtga agcccgcgag
     7441 gacgacccgg agtttcaaag ccccgtcgat ctcatgtttg agcgtttgga gcagaaagac
     7501 cccgaccatt ttgcggtgcg ccagtacaag aaattcctgc tgtccgcagg caagacccgt
     7561 tcttccatcc tgatttcatg cggtgccaga cttgcgccct ttgacatccg ggaggtacgc
     7621 gagctgatgg aggacgacga aatggagctg gacaccatcg gggacgaaaa gacggtgctg
     7681 tatctcatta tgagcgatac ggacacgacc tttaatttca ttctggctat gctccaaagc
     7741 cagctaatca acctgctttg tgaccgggct gatgatgtgt acggcgggcg gctgcctgtc
     7801 catgtacgcc tgattttgga tgaatttgcc aacattggcc aaatacccaa tttcgataaa
     7861 ctcattgcca ccattcgaag ccgtgaaata tcggcctcta ttattttgca gagccagtcg
     7921 cagctaaaag cgatttacaa ggacgccgcc gagatcattt cggataactg cgatagtgtg
     7981 cttttcctta gtgggcgcgg caaaaatgcc aaggagattt ccgatgcgct ggggaaagaa
     8041 acgattgaca gtttcaacac cagcgagaac cggggaagcc aaacgtccca cggattgaac
     8101 tatcaaaaat taggaaagga gttgatgtcc caagacgaaa ttgccgtgat ggacggcggc
     8161 aagtgtatct tgcagctacg gggtgtgcgc ccgtttttat cggagaaatt cgatattacc
     8221 aagcatcccc ggtacaaata ccttgccgac gccgacaaga agaatacctt tgacgtggac
     8281 agatttttga ctatcacgcg ccggaaacgg cagcaagtgg taacgcagga tgaagtattc
     8341 gacctgtatg aaatcgacct gtcggacgag gacgccgccg ctgaataaac agcggctttt
     8401 ttcattccaa caacaaaatc aaaaaatcat tatgagccgg aaaccggcag aaagaggtaa
     8461 atttatggca ttttttaatt ccgcagttac tactttgcag actatcgtga tcggactggg
     8521 cggcgctctg tgcgtatggg gcggcatcaa tctgttggag ggatacgggc aggacaatcc
     8581 gggggcaaaa tcgcagggtg tcaagcactt aatgtcttct atgtgaaaag attttaaaga
     8641 gaaaatgcac atcctacgca gataacatga caatttcatg caaagcgaaa ggagacacca
     8701 tgaaaatcga atacacaaaa gtgggagact attatttacc gaatctttac tacccggagg
     8761 aaaccagacc gattggctta tggggaatgc ttcggaagga atatctgaaa gaacataaat
     8821 ctggaactta tacatatctt cttctgaccg ccaggctgga cagttatttg gcagatctga
     8881 atgaacaggc acaggaacgc tttgaattga tcgaaactca gatgcgaagt gcggagggag
     8941 tttccgagga actgaagaga cagaatccta tggaatggct atgccactgt aacaatatcc
     9001 gcaaccgggc tgctgagatc atcaagcagg aactgattta tgtataaggg ggattctaga
     9061 tatgagaaga gaactgattg aaagttatta tgaagaatac cagcgggagc tggaaagatt
     9121 tcttgaatct gacccggaga tgttcactct ggaagcagaa attcatcatt acatagaaat
     9181 actggaaccg gtcctcagag atggcggaca ggagatgtgg cagacactgg acaggattat
     9241 ttcagcgaaa aatgcaatgg agggaattgt ggcaaaagaa atgtatatcc ggggatttct
     9301 ggattatgaa agactggtgt gtgaaaaaga cagtaagatc tgaataatca gaacatagac
     9361 tatatataac gaggctttta ttgtatagac agtaagagcc tcatttaata tagttttcat
     9421 aataaaaaac aggtggtagt acgtatttgc tttgtgttat ataagaattg gtctggctga
     9481 acaatggtga aaatttacta tgattttact taaatctgat tgaaggatga aaaagtgatg
     9541 atatcatata gtgggttgat agggttacag aaaatgtatt cacagaggca gagaagaaat
     9601 aatgaaagta aaaaatgcag atgaatttta taaaatatta gaaagaaagg ctatatatcc
     9661 ggtatttcag ccgattgtaa atttacagac tggagatgtg gcaggatatg aagctcttag
     9721 tcgtatcgac aggcatgata cgactcttat gatttcagat ttgtttgtga ttgctgaaca
     9781 ggtaggctgt gtttggaaat tggaaaaatt gtgcagaaat aaagcattga aggcagcggc
     9841 gaataaacca gaacatgcaa aattatttct taatgtagat ggtaatatca ttcaggataa
     9901 atcgttcata cagggattta caaaccgtaa ggctgcgaaa gccggagtac cgtcctgtga
     9961 cattgtattt gaaataacgg aacggtcgga tatagaaaac tatcagattc tgcaacagat
    10021 tatgaagcat tatgcagatc agggatatga aatagctttg gatgatgttg gagctggata
    10081 ttctggtctg aaccgtgtag taaatacatc gccaaactac ttaaaagttg atatagaact
    10141 ggtcagagat attcaaaaac ataagaaaaa ggaaattatg atggaatttc tgttgcatta
    10201 ctgtaatgag acaggagcaa ttctgattgc agaagggata gaaacagaag cagagttgga
    10261 gtgtctgtgt agattaggtg ttcattacgg acaaggatat tttcttggaa gaccggacag
    10321 aacgttcaaa aatgtaagga atgaggcgat gttggtatta cgtagactga ataataaatc
    10381 gtaatgaaaa ctatatgtca gcaggtgaaa aatgaataag tcaattataa cattataaag
    10441 atttccattg atttttacac ctattttaca caagcactca catagatttg atttctgtct
    10501 tctataattg tataggaaaa tgtagatctt atataataac aggagaggaa agagaaatga
    10561 acgagaatgt gaaagtggtt tttggtctga tcggtggcct tgccttgttc ctttatggaa
    10621 tgaacagcat gagtgatgca cttcagaaag ccgcaggaga acgaatgaaa aaaatccttg
    10681 gatttcttac aagaaatcca attatgggag cacttgccgg agcacttgta acagctgtat
    10741 tgcagagcag ttcagcgacg acagttatgg ttatcggttt tgtcagtgcg ggacttatga
    10801 gtcttccgca ggcaatttct gttatctttg gtgccaacat aggaaccact atgacggcac
    10861 agctgatggc atttaaaatc agcaattata tttatccaat tatctttgtt ggatttattc
    10921 tgaactttgt tagcaaaaaa gaaaaggtta aaaatatcgg tatggtaatt ttttcattcg
    10981 gacttttatt tgaaggaatt gaaatcatgg gtgaagtcat gaaacctttg gcgggaagtc
    11041 cggtctttgt tgatctgatg ggaaaagttt catccattcc ggttcttggt gttgtactgg
    11101 gagcggtcat gacattggtt gtgcagagta gttcagctac gattgcagtt ctgcagaatt
    11161 ttgcatcaca ggcaggaccg gacggagtaa gcagtgtaat agggcttacc ggtgcgattc
    11221 cgattcttct tggagataat ataggtacaa cgatcacagc acttcttgca tcaattggac
    11281 aatctaaaaa tgcaaaacgt acagcaattg cgcatagtat ttttaatatc agtggaagct
    11341 gcgtttttat tttcctggtt ccgtggtttg caaaatttgt ccagtttatt tcaccaaagg
    11401 gaaatgagat tgatgtgatt tcaagacaga ttgcaaatgc acatacaacg tttaatatcg
    11461 tctgtacact ggtctggctg ccactgattc cgctgatggt aaaaattgtt acaacaatca
    11521 tccgtggaaa tgacaaaaca gagaaagcag cttttgaacc caaatatctg gatatgaaag
    11581 tgattgaaca gccggcggct gctatggttc tggtatctaa ggaactgaac cgacttggag
    11641 aactggcaga atctctgtta agtgatttaa aaacggcgat tgtagcagat ggagactcaa
    11701 aaactcatgg ttcgtttata gaaaatctgg agattgtaca tcagttacag gacagtgtga
    11761 cagaatatat taccagatta ttcgcaagcg gaaaccttac agaacaacag tctgagcaga
    11821 cggcagggtt actgtatgta aacaatagta ttcagaggat tgcagacaga tgtgaagata
    11881 ttgaccagat atgtgaaaaa gtaaataaca atggaaaaag actgaccagt gaagcctcct
    11941 cagaaatgga agactgcatt gatattatcc agcagctttt aaagaaagca atggaggcaa
    12001 tccgtaaggg tgatgctgta gtggctgaaa cagtttttaa aaataagaaa aaaatgcata
    12061 aagcagagaa aaaatacagt aaggcacatt taaaccgtgt aaaaaatgag gtttgtgatg
    12121 cgtctatgac aagatatttt tctgggatca tgtataattt tgaccgtatg gcagataatt
    12181 gtgtcagcat tgcagaagaa gcctgtgata atgtggcttt tataaacctt gatgaagaga
    12241 cgggaaaatc aatgatggaa agaggtgcag tataaaatgg taaagaaaaa aaacatccga
    12301 aaatctgcac ttctgctgat aatgataatg ctggcaacgg gaataacagc atgtggaacc
    12361 gaggaagagc cattgccgga tctgtctgct atgggagctg tcacagctgt atcacgagaa
    12421 gaagggtcag gaaccagagc agaatttgag aatcttttga aactgcctga gagtgataca
    12481 ggaatagttg ttgattctac agagaaagtt ctgaaaaagg tggaagaaga taaaaacgca
    12541 gttggttatg tcgcatatag ttccgcaaca gatacgaatg gaaaaatact tcaaatcaac
    12601 ggagttcttc cttctgagaa aacaatagac aataacagtt atcctctttg cagggattat
    12661 tatctggctt ataatggaga actgacagat gtggaacagg atttcctgac ttatgtaaag
    12721 agtaaaggac aggatattgt gaaacaatat tgtattcagg ctgacagtac aacaacattc
    12781 ctttctgaca agtcagaagg aaagattctg attgaaggat ccacttcaat ggaaccaatg
    12841 gtaaaagcac tggcagatga ctatcagaaa caaaatccaa atgctgaaat agaagtaaaa
    12901 gcaacagatt cttcaagagg gattacggcg gtgataagtg gagagtgtga tttcgcaatg
    12961 tcatccagag aattaaagga ttatgaagca gagcttctgg aaacaaaagt cattggaaaa
    13021 gatgctatag ctattgtgat aaatgaagaa aatccactgg aaaatcttac aataaaacaa
    13081 cttacaggtc tgtacaatgg aacatataaa aactgggatg atctttcctg agaaaaacga
    13141 atataatatc ttacaagcac tgactataac ggtcggtgct tcttttttgg attttttcaa
    13201 aattatctga cattcttcaa attcccacgt gtgattagtg gaaaaataat tttttctgca
    13261 aaagtaccgt ccaaatgccc tctgaaactt gctattagtg agggaatatt tcatttatgc
    13321 gaaaaaaact tgcaaaaggg ggttggtaaa taccgcttcc caggctttat aggtagagga
    13381 acaattattt taaaaatttt accccaaaga tgtccgcttt tgcctgtctc agtacagagt
    13441 atgtgaaggg actaacaaaa gtaaaagaaa aaatgataaa aatgcaaaaa aatatcacga
    13501 atcttttgca tcttgcgaag tcatttgact ctagtaatca gaggacgttt tggaatgttc
    13561 cttcgatgaa ccttgacaac cgataagtgc accctagaag cctaacagga gttatgccat
    13621 gacctttctt tttctgagag cgagcgaaat tataatgaca ctcccgatga tgggcggctg
    13681 accgaagcct tatggtcagt gacctgctga tgcaggcaga ggcagtctga gacggctgca
    13741 ggtgacaggg aaacgcacgc cagaccgccg gacgaatgaa actgatgaca atgtttacag
    13801 ggcaaaatgc ccggaaagga gcagtttatg acagaactga aaatgaaaga aatgcagttg
    13861 attaactggt ttggatgccc gaatctgatt tatacaaggg agagaatcca tctggttgcc
    13921 atgcttacaa cagatgaaga aatgaaaaag aatctttaca gggtatgcag attccttgca
    13981 agagaagaaa tcgcatatcg gtatccgatc atgtatgaaa tgatccggaa aatattttca
    14041 ccggatgata ccaatccacc ggaagcatgt gcaatgttta tgccggacga tgaaacagat
    14101 gatgaagaat atgcaatggc agcgtagctg cggaaaggac agtaacgatt atgaataaag
    14161 tggttttaat gggaagactg acaaagaatc cggagatcaa atatgccgga aaagataatg
    14221 atatggcagt agcaagatat acactggcgg taaacagaag atataaacgt gacggtgaac
    14281 aggaagcgga ttttatttcc tgtgtcacat tcggaaagag tgcagagttt gcacagaaat
    14341 atcttcacaa gggaatgagg attgtgattg gcggtcgtat cagcactggc aattataagg
    14401 acaaggacgg gaaaacaatt tatactacag atgtgatcgt ggaagaacat gaatttgccc
    14461 agaacaagga taatggtgtt ggagctgatt catcggaaac accgaaaaca gataaggacg
    14521 gatttatgga agcaccggaa ggtgaggttc cattcgacta aaccgaagta aatggttgga
    14581 gaggttgccc tttgtcgggg catcctcttt tccattttac agaagacatc aaaaattaca
    14641 ggtattcaac ctaacgaaat cgtgatataa tcaaaatatg ttcagatgaa aaatatggag
    14701 gttgcttatg ggcgtaagtt ataacagatt atggaaaatg ctcatagata aaaatatgaa
    14761 gcggatagag atgcagtacc tcaccgggat cagtggaaat attcttgcca ggatgggaaa
    14821 aaaccagtat gtatcaatgg agacgattga gaagatctgc aagaagctgg actgcaccgt
    14881 tgatgagatg ctggaattta cagatgatga gtaacaagag cagacatgtc aaattattaa
    14941 aactggaaga atgcttcaga agtcgaaaaa agaaattgaa aaaagataac acgccggata
    15001 cggcataaat ctcttagcaa cgctaagagc gcacttctat acaggcaatt ccatctgcct
    15061 gtatcgtgcg acctgcggtg ctgttcccga tttcaagaaa tctcaaaccg ggaaatacag
    15121 cgaaatcaga gatttcgtac aaggggaaga tccccttgcg gcacagatgc ctatccctga
    15181 taaagtttgt gactgaatat ttcagatata gacatataag gggaaagaaa gtgtgaaatc
    15241 aaaatttgtg gagagatttt atgattagaa aagcaatact aaaagatgtt aattcgattg
    15301 aagatactta caatgagcat ttccagtatg aactgaatca tacagccttt actgtattta
    15361 agaaaggggt ttatccaacg aaagatgatg cagaacgggc tatttatgct ggtgctttgt
    15421 ttgtatatga agaaaacgga acaattgttg gaagtatcat tattgataaa gttcaaccaa
    15481 tagaatatgc aactattcct tggaaagaaa aattatctga agatgaggta atggtaattc
    15541 accttttaat ggtacgtccg agtatgtctg gtaaaggaat tgcatcatca ctaataaaat
    15601 tcgctacaga attagcacaa aaaaatagtt gcagggcact acgtttggat acaggaagtc
    15661 aaaatattcc tgcgctatct ttgtatcaaa aaaatggatt tgaaataatt gctagtgctt
    15721 ctaaaaaagt tggagatgta attgcacaca aaaaccatct ttttctcgaa aagatattgt
    15781 agttcgtaaa ttccagtttg aaaaattgag aaaatcggag tttgaggagc atcaccgtta
    15841 tgattagaga attacggaaa gtggatataa ataaagttgc agaaatatgg ttagatacaa
    15901 atataaagac acattatttt atctctgctc aatattggaa aagtaatttt gagttagtaa
    15961 aagaacttct attacaggca actgtctatg tttacgagga taaacaggaa atacagggtt
    16021 ttatagggtt aagtaacgaa tacattgaag gtatttttgt ttctgccgaa atgcaatctc
    16081 aaggtatagg taaaattttg ctaaactatg taaagggaaa aagaaacaaa ttaatcttaa
    16141 atgtatatca aaaaaataca cgggcaatat ccttttatca aagagaagga tttgagattc
    16201 agtatagcgg tttggatgaa gctactggag aaaaagacta tgtaatggca tggcagcaga
    16261 aatagaaatt caagttttag tgtttggaaa attatgactt agtgtgcaag ttgggagaaa
    16321 atatggtaac ggaaaaagag aaaaaagcat gtcgatttat tgccgaatgg ctataagatt
    16381 gtgagacagt ctctggtaga tgatctggag ggcgaaaaaa aggtatgttg ggagacagaa
    16441 attttgcagg atgatgatag tggatttact tatgaaatca gcaggtgtct gtatttcgat
    16501 acctgtaagg aacatggcta tgcagagttc tgcaaagtat tttgcaacca tgactggtat
    16561 gcttatggag tattaaagca tcattcgaga tttatcagaa aatcgacgat tgccgaagat
    16621 ggaactgtat gtcatgatac aatcgagaaa gtaacagaga aacatttttt ataaaaccga
    16681 aaaattgatt atgggaggaa attgaaaatg gcgaaagaag agagatttga aaaaatatat
    16741 tctcagggag cattaactac tactgagata tgggtggata aggaaactgg tgtgaattat
    16801 ttatatcatg catgtgggta ttcaggtgga ctgactccat tgttggacag agatgggaaa
    16861 ccagttattt ctcaggctat aaatagatga ccttagaaac agcaactgtt atggtaagta
    16921 acagcatcgt cacaatatgt atatggtcga ttggtgtgtg cggtaatata ctatattcag
    16981 aaaaacaatt caggagattt ttatgagaag atcaggttgg acaaaatggt tgatcgtgat
    17041 tccggcaatg ctaggcattg tgttggtgac ctatgtgcgg tatacaacgg atatctgggg
    17101 atttggtgca tttatctgcc agctgattgc aattttggaa ttggcttatg gtctgcgaat
    17161 tgcgatgctg gcacagaata gaaagaagtc ctatcggtta acaccggaag aacgacatga
    17221 gtatgcacgg tatttatatg agaagcaata tcaccgttac cctgctgtag cgaatcagat
    17281 gcttttggtt atggcacgga tgagtgtact ccttaacaat tatgaacggg ctgcacagga
    17341 gctggcagat attcgtatag ataaattcaa tccagcacag ttgaagttgt attattacct
    17401 gaaagtggtt acagcaatgg cagcaggaga tgcgacaggg attcaagaga gccagatgtg
    17461 ttacgcaggg attccggata ctaaatgcac atatccgtca aaagcagagc ttgctatgtg
    17521 gatagagcag agagcggcag cgcagatggc agaggcactg aagaaggcag tgccggataa
    17581 aaaagagcat ccgatacgga tcagtatcat tactttggtt cttgcgtata gtaccttctt
    17641 ctatggattg tggtatggta taaaacgtga tatgggatat gaagtgagat atcgtttcgc
    17701 agaaatatcg attactgtta ttacaatcag tctggcggtt cttttgatct gggggatcgt
    17761 gagtttatac agacacaatt gccaggcatt atccatacaa ttcggtaaaa acaaagtggc
    17821 attgataatt gtctatggag ctgctgcgat atttgcactt ttgattattg gaatgagtgg
    17881 tctgagtgta gtgttcggaa tggatggaac agaaacggtg actggttgtg acagcaaata
    17941 tacgtatata actgtgcagg aagattatgg tggtgaacgg agatacagaa ccaataatcc
    18001 atttgttatg cagagtatgg atggtctgtg gtggtcttcg gatacaatag atgaagaaga
    18061 taatcaagaa gaggatacta cagaattacc ggatttggat acagagaatt caacagattc
    18121 agaggatatt cagagtggaa ataccataga agattacgag acagaagggc agcatgatgg
    18181 tctgacaatt caaaatgcaa tgctggcagt ttataattac cttcaggagc agaatacact
    18241 tcaaaatatg gcgttcagtt acacggcgaa tgcaaaggga gaagtctatg ctgtcgtcag
    18301 cgaaacgcag gaaacaaaga acggtaatac ggtaaatgtg agatactgtc tgtacgataa
    18361 tggttccaag acagatgcag atggtaattc ctgcgaagaa ctggtgttag aaaaagtata
    18421 tccggatggc aattacgaga ccgaattggt agatttttat ctggtaaatg cagatacgat
    18481 gcaggtgaca gacgaacaga agaatacgtg gtaaaataaa ttctggggaa gtaggtgcat
    18541 tgtatgacaa tgagagaaaa aataaaacta ttatcgtaaa tgtttcaagc ggactgggtt
    18601 cttttggaag tatgcaaaag gactccaaga cataagaggt gaactgcgct gatccaggtg
    18661 ccacggcaac agatttgaat gtcgacggag gaaagcaaac agtacaggcg caatcgtaaa
    18721 acttgcaatg attgataaaa ccggactaac agtaactttt attagtcgat ttggaaatat
    18781 gccttggtaa tcaacaattc cggttttact gactaagaaa atgaggagga ttcataatga
    18841 gtgagaaaac agagattact tttatgcaaa caagactaat ccggcttgct tcagaagagt
    18901 ggcatttacc tgttgaacag attatccact tgtttaaaga agccgatgtt cttggatata
    18961 tagaaaaatg ctatgggatt tttcattgtg aaggcgatga agctgtgctt gaagatatta
    19021 ctgaattttt gcaaagaaag gggatagaga ttagtgcttg agttaaaaga tggatttgtt
    19081 ctctatcatg gaagttattg tgaagtaaaa gagccagatc ttgccaaatg tgcaaagaga
    19141 aaagattttg gacagggatt ttatttaacc acatcaaagg aacaggcaga aagttttctg
    19201 agaacctcaa tagccaaagc catcgcaacc ggaacaattg aagaggggca gaagtttgga
    19261 tatatttcaa cttttgaatt caaactttca gaaaatcttg aaacgcatat ttttgaaaat
    19321 gcagatgtgg actggttaca ttgtatcgcc gctcaccgaa agaaaaaaat gtttattgag
    19381 gttgaacgtg aaatggcaaa atatgatatc attgcaggaa agattgcgga tgatgcaaca
    19441 aacgctactc ttaccgctta tcttgcgggt gcttttggga ctgcaggtga taaagtagca
    19501 gacgatttct gtatcagaca gttgctgccg aataaattga aagatcaata ctgctttaaa
    19561 actgaggcag ctattgagtg tctgaaattt gtgaaaggtg agaagatatg gctgaagtaa
    19621 caattccaaa ggaaaaaatg aattatacaa tcgaccttct gatcactatg gtgacagatg
    19681 aaatcgcaga agagacaggc aaggacagaa acgaggtatt gacagatttt ttgtgttcaa
    19741 agacaggaaa agcactgtat gatgagaaga caaagctgtg gtgcactggt ccggcatata
    19801 tagctgaact gtatatggaa gaattaaaga aatcctgata tgggatgtga gtttaaattt
    19861 atacgacaat ggtgacaaag actttcattc caaggagcaa caattaaata tttcaaaaac
    19921 gctgaggtca gcatttccta atcacaggac aacgacctca gttttttata cccaggaatc
    19981 ggcattcatg aataagccgg ttctttttgt ttacagaaaa aagaaggagt acaagaaaat
    20041 gagcagagca aaaaatccaa agtatgaatc catgtcattg gaggaactga aggccaacat
    20101 ggaaaagagc agacaggaac tggaggcagc aattcaccac aaaaacattc tggagcagcg
    20161 gaagaagctg gtagaacgca gggaacgttc ccaccggctg attgtcaaag gggcagagtt
    20221 tgagaaagta tttccgctgt ccagagatct ggaacaggaa gacgtccagg acatgatgaa
    20281 tcaactgcag aacagcagtt acaacaagag tattgtgcgg caggctcata tagccgcctt
    20341 acacaaagat caacagcaga ttgcagaagc agtaaaaaag acagagaagg gagatgacag
    20401 ttaatggcac tgtatcattt ttcagtaagg aatgtatcca gagggaaagg gcagatggtg
    20461 gtcgcttccg cagcctatat ttccgggcag aggatttttg acagttatta caacaagata
    20521 cacgattata cgagcaagtc aggtgtgata tttacagaaa tcctgacacc ggagtatgtg
    20581 ccagagcgtc tgactgacag ggaaacactc tggaacgagg tggagcatgt ggaacggaac
    20641 aacaaggcac agcttgccta ttcgtttgac attgcactgc agaatgaatt gacacttgag
    20701 gaaaatatca gactggcaaa agagttctgc caggagcagt ttgtggcacg tggcatgatc
    20761 gtggatctgg cagtccatga gggaaaagcg gaaaatgaag aagaaccgga taatccccat
    20821 ttccatgtgc ttgtcccaat ccgtcctttt acagaggaag gaatctgggg caataagcag
    20881 agaagggaat atattcttga tgaggatgga aaccgggtga aggatgccaa aggaaaagat
    20941 atgttcaatg ctgtttctac taccggatgg aatgatccgg agcttttaaa agaatggcgg
    21001 cgggcatgga cagagaaagt aaatgggaaa ttcagggagt gccacatggc atccagaatc
    21061 gaccaccgtt cctacaaaga acaggggatt gacctgatcc cgaccatcca tgagggatat
    21121 gaagtccggg caatggagaa aaagggaatc aaaacggtga tcggagaatt gaaccgggca
    21181 atccggcagt ttaaccagat gtttatttct ctgaaagaat ccattcagtg gatgaagaca
    21241 gcttatgagg aaatgaaagc agagctggat cgcaggcaga acccgacact gctggaaagc
    21301 ctgcaggatt attatgataa aaagacacag ggcagaccac cacttcccaa tttttatgcg
    21361 gagatgaaac agagaggtaa gaatctctcc aatttacagg agtttgcaaa atccatcagt
    21421 tatcttcaga cacatcagat agaaacgatg gatgacttgc aggaacggat tgaggaactg
    21481 aacggtgttg tttctgtcag taaaaaagaa atttctgaaa agcggaaaca gttaaaggaa
    21541 cttgagaatc tggaaaagat ggcagaggta ataaaaacaa atcagccatt gattgatgag
    21601 tataaccatt tctattttca gaaaagacgg gagagatatt atcagcagca taaaaaagaa
    21661 atcaattatt accgcaaatg tgaacgggaa ctgaaacaac atctggataa aaatggaaaa
    21721 gtgccaactg ccagatggaa acgggagaaa gaagaacttc aggcggggat cgaagagctg
    21781 aaagcagata atcagccgta ccaggaggaa ctaacttttg tgaaaaaggt acagagctgt
    21841 gcggatattg ccagacgtga ccgggaaatg gcagaagcag atacttccgg gcgttcagaa
    21901 gaaaaaaggg agaagcaggt gaagttcccg acattttaca cagttcaggc gaaagagatt
    21961 cttgaagaaa acgggaaagc agagcaacag ccgttgaacc agtcggaaca gaatccagaa
    22021 aagaagacct ctcttctgag aaaactggat gaaaaaaaga aagagtgtgc agagcgtgat
    22081 gcaaaacaac aggcagtgaa gaaaaagcga aactatgaca tgagtttata acagtctgtc
    22141 agcaggacag acgggaaagg atgagcaaaa tgcagtatac agaagaacaa attataagag
    22201 caaatcagac agatcttgtt tcttttctga gcgcacaggg agaacaacta gggaaaagcg
    22261 gaaaggaata ccgttggaaa aagcatgaca gtgtaacggt atccggaaac cggtggtaca
    22321 ggcacagtca ggggaggggt ggctatccgg tggattttgt gatggaattt tacaatgcca
    22381 catttccgga agctgtcaag atgctaaccg gggaggaagg cgaaggcagg aacagtacct
    22441 gccccgcccc gtcccctgac ttccgattac cagagaaaga ggaaaataat gatagaatca
    22501 tcagatatct gacagaaaac agagggattg aaaagaatat ggtggaggaa tggatcggca
    22561 gtggggatat ttatgaagaa aagaagcacc ataatgtggt gtttgttggc agagatgcag
    22621 acggtattcc aagatatgcc cattgcaggg gaactggtga aacaaaatac cggggagatg
    22681 tggctgaatc tgataaatct tacggattct gtcacagagg gacggataat cagttgtttg
    22741 tgtttgaagc agccattgat ctgctttctt ttatccaatt atttcccaaa gactggaaaa
    22801 agcggagcta cctgagtctg ggtggcattt ccagtgcggc actgatggct tttctttctg
    22861 aacgccctca gattacttcg gttttcctgt gcctggataa tgaccatgca gggaatgaag
    22921 ccagtgagaa attggcaata gaaatcccgg acggatacag tgtcatccgt ctgaaaccgt
    22981 cccgaaagga ctggaatgaa atcctctgtg ataaaaatgc agacaggaag aaatcaatca
    23041 ttgaaacagt tacaatgaaa gtaccggaga aggaagaact ggtgccgatg ttgtgttatg
    23101 aagacattga acagaccagt gtagaatggc tgtggtttcc ctatctgcca tttggaaaac
    23161 tgacgattat tcagggcaat ccaggagagg gaaaaactta ttttgccatg atgctgaccg
    23221 cagcctgcac gaaccgtaag actttcccga acatggaaga gattgaacct tttaatgtga
    23281 tctatcagac tgcagaagac ggtatgggag atacgattaa gccgaggctg gtggaagcag
    23341 gagcagacct gagtcgggtg atggtcattg atgatacaga ggaagcattg acgttatccg
    23401 atgaccgtat tgaaaaagca atccgtcaga atcaggtcag acttttaatc attgatccgg
    23461 tacaggcttt tatcggtgcc gatgtggata tgaaccgggc aaatgaagtc cgtccggtat
    23521 tccggaaact gggaatgatc gctgaaaaga ccggttgtgc gattgtgctg atcggacatc
    23581 tgaataaatc ttcagggaca cagagtactt accggggact tggttctatt gatatcatgg
    23641 cggcggtgag aagcctgctt tttatcggga aagtaaagaa agacccgaca acaagagtac
    23701 tgatccatga gaaaagttct cttgcacctc caggtgagac gatggcattt aaacttggag
    23761 atgaagaagg attccgctgg gtgggagctt atgagatttc cgcagatgat cttctggacg
    23821 gaaaggaagg aaagccgaca gaaacaaaac tgcaacgggg aacgaaactg atctatgaac
    23881 tgcttgcaga cggaaatgca gtcacaatcc gggaactgga tgaaaaggca aaagcacagg
    23941 gaatctccca gagaaccatg cgagaagcca gaagccggat gaaagaagaa ctggattatc
    24001 ggatgaatga aaagcaggaa aataccattc gattaaaaaa acaagggaga atgggtgacg
    24061 gcagaatttt ggagtgaacg gcaggcttta tagataccct atctgccgtc tgccgtctgc
    24121 cggtgcaagg caatcaaacg gaaatagcag gtatccatac agaataagga ttgctgtgaa
    24181 tctggaacaa aaatgaattg taatatggcg gatataatgg tatgattatc cggtaacagc
    24241 aatgttacgg ggaagcacct gtgacagggt gtggctgcct cctgatattt acagaccgga
    24301 gcctttcggg gttctgagat gaaagaaagg aggtgcgtga tatgacagct tatgagatta
    24361 tctcaatatt cttaggcgta ctcagtttac tgatcgcttt tggtagcctg ttaattgcga
    24421 ttattgcctt tcttgatcgg aagaacaaga ggaaataaaa atgcctatcc tgtctgccaa
    24481 caggataggc ggtggtcgta agaccattta gtcaactcag gcggcatcca ctttggagtg
    24541 ggtgcttttc ctataacaaa tgtaccacac attcctcttg ttttcaatgg gaaaattaaa
    24601 atatgcagtt catctgacag ggatttatcc ctgtctttat tatatcccca atttctgaaa
    24661 tgttaataca aaaattttaa tatatgtaac caacagtctg ggcagttaat ctgtaaaaat
    24721 ggattagctg ctcttttttt gcaaaaaatg gaggttttac aatggcagtg aagaagaaaa
    24781 ttatcaacat cagagtatat tttgacggtg atcaggacgc aaaagaagtg tttgcagagg
    24841 tgatcgcaca gaaaatacgt gacacgaaaa caaaagataa acttgcaata atgaaagata
    24901 agcagtataa tgattatgag ttcagtaatc tgaaatctgc gtaggatgtg caaagtaacg
    24961 gaggtatcag atgaacaaca gatatatgag taacaatata gaaagaaatg caacctggag
    25021 agttggtgta tactgccgac tttccaaaga tgacgaattg accggagaga gtgccagcat
    25081 ctccaaccag agagacatac tggtgaatta ctgtcagtca cagggctggc aggtggtgga
    25141 tgtgtttcag gacgatggtt atacaggatt gaacacagac cgacctgatt tgcagagact
    25201 tctcaaagcc tgtgaaaaag gactggtgaa tcttgtaatt accaaagacc agagccgact
    25261 gggacgaaac catgtacaga caggatttct gatggaggag tttttcccaa agcatggagt
    25321 aaggtatatc gcattatatg ataatgtgga tacctttgcc ggagacaatg agattgcccc
    25381 atttaagaac gtgctgaacg agatgtattc cagagatatt tccaagaaag tccatgcgtc
    25441 ctatcacctg caggcaacca agggaaagtt tacaggtgtt gttccaccac tgggatatca
    25501 gaaagacccg gaggaaaagg gacatctcct gattgacgac gagacagcac cgattgtccg
    25561 gaaaattttc cagtgggcgg tggacggtca tgggattaac tttatatcca gagaactgga
    25621 acgccagcag attccctgcc cgtcatggtg gcaccggaaa cggggactcc gcacctatta
    25681 cacaaaatgg gaaaagaaag acccggaaaa tgggaaatat gtatgggatg aatcctatct
    25741 gaaatccctg ctgatgaatc ccgtttactg tggtgacatg gcttcccaga aaaggtacta
    25801 ccgtttcaag attggcaatc agggagataa agctccggag gactggatca cagtcaggga
    25861 tacccacgaa gcaatcattc cgcaggatgt atttgatctg gtacaggcaa agatgaaagg
    25921 caggaaacgc cagacagaac agggagaata cagcatgttt gccggattac tccgctgtgc
    25981 agagtgtggc ggtgcgttga cgttgaagaa gacgcacacc aaagaccagc atgaagttta
    26041 tacctgttcc acttatatcc ataaggggaa agcacattgt acccagcacc gggtggacgc
    26101 tgatgacctg tatgatgcag tccttaccag aatccaggaa tgtgcaaaag cagttaccgg
    26161 agacggaaca gaactggaaa accgggtaaa ggagttatgc gaggaagata cacagggaca
    26221 cagggaaagt ctggagaagc tggtatcaaa gcagaaagac cgcctggaaa cgttagaccg
    26281 gctgattgca aaactgtatg atgaccttat caatgacaag attacagaat ctgtttttga
    26341 taagatgctg gagaagacac agaaggagca gacagatatc aaaaaggaac ttttccagaa
    26401 tgagagtgtg ctgaatacgg aagaaaaact ggatgcacag tcccagcagt ggattgacga
    26461 tattagcgag tatgcggaca tcaaagaact ggatgccaat cttctgaacc ggctgatcag
    26521 taaaatcgtt atctcagaac cgcaggagaa agacggaaca gaaaattact gccgtactcc
    26581 tgaaacggtc acaatggaaa ttcatttcaa cttaaaacca ataccggagc ttggaaccat
    26641 cgaaagaggt tctggctccc ataaataaga aaaatctatc aaaatttaat ctattttaat
    26701 cttttcaagg ctcatttcga gcaaatattt tctcataaaa gacataaaac agcttgtcgc
    26761 tggcggcggc gtggccctga tcggtgtcac tctggttccc ctgctgtccg ggctgctggg
    26821 ctgatcctgg cctttgtccc tgtccaaacc cttaactaat cgcctccgcg tcctcccggc
    26881 cagaccggga gggcgcggga aaggaggtgc tacccgtgat tagtgaaatc attgaagaat
    26941 ggataaaagg catcctcatg gacggtatca cgggcaacct gtccgggctg ttcgatacgg
    27001 taaatgccaa agtcggagaa atcgccgccg acgtgggacg aaccccgcag gcatggaaca
    27061 gcggtatttt caatatgctg cacagcctgt cggaaaccgt gatcgtcccc atagccgggg
    27121 ccatactcgc ccttgtcatg tgctatgagc ttatccagat gataacggaa aagaacaata
    27181 tgcacgactt tgacagctcc atgttcttcc gctggatatt caaatccgcg tttgcaatcc
    27241 tgatcgtaac gaacacatgg aacatcgtca tgggcgtatt cgacgccacc caaagcgtcg
    27301 tcaatcaaag cgccggtgtg attatcgggc agacatccat tgattttaac accctgatac
    27361 cagaccttga aagccaattg gaggctatga gcgtgggcgc tcttttgggg ctgtggttcc
    27421 aaacccttgt tgtgggcctc accatgaata tcctttccat ctgcattttc cttgtgacct
    27481 atggccgcat ggtggaaatc tatgttgtca cgtcgctggg gcctatcccc cttgccacaa
    27541 tgggcaacgc ggaatggcgc ggcatgggcc aagggtatct gaaatccctg ctggcgctgg
    27601 gcttccaagc ctttttaatc atggttgtgg tgggtatcta cgcggtgctg atacagaata
    27661 tcggttccgc ttccgacata tccggcgcaa tctggggctg tatgggctat acggtgctgc
    27721 tgtgcttctg cctgttcaag accggcagta tatcaaaggc cgtattcggg gcgcattgat
    27781 cccaaaatcc aagcatacac aggaggaact tgtttatttc aacgtacaac atcatttacg
    27841 ctgacccgcc gtggcggtat gactgcaaaa acgtacaggg cgcagcggaa aatcactatt
    27901 ccaccatgag cattgacgag ctgtgcgccc tgccggtgga acggctggcg tccaaagact
    27961 gcctgctgtt tttgtgggcc acattcccca tgctgccgga agctctgcgg ttaatcaaag
    28021 cgtggggctt ttcattcaag accgtggctt ttgtatggct gaaacagaac cgtaaaagcc
    28081 ttacatggtt ttacgggctg ggccgctgga caaggggaaa cgcggaaatc tgcctgctgg
    28141 caacacacgg caagccgaag cgtcgttctg cctctgtcca tcagtttatt atcagcccga
    28201 ttgaacaaca cagcaaaaag ccggatgtga cccgtgaaaa aatcgtagag ctggcgggcg
    28261 atttgccccg cgtggagctg tttgccagac agaaaacccc cggctgggac gtgtggggga
    28321 acgaggtgga atgtgacctt atcctgacgt gaaaggaggt gccaaatggc ttatgtaacc
    28381 gtccccaagg acttaaccca tgtgaaaagc aaggttgtgt ttgggcttac caaacggcaa
    28441 ctgatctgtt tttccggggc gctggtgatc ggcgtcccct tatattttct cacccgtgac
    28501 tatcttacca acagcgcggc ggccctgctc atggtatttg ccatgctgcc gggctttttg
    28561 cttgccatgt acgagcgtca cggccagccc cttgaagtgg tgattatgca gatcatccag
    28621 tgctgcttca tccagccaaa ggagcgtccc tatcagaccg acaacaccta caccgccctt
    28681 gtgcggcaat accagatgga acaggaggta aaggccattg tccaaaaagg aaatcccaag
    28741 agaaaccgaa aaaacaaagc tgacccgcgc ccagcgaaaa gaaattgacg cggtaatccg
    28801 taaatataag ggcgacggca ggccccacac ggcgcagcag agcatcccct acgaggtcat
    28861 gtacccggac ggcgtgtgcc gggtatcgcc cggcgtcttt tccaagtgca ttgagtttgc
    28921 ggacatcagc tatcagcttg cccagccgga cacgcagacg gctatctttg aaaaactgtg
    28981 cgacctgtat aactatgtgg acgcctccat tcatatccag ttttctttcc tcaatcggaa
    29041 agttgacccg gtgcagtacg ccaagagctt tgagatcgcg ccgcaggggg acgacttcga
    29101 cgacatccgg gccgagtaca ccggcattct gcaaaagcag cttgcaaacg gcaacaacgg
    29161 catggtaaag accaaatacc tgacattcac catcgaggcc gaaagcgtga aagcggcgcg
    29221 agccagattg aagcgcatcg gctttgacct gttaggctat ttcaagagca tgggggccgt
    29281 agcccatgtg atggacggct gggaacggct gaacctgctg catggcgtgt accacccgga
    29341 cggcgagata ttcaattttg actggaagtg gctggccccg tccggcctct ccaccaagga
    29401 ttttattgcc ccgtcctccc tgtgcttcgg caatgcaaag acattcggca tgggcggcaa
    29461 atacggcgcg gtaagtttcc tgcaaatcct gtccccggag ctgtcggacg atatgctggc
    29521 cgacttcctc aatacggaaa gcggtgtttt agtcaatctc catgtgcagg ccattgaaca
    29581 gaccaaggcg atcaagacca tcaagcgcaa aattaccgac cttgacgcta tgaagattgc
    29641 ggagcagaaa aaggccgtcc gcagcggtta cgatatggac attcttccca gcgaccttgc
    29701 cacctacggc gaggacgcca agaagctgct gacaaagttg cagacccgca atgaacggct
    29761 gttccagttg acgttccttg tcctcaacgt ggccgacacc aagcaaaagc tgaataacga
    29821 cgtgttccaa gccgcaggcg tggcccaaaa gcataactgt ccccttgtcc ggctggacta
    29881 ccagcaggaa cagggccttg caagcagcct gccgctgggc gtcaatcaga tcaagataca
    29941 gcgcagcctt acgacttcca gcgtggccgt gttcgtcccc tttgtgacgc aggagctttt
    30001 tcagggcggc gcggccatgt attacggcat caacgccaag tcccgcaaca tgattatgct
    30061 ggacaggaag caggcccgtt gccccaatgc cttgaagctc ggcacccccg gcagcggcaa
    30121 atccatgagc tgtaaatccg agatcgtcag tgtgttctta acgacgcctg acgatatttt
    30181 tatttcagac ccggaggccg aatattaccc ccttgtcaaa cggctccacg gacaggtaat
    30241 ccggctgtcg cctaccagca aggactttgt gaacccgctg gacatcaacc tgaattattc
    30301 ggaggacgat aacccgctgg ccctgaaatc cgactttgtg ctgtcgttct gtgagcttgt
    30361 catgggcggc aagaacgggc tggaagccat tgaaaagacc gtgatagacc gggctgtgcg
    30421 cgtgatctac cggccctatc tggcagaccc ccgcccggag aatatgccga tcctgtccga
    30481 cctccacaaa gcgttgttag atcagcatgt cccggaggct gaccgggtgg cgcaggccct
    30541 tgacctgtat gtgtccggct ccctgaacgt atttaaccac cgtaccaacg tggacatcgg
    30601 caaccggctt gtgtcctttg acattaagga gctgggcaag cagctcaaaa agctggggat
    30661 gctcattgtc caagaccaga tatgggggcg cgtcacggcc aaccgaagcc aaggaaaggc
    30721 cacatggtat tttgccgacg agttccattt gcttttgaaa gaggaacaga cggcggccta
    30781 ttctgcggag atttggaagc ggtttaggaa atggggcggc attccaacag gtgccaccca
    30841 gaacgtcaaa gacctgcttt cctccccgga aattgaaaat atccttgaaa acagcgactt
    30901 cattacgctg ctcaatcagg cgtccgggga tagaaaaatc cttgcggaac gcttgaattt
    30961 gtccaccgaa cagcagaagt acatcgacaa ttccgaaccc ggcgagggcc ttttgatctt
    31021 tgaaaacgtg gtgctgccct ttaccaaccc catcccgcac aacacccagc tttacaagat
    31081 tatgacgacc cgcttaaacg aggtggcggg agtatgaccc ggcgacgcca caaaggaggt
    31141 gaaccaccat gaaagagctg aaagccaagg cgaaagtgac gcaggccatg acccgcgacg
    31201 ggctgacgct ggaaaatcag gccaccggcg aggtggagaa catttccagc cgggaggccg
    31261 aacaggattt gtccgcgccg gggccgggcg gcaccgccga taagctgctg gaacgggccg
    31321 acgccatcca tgacggccat aaggccaaga aagccgcgaa gcaggccggg gctgtggccg
    31381 ccgaggggga cgctgccctg caccggccat ccgcccggct ccaactgtcg gaggaagaac
    31441 gggccgaccc ggatttacag ccctatatcc gaaaatcgga caaacgggcc gataagctgg
    31501 ataaggcccg cgccgccatc cccaagaagc gcgtcccggt caaggaaacc gtctttgacg
    31561 aggcaagcgg caaagcaaag accgtcctgc actttgacaa acaggacaag gggcctccct
    31621 ccctgaaacc caatccggca ggccgtcctg tttccgaggt ttttctattc ggccacggga
    31681 aaatccatga agtcgagcat gaaaacgtgg gtgtggaggg cggccataag ggggaggaac
    31741 ttgccgagcg gcaggccgga aaagccatcc gcagcaccat ccggcaccac aggctgaaac
    31801 cctactggga cgcggaaaag gccgagcgca ggctattgtc tgccaacgcg gagtatttct
    31861 accagaaatc tttgcgggat aacccggaga tcgcacaggc ggccagcaac cccatttccc
    31921 ggttctggca gaaacagcag atcaagcgcc agtacgccaa ggccgcccgg acagcgggcc
    31981 agaccgcagg cgcggcggca cagggcgcgg ccaagacagc cggggcctcc acgaaagcgg
    32041 ccaaggccgc agccaaagaa agtgaacggg cggcctcctt tgtggcccgc cactggaaag
    32101 gggcgctggc cgtccttgtg ttggggcttt tgatgctgct tatcatcggc ggcttacagt
    32161 cctgcaccgc catgttcggc agcgccggaa gcggcatcac ggcgtcctct tacttttccg
    32221 aggatagcga tatgttggcc gccgaggacg cctacgcgga gctggaagcc gagctgcaag
    32281 actatctgga caactacgag gccacccatg actatgacga ataccatttt gaccttgacg
    32341 agatcagcca tgacccctat gtgctgattt ccattctgtc ggcgctccat gacgggatat
    32401 ttaccatcgg ggaggtacag ggtgatttgt ccatgctctt tgacaagcaa tatatcctga
    32461 cagaaaacgt cgtggtagaa acccgttacc gcaccgaaac cgatacatgg acggacgcgg
    32521 agggcaacac ccatacagac acctaccaag tgccgtatga ctattacatc tgctacgtca
    32581 cgctggaaaa cttcgacctg tcccatgtgc cagtctatat catggacgag gaaacgctaa
    32641 gtttctattc cctgtatatg tccacgctag gaaaccggcc tgatctgttt tccggcagcc
    32701 agtaccccaa cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt
    32761 tggaggatgc cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct
    32821 atgtgtgggg cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg
    32881 taatcaatca tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct
    32941 gcacccctgt ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct
    33001 acgacacccc cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt
    33061 gcgggaaccc gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca
    33121 gttacgggcg tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac
    33181 cgcatcgaaa aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt
    33241 cgggagctgg aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc
    33301 ggcatgaaca tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc
    33361 gcccctgccg caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag
    33421 acggatttcc cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc
    33481 cgttaccgcc cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac
    33541 cgaacccgct acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg
    33601 gacagaaacc gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct
    33661 gttttccgcg ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat
    33721 caccaccggc accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg
    33781 caggctgaat gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa
    33841 tgggacgacg gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga
    33901 gcgcatcggc tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac
    33961 cggcgagggc ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact
    34021 gttcaatggc ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga
    34081 cgacatcggc agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg
    34141 caatgtgttt tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt
    34201 aaatcaggtg gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc
    34261 cgctgctgtc tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc
    34321 ggtatgtaaa accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga
    34381 ggaacccgaa gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct
    34441 gctggtggcg ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa
    34501 ggctgaaacc accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt
    34561 ggaggacgag gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga
    34621 ggacgaggaa tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt
    34681 gcgggcgtga tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg
    34741 gtgagctggt gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt
    34801 ttcaaaaaat ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc
    34861 cccgacgaca agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg
    34921 accggccttg tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc
    34981 taccagatgg cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac
    35041 agtgctatta aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat
    35101 caatcggccc tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt
    35161 ttttccgtcc tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa
    35221 atgctggtgg acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc
    35281 catatcacgg cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca
    35341 aacaccgtca aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag
    35401 aaaaagacgg aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac
    35461 cggcttttcg gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag
    35521 aagctgctga cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc
    35581 gtggaaagcc ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt
    35641 tccccgcagt ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc
    35701 cccaccgctg aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg
    35761 ctgtccctga tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc
    35821 gtcaccgcca catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt
    35881 tcccccggct ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac
    35941 gaggacgccg aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac
    36001 gtggccgcct ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg
    36061 cttttgtccg ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag
    36121 ggattaggca caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt
    36181 gtccagagaa aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg
    36241 ctgccggagg cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag
    36301 atcgccaagg ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg
    36361 gagctggtgc agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag
    36421 cgtgttccta tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac
    36481 tactactgcg gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag
    36541 gaacgcaaga aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc
    36601 aaggtaaagg gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg
    36661 gctgataccg ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta
    36721 acacagcaag catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg
    36781 ccgggccggg gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa
    36841 cagagaaagg agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt
    36901 tttggaacag ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca
    36961 agccgccctg cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg
    37021 gtattcctgc ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga
    37081 cgtggacgag ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc
    37141 agagctgaac gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat
    37201 cgactacacc gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca
    37261 gttgggcgac tactacctga accgttccgg catggtggat atgccggagg aatggaaagc
    37321 cgggattgat acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac
    37381 ccagtacggc tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc
    37441 ggtgccggag gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc
    37501 gcaggcccag cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc
    37561 gatcatgctg gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga
    37621 aaccggcgta caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc
    37681 caagatgcac gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat
    37741 tgtggcgggc tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa
    37801 aggcattaag atactggccc cggccccgtt caaggttaag cagcagatgg agaaaatcga
    37861 cccggccacc cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga
    37921 agtgacggtt ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa
    37981 ggattttccc atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt
    38041 ctttgccgcg ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg
    38101 ggccaagggc cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga
    38161 attgcagacc ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga
    38221 ccgggaggcc cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca
    38281 ggccgaaagc gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc
    38341 ttttggctat gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct
    38401 tgaaaccatc cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca
    38461 gcttcagcag gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc
    38521 ggaggcagcg gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc
    38581 cgagccgctt gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat
    38641 gccgttgcat gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc
    38701 aggctatgac aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta
    38761 cgaggggcgg caggattttg gagataggga cggttccctg atcgaacata tccagagcta
    38821 ccaccaattt tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat
    38881 agagggctgg gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta
    38941 tatgaagctg cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc
    39001 cggggaaacc ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca
    39061 ggagtgccgc cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc
    39121 tgacttcgac cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga
    39181 ggccgccgca gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca
    39241 acaggacacc ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt
    39301 tgagccatac gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct
    39361 tgtttatacg gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa
    39421 catcgaccac ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct
    39481 gcaccagaat ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc
    39541 ggagtttttg cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa
    39601 catgattgac ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca
    39661 gctatctttt gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc
    39721 tgcgcccccg caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc
    39781 caaggaagcc atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca
    39841 ctatgtcgat caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta
    39901 tcacgaaaag atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa
    39961 taagggctat gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt
    40021 cccggtacgg gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca
    40081 gtacattcag ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga
    40141 cgatccgcag gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga
    40201 gttgatgaag gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca
    40261 tctgaccgaa agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga
    40321 gaaaaaaccg ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa
    40381 gggccgcatt gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga
    40441 cggcggcact gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa
    40501 gccggatgct gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc
    40561 cggggcatgg cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg
    40621 catccagccc ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa
    40681 agaaaagccc tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc
    40741 accggcgcgg gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt
    40801 ggacgttctg gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga
    40861 ctttggcatt gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa
    40921 gaccctgctc tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt
    40981 cctgaaagac acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa
    41041 gatactggcc tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct
    41101 ctatgagctg gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag
    41161 tgtgaaccgg ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga
    41221 cggaagccct gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc
    41281 ggctgtgctg caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg
    41341 cgatttcaaa gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg
    41401 tattgtggac agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat
    41461 ggtggagatc tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt
    41521 tgccatgctg ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta
    41581 tgccgtgatt aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat
    41641 ccgcaagcag ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac
    41701 caaaaagaat gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct
    41761 gatctgtatg taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat
    41821 gccggttatg gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg
    41881 catgacggat gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc
    41941 agacgccggg cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat
    42001 tcctacgcat ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc
    42061 aaacatgcgg gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca
    42121 gctataatta gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt
    42181 gtgcaggtga tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg
    42241 ttccagcggg aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc
    42301 ttcgtcaatg actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc
    42361 gttgatgtag tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac
    42421 ggcggctttg atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc
    42481 gtcagactga ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc
    42541 ttttctgctg gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca
    42601 cccacatacc ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct
    42661 aagtgcgtag gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc
    42721 cgtttggagc aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg
    42781 cactcacagc agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca
    42841 tccagcggga ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt
    42901 acgcaaatac attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag
    42961 cactcatgcg tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc
    43021 atagctcttg cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct
    43081 tgatgatgcc gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt
    43141 ccttttcctc ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt
    43201 tcacaccctg tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat
    43261 gctcacgccg gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga
    43321 gctgcttgta ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca
    43381 tagctttgag ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt
    43441 ccaaatccgg gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct
    43501 gcttcaaatt gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt
    43561 tgttttccag atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa
    43621 cgcggccttt gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc
    43681 agcgggtaaa gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct
    43741 catagccctg cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag
    43801 cggcgtcaat ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc
    43861 actcggcata gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct
    43921 tgcacagcct gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact
    43981 tgcgctgatt taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat
    44041 caatatgggt agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct
    44101 tcccgatctc atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt
    44161 ggtggcccag attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt
    44221 ccgctgtttc ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg
    44281 ggttctcgat gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa
    44341 ctgccatatc tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc
    44401 cataccgttc agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc
    44461 ggtctgcgcc cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt
    44521 ccgggcgtag gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc
    44581 ccgttcctcc ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct
    44641 tcaccgtcct ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc
    44701 cggcagggac ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt
    44761 tactgtttct ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc
    44821 tggaaagaaa aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa
    44881 acagaggatg ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg
    44941 gcgctgtcaa tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg
    45001 tggctgaccc tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt
    45061 tccacaggaa gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc
    45121 ccgtcgtttt cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct
    45181 ttgcgcgaaa cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag
    45241 ccgggacaat cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac
    45301 tctgcccgtg gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt
    45361 cttttattct tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca
    45421 tgacgttgaa agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt
    45481 gtgctgcggg acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa
    45541 ctgcggaaaa gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata
    45601 atgaaaccgg cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac
    45661 ggaaaaacta taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc
    45721 tgaaacagaa agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca
    45781 gaaatggaaa atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt
    45841 agaattgaaa aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact
    45901 gctggtctaa tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct
    45961 ttttgaggaa aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa
    46021 ggacacggat gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg
    46081 gtattaggac tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca
    46141 ggccctgcct gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat
    46201 tttgctgagg cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg
    46261 gtggatcaga atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg
    46321 ttttgacaac cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat
    46381 cttgatttaa ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta
    46441 tcccaatgac ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac
    46501 agactgttat gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg
    46561 tcgaggatga tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc
    46621 aggtggatgc ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc
    46681 aactggttat tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat
    46741 ttcgtgcgaa aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc
    46801 aaatccgggc gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga
    46861 ttttactaaa gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc
    46921 gtgtcggcag gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc
    46981 tgcccctgac acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa
    47041 ccttaacgca tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca
    47101 gcacagtcca cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa
    47161 ccattcgcgg tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt
    47221 cattaaggtt ttttcctata cgatcattgt cctgctactg gttgtcggtg taacagcaac
    47281 actgtttgca cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa
    47341 atcctatcag ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt
    47401 ggcagggctg tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg
    47461 aagcgtactc tatgccacac caaatgccaa tacatcaaat agttttagac ccgactttct
    47521 ttatgtggta catagagatg ataatatttc gattgttgct caaaacaagg caggtgtggg
    47581 attgctttat caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt
    47641 cagcctttta tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc
    47701 ggacagtgcg aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga
    47761 tgagcttggc gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat
    47821 cgcaaggctg gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt
    47881 ctttgcggca gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga
    47941 gggaatgctt ggaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat
    48001 caaaatgatg gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa
    48061 tgatgggaga atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt
    48121 gctgcccgat tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc
    48181 agccgggcaa attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat
    48241 attgaatgca gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg
    48301 tgctgaaaaa tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct
    48361 tccaaggctg ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg
    48421 aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc
    48481 gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt
    48541 gtaaatattt aaaacttaaa tgattttgac cgacaggtat aaccctgccg gtcttttttt
    48601 gctgctacag gaaaactaca gactggctac agggaaagta cagatgcgct tgccataata
    48661 acaatcgtac cagccacaaa tcgtagtttt attgcaaagg aggcattcga tcaaatggaa
    48721 aaaagcaact atcattccaa tgcggatcat cacaaacggc atatgaaaca atccgtggaa
    48781 aagcgggctt ttctatgtgc gttcattatt tcgttcacag tctgcacgct gtttttgggg
    48841 tggagactgg cttccgtatt ggaggcaaca cagataccgc ccatccctgc aactcataca
    48901 ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg ctcttgccac cgccaaagaa
    48961 cagggagatg aacaggaatg gagcctgatt ttagtgaaca ggcagaaccc catccccgca
    49021 cagtacgatg tggagcttga gcaactatca aatggtgagc ggatagatat tcggatttct
    49081 ccctatcttc aagatttgtt tgatgccgca agaactgatg gagtttaccc gattgtcgca
    49141 tccggatacc gaacaacaga aaaacagcaa gaaattatgg atgaaaaaat tgccgaatat
    49201 aaggcgaaag gctacacctc tgcacaggct aaagcggaag cagaaacttg ggtggccgtg
    49261 ccgggaacga gcgagcatca gcttggtctt gctgtggata tcaatgcgga cggaattcat
    49321 tcaacaggca acgaggttta tagatggctg gatgaaaaca gctatcgttt tggttttatt
    49381 cgtcgctacc caccagacaa gacagagata accggtgtga gcaacgagcc gtggcattac
    49441 cgatatgttg gcatcgaagc tgccacagag atgtacaacc aaggggtttg ccttgaggaa
    49501 tatttaaaac cagaaaaatg agaaaaggat gttatgttat ggacagaaaa agattgacac
    49561 agcgtttccc gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg
    49621 gaatgagatt tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt
    49681 tgctctttga aacggattgc gcattataca accacaatac cggatttgac atgatatacc
    49741 aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc ttattgataa
    49801 gaccggggga aaccttttct ttctggtggc tggtacgcca tgcggacaaa gataccccct
    49861 ataaagacgg ccttacggtg accaatggta aactcaccac catgtcgggc ggcggtatgt
    49921 gccagatgag caatttacta ttttggatgt tcctgcatac gccattgaca attatccagc
    49981 gcagaggtca cgaagtaaag gagtttccag agccaaacag tgacgagatt aaaggggtgg
    50041 atgcaaccat tccagagggc tggattgatt taaaagtgcg aaacgatacc gactgcacct
    50101 accaaatatg ggtgacctta gatgatgaga aaatcatcgg tcaggtgtcc gccgacaaag
    50161 agccccaagc attatacaaa attacaaatg gcagtatcca gtatgtccgt gaaagtggcg
    50221 ggatttatga atatgcccag gttaaacgga tgcaagttgc cttaggtacc ggggaaataa
    50281 tagattgcaa gctgctttat acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg
    50341 tggatattca ggaggagaac caatgagaaa aagtgtgggc attactgttt ttggatgcga
    50401 acaggatgag gcaaatgctt tccgcgcttt atcgccggat tttcatatta tccctacgct
    50461 gattagcgat gcgatatcgg cagacaacgc aaaattggcc gctggcaatc aatgcgttag
    50521 cgtaggccat aagtccgagg tttccgaggc gacaattctt gcgctgagaa aggtcggggt
    50581 aaaatacatt tctacccgca gcatcggctg cgatcacatt gatacgactg ccgccgagag
    50641 aatgggaatc tcggttggca cggttgcgta ttcgccggac agcgttgcgg attatgcttt
    50701 gatgctgatg ctgatggcca tacggggtgc aaaacccacc atgcacgccg tggcgcaaca
    50761 agatttcaga ttggatcgta tccgggggaa agaactgggg gatatgactg tgggagttat
    50821 tggaaccggc catatcgggc aagcggtcgt caaaaggctg cggggatttg gatgccatgt
    50881 gctggcctat gataacagcc gaaaaatgga tgcagattat gtccagcttg atgagcttct
    50941 aaaaaacagc gatattgtta cgctccatgt gccgctttgt gcggataccc gccatctgat
    51001 cggtcagaag caaattggag agatgaagca aggcgcattt ttaatcaaca ccgcgcgcgg
    51061 agcgcttgtc gataccgggg cgctggtgga ggcgttggaa agcggaaagc tgggtggtgc
    51121 ggcactggat gtgttggagg gcgaggatca gtttgtttat accgattgct cgcagaaagt
    51181 gcttgaccat ccctttttgt cgcagctttt aaggatgccg aatgtgatca tcacacccca
    51241 tacggcgtac tacactgagc gtgtgctgca ggatactaca gaaaaaacaa tcaggaattg
    51301 tctcaatttt gaaaggagtt tacagcatga atagaataaa agtcgcaatc atcttcggcg
    51361 gttgctcgga ggaacatgat gtgtcggtaa aatccgcaat agaaattgct gcgaacattg
    51421 atacggaaaa attcgatccg cactacatcg gaattacaaa aaacggtgta tggaagctat
    51481 gcaagaagcc atgtacggaa tgggaagccg acagtctccc cgccatactc tccccggata
    51541 ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg cgtattgatg
    51601 tggctttccc ggttttgcat ggcaaattcg gggaggatgg tgcgatacag gggctgtttg
    51661 tattgtctgg tatcccctat gtgggctgtg atattcaaag ctccgcagct tgcatggaca
    51721 aatcactggc ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa tttcaaatta
    51781 ttgataaagg tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt gtgaagccgg
    51841 cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa cttaacgctg
    51901 cgatagaagc ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg atttcgggct
    51961 gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt gattgtcggc gaagtggatc
    52021 aaatccggct gagccacggt atcttccgca tccatcagga aaacgagccg gaaaaaggct
    52081 cagaaaatgc gatgattaca gttcccgcag acattccggt cgaggaacga aatcgggtgc
    52141 aggaaacggc aaagaaagta tatcgggtgc ttggatgcag agggcttgcc cgtgttgatc
    52201 tttttttgca ggaggatggc ggcatcgttc taaatgaggt caataccatg cccggtttta
    52261 catcgtacag ccgctaccca cgtatggtgg ccgccgcagg aatcacgctt cctgcactga
    52321 ttgacagcct gattacattg gcgttaaaga ggtgataagt atggaaaatg gttttttgtt
    52381 tttagatgaa atgttgcatg gcgtccgttg ggatgccaag tacgccacat gggataactt
    52441 cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa aggccgtggc
    52501 gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt ggctatggct tgcttttatg
    52561 ggatggatat cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag cgcaaccgga
    52621 ggacaatctc acaaaagaaa aattttaccc caatatagag cgagccgagt tgattacaaa
    52681 gggttatgtg gcctcacaat ccagccatag ccgtggaagc gcaattgatc ttacgctcta
    52741 ccacctggat acaggggaac ttgtttcaat gggaagtaac ttcgatttta tggacgaacg
    52801 gtcgcatcat acagcaaaag ggatagggga tgcagaggca caaaatcgaa gatgcttgcg
    52861 taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc actataagtt
    52921 gattgatgag ccataccccg atacctattt taattttgcc gtttcataat gaaagtattg
    52981 gttttgtatc agattttgga tttgataaaa tctatgctgg tcatggacaa tcttttactg
    53041 gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta tagtattcaa tctgcccagc
    53101 ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc acgcccattt tgtattttgc
    53161 tggcggcggt tttgagaaat caaggccgcc gttcttttct ggcagcaaaa ggtgtggcgg
    53221 cccataggag gatgccgcta tgctgttttg ctattgccat gctctaatcc acttccacca
    53281 ctcaaaaaag ccttgcccct ccatgaactg aaccccaaaa gttgaactat ttaatggact
    53341 gtttccgata ttctactgga gatagtccat ttaactttag ttttatcctt ttgttattat
    53401 accacctaat atactcattt aatttacttt gaaatatctc aattgatcgg aatttttctc
    53461 gataaaaaaa ctctgattta agcacaccaa aaaaattttc tattacagaa ttatctaagc
    53521 aatttccttt tctagacatg ctttgaataa tgttattctc ttttaatttt ttttgatatt
    53581 gtggcatctg atactgccat ccttgatctg aatgtagaat cagtgaaccc tcagttccct
    53641 tttttttaat tgcttgttga agcatttctt caatcagttt atatgttgga ctagttgata
    53701 tactataact aattatttct ccgttaaata aatcaagtat aggagataga tagatttttc
    53761 ttccttttat cttgaattca gtgacgtctg tcacccattt tttattgggt gtatctactg
    53821 aaaaatttcg ttttaacaca ttcttggcaa tttttcctac tgttccttta taagatttat
    53881 atcgctttat tcggatttga caggtaagtc ccatttggga cattaatttt ctaactgttt
    53941 tatgattgat tgtatatcct ttcatcttta acgctaaagt gactctacga taaccataag
    54001 agtttcttga ttcttttaca atcgctgtaa tttcttgctt tatcttgcta tatttatctg
    54061 gcttatctaa tttttttacc cagtaataat aagttgactt cgctaattgt gcaattgaaa
    54121 gtaatagatt caatttaaat tcttttttga gctgaaggat tgttttaacc ttgatttctt
    54181 cttgctcaaa tcttgttctt gaatcaaggt ttct
//
LOCUS       DAJSVP010000012        52631 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSVP010000012 DAJSVP010000000
VERSION     DAJSVP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.012"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(69..1115)
                     /locus_tag="ON449_000706"
     CDS             complement(69..1115)
                     /locus_tag="ON449_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3933319.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(1126..1398)
                     /locus_tag="ON449_000707"
     CDS             complement(1126..1398)
                     /locus_tag="ON449_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3933320.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(1433..2341)
                     /locus_tag="ON449_000708"
     CDS             complement(1433..2341)
                     /locus_tag="ON449_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3933321.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(2473..3705)
                     /gene="pepT"
                     /locus_tag="ON449_000709"
     CDS             complement(2473..3705)
                     /gene="pepT"
                     /locus_tag="ON449_000709"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3933322.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(3720..4841)
                     /locus_tag="ON449_000710"
     CDS             complement(3720..4841)
                     /locus_tag="ON449_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3933323.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(4838..5539)
                     /locus_tag="ON449_000711"
     CDS             complement(4838..5539)
                     /locus_tag="ON449_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3933324.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(5683..6123)
                     /locus_tag="ON449_000712"
     CDS             complement(5683..6123)
                     /locus_tag="ON449_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3933325.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(6252..7178)
                     /gene="dnaI"
                     /locus_tag="ON449_000713"
     CDS             complement(6252..7178)
                     /gene="dnaI"
                     /locus_tag="ON449_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3933326.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(7178..8551)
                     /locus_tag="ON449_000714"
     CDS             complement(7178..8551)
                     /locus_tag="ON449_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3933327.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(8574..9065)
                     /gene="nrdR"
                     /locus_tag="ON449_000715"
     CDS             complement(8574..9065)
                     /gene="nrdR"
                     /locus_tag="ON449_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3933328.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(9377..9709)
                     /locus_tag="ON449_000716"
     CDS             complement(9377..9709)
                     /locus_tag="ON449_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3933329.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(9761..10390)
                     /locus_tag="ON449_000717"
     CDS             complement(9761..10390)
                     /locus_tag="ON449_000717"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3933330.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(10354..11190)
                     /gene="mutM"
                     /locus_tag="ON449_000718"
     CDS             complement(10354..11190)
                     /gene="mutM"
                     /locus_tag="ON449_000718"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3933331.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(11235..13880)
                     /gene="polA"
                     /locus_tag="ON449_000719"
     CDS             complement(11235..13880)
                     /gene="polA"
                     /locus_tag="ON449_000719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3933332.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(14006..14689)
                     /locus_tag="ON449_000720"
     CDS             complement(14006..14689)
                     /locus_tag="ON449_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3933333.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(14682..15167)
                     /locus_tag="ON449_000721"
     CDS             complement(14682..15167)
                     /locus_tag="ON449_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3933334.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(15474..16808)
                     /locus_tag="ON449_000722"
     CDS             complement(15474..16808)
                     /locus_tag="ON449_000722"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3933335.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(16939..17700)
                     /locus_tag="ON449_000723"
     CDS             complement(16939..17700)
                     /locus_tag="ON449_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3933336.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(17716..18198)
                     /locus_tag="ON449_000724"
     CDS             complement(17716..18198)
                     /locus_tag="ON449_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3933337.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(18218..20398)
                     /locus_tag="ON449_000725"
     CDS             complement(18218..20398)
                     /locus_tag="ON449_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3933338.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(20542..21273)
                     /locus_tag="ON449_000726"
     CDS             complement(20542..21273)
                     /locus_tag="ON449_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3933339.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(21342..22283)
                     /locus_tag="ON449_000727"
     CDS             complement(21342..22283)
                     /locus_tag="ON449_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3933340.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(22463..22831)
                     /locus_tag="ON449_000728"
     CDS             complement(22463..22831)
                     /locus_tag="ON449_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3933341.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(23442..24833)
                     /locus_tag="ON449_000729"
     CDS             complement(23442..24833)
                     /locus_tag="ON449_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3933342.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(25046..25432)
                     /locus_tag="ON449_000730"
     CDS             complement(25046..25432)
                     /locus_tag="ON449_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3933343.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(25488..26111)
                     /locus_tag="ON449_000731"
     CDS             complement(25488..26111)
                     /locus_tag="ON449_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3933344.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(26175..26843)
                     /locus_tag="ON449_000732"
     CDS             complement(26175..26843)
                     /locus_tag="ON449_000732"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3933345.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(26855..27295)
                     /locus_tag="ON449_000733"
     CDS             complement(26855..27295)
                     /locus_tag="ON449_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933346.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(27354..28025)
                     /locus_tag="ON449_000734"
     CDS             complement(27354..28025)
                     /locus_tag="ON449_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3933347.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(28210..28608)
                     /locus_tag="ON449_000735"
     CDS             complement(28210..28608)
                     /locus_tag="ON449_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3933348.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(28605..29378)
                     /locus_tag="ON449_000736"
     CDS             complement(28605..29378)
                     /locus_tag="ON449_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3933349.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(29375..30058)
                     /locus_tag="ON449_000737"
     CDS             complement(29375..30058)
                     /locus_tag="ON449_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3933350.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(30225..31112)
                     /locus_tag="ON449_000738"
     CDS             complement(30225..31112)
                     /locus_tag="ON449_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933351.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(31109..31978)
                     /locus_tag="ON449_000739"
     CDS             complement(31109..31978)
                     /locus_tag="ON449_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933352.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(32115..33401)
                     /locus_tag="ON449_000740"
     CDS             complement(32115..33401)
                     /locus_tag="ON449_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3933353.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            33674..34252
                     /locus_tag="ON449_000741"
     CDS             33674..34252
                     /locus_tag="ON449_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3933354.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            34252..35181
                     /locus_tag="ON449_000742"
     CDS             34252..35181
                     /locus_tag="ON449_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3933355.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            35178..36914
                     /locus_tag="ON449_000743"
     CDS             35178..36914
                     /locus_tag="ON449_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3933356.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(36977..37813)
                     /gene="pheA"
                     /locus_tag="ON449_000744"
     CDS             complement(36977..37813)
                     /gene="pheA"
                     /locus_tag="ON449_000744"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3933357.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(37810..38319)
                     /locus_tag="ON449_000745"
     CDS             complement(37810..38319)
                     /locus_tag="ON449_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3933358.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(38312..39607)
                     /gene="aroA"
                     /locus_tag="ON449_000746"
     CDS             complement(38312..39607)
                     /gene="aroA"
                     /locus_tag="ON449_000746"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3933359.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(39620..40711)
                     /locus_tag="ON449_000747"
     CDS             complement(39620..40711)
                     /locus_tag="ON449_000747"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3933360.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(40728..41894)
                     /gene="aroC"
                     /locus_tag="ON449_000748"
     CDS             complement(40728..41894)
                     /gene="aroC"
                     /locus_tag="ON449_000748"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3933361.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(41896..42960)
                     /gene="aroB"
                     /locus_tag="ON449_000749"
     CDS             complement(41896..42960)
                     /gene="aroB"
                     /locus_tag="ON449_000749"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3933362.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(42993..44015)
                     /gene="aroF"
                     /locus_tag="ON449_000750"
     CDS             complement(42993..44015)
                     /gene="aroF"
                     /locus_tag="ON449_000750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3933363.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(44674..45648)
                     /locus_tag="ON449_000751"
     CDS             complement(44674..45648)
                     /locus_tag="ON449_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3933364.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(45629..47512)
                     /locus_tag="ON449_000752"
     CDS             complement(45629..47512)
                     /locus_tag="ON449_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3933365.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(47530..49755)
                     /locus_tag="ON449_000753"
     CDS             complement(47530..49755)
                     /locus_tag="ON449_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3933366.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            49870..50904
                     /locus_tag="ON449_000754"
     CDS             49870..50904
                     /locus_tag="ON449_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933367.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(50971..51984)
                     /locus_tag="ON449_000755"
     CDS             complement(50971..51984)
                     /locus_tag="ON449_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3933368.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(52062..52250)
                     /locus_tag="ON449_000756"
     CDS             complement(52062..52250)
                     /locus_tag="ON449_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3933369.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT        14725 a        11453 c         9241 g        17212 t
ORIGIN      
        1 aacgtaaaat tatcttatcc ataacgtttt tctttgattt gataaaaagg gtagatctta
       61 taaaagacct acccttgatt ttctttattc tcttttttta cttctattat ttcttctttg
      121 atcactcttc gccgttcagc aatcacatac acaaatccat agagaaatgg caagataaag
      181 gaactgaaac gatacagtaa aagcgaagaa actgcgtcaa ctgttccaac aactggtctg
      241 aataataaca aatagacaaa ttcaaaggaa ccaataccag caggcgtagg aataacccct
      301 gaaagcacta ctgcaaagct gataaacgaa atggtcagta gaaaatctat tttaggatgg
      361 ttttcaatca gaatcaaata aggaagtaca taccaaaata caagttttaa catattccat
      421 agataaatcc gcaatagcgc agtcctgtct tggataatgg tttggaccgt ttcccttaaa
      481 gagtagatct gctgattgca tgtatccacc catttccgca aacgctcact tttaaataac
      541 ttattcgata agctgacaaa aaatatttga acattcaagc tgacggacat cgccaataaa
      601 aaggcaacaa tcacgatcgt caaaaggata ccagcgatga taaaccagat cattgatgga
      661 ccattagaat aaaaaagaga gaattggatg accaagccag ctaatgcata agtgatcact
      721 gccagtttgt acataatcat atggagtgct gtcacccctg ctccctggga tagcttcatt
      781 ccttttttgt tataaaaata aatctccgag atcagcgttc ctgtaccaaa cgaaacgatt
      841 cggtaaaacg caatatagct agaggtcaag aacccatctt tcgttgtaaa attcggcgcg
      901 aaaggttcag caatttcttt gattgagcgc ccttcagcga tttggtaaac tgtccctaat
      961 ataacagccg ctgccaaaac aacaacactg gtagacagca attcggcaaa gatatctgca
     1021 aatgattgat tgattaaata ataaataatg gcaaaaatca caatcagcaa aagcagattc
     1081 agaatgattt tagttttcgt tcctttagac gacatctcct gctccttaat ggaaaaaata
     1141 aatgagaatc aaatcaacaa gtacacctgc agcgaatcct atagtaaact tctttttccg
     1201 agttttatga tggaaaactt ttcgagagat caatcctccc aaaccaccgc ctaacaggct
     1261 gacaaatatc aaattattct caggaacacg ccagccgcta gttctagcct gatgtttatc
     1321 ataccccatc agacaaaata agtaaaaatt cacaatgaaa aggtaaaacc acaaaggatt
     1381 gtgtaacaat acatccatta ctcttcttcc aactttctgt taactactat ttctaagctt
     1441 tttcttcttt ctttggtgaa taaactgtta atccttttga tacaacagat aattcaactg
     1501 gaagatcatc tgtcgtgtct ccatccgttc gagtgcccac agatttttcc tgcgcttgga
     1561 ttttcatgcg tttggctgtg aaataagtaa tgccggggat cgtatagaat ttgcctctca
     1621 tgattctagg caaataaaaa ataaatcgga aaatatctaa ggaaggtacg atcgttacat
     1681 gaaattttcc atcatctagg gtcgctgagt cgtcaaaatt tgtaaaaccg cccacggagt
     1741 tagacatcgt gactgtcaac agttgggatt ttcctttcca atgctcctcg tctgtttcta
     1801 aatccaaatg atattttttc ttcttcagca ataacttaaa aaacttcttg ataaagatcc
     1861 acgagccgta acgctgtttt tcttcttgac tgatccaaac tgctgtatct gctaagattc
     1921 cgattgtcag tgtagagata atgacttcat cattgacttg accatagtct attttccgta
     1981 cattgccttc caggatcacg tcagcagctt cttctttttt gaggggaatc tcaagaacac
     2041 gtgcaaggtt gttgacagtt ccacctggaa gtaatccgac atggtattga gataatgttt
     2101 tttggaacgc ttggacgata tgatgaatcg tgccgtctcc acctataatt actaaagtat
     2161 cagcttctat ggcttctgct ttcttgacta attccgcatc atcaatcgat gggccagttt
     2221 cagctagttc tacatataat tctggctgct tttcttttac ataatcttga aaccagcgtg
     2281 ctaatttctc accctcatct ttccctgaac ttttgttata aaaaactagc agcttttcca
     2341 ttctttacct cctatttact cttcaaaaaa gagggcggga caaccaattc cattgtcctg
     2401 ccctttagcc ttagacagat gacattccat cattgggacg aacgccttgt cataagctgt
     2461 tctttttcga ttttaactct ttgtgttcaa ttctgcaatt tttacaatca cgtctgttgc
     2521 tttttccatt acttgtaaag aaacaaattc gaatcgtccg tgcatatttt ctcctccggc
     2581 aaatagattt ggcgtcggaa tacccatgta agagattttt gaaccatctg ttcctccacg
     2641 aactggttcg atcactggag agataccaag ctcgatcatt gcttgtttcg ctaattccac
     2701 gatgctcata tctttttcaa tgatttcttt catgttgtaa tattgatcat acatatctac
     2761 tttgattcgt tcttgatcaa atcgctgatt caactcttct tggatatgtg tgatttgtgc
     2821 tttgcgtgct tcgaattttt cacgatcatg atcgcgaatg atataactca tagaagcttc
     2881 ttcaggattc ccatccaatg ccatcaaatg gaagaaacct tcgtatcctt ccgttttttc
     2941 tggaacttca tcggctggaa gctgattatg aaaatcgatt gctaactgta gagcattgat
     3001 cattgtatct tttgccgtcc ctggatgtac atttttccct tggatcgtaa tatttgcttg
     3061 agcagcacta aacgtttcat actgcaactc acctaaagga ccaccatcga ctgtataagc
     3121 aaaatctaca tcgaattgct caacatcgaa tttatccgct ccgacaccga tttcttcatc
     3181 aggaccaaaa gccactttga tggtaccgtg agggatagaa ggattattga tcaagatctc
     3241 cattgcagtc atgatttccg caatccctga tttgtcatct gctcccagta atgtactgcc
     3301 atctgttgtg atcaatgtgt ggccttcata atttttcaaa tttggaaaat cttttgtatt
     3361 caatgtatag cgttcttccg catctagtac gatcgtagac tcgccatcat agttttcaat
     3421 gatttgtgga ttgactccta ctgcattaaa atctgctgta tccatgtggg aaataaatcc
     3481 aatggaacgc acttcatgat caacattgct tggtaatgtt cccataacga atccgttatc
     3541 ttcattatag aaaacatcag ctaagcctaa ctcttctaat tcttttttca attcttgcgc
     3601 aaaagcaact tgtgtctgag tcgatggagt cgttttgctt gttgcatcgg agcgtgtttc
     3661 tgttttgaca tatcgtaaaa aacgtggcaa aagattttcg tacatagtat tctctccttt
     3721 taattaaatt gaaatgggtt tgtatttgtt tcagaaacga aaaagttaat atcccaattt
     3781 tcttcttgtt tccaagattc aaatttctcg ataaatcgtt gcttgcataa cgattcaata
     3841 taatgaccgg gatcaattgc aatcaaccca gcgctttgca tatcatgagc cgtatgataa
     3901 tagatatctc ccgtgatata gacatctgct ctttgggcga ttgcttgcgg ataaaatttt
     3961 tcaccgcttc caccgcaaat cgcaatgcgc ttcacagagg attttgcatt ttttggctgg
     4021 actattctca gtccatctag ttggaacgct tcttttactt gttctacaaa tgcctctata
     4081 gaaatttctt gcggcaattc acctactcgt cctaacccaa acatttcgac tggttcatct
     4141 atagcgaaaa gatcataagc aggttcctca taaggatgag ctgaccgcat ggcttgaatc
     4201 acttgttttt cgatagtttc cggcaaaata acctcaactt ttgcctcttg aacttgttct
     4261 gtcttcccta ctttcccaat cgctggttgt gcaccagctt ccggagtaaa tcgaccgtgt
     4321 ccaatcgagg taaaacttgt accggtataa tcaccttgtg taccagcacc agctgcagct
     4381 aatacttcac gcattttctg tgcatgatcc acaggtacat aaacggccag ttttttataa
     4441 tgaatctcat gagtctttac taaataactc tccacttcaa taccgagaag ttcacaaaac
     4501 cagtcattca gtccatccca aataatatcc atgttcgtgt gtgctgcata gacggcaata
     4561 tcatgtttca acaaatctgc atacattttt tcttgtggct gatcagtaac cagccgtttt
     4621 acaggacgaa agattggagg atgtttagca atcagcaaat cgattttttt cttgattgct
     4681 tcttcgacca cttctggacg aacgtctaat gtcatcatta ctcgttggat tggcttatcc
     4741 aatgttccga tatgcagtcc gactgggtct ccttcttcag ctagccactg aggacaatag
     4801 ctttcaaact tttggatgaa agtgcgtccg ctgattgtca tggctttaaa acctcctcaa
     4861 tcatttggat ttcctgctcg atttgttttt gacgtgcctc ttgattagct gcttgtttga
     4921 gctgttcacg gatagctttt ctttgttgaa gctctcttgt ccattttgat ttgaagatct
     4981 cacttttctc ttcaagcaga aatggcccga acatcagctc catttcattg taagtaactg
     5041 gtttttgttc tttttcggct acaatgattt catatatttt tttattttct tcaagtattt
     5101 tttctgattg gattttatac tgattttctt gcagccaaga tcgtaatgta cgctctccga
     5161 tgtttggctg aaggatcaaa cgttcttgtc cgcttaagcg ttgattcaac ttgccagctt
     5221 ctaggatatc tcggatcagt gaacctccca tacccgcaat cgtgacagct gtaataccat
     5281 cttgtggctg gatagcatct agaccatttg caaggcgtac ggtgatacgg tcttctaatc
     5341 catctttttt gacttgctta cgtgctgatt cataaggacc ttgaacgact tcaccagcaa
     5401 ctgcatattc gatttttcct cttaatatca atgcaacggg taagtaagcg tgatctgatc
     5461 caatgtctgc taaacgagcg gattcaggga caaagtcacc cactgcggct aagcgctgag
     5521 ataaatctaa atggttcata gcttcactcc ttctagataa ttatagcatt ttccaaataa
     5581 aataaggaga ctaggaataa aaaaattccc atatctcctt aaaaaatata ccttttcgtt
     5641 ctataaaaca acattttctt atttttgaaa aaatgttatt ggttatttgt tcaatgaaac
     5701 atcaataatc gaatctccag gaacttgcgg tgggatagac atcgtcatga tcggcatctc
     5761 aactaatgta aattggactt tgtcaccgat tttcaattca tttatgtcca cgcttatttt
     5821 ttcagcagta gcatttaaga tcactccgtc attgctgaat gacgttccca tattcgctgg
     5881 atcttctttg gccatgacat cgacgagttt caactgaagc gtttcttctt ctgcttctac
     5941 ttgagtgata ctttcaatcg ttccgacaaa tatgctttgt ctctccgttt tcgtactgct
     6001 tgtttttact gtttgacccg catcaatcga atcaacagaa cctgtgttgc tatctgcgct
     6061 accgcagcct gctaaaagaa aaaatagacc aactacagat atactagaaa aaatagccct
     6121 cataaaaaat cctccgttta agtttctgca ttacttgttg cggtgattgt ctccggtcat
     6181 ttctgtccat ataatgattc gctgacctgc ttttactact aggtcaaatg tcagttggac
     6241 ttgtcaactg ttcaaccatt gcgtcggttg cgaccagtca tttcgatttc ttttgtcaaa
     6301 taacgtatgc gttccattat ccgtttcgct tttagcggct cttcatctcc acgttgagtg
     6361 acggacaaat gctgttccaa ttcatccatt gtaaaattcg atgagaagaa ggttggtaac
     6421 tgttcctgca tccgatattg aaggataaca ccgaatactt cgtctcgtat ccaactactc
     6481 atcgcatctg caccaatgtc atcgatcatc aaaataggtg aacgtttcac tgcatctaac
     6541 ttttctgcca cttgatcttt tccgattgcc tgtttcatct caacggcaaa tgtcggaaaa
     6601 tggactagcg tcgtggtgaa tccagcaatc gctaattctc tcgctgttgc tcctaataaa
     6661 tatgtttttc caataccaaa acttcctact agatacagtc ctttatgata cccttttgga
     6721 tcagcagtat atttctctac aaaatctagg gcttctaagc tcgcatatcc tcgtccatct
     6781 gtacgttcaa agttttcaaa agaagccgat tgaatatctt ttggcatatc cattgctttt
     6841 actcggcttc tgatttcttc ttgtctttgc cgtgctaaca atgcttccgt tggtacataa
     6901 gtgacatcca cataatgaaa attcaacgtc agcttcggct catagccggg agcgatcatc
     6961 gttggatcgt ttttttgata tttacgcttt tcttgcacga attcatacaa tttagcgtaa
     7021 cttttttcaa tatccgcttg tgtcaattct tcttggtgtg cgcttagaaa agcttgaaca
     7081 tccgcatcat ttaagacttc agcgatcatt tcagcatact tttcttgata attgcgtttg
     7141 ctgatgatcc tcttcaattc tttgcctacg tcttccacta gatatcaccc tccttttttg
     7201 ccaggtattc tttaaaacgg cggtcaatct ctgctttttt ctcgctgctg atctgttttt
     7261 catcttttgg ctggttgacc caatcaggaa gcttttcccg tcgaacattt cgcttgccag
     7321 tttgataatt tgtttgcttc ttttgttttg attgttttgc ttgattactt atttgccgaa
     7381 tgtgctggat cgcttcttct gctgtggcga ttttcttttg tgcccattca tttgccactg
     7441 tatttacata actggcattt aagctagcat tattcttgat cactagtaca taattcagca
     7501 aaacattgat cacgctactg gaaagacctg attgagaaac aagatcttgc agtaaccagt
     7561 tttcttgttt agaaacatat ccgccttttt ctgacttgat tgcttccaga tatttcatcg
     7621 gtggaatgct ctgtgcttct tttatcagct gaagcgctgc gtttgtcaaa tgttcgtttg
     7681 acgagggttc attactgttt tcagaaaccg caactgtttc ttttgtttgc ttcaccggac
     7741 gtctctgcat tgccaaaatg gctttttgca gttctttttc agatactttt tgttcactaa
     7801 agtcataaga ttgactgatc agttcgccaa gtgtcaattc atcgaaaccg taaagctgat
     7861 gatacatgtc gacttgtttg atgattggat catcaagact tgcaatatgt ttttttgcca
     7921 gccaatcatt caaaaactgc caatcgaatg tcgaatctgc ttcaagtaat gctactgatt
     7981 cagattcttc aaaagattta ttcagcgatt cgatgttttc ttgattagca gcgtacactg
     8041 cttcattgaa gcgataaaca tctaaaaatt tttttgtgat tttttgatat ccctctgctg
     8101 tatgcctttt aggttcaaag aattgggtaa gcttctggaa tcgtcgttca ccaacttttt
     8161 ccagaagaag aaaagccaga acagagtctt tgaaaaatgc tttaggaatg agtggttcta
     8221 acagctcata aataaaacga ggtcccagat cactatcttc tttaaaataa acatccagta
     8281 agccgattcc ttctagcttt tttcttgcat cataaaattg cgggataccg cagcctagac
     8341 tactaagcag atctgcatgc atcccttcag gacctttgcc agattcttga gagatttctg
     8401 ataacagggt gaaatagata ctcaaggctt ctccgccgat tagtggctga taaagataca
     8461 gcaaggcgtt cttgctgtct tctctcagcg gagccgcttt tcttccttgg taaatatttt
     8521 ttggttgaag ctctttccaa gcattttcca aggaaatgcc tcctttttgt ttcttaattg
     8581 ttttctttat tattctcttt attattttct tttgctttat ccacgatatc ttgcaactct
     8641 tttaagaaaa cagacatatc tttgaactgg cgataaacac ttgcaaaacg aatataagcg
     8701 atttcgtcta aatcaactaa atcttccatc acatattcac caattagatt cgtcgatact
     8761 tcgttttctc cgagactgcg tacgcggttt tcgacatggt tgacgatttg ttccatttgt
     8821 tccatagcta caggtctttt ttctgctgaa cggatgagtc ctcttaaaat tttatcgcga
     8881 ttgaactctt cgcgttcccc gtttttcttg attactaata acggagcagc ttcaattcgt
     8941 tcaaatgtag tgaatcggaa cgaacaattc tcacattcgc gacggcgacg gatcgcacgt
     9001 ccatcatctg cttgacggct gtcgattact cgtgaattat tatgttgaca tctgggacat
     9061 ctcattttgt ctcacctctt gcatcgcatt ttatataaag gatgtgtctt tctttacaac
     9121 tagttgtatt ataacacgaa acaaatcaca aacgcgactg tcctacccaa aaaacacgga
     9181 tataagaaca attttttcta ctttcatgcg taattgtgtc ccctgtcctt actaaatttc
     9241 aattttccaa tactgatcac cttgattact gagaaataac ttacacaaaa gctgatttag
     9301 tcatctagca caaaaaactt gaagcagatg aacaccttct catctgcttc aagtcacatc
     9361 tcgatttttt actattttat aacttctctt cgattcctgt atagtgtttc attcgctgtt
     9421 cctcgatttt ttgctcatcg ggaatgtggt cactttctgg gagcggtgtt ttactacgat
     9481 caggtgtcgg attttttcgt tcttgagtca aatcttgccg cttcgcttct tcttctgcct
     9541 gctggtcttc tatttcatcg attcttgctt ggtactgctt ttctttttct tctgctgttt
     9601 ctgattgcgt acgaccttca tcgattagat tttctttatt gagcatctcg ccgatttttt
     9661 cttttgcgtt acctacgact ttatcctttt gattttttaa tgatcccatc gctatccctc
     9721 actttctata tactcaggat agcaaaattg acacagatac tcaaagaaag cgattagcaa
     9781 aaagccaatt ttcgatttgt cccacaagtt tttcttttgt tccattgttg tcgaacaaaa
     9841 tatccgcacg ctgctttttt tcttcgattg gaagttgact actaattctc tgctgcgctt
     9901 gttctagcgt cagttgatta cgtttcatca aacgctgaag ctgagtttct ggcgtagtat
     9961 aaacgacagc tactgcatcc atatagtggt cataatgacc ttcgtagagc aatgggatat
    10021 ctacaattac taaaggatga ttggaactta gagaggcgat ttgatctttt atcgcttttc
    10081 ggataaacgg atctaatagc tgatcaagtt tttgccgttt ttcctttgat ggaaaaacaa
    10141 tcatcccaag tttccctcga tctagttgcc catcaggctg caatacctct tggccaaaca
    10201 ctgatacaat tttcctcaga gcaagcgttc caggctcaac agtttctctg gcaatcacat
    10261 cgccatcgat caaaggaaat ccatgagact gaaagacttt ggctgccgta cttttaccgg
    10321 tagctattcc acccgtcaaa cctaaaacaa atcctacttt gcgattttta gcctttgaca
    10381 attcggacaa tagtgcgtcc ccctttgtgc cacttttgtt ttcacgatcg gcgttccaca
    10441 acgtatacat ggtttgccag tttgctgata gacatgcagt gctacttgaa atccgcctga
    10501 ttctcctaat gcattcaaat aactgcgaat cgtggtacct ccggcttcta ttgcacgatc
    10561 caaaacatca atgatcgata gacgtaattg ttctacctct ttggggcgca gtgtgttcgc
    10621 cggttgttct ggatggatct tcgcttccca taatgcttca tctacataaa tattacctag
    10681 accagtcact agacgttggt ctagcagcaa tggcttgatt gctttgtggg actttttcag
    10741 tccatttgcg aaatctgcta ataagaagct atctggcaaa ggctcaggtc ccaatttcat
    10801 gattcctcgg taagttgcac ttgctccttt ttcgacgatt gtcatacgac caaattttcg
    10861 tacatcgttg tagcgaagtt ggcttccatc ctcaaaaaag aaaagtacat gcgtatgttt
    10921 atcgattggt gtattttctt tcgtaaattg atattttcct tccattcgca agtgtgagat
    10981 cagttcacaa tgatcgagat gaaaaattaa aaacttccct ctacgtccaa tggattggat
    11041 ggtttcgcca acaagagaag cctcaaagat gggtgtctct ggttgctcga tgatcttggg
    11101 ccacaagacc tgaacttttt gaatcgtttt tcctactact aaccgttcca atcctttacg
    11161 aacggtttct acttctggta attctggcat tttttctttt caccttcatt atattgctct
    11221 gtcttaaaca atgtttattt tgcttcgtac cacgtttttc cccaactgct gtcagtgatc
    11281 agtggtacat gaagagaaac agcttgttcc ataacttcct tcaccaattt atctaatgat
    11341 tcaagttctt tttccggtac ctcgaagaca agttcatcat gaacttgtaa cagcatcgta
    11401 gcttgaagac ctgtttcttt taatcgttta tccaattcga tcatcgcgat tttcaaaata
    11461 tcagcagcac ttccttggat tggtgtattg atagctgttc gttccgcaaa agaacggata
    11521 ttgtaatttc ttgaattgat gtctggcaga tatcgacggc ggtgatacag cgtttcgacg
    11581 tagccttggt cttttgcttc gcgtacgatt ctttccatat attctttgac acctgggtat
    11641 ttttcaaagt acgtatcgat gtattgctgt gctgcttttc ggctgattcc tagattttgt
    11701 gaaagtccgt aatcagagat gccatacacg ataccaaaat tcactgcttt tgcctgccta
    11761 cgcatatttg gtgtgacatc ctctgccttt tcgattccaa atacacgcat cgctgtgctg
    11821 gcatggatat cttgaccttc taggaaggca gctttcaaat gttcatcatt tgagatatgc
    11881 gcaagtacgc gtaactcgat ttgtgaatag tcagaagaat aaatcagcca gtctttttct
    11941 ctaggaacaa atgcctgacg tatctttctc ccttcatcta atcgaatcgg gatgttctgc
    12001 aaattaggat caacagagct taatcgtcct gtttgggtca atgtctggac ataacgggta
    12061 tgcacttttc catctgattg gatcactttc agcagtccct cgacataggt agattgaatt
    12121 tttgcaattt gacgataggt gaggatatcc tctacgattg gtgcttgttc acgaagctgt
    12181 tccagtacat ctacggctgt ggaatatcca gtttttgttt ttttgataac gggtaatccc
    12241 attttttcaa ataagatcac tcctaattgc tttggtgaat tcaaattgaa ttcttcgccg
    12301 gcttcttcgt agattttttg ttcgatctct cttaagcgag cagagaactc gcccttcatt
    12361 tcttgtaagc gagtcgcatc tactttgatc ccgcttattt ccatctctgc caaaatgata
    12421 gacaatggca gttccatttt ccggaaaaga tcttcttggt ttttttctgc cagttcttgg
    12481 ctcaattttg gtgtcaacgc attgattgca gctacttttc gcgctaaatg agcaaaaaag
    12541 agttcctctt cttctggtaa accttttttc gcccctttgc cgtatacagc ttcatctgat
    12601 tggatatcgt tgtatccgta atgagcagct attccttcga tatctgtgct tttttcattc
    12661 gtatcgagaa ggtatgcacc tagcaagaca tcaaaatcga ttcctttcgt ttttccagta
    12721 tagcggttta aagcaacata agttcgtttc gcatcatata cttttttcag ccgagtcgga
    12781 tcagtgatcc aagaatggaa cgctcggttt tcgaatacag atagatcgtt cgtgacataa
    12841 attttctttt ctgtccccca cgctactccg acgatttcct ctgtatgata attgtcgccc
    12901 atcatttcca cgtacaacgc catatccgta gtaaacattt cctctttgaa ttcatgaacg
    12961 acttcaaaca aaatatcatc catttttact tcttcttcta cgatattcaa tttagaaagg
    13021 aactgattga aatccatttc tttataaaac ggaacgagtt tatcgagatt tttcccttca
    13081 tacttcaatg agtcgataga gacatccaca ggcgctgaag tattgatggt cgccaatttt
    13141 ttcgataaga aagcctgctc tttgtcattt atcagatttt ctttcatttt gctttgcttc
    13201 atttcatcga tatgttcata aattccttcg actgagccat attgtttcaa tagcttgata
    13261 gctgtttttt caccgatttt agtcacaccg gggatattgt cagaggcatc tccggctagt
    13321 cccttcatgt caataatctg ttttggcgtc aacccatcat atttttccgc tacatgttct
    13381 ggtgtatagc tttcgatatc gctgacacct tttacagtga tatccacttt tacttcttta
    13441 ctagctaatt gagtcaaatc ccgatcacct gaaagcacga cgacatcaaa ttgatctttg
    13501 tctacttttt ctgccaaagt accgataata tcatctgctt cataattggg caattcgtaa
    13561 tactgtaccc ctagtcctgt caacaaatca cgtatatatg gcatttgttc tttgaattcg
    13621 ccaggtgttt tggatcttcc acctttgtac tccgcataca tttctgtccg aaaagtcgtt
    13681 tttcctgcat cgaacgcaac cagaacatga gtcggttgct ctttttgcat aacattttca
    13741 aacatcgtat tgaatgcata gattgcattt gtatgaagac cgtttctatt tttgaaacgt
    13801 tcaagagaat tgtgaagcgc aaagaatgcg cgaaaagcca cactgttccc atcaacgagt
    13861 aataatttat tttttgtcat cttcttaact ccttactgta aagttcttat tcattctaac
    13921 aaagaatcct gcggaatgcg aaaagaaaaa gcgggaccat accaaataat tcggtatagc
    13981 cccaactgat gaattttttt cttttttagt tgttccgtcc aaagattctc agaaacgcca
    14041 agaacaagtt gataaagtcc aaatacagct tcaatgccat gaatacagca attccactat
    14101 gtgctgttcc gtttgtctgg acatagatat tacggatgct ttggttatca taagctgtaa
    14161 tccctgcaaa gatgacgact aacagtaggg agatgaacag ctcaactacg ctacttctca
    14221 aaatgaagat gttcaagact agtgcaatga tcactccgat taaagctgcc tgtcctgctc
    14281 ttccaacccc gctaagatct ctttttgttg ttactccgac aacagacatc cccatgaaca
    14341 tcaaagtagc tgtcaagaaa gcttgcgtta ctgttccaat atcgtagtat gcaagagtaa
    14401 ccgctaatgt tatgccattc aaaagagagt aggcaataaa tccaccgatt gctaagctcg
    14461 gattcttttg tgcttttacg cctagaacca ttactaaaat cagttcaacg atccacagac
    14521 ccacaaagcc tagtggaaaa ttattgataa aagttgctaa ttgcaaagga aatacccaaa
    14581 gagccaagta agaagtgact gcactaaggg caagtcccat acctaaaaat ccataaactt
    14641 ttgcataaaa acgattgata cctgacattg atgtacttgg attattcatt caattcacct
    14701 tcactttcga ttgctatttc caattcagga acttgtggcg gttggaccat aacgacagca
    14761 tccaacacac gatcttcgtt cgtattctcc gcttctacag aaatcgtcac cacatccttc
    14821 atcttatcta cttttatgac ctcaaactga aaagtcagtg tttcataatg gaaaaccggt
    14881 tcaataaaat tgacagaaaa attgacaaca tgtgatcctg gtccaggaag atgctttgaa
    14941 attgcacttg tgatgatccc catcagcatc actgatggaa cgattggctg ttgatattct
    15001 gttttttgtg caaagtcatg ttggatatac aaaggatttg catcattcgt caatcccaaa
    15061 tacagcaaaa gctggttatc ttcaatagat tctgttaagg ataacgagtc gccttcctcg
    15121 atttcattta ttgtttttcc tagttttctc ggcttaccta tgttcaagat agaaaccctc
    15181 cagtttattt ttaatcatac attattgtac caaaagtagc gtaaaaggca agttctgctc
    15241 ccttgattta acagattcct atagtttaca gaggtcgtga aaaagctgtc ctgcatcaaa
    15301 taataagaac agccaagaaa aaacagcttc tcatgttttt gatttgattg ggcttattac
    15361 cgcagatgca agcttttgaa caccgtttat cataagttgc aaaaaagggg gcttggcaag
    15421 atttttgcca ctccccctct ttctattttt ataaggacac tgcagtaatt gtattacagt
    15481 acatttttcg tcgtgctgct taaaagtttt tcatacgctt gttcaaattt ttgaacatct
    15541 ccagcaccca tgaagattac tactgcatca tgataatcta acaatggaga tacattattt
    15601 tctttgatga cttctccacc ttttttgatt ttcgctccaa ggtcttcaat cttaacgttt
    15661 ccttgttctt cccgagccga accaaaaata tcgcataagt aaactttatc agctaaatca
    15721 agtgcttctg caaattcatc catcaatgca atcgtacgcg taaacgtatg gggttggaaa
    15781 actgcaatta tttctttatc tggatatttt tggcgtgcgc catcaatcgt tgctttgatc
    15841 tctgcagggt gatgcgcata atcatcgact acggtcatgt ctgctacgat cttttcactg
    15901 aatcgacgtt ttactcctgg gaaagtcagc atttcttctg caacttcttt cagatccagt
    15961 ttttcgaaat aagctactgc aataacacct aaagcattta agatattgtg tttaccgaat
    16021 gctggtacag tgaagtgccc aacaaattca tccccatgat agacatcaaa agcagaacca
    16081 gaagttgtgc gttcgatgtt tcgtgcttgg atatcatcgt tttctgtgac accataataa
    16141 taaatcggca catttgcttt taatttgcgt aaataagcat cgtcaccata cgcaaaaata
    16201 gcttttttca cttgtccagc cattgtttgg aaagctgtaa atacatcgtc gatacttgta
    16261 taataatccg gatgatcaaa atcgatattg gtcatgattg catagtctgg tgaataggca
    16321 aggaaatgac ggcgatattc acaggcctca aatgaaaaga attccgcttg cggatcacca
    16381 tgtcctgttc catctccaat tagataactt gttggacgga caccactcaa tacatgagag
    16441 agcagtccag tcgtacttgt ttttccgtga gagcctgtta cagcgatact cgtataattt
    16501 tggataaaat gaccaataaa gtcgtgatag cgaatcactt ccaaacctaa ttcttttgct
    16561 cgctggattt cttcatgtga atcaggaaat gcatttcctg caatgatcgt catccctggt
    16621 tttacattat ctgcgttaaa tggcagaatc gtgatatttg ctttttctaa atctctttgt
    16681 gtaaagaaat atttttcaat gtctgatcct tggacattta gaccttgttc atgtaaaaca
    16741 agagccaagg agctcattcc tgaacctttg ataccaacaa aatgatacaa tttattttga
    16801 ttttccattt gaatcctctt ctcttatcta gcgtccattc ttttagctat tacattcaac
    16861 acgctcctca ttatcttata aacgattgaa aatttcaacc aaatttattt ggaaaggagg
    16921 attcgcttgc taaaatggtc aagtacgaga aaaatccgtt tttattgact ctttaattct
    16981 ggtgtaagaa gaccaaactt cctctgatat atcaatgtga ggaataaaat cttttccttt
    17041 cggaccatag ccgttctcat cgtcatggag acgttccatt tctcctaata ctagcgcatg
    17101 gaaatattcc ttaggccatt gatgattcag ttcttttgct gtaaagatga tctcttcaga
    17161 aaagtgaagg atttccggca caaatggcac agcattcgcg aaaatagttt gttcattttg
    17221 ccatactttc tcaaaagttg ctcgagtcct gcgttgcagt gtcggatcat tcatcaataa
    17281 gattttttct ggtacagctc ctaaggaatg aagaatctcc agtgaaaaag tggcgttctc
    17341 tccagaattt gtagacttcg attctatcag aaaagcctta tcgggcaaat catagacttc
    17401 ttttagatac tgacgacaga tttcactttc actcatgcca tcttcaaaat gaaatccttg
    17461 cttttcaaaa ttctcataaa ggattctagt cgcatgcccc acccctccga ccaaaaacaa
    17521 ttgatctact tggccattta aatacatttg tgctgcttta tcagctaaga taggaagaca
    17581 gtttccagct aaaataagtg taggtgccgt cagttcctca aaatttccct cttcaggtgc
    17641 ggctaaaaaa tttaatagct gattccagtc ttttagattc ctctgctcac ttttttccaa
    17701 aggccttcta tcctttcatc tgattttttg tgcttcatta aagtcttctc tttttgtctc
    17761 aatccatttg atttctccat gacatcttcc acaacgatat ttgcgtacat cgattcttct
    17821 cttgcggcag atcgtggcag aacaatcttg gcatcgataa tactccatct ttatctgttt
    17881 cttcgtcaag gctggtgcat agcgcaaccc tcctgttttt ttcaaaagat acttgaaatc
    17941 catatcttta tgctgatacc ctcttccagc tagatgaaga tgataatggc aaagttcatg
    18001 tttgatgatt cctgcaatcg tctcttcgtc tgactcttca aatagcttcg cattaaaatc
    18061 taaatgatga tcgtttaaat gatagcgccc gccagttgta cgtaagcgtg gattgaacat
    18121 tgcttggtgc ttgaaaggaa gctggaagct ttttaatgat atctcttcta ctaactgctg
    18181 cagttcttct tgtgtcaatt tttgttactc ctcattgtta ctcttcataa ggggaaagca
    18241 tcgtcaaact gattctacct tttttcgtat cgacttgttc tatccaaact gttacgatat
    18301 ctcctactga cacgacatct gtaggatgct tgacgaattt tttgctgagt ttcgatatat
    18361 ggacaagtcc gtcttgcttc acaccgatat caacaaatgc gccgaagtca atgacattac
    18421 ggaccgttcc agtcaattcc atccctggtt tcaagtcttc catactcaat acatctgtac
    18481 gtaataaggg cgctggcatc tcatcacgca tatctcgtcc aggttgtgtc agcccagcta
    18541 aaatatcggc taacgtttct tctccgactg ataggctttc agccagtttc tctacggata
    18601 atcgtgtcaa tttttctact gcttcttctg taccaagctc tttttctgaa agttggactg
    18661 aagttaatat ttcttttgca atagaataac tttcgggatg gatacctgtg ttgtccaaaa
    18721 tgtttttccc accagggact cgcaagaatc cgattgcttg ttcatatgct tttggtccta
    18781 agcgtggcac ttttttcaat tgagttcgtg cggtgaattc tccgttttct tcacgataaa
    18841 tgacaatgtt ttgggcagtt gttttattca atcctgatat atgctgcaag agctgcgggc
    18901 ttgctgtatt cacatccaca cctacttggt taaccgctgt ttccacaaca aaatccaatt
    18961 gttcagccaa acgtttttga gaaacgtcat gttggtactg gcctactccg acagctttag
    19021 gatcgatttt gactaattca gctaaaggat cttgcaggcg acgggcaata gaaacagcac
    19081 ttctttcttc aacttgtaga tgcggaaatt cttttcgtgc aatttcgcta gcggagtaaa
    19141 cagaagcgcc tgcttcgttg acgatagcgt aataggtttt atggtcggcg gatttcaact
    19201 gctcagccac aaaaagttca gattcccgac tggctgtacc atttccgata gctaccatat
    19261 ctacttgata tttattaatt aattggataa aagctggtcc agcagcttct ctttttgctt
    19321 gcgctgcagg tttatgagga taaataactt cgattgctaa aactttccct gtcgcatcta
    19381 cgactgctag tttacatcca gtacgataag ccggatcaaa tcccaggaca actttacctt
    19441 ttagtggcgg ttgcaacaaa agattacgga ggttttcgcc gaaaatagcg attgcttgct
    19501 catctgcctt ttcagtcaat tcattgcgca gttctcgctc gattgctggc tgaataaagc
    19561 gtttataact atcctgatag gcttctctga cgaatgaaga agaaggagag gcaggatttt
    19621 tgactatttg gcgatccaag taagctaaaa ttgctgcttc atctacttga aagaatactt
    19681 tcagaatatc ttctttttct ccacgattag tagctaatat tcgatgagaa accattttat
    19741 ggacgggttc tgcaaagtca taatacattt catatacgcc tttttcatct gcttgctcat
    19801 ccttgacatt gctgacatac atacctttgt tatacgtata agaccgaatc caagtccgaa
    19861 attttgcatt atcgctgact tgttcagcaa taatctcatg agcaccgtgc aacgcttctt
    19921 cggcagaaga gacttctttt tctttgtcaa tatatttttc tgcttcagat tgcacctctc
    19981 catctgtaag ctgcatcagc cacttcgcga gtggttcaag tcctttttct ttagcaatcg
    20041 ttgcttttgt ccgtcgtttt tgtttgtatg gacgatacaa atcttccact tgttgcattt
    20101 tcacggcttg ggtgatttct gcttctaact cgggagtcag tttcccttgt tcatcgatta
    20161 gccgaatcac ttcagtttta cgtttttcta agttttcaag ataggcatac cgctcttcga
    20221 tttcacgaat ctggacttca tcaagacttc cagtcatttc ttttcggtaa cgcgcgataa
    20281 aaggaacagt gtttccttca tttaacaaat taagtacagt agtcaactgc ttaggacgat
    20341 agtctgaaag ttctttttgt acaagctgaa tgatcgtctg gttcaaattt tccgtcatta
    20401 aaatgcctct tttctgctaa aaattgcttc tttattctat catacttttt atcctgggtt
    20461 aaactactga aagaaacaat attctctata aaaataaaaa gagccgcaac aaagtcacag
    20521 ctccttcatt ctttccatta gtcagattcc atgccaaggt ccttcgttgt tctgatattc
    20581 aatatcttga agaatacctt ccttgtcaaa aacaacccca tgcaatgacc ctccgaatac
    20641 agccccaccg tccattccga ttttatgatc ctgaatccag aggtctgttg tctgcatatc
    20701 tccatggagc attggtgtaa tcgtatgtcc aaatacaatt gtcttacctg tattgttttt
    20761 cccttcatga aatggttccc ttatccaaat aaagtctctt agacttgtct tacgccagtc
    20821 tttctttgtc aaatccacac cggcatggac aaaaagataa ttttcccatt cataatacaa
    20881 ggggcgctgg actaaaaatt taatcagttc tgaatatctc gattggatca tcatcgagat
    20941 ttcagttggg gagtattcct ccgatgcccc tgggtgaatc aggctttcaa acgtttcttt
    21001 tccaccattt ctcatataag gcgcatacca gtcctccggt gattgcagaa attgcaaaaa
    21061 atattcttca tggtttcccc ggaggtaaat ggctcctgtt tcttcgacta attgctttcc
    21121 taataaaaaa cattcttttg tacgaggacc tcgatcattg agatccccta ataaaacaag
    21181 ctgatggatc tttggatcat aatagtgaat aagtttttca aaaagctcat gtttgccatg
    21241 gacatcccca atagcataaa tcaatgattt catgtggatc accatcctca acgctagtat
    21301 agcgcatatt ggaaatggtc gacaatcaaa actattgttt tctatttaat taaagctgta
    21361 aaccgcagtt ctgcttcttc tggagagatt ccatggtctt gaacatagcg attgcgcgga
    21421 tgtttcgcac actctatgct acatgctcct aaatgttttt cttcattttc aatggaagca
    21481 agaatctgac gattgcactc aggattgcta caattgatat atcgttcaca tggtgttccg
    21541 tcaaaccagt cgcgaccgac gatttgttta tctacttggt tgatatcgac agcgatccgt
    21601 tcatcaaaca catacatctt gccgtcccat aattctcctt ggacttcagg atccttacca
    21661 taagtagcaa taccgccatg cagctgtccg acatctttga atccttcacg gacaagccaa
    21721 ccagaaaact tctcgcaacg aatgccacca gtacagtagg tcactacccg tttttccata
    21781 aattcttctt tgtgctcgcg gatccagtct ggaagctctc gaaaactgcg tatatctggg
    21841 cgtacagccc ctcgaaaatg tcccaagtca tattcataat cattccgtgc atcaatcacc
    21901 actgtctgtt catctaaaat agcttcttta aattcttgcg gtgaaagata ggcacctgtc
    21961 acttggtttg gatcaatgtc ctcttctagc tttaaactta caagctctgg acggtatctt
    22021 acaaacatct tcttaaaggc atgtccttcg cttggatcga tcttgaacat cgtatcagca
    22081 aatcgaggat cagctttcat tgctttcata taggcagtcg tgttttcaac ggttccagac
    22141 acagtaccat ttattccttc ttcagctact agaatacgac ctttaagatc caaagatttg
    22201 caaaaggcta aatgattttt ggcaaattcc tctggttctt cgatttttgt ataattgtaa
    22261 taaagcagta cttgataatc catgtatttc ttccttcctt cttttcgttc ctaaacagta
    22321 taaagaaggc gttcgcattt gacgatttat ttgcttgtgt ataattacgc atggaaagtt
    22381 ttttgtttac ataatattat tatttgaatt aaaaagactt cgctttcctg tttcgcacag
    22441 aaaaacaaag tctttatttc tattatttac ctgtaaccgc aaagtagttg ccatcgggat
    22501 cagcaaagtt aaagacatat tcttctccaa gctgtaccat atcacctaat gtgactgatt
    22561 gcgtctgcat ttttttatat agtccaaaga tatctttact gtaaaacatc aaagatgggg
    22621 cactagtagc tgtgccaggt gaatgggact caacaaatgc tcgatcatat aacagaaatg
    22681 aggtttgact gtcctcaact cctacttcaa ttataagcgt cccatccatt tcttgacgat
    22741 ctttttccgc aaagccaact gctctccaaa acgttgcatt tttttctaca tcgtccacat
    22801 aaagcatgat ttttatttca ttcgtaaaca tctgtttcct cctaaacgat ctgttctaac
    22861 attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag ctctcaacag
    22921 aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt ttttgttatt
    22981 tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata aaaaaagagg
    23041 tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca actcctcggt
    23101 cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct ctttgtgact
    23161 cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    23221 cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg ctgttcttat
    23281 tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag aagtagcttc
    23341 tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa acttcttctt
    23401 atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt cacatcgatc
    23461 actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc acttgtcacg
    23521 ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc gattaatggt
    23581 tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc taaaacgatc
    23641 agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact gattgttaca
    23701 attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc tgctggtgcg
    23761 acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc aatagacaaa
    23821 ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt tgtctgagtc
    23881 tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa gctcaatacc
    23941 ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa aatcagtgag
    24001 tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt acctaaattg
    24061 acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa tactccataa
    24121 ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag actgtcgact
    24181 aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag ataagcgatt
    24241 ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact acctgctaaa
    24301 tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga taagttttca
    24361 actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc tccttgtgtg
    24421 aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa tgctgcaata
    24481 gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag cttttcagaa
    24541 actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat aactagcatt
    24601 tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga ttgagtaacg
    24661 actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat accaactcct
    24721 aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg atagaacaca
    24781 tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt catttttctc
    24841 ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga gaatgattct
    24901 tcaaccatcc tcctgaaaaa atttttttca gtgactttta tattttagac gatttttatc
    24961 gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg atagctttaa
    25021 tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa ttcaatttta
    25081 ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt acgatatcga
    25141 ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga ccttcaattt
    25201 tccgattgag gatatgattg atctgactca cacttccaga taaaacatat gtatcaaaca
    25261 ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag ttttcgatcg
    25321 tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg ttcggcaatc
    25381 tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc atataatttt
    25441 atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta ctttttaatg
    25501 atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc cgggatctca
    25561 cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt ccctgcttcg
    25621 atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg aaaagtatcg
    25681 atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc gatccctact
    25741 acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt tgattctgtc
    25801 aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc cagtacttgt
    25861 ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg gtgaaataat
    25921 tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt
    25981 ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc
    26041 catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata
    26101 taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta
    26161 gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta
    26221 tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct
    26281 tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc
    26341 cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc
    26401 atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca
    26461 aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact
    26521 tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa
    26581 tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct
    26641 agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat
    26701 tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct
    26761 gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca
    26821 tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac
    26881 cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat
    26941 gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt
    27001 tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct
    27061 catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga
    27121 cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa
    27181 gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa
    27241 gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat
    27301 ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg
    27361 gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc
    27421 actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc
    27481 ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga
    27541 aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc
    27601 agattccgtt ttttactgat cacttttaaa aagcttttta gaatcttttg ctgacgtcta
    27661 aaaatagaaa aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct
    27721 gcagtcagat ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca
    27781 accactgctc gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc
    27841 aattctacct taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct
    27901 aatgttagcc cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc
    27961 aattgaacat atagaaatgt agatcgctca atcagagtat cttttaaaac aactaaaaaa
    28021 ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct
    28081 gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata
    28141 caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca
    28201 aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat
    28261 tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat
    28321 actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat
    28381 tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat
    28441 gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt
    28501 tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc
    28561 aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc
    28621 aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat
    28681 cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg
    28741 tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata
    28801 aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag
    28861 atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa
    28921 taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca
    28981 attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc
    29041 tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag
    29101 ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt
    29161 cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt
    29221 atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata
    29281 ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg
    29341 atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca
    29401 tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat
    29461 ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag
    29521 ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg
    29581 tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata
    29641 gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga
    29701 tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca
    29761 aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga
    29821 ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca
    29881 tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac
    29941 caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga
    30001 caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt
    30061 atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt
    30121 aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt
    30181 tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg
    30241 atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta
    30301 gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc acccatagca
    30361 gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa
    30421 taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc
    30481 gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct
    30541 tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca
    30601 gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc
    30661 aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat
    30721 agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat
    30781 cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa
    30841 ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc
    30901 aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc
    30961 aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac
    31021 tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt
    31081 tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga
    31141 ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa
    31201 aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat
    31261 ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg
    31321 tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa
    31381 actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat
    31441 tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa
    31501 gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca
    31561 taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga
    31621 tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc
    31681 ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca
    31741 tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg
    31801 catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg acagggggac
    31861 cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca
    31921 aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc
    31981 ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt
    32041 aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac
    32101 ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt
    32161 accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca
    32221 attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa
    32281 tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca
    32341 ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct
    32401 tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct
    32461 ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct
    32521 ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc
    32581 gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt
    32641 actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac
    32701 aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca
    32761 gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga
    32821 ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac
    32881 tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc
    32941 gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct
    33001 actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa
    33061 ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag
    33121 acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg
    33181 atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt
    33241 tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt
    33301 tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc
    33361 gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc
    33421 atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt
    33481 attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga
    33541 caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata
    33601 aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa
    33661 ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga
    33721 tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag ggactttttc
    33781 cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga
    33841 tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc
    33901 tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt
    33961 taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa
    34021 ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa
    34081 tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt
    34141 gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca
    34201 ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc
    34261 gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc
    34321 aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt
    34381 ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc
    34441 caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct
    34501 tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta
    34561 acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg
    34621 acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc
    34681 tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact ttctttgctc
    34741 aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt
    34801 gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc
    34861 gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta
    34921 caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa
    34981 aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc
    35041 tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa
    35101 ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt
    35161 aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc
    35221 tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc
    35281 atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa
    35341 agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg
    35401 attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc
    35461 ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga
    35521 tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc
    35581 tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac
    35641 gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag
    35701 cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg
    35761 ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg
    35821 agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa
    35881 atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt
    35941 caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc
    36001 aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata
    36061 atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa
    36121 aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta
    36181 ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc
    36241 taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc
    36301 ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat acgctgacta
    36361 gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt
    36421 tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag
    36481 aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct
    36541 cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc
    36601 aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca
    36661 cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg
    36721 gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc
    36781 atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag
    36841 tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag
    36901 aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa
    36961 tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca
    37021 atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca
    37081 ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg
    37141 gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat
    37201 gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg
    37261 actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat
    37321 tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag
    37381 caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg
    37441 attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg
    37501 ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct
    37561 gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa
    37621 gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat
    37681 cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt
    37741 gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat
    37801 agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt
    37861 ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc
    37921 caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc
    37981 atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg
    38041 ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat
    38101 aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt
    38161 ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt
    38221 atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt
    38281 tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa
    38341 gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta
    38401 gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat
    38461 cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt
    38521 ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc
    38581 tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt
    38641 gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac
    38701 caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt
    38761 cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag
    38821 ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc
    38881 ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag
    38941 ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa
    39001 attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg
    39061 tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag
    39121 gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact
    39181 ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc
    39241 ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag
    39301 ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg
    39361 ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt
    39421 cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca
    39481 aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg ctgcgatggg
    39541 aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata
    39601 attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga
    39661 ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa
    39721 tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca
    39781 gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa
    39841 aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat
    39901 tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca
    39961 tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat
    40021 ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg
    40081 ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag
    40141 ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg
    40201 taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt
    40261 ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg
    40321 ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat
    40381 ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga
    40441 cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg
    40501 gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt
    40561 aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa
    40621 ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc
    40681 caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc
    40741 aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat
    40801 tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct
    40861 gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg
    40921 atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg
    40981 acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg
    41041 aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa
    41101 ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct
    41161 tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga
    41221 acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga
    41281 tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct
    41341 tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca
    41401 ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat tttcttggca
    41461 attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc
    41521 cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga
    41581 cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt
    41641 gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg
    41701 ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct
    41761 ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt
    41821 aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca
    41881 gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg
    41941 aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc
    42001 tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat
    42061 cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa
    42121 gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg
    42181 actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt
    42241 taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc
    42301 caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc
    42361 gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat
    42421 tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc
    42481 agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt
    42541 cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat
    42601 cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag
    42661 tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc
    42721 tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa
    42781 attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc
    42841 agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc
    42901 tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat
    42961 tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt
    43021 gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat
    43081 tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct
    43141 gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca
    43201 tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt
    43261 aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg
    43321 acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta
    43381 ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt
    43441 ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata
    43501 tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct
    43561 tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt
    43621 ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat
    43681 tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg
    43741 tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa
    43801 gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat
    43861 gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct
    43921 tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt
    43981 tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta
    44041 cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact
    44101 aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata
    44161 atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc
    44221 cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg
    44281 taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac
    44341 gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag
    44401 ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc tagtatagac
    44461 ataaaaaaat cgactgtcaa tcatttttat gaaacttttt ttaattttct gatagttaac
    44521 tttgtttttc agaaaaataa caattggttt tattgaagaa aatagtcatg aacaaaagta
    44581 tctgagaact tttttactct cttgttcatg acatatagaa taaagcagct ttgtcttgta
    44641 aactccgttt ttcaaataat aatctaaaga ttgctactga ataataaagc cgatttcatg
    44701 gtcctcttcg ctgctgattc ggtaaggtga ctcaacatcc gctccccagc tattgatacc
    44761 cccgactcct cttacttttg caaaaatcgt aagaaccgtt cgtctttctg gcggtagttc
    44821 ttctatatgt gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg aaaaagcaaa
    44881 tggcgtatcc tgctgttcaa atcgcaacga aaaatcttct tgatctttat ccgtattatc
    44941 caacgtttga tttcttgtta gttctaccca tttcgtttcc atatgcatgc cgcattcttg
    45001 gggaacaagg tatggggaaa cggggagtcc tgccacttta taaatacctt cgacaccacc
    45061 agccatccga tccggatatg tttcccctga taggccttca tactcaaaaa attttgcttt
    45121 cgttggcata atgaacctta aacccaatgc gggtaattcc ggtaatcctt tttgtccata
    45181 ataatgcata ttgacaagca ttttaccctc tccagcgatt tggtaggaaa ttgtcacttg
    45241 ggtttttggt actgtgttga tttccagtgt aaaaatgatt tcaaccgttt cagcgtattc
    45301 attacttgaa tattgattat ttactggagc aatcggcaaa tcgatctttt tattgtccac
    45361 cagtacttct atttctttga cttttggata caatccggct cctaaccaca taccggattt
    45421 aaatccaaat ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa ctggaagtgt
    45481 ttcccggtat agccattctt ttccgttttt attgagcgat tccagtccgc ctcttgtata
    45541 agaaaaaata taatgcgtat tccccgattt gacacctaat gtacagtcgc cgaagacgat
    45601 tgcaactttt tccgtatcca tatgactttt atttactgta tttttcataa taatatctta
    45661 cctcctgcat ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc cctgagaatt
    45721 catagtcact tggtttatcg tcaaagtcac cgccatagcg catcacttct ctgcctgtga
    45781 ttggatcagt cacttggatc gcctgatctt taaaatccca tatatagcct ccttggtaca
    45841 tttcataacg atcaagcaac gtcatgtaag aatccattcc acctaacgaa ttccccatgt
    45901 catgcatata ttcacataag atgaacggtt tttccgcttt attttcaagg tagtcaatga
    45961 tctcttctgg tggcgcatac atgcagcttt ttacatctga aatcgaatct tcgaatgctg
    46021 gatttggaaa attcccttca taatggacaa gtcgatcggg gtctactttt ttgaagtaat
    46081 cgttcatttg tcgaatgttt tcttccgcat aagattcatt tccaagagac caaaaaagaa
    46141 tacttggatg gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga acagccgcct
    46201 gccactgagg tactgcacct ggtacgttgt aagaaggctc aaatgcgccc attttttgcc
    46261 atgagccatg ggattcgagg ttcgtttccg ccatcacatg gattcctgct tggtcgcata
    46321 aataatacca agtgatctgg ttggggtaat gagatgtccg tacgccgttg atctttgcat
    46381 tttttatgat agccaaatct ttttccatat cttctatagt gatggcgcgt ccttttttcg
    46441 gatgccactc atgtcgattt accccgttga taatcatccg ttttccattg aagtagatga
    46501 ttttatcttt gatttcgatt ttacgaaaac cgaaggaata gtcaatatac tccgttactc
    46561 ttttttcttc gtctatcgtc tccaattcaa gcttatataa attgggctga aaatgccccc
    46621 acagtgccac attttcaaat ccatcgatgc gaatcgtcat ttgctctttt aacggatatt
    46681 gctgttgata gatttccttc ccatcaggat ctagtatcgc tacattcagc tgactgtttt
    46741 tggctacttg ctcaaaagac aaatctactt caagtttgcc attttgacat ttgtcatcta
    46801 agcgaggttt gatccaaaga tcttctaaat ggttttgtcc tcgagcaact aagaagacat
    46861 ctctgaagat cccaaaaaat cggaaaaagt cctgatcttc caagaatgca gctgtacttc
    46921 ttttgtaaac ctccacagct agaatatttt cttcttccaa ataaggtgtt aaatcaaatt
    46981 cagacggagt gaacgaatct tcggcatacc cgataaaatg accattcaac caaataaaca
    47041 ttgcttgttc tacaccagca aaatgaatcg agatttgttt tccctttaga ccttcttcta
    47101 atcgaaaagt cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa aagatccctt
    47161 ccgcctcttt ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt ccctcccacg
    47221 gatacatcgt attgatgtaa tggatcttgt cataatttgc taattcaata tgactaggaa
    47281 cagtgatttc atcaaaagaa caggcatcaa aatctttttt ataaaatgtt tttggtcgta
    47341 ccgaaggatt tggcgaataa caaaaacgcc atgatccatt taaagactgt ttaagggagg
    47401 atgtggtttc tacttcttcc cttgttgcat aacaaacatg atcgctatgt gcatttgatt
    47461 ggttcactct aaaaacttgt ggatcatcta accattttat atttgctttc atactttttt
    47521 ccttctctct cataactttt tgaaatgaag cagttctgtt tggaaatcac cttgaaaatc
    47581 ggggaagtaa agtccaactg tcatcaattc ggcccccgaa aatatccgct tcgaggtctg
    47641 ttcctgataa agtgcctcat catctaaccc catcagatat acttcatgga acgctggttg
    47701 agctgaagct agtatcctcc ctaaaaacac aatagcttca tcttgtttag gagacacaaa
    47761 catccatgcg gcaatatttt cttcaaaagg actcgccaaa cgataataat ggccatactg
    47821 gatcaccgga cgaatcgttt gataggatat aatttggtct tttacttgct ttttttcttc
    47881 aggagacagc tttgtcaaat ccagttcata accaaaaaca gcgctcatag ctgtttctcc
    47941 tcttgttttc aacgaagtag atctgtcggt ctgatgattt ggtacagctg atacatgagc
    48001 tgtgatggac gaaatcggat aagcaaggct tgttccatat tggattttca tacgctccac
    48061 tgcatccgtg ttatcacttg tccatgactg cggcatataa taaataaacc cggcatcaaa
    48121 ccgtccacct ccaccagaac agccttccca taagatttcc ggataatcag ttgtcaaatg
    48181 ttcaagtagt tcataaagcc cgagcatata acgatgcgct acttctcctt gtctctgtgg
    48241 gtcaagggta atcgaataga catctgaaag cgaacggttc atatcccatt tgatgtagtc
    48301 aagaggaatc gtatctaaaa tctttctcat ttgtttctca atcgtctgcc gaacatctaa
    48361 ccttgtaaaa tccagtacat gttgtgatct ggaagcagaa ggcattctac ctggctcctg
    48421 cataagaaaa tcaggatgtg ttcgatacag ttcagaatca accgagatca tttcaggttc
    48481 aaaccataaa ccaaatttca atcctttgct atgagcataa tctgctattt cacgtaatcc
    48541 atttgtcaac ttgccttcat actcgaacca gtctcctaat gaagaattgt ccgcatctct
    48601 ttttccaaac cagccatcat ctaatacgaa catttcgatg cctagctcct ttgcttcatc
    48661 aatgatcgcc ttgatttttt cactattgaa gtcaaagtaa gtggcttccc agttattgac
    48721 cagaatcggc cgttcagcaa attgatgacg acctcttgcg attctttcac gtagcaactc
    48781 atggtggatc tgactcattt tgttcaatcc ctgacttgag taagaaagga tcatttcagg
    48841 tgtttgaaaa gactggcctg cattcaattc ccacgaaaaa cgatggctgt tgatcccacc
    48901 gacgactctc aattgattga tctgatcttt ttcaagttcg aaagaatggt tgccggaata
    48961 aacaaattga agtccgatcg cttctccagt attttctgtc gtatgatgat gaactaaagc
    49021 aataaagtta ttcatatggt gactgctcgt tcctcgacgg ctttcaaata cttttgttcc
    49081 agataaaaca gattgccgtg aaatctgtct ttcattgaca tgtgctcctg gcaaagcaat
    49141 gacttcatca aatcgcctag tatgggcaaa gtctagctgg aaactagccg ctttttctag
    49201 tttgattgtt tctccggttt catttcttat ctttacagat cgtgtgatca ctgctctatc
    49261 agtaaatatg gtatagaaca aatcgaaata aatcgctgtt tctctatcct taagtgaaat
    49321 caccaacgtt tgtgcctctg acttgtccat cacataagtt tgaggcaagc cttcgatctc
    49381 tggcttccca tctgtaatat aatggctgtc atatctcaga tccagtaagt ctgatccatt
    49441 cttgcgctga atgatcgatg cgggcgttcg aaagtccatg ctgccatgag aagagtattc
    49501 ttgcggtagt gtatctttcg acagagaccg gtcaggattt aagggaacat ttcctgaaaa
    49561 tccacgatct cttcggggat aatttttatt gtctttgtac tgcttgactg gtttgccaaa
    49621 ataaatatgc gccaatacat tgctttcttc tatctttatc agataagaaa gaaatgtatt
    49681 tcgtaaatgg aagactcggc tcttttcatc aaataagatg aaatttttat ctgtttttct
    49741 atgcgtaata tccatcattt cactcccaac aaaacggaaa ccgtttactt ttttttataa
    49801 ttcctgtata ctgtttacta aacaattttg tcaataaaaa ttttttattt ttttgaggag
    49861 tgtgtaaaaa tggttggaat tagagatgtc gcacgaaagg caaatgtctc tcctgctaca
    49921 gtttcccgtg tgttgaataa cgatcagacg atccatgtgg ctcagactac tcggcaacgg
    49981 atagtagagg cagctgctgc gttggattat gacatcagta ctcgtaaata tatcaaaaag
    50041 agaatgcctt cgattggtgt gatctctacg atcggcaaag cgagcgaaga acaggatatc
    50101 tattacaaac atttgcgagt agggatggaa gaagaagctc gacgtctgca tttagggatg
    50161 aatcgaatct ataatctatc tgataatcct aaacaatggg aagatctaga tcagttaggt
    50221 gcggtgatcg ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt aaaacaaaac
    50281 atgcaaatag tcgtcgtgga taaccctgac atccagcaag aggtcgatat ggtgtatgca
    50341 gatcttgaac ggatgactaa aaccgtactg gaattgttct tagaaaatgg tcataaaaga
    50401 atagcctata tcgggggata taaagtcgat atggatgaat tcggtgggaa gcagatttct
    50461 ataaatgaaa agaggatgcg tgcctatcag cattttatga tcgatcatca cttagaaaaa
    50521 tatatgtctt atcatctagg tggctggaca gaaaaacaag gtgaggtact tgcgactaat
    50581 ctattagaaa atgaatcccc tcttcctact gccattcttg tcgggagcga tccaatggca
    50641 attggtgtct atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga gattgttgta
    50701 gcaagctttg ataatatcga gacaatcgca aacctctccc ctggcttgac cagtgttcaa
    50761 atcaatgcga aatcaattgg gcaagcagct gttcgaatgg cattggagcg aattgacggt
    50821 atacgggacg aaccaatcat tttgacttat cctgcacgat tgattgttag ggaaagtttt
    50881 atcccaagaa caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg tttcttcata
    50941 ctcaaaacga aacggctttt tcacaacctc ttacttatac ttattcacaa aatccaatgt
    51001 ctgctggtaa caattttgcg attcttttag cgtgtaatca aattggtatt catgggtaat
    51061 ctcttttttt gtatctgtat aaaacaagga ttgcacgggt acctttaatt ctgtcaaccg
    51121 attttctaat gcttgacctt gctcctggaa tgagtatgca tttccgtcag ttatatacgt
    51181 tggcggaaat tccttagaaa catggtcagc cacactagct tctttcaatt gctcactcgt
    51241 tttccaatct tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa acttcataaa
    51301 tcgatcatcc gaatgctgct gtgctgttag cttcaaatca acaggtccgc aataagaaat
    51361 ggttcctttc agactatctt ttggaagtag ctgtttgatt ttcatttgtt tagcatacgc
    51421 atcattcgtt tgtatagctg catattgcaa tgcgatttgt gaacctgcac tatctccacc
    51481 aaaaaataat tgttccatgt ctagcaattt gtcgtctttt tcttcagcta ataaatcttt
    51541 gatcagttca ttcacttgaa gaacttgatt agggtattct gaatcaggcg ctaattcata
    51601 attcatcgca accactgcga tgttggcgtc tgaaactaat tttgtcgcaa attctttgac
    51661 acccgattta tctccgccga cgaatcctcc accatgtacc caaaatagaa caggaacagg
    51721 ccccttgctg ttttttggat agtagatgtc ataagtattc tctttatact tggactcata
    51781 cgtcaaatct gtttttttcg ccacgttttt ttctgcttga tcgaacgttt ttttatcagt
    51841 gatttgaaca gtatttgaaa acatatggtt gatcaagaat gctccaggtc tgggggaaag
    51901 ctggaaggca agaaagattg caaacgcgat cccaagaatg attccgaaga accatccgat
    51961 aaatttaaga attttctttt tcataaggct ccctcagtag ttagtattaa gcattctgat
    52021 tataccatag tgttatttcc tatcaagctc tcctagatga tttaatgatt ttccccaaac
    52081 ttcttctgca atgcttttga caaaagaaat tttttccatt gttcttcaaa caaaacattt
    52141 tcttcttctg ttgaagcaaa tccacattga ggacttaagc acaaacgatc caaaggcaga
    52201 tatcgtgtcg cttcgtagat ccggtcaatc acttgctggt gctcttccat ttcgccattt
    52261 ttcgttacag tcaaaatcta agacaactaa tttatcttca gagactttct ctaaaagaac
    52321 aaaactccca gcacgttcac tatcgtattc taaataatag gcacggacat tttcttgagt
    52381 aaaataatgg gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg aataataatt
    52441 tccgcgtcaa acatgtatat ttagtgttga atcatttgag aatatgaaaa taaaaatctt
    52501 tttgatcaat cagttaatca aattgaaata acgaaaaatc tgagacagaa tgacgttcat
    52561 tctgacccag atttgatttt gcacaactca acagtcctta ttggcaaatc gccaaaataa
    52621 tgtagactaa c
//
LOCUS       DAJSVP010000013        51827 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSVP010000013 DAJSVP010000000
VERSION     DAJSVP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.013"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..720
                     /locus_tag="ON449_000757"
     CDS             <1..720
                     /locus_tag="ON449_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933370.1"
                     /translation="IFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF
                     FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGN
                     KRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSI
                     FLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNE
                     FMQFGLTVLGICTFIITVINR"
     gene            complement(960..1043)
                     /locus_tag="ON449_000758"
     tRNA            complement(960..1043)
                     /locus_tag="ON449_000758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1007..1009),aa:Leu,seq:gag)
     gene            1098..1277
                     /locus_tag="ON449_000759"
     CDS             1098..1277
                     /locus_tag="ON449_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933371.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1426..2643
                     /locus_tag="ON449_000760"
     CDS             1426..2643
                     /locus_tag="ON449_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3933372.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2827..3663
                     /locus_tag="ON449_000761"
     CDS             2827..3663
                     /locus_tag="ON449_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3933373.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3739..4887
                     /gene="nagA"
                     /locus_tag="ON449_000762"
     CDS             3739..4887
                     /gene="nagA"
                     /locus_tag="ON449_000762"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3933374.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5107..6165
                     /locus_tag="ON449_000763"
     CDS             5107..6165
                     /locus_tag="ON449_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3933375.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6169..7203
                     /locus_tag="ON449_000764"
     CDS             6169..7203
                     /locus_tag="ON449_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3933376.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7305..8021)
                     /locus_tag="ON449_000765"
     CDS             complement(7305..8021)
                     /locus_tag="ON449_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3933377.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7990..8775)
                     /locus_tag="ON449_000766"
     CDS             complement(7990..8775)
                     /locus_tag="ON449_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3933378.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8766..9515)
                     /locus_tag="ON449_000767"
     CDS             complement(8766..9515)
                     /locus_tag="ON449_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3933379.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9813..10337
                     /locus_tag="ON449_000768"
     CDS             9813..10337
                     /locus_tag="ON449_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3933380.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10353..11852
                     /locus_tag="ON449_000769"
     CDS             10353..11852
                     /locus_tag="ON449_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3933381.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11940..12542)
                     /locus_tag="ON449_000770"
     CDS             complement(11940..12542)
                     /locus_tag="ON449_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3933382.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12646..13602
                     /locus_tag="ON449_000771"
     CDS             12646..13602
                     /locus_tag="ON449_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3933383.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13907..14863)
                     /locus_tag="ON449_000772"
     CDS             complement(13907..14863)
                     /locus_tag="ON449_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3933384.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15037..15597
                     /locus_tag="ON449_000773"
     CDS             15037..15597
                     /locus_tag="ON449_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933385.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15590..16414
                     /locus_tag="ON449_000774"
     CDS             15590..16414
                     /locus_tag="ON449_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3933386.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16424..17041
                     /locus_tag="ON449_000775"
     CDS             16424..17041
                     /locus_tag="ON449_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3933387.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17059..17742
                     /locus_tag="ON449_000776"
     CDS             17059..17742
                     /locus_tag="ON449_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3933388.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18425..19660
                     /locus_tag="ON449_000777"
                     /pseudo
     CDS             18425..19660
                     /locus_tag="ON449_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19668..20555
                     /locus_tag="ON449_000778"
     CDS             19668..20555
                     /locus_tag="ON449_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933389.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20572..21408
                     /locus_tag="ON449_000779"
     CDS             20572..21408
                     /locus_tag="ON449_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933390.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21411..22034
                     /locus_tag="ON449_000780"
     CDS             21411..22034
                     /locus_tag="ON449_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933391.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22031..23323
                     /locus_tag="ON449_000781"
     CDS             22031..23323
                     /locus_tag="ON449_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3933392.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23682..23779
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23895..24476
                     /locus_tag="ON449_000782"
     CDS             23895..24476
                     /locus_tag="ON449_000782"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3933393.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24479..25786
                     /locus_tag="ON449_000783"
     CDS             24479..25786
                     /locus_tag="ON449_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3933394.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26079..26567
                     /gene="purE"
                     /locus_tag="ON449_000784"
     CDS             26079..26567
                     /gene="purE"
                     /locus_tag="ON449_000784"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3933395.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26560..27684
                     /gene="purK"
                     /locus_tag="ON449_000785"
     CDS             26560..27684
                     /gene="purK"
                     /locus_tag="ON449_000785"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3933396.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27697..28992
                     /gene="purB"
                     /locus_tag="ON449_000786"
     CDS             27697..28992
                     /gene="purB"
                     /locus_tag="ON449_000786"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3933397.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29172..29549)
                     /locus_tag="ON449_000787"
     CDS             complement(29172..29549)
                     /locus_tag="ON449_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933398.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29805..30533
                     /locus_tag="ON449_000788"
     CDS             29805..30533
                     /locus_tag="ON449_000788"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3933399.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30533..30787
                     /gene="purS"
                     /locus_tag="ON449_000789"
     CDS             30533..30787
                     /gene="purS"
                     /locus_tag="ON449_000789"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3933400.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30789..31460
                     /gene="purQ"
                     /locus_tag="ON449_000790"
     CDS             30789..31460
                     /gene="purQ"
                     /locus_tag="ON449_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3933401.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31461..33683
                     /gene="purL"
                     /locus_tag="ON449_000791"
     CDS             31461..33683
                     /gene="purL"
                     /locus_tag="ON449_000791"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3933402.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33668..35107
                     /locus_tag="ON449_000792"
     CDS             33668..35107
                     /locus_tag="ON449_000792"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3933403.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35130..36182
                     /locus_tag="ON449_000793"
     CDS             35130..36182
                     /locus_tag="ON449_000793"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3933404.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36179..36757
                     /gene="purN"
                     /locus_tag="ON449_000794"
     CDS             36179..36757
                     /gene="purN"
                     /locus_tag="ON449_000794"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3933405.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36754..38289
                     /gene="purH"
                     /locus_tag="ON449_000795"
     CDS             36754..38289
                     /gene="purH"
                     /locus_tag="ON449_000795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3933406.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38305..39579
                     /gene="purD"
                     /locus_tag="ON449_000796"
     CDS             38305..39579
                     /gene="purD"
                     /locus_tag="ON449_000796"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3933407.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39701..40615
                     /locus_tag="ON449_000797"
     CDS             39701..40615
                     /locus_tag="ON449_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3933408.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40748..42313)
                     /locus_tag="ON449_000798"
     CDS             complement(40748..42313)
                     /locus_tag="ON449_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3933409.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42306..43757)
                     /locus_tag="ON449_000799"
     CDS             complement(42306..43757)
                     /locus_tag="ON449_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3933410.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43771..44787)
                     /locus_tag="ON449_000800"
     CDS             complement(43771..44787)
                     /locus_tag="ON449_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3933411.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45007..46095
                     /locus_tag="ON449_000801"
     CDS             45007..46095
                     /locus_tag="ON449_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3933412.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46149..47675
                     /locus_tag="ON449_000802"
     CDS             46149..47675
                     /locus_tag="ON449_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3933413.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47677..48882
                     /locus_tag="ON449_000803"
     CDS             47677..48882
                     /locus_tag="ON449_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933414.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49139..50116
                     /gene="guaC"
                     /locus_tag="ON449_000804"
     CDS             49139..50116
                     /gene="guaC"
                     /locus_tag="ON449_000804"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3933415.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50380..51804
                     /locus_tag="ON449_000805"
     CDS             50380..51804
                     /locus_tag="ON449_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3933416.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT        16531 a         8834 c        11357 g        15105 t
ORIGIN      
        1 attttctctt tgacaactcc agggaatgca ttacgtgctg cacatgaaat taataaatgg
       61 tttcctgaat ataaaaattt caactttctt aataagttag atttaggtat ttcatcattt
      121 ggaaagccgt tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc
      181 cttctaacat ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt
      241 ttcttaaacc tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat
      301 ggaaataaaa gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta
      361 ggaacgaaac tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca
      421 ttattactat gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt
      481 tttttagtta ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc
      541 acagtatggg cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta
      601 gtactaatgg cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg
      661 caatttggat taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa
      721 tatacaaaaa atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat
      781 tgttttaaca cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc
      841 aggctgttta catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg
      901 cataaagaaa cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt
      961 gcagatgatg ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg
     1021 tctgccaatt ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat
     1081 agttgtcaag ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt
     1141 caggactggg aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg
     1201 gttttatgct aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg
     1261 actatcatga caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc
     1321 ctaaataagt ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa
     1381 aatttggaaa gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa
     1441 aaatccaaca aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg
     1501 gagcggatgc aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct
     1561 tttggatttg aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca
     1621 gaaggctact cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc
     1681 caactgaaac attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc
     1741 gaattgatca cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca
     1801 atgccggact atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat
     1861 tatatttgcg atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata
     1921 acgagtcgga caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat
     1981 ccaaaagaat tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat
     2041 tccgacgaaa tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca
     2101 ttagcgcctg atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca
     2161 ggatttcgag taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata
     2221 gaagggctga atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt
     2281 atccaaaccg cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg
     2341 atctatgaac aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc
     2401 gtaaaaccaa aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt
     2461 ttggatgatg aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat
     2521 ggtggtggat ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg
     2581 gatgatttga aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa
     2641 tgattttaaa aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct
     2701 ttctagtgga gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga
     2761 agtttgcgac ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg
     2821 atacttatgg aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa
     2881 caaaaaatga cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg
     2941 atcgaaaata atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta
     3001 ggggtaacga ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa
     3061 ttatttgaac tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta
     3121 ctaaaatctt ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat
     3181 tattatctcg gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat
     3241 ttgaaagaag cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa
     3301 atacaactga ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca
     3361 cgctactatt tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa
     3421 agtgaatttt cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc
     3481 gacgaagcgt tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac
     3541 tattacctca atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca
     3601 gaaagagcag cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg
     3661 taaatggtgt agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa
     3721 acaaagggag agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca
     3781 ggtgtcaaac atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa
     3841 gaactacctg agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt
     3901 ttggtagata cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa
     3961 ggcatcaaag tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca
     4021 acattgacgt caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat
     4081 caggaagcac caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa
     4141 gaacacaaag gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat
     4201 gaatggcaag aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa
     4261 ggtgtagaag agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac
     4321 agtaatgcta cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc
     4381 catgctttca atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta
     4441 ttatctttag aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat
     4501 gctgcgggaa tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt
     4561 atgatggccg gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta
     4621 aaagaaggaa cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa
     4681 gaagcaatta aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg
     4741 gctagtctga ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa
     4801 ggacgcgatg cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta
     4861 gatggcgtgg aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct
     4921 taagtttggt tttaaggttt ttttgtgttc tagtaaaaat cactttacat aagtttcaaa
     4981 agtttcttta atttcctatt gttcaataag taacaatgta aagataaaat taaaaatgtt
     5041 aatacgtatt agcaaaataa aagctttttt tattgtgaat aaatgaacat aattaggagg
     5101 agaaagatga acttatttga catttttcga tcgactttga cagatatgtc gattgtcagt
     5161 gcaatcactt caacagtttt tattatatta ttagggtttg tttgtagaaa aaaggggatt
     5221 ttttctgaat ctgttggaaa aattctatcg aaagtcgtct taacagtggc tttgccagca
     5281 ctggcatttc gagctttcat gcaagatatc aatacagaat tactgatgca aggaatcaat
     5341 gtcttgattt ggggattagt tatttatatt attcttattc tagtttcaaa accttttttc
     5401 ttaagctata aaggggataa gcaaacaacg ttgcgtgtcc tgacgatctt tggttctacg
     5461 acctttttcg gaacgcctat cgtcagtgcg atttatggtc ctgtcggcgt gatgtattcc
     5521 tcaattttca atattggcta ccgaatcttt ttgtattctt atggatacat aaaaatgagt
     5581 ggcttgaaga tggaagctaa aaatatcaaa gcgatgttcg tcaatccaat cattatcgct
     5641 acttttgccg gccttttcat ttggctgttc caagggtatc tgccacaagt gtcagttaca
     5701 ggtgcagaag gagagataag ccaagtagcg tttttgcgaa ttgaccagac tgcaccgtgg
     5761 ctatttcagc cgatggatta tttagctggt ttaagttcac cattggcgtg gctagcaatc
     5821 ggcgcaacat taggtgaagt aagcttcaaa aaagctgcat ctgaaaaaac ttcatggtat
     5881 tacagtctag tcaaagtggt atttgtacct gcattgaata tcgtgattct agcagttttg
     5941 acggcaacga atattctacc agtcaatttt gaagcactgg caacaatcgt tatcatgatg
     6001 gctacaccag ctgcaacagt ggcagcagcc tatgctatca gttttgatag ggaggcactt
     6061 ttagcctcca atgcatcctt actgtcaact atttttgctg tggtactgac acctgtatgg
     6121 atcgttgtat tacaaatgat tcatggcata ggtttatttt cataagaaat ggaggaagaa
     6181 agtatgagaa tcgcatgtta cggtgttcgt ccgaacgaga tagatttttt tgaaaaattg
     6241 aatatttacc attatgatct cagtctttat gaagaactgt tgacacatga caatatcgtg
     6301 tcagcaaagg accatgatgc tgtattgttg aggggaaatt gtatagcaga tgagaccaat
     6361 cttgcaaaaa tgcaggaata tgggattcgt tatgttttta cacggactgt gggagtcaat
     6421 catatcgatc tacaagcggc agctgatttt ggtatgacag tagcccgtgt accatcgtat
     6481 tcgccaaatg cgatagcaga gttatctttg acttttgcca tgatgctttt gagaaatact
     6541 gcgtatacga cgatccgaac atctttcaaa gattttcgag tggatgagca aatgtttagt
     6601 cgtgaaatca gaaattgcac agtagggatc ataggaacag gcaggatagg gttgacggaa
     6661 gctgttttgt ttaagggact aggagccaaa gtgatcggct acgatattca tcagacagaa
     6721 gctgctaaaa aagtattgac gttccactcg cttgaagagt tattagccaa aagcgatatt
     6781 gtcagtattc atgtacctca tatcccaggt gaaaacgatc aaatgatcaa tgaaggattt
     6841 ttagctcaaa tgaaaaaagg tagcatcttg attaatacag cccgtggaga gcttcaagat
     6901 aatgaagcta ttttaagagc tttgaaaagc aatcatttag ctggttttgc aaccgatgtt
     6961 tttgcaaatg aaacagaggt ttttttccga tccttcaagc catgggagac gattccagac
     7021 cctgctatcc agcaattagt agaaatgtac cctagagttc ttgtaacccc gcatgtaggt
     7081 tcaaatacag atgaggcatt atcaaatatg atttcgacaa gtttcgagaa ctttcgtgaa
     7141 ataatagaaa ctggaaaaac gaaaaatgag gtatctcttc ctaaagcccg tcaattaaga
     7201 taagaaaaca tggatattcg tcaataaaat gtttaaaaat agtccaagta aacagttgtt
     7261 acttctgtat acttggacta ttttgtatgg gataattcga tgactcaaca gtaatctgtg
     7321 agctttttcc gcccattctt tgtcaattca taacaggtta gcggctggcc gatttccaaa
     7381 tatttgattc tttcgttcaa tatatcttgt tctactaaaa aattcaaata tttcttgacg
     7441 gatatacgtg acagtgagct tttttgagcc agttcttttg ttgaaaaaat agtatcttct
     7501 tttagaataa atgcacttat tttttttaaa gtcagttttg acagaccttt aggcaaatcg
     7561 atgaatgatg gtatttcgtg aatgggagtg gtgtggttga atacttgatc gatcaatttc
     7621 tgatcggctt tttcagtcgt ttggattgtc tgagagaaag caagatactt ttcgatcgcc
     7681 agctgaaaac gttgcaaagt gaacggtttg atcaaataat ctaaggcacc gtagcggatc
     7741 acattcttta tggtttcgat atcatctgca gcagtgatca aaatcactgg gatatcattt
     7801 ccttgctgtc taagccaaga taaaaaatgg ataccacttt cgccaggcat gtatacatca
     7861 agcagcagca gatctgcgtt tttttgtctg aaatattttt ttccttcttc tacatttctg
     7921 aaactttcga ttacgttcat attagggatg ctttccacaa aagtacgatt caattctgca
     7981 accattggat catcttcgat tatcaggaca tttgtcatca ttttgatctt ccttttttgg
     8041 taattctaga tagacgattg taccgccatt tgtttgtttg gaaaattcta aaaatccacc
     8101 gtggcgtttg acgattgaat ggatgagatt caatccaaac ccacgattga ttcccttcgt
     8161 cgaaaaacct agcgtaaata ttttttctga gatttcttct gatatcccga caccgccatc
     8221 gcagacggtc acgatgaata cttcagcttc tgtgtcatag tttaaatgaa tccggacctg
     8281 tccgtttctt tcatgttttt gtagcacggc ttcttttgca ttatcaagta agttccctaa
     8341 cacttgcaaa agtgaatgga tggagtcatc catctccatt ttagggataa aagaatcgct
     8401 ttctaacagc atttcaacag attgttcttt ggcttcgttt attttgccta ataaaaaacc
     8461 ggcaattgca ggtactttga tcagacttga gacttggcca atctcttttt tataattttg
     8521 atttagctgc tggataaaat gggtgacttc ttcatacttc ttcaaatcaa tcaaaccaga
     8581 aatgacatga agtttattca tgaattcatg ggtttgagct ctcatggagt ttatatattg
     8641 tttcgtcccc ccaagctcct ccattaattt cattacttct gatcgatttc gtaaagttgc
     8701 gactgctcct aaaaaacggt cattgctaaa aagaggtgta acgctagaaa tagtttctat
     8761 cccatttaaa tacatggatt gagtccgaac ggtcgtttga tgctcaatcg tctctttaaa
     8821 caaaacgtca tatagattat cactgatttt tttcctactt cgaaatccaa tagctgctct
     8881 gtttggtaga aaatgttttt tgcaggtgta ttgatcaaga ggatagtttt atcagaagag
     8941 atggctaaga ttccttcatc tacttcattt tcgataatcg ttttttcact taactgttct
     9001 gctatttctt gtggttcaag tccaagcagt acttttttca ttttttgggc aagaaaaacg
     9061 gcgcctatga tgcctacgct aagtccaata atcaatgaaa aaacaatcat tttttgtcca
     9121 tctttgactt gattgtcaat cgtgttgttt gttaagccaa cacaaactgc accaataatc
     9181 tcttgatcat aataaatagg gacgaaatag cggaagccga accctaacat tccttctttt
     9241 tcagagaaaa attcttgtcc tccaatcact tttttcacat cttcatgact tgagaaagga
     9301 agaccgatgt aacttgggtc tgcgtgagaa tatcgaatgc cttcagggtc catgacaacg
     9361 ataaaatcta aattcgatgc aatcatgatt tcatttgtta ttccttgaat cgcatgtgtc
     9421 gggtcaccag tctgcagctt tttttggatg agtttatcat tggcaacgat tttagcaatg
     9481 ctagagattt tatctgacac actagatcgg ttcatcgaaa gaataaaatt cctgatcaat
     9541 atggcagaaa caatcaaaga aacgaatgtg gagaaacaaa cgacaaggat gatcgtgttc
     9601 tggagcttta atggtttttt cttaaaaaaa ggaaacatag gctacctcga aagaatttat
     9661 tgtgaaaatt ttaacatgaa aacaaaaatt taacaaattg tcaacgataa acacaacctt
     9721 cctataaaaa acaccactga gtcggtaaaa gaagctatac agaattttca aacattggta
     9781 tgatagacgt agaaatggaa ggaggcgtgt atatgatagg gggaatcgca cttgagacga
     9841 tttattggta tacattgctg atttgtgcag gattggcagt cctattgatt atttttggag
     9901 atgtattcga ttttgatggc ccgcttgatc cgatgcttat tatcccttgg ttaactttca
     9961 caagcttatt tggttttcta ggtgagcgtt taacagatca atcatcttta tttttattta
    10021 ttgctagtgg aggcatatcg atagtgttgg tatttttgct taatttctat atattgatgc
    10081 cgatgaaaca tgccgaatca acgatgtcgt cttctgaaaa aactctggaa ggacaagtag
    10141 ctgttgtgat cacaccgatc cctgtacgag gtatgggaga aattcagttg aaaagtgtta
    10201 ccggttccat tagtcgtcca gcttgttttt acagccccca agaacagaca gctgagcggg
    10261 gaacgaaagt attgatcatt gagatcaagg aaagaatctg ttatgtcatg ccttatgaaa
    10321 gcatgataaa agcttaaagg agcgtgtgtt aaatggaaat accagttgtt tttatcgcga
    10381 tcgcaatcgc agctttgatt ttactgatgt tgctgatcgt ctttgtagcg aagtaccaga
    10441 cagcaaaacc ggatgaagcg ttgatcatca gtggaagtta tttaggaagc aaaaatgtgc
    10501 acattgatga aagcaataac aaaatcaaaa ttgtgcgtgg aggtggcgca ttcgtcttgc
    10561 cagtttttca acgttcgaat cggatcagtc tattatcgag taaattggac gtctccaccc
    10621 ctgaagtgta tacggaacaa ggcgtaccgg tgatgtgtga tggtacttcg atcatcaaaa
    10681 tcggttcatc tgtagaagag attgctacag cagcagaaca gtttttaggc aagacaagag
    10741 aagaactgga aaatgaagca agagaagtat tagaaggaca cttgcgctcg attcttggtt
    10801 ctatgactgt agaagaaatc tatcaaaatc gcgataagtt cagtcaaagc gtacaagaag
    10861 tcgcttctgt agaccttgca aaaatggggt tgatcatcgt ttctttcact atcaaagagg
    10921 tcagagacaa aaacggttat cttgactctt tagggaaacc aagaatcgcc caagtcaaaa
    10981 gagatgcaga tatagcagaa gcggaagctc taaaagaaac gcgtatcaaa aaagctcaag
    11041 cagaaaaaga atcacaagca gctgaattac agcgacaaac agagatcgca gaatcattaa
    11101 aagaaaaaga actgaaatta gcaacctaca aacaagaaca agatgttgct aaagcaaaag
    11161 cagaccaagc ctacaattta gaaagtgccc gagcacaaca acaagtaatc gaacaggaaa
    11221 tgcagattaa aatcgtagaa cgccaaaaac aaatcgaact ggaagaaaaa gaaatcacac
    11281 gtcgtgaaaa gcaatatgat tcggaagtga agaaaaaagc agatgccgat cggtacgcta
    11341 aagaacaaga agcacaagct caaaaagtca aagaagtgac tgaagccgaa gcagaacgat
    11401 tccgtgtgga agcactggca gaagcggaag ccaataagac acgtttagct ggacaagcag
    11461 aagccgaagc agcactagca aaaggaaaag cagaagccga agcaaaacaa aagatcgcca
    11521 atgccttcaa ggaatatggc gaagcagctg tcttgagtat ggttatcgat atgctaccgc
    11581 aattgatgcg cgaagcagca cagccgctag gaaatatcga aaaaatctct gttgtcgata
    11641 caggaagcag ttcaggagaa accagtggag cgaaccgagt gacaaattat gccacaaatt
    11701 tgctttcttc tactcaagaa acactaaaag cgacaacagg acttgacttg aaggaaatcg
    11761 tagagaattt gtcgacacgt gggactacta gccaagtaaa tatgtatgaa gcgccaaaag
    11821 aggcagaaca aaactcgatg aaggaagaat aaagtagtac acttcaccgt tcaatcaaaa
    11881 atggaggaag actttttaaa gttcttctct ccattttttt attttattgc aaaagaaaat
    11941 tagtcctcgc tatttttctt ttgtttttgg atcttagctt ctatctcttg taaatttgtt
    12001 cttagcaatc gttctttctt ttggagttcg accaaatctt ctgatagttc ttccttcttt
    12061 tctgtttcag gaggacgaag aaagaaattg ataccgattg caatgaatgt ccctataaaa
    12121 gtatctagta cacgttggat agcatacaaa gcggattcgc cttgagggat gctcaacgca
    12181 atcagcaaca acgtagctat tgcggaaatg ataccggaat tattgttaat accatctgac
    12241 acgacgatca caataataac tagaatagga agcaagatca attctaccaa aaaatcattg
    12301 tgaaaatagc ttttgacaaa aaaataaatg atagctaatg taccacctaa tgtattgccg
    12361 atgatcctag actttccaaa agaaacggtt gtagtcaagt cctgacgcaa agaaaaaaca
    12421 gcagataatg cagcaatcat cgggattcct cgttgagtca aatgaaaaaa caagatacat
    12481 aaaaaaacag ctaaggcact tttgatcgta cgcataccta gtcgaaaacg gccaaattgc
    12541 atagagattc tcctttttgg tctttttact atcataccta aacttttgtc tgatagctag
    12601 tttttcatta ttcaagtata attaaaataa aaaaggggga agtaaatgcg tataggaatg
    12661 cgaacagtca aaacagtgat cagtgtcttc ttttcgatgg tcacagcggg tagcttatcg
    12721 ctgttgtatt ggccagctgc agggatcatt gcattattaa gtgtaggaaa taccaagcgc
    12781 tctacattgc gtacgggcat gaatcgaatc gcagccttta tattagctac ggcaacagca
    12841 ctgttcagtt tttttgctgt tgggtatact atttttggct tcagtctttt tttattcctg
    12901 ttcatcccgc ttgcctctcg cttcaaatta accgaaggga tagtagtaaa ttcggttttg
    12961 gtcactcatt atttagtgga ggaaaacatg tcttggcagc tgatcggcaa tgaagcggga
    13021 ttaatggcga ttggattagt atttgctttg cttgcaaatg tttacatgcc agatcgtacg
    13081 aagcagctga aggaaaacca aatacagatc gaaaaagaat ttcgaaatat tttgagacaa
    13141 atggcagaat ttcttttatc agaaaataaa gaagatgtgc agatcaaatg cgaacattta
    13201 ctgacattca ttcgcgaaag ccaagaagat gccagagaac atcaagaaaa ctactggctc
    13261 agacagccgc tatactacga aacttacttc tctatgagaa gagcacaggc caatgtgatt
    13321 aaagacatgc tggaaaatct agagagaatc cagcaaccag cttattatgg aaaacatatc
    13381 tatggtttat tgatttatac tgcagaaaca ttttcagagt caaatgatgg cagacaaatt
    13441 ttaacacgta ttgaagaagt ttatgtattg tatcggcaaa tgcctctgcc gactagtcgc
    13501 cctgaattcg aggatcgagc agaattgttc cagtttttgc agtcttttaa aagttttatt
    13561 gaaatcaagg cagaattctc ccaacaaaaa atccaaaaat agaggttgtg aattcagcta
    13621 atattcaggt caaaaagctg atttcacaac ctcttcttta aacggtgttc tcctgtcaac
    13681 tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgctcc
    13741 ttgtgactcg gagctgaacg gtaggctcac aacctctttc ttaaacggtg ttcaaaagct
    13801 ttcctctgcg gtaataagtc cagccaaacg aaaaatagga ggagctattt ttgtttggct
    13861 gttcttatta cttgatgcag aacagctttt tcacaacctt ttaaaattac atatttttcc
    13921 atagaacttg catttgatcc aagatttcga tgacatcttt tgtcaagaag agagagcgat
    13981 tgggcaagtc accttcaatc atgtgtacca tattctttat ttcgtaattc attgcttcag
    14041 atgtattccc gctttcgatg aattcctttg taccatctgt aaataagatc tccgcacgat
    14101 cagcacgcgg gtaatcagca atcgtcacat aaccgttctc aaaggcaacg ataccgactt
    14161 ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag ttcattttct ttgttacgca
    14221 agatagtgac ggattgttcg tccacgcctg tttcaaaagg aaccattgta gaggcaacta
    14281 cttcaggttg agagctcaaa aagaaacgag cgaaagatac agcatacgta ccgatatcca
    14341 acagagctcc tccagctaat tctggattga agaaccggtt tttagggtct ggttctttgt
    14401 aactgccgaa aggtgcttgg atcattttta aagcccccag ttttcctgta tccattattg
    14461 agcgtaactg ctgataaagt ggcatgttga agattgtcat cgcttctgct aagatcaaat
    14521 tcttttcttc tgctaagcgc atagcgtcag ccagttcttt tttattcatc gtgatggctt
    14581 tttcacatag aacgtgtttg cctgcttcca atgctgccaa gatatggtcg atatgctgtc
    14641 tatttggtac agcaatgtac acgatgtcga tttcagcatc gtctaacatt tcttgatagc
    14701 ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt ttccgctttt tctgaagttc
    14761 tggaagcaac tgctgttagt tcgctcgtct cttgattgaa gctttcggca aactcatgtg
    14821 cgatggtacc aagaccaacg attccccatt gatatttttt catctgaaaa cgctccttca
    14881 tagttggatt gagtttcttg tctgtctatt attataacaa aaatcacgaa gaataaagag
    14941 gagaggactt gaaacattaa aaaaaatgaa agcagcgatt taagaggtgc tttttgatca
    15001 aaacttaagt agaatagtta aaaggagggg ataaacgtgc atcaagtata tattgatctt
    15061 attatgagtg tattattaga acagtttgaa actgaaaaaa attttttaga tgaatacttg
    15121 ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg agaaaaatct gccatctgaa
    15181 gcgatgcaga aagtaaaaaa tcttttcact gattatgaat ggatgctaac gcaaaagatt
    15241 ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg ccgttgcaga gtacaagcaa
    15301 atcaaaacac gcatcgcgca aaaatggctt agcagcaaag cagcaaaagt cgagctgatc
    15361 cctttgaaaa ataatcagca ggctggtaga taccttgact tgaaagtaag tattcaatat
    15421 gatgaatggg gctatgatga tattttgaat tttcgtctgc cagcagatgt tcaaaaacag
    15481 atcgaaggag aaaaaatcga acttctcgat tgggtgaatg aaaatctgga agaaacttac
    15541 gttcatgagg cgaatgggga acgtgaagaa caacgaaagg atttttcaag tgaataaatt
    15601 tttaggattt ggagcagttg tgattatgat cgcagcaatg ggctatgtta tttttagcga
    15661 tgatgattcg catgttcaag ccggcaatca agcagtcgaa cgaacaagca ccaaaacaac
    15721 tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag gtaaaggaca aagaaacaaa
    15781 agatcgtacg tccaaacaat tgccagatgt taaaacagac gattggaagc tagttttggt
    15841 tggtccagat cacaaattag aaaaagaaat tgatgagtct actcaattgg catcattaag
    15901 taacggatac ttgatcgaca aacgcattgc tccagaatat gaagcatttg ccaaggctgc
    15961 tgaagcagct ggatttccat tagtgatggt ctcagcttac cggtctgtat catcccaaca
    16021 acaagttttc gaacaaaatg tccaagatgt gatgagtcgt caaggtgtat cagaagagga
    16081 ggcgacaaag attactaaag aaacgattac ggagccagga tacagcgaac accacacagg
    16141 tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt tatccttcta ctgtattgga
    16201 tgcaagttac ggagatcagc ctggagctaa atggatcgca gataatgctg cgcagtatgg
    16261 atttatcgtt cgttatccaa aaggacacga agatataact aagatcacat atgaaccttg
    16321 gcacttacgt tatgtcggta aagaaaacgc tgagtatatc accaaacacc aattgacgct
    16381 agaagaatat ctgaatgaat taaatgaaaa gtaggaattg atcatggcga aaaaaaagtg
    16441 gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg gttgccggtg tagcagtgat
    16501 actcttagcc tttgtttttt ccttgaagaa tctgtcttct ccttttactg ataatcagca
    16561 agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg cttgttccgc atgcccaaga
    16621 actacaagat ggttatggag tgcttcctag tatcatttta ggacaagcta tattagaatc
    16681 aaattgggga aaaagtacct tggctagcaa atacaataat cttttcggga tcaaggctta
    16741 tggggatcag aagaaagtaa gcctggaaac aaaggaattt gttaacgaag aatggataac
    16801 tatccaagga gacttcaaag tgtatgattc atgggaacag tccatggatg accatacaca
    16861 actgttcgtc caaggagtag attggaatcc tgcgctgtat gaaaaagtga ttacggccac
    16921 caactatcag gaagccgcac aagcattgca agatgcaggt tatgcgacag accccggtta
    16981 tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg aatcagtatg accggagata
    17041 gcaaaggaga agtggctgat ggcagaacaa tttacgccag aaatcatgac ggaattgaga
    17101 aaagatatcg taagagtacc ggaaatcatt ttagaagcaa gcgggatcaa gatatttgga
    17161 aaattgatcc gttcgatcat ctttactacg gatattgcga tcatacgtaa tacaaatgcg
    17221 aatgcagtaa ttgctgtgta tccttttaca cctcatccag cgattactaa aagcattatt
    17281 gaggcagctg acattccagt tttctccggt gtaggtggtg gtttgacaca aggtgctcgt
    17341 tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt taggggtcgt tttgaacggt
    17401 cctacgcctt tagagacagt aaaagaagtt tgtcgagtag ttgatattcc agttatcagt
    17461 acggtaacgt cgaaatatac agaaatagat gaaaagttag cagctggtat ttcgattatc
    17521 aatatcagtg caggtaaaga aactgcagaa gcagtacgcc attttcgtaa aaaatatcct
    17581 gaacttccta tcattgcgac aggtggtcca acggaagaaa gtattgctga gaccattcaa
    17641 gcaggagcaa atgccatcac atatacgcca ccaagtagcg gagagctttt tagtcgtaaa
    17701 atggaaaaat atcgggaaac tgagaaaaag aatcatgaat aacaggtgag aatcaaaaga
    17761 cgaggaagag cagatagtgg ctcttcctcg tcttttgtac tccagtacta tttgttcaga
    17821 attcaaaaaa aagagaaatt tttttgtttt tttacctgta atgtacataa gaagagttaa
    17881 aaccttttgt tattattagc attctaacaa aaaaatgtct tgatttattg aagcgctttc
    17941 tatagaatga aaaacgaata aaaactaaaa aaggcacccg acaatttagt agcagctaaa
    18001 ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa gttgccatag tcaatgcttg
    18061 tgcatcgacg tgtatattgt acctaatttc atacagaatt actagttatt tttctgtttt
    18121 ttagggaaga gcgcacacga agaagcatgc ttatttagct atgtcaagga tattgggcag
    18181 ttgtctgata tccttttttc tttgcgtaat tatcctccca tcattgaagc aagaagtgtt
    18241 tttagcgatg aaagaagaat cagcactatc taatttttct tgaaagtatt gagttgttca
    18301 tcctaggcag aacgataagg caagtcttgg tttttattcc ccttgaaaaa gatcgtttaa
    18361 taaatatgtt ctgtataaaa atacagtata ttgaaaaata acaatggaag tagaggtgca
    18421 gagaatgaag aaaagaatga gaatcatctt agctttaagt gttatgttat tcttcttgac
    18481 ggcttgtggg caagaagatg acaaaaaaat cacgctgaac atggcttggt ggggttcgca
    18541 agtccgacac gatgcaacag taaaagtaat cgagctttat gaagaaaaaa atccccatgt
    18601 gacgatcgat tatgaatttt atgattatga aggatattac acaaaattag gtacactagc
    18661 agcatcagat gatatctggg acattttcca actgaacaat caatttcctc aatacatcaa
    18721 tcagattgaa ccattagatg aatatgtgga gcaagggata attgatattt ctaatgcaga
    18781 tgaatcctat ttagcaacga cgacttacga cgggcaatta gttggactgt ctaacggggt
    18841 gaattcgttt gcgattgctt acaataaaaa tatatttgaa caattaaata tccctgaacc
    18901 agcttcaaat tggacatggg atgaatttga acaaatcagc tatcaaatca ctgaagaatt
    18961 aggaatctat gctatttcaa gattcgaaga ttttatagct ggctgtatta ttcaaatacc
    19021 acaagttgag aaaggattga atttttataa tcgggaagat ctatctaaca gcttgggatt
    19081 tgaaaatcca gactatctaa cagatttttt ccagctgcgt aaaaatctgg tagacaaagg
    19141 ggcccatcca gaaccaggtg gtggagctgc aagtacgacg gatctgtctt ttcaggcggt
    19201 tagggataga gaagcggcaa tggtgtttct atcaagtaac cagttgacag aaatattgtc
    19261 aggggctcct gaagatatgg agattcagtt gattaatcca cctcgcagaa aagcagatgg
    19321 agaatctggt atccctttac gttcttcgca aatgctgagt atggcaaaaa gctcagccca
    19381 taaagaagaa gctgctaagt ttattgattt ctttcaaaac agtgaagaag ccaatgaaat
    19441 tttaaaagga gaacgtggtg taccaatcat gtcgaatatt cgagaacagc tcgctcgtgc
    19501 agatgatgca actttgcaag cgacatttga ttatttagaa atgatttccg atatggacaa
    19561 cggcgaagtg aatgtctttg atagtgcttt gaatccggaa attcaagacc aatacaatct
    19621 tttagttgaa aaagtcattt atgaagaagt aacagcagaa ggaaggcatg aaaaaagaaa
    19681 aactaaaacg gttcttggat caagacaatg tggtcggtta cgtatttgct tctccattca
    19741 tcctaggatt tctattgata acagtgatac cgatgatctt gtcactttat tattcgttca
    19801 ccgattatcg tttgggaaat ccagtctcat ggatcggctt ggataattat ataaagatgc
    19861 tgagtgatgc ccgttttttg aactccatcc gagtcacttt tctctatgta tttgcatctg
    19921 tacctgtgaa actgatcttt ggtctattga ttgcttatct tttaacgagg aatatcaaag
    19981 gaacgacttt ctttcgttct ttgtattatg tgcctagttt gatcggtggc agtatttcta
    20041 tcgctctagt ttggcgggag ttgttttcta aaaacgggtt agtgaatatt ttcctggaac
    20101 gattaggcgc acctgcgctt tcctggttta gcgatcaaaa actggtcatg atccctcttg
    20161 ttttgatgtc tgcttggcaa ttcggttcgg caatgatcat ctttgctgca ggtatcaaag
    20221 agatcccagg ctcgtattat gaagcggcag agatcgatgg agcgaacaaa tggcagacgt
    20281 tttacaagat cacgttacct cttctttcgc cgatcatttt gtataacttg gttatgcaga
    20341 caatcgcggc gtttatgact tttacgcagg catttatcat tacccaaggt ggtccgaatg
    20401 atgccacgaa cttttatgct ctttatgtgt ataaccaagc tttttctttt aattctatgg
    20461 ggtatgctag tgctatttcg tggatcatgc tgttgattat gggagctgtc acctttctga
    20521 ttttcaaatc atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga aatgaataat
    20581 acagtaacga aatggtttca atacggtttt attttgctat taggtctagt catgatttat
    20641 ccggtcttat ggatgatcgc aggttctttt aaaacggatc aagagattat gagtggttcg
    20701 ctgaatttga ttccagagac ttttcaatgg gaaaattacg caaaaggatg ggcggggttt
    20761 gctggaattt cattttttac tttctttagg aattcgtttg tcatctctgc agtttcgacg
    20821 atagggacag ttctgagctc gacttgtatt gcttacgcat tatcgagaat cgatttcaaa
    20881 ggaaaaagat tctggttcgt cgtcatgtta gtaacgatga tgattcctgc acaagtgatt
    20941 ttgattccgc agtttattat ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg
    21001 attttaccac atttttttgg acaagcattt ttcatttacc agatcatgca attcatggtc
    21061 agcatcccca aagagttaga tgaatcagcg attattgacg gatgcagcaa gtatagtgtc
    21121 tttaccaaaa ttatttttcc tttgctgaaa ccttcgatca tcacgacgat catcatccag
    21181 ttttattgga aatgggacga cttcatggga ccattgattt atttaaacaa gccgagatct
    21241 tacacggtat cgattgccat caaattattt gcagacgctg gatctagtac gacttacgcg
    21301 agtatgtttg cgatgtctac gttgtcgctg ctaccggttt ttctgatttt cttattcttc
    21361 aataaatatc ttgtccaggg gatcagtaca agcggtctga agggctgaat atgaaaaagc
    21421 caatagaaga gatgtttctt tacaagctca actactatgt ctgtcggatt tttttaacca
    21481 gtcttatcag taatgctctt ttcgctttag ttctaggatg tttgttcact ttaggattag
    21541 tgctgtctgc tcgtgttgtc tatctgattt attttttgat gatgtatcct tcttattatc
    21601 tatctgtcca ttacttggca gaaaaaaagg tatgttggag gaaagggaaa atcaaacgga
    21661 tgtgtttcat cattacgttg tttcagctgt ttgtcattat ttttgcagtt gattggtatt
    21721 tattagtggg aaagattgga gcttggtatc tgattccttt tctattgtgt ctgttgcttt
    21781 ttgtatccca tagttttttt agtacgctaa ttgatatctg caaagaagat aaagcagata
    21841 gtcttgttta tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc
    21901 tgtggtattt atgccttttg tatggactgt tctggtgtat acagcctgat tttttactca
    21961 ctgcattact gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga
    22021 atgactttaa atgaagaaaa aatatgtaca agtcggactt ggtgggagag cgcgtttttt
    22081 ttatgaggca attgcctcta cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt
    22141 atctcaaaca aggatggatt atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa
    22201 agtaaagaca tgtatgccat cacaatttga gcaactactg gatgacgtac ggccggctga
    22261 agtcattatt acgtcagtag atcgaacgca tcatgattat atcattcgtg caatgaaaaa
    22321 aggatatgat gttatttgcg aaaaacctct gactatcgat gaggaaaaag ctcaagaaat
    22381 aataacggtc caaaaacaga cgggaagaag cctgcgagtc acttttaatt atcgttatgc
    22441 accgtatcac acgaagataa aggaactgat agaaagtgat gtgattggtg atgtgttttc
    22501 tatccacttc gaatggctgt taaatacgca acacggggca gattatttca gaagatggca
    22561 tcgaaataag cacaatagcg gcgggcttct tgttcataaa tctacccatc attttgatct
    22621 agtgaatttt tggttgaaca gccgtcccca aaaggtattt gcattcggtg gattaaactt
    22681 ttatgggatg cataatgcgg agaaacgcgg agtagaaata ttttacagtc gatgctatgg
    22741 aagcgaacta gctaaagctg atccatttgc tatagacctg tcagccaatg aggaattgcg
    22801 agaaatgtat ttagaagcag aaaaagacag tggatatatt cgtgaccgca gtgtattttc
    22861 cgatgatatc tctattgaag atacactagg cgtaacggtc aaatatgaaa ctggtgcgat
    22921 tttgacgtat tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg
    22981 cagcaaagga cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg caggtggaga
    23041 taaaaaggat gaaggagcag ccgttcttca gtccatccat gtctatccga tgttcggtga
    23101 gccatatgag gtaatcgtcg aacaagaagc gggcggacat ggcggaggag atccgcgttt
    23161 attagatgat ctatttggtg aagaaaaaga agaagatccg tggaatcggg cagcaactca
    23221 cgtagatggt attttgtcga ttttgacagg aatcgcagca aatcattcta ttgcttcagg
    23281 aaaagcagta gtgattgacg agcttgtcag cttaaaagaa taagaataga tttacggaaa
    23341 ttacttcccg tagtttataa aatcttgcct tattgccgat aggctgtctt ttgaacacta
    23401 tttattcgga aaaaggggct atgacatgct ttttgtcatg ccttcttttt atgtgggctg
    23461 cttttttaga taggagaaaa taaaaataac tatttaaacc cgaacaaaaa ggaataatgc
    23521 attaaaaatc attagatttt acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt
    23581 agtgttagac tgttttcaat atttgaaaca acatttttta ttcaaatatg gcaaactgcc
    23641 ttcacgtgca aaacagttgc aagaaaatga atttacacta ttattagttt tacataccta
    23701 cttatatatc gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat
    23761 aagtaaaaga ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct
    23821 ttaacagaaa catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata
    23881 aaggagcaaa taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag
    23941 gcgagggcgt attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg
    24001 aacaaatggg aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga
    24061 ccattgaagc ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa
    24121 tgatcttcgc tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg
    24181 tttattcttt tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt
    24241 cagaagacaa agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat
    24301 tgatcgaatt atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa
    24361 agtctttcca agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg
    24421 cgcgtattgc ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt
    24481 ggaaaaagaa acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat
    24541 gtatgcagga gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga
    24601 acagatgact tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca
    24661 attgacagtt aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca
    24721 agcagtagct ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc
    24781 tattatcgct tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa
    24841 actattccct cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc
    24901 tgtagcaatc gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac
    24961 aaatctgcta cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa
    25021 agggttcatt cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc
    25081 tttcatggga atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca
    25141 acccttttat tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat
    25201 ttccatggtc agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg
    25261 gaaaaatgtt ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat
    25321 cttaggcggt attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt
    25381 tcaactttcg ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt
    25441 tttaggatta ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg
    25501 tggaggaatg ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga
    25561 agttgatttc agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct
    25621 gggtttcaat gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac
    25681 tggaaatggc atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg
    25741 attgaaaaaa gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa
    25801 ggttgtgaaa tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc
    25861 gtattttgcc gtttgtttga gctaataccg agggtcagct gattgaacac cgtttatcca
    25921 taaaaaggaa tgtgacctaa cttttgtcac attccttttt ttcatcttta ataaacacga
    25981 acaataaaat gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt
    26041 gttaaaatat gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga
    26101 ttatggggag tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat
    26161 tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg
    26221 aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag
    26281 ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg
    26341 tccaatcacg aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag
    26401 gagtacctgt agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg
    26461 cgatacagat gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt
    26521 caatgttggc taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct
    26581 ggttcaacga tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca
    26641 aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa
    26701 gttgcagatt ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc
    26761 agaagaacag atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg
    26821 attttaccca tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc
    26881 ctgttagaaa aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc
    26941 gtcagtccga cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa
    27001 accactcgag gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta
    27061 gctcctgcca tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt
    27121 gaaaaagaga tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg
    27181 gtcgtggaaa acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt
    27241 gatcaggatg tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc
    27301 ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat
    27361 gaattagctc ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt
    27421 cagttcgatg cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg
    27481 ataagcaatg cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg
    27541 attgagaata agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt
    27601 cgcaagatgg gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc
    27661 aaacaaacga atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct
    27721 gaaatgggga atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt
    27781 ttagcagatg aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt
    27841 cgtgagcatg catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat
    27901 gatgtcgttg cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc
    27961 cattatggtc taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa
    28021 gcaaatgata ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca
    28081 aaagaacaca aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact
    28141 acttttggat tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt
    28201 gaacatgcgg caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca
    28261 aatatcccgc catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa
    28321 atctctactc aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt
    28381 attgctacaa gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca
    28441 cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa
    28501 aggaatccga tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg
    28561 gtcactgcct atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca
    28621 gaacgaatca tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc
    28681 aatatcgtta aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca
    28741 tttggtttga tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga
    28801 gaagaagctt atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag
    28861 ttccgcccgc tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata
    28921 gacgatgcct ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta
    28981 ggtttggaat aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac
    29041 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
    29101 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
    29161 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
    29221 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
    29281 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
    29341 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
    29401 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
    29461 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
    29521 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
    29581 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
    29641 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatacct taaatgtaca
    29701 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
    29761 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
    29821 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
    29881 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
    29941 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
    30001 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
    30061 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
    30121 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
    30181 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaaaccgg
    30241 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
    30301 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
    30361 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
    30421 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
    30481 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
    30541 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
    30601 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
    30661 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
    30721 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
    30781 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
    30841 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
    30901 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
    30961 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
    31021 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
    31081 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
    31141 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
    31201 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
    31261 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
    31321 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
    31381 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
    31441 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
    31501 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
    31561 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
    31621 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
    31681 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
    31741 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
    31801 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
    31861 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
    31921 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
    31981 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
    32041 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
    32101 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
    32161 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
    32221 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
    32281 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
    32341 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
    32401 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
    32461 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
    32521 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
    32581 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
    32641 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
    32701 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
    32761 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
    32821 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
    32881 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
    32941 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
    33001 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
    33061 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
    33121 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
    33181 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
    33241 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
    33301 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
    33361 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
    33421 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
    33481 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
    33541 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
    33601 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
    33661 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
    33721 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
    33781 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
    33841 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
    33901 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
    33961 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
    34021 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
    34081 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
    34141 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
    34201 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
    34261 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
    34321 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
    34381 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
    34441 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
    34501 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
    34561 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
    34621 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
    34681 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
    34741 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
    34801 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
    34861 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
    34921 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
    34981 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
    35041 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
    35101 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
    35161 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
    35221 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
    35281 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
    35341 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
    35401 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
    35461 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
    35521 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
    35581 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
    35641 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
    35701 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
    35761 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
    35821 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
    35881 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
    35941 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
    36001 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg
    36061 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
    36121 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
    36181 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
    36241 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
    36301 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
    36361 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
    36421 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
    36481 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
    36541 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
    36601 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
    36661 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
    36721 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
    36781 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
    36841 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
    36901 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
    36961 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
    37021 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
    37081 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
    37141 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
    37201 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
    37261 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
    37321 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
    37381 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
    37441 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
    37501 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
    37561 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
    37621 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
    37681 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
    37741 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
    37801 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
    37861 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
    37921 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
    37981 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
    38041 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
    38101 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
    38161 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
    38221 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
    38281 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
    38341 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
    38401 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
    38461 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
    38521 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
    38581 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
    38641 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
    38701 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
    38761 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
    38821 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
    38881 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
    38941 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
    39001 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    39061 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    39121 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    39181 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    39241 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    39301 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    39361 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    39421 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    39481 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    39541 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    39601 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    39661 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    39721 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    39781 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    39841 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    39901 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    39961 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    40021 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    40081 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    40141 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    40201 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    40261 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    40321 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    40381 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    40441 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    40501 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    40561 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    40621 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    40681 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    40741 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    40801 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    40861 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    40921 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    40981 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    41041 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    41101 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    41161 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    41221 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    41281 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    41341 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    41401 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    41461 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    41521 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    41581 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    41641 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    41701 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    41761 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    41821 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    41881 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    41941 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    42001 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    42061 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    42121 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    42181 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    42241 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    42301 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    42361 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    42421 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    42481 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    42541 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgcggtaat acgaataaat
    42601 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    42661 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    42721 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    42781 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    42841 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    42901 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    42961 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    43021 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    43081 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    43141 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    43201 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    43261 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    43321 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    43381 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    43441 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    43501 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    43561 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    43621 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    43681 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    43741 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    43801 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    43861 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    43921 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    43981 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    44041 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    44101 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    44161 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    44221 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    44281 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    44341 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    44401 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    44461 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    44521 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    44581 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    44641 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    44701 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    44761 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    44821 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    44881 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    44941 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    45001 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    45061 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    45121 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    45181 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    45241 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    45301 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    45361 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    45421 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    45481 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    45541 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    45601 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    45661 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    45721 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    45781 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    45841 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    45901 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    45961 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    46021 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    46081 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    46141 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    46201 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    46261 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    46321 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    46381 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    46441 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    46501 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    46561 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    46621 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    46681 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    46741 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    46801 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    46861 ggatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    46921 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    46981 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    47041 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    47101 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    47161 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    47221 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    47281 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    47341 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    47401 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    47461 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    47521 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    47581 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    47641 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    47701 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    47761 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    47821 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    47881 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    47941 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    48001 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    48061 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    48121 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    48181 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    48241 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    48301 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    48361 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    48421 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    48481 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    48541 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    48601 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    48661 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    48721 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    48781 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    48841 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    48901 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    48961 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    49021 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    49081 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    49141 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    49201 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    49261 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    49321 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    49381 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    49441 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    49501 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    49561 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    49621 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    49681 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    49741 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    49801 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    49861 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    49921 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    49981 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    50041 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    50101 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    50161 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    50221 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    50281 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    50341 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    50401 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    50461 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    50521 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    50581 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    50641 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    50701 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    50761 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    50821 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    50881 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    50941 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    51001 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    51061 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    51121 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    51181 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    51241 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    51301 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    51361 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    51421 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    51481 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    51541 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    51601 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    51661 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    51721 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    51781 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaa
//
LOCUS       DAJSVP010000014        51006 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSVP010000014 DAJSVP010000000
VERSION     DAJSVP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.014"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            110..955
                     /locus_tag="ON449_000806"
     CDS             110..955
                     /locus_tag="ON449_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3933417.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1236..2456
                     /locus_tag="ON449_000807"
     CDS             1236..2456
                     /locus_tag="ON449_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3933418.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2453..5173
                     /locus_tag="ON449_000808"
     CDS             2453..5173
                     /locus_tag="ON449_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933419.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5157..6101
                     /locus_tag="ON449_000809"
     CDS             5157..6101
                     /locus_tag="ON449_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3933420.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6170..7180)
                     /locus_tag="ON449_000810"
     CDS             complement(6170..7180)
                     /locus_tag="ON449_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3933421.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7360..7704)
                     /locus_tag="ON449_000811"
     CDS             complement(7360..7704)
                     /locus_tag="ON449_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3933422.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7697..8131)
                     /locus_tag="ON449_000812"
     CDS             complement(7697..8131)
                     /locus_tag="ON449_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3933423.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8269..9006
                     /locus_tag="ON449_000813"
     CDS             8269..9006
                     /locus_tag="ON449_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933424.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8999..10210
                     /locus_tag="ON449_000814"
     CDS             8999..10210
                     /locus_tag="ON449_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933425.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10414..11196
                     /locus_tag="ON449_000815"
     CDS             10414..11196
                     /locus_tag="ON449_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3933426.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11266..11928
                     /gene="trmB"
                     /locus_tag="ON449_000816"
     CDS             11266..11928
                     /gene="trmB"
                     /locus_tag="ON449_000816"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3933427.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12026..13780)
                     /gene="ade"
                     /locus_tag="ON449_000817"
     CDS             complement(12026..13780)
                     /gene="ade"
                     /locus_tag="ON449_000817"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3933428.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13912..14841
                     /gene="tadA"
                     /locus_tag="ON449_000818"
     CDS             13912..14841
                     /gene="tadA"
                     /locus_tag="ON449_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3933429.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14798..15847
                     /locus_tag="ON449_000819"
     CDS             14798..15847
                     /locus_tag="ON449_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR3933430.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15844..16122
                     /locus_tag="ON449_000820"
     CDS             15844..16122
                     /locus_tag="ON449_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3933431.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16097..16567
                     /locus_tag="ON449_000821"
     CDS             16097..16567
                     /locus_tag="ON449_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3933432.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16584..16886
                     /locus_tag="ON449_000822"
     CDS             16584..16886
                     /locus_tag="ON449_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933433.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16903..17274
                     /locus_tag="ON449_000823"
     CDS             16903..17274
                     /locus_tag="ON449_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3933434.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17246..17605
                     /locus_tag="ON449_000824"
     CDS             17246..17605
                     /locus_tag="ON449_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933435.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17777..18784
                     /locus_tag="ON449_000825"
     CDS             17777..18784
                     /locus_tag="ON449_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3933436.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18809..19993
                     /locus_tag="ON449_000826"
     CDS             18809..19993
                     /locus_tag="ON449_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3933437.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20430..22013)
                     /locus_tag="ON449_000827"
     CDS             complement(20430..22013)
                     /locus_tag="ON449_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3933438.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22591..22734)
                     /locus_tag="ON449_000828"
     CDS             complement(22591..22734)
                     /locus_tag="ON449_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3933439.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22957..23436)
                     /locus_tag="ON449_000829"
     CDS             complement(22957..23436)
                     /locus_tag="ON449_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3933440.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23509..23793)
                     /locus_tag="ON449_000830"
     CDS             complement(23509..23793)
                     /locus_tag="ON449_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933441.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23871..24065)
                     /gene="ssrS"
                     /locus_tag="ON449_000831"
     ncRNA           complement(23871..24065)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON449_000831"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24137..25423)
                     /locus_tag="ON449_000832"
     CDS             complement(24137..25423)
                     /locus_tag="ON449_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3933442.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25561..26232
                     /locus_tag="ON449_000833"
     CDS             25561..26232
                     /locus_tag="ON449_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3933443.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26198..26686
                     /locus_tag="ON449_000834"
     CDS             26198..26686
                     /locus_tag="ON449_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3933444.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26699..27646
                     /gene="prmA"
                     /locus_tag="ON449_000835"
     CDS             26699..27646
                     /gene="prmA"
                     /locus_tag="ON449_000835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3933445.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27647..28411
                     /locus_tag="ON449_000836"
     CDS             27647..28411
                     /locus_tag="ON449_000836"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3933446.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28644..30857
                     /locus_tag="ON449_000837"
     CDS             28644..30857
                     /locus_tag="ON449_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3933447.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30868..31314
                     /locus_tag="ON449_000838"
     CDS             30868..31314
                     /locus_tag="ON449_000838"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3933448.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31485..32744
                     /locus_tag="ON449_000839"
     CDS             31485..32744
                     /locus_tag="ON449_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3933449.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32833..33780)
                     /locus_tag="ON449_000840"
     CDS             complement(32833..33780)
                     /locus_tag="ON449_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3933450.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34174..35466
                     /locus_tag="ON449_000841"
     CDS             34174..35466
                     /locus_tag="ON449_000841"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3933451.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35481..37250
                     /gene="aspS"
                     /locus_tag="ON449_000842"
     CDS             35481..37250
                     /gene="aspS"
                     /locus_tag="ON449_000842"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3933452.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37359..38234
                     /locus_tag="ON449_000843"
     CDS             37359..38234
                     /locus_tag="ON449_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3933453.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38500..40383
                     /locus_tag="ON449_000844"
     CDS             38500..40383
                     /locus_tag="ON449_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3933454.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40458..40742)
                     /locus_tag="ON449_000845"
     CDS             complement(40458..40742)
                     /locus_tag="ON449_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3933455.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40732..41466)
                     /locus_tag="ON449_000846"
     CDS             complement(40732..41466)
                     /locus_tag="ON449_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3933456.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41595..42218
                     /locus_tag="ON449_000847"
     CDS             41595..42218
                     /locus_tag="ON449_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3933457.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42410..43531
                     /locus_tag="ON449_000848"
     CDS             42410..43531
                     /locus_tag="ON449_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3933458.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43538..46666
                     /locus_tag="ON449_000849"
     CDS             43538..46666
                     /locus_tag="ON449_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3933459.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46685..49879
                     /locus_tag="ON449_000850"
     CDS             46685..49879
                     /locus_tag="ON449_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3933460.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50025..50732)
                     /locus_tag="ON449_000851"
     CDS             complement(50025..50732)
                     /locus_tag="ON449_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3933461.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16685 a         8925 c        10609 g        14787 t
ORIGIN      
        1 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
       61 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      121 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      181 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      241 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      301 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      361 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      421 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      481 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      541 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      601 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      661 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      721 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      781 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
      841 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
      901 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
      961 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1021 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1081 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1141 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1201 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1261 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1321 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1381 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1441 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1501 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1561 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1621 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1681 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1741 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1801 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     1861 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     1921 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     1981 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2041 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2101 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2161 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2221 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2281 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2341 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2401 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2461 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2521 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2581 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2641 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2701 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2761 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     2821 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     2881 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     2941 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3001 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3061 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3121 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3181 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3241 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3301 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3361 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3421 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3481 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3541 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3601 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3661 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3721 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3781 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     3841 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     3901 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     3961 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4021 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4081 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4141 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4201 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4261 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4321 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4381 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4441 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4501 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4561 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4621 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4681 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4741 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4801 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     4861 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     4921 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     4981 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5041 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5101 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5161 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5221 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5281 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5341 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5401 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5461 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5521 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5581 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5641 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5701 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5761 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     5821 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     5881 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     5941 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6001 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6061 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6121 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6181 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6241 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6301 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6361 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6421 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6481 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6541 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6601 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6661 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6721 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6781 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     6841 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     6901 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     6961 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7021 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7081 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7141 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7201 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7261 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7321 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7381 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7441 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7501 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7561 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7621 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7681 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7741 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7801 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     7861 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     7921 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     7981 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8041 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8101 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8161 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8221 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8281 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8341 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8401 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8461 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8521 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8581 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8641 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8701 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8761 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     8821 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     8881 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     8941 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9001 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9061 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9121 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9181 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9241 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9301 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9361 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9421 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9481 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9541 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9601 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9661 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9721 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9781 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
     9841 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
     9901 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
     9961 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10021 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10081 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10141 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10201 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10261 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10321 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10381 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10441 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10501 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10561 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10621 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10681 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10741 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10801 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    10861 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    10921 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    10981 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11041 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11101 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11161 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11221 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11281 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11341 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11401 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11461 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11521 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11581 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11641 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11701 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11761 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    11821 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    11881 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    11941 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12001 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12061 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12121 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12181 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12241 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12301 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12361 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12421 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12481 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12541 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12601 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12661 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12721 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12781 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    12841 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    12901 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    12961 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13021 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13081 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13141 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13201 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13261 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13321 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13381 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13441 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13501 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13561 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13621 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13681 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13741 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13801 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    13861 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    13921 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    13981 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14041 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14101 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14161 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14221 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14281 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14341 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14401 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14461 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14521 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14581 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14641 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14701 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14761 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    14821 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    14881 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    14941 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15001 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15061 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15121 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15181 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15241 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15301 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15361 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15421 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15481 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15541 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15601 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15661 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15721 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15781 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    15841 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    15901 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    15961 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16021 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16081 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16141 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16201 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16261 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16321 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16381 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16441 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16501 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16561 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16621 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16681 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16741 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16801 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    16861 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    16921 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    16981 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17041 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17101 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17161 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17221 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17281 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17341 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17401 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17461 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17521 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17581 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17641 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17701 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17761 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    17821 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    17881 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    17941 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18001 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18061 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18121 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18181 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18241 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18301 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18361 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18421 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18481 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18541 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18601 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18661 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18721 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18781 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    18841 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    18901 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    18961 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19021 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19081 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19141 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19201 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19261 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19321 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19381 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19441 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19501 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19561 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19621 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19681 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19741 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19801 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    19861 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    19921 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    19981 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20041 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20101 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20161 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20221 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20281 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20341 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20401 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20461 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20521 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20581 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20641 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20701 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20761 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    20821 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    20881 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    20941 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21001 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21061 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21121 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21181 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21241 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21301 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21361 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21421 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21481 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21541 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21601 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21661 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21721 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21781 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    21841 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    21901 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    21961 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22021 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22081 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22141 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22201 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22261 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22321 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22381 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22441 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22501 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22561 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22621 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22681 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22741 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22801 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    22861 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    22921 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    22981 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23041 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23101 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23161 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23221 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23281 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23341 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23401 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23461 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23521 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23581 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23641 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23701 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23761 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    23821 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    23881 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    23941 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24001 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24061 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24121 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24181 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24241 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24301 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24361 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24421 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24481 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24541 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24601 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24661 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24721 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24781 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    24841 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    24901 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    24961 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25021 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25081 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25141 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25201 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25261 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25321 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25381 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25441 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25501 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25561 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25621 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25681 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25741 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25801 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    25861 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    25921 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    25981 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26041 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26101 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26161 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26221 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26281 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26341 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26401 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26461 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26521 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26581 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26641 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26701 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26761 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    26821 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    26881 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    26941 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27001 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27061 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27121 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27181 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27241 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27301 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27361 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27421 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27481 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27541 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27601 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27661 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27721 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27781 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    27841 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    27901 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    27961 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28021 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28081 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28141 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28201 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28261 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28321 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28381 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28441 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28501 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28561 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28621 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28681 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28741 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28801 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    28861 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    28921 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    28981 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29041 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29101 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29161 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29221 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29281 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29341 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29401 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29461 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29521 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29581 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29641 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29701 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29761 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    29821 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    29881 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    29941 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30001 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30061 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30121 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30181 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30241 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30301 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30361 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30421 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30481 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30541 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30601 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30661 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30721 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30781 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    30841 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    30901 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    30961 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31021 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31081 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31141 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31201 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31261 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31321 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31381 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31441 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31501 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31561 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31621 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31681 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31741 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31801 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    31861 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    31921 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    31981 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32041 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32101 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32161 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32221 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32281 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32341 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32401 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32461 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32521 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32581 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32641 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32701 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32761 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    32821 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    32881 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    32941 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33001 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33061 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33121 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33181 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33241 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33301 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33361 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33421 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33481 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33541 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33601 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33661 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33721 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33781 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    33841 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    33901 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    33961 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34021 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34081 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34141 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34201 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34261 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34321 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34381 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34441 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34501 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34561 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34621 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34681 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34741 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34801 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    34861 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    34921 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    34981 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35041 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35101 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35161 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35221 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35281 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35341 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35401 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35461 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35521 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35581 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35641 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35701 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35761 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    35821 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    35881 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    35941 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36001 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36061 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36121 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36181 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36241 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36301 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36361 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36421 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36481 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36541 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36601 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36661 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36721 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36781 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    36841 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    36901 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    36961 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37021 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37081 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37141 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37201 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37261 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37321 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37381 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37441 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37501 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37561 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37621 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37681 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37741 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37801 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    37861 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    37921 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    37981 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38041 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38101 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38161 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38221 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38281 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38341 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38401 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38461 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38521 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38581 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38641 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38701 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38761 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    38821 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    38881 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    38941 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39001 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39061 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39121 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39181 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39241 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39301 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39361 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39421 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39481 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39541 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39601 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39661 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39721 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39781 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    39841 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    39901 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    39961 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40021 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40081 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40141 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40201 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40261 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40321 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40381 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40441 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40501 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40561 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40621 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40681 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40741 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40801 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    40861 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    40921 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    40981 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41041 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41101 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41161 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41221 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41281 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41341 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41401 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41461 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41521 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41581 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41641 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41701 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41761 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    41821 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    41881 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    41941 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42001 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42061 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42121 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42181 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42241 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42301 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42361 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42421 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42481 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42541 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42601 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42661 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42721 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42781 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    42841 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    42901 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    42961 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43021 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43081 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43141 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43201 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43261 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43321 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43381 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43441 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43501 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43561 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43621 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43681 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43741 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43801 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    43861 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    43921 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    43981 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44041 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44101 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44161 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44221 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44281 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44341 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44401 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44461 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44521 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44581 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44641 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44701 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44761 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    44821 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    44881 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    44941 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45001 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45061 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45121 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45181 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45241 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45301 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45361 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45421 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45481 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45541 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45601 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45661 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45721 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45781 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    45841 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    45901 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    45961 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46021 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46081 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46141 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46201 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46261 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46321 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46381 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46441 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46501 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46561 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46621 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46681 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46741 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46801 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    46861 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    46921 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    46981 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47041 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47101 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47161 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47221 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47281 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47341 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47401 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47461 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47521 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47581 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47641 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47701 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47761 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    47821 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    47881 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    47941 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48001 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48061 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48121 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48181 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48241 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48301 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48361 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48421 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48481 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48541 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48601 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48661 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48721 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48781 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    48841 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    48901 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    48961 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49021 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49081 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49141 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49201 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49261 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49321 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49381 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49441 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49501 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49561 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49621 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49681 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49741 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49801 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    49861 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    49921 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    49981 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50041 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50101 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50161 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50221 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50281 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50341 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50401 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50461 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50521 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50581 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50641 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50701 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50761 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    50821 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    50881 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    50941 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51001 ttaata
//
LOCUS       DAJSVP010000015        48500 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSVP010000015 DAJSVP010000000
VERSION     DAJSVP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.015"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..1576
                     /locus_tag="ON449_000852"
     CDS             17..1576
                     /locus_tag="ON449_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3933462.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(1687..3618)
                     /locus_tag="ON449_000853"
     CDS             complement(1687..3618)
                     /locus_tag="ON449_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3933463.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3636..4550)
                     /gene="pfkB"
                     /locus_tag="ON449_000854"
     CDS             complement(3636..4550)
                     /gene="pfkB"
                     /locus_tag="ON449_000854"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3933464.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(4547..5299)
                     /locus_tag="ON449_000855"
     CDS             complement(4547..5299)
                     /locus_tag="ON449_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3933465.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(5458..5667)
                     /locus_tag="ON449_000856"
     CDS             complement(5458..5667)
                     /locus_tag="ON449_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933466.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(5678..7006)
                     /locus_tag="ON449_000857"
     CDS             complement(5678..7006)
                     /locus_tag="ON449_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933467.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            7142..7297
                     /locus_tag="ON449_000858"
     CDS             7142..7297
                     /locus_tag="ON449_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933468.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(7371..7958)
                     /locus_tag="ON449_000859"
     CDS             complement(7371..7958)
                     /locus_tag="ON449_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3933469.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8041..9291)
                     /gene="clpX"
                     /locus_tag="ON449_000860"
     CDS             complement(8041..9291)
                     /gene="clpX"
                     /locus_tag="ON449_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3933470.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(9538..10827)
                     /locus_tag="ON449_000861"
     CDS             complement(9538..10827)
                     /locus_tag="ON449_000861"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3933471.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(10981..11913)
                     /locus_tag="ON449_000862"
     CDS             complement(10981..11913)
                     /locus_tag="ON449_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933472.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(12070..12729)
                     /locus_tag="ON449_000863"
     CDS             complement(12070..12729)
                     /locus_tag="ON449_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3933473.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(12732..13757)
                     /gene="sppA"
                     /locus_tag="ON449_000864"
     CDS             complement(12732..13757)
                     /gene="sppA"
                     /locus_tag="ON449_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3933474.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(13770..14060)
                     /locus_tag="ON449_000865"
     CDS             complement(13770..14060)
                     /locus_tag="ON449_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933475.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14470..16275
                     /gene="glmS"
                     /locus_tag="ON449_000866"
     CDS             14470..16275
                     /gene="glmS"
                     /locus_tag="ON449_000866"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3933476.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(16347..17702)
                     /locus_tag="ON449_000867"
     CDS             complement(16347..17702)
                     /locus_tag="ON449_000867"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3933477.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(17726..18895)
                     /locus_tag="ON449_000868"
     CDS             complement(17726..18895)
                     /locus_tag="ON449_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933478.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(18892..19779)
                     /locus_tag="ON449_000869"
     CDS             complement(18892..19779)
                     /locus_tag="ON449_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3933479.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(19968..23648)
                     /gene="nifJ"
                     /locus_tag="ON449_000870"
     CDS             complement(19968..23648)
                     /gene="nifJ"
                     /locus_tag="ON449_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3933480.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            23846..24367
                     /locus_tag="ON449_000871"
     CDS             23846..24367
                     /locus_tag="ON449_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3933481.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(25066..25631)
                     /locus_tag="ON449_000872"
                     /pseudo
     CDS             complement(25066..25631)
                     /locus_tag="ON449_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25810..26466)
                     /locus_tag="ON449_000873"
     CDS             complement(25810..26466)
                     /locus_tag="ON449_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933482.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(27034..27969)
                     /gene="whiA"
                     /locus_tag="ON449_000874"
     CDS             complement(27034..27969)
                     /gene="whiA"
                     /locus_tag="ON449_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3933483.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(28003..29001)
                     /locus_tag="ON449_000875"
     CDS             complement(28003..29001)
                     /locus_tag="ON449_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3933484.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(28998..29882)
                     /gene="rapZ"
                     /locus_tag="ON449_000876"
     CDS             complement(28998..29882)
                     /gene="rapZ"
                     /locus_tag="ON449_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3933485.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(30017..30748)
                     /locus_tag="ON449_000877"
     CDS             complement(30017..30748)
                     /locus_tag="ON449_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3933486.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(30752..32017)
                     /locus_tag="ON449_000878"
     CDS             complement(30752..32017)
                     /locus_tag="ON449_000878"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3933487.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(32280..35096)
                     /gene="uvrA"
                     /locus_tag="ON449_000879"
     CDS             complement(32280..35096)
                     /gene="uvrA"
                     /locus_tag="ON449_000879"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3933488.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(35106..37100)
                     /gene="uvrB"
                     /locus_tag="ON449_000880"
     CDS             complement(35106..37100)
                     /gene="uvrB"
                     /locus_tag="ON449_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3933489.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            37359..38861
                     /locus_tag="ON449_000881"
     CDS             37359..38861
                     /locus_tag="ON449_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3933490.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            38863..39600
                     /locus_tag="ON449_000882"
     CDS             38863..39600
                     /locus_tag="ON449_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3933491.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(39968..41134)
                     /gene="dnaJ"
                     /locus_tag="ON449_000883"
     CDS             complement(39968..41134)
                     /gene="dnaJ"
                     /locus_tag="ON449_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3933492.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(41285..43114)
                     /gene="dnaK"
                     /locus_tag="ON449_000884"
     CDS             complement(41285..43114)
                     /gene="dnaK"
                     /locus_tag="ON449_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3933493.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(43165..43728)
                     /gene="grpE"
                     /locus_tag="ON449_000885"
     CDS             complement(43165..43728)
                     /gene="grpE"
                     /locus_tag="ON449_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3933494.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(43822..44865)
                     /gene="hrcA"
                     /locus_tag="ON449_000886"
     CDS             complement(43822..44865)
                     /gene="hrcA"
                     /locus_tag="ON449_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3933495.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(45100..46266)
                     /locus_tag="ON449_000887"
     CDS             complement(45100..46266)
                     /locus_tag="ON449_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3933496.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(46282..46509)
                     /locus_tag="ON449_000888"
     CDS             complement(46282..46509)
                     /locus_tag="ON449_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3933497.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(46896..47152)
                     /locus_tag="ON449_000889"
                     /pseudo
     CDS             complement(46896..47152)
                     /locus_tag="ON449_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(47495..47960)
                     /locus_tag="ON449_000890"
                     /pseudo
     CDS             complement(47495..47960)
                     /locus_tag="ON449_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        13613 a        10468 c         8381 g        16038 t
ORIGIN      
        1 aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata agactcagat
       61 atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg ttagcctgtt
      121 tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa tgagtcggga
      181 acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat ttttcgtggg
      241 ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag tagagggaca
      301 gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt tcattggcgg
      361 tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta tacagcttgg
      421 cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag aagaaaagat
      481 tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc ctatcgcagg
      541 attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt ggctgacatc
      601 atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct tgagacctgt
      661 tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct tagtgatctt
      721 aggagtactt ctaggcatct taggttttat ttatcagaag gtcttgctgg ctttgccaaa
      781 attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt tcttgctgat
      841 tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa tcgttttagc
      901 ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc ttcgattcgt
      961 tttttccatg atttcttacg gtggtggtct tccagggggg attttccttc cgatattgac
     1021 attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg ggatggatcc
     1081 aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag cgattggaaa
     1141 agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta atcatctgat
     1201 gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg gcggtaatcc
     1261 aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct cactaaaagg
     1321 tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg gattaatggt
     1381 acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag gaaatcagga
     1441 aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta tcttgactga
     1501 tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta tcacaaattt
     1561 gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc atctacacta
     1621 cttgattcta tatgattcta taaaaaaaga agagactggg aaaaaccagc ctcttctttt
     1681 ttatttctat gcttctgctg ctgcaggagc ttttctcaat aaaccataaa tcacaccaga
     1741 tacgactgct ccgatagcaa taaacaccaa atacatgacc gggtgactca gtaagaatac
     1801 aacgaaaatc ccgccatgag gagccagtaa tttgatcccg aaagctccga ctaatccgcc
     1861 agttaaagca gaaccaataa taaaacttgg aatggcacga atcgggtcag ctgctgcaaa
     1921 tggaatcgat ccttccgtga caaacgatag tcccatcacg atgttcgtca aaccagcatc
     1981 ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta gcaacgaaaa ttgccaatgg
     2041 aggtaccatt cctccagcca taacagaagc catcacgaca cttccgccag ttgcaacagt
     2101 cgtagctaaa gttgctgtac caaacacata agcggctttg ttgattggac cgcctaaatc
     2161 tgccgccatc attccagcta ataatgcacc aagtaaagca gcattcgaac cactcatact
     2221 tgtcaagaaa ccgttcaatg catcattgat tgctttcata gggacattga ccagcaacat
     2281 caagaaacca gtaatcagta agccaaacac tggataaaag aggatcgttt taattccatc
     2341 caatgatttt ggtaagttct tgaataattt acgtaaaaat acgatgacat aaccagctaa
     2401 gaatccacca atcagtgcac ctaagaatcc tgcaccgcct gtattagcta atgcaccagc
     2461 agcaaatcca acgatcaaac cagggcggtc accgatactt gaagcaataa atcctgctaa
     2521 taccggcagc atgaaaccaa atgcagcttg tccgatttgg ttgaaccagc tagcagcttg
     2581 attgtaattc cctagatttg ataattgatc atgtggcacg ccaataaatt ggtcgatcat
     2641 gaaagaaagc gcaatagcga ttcctccacc aataacgaaa ggtaacatgt gtgatacgcc
     2701 gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca tccgcatttc cttcttctga
     2761 actgctttct tttccatcac catggaatac tggtgcagta ccactaaggg ctagattgat
     2821 cagctcttct ggtttacgga taccatcact gacaggacga ttgatcagtt cttttccgtc
     2881 aaagcgattc atctctactt ttttgtctgc tgcaacgatt acaccttctg cacgggcgat
     2941 gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg ttcgtttcga ctttgatctc
     3001 cacacccatt tctttcgctt ttttcttcag agcatcttct gccatgtaag tgtgagcgat
     3061 ccctgttgga caagctgtaa cggcaacaac gaattttctt tctggatgag cagaagcttc
     3121 tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct ttttgctctt cagctgtttg
     3181 gaacaagtct tggacttctt ctggtgtatt tgcttctttt aattttccta cgaaatctgc
     3241 atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa tgtgtgtcgt ttgcgccttc
     3301 tggagctgcg atcatgaaaa acagataagt tggctgtccg tctaaggctt cataatctac
     3361 tcctttttgg ctttttgcga ataaaactgt tgcttcttta actgcactgt ttttcgcgtg
     3421 aggcatggcg attccatcac caagacctgt agaagtttgt gcttcgcggg ctagaattcc
     3481 ttctttatat gtttcgatat cggaaatccg gccggcatca aacatttttt ggaccatttc
     3541 atcgattgcg ccttttttat cagtcgcttg caaatccatg atcatcgctt ctttgaccat
     3601 taaatccttg atattcatga ttatcctcct tgaaactaat caatttttgt gatttcaact
     3661 tctggcagca actcatcaat caattcgcga gctgccaagt catcagaaaa agctgttgcg
     3721 cttccgcaag caactcccca tttaaaggct tcgattggat ctttcgtacg gctgtaagcc
     3781 ccaataaagc cggcaatcat tgaatcccct gcacctactg aatttttcaa tgggcgaact
     3841 aatacattcg ttttataagt tccttgttcc gtgaagagca aagcaccgtc tccagccatt
     3901 gagatcagca cattctgtgc cccttcagct agcaattttt taccatacgg aatgatttct
     3961 tctacagatt ggaaggttgt attgtaaagc tcagctaatt cgtgattgtt tggtttgatc
     4021 aacaatggtt tcttaacaag tgcatttttt aggtcatcac ctgttgtatc aatcacgaaa
     4081 tctgcacctt tttctcgaat gatatcaatc agttcttgat aaaatccttt tcgcaggcta
     4141 gccggcgcgc ttcctgacaa tacgacgata tctccttgtc ctactgttcc taatgcttgc
     4201 ttcaactcat cgatttcttc atctgaaata gatgggccca aaccgttaat ttcagtttca
     4261 gtatctgatt ttagtttgat attgattctt gtatcttctt ttacggttgt gaacaaagac
     4321 tggatttgtt ctttttcaag ccacttcgaa ataaattcgc cagtgaaccc tcctaaaaac
     4381 cctagtgctg tagattgaga atcgatccgt ttcaaaatac gggaaacatt gatccctttt
     4441 cctccaggta acttgaagtc ttccttcatt cggttcaaat cgcctagttt taacccatct
     4501 acgcgtacga tataatcaat tgatggatta agtgttactg tgtaaatcat tgtattgcct
     4561 ccttaattgt tgtcttttca cggaattcat taacgaattc taatggacaa cgctcggtta
     4621 tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga caactggttg aatttggtat
     4681 gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc taaacgttta acagctgcct
     4741 cttctgggtc cggagtcgtg aatccttgtt caagatgggc accgttcatt cccataaatg
     4801 ctttattaaa atgaaaattt tttagttgtt ctatcgcatt agatcctaaa acagcattag
     4861 ttgccagctt tatcgttcca cctaaaataa tggtagcaag ctccaaatca actaaatttg
     4921 ccgcatgttg aacggaattt gtcactactg tgatctgttt tccttgcaga aaaggaatca
     4981 tttctaacgt agtagagcca gcgtctaaat aaatgacatc ttcgtcatta atggtttcag
     5041 ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt gagtgatttt tcactcatat
     5101 tctgctcaaa attcaataat tgctctttct tagctccgcc atgaactctt tctagaaacc
     5161 cactattctc cagctcttgt aaatcacgtc ttatcgttga ttcagaagca ttaaaaaggt
     5221 ttacaagatc ttgtgatttc acgattttct gctgttcaag caagtctagg attttttgtc
     5281 tacgttcctc tgtaagcatt ttcacgatca cctcaataca aataatagca tatttttttt
     5341 ctactcgcaa tataaatcag tcaaaaacgt tcataaaaat tataaatcag tcaaaaaaaa
     5401 ataaaaaacc agtcaaaaag actggttttt tatttttttg tctatcatca agccgtttta
     5461 tcgtttttta ttttcgcttc ttgttttttt catataaaca gaagcaggtt ttctttcctt
     5521 atgaagaaga taataataaa gtcccatccc gccaaacatg accaaaaaac cgatgactga
     5581 tcgaatagga aaattaggaa caaaaaagag acagacggat aataatgatg ccggaaccat
     5641 tattcgataa tagagttgtt tatccatttt cttcacctca tattttttgt aaagtaatca
     5701 attgttctaa ttctcggtaa tctaaaaatt gcacacgcag atgctgctca cctttcttga
     5761 catcgaattg gatacttcgc cgctttttct ttattcgttt gaaggtaaca tcagagagtc
     5821 tatgtttgat ttcctttaac ggtaattctg acagggattc tgaaggaaca tagattctta
     5881 aggataaaag atactcttga tatttttcat ctgttacctc tacttctaga tattccctaa
     5941 tataataaca ataagcttcg attccattat ccaaagcaac agtcggatcg ataactttat
     6001 agcacatcat gcgcgagcga ttctcgtcaa cccaaggaaa atacgcctga gcatttagtg
     6061 ttccgcgaac acgcctttct ttataatctc cttgttgaat atcataactt tttgttacaa
     6121 aaaatgaagc attcttttta ggaacaggtt gcatttgtgc attctgatac tggacagatt
     6181 ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg tttcaataaa ccgacaccat
     6241 tgtcccaagc aagatgatca aacccaagct ggtccctaaa ataggattga aacatttgat
     6301 tgtttggctt ttgccagtta aaggggtctc gataaagctg gatacatatg acttcgtttt
     6361 ctaattcctt cactttttct acttgttgga tgtcgagaaa aatcggttta gggatgattt
     6421 gatgataata gcgactataa tacatttccc aacacgaagt gaaacataaa ccttggtaaa
     6481 aaagatcgta tcctggaaat tggttgcaat cgactactac ttccccgttt tgttgtttca
     6541 ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa tagccgttga gagatctgtt
     6601 ttgtattgtg atagataaac tcgctataag ctcgcatata ggcgaacacc ccatgagcaa
     6661 tcattttttt ctgaatgaag tctcgaatca aaaattgctt ttctttgaaa acagagatag
     6721 acaatacatt ttttatcaat aattgattat cttccaagcg aaaactacat tcgttctcgt
     6781 catttcctac aactaaccaa ctttcgtctc ttaccgatgc tagcattatt ttttcttgaa
     6841 gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga gcggataaat gttggctgga
     6901 tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg ttttttttct tcttcattta
     6961 agtctgtgag ctggatagtc atcaataatt tatcttcgcc aatcattttc tcacctcttt
     7021 aaaatctttt tataatttaa ttataaccaa cgataaagaa aaactaaaac tttatgtctt
     7081 agaaaccaat ttggacgatt acatgataga ctaagtaaaa agaaacaaag aggtgtttac
     7141 aatggggttg aaatttgaac gtacagataa tttagataaa cttttcgaat catttgctgt
     7201 cgatccggaa aaaacagttg caccaaaaga agagatcgat cgatttctaa aaccacagcc
     7261 acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt aagctcaaaa aaagacttac
     7321 cagccaaact tttatgaaag agtttgactg gtaagtcttt tttattcttt ttatgattca
     7381 attgcttgtt cgattgctgc ccacgcttct tcttttccct gttttgtaac ggaagaaaag
     7441 agaataaacg tgtcattgcg atcaaaatcc aacgcttttt tgatttcgct ctcatgcttg
     7501 ttccatttac cacgagggat cttatctgcc ttagtggcaa caacaattac agggatatca
     7561 taatacttca aaaattgata catctggata tcttcttgag aaggcttatg acgcaaatct
     7621 actaaagaaa cgaccgcacg tagctggtct ctagtcgtta aatatgtctc gatcatcttt
     7681 ccccattttg ctcgctcagt tttggatact tttgcatacc catatcccgg aacatcgaca
     7741 aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct gggtctttcc tggcttaccg
     7801 gaagtacggg ctaaattctt tcgatcaatc aatgtgttga taaaagaaga tttccctaca
     7861 tttgatcgtc ctgccagagc tatttctggc aattgtgtat ctggatattg ttttggtgcc
     7921 actgcactta tgataatttc agcttgatgg actttcatgg tctctctcct taacaaggtg
     7981 atgaataaag caagcggctg tcgcaaaagc caaaatgact tttacggcag cctcttttca
     8041 tcaacccgct tttttctctg aatctttata gattactgtt ggctcaccag acaattcagc
     8101 tgcttctttt gtgataatca ctttttcgat gttttcattt gaagggatat caaacatcac
     8161 atccatcatg atttcttcaa tgatggaacg aagtcctcga gcaccggtat tgcgttcgat
     8221 tgcttttttc gcaatggctc tcaacgcttc aggttcgaat tcaagttcgg tattatctag
     8281 agaaagaagt ttttgatatt gtttgactag cgcatttttc ggctcagtca agatccgtac
     8341 cagatcatct gttgtcaatt tctctaaagc tgccataaca ggtaatcgtc cgataaactc
     8401 agggatcaat ccgaatttta gtaaatcttc aggaatgata tgctgcatga cactttcgtc
     8461 ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc ttttcgccca tacgattctt
     8521 cacaatcgtt tcgatgccat caaatgcacc acctacgatg aacaatatgt tcgtcgtatc
     8581 aatctgaata aattcttgat gtggatgttt acgtcctccc tgaggtggca cgcttgcaac
     8641 agttccttct agaattttca gcaaggcttg ttggacacct tcgccggaaa cgtcacgagt
     8701 aatagataca ttttcgcttt tacgcgcaat tttatcgatc tcatcaatat agatgatccc
     8761 tttttctgca cgttccacat tgtaatcagc agattgaagc aatttaagca aaatgttttc
     8821 tacatcttcc cccacataac cagcttctgt caaacttgtc gcatctgcga ttgcaaaagg
     8881 tacattcaat gtttttgcta atgtttgtgc taaaaatgtt ttaccagacc ctgtaggtcc
     8941 gatcaagcag atattgcttt tttgcagttc aacatcatct gtgttttctt gtgcttgctg
     9001 attgacacgt ttatagtgat tgtatacggc aacagctaac gagcgttttg cgcgatcttg
     9061 tccaatcacg tagttattca atacttcaag aatttctagt ggtttaggga cttcagtgaa
     9121 ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt tctttacata ggtcgataca
     9181 ttcattacag atgtagaccc ctggaccagc tacgatcttt ttcacttctt cttgggtttt
     9241 cccgcaaaaa gaacagcgta cggtctcatt gctacttgga ttgtcataca tggtcttcac
     9301 cccttaactt gcaaaaagta tgcaagaaac tatcaaagac tttacagtct tttactatca
     9361 taacatagtt tagtaaaaag aaaaagtgtt taacaaagga tcaactgacc tatacctttg
     9421 cattttttac tcttaacaaa aaataaggaa ctgtgacaaa atcgtgtcac agtcccttat
     9481 ttcgaataaa caattttcta gaagtagctt cttcagaaat gatttattac ttgatttcta
     9541 ttctttcgct tctgtcgctg tacctgtgat caattcgatt gcttttttca ttgtcacatc
     9601 atgttccagc atatcttctg tcaacacgcg tttgatttgt tcgattggca tgttgtatgt
     9661 ttctgataat tcttggattt ctgcattgat ttcttcttct gttgcttgaa gattttcagc
     9721 agctgcaatt gcttcgatca caagatttgt tcttgtgcgc atttctgctt caccttcgaa
     9781 ttgtttatgc aagtcttctt ctgtagaacc agttaattga tagtacattt caggagagat
     9841 accttgacgt tgcatgttgt tcaagaattc gtccattgaa cgatgtactt catcatgaac
     9901 catcacgtgt ggcaattcaa cgatttctgc gttttcaaca gccatacgga tcgctgcttc
     9961 atcttttgca tcgtccgctg ctttttcttt tgtttctact aattctttgc gatatttttc
    10021 tttcaattcg tctaatgttg atacttcgtc atcaacatct tttgcgaatt cgtcatctaa
    10081 ttcaggcact tcttttgctt ttacttcatg gattgtcact ttgaagactg cttcttttcc
    10141 agcaagatct tctgcttgat aatcttctgg gaaagttaca tttacatcta gactttcacc
    10201 tgatttatga ccaactaatt gatcttcaaa acctggaatg aatgagcctg aacctaattc
    10261 aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg ccatctacaa agccttcgaa
    10321 gtcgattact actgtatcgc cattttcagc tgcagcatct tctttgataa ctaattcagc
    10381 ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca gcatctgtta cttcacggtc
    10441 ttgtttttca acaactagat ctttatattg tcctaatttt acttctggtt tcactgttac
    10501 ttcagcagtg attacccagt cttgaccttt ttccatgctt tcaacatcga tttttggttg
    10561 agaaacagga tcgatgccag cttctttgac agcagcttca taagcttctg gtaatacagc
    10621 attcaatgca tcttcatata aagcttcttc accgtacata cggttgaata cttgacgaga
    10681 aactttccct ttacggaatc caggtacgtt caagcttttt ttcactttat taaacgcagt
    10741 tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat tttaaaacac catcattcgt
    10801 gccttttttt tcaaatgttg cagtcattta attccctccg agtttagaaa ttcattttat
    10861 acatcaaaaa cgtttcatgc atacctttaa atagtacact aaccctttca aattgtaaac
    10921 atattgaatg gatagaatcc ttgtttttca taacattttt atctttttca ctgataaaat
    10981 ctaatggaag aagatcattt gctgcaaaat tttttctctt ttttttaaaa aatcttgttt
    11041 ttcatcttct gtaccttcat acatcgtttt tcctgtacac gcctctacag tcagttgaaa
    11101 ccagcgcttc gggtcttcga tgtatgtatc atagacagga taaacaattg cagattgcaa
    11161 cgtgaattct tcatttaaca cggaactaag cagcgcatct tcattaccgt aacgatctga
    11221 aaatacacat aattgctggt aacttgcctg ctgctgcggc tttggcagat cttttggtat
    11281 gactttgact aacttttctt caatcgtcaa atagaaaaca ggatggcatt cgttcaattg
    11341 cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg acctctggcg atacaataag
    11401 tttactcgct gtttggataa actcatgata aggaagctgt tcgatctcat caatagacgc
    11461 aagctgacgt gcaggttcca acgaaggaag ttcctccgat ttattgtgga gtgccttgat
    11521 cttctgcctc tccatttgtc cggataattc ttctgcaaat tggatttttt caatgacccg
    11581 ctgctcgaaa gcttcataaa aatctgtact tgctatcaat tttctagcat ataaaaaatc
    11641 ttgtgtactg attaacaagt caaaataaaa ttcagcaaac tcctcatttt gaagatactg
    11701 tttttcatgc aagagtgctt gcttcaatgc ttcttttttc tcgtccaact cgtaaagaca
    11761 aaaaacaagc aggctattag cctcaaagct ttctttcaac tcgtaagctc gttgaaataa
    11821 ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt tgtgctactc gcattaaatt
    11881 ctcatatttt tctggaaaat caatcggctt cacgcagtgt cctcctcttg ctcatgttca
    11941 gctcatgttc aatagtagca aaacaaaaaa agcatgacaa ttaaaaataa ctgaagcacc
    12001 ctctaaaagc tagattttag atctgacttt tagaaggtgc tttcaactgt tactgctttt
    12061 tattgctcat cagctgttga cttcctggtt gaatgctttg atcagattga tcgtaacaac
    12121 acttgtatta gtgaaaaaat cgcctagatg ttgctttttc tgattaaaga ttgctggaag
    12181 ataccctaac atcagcaaag gaaattttag aatgaaccgg caagcacctt ctctaactaa
    12241 aacggttgac cagctaagtt cttcttcatc aaagctgatg actcgaatac caaagatcat
    12301 tttcccaatc gtttgaccat gattgaattt cgtcaataaa ataaaatacg ctagataaat
    12361 aagtaaggct gtgaatccgt agatactcag ataagaatct gaaaaattaa gccaacctag
    12421 atttttggca ataccaagtg ttaaacgcgt aatagctccg atacacaaaa gatcgaccaa
    12481 gaaagcaaac attcggataa aaaaacctgc atagaaatac ttaggaaaat catttgcttt
    12541 tggcggttca tttcttgtgt ccgtatactt tttccattta cgttgtttct ttttgatttc
    12601 ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc ttttgggaaa agttggagtc
    12661 aggtcctaca gcaggctgct gttgcttatc tttttcttgt tccttatttt catttgaaag
    12721 ctctgacatg cttactcacc tccgtaataa tacattgctt ttggtgattc cgctgtccct
    12781 agattctcaa gaacggataa gatctgacta gtctcagaag gcttcaatcc ctggaattct
    12841 gctagtttac ttcctaacca tgtactagca aacccagatg gcgtggaaga atattctact
    12901 agttccgcat cttcaagctg ttgttccttt cgcatagctt ttaaagcatc ttcaggaaat
    12961 cccaattcat cgactaaacc tacttctttt gcttggacac catcataaat ccttccatca
    13021 gcaatttttt tgacttcttc ttcagattta ttccgtccct cgctgacgat tgctacaaaa
    13081 cgactatatg cgctgtcgat atatgcttga agcaccgctt cttcttcttt tgtctctggt
    13141 cgcgttcctg aacccatatc tttcaacgct ccgcttttaa ctgttgtatc ttccacaccc
    13201 agtttttcta gaagtcctga ataattcaat cctgacatga tcacaccaat tgagccggtc
    13261 actgtttctt ctgttgcaaa gattttatct gcttgagcag aaatatagta gccaccgcta
    13321 gctgccatgt ttttcatgct gacatacatt gggatatcat gttctttacg aatcgtatcc
    13381 aatttcttag cgatttccgc actttcgtag acaccgccac caggagaatt tacttctaaa
    13441 aagatacctt tgactgttgg atcttcttca atcgccttca gttgttccat aaaattttta
    13501 tgattgtaac cttcagaagc aaataaacct gaagaccctc ccgcagcaat cgttccatct
    13561 actgataatt ttacgatttt ttgcgtactg tctccctctt ccaacacatt tgaagctagt
    13621 tcttcatccc catataacca actgttcaat ccctcaattt gatcgttttc ctgaggctta
    13681 gtcaaactag ctgaaactaa cgaaaccaca aataatcctg cagcaatcag aactgctgcc
    13741 caacgtctct tattcattct ttctcctttc taatacaagt tttttatgac gatcacagtt
    13801 tctgactgct cagaagtgtc gaaccgctct gctttttctt cccgcgcata cccggaaaac
    13861 gactcttttg tcaattcatc ttctaccgta aactcacgag cttcagcaac ttccaaggca
    13921 tcaggtgcga tttcttggat gctatcccag ccagtatcta aaaatatttt cgtatcatct
    13981 gatacaccag ctgatgcttc gaatttggac aattgttttt ttaacattcc cagtgtcatg
    14041 atctcaattt ttgaatccat ttttactcct cctctatccg attactttct attgtaccga
    14101 actcctgaaa aagatgatag ataaaaaccc attatttttt gacataaaaa gagcatttga
    14161 caaagcattt gatatatacc aatttatttt ttattctttt tttgttgaca agaacattcg
    14221 caaaaggtta tgattttaaa tgtttccaga taaaaaacaa aagaggagga tagttatacc
    14281 ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg agagggttta tcgaaagatt
    14341 cggcggatgg ccctagggac tgcactctac agaaaatcca caaaaaatat ctgcaaagat
    14401 aaaacaaaag gattttcctg cagctggaag cattcatacg aattcattat agaaagtagg
    14461 atgtttttta tgtgcggaat tgttggaatg attggattaa aaaatgtaac accaggattg
    14521 attgacggtc tagaaaaatt agaataccgt ggttatgatt cagctggtat ctttgtctca
    14581 gatggcacaa ctgattatct ggtcaaagca caaggcagaa ttcaaaattt gaaaaacaaa
    14641 ataaatattg atactacagg aaatataggt attggtcata cacgctgggc aactcacggg
    14701 caaccttctg aagaaaacgc ccatccacat acttcccaaa gtggacgctt cgtgctcgtt
    14761 cacaatggcg taatcgaaaa ctttgaagaa cttaaaattg cttatttagc aaatgaccac
    14821 tttattggtg agaccgatac tgagattatc gctcatctaa ttgaaacttt cgcgaaggat
    14881 actacaacgc aagaggcgtt tttaaaagct ttgcgggtca tcaaaggttc ttacgctttc
    14941 gctttgattg atcggacagc acctgatgtt atttacgtcg ctaaaaataa aagtccacta
    15001 ttaatcggac tcggcgatgg attcaatgtg attgccagcg atgcaatggc tatgcttgct
    15061 catacgaagg aattcgttga gattgaagat gaagagatgg ttaccgtcac atcagaaaaa
    15121 gttattattc aaaattttgc aggtgacatc gtagaacgtt cttcattcga agcacaagta
    15181 gattcttcag atatcgagaa aggtacctat cctttttata tgctgaaaga aatcgacgaa
    15241 cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa cagaagacaa tcgtgtctct
    15301 cttgatcaaa agctggtcga tacattgtct gatagcgatc gaatctatat cgttgcttgc
    15361 ggaactagtt accatgcggg gttagcaggt aaacagactc tggaaaaatt gacacagatc
    15421 cctgttgagg ttcatttagc cagtgaattt ggctataaca cacctcttct ttcaaaaaaa
    15481 ccattcttta ttttcttgag tcaaagtggc gaaacagcag atagccgcca agttctggta
    15541 aaaatcaacc gtctcggcta cccttcactt acgattacaa atgttgctgg ttctactctt
    15601 tcgcgggaag catcctttac actgctattg catgctggac ccgaaatcgc agtagcatca
    15661 acgaaagctt atactgcaca aatagctgta ctaactttat tagccaaagc tatcggtgat
    15721 aaaaaagaat ttgaaacttc tcttgtattc gatatcactc atgaattgag tttagttgct
    15781 aatgcgatgg aatcggtcat cgcccaaaaa gattatttag aagagttggc agaagtatat
    15841 ctcagcgata caagaaatgc cttttacatt ggacgtggcg ctgactacaa cgtttctctt
    15901 gaagctgctt tgaaattgaa agaaatcagt tatatccagg cagaaggttt tgccgctggc
    15961 gaattgaaac atggaacgat tgctttgatc gaagaaggaa caccagtgat tggaatcatc
    16021 agtgaagaag tgactggtgc gcatactcga ggaaacctga aagaagtcga aagccgtggc
    16081 gctaaaacac tagttattgt ctcagaggga ttagaaaagg aaagtgacca gctggtgcta
    16141 ccaactgtcc acccttatct aactactcta accacagtcg taccgactca attacttgca
    16201 tattacgcaa cgctattacg gggattcgac gttgacaaac cacgtaattt agcgaaatca
    16261 gtgacagtag aataataaaa gaacaaggac cagtgtcaga catctgacac tggtccttgt
    16321 tcttttattt tttatgaaaa tatctcttaa tccagtccga tttcttcttt gactactgcc
    16381 gcgattttat cgacatagta attaactttc tcatcagtag gcgcttctgc catcactcga
    16441 agtaatggtt ctgtaccgga aggacgaact aggattcttc cttctgatcc catttcagct
    16501 tctgcctctt caatcacttt tttgattgcc ggaacatcca tcgcaccgtt tttatcactt
    16561 actcggatat tcactaattt ttgcggatag attgttacct ctgctgcaag ctctgataat
    16621 ttcttacctg tctgtttcat gatattcagt aactgaattc ctgaaagcat cccatctcca
    16681 gttgtgttaa aatctaagaa aatcatgtgc ccagactgct cgccgccaaa gttataatcg
    16741 tttttgcgca tttcttctac aacgtaacga tcaccaactt gtgtaacgac atctttcaaa
    16801 ccgattcctt ccaccgcttt gtggaatcct aagttactca tgacagtcgt aacgatcgta
    16861 tcttttttca atcgattttt ttctgccaag tatttagcac agataaacat gattttatca
    16921 ccgtcgacaa tgtttccaag ttcatccacc gcaataatac gatcgccatc gccatcgaaa
    16981 gctagtccag catctgctcc tttttcaacg accatctcag ctaaacgttc tggatgggta
    17041 gagcctacgc catcattgat atttaacccg tctggatttg tgcccatggt ataaaaatct
    17101 gtttcaagat cggcaaacaa acgattaaca cttgttgaag ttgctccgtt tgctgcatcg
    17161 acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt gtacaaggaa ttgagaatat
    17221 ttcaataagc cttctggaaa ttcttctaca gttcccaatc cttcagctga tggacgaggc
    17281 aattcatcca cttcagcatc taataatgct tcaatttcta gctcttggtc atctactagt
    17341 ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt gtgctggatt atgagaagca
    17401 gaaatcatga cccctgcgct tgctttttgc aaacgtgtca gataagcaac acctggtgta
    17461 gagatcacac ctagctggaa cacttcgata cccaccgata aaagtcctga aatcagcgct
    17521 tcttctaaca tttgacctga gattcttgta tcccgcccta ctaacacgcg tgggcgtttt
    17581 ccttcttctt catgctggct taggacataa cctccgcacc gtcccagctt aaatgcaagt
    17641 tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt ctgttccaaa atatttaccc
    17701 atagttgtaa aattactcct ttaacttaat tttctgagga atttgttctg ctcatttgtg
    17761 ttgattcgct tgtttctgat gtttcttctg tacttgtggt cgagctgctt gtcccagatt
    17821 ccgaactgga agaaatggtt gttgtcgatg gttccgttcc tgaatcagca gatgaaatac
    17881 ttctactcgt acttgtattt tcttgtgtcc gactgctcgt cgaagcagag ctactgcttg
    17941 acgtttgcaa cacgggtgta atctccacac taactgattc gggcttgata cttaacccct
    18001 cttctactgg tatttcaatt ttacgagttg ttcgatgatt gatacctgag acattgatcg
    18061 gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct agagccgctt acttctgctt
    18121 ccagttttga aaaactgaaa tcatagcttt ctaccccggc agctggtgtt ccttgctgta
    18181 tcccataaag tcgtaccgac ttgcttggct tgatcgaatc agctctcact gttacttgca
    18241 ctggatcaga gacgatactt aatacttccc ctgctgcatt cagcgcctgt attgggacct
    18301 cactattaat cccatcatct gtgaccatcg aagcgtccac atcagctact actcgtttga
    18361 tttctgccaa tgttttgtca cctgtagtga tagagacttt tttaggacta acggaagtat
    18421 catcaagttc atatccagat ggtgtgaacg ttgaaggaat caacgtttcc acatcgaaat
    18481 cttttattac ctttttctca accgttactg taatagtcga tggttcgatc tttgctgtga
    18541 cagcactgct caaattttga atttttagcg gaacctcatg tgttccttcg cccagcttag
    18601 tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt tgcctctgca ttcaactgaa
    18661 ctcggtttgc actggaaagt ttgacagtca ctgcattttc atatccatgg atatagtagc
    18721 gctttgaatc ataagaaatc ttgattggca ccccagtaac tgtttgatta tacgcttcat
    18781 ttccagaaag cgtatttgga acggactggc catttgcatt gaagtacagc gcaatactga
    18841 acagcaatgc aataaaactg taaagaatgc ttgtccgttt ctcttttgag atcatttgcg
    18901 atcccctccc ttagtcatac cgtctaggaa agattgaatc acgtttttct tgttttttgt
    18961 tttctcttca gcaatcagtt ctctacgcaa aattgccaaa tattcttgct gggataagcc
    19021 agccatcagt tcattatcta atgtaatgct gacatcccca gtttcttcag agacaacgat
    19081 ggtaatcgcg tcgctgactt cgcttatacc gacagctgcc cggtgtcgag tcccgaattc
    19141 ttttggaatc aacatacttt ctgatagagg aagataggcg cttgctactg cgatctttcc
    19201 ttcttttaca ataactgcac cgtcatgcag cggtgtatta ggaataaaga tattgatcag
    19261 cagctctcca gtgatatctg catctaatgc aattcctgtc tcaatatact catctaatcc
    19321 agtatgccgt tcgatagtaa ttagcgcacc gatttttcgc ttggacatat attggatggc
    19381 tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt tcgctttttg attgtttgaa
    19441 aagcgaactg cgtccaagat gttccagtcc tctccggatt tccggctgaa aaatcacgat
    19501 agcagctatc accccgtacg taatgacttg gttcatcagc caagacaagg tgtgcagacc
    19561 aatgatttcc gctacgattc ggataacaat aaaaatagct acacctttca gaagctgaat
    19621 cgcttttgtg cctcgcagca gttggatcag tttgtataca agataccaga caaccagaat
    19681 atcaagtatg ttcaccaaaa aattcagaga gaacaagtca ccggaaaaca tttgctgcca
    19741 atagtccaaa ttaaaaagtt ccccgatttg aaatgacata aaaagcctct cttctctttt
    19801 gagatttcct ctctagtata tcatatgttt taattttaga ggctgatctg ctcgtatttt
    19861 tcttgagaaa aacgcataat tttcaggaaa attgcaagta tgcgttttat ttttctctgc
    19921 aataaatatt tttaaacttc agagaattat tcattatatt ttttctgtta tttttccttt
    19981 ttttcttttg tagcttgttc cattttttta cgtcgttctg ctctggcagc tcttctttct
    20041 tctgcttcaa ggtccgtcgt tttttctttt ctagtcttaa cgggtacatc gatagattcc
    20101 tctggttcaa gtcgtttctt gattttttct aattctcccg tcagtgcagc atagttgtaa
    20161 aaacgtgttt tagcgtcttg gatagtcttt tcaaataaag cttttgcaag atttggattt
    20221 gcgttctcta gcgaggaaaa gcgaacttgt ttcctcatga aatcaggcat agcagagaaa
    20281 tcaggttttt tgtagtccaa agtcattggg ttcttgtttt tttcgaccat tgcagggtta
    20341 tatcgatata atgaccaata gccagaagaa acagcttctt ttgcttcttt aagcgattct
    20401 ttcattccac caagcaaacc atgtgtgata caaggcgtat aggcaatgat taaagaaggt
    20461 ccaggatact tttctgcttc ttcaaaagcc tttatcgtct gcatttgatt ggcacctgaa
    20521 gcaatttgcg ccacataaac atttccatat gtcatcgcca tcattcctag atctttcttt
    20581 gaagcatact ttccactagc cgaaaattta gcgattgccg aagctggtgt tgcttttgag
    20641 gtttgaccgc cagtattgga atagacttcg ttatccaaaa caagaatatt cacgtctgct
    20701 ccgctggcaa gtacatgatc gatcccgcca aaaccaatgt cgtaagccca tccgtctcca
    20761 ccaatcatcc attggctgtt ttttacgaat agatcctttc tcttgtatat atcctccagt
    20821 tcagggacaa ttgatcgttc gctttcgatc gcagcttgca gttttgctgc tctttgctgc
    20881 gttccttcgc tctcaaacat gtgtaccaac cagtcttcca tcaattctcg tagttcttct
    20941 gatacttttg gcaatacttc tttgattgaa cttgccaaag cctcgcgacg agtctgtgcc
    21001 gctagaagca taccataacc aaattcagca ttatcttcca ataaagaatt tgaccatgca
    21061 ggcccttgac cacattcatt ggtcgtataa ggagaagcag gagaagcacc gccccaaata
    21121 gaagaacagc ctgtcgcatt cgcgatcatc attcgatcac cgaacatttg tgtcaataat
    21181 tttacataag gtgtttcgcc acagcctgag caagcgcccg agaattcaag aagcggttga
    21241 ttgaattgtg tagacaagac agtattcttt cctttgactg gatttgcttt ttgtcttaat
    21301 gtcattgcaa aagcccaatt cacagcttgt tctttctgtt cttcataagg cttcatcact
    21361 aaagcttttt cttttgcggg acaagcctct acacacagac cacaacctgt acagtcttcc
    21421 actgaaactt ggatacggta ttttacgccg tctgcgcctc gcatttctct cacgataaat
    21481 ccttctggcg cttcttccat ttcctcttca tctgctaaaa atggacggat ggcagcatga
    21541 ggacaaacaa aggcacattc attacacatc gtacagcgat cactaatcca ctcaggtact
    21601 tctaaagcga cgccccgctt ttccatagaa gccgttccta aaggcatttc gccagttgtc
    21661 attccatttg ctacaagtgt accaacagag agttgatttc cttcctgtgc attcactggt
    21721 tcaagtattt cttgtatata accagtgttc cttttattga ttttttcagg catctccaac
    21781 gttctccaat ggtctgggat cgcgacttga tgaagcagtt cggtcgtccg atcgattgct
    21841 tgtatgtttt tttctacgat agccattgat ttatgtccat agcttttcag ggcttcttct
    21901 tttagcagcg gaagcacctc atcaaaagga atgatgtcag ctaatttaaa aaaagccgtt
    21961 tccattgccg tattgatccg gcgtcccaaa ccgacttctt gggccaatct catggcattg
    22021 atcgtataga atctaatgtt attttcagcc aagtaacgtt ttaattttaa gggcagatgc
    22081 gtttctaatt gttcgtcgct ccaaagagta ttcaataaaa aaatgccacc tggcttcaat
    22141 cctttgacca agtcatagct atgcaggtaa gctggtgtgt gacaggcaac aaggtccgca
    22201 tgctcgacaa gatacgctga acgtatcggc gtatctccaa aacgaagatg agagacagtc
    22261 agaccgccag attttttcga atcataataa aaatagcctt gcgcatattt atcggtgtga
    22321 tctccaataa tcttgatagc tgatttattt gctccaactg ttccgtcaga tccaaatccc
    22381 caaaacttgg cctggaatgt ttgcggttct gtcaaatcca gactttcctt tggttccaaa
    22441 gattgataag tcacatcatc cacgatccct atggtaaacc gttttttgcg tatgcttggg
    22501 tcttttttca attcatcaaa aactgctgag atttggtcgg gagtcacatc tttagacccc
    22561 aatccgtagc gaccaccaat aacagccggg cgaagttcag aatcatataa cgcactttga
    22621 acgtctaata agagaggttc tccacctgcc cctggttctt tacttcgatc aagaacagca
    22681 attgttttta ctgtatgagg caacttttgc acgaatgttt ccactggaaa aggacgataa
    22741 agatggatat tcaaaaaacc tgttttccgt ccgtttgcat tcaagtaatc aatcgtttgt
    22801 tcaatcgtct gtgcaactga tcccatagaa acaatgactt cttccgcatc gtctgctccg
    22861 taataagtca ccaagtcata agaagttcct cgtaatcggt tgatctcttt catatatttt
    22921 tgaacgattt caggtaattt atcataatag gggttgatcg tttcccgttg ttgaaaatgg
    22981 atgtctggat tctggttcgt accactaaca gtcggatgat tgggattcat ccctcgttta
    23041 cgaaatgaag ctaatttctc ctgattgatc agaggagcta attcttcata ttccaaaact
    23101 tcgatttttt gaatctcgtg actcgtacgg aatccatcaa agaaattcaa aaaaggtact
    23161 tctccttcaa tagaagcaag atgggcaaca ggtgctaaat ccatcacttc ttgtacactg
    23221 ctttcagcta acatagcaaa tcctgtttgt cttgcagcca tcacgtctcc gtgatctccg
    23281 aaaatattca acgcatttgt cgtcactgcc cgactagcaa catggaaaac acttggcagt
    23341 agttccccag caatcttgta catattcggt atcatcaaaa gcagcccttg agaagcggta
    23401 tatgtagttg tcagtgcacc agttttcaat gatccatgga ccacaccagc tgctcctgct
    23461 tcggattgca tttctactac ttttactggt tgaccgaata cattttcttt tcctgctgct
    23521 gaccattgat ctactaattc agccatggtc gaactgggtg ttattggata tattgcagca
    23581 atctcggtaa atgcataaga aatatatgcg gctgccgtat tgccatccat tgttttcatt
    23641 tttcgcatga ttcatcatcc ttgttcatat tctctcttat tatctgttcc attataacgt
    23701 aaaacattcc attttttctt ttatttataa aaatatttca catatgtcta tttgaattgg
    23761 tcatctaatt tttagacaaa cagtctaaat cgttgccttt ttttgtcaga catgtataaa
    23821 ctataagcga aagaggtgtt ttaacatgga tatatcagtg attgatgcaa cgaaaacaaa
    23881 tacacaaaaa ggaatccttt atggttcatc gaatgctcct aaaaaaatgg tcgaattcat
    23941 caacctatct tgtccgtatt gccgacaatg gtttgaagaa tcttatgagc tattagaaga
    24001 agcagtccaa agcgggcaat tgcagcgagt gatcaaattg ttcgataaag aaaaagaaag
    24061 cttgcagcga ggaaacgtga tgcaccggta tctcacaatc agcgatggtc aaaaagcgat
    24121 caaagagatc aaacaaatat tcgatactca agacgagtgg aaacatcttt ctctccaaga
    24181 agtagcggat tttgcagtag ataaactaaa gctgacagaa caaaaagacg aacagctgag
    24241 ccaagctgtc atcaacgaag ctgagcaagc acatattcga tttgttccga ctgtcatctt
    24301 gggtaaagaa atctttgatg aatctatttc aatagaggaa ttaaaagaat tgatccaaac
    24361 aaaataaaaa aataaggagc gtaggacaaa agtaaagaac acttttgtct cacgctctaa
    24421 acacctataa acggcggaag cagaagcaac tccttcttat ttcttggagt taaacgcttc
    24481 tgtcccagcc tccttattcc gaataaatgg tgtccaaaag cttgcatctg ccagattatc
    24541 tcatttctta tgacaagctg agcaaattat ctccaccaaa taatttattc atatttttga
    24601 cttgcgcata ctaactaatt cttaaatttt catacaaaat ttctcaaatt cccttaacga
    24661 gtaatattta tgtacggtga ttcacactag tatcccaatc actttttaag ttatcccgat
    24721 ttcaattcta cttcaaaaat agttgctttt atgtatgctg ttagttgatt agatggtcgt
    24781 ttgttgagct aggtcgttta ttttaaacaa gttgtttctt tagcttatac ctatctcgtc
    24841 taccaaacgc aagatggcaa acacaaatgc atacgcatct gtatttgcca ctttgattcg
    24901 tataaagcta acaactccta actcctctca tttaatagtt gctctctttt ttattcagat
    24961 acatttttgc tgatcacagc ctagtatttc aatggttttg aacactaaac caaattgttc
    25021 cattacaaca ttcactggct gatgaaatgg attaatagat ttcgattatt caaagcgatt
    25081 ttctattggt ggattgtgtt cccaactaac aagacggatt ccaaagtcat tatataactc
    25141 ttcaagagtt aaccctttcc tgttcccaat agatgtatct ctcccacgct cgatataaaa
    25201 tatggagttt cgaatgccaa gacctggcaa atctttcctt gttacagtta cactgctact
    25261 aacttcccca tataaaatcc agctcctcca aaatgataat atatttttcg accatcatct
    25321 aactcatgta acaataattg ttgtgccctc ttgtcactcg ccaaatgcaa acgccacgtg
    25381 cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc aacaattgta
    25441 tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc aaactcccct
    25501 cgggtaatcg caggtcttgg tggcttagga cccaatatta ttctacttaa aaccgcattc
    25561 gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt tctatttgat
    25621 aaatttctca ttattcgcaa atcacctcta cattctcaca tactagttcg taatactcct
    25681 ttaatatcaa aatatctact gatcgtaata atattcgcta tgaattccta ctactcgtat
    25741 aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc tatttatcgt
    25801 aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc aattcactag
    25861 ccttatatta tattgattta acaattcttc ttcatttatt gtattttgat caagcggacc
    25921 aaattgttct aataattctt tgtcatgtaa aatatccctt ctagtaatgc ctctttccct
    25981 ttcaatgaaa gaagcactag gaaaagtatg attataacca ttcaacctat ctcccatgta
    26041 ataattagca ggtcctggaa aataaaatat ttgcttatta tcactaactt ctaacagcaa
    26101 cactaaatct tctccgctgg ctaaacgact tgtccatcta cgatatctcc ctatgccttc
    26161 gttcattcca aatccatcaa attcaactat taacgtgtct tctatcacat gtaattcttc
    26221 attcaattca tactcaagtc taaaaggaaa ttctccatat gtaatctctg gcaatggtgg
    26281 tgctggagtt ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat
    26341 aaatgttatg agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt
    26401 tgctatatct agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa
    26461 acgcattttt gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttggta
    26521 gttcaagttc aggtataacg tattccaacc atacatctca attactattt atgaacttat
    26581 gattatcact gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg
    26641 gttgttttcg actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag
    26701 aatcttgacc agaagtaaga agagcagttc tcaaggatga tcgagcagtt ccttccagtt
    26761 ttttaattaa tcaggccagt tatagataaa tacttgatct tatctatatc aggacttcta
    26821 tattaagcaa aaatttatgt acaaaactaa aatcttcatt cagttgtctc cccgcttttt
    26881 atacgattgt attaaaaaca atagaagcct ttccaaatta tgttactcca gtaaagatgg
    26941 aattatagtt tgacggaaga aaagcaagta taacttttaa aaaaaactga tacattcgca
    27001 tctcctgctt catgtatcag ttttttcttt ggattaaact gcttcttcac gcagtttttc
    27061 cgcaaattca tttattttgc gaattcggtg attaattccc gattttgata tcgcacctga
    27121 gggaatcatt tcccctaatt ctttcaagct gacctctgga tgttgaagcc gaagttctgc
    27181 gatttcctga agtttttcag gaagggcatg tagaccgacc tttgcttcga tcaattcgat
    27241 gttttcgatt tgtttagaag ccgcatcaat cgtcttgttc agatttgctg tctcacaatt
    27301 gactagacga ttgaccgaat ttctcatatc ccgtacgatt cgaacatctt caaatcggag
    27361 cattgagttg gttgcaccaa tcaatgttaa gaaatcagca attttttctg ctccttttaa
    27421 ataagaaata tagccattgc gccgttcaag cgttcgagca tgtagaccgt aataatttaa
    27481 catatcgcaa atgtcttggt tatgctgttc ataaatagaa aatatttcca aatgataacg
    27541 gctcgtttcc ggattattga ctgaacctga ggccataaaa gcaccacgta agtaagaacg
    27601 cattttttgc gaatttccca taatctcgtc cgaaacgtgt ccattgaaca tcataccatc
    27661 catgatatca agatccatca acacatcttt tgtgccttgc ttcaaccgga cgatatagac
    27721 attgttcttt tttaatttca tttttttacg aacgagtagc tcacttcgta cctgataatg
    27781 atctttcaaa agagaataga tacgtctggc aattgcagca ttttctgttt ggacgttcaa
    27841 aataaactgc tggttcacaa tacctaaaga gccgttcatt cgcagcaaag cagctaattc
    27901 cgctttcgca tgttctcgat gaacttccaa accagttagt tctttcttca catctgcagc
    27961 aaatgacatc ctattctcct cctttctcca acaataaatt gcttaatatt ttgaaccgaa
    28021 aactaaacgg aacaattctt ctactacttt gtctccatcg tggaagacac caccatcttt
    28081 caattccaaa aagtcggtgg aaatcactcg acatccttca tttctcaatc cgttgaagtc
    28141 gtgtgcgact tgtactagat actcatcata gatctctggg tccatataat cttcaggcac
    28201 tttttccgta ttgaccaata ctgtatcgat aaaaggctga ttcaaatgct tgtgtaaaac
    28261 acgtatatga tcggcatctg tgaaatgttc cgtttcccct ttttgggtca tgatattaca
    28321 gatatagacg gtttcagcag ttgtcttgag cattgcttcg ccgatttctg gtatcactaa
    28381 atttggaagg atactggtga ataaacttcc gggtcccaaa acgaccatgt cggcgtccat
    28441 aattgctgaa acgacttttc gagccgcttt ggcttcactt ttatcatttg tgttgcagac
    28501 atacacacgg tcaatggttt tacggtcgat cgcgatttta gattctccta ctgcagtcgt
    28561 tccgtcttta aagacagcat gcaaggtcaa aggagtttct gaagaaggat aaacatgtcc
    28621 atctacatgc atcatttttg ctaataattg aatcgcctca taagtgcttc cccgcatctc
    28681 ggaaactgcg gcaatgatca gattaccgat cgtatgattg gctaaaaact gatcttcctt
    28741 tttgaaacgg tattggaaaa tatcttcata gaattgtggc atatccgata acgccactaa
    28801 gacatttctc aagtcaccag gaggagccat agcgatcgat tctctgattg caccgctgct
    28861 tcccccatca tctgcaacag tcactactgc tgtgatatct gctccttgat ttcgcaaact
    28921 tttcaggata actggcagac cagtgccacc gccgaccact acgatctttg gttttcttat
    28981 ccggtaggtt ttcattttca cgaccggttc accgtctctt tccttttatc cttgtcccga
    29041 tgtgtcacat tgactttata gtttttccct aaagcatttg ccaaacgttc agtcaatgcc
    29101 acagaacggt gttgaccgcc tgtacagccg attgcaactg tcaagctgct ttttccttct
    29161 ttaatgtatc caggcaatac agtttccaga agatccaaaa atttatggta gaatgattgc
    29221 gtttcatcaa aggacatcac ataatcatag actggctgat cttttccggt caatggtctt
    29281 agttccggga tataatgagg gttgggcaaa aaacgcacat ccatcacaat atccgaatcg
    29341 atcggcaacc cgtatttaaa accaaatgat acaaattcga tccgaaatcc ttggtctccc
    29401 tttgatttga atgactcatt gattttttca cgtaattgtc gtggtgtcaa tgtcgtcgta
    29461 tcaatgatca cggaagcttg ggctttcaag tcgtctagta tcgcccgttc tttacgtatc
    29521 ccttcagtga tcaaaccgtc catcgccatt ggatgtgctc gtcttgtttc tttgtagcga
    29581 gccactagtt cttcgtctga agcatctaaa aataagatag aagtgtcgat gaagttcgta
    29641 ttttcgattt cgatcagcat gttttggatt tcttcaaaga aggaacgaga acgtaaatcc
    29701 actcctaaag ccatttttgt gactttgcct gattctttga ttaattccca gaattttgga
    29761 atcaatcgag gcggcatatt atcgatacaa aaatagccca tatcttcaaa actttggata
    29821 gctactgttt tccctgcacc gctcatacct gttatgatga ctaattgcaa attatctgtc
    29881 ataattacga actcctctca cattgtgcat agtaaaatta tatcattccg ggcgtatcat
    29941 taccagtttt ctttttcttt tacaaaaaga tttggaccaa atatctttta ggatatttgc
    30001 cccaaatcca atttttttac ttttccgatg ctgtatccaa tgccttgctt ctttcagcta
    30061 gcgctcgttc aatcgtccga tctgctaaaa tagattgggc atcgatcact ggataatggt
    30121 tatcttcagc aaattcatgc atcaatgaca attctgtgca acctaagata actttttcac
    30181 agtttaggcg ctctaccgct tcttctaaga tttcgtaata tagctcttga ttcaaataat
    30241 ccgattcctt gatctcatga tagatcagat aattgatttt ttcttgaaga acagtatcag
    30301 gaatcactgt ttcgaatcca agatttttga cttcgcgttc atagattcca gctttcatac
    30361 ttccttctgt tcctagaatc gctactctcc ctgtcgtatg ctgacggaca agttcatttg
    30421 ctgcttctct tggcatatga aggataggaa tatccgttgc cgcttgtaat tcttcaaaaa
    30481 agtagtgtgc ggtgttacaa gtcaacacaa tgaagttcgg ctgcaataaa ttttgtttct
    30541 caatatcatc cagcagaaac ggcattggat tttcttctga tcgatctaaa atataagctg
    30601 tacgatcagg aactgttgca tgattgaata agacataatt caaatattct tgatcttttg
    30661 tagctttcgt tcggtggttg atcaagcgaa caaaactttc tgtcgccatc gtgcccattc
    30721 cgcctaaaat actgaaaaaa ttctccatga actagccttc tttcacatga aaatactttt
    30781 tgaatcttgg aatatagtta tggaacatat atttcatcaa caaccagcgc ttgattgacc
    30841 ggtctttttc ataaaacaca gtagtaccgt aacgattttc tttgatcatt tgctcagcta
    30901 gttttttgtc ttcattttcc cgagcatatt ctaaaaagat ttttttgggc acacccatcc
    30961 ataatcttgc tttatctgat tgattcgttc cataggtcgt ttcaacaaag ggtttgttaa
    31021 acactcgatc ttctgtgacg aaacgagcca gattcaagcc gttcaatgtc acaaagaaac
    31081 tgctacgacc ttgtctcaaa ttgatttcaa ataatttata ttctccatct ctaggatcat
    31141 acttcatatc gaaattggca aagcctgtat attcgatttt ttccaaaaat gccttaatgg
    31201 tttgatagat tttttcgtta taatctggca tgatcacaac gtaattacca atcgatgctg
    31261 gtgtaggatc ttctaacagt ggatgaccaa gacacatcat ccgaacttga tgatcttcat
    31321 ctacatacgc atttagcaca cgcatattac tatcatcacc cgggataaaa tcttgtacaa
    31381 tcatttcact tttataaccg gcttcgtata tccggcctaa aattaaatcg aactcttctc
    31441 tagtctctaa aataaaagct ttttttcttc cttcaaattg gacagaaaga tactccacac
    31501 tgtttgcagg tttcaaagca actgggaatt caaaaggcaa ttcctgttcc aaatgtccat
    31561 ttactaacat ttcttctctg acgatcaatg ttttaggata cggaagatca tattcttcac
    31621 aaacttcata aaagcttact ttattgatca gtctttcaaa taaagaataa tcaatatatg
    31681 gacagataaa atattctgac agctcttgtt tatgttggga gatcaattct gcatatccat
    31741 caccacaagc aatcaaaaga tattttttac tcttatcaga atatctttct tttgccaaac
    31801 gaagcatcgt ctcgataaac acagggtctt tatcaaatcc tggaataact tctacattga
    31861 caattttact gtagcgggta ggggctaact gatccgaagc atatgcttgg cagattttgc
    31921 cgtatgcttc attaaaagaa cgtgccatac cataaacgtt catgtcgctt cctaataata
    31981 tagggataaa ttcttcattt tcaatactgt tcatcattat ctctcactta ctcataatct
    32041 cttgtttttc ttttccaact acacaaggaa tatcatacca taaagaaaaa agcaatcaaa
    32101 ggaaactcct ccgattgctc aattcttttt tgaacggatt gaacttatag gaagtttttg
    32161 tctataccaa tcagtgtaga aagaactacc atgtaatgat tctagaacac tgatattaat
    32221 aaaacaagca aaaattcatt tttttatgtt tttttaacta tctcagctat ctatcctctt
    32281 tataccatta tttttttcaa atagtgacct gtataacttt cttttacgtt aatgatttct
    32341 tctggtgttc cagtcgcaag gatcgttcct ccaccttctc caccttctgg tcctaaatca
    32401 atgatataat ctgctgtttt gattacatct agattgtgtt caatcactaa aactgtgtta
    32461 ccagcatcta ctaatctttg taatacatgc aacaagcggg cgatgtcatc gctatgaagt
    32521 cctgtcgttg gttcatctag tatatagaaa ttctttccat tagagatttt gtgcaattca
    32581 ctggcaagtt tcatccgttg tgcctcacca ccggacaatg tcgttgctgg ttgccccatc
    32641 gtcacataac ctaagccaac atcaacaatc gtttgcagtt tgcgatgaat ctttggaatg
    32701 tgcttgaaga attctacagc atcttctact gtcatttcca aaatatcagc aatgcttttt
    32761 cctttgtaat gcacttctaa tgtttcagag ttatatcgtt tgccatgaca tacttcacaa
    32821 ggaacataga catcaggcaa aaagtgcatt tcgatcttaa taattccatc cccgcgacaa
    32881 gcttcacaac gaccgccttt tacgttgaaa ctaaaacgcc ctttcttata accccgcatt
    32941 tttgcctcgt tcgtttgagc aaataaccca cggatatcat caaatacact tgtataagtc
    33001 gctggattac ttctcggcgt ccggccgatt gggctttgat cgatatcgat gatcttttcg
    33061 atactttcgt agccggaaat tgtcttgaat ttacctggct tagcagaatt cttatttaat
    33121 ttttgcgcta acgatttctt taagatacta ttgaccaatg tactctttcc agaaccagac
    33181 acacctgtga ctgctacaaa ttctcctaat ggaaattcaa cactaatatt tttcagattg
    33241 ttttctgaag caccagtgat cttgattgct ttcccattac ctttacggcg ttcttttggt
    33301 acagggattg attttttccc tgaaagatat ttccctgtca aagaattttc atcattagct
    33361 acttcttcag gagtgcctgc agccacgatt tgtccgcctt gttctccggc cccaggtccg
    33421 acgtcaatca gataatctgc tgcccacatc gtatcttcat catgttcgac tacaacgagg
    33481 gtgttgccaa gatcacgcat tttctttaat gagccaatca gtcgatcgtt gtcgcgttga
    33541 tgcaatccga tcgatggttc atctaaaata tacaaaactc cagaaagatt cgatccaatt
    33601 tgtgtagcca atctgattcg ctgcgcttct cccccagata atgttccgga agcgcgactc
    33661 aaagttagat agtctagtcc cacattttcc aagaatgaaa gtctgtcatt tacttctttt
    33721 aaaatcggtt tggcaattac tttctcttgt tcagataacg ttaagtttga gaagaaatct
    33781 actgcttttt gaatagacaa ctcatttgtt tgcccgatat tcgttccatt gatttgaaca
    33841 gatagagctt ggggattcaa tcgataacct ttacatgtct gacaagttaa ttctgtcata
    33901 taaagacgca tttgctcacg cgtgaaatca ctgttcgtat catgataacg acgctcgata
    33961 ttcttcaata ccccttcgaa agtcgtttct acatcgcgaa cgccaccaaa atcattttca
    34021 taatggaaat ggaaaagatc atctgtccca ttcaagatga tatcctgatg ctcttccggt
    34081 aattcttcga atggtgtatc taaatcgata ccgaaacttt cacaagcctg cgctaacatt
    34141 tgaggataat actgagagct gattggattc catggaacga tagcaccttg actaagtgtc
    34201 ttcgttctat ctggaatcac taaatcttga tcgacttcta atttgacacc taaaccatca
    34261 cagtctggac aagctccaaa aggcgcattg aatgagaaca gtcttggttc tagttcgcca
    34321 acagtaaatc cgcaataagg acaggcatag tgttcgctaa acaacatttc ttcttcaccg
    34381 atcacgtcaa ccagagcata gccgtctgcc aatcgcagag ccgcctctaa agaatcaaat
    34441 agacgagaac gaatcccttc tttcacaatg atccggtcga tgacgatcgc gatatcatgt
    34501 tttttgtttt tctccagctc aggcacttca cttaaatcat agatctcacc atcgactcga
    34561 acgcggacgt atccctcttt ttggattcgt tcgaatattt ttttatgttg tccttttttc
    34621 tttaccacga tcggtgccaa cacttggatc tttgtgcgct caggcaaagc cattacttga
    34681 tctgccattt gatctactga ttgactggta atctcgatat gatcgttggg acaaatcgga
    34741 tgtcctacac gtgcaaacaa caagcgtaaa tagtcattga tttccgtcac agtacctact
    34801 gtcgaccgag gatttttact tgttgttttc tgatcaatag aaatcgccgg actcaatccg
    34861 tcgatgctat ctacgtccgg cttatccatt tgtcctaaaa actgacgagc ataagcagaa
    34921 aggctctcta cgtatctccg ctgcccttca gcgtacagcg tatcaaatgc tagggaactt
    34981 tttccagaac cggacaaacc tgtaacgacc acgaatttat ctcgtggaat cgttacatct
    35041 atatttttca agttatgggc acgtgcaccg tgaatcgtaa ttttatcgtt agccatgttg
    35101 tcctcctact ttgaagcttt taattctaaa atcatatctc gcacattagc ggctgtttca
    35161 aaatccaatg ctttcgccgc atttttcatt tctttttcta atttcatcaa taacgtatct
    35221 ttttcttctt tcgtcatttc ttcatacgag acttgtaccg cttcttttgt atcatcttcc
    35281 gattctttcg taatagaaat caaatctcgg atttctttaa tgatcgtttt tggaacgatc
    35341 ccatgttctt cattgtactt ctgctgaatc gtacggcgtc ttgatgtttc atccatcgct
    35401 agacgcatag aatctgttac tttgtctgcg tacatgatta ctttaccttc ttcattccga
    35461 gcagctcgtc caatcgtttg gactaaggaa cgttcactac gtaaaaagcc ttctttatcc
    35521 gcatccaaga tagcaacaag ggaaacttca ggtacgtcta atccttcacg aagcaaattg
    35581 attccaacta acacatcaaa ttcacctaat cgaagatctc gaatgatttc tgttcgttcc
    35641 agcgttttga tatcactatg caaatatttg actttgatcc ctaattcttt gaaataatct
    35701 gtgaggtctt ctgccatttt tttcgtcaat gtcgttacaa acacccgctg gtctttttcg
    35761 actcgttcat ttatttcgcc tactagatca tcgatttgtc ccatgattgg acgaacttcg
    35821 atcactgggt caagcagccc tgtcggacgg atgatttgtt ggataacggt atctgtttgt
    35881 tcatgttcat atggtcctgg cgtcgcagag acataaatga tctgattgac atgtttttca
    35941 aactcttcta accgaagtgg ccgattgtct aaagcagatg gcaaacggaa accgtaatct
    36001 actagcatct gttttctcgc tcggtcacca ttatacattc ctctgacttg aggcatggtt
    36061 acgtgcgatt cgtccgccac gatcaaaaaa tcttcaggaa agaaatccaa tagcgtataa
    36121 ggcggttcac cttctttacg tccgtccata tgccgagaat aattttcaat tcccgacgtg
    36181 tagcccattt ctaacatcat ttcaatatcg taattggttc gctgttctaa acgttgagct
    36241 tccagaagtt tgttttcatt tcgcaagaca gttaaacgct gttctaattc cgctttgata
    36301 ttcgctacgg catgctccat atgttcatca ttcgtcacaa agtgagtcgc cgggaagatt
    36361 gccacgtgtt ccgtttctcc taaaacttca cctgtcagtg cattgacttc tcggatacgt
    36421 tcgacctcat ctccaaaaaa ctccacacgt aaggcacgtt catctcttga agccggaaaa
    36481 atctccacga catcaccgcg cactcgaaaa cgaccgcgct gaaaatctat atcatttctt
    36541 tcgaattgga tactgactaa gtcacgaatc aactgattgc gatctagttc tgctccctgt
    36601 cgaatcgaaa ctacttgttt ttggtactca aaaggcgaac ctaatccaaa aatacatgaa
    36661 acggatgcaa tgacgatcac atcatttctt tctaataatg aactagtagc cgaatgtcgt
    36721 aatttgtcga tctcatcatt tacacttgaa tctttttcga tatacgtatc gcttgaaggc
    36781 acataagctt caggctgata ataatcataa taagaaacaa aatattcaac agcattgtta
    36841 gggaaaaatt ccttgaattc cccgtacagc tgaccagcca atgttttgtt atgggcaata
    36901 attagtgttg gtttattcac tttctcaata agattggaaa tcgtatatgt tttccctgtt
    36961 ccagtcgcac ctaataagat ttgtgctttt ttcccgccga ctacgccatc cactaattgg
    37021 ttaatcgctt caggctgatc accagccggt tggtatttcg agacaagctc aaagtgtcgg
    37081 gaggtatctc tctcaatcat tcttcgtcct ccttttcatt cattcctttt cattttacga
    37141 ccaataaaaa gaaaaggaaa gcaaaggcat tccttactaa aaaattatac cacaccgaac
    37201 atactttcgc cactttttta tcttattatg catgttacgt tttataacat aaaatgttag
    37261 aaactcgcat ttttttcatt ttttcgagct tacagtcttg tctgattcag cacttttcgc
    37321 ctataataat aaaatattgg aaatttagga ggttgattat gaaaagaaag catttatttc
    37381 actcatttgt tttcttaatg acaggacttg tgacacttgc gtttggacat gttgcacaag
    37441 ctgacaacac tacaacggca gagtcaagca caagcgagac aatgcaaaaa atagagccca
    37501 aaaaagacgt atacgtaatt gccagtgact ctgcttttgc tccatttgaa tttcaaaacg
    37561 caaagggcga gtacgaaggc atcgatgtaa aattgatgga aaacatcgcc aaactgcaag
    37621 gatttaacat ccgaatggac taccgtggtt tctctgctgc tttgcaagct ttggaatcag
    37681 gacaagcaga cggaatgatt gccggaatga ctgtaacaga tgaacgaaag aaaagctatg
    37741 atttttctga ttcttacttt gatagcggga tccaattagc tgtcaaaaaa ggagatactt
    37801 cgatcaagtc ttatgaagat ttgaaaggaa aaacagtcgg agcaaaagtt ggtaccgaaa
    37861 gtgctgaatt tttaagcgac catgaatctg aatatggcta ttctatcaaa tattttgatg
    37921 ctgctgatca attgtacgac gggttaaaag ttggcgcaat cgatgcaatg atggatgatt
    37981 atccggtaat cggttatgcg atcaaacaag gacaagaatt ggaaacaccg atcaaaaaag
    38041 aatctggcgg aaaatatgca tttgccgtta aaaaaggaca aaatcctgag cttcttaata
    38101 tgttcaatga aggattgaaa gaattaaaaa gaacaggcga atacgataag attattaaag
    38161 aatatgtagc ggatggttca gaaacttcca gtgagtcttc cactgctgat gaatcaacat
    38221 ttcttggact tatccaaaac aactatcaag cattgttgaa aggactttgg agaacaattc
    38281 tcctaacttt gatctccttt gctttggcta tagtaatcgg tgtgattttt ggattattca
    38341 gtgtagcacc aatacgcgct cttcgcacaa ttgctagtat ttatgtcgat atcatccgag
    38401 gaattccaat gatggttctt gcgttcttta ttttctttgg gttgcctggt atcgttggat
    38461 ttacgattcc tgacttcctt gctggtgtta tcaccttgac tcttaacgca agtgcttata
    38521 tcgcagagat tgttcgaggc gggatcaacg ccgttcctgt cggacaaatg gaagcttcca
    38581 gaagtttagg tctttcttat aatcggacaa tgcaaaaaat cattttgccg caagcgatac
    38641 gcatcatgat cccatcattc gtcaatcagt tcgttatctc attaaaagac acaacgatca
    38701 tttccgcaat cggtgtagtc gaattgctgc aaactggtaa gatcattgtt gcacgtacaa
    38761 cacaaagtac gtatgtttac ttgatcattg cgatcatgta cttgattttg atcacagcat
    38821 tgacaaaatt agcaaaagtt ttagaaaaga aggtgaagta agatggcaga aaaaatctta
    38881 gttgagcatt tagtcaaaaa atacggcgat aacacagtat taaacgacat caatgtgtcc
    38941 atcaacgaag gagatgtcgt ctgtatcatt ggcccttcag gttcaggaaa aagtacattt
    39001 ttgcgttgct tgaatcgttt agaagaacct tctgacggtg aaattattat tgacggcgcc
    39061 cacttgatgg ataaaagcac agatatcaac ttggtgagac aacatatcgg aatggtattc
    39121 cagcacttta acctattccc tcatctttct attttggaaa acatcatttt agcaccgatg
    39181 gacctgaaga aagaatctag agaagaagca gagaaaaaag caatcaaatt gctgcagaca
    39241 gttggtctag aagataaaaa agatatgtat cctgaaatgc tttccggtgg acaaaagcag
    39301 cgtgtggcta ttgctcgtgc tctagcgatg aaccctgaca tcatgctttt tgatgagcct
    39361 acatctgcac tcgaccccga aatggtcggt gatgtcttga acgtcatgaa aaagttagca
    39421 gaacaaggta tgacgatggt tatcgttacg cacgaaatgg gcttcgctaa agaagtggcc
    39481 aatcgtgtca tgttcattga cgacggaaac tttttagaag acggaacgcc tcaacaaatc
    39541 tttgaaaatc cgcaaaatga acggacaaaa gatttcttga ataaagtatt aaatatttaa
    39601 taaaaaccaa aaatgcccgg tcgcttcatg acgaccgggc atttttagtt tcttgttttt
    39661 tcacttaact gtaccagccg catcgcttgt tctcctgcac aaacaagcaa tcgctctacc
    39721 tgttccatcg cataagaaag taccatcggt tcattgacga tatcaagtac atagtcgata
    39781 ccaccttcag aaactatgat gattatctta ttctaaagct ggcttttttg ttggtacaaa
    39841 catgacaact acaaaaaaac caggcgaacg tatgattgtt cgtctggttt ttaattatta
    39901 atgacttagc gtttttacta gaaatagaaa atttgggcaa gtctacttta tatttttact
    39961 agaaaaacta cttccgcttt ttcccgccaa aagcatcttt aaacttatcg aagaaaccgt
    40021 cttcttgctg ttcttcaact ttgatgccgc cagcttcagc gaatgcacgt aatgcttctc
    40081 tttgttgttc atttagattt ttcggtgtga tcagttttac tttgacatgt tggtcgcctg
    40141 ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa acggaagttc gtacctgtct
    40201 gtgttccagc agggatcttc aatttcacat ctccatggac agttggtact tggacttcat
    40261 cccccaaagc tgcttgtaca aaacttaaag gcaattcata gtaaatttca gaaccttcac
    40321 gatcaaagat atcactttct tctacacgaa aaaccacata caaatcacca aatggtccgc
    40381 cgttttctcc agcttctcct tgaccagcta aacgcatttg ttgtccttct tctacaccag
    40441 ctggtacatt cactttaact gaatgtgctt tcttctcatg tcctgagcca tgacaggttg
    40501 gacaaggttc tttgatttct ttccctgtgc catgacaaac atcacaagtt tgacggctca
    40561 tcactcgtcc aagtggcgtt tggcgttcta cattgatcgt acctgcacca tgacatttat
    40621 gacaagtagt tggttgtgtg cctggttttg caccgtttcc accacaagta tgacagattt
    40681 cttcccgatt atacttgatt tctttttcta caccaaaaat cgcttcttca aaagacaagt
    40741 cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt tggatcgact gaccgaccgc
    40801 ctcctccaaa gaaagagtcg aagatatctt caaaaccacc gaagccgcca ccagagaagc
    40861 cgccgaatcc accaaatccg ccaccagctc caccagcacc gtaatttgga tctgtgcctg
    40921 catgcccgta ttggtcgtat gcagcgcgtt tttgcgggtc acttaaaatc tcatatgctt
    40981 ctgatacttc tttgaatttt tcttccgcat caggttcttt attgatgtca ggatggtatt
    41041 ttttcgaaag cttacgataa gctttcttta tttcatcttc tgatgctcct tttgagagcc
    41101 ctaagacttc ataataatca cgttttgttg ccataggctt tcctcctatt ggttctcatc
    41161 attttcacgt cgcttgttat catatcatat tttgtttgaa acaaaaaccg atacttcaaa
    41221 ctatttttca aaagaaaaag ccaaagtagc aactttggcc ttctctcgaa attcagtgaa
    41281 ctgattattt atcgtcgcca tcgacttctt caaaatcagc atcaacgaca tcatctgctc
    41341 cgccttgagc tgcttctgga ttttcttgag cttgttgttg tgcagcttgt tcgtatagtt
    41401 taacagtcaa gttttgaacg atttcgttca atgcatcgcg tttagttttc atttcctcga
    41461 tgttgtttgc ttcaacagcg gcttttaatt cgtcacgagc tgtttctgct tttttcactt
    41521 cttcttcgtc cactttgcct tctaattctt tcaatgtttt gtccacagag aatagcaatg
    41581 cgtctacatc attacgtagg tcaacttcct ctttacgagc tttatctgct tcagcgtttg
    41641 cttctgcatc tttcaccata cgttcgattt cttcgtctgt tagacctgaa gaagatttga
    41701 tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc agatacattt acaatcccgt
    41761 ttttatcaat atcgaatgtt acttcgattt gtggaatacc acgaggagct gctggaatat
    41821 ctgtcaattg gaatcttcct agagttttgt tgtcagcagc cattgggcgt tcaccttgta
    41881 atacatggat gtctactgct ggttgattat ctgccgcagt tgagaatact tgtgatttac
    41941 ttgttggaat cgttgtattg cggtcaatca atttagtgaa cactccaccc attgtttcga
    42001 tacctaatga caatggtgta acgtcaagta aaacaacatc tttcacatca ccagtgatca
    42061 cgccaccttg gatagcagca cccatagcaa ctacttcatc agggttaact gatttgtttg
    42121 gttcttttcc tgtttcttta cgtactgctt ctacaactgc agggatacga gtagatccac
    42181 cgactaaaat aacttcgtcg atttctgatt gtgacagacc tgcatctttt aacgcttgac
    42241 gtactggtac ttttgtacgt tctactaagt cagctgttaa ttcatcaaat tttgcacgtg
    42301 tcaatgtcat ttccaaatgc aatggtccag attctcctgc tgtgataaat ggcaagctga
    42361 tttgtgtgct tgatacgcca gataaatctt ttttcgcttt ttcagcagcg tctttcaagc
    42421 gttgtaaagc cattttgtct tgagacaagt caatgctgtt ttcttttttg aattctgcta
    42481 ccatgtagtc gatgattttg ttatcaaagt cgtccccacc tagatggttg tcgccagctg
    42541 ttgacaatac atcaaagaca ccgtctccta gttcaaggat agatacgtca aaagtaccgc
    42601 caccaaggtc gaatactaag attttttcgt cgcgatctgt tttatctaaa ccataggcaa
    42661 gagcagctgc agttggttca ttgacgatac gttctacttc tagaccagcg atcttaccag
    42721 catcttttgt tgcttgacgt tgtgcatcat taaagtaagc tggcacagtg ataactgctt
    42781 tttcgacttt ttcacctaaa tagtcttctg cgaatccttt gatatattga aggatcattg
    42841 ctgagatttc ttgtggtgta tatgatttac cttctacatc tactttgtaa cctgcttcac
    42901 cgatatggcg tttgattgat gaaatcgtat ttgggtttgt cacagcttga cgttttgcaa
    42961 cttcccctac ttggatttct ccatttttga atgatacaac agaaggtgtt gtacggtttc
    43021 cttctgggtt agcgatgatt tttgcttcgt ttccttccaa tactgctact gcagagttag
    43081 tcgtacctaa gtcaataccg ataattttac tcataattaa cgtctccttc tttaattgaa
    43141 agtgtaagtt ttatatccgc acaattattg tgcaacaatg accatagttg gtcttaacac
    43201 gcgatcatgt aatttgtagc cttcttgtag tacttctaca atcgtatcgg ctggtgtatc
    43261 ttcagtcgct ggaacagttt gtactgcttg atgaagatta ggatcaaagg cttctccttt
    43321 agcagggatt ttttcaattc cttcttcttc cagtgcgtta cgcaaacttt ccaagaccat
    43381 ttccacgcct tttttcaaac ctgccccttg atcatctgac acttctgtgg ctaatgcacg
    43441 ttccaggttg tcaattgatg gaagtaattt tttggctaaa tcttgtgaac ggtatttttg
    43501 taattgctca cgctcatttt ttcctcgatt tgccatattt gcgatttcag cacgagcccg
    43561 caagtattta tcttccattt cttctaactc agcttttaat ttcgctgttt cgaattcttc
    43621 cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg acatcgattt ccggttctgg
    43681 ctgagcatct gtcatttctt tatctagttc ttcttgattt tctttcacga tacagtcttc
    43741 ctttcattat tcagtcagct gtttgattgc tcttactaag aaagagagaa cgatcagctg
    43801 caaatagctt cgttattcac attacgagtt tgacaagtca agcgagcggt agtaatcagc
    43861 aagtttagct gctagttctc ttcgataaac atcgactaga ccgaacatcc gagaataagg
    43921 catactagta ggccctaaca aggctattgt acctcttcca tgaccatcta tctcgtaact
    43981 tgcttgaatc atactcatat tctgcaataa atcattgcct aattcagcac caatcttgat
    44041 atgaatattg ctatccatcg gagtgatcat ttgtgtaagt ccgtctgagt ctttcataaa
    44101 agaaaacatc gatttgaact gatcaagatc ttgacggggc tcataatcca agagattcat
    44161 ttgtccactg acataaacct tttcttcaaa aacatgacca agaacggtgt caaataactg
    44221 agacatccct tctgtcgtct ggaaatattt atgaagaatc atcggaatct ccgtacgaag
    44281 tttatggtac accgtcatca gaggatctcc gactagacgg tcgtttacaa tctgtaccat
    44341 tttttctagt tcctgacttc ccatatattc gggaagagtg aacacttggc tttctacatt
    44401 ccccatatct gttacgacga tagcaataat ctgtcgatta ttcaagggta cgatacgaaa
    44461 gccagtcagt cggcggtctt ttatttcagg tcccaatgaa aaagtcgtat agcttgtcag
    44521 ctcagataat atctcagcag attgcttaat aatctcattg atttcatgaa attctctgcc
    44581 tagagactga cgaatcagca gcaaatcatc ttctgctacg cgagcaggtt ttaacaaatg
    44641 atccacgtaa taacgatagc ctaaagcaga tggaatccgc ccagaagaag agtgagtctt
    44701 caacagcaat ccttcatctt ctaatgcttt catatcattg cgaatcgttg ctgagctggc
    44761 cttgactccc tcagacatta atgttttgga accaacgggc acgccactac tcgtatagtg
    44821 ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt aacatcttcc tcacctctca
    44881 ttagcactca tatattctaa gtgctaattc acttataaat atagcaagaa gcccttctcc
    44941 tgtcaagaga aagactccat tttttagcac tctaaacaca agagtgctaa ttctaaataa
    45001 ttgatgtaaa tccagttata tcaatacttc aatacctcat tgtcaaactt tctaaacttt
    45061 ttcttttcaa gtttcgataa aaaacaaata ttcttcaaac tatttttcaa ataaaaagcg
    45121 ctcaaacaca ttgttcccca aaaaagtccc tcttggtgtc aaataaacac ggtcttcttc
    45181 attaacaagt aacccttctt gctctaattc ccttagcaca tcaccgtaca ccgattcaat
    45241 tgttttttga tagcgatcat agaaatgttt tttagtaatc cctgtttttt ttcttagtcc
    45301 taagaacatt tcttcttcta tttgattttt tcgtgtcaat ttttcttctt ccaataccgg
    45361 gcgttcattt gctcgtaatg ggcgtaaata gtgttggatt ggtcctttgt tgcggtaacg
    45421 aatatctcca agataaccgc tagcaccagc tccaaaccca aaatagtggt cattgttcca
    45481 gtacatgagg ttatgttgag attcatgtcc ggagagccca aagttactta tttcatattg
    45541 gtgacgtcca tgcttttcca ttgcttcaat cgtctcttca aacattctgg tttccgtttc
    45601 ctgatcaggc agttcgagtc ttccttgtcg tacccagttc atgaacattg ttttgttttc
    45661 gaggatcaat gagtacaaag aatagtgagg cagatccaaa gccaaagctt tttccaaagt
    45721 atcccgatat ccttctagtg tttgtcccgg taatgcataa atcaaatcga tgcttacatt
    45781 ggtaaaattt tctgcttcta aaaattgcat tgtttgatac acatcttctg cagtatgttt
    45841 acgtccaatt tttttcaata agcgattgtc aaatgtctgc actcccatgg ataaacgatt
    45901 gacgccatag tttttcatca cttgaagttt ttcacgggtc aaatcaccag gattcgcttc
    45961 tacagtaaac tcattaccat tttgaaaagg caggatctct ctagcacctg ataatagtcg
    46021 atcaagttgt ttagcagata aagaagttgg tgttccgcct cctacataaa gtgtttccaa
    46081 attcgctgta ggatgacttt ccagcattaa tctcatttca ttcagcagca tttccacata
    46141 ctcatctact ggctgccctt ctaaaaaaac tttattaaag tcacagtaat agcagatatg
    46201 ctcgcaaaat gggatatgaa tgtacgcaga aaccttgttt tctgcgggat ttattgtttc
    46261 tatcattgaa attctttctc cttacgtcga aaagtcgtgc ctgttttcga ccttcttaaa
    46321 atcgcgttca agcaccaact cattgaaata agccgcatat ttagaatcaa ggaaacctag
    46381 ctctaaaaat gcttgcttat tgtcatcgga gctacacggt gcttttacag cctctcgata
    46441 aacggtaatc gcaaagctga ccttcggcat taagtcaaaa tctgcaaaaa tatgaaaagc
    46501 tattttcaca gattcctcct taagtgctca gaccacaacg ctttgcttac aacctctcat
    46561 atacgcatac taaacgtatt tggcgaacaa atccactaaa aaatccaaga atcgacttct
    46621 tggatgattt ttttacactc atgattcttc ctaaacaatt ttgtccttat tttattcata
    46681 aaaaaatcta gtcaaatctc tcacttcaag agttttaact agattccaat tattattttt
    46741 ttgtaaaaat aaacagtctc ttaatcatag acatttcttc ggtgccctac ttgaatagcg
    46801 ataacaacga attgattatc ttctatctct accaatacac gtatttgcca atcagagctg
    46861 cttctgaatt tcgggcgtga attttaaacg gcaggtcatt ccagcccacc aaattcatta
    46921 aggattcttc catgaatact gttgttttcc cacttcttat ccaatgttta tgggcaattt
    46981 cagccgtttg acgattgtat tcatcttcta gttgttccat cgaatagttt tttagaagtt
    47041 cagataaagt aatcccataa aattcagcca tataagaaag ccattctttt ttggcgtcac
    47101 ttacacgaac cgtaatcata gccctataat tgcctctctt aaagaatctc atgcatttcc
    47161 tgatgtgatt ttagaaaaat ttacttgcta aaaaactgtg aaaatgtagg agctagcatc
    47221 cttgtagtaa atgatcgagc tctttattat acattaaaaa agttcagaaa ttttgatgaa
    47281 gtagccaact actttttcat gagtaaagca acactagaaa aagaatcctc cagtacttag
    47341 aatttactgg aggatgtact tttgaagaag cctataggtt atttaaaaaa tattactaca
    47401 ggaaggaacg agaattactt cgttacttat aatagaactt agtcttatat agaaggcata
    47461 gctcacatta gcttatttgt cctataaaaa taaattacgg tattttagaa aagctatttt
    47521 ctcttttttc aacgatcttc agcaattttt ctttatcgtc aggtaactca agttcatgaa
    47581 ttctagcatg acaattggga cataacgcga tacagttttc aatcacatct tttccacctt
    47641 gcgacaaata ttctatatga tgacaatgta aataaggtac accgtctttt ataaatggcc
    47701 ctttttcttg gcaaagttga caaatacctt ttgcaagtcg tttaacatat tcagatacga
    47761 agggattacg tttatatatt tttgtttttg agaagcaaaa ctcctcttcg ttaacatatt
    47821 ttgcagccat ttttttatta taattactaa cttttcttgc ttcttcctct aatttagcat
    47881 tagataaatt acgaatatgt ctagtttgat tttcttcctg gttatatatc tttatcttca
    47941 atcaattgct cttgatttat taaagctaat ggaaatttat atacttttct gctgttgccc
    48001 gaactatctt tttcattaac agtatatatt tgtcctgcta aatatacttc tcccagatat
    48061 gtgtgttctt ttggtttaaa tgtttcaaat aaatgaatct ttacaaaatt actttttgaa
    48121 ttggctaaaa ctttattttg attcttatcc aatacttgat ccccttttag ccccataccg
    48181 gtatattgta aaataccatt taaccactta tctttataaa cattacttcc tgtttgatca
    48241 ctaaacagta caagagtatt agtttgatga gatcttctca ttccaccctg tttggaacat
    48301 ttaaacttct ctgaaatttc tttgttagta tatactctac caacttctaa ctcacctaat
    48361 atgtttttag accaattttc tcgccatgta ataatcttat caagattaaa catttctccc
    48421 tcttttggca tgccatttct aattaaatta gctaatacac cagtagaaat atctaatttt
    48481 tttagtaatt ctttcttagt
//
LOCUS       DAJSVP010000016        42900 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSVP010000016 DAJSVP010000000
VERSION     DAJSVP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.016"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            47..1387
                     /locus_tag="ON449_000891"
     CDS             47..1387
                     /locus_tag="ON449_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933498.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ON449_000892"
     CDS             1605..2048
                     /locus_tag="ON449_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3933499.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ON449_000893"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ON449_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3933500.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ON449_000894"
     CDS             complement(4373..5596)
                     /locus_tag="ON449_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3933501.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ON449_000895"
     CDS             complement(5627..6466)
                     /locus_tag="ON449_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3933502.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ON449_000896"
     CDS             complement(6483..7163)
                     /locus_tag="ON449_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933503.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ON449_000897"
     CDS             complement(7165..8238)
                     /locus_tag="ON449_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3933504.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8885..9556)
                     /locus_tag="ON449_000898"
     CDS             complement(8885..9556)
                     /locus_tag="ON449_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3933505.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9643..12321)
                     /gene="mgtA"
                     /locus_tag="ON449_000899"
     CDS             complement(9643..12321)
                     /gene="mgtA"
                     /locus_tag="ON449_000899"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3933506.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12980..13417
                     /gene="ndk"
                     /locus_tag="ON449_000900"
     CDS             12980..13417
                     /gene="ndk"
                     /locus_tag="ON449_000900"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3933507.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13439..14305)
                     /locus_tag="ON449_000901"
     CDS             complement(13439..14305)
                     /locus_tag="ON449_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3933508.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14465..16558)
                     /locus_tag="ON449_000902"
     CDS             complement(14465..16558)
                     /locus_tag="ON449_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3933509.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17307..17930)
                     /locus_tag="ON449_000903"
     CDS             complement(17307..17930)
                     /locus_tag="ON449_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933510.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18089..19486)
                     /locus_tag="ON449_000904"
     CDS             complement(18089..19486)
                     /locus_tag="ON449_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3933511.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19518..20489)
                     /locus_tag="ON449_000905"
     CDS             complement(19518..20489)
                     /locus_tag="ON449_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933512.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20863..21288
                     /locus_tag="ON449_000906"
     CDS             20863..21288
                     /locus_tag="ON449_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933513.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21776..22477)
                     /locus_tag="ON449_000907"
     CDS             complement(21776..22477)
                     /locus_tag="ON449_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3933514.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22493..22933)
                     /locus_tag="ON449_000908"
     CDS             complement(22493..22933)
                     /locus_tag="ON449_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3933515.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22933..23508)
                     /locus_tag="ON449_000909"
     CDS             complement(22933..23508)
                     /locus_tag="ON449_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3933516.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24356..24835)
                     /locus_tag="ON449_000910"
                     /pseudo
     CDS             complement(24356..24835)
                     /locus_tag="ON449_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24994..25695)
                     /locus_tag="ON449_000911"
     CDS             complement(24994..25695)
                     /locus_tag="ON449_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3933517.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25803..27212)
                     /locus_tag="ON449_000912"
     CDS             complement(25803..27212)
                     /locus_tag="ON449_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3933518.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27260..29125)
                     /locus_tag="ON449_000913"
     CDS             complement(27260..29125)
                     /locus_tag="ON449_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3933519.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29246..30097)
                     /locus_tag="ON449_000914"
     CDS             complement(29246..30097)
                     /locus_tag="ON449_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3933520.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30407..31120)
                     /locus_tag="ON449_000915"
     CDS             complement(30407..31120)
                     /locus_tag="ON449_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3933521.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31188..31514)
                     /locus_tag="ON449_000916"
     CDS             complement(31188..31514)
                     /locus_tag="ON449_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3933522.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31746..32963)
                     /locus_tag="ON449_000917"
     CDS             complement(31746..32963)
                     /locus_tag="ON449_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3933523.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(33045..33248)
                     /locus_tag="ON449_000918"
     CDS             complement(33045..33248)
                     /locus_tag="ON449_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3933524.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(33709..33939)
                     /locus_tag="ON449_000919"
     CDS             complement(33709..33939)
                     /locus_tag="ON449_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933525.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(33936..34358)
                     /locus_tag="ON449_000920"
     CDS             complement(33936..34358)
                     /locus_tag="ON449_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3933526.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            34863..35216
                     /locus_tag="ON449_000921"
     CDS             34863..35216
                     /locus_tag="ON449_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933527.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            complement(35276..35443)
                     /locus_tag="ON449_000922"
     CDS             complement(35276..35443)
                     /locus_tag="ON449_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3933528.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(35562..37481)
                     /gene="tet(M)"
                     /locus_tag="ON449_000923"
     CDS             complement(35562..37481)
                     /gene="tet(M)"
                     /locus_tag="ON449_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3933529.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(37497..37613)
                     /locus_tag="ON449_000924"
     CDS             complement(37497..37613)
                     /locus_tag="ON449_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3933530.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(37858..38790)
                     /locus_tag="ON449_000925"
     CDS             complement(37858..38790)
                     /locus_tag="ON449_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3933531.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(38787..39788)
                     /locus_tag="ON449_000926"
     CDS             complement(38787..39788)
                     /locus_tag="ON449_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3933532.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(39785..41962)
                     /locus_tag="ON449_000927"
     CDS             complement(39785..41962)
                     /locus_tag="ON449_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3933533.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(41965..42882)
                     /locus_tag="ON449_000928"
     CDS             complement(41965..42882)
                     /locus_tag="ON449_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3933534.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
BASE COUNT        12275 a         8980 c         7367 g        14278 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
     8881 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
     8941 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
     9001 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
     9061 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
     9121 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
     9181 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
     9241 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
     9301 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
     9361 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
     9421 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
     9481 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
     9541 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
     9601 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
     9661 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
     9721 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaacct
     9781 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
     9841 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
     9901 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
     9961 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    10021 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    10081 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    10141 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    10201 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    10261 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    10321 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    10381 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    10441 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    10501 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    10561 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    10621 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    10681 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    10741 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    10801 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    10861 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    10921 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt
    10981 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    11041 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    11101 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    11161 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    11221 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    11281 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    11341 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    11401 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    11461 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    11521 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    11581 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    11641 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    11701 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    11761 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    11821 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    11881 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    11941 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    12001 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    12061 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    12121 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    12181 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    12241 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    12301 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    12361 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12421 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12481 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    12541 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    12601 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    12661 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    12721 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    12781 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    12841 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    12901 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    12961 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    13021 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    13081 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    13141 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    13201 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    13261 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    13321 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    13381 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    13441 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    13501 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    13561 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    13621 ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca
    13681 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    13741 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    13801 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    13861 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    13921 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    13981 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    14041 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    14101 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    14161 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    14221 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    14281 tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat
    14341 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    14401 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    14461 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    14521 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    14581 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    14641 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14701 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    14761 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14821 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    14881 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14941 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    15001 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    15061 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    15121 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15181 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    15241 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15301 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    15361 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    15421 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    15481 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    15541 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    15601 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    15661 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15721 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    15781 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    15841 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    15901 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    15961 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    16021 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    16081 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    16141 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    16201 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    16261 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16321 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16381 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    16441 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16501 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16561 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16621 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    16681 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    16741 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    16801 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    16861 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    16921 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    16981 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    17041 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    17101 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    17161 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct
    17221 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
    17281 aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat
    17341 gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg
    17401 ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc
    17461 ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct
    17521 actttttcag aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca
    17581 tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa
    17641 atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa
    17701 taaatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa
    17761 gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt
    17821 tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt
    17881 aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc
    17941 actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa
    18001 gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt
    18061 tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt
    18121 ctttgaacca atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg
    18181 aaataaagcc atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc
    18241 acatatgata gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat
    18301 gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc
    18361 gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat
    18421 gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat
    18481 aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac
    18541 cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga
    18601 tacctaaaag atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag
    18661 gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag
    18721 ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt
    18781 cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac
    18841 caccttgctc catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat
    18901 agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat
    18961 aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac
    19021 caaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca
    19081 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg
    19141 gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt
    19201 cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg
    19261 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg
    19321 aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat
    19381 caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg
    19441 ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt
    19501 ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt
    19561 tcattttctt cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg
    19621 attttatctg ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct
    19681 aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa
    19741 cattcgatgg caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt
    19801 tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc
    19861 tctaattgga tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt
    19921 gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca
    19981 tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct
    20041 gctggtcctt ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca
    20101 aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc
    20161 aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag
    20221 ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg
    20281 ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta
    20341 gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc
    20401 ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc
    20461 atcttagcca catcacggat cgatgccata cattctcact cctgccagtt acatttgatt
    20521 tctctttctt gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca
    20581 atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa
    20641 acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt
    20701 tttaaaacgc tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt
    20761 tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc
    20821 caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta
    20881 ctatttattt tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct
    20941 gaccaatcct cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc
    21001 accaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg
    21061 acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct
    21121 tctgaatcat ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac
    21181 gaatatatta tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc
    21241 ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg
    21301 gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa
    21361 ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat
    21421 ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt
    21481 caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag
    21541 aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc
    21601 ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat
    21661 caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa
    21721 ggcttattct acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc
    21781 ctaattcttg accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt
    21841 ccaattcagc taatttttct tggatctgac ggatcacctt ttttgcatct tctggatttt
    21901 ctacaaaatc gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat
    21961 accattgatt atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt
    22021 ctagctgttc atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa
    22081 ctttgatatg gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta
    22141 gcatcgtatc taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat
    22201 cagcgtttag atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat
    22261 cgttcactaa agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata
    22321 aaaaagcgta ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat
    22381 tttcataaaa tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac
    22441 ctgcaccaat cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt
    22501 actcgcctac gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt
    22561 ctgattttaa atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt
    22621 cttgtacaat gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc
    22681 ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca
    22741 gttcttccat atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat
    22801 tgctttcttg gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg
    22861 ctttttcgat acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat
    22921 atatttgact cattattttg cctccttcaa taataactga tttttaatag atactgctcg
    22981 taattcattg accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt
    23041 atccataaaa tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc
    23101 gccaatattc aatattccga ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag
    23161 aagttgtgag aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc
    23221 cttttcaaat aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac
    23281 atcttttcca ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc
    23341 gttattttcc agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta
    23401 aaccagttcg atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta
    23461 actttcttta ttaccaaaac gggaaccgca aaaaacagta agtttcataa aaaaacactc
    23521 cctaactcaa aagtctatct tcattctaca cgtgtttttt taaaataaaa gactagattt
    23581 tgtagttttt tagtggtata taggatagac gccactatat atagtattta gaaaaaaatc
    23641 atttttcagc cattttatag ataaaacaaa aataaataac gtgttttcat cgatatgaaa
    23701 tgacttactt atagaagata ctgggatttc gtcctatatg ccaataacgt gtattatttt
    23761 aaacaacaaa tacttgtttc ttttattaac gaaaaaatca ttttgttctc gtgttatgtt
    23821 tcactgataa atgatgtttc tctaaataaa aaagccgacg aactaaacga tcaaaatttg
    23881 atcatttttg ttcatcagct tctcctaaaa atctttttgt taacgataaa tagctggatt
    23941 tgtaaaaata tcacttgaat cgtcactgct ggaagaaaat tcagtgataa ccgcacctgt
    24001 tgcgcctgcc tggaactagt gtttgatatt gggctcgaac gtgtactgat ctctagcatg
    24061 aagaataatt tcttttttta ctgtataata actttctttc ctttttggaa tagtacagga
    24121 aagttttttt gtttcctttc cttcgacata aggtatacca gaccctttcg acaacgaaag
    24181 gttctcattt ttccttcatc gtcatttcaa ggagggtgcc gatgctctgg acagtttcga
    24241 tcgctcctaa accaaaatct gtatattcga ttctttctaa ctctgattct ttcataccat
    24301 tcctgtctca aggtacatct attgtacttt tttcttgtaa tccattctgc ctctcctaac
    24361 taattgtcaa atcatcccaa gatatgtctt cttcctcttt ttcttcttgg atgatttctg
    24421 cattcggatc tactggtttt ttcaataata aaatggttat tcctgtgaca aaagaaccag
    24481 ctaacaatgc accaatgaat gcccatggtc tggtcatcgt agcgactgca aacattcccg
    24541 atgccggaat acggctatct gcgccccaaa acatgcttaa tccgccacct actgtcccac
    24601 cgactcccgt tgctatgaca ttgcgtgcca agtcgttcaa tacgataggg aaacatccct
    24661 ctgtgatctg acagatcccc ataggaaaag cggtcttcaa tgtatcgatt tcttgacgat
    24721 tgagtatatt tttacgaatg attttcccta agaaataagc gatcgtcaag ccgaatgggg
    24781 ttaccatcga agctaaaatc aatacagtga ttggttcata gattccttct gaaaatcgtg
    24841 ccacaccccc ttatccagaa taaatggtaa tccagaaaca gccgtgtatc tgcggtaata
    24901 agcctaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
    24961 gcagggcagc tttttcacaa cctctttttt tatttatgtt tcataatcaa tatggtaagg
    25021 atgcagctcc gtatggtaga ccgcctccga atattcaatc atttccccaa tttgcgtaga
    25081 cgatttccgt atccgtttga tgccgatctg tgcgtctgtt ttcagaattc cttgttcttc
    25141 ttctgttaaa aatactgccg agaaatcatc agaaaatgag tcaatagtat atcctttttt
    25201 cgtcagcaaa cgatacaaag attctttgcc aaattcttct ctgctgaatt gcatcatttc
    25261 ttgtggaaga taatgaacaa aataaatata aggctgattg ttcaaataat ataagcgctt
    25321 aaaacaaaca gcttgctcac cgaaaaagga aaacaaagga tcttccttct ttatatggac
    25381 caattgcaaa tccagcgttt ctttccggac ttcatatcgg gagttctcca agatttgcgt
    25441 aaagcttgcc gctttggata atcgattgta aggacgattg cttagaaccg tcgtcccacg
    25501 accacttttc tttgcgacat attcatctga tgccagcagt tcgattgcct ttcggacagt
    25561 gattttgctg acttgaaaca gttcttctaa ttcactttct gtcggcaaca tcgaaccgac
    25621 tggatatttt cctgagaaaa tatcttcttt aattttatct gcgacttcca agtatagtac
    25681 acgtgtcctt ttcataacgt caccctttgc tttactatta ctaaaagaat agcacataaa
    25741 aaataaaaaa agaacacaaa tcacatgtga gaagtgtgtt ctttttcatt cagctgcttt
    25801 aattacaagt ttacgccatt tgaggcaatt acttctttat accaatcaaa agattttttc
    25861 tttgatcgat tcagtgtccc atttccttca tcgtctagat ccacatagat aaagccgtaa
    25921 cgtttactca tttctcctgt tgatgcactg acaagatcga tacatcccca aggcgtatag
    25981 cccatcaaat ctacaccgtc tgctactgca ttagccatcg cttcgatatg acgacgtaaa
    26041 taatcgatcc gataatcatc aataatcgtg ccatcctctt ccatatgatc aatggctcca
    26101 agaccgtttt caacaatgaa taatggtttc tggtaacgat tgtataattc attcaatgcg
    26161 attctcagcc cttctggatc gatctgccag ccccattcac ttgcctcaag aaacggattt
    26221 tttactcctc ccagcaagtt cccttctgaa gttgctgctt gtggatcagt ctcattgatt
    26281 gccgtagaca tataataact aaatccgatg tagtccactg gatattcttt tagcaactgg
    26341 aagtcttttt ccgtttgctc taatttttcc ggatcgactc catatttttt atgggtacgc
    26401 gctgtgtatg cagggtattc tccacgtact tggacatccg tgcagaaaaa attgaattct
    26461 tggttttgga tcattgttgc tgcctgattg actggattgg catcgatgct gtaagttgtt
    26521 gcatagataa tcatgcagcc gatttggata tccgaacgca attcgtggcc gatttttacg
    26581 gctttgctac ttgcaacgaa ttgattatgc catgcttgga aaatattttg gtactctcct
    26641 gctccattgc tttttactaa tccttgactt aaagctggaa agtgaaaggc cgaattgatt
    26701 tcattgaaag tcatccaata tttgacttta ctgctgtatc tctctaatac tgtctgagca
    26761 aatcgttcat agaagtcgat cagttttcga tttttccagc caccgtattc ttttgccaaa
    26821 tgaagaggca tttcgtaatg agaaatcgtg atcacaggtt caatatcata ttttaagcac
    26881 tcatcaatca gctgatcata aaattctaat cccgcttcgt taggagtacc ttcgtctcca
    26941 tttggaaaaa ttcgtgtcca ggcaatcgaa aaacgataac atttgaatcc catttccgca
    27001 aacaaggcaa gatcttcttt gaaacgatcg taatgatcga ttcctcgatg attcgggtaa
    27061 atatattttt cttgatcaat cgtccagtca aattcttcac tgccgataat cgcaaagcgc
    27121 tgcttacctc caggcatagc atctgcaacg gatagtcctt ttccgtctcg atcataagcc
    27181 ccttctaatt gatttggcag cagttgctcc accccataaa aatttttctg aaaaacgtga
    27241 ttgcatcctg atttcctcct tattttacaa ttgccagaac atcttcgttt gcaataatct
    27301 ctttttggtc taattcaagt atatcggcaa atgcatcagt attcgtaatg acaactggag
    27361 tgattacgct atagccagct gctctaattg cttcgatgtc aaaacgaatc aataaatctc
    27421 ctgctttgac gagatcccct tgtttgacta agatctcaaa gccattccca tttaattcta
    27481 ctgtatccat cccgatatga atcaaaatct ccgctccgtc agtcgttgtg atccctacag
    27541 catgtccagt tggaaaaata gtcgtgatct ctccgtctgc aggtgcaact agttctccta
    27601 cttctggatc aatagcgata ccttttccca tcacgccaga agaaaaaact tggtctggta
    27661 cttcactcaa tggcagtact ttacccgtta aaggactttt taaggtatgc cgtgctgaag
    27721 taatcggttc tcctgcattc gcatgtttgt tttctaattg attgtcttgt gtttgttcgt
    27781 caaatcctaa aatcaatgta cctacgaatg caagaacaaa agcaattcct gtagctatta
    27841 ctgctactgt cacatttgac tctacaccat ccaccgtatt aatgaaagtc ggaatcgtca
    27901 ataagctgac caaactgctg gagaaagctt ttactccgga aaatccgata atagctccac
    27961 caatacctcc agaaatacaa gcggcaatga atggtttctt caaaggtaat gtcacaccat
    28021 acacagttgg ttcagtgatt ccaaataacg aagtcactgt agaagaaatc cctaatgctc
    28081 gaagtttcgt atcttttgtt cgcaatgcta cagctaaagc agccccacct tgtgccaaga
    28141 tggctggaag aagcattggc atcaatacat caaacccata ttgttcgata ttcaaaaaca
    28201 tgatcggtac gaatccccaa tgcatgccga acatcacgaa tacttgccat aaacttccca
    28261 tgattagacc tgctacgatt ggactaaatc cataaatcga gttgaagaga ccgcctaaag
    28321 cattaccggc aaccgtcccg attggtccta aagcaagaaa tgttaatgga accatcacta
    28381 ctaaaacgac aagcggaact aaaatcattt gcagaaattg cggaatcact ttttttacga
    28441 atggctctaa tttactttga acccatacgg ctagaatgat tggtatgaca gaagaagtat
    28501 aaccagatgg tgaaagaatc actgggatcc caaagaaact gatagcttct cccgcaccag
    28561 caactgctgt gatagctgga tacacaagtg ccatagcaat tgctactgct aaaaactggt
    28621 tggctttgaa ctttttagct gctgtaaaag caagcatgac aggtaagaag gtaaagacac
    28681 catctgctgc agcaaacaat atttgataag taccgctatc agctgtcagc cagcccatca
    28741 ccgttgccaa tgataagaat cctttcaata ccccggcggc tgccatcgcc cctagaaaag
    28801 gtgtaaatat actagagata atatcgatca gacgattcaa aatatttttg gactcttctt
    28861 ttccatcctc cgaaactgca agaccttcct catccacgat cgattgataa acatctgcta
    28921 cattactgcc aatcactact tgatactgac cgccactttg tacgacttgg atcacgtccg
    28981 ggtcttcttt taacttttgc gtatctgcta aagattcatc ttttaatctg aatcgcaacc
    29041 gcgtagcaca atgtactaaa cttttgacat tcttttgtcc tcctaccgct tcccatacac
    29101 gacgaccaac tgcttgatta tccattgttt tttccttctt tcttttttat ttaaaaaata
    29161 aaaacccaga ttgaaaaaaa cagtcacaaa cattcgtgaa cctatttttt caatccaggt
    29221 tttgcctgcc atacagtaac aatccttatg attggatgct ttttttctca cttactactc
    29281 tttgaatatg aatcgttaaa taaatctgct ctgcgatccc catttcctgt ttttgcgttt
    29341 gtcgaagata ctcattgatc cgttcgacac aatgaaacgc ttttggatac tttccttgaa
    29401 ctaatgcgta gagaaattca tcttgctcgt cagatccttc ccctttgaga taacgctggg
    29461 caaaaaactg cagatgagta atgatacgct gataattcaa agattcttca tcgaacacga
    29521 tgccgaagaa tttactgatg atactcagta tatctcttac cattgtggta gcggtcatcg
    29581 tcacttgcat gtcttcgttt ccttgttggc tattcacaaa atgaaaggca ataaaaccgg
    29641 cttcatcctc agaaagttcc acacccgtct tttgtttcac gaacaaaaga gcctctttcg
    29701 ctgcttgata ttctttcgga taaaattttt ttgtttcaaa taataatgga ttaggcagtt
    29761 ggatattttc ttttgctcgt gtgatcgcat aatgaagatg atcggtcaat gtcagataaa
    29821 tattaggcga aaattcaact tttagtttat cttcagccag ttcgactact tgtttgaccc
    29881 attcgatctc ctctgccgga atatcagcca tcagctgctg gaattggttt ttagcaaaag
    29941 aatctcttaa aacaaattct ttttggaccg cttctggcga aatctcgtcc ccaatctttt
    30001 tttgaaaagc tagtcctttt cccatacaaa cgacttcttc cccgaatcca tttttcgaaa
    30061 tgacgacatt gttattcaaa attttatcaa ttttcatttt gcttcgccct ctcaagaaaa
    30121 ataaaaaaac ctagaattcg cctactgttt cttagctgaa ttctggtttt gcctgccgta
    30181 cagtaacaat ccatttctta ttcaaaatca tcataccatc gaatttttat ttatgcaagc
    30241 cttttcatat aaaaaataaa aaaactggaa cacaagtcta tctagaaaac aaccaaatca
    30301 gagatgcacc tctttgcgtt tctctttttt tcagcaaatt ggtttttcct tatcgattca
    30361 tatcctttgg ctgtttttaa tctgtaacga cttatgtccc agtatcctaa aatgtttttg
    30421 tctctagatt gaaagcagat ctcatatcat ctgtcgcatc ttttatcgat gtaacacttt
    30481 ccagttctcc ttgtacaact agtcgcgtga cggcgtccat gtcgccacaa gtaattagtg
    30541 tgacaatttc ttttccctcg acttcattca gtaattgggt atctgtagga gcaacttttt
    30601 ctttaaaaaa tgttttgtat gtatacacat tttctaaatc tgtcaaatag atcttggcac
    30661 ccatctctaa attttctagc ggtgtaaaca gcaattcagg gttcgttgca cgatggcttg
    30721 ctaaggcata atttcctttt cccatttctt gatctggaga taatgttcct gctccgtaaa
    30781 gtagtgacgt attatccaag cctttgaaaa ccggtaaatt gattgaaact gaagggactg
    30841 cgatactagc aatcactggt agatctgttc ctctcatttg cgacctcatg accccttctg
    30901 tcgtcattgg ttctaccgca tcaaagtcaa aactgacatc tttatcgtta ttctttttca
    30961 gatcttcttt tgttacattc gctatagcat atttatctcc tgtctctctg acaaaataat
    31021 ccttgatctg ttcattgaaa atcaaagcta gtcctactaa taacaagagg aacaagagga
    31081 tattgactag ccatcttttt gcttttcctt tttttttcat tttctctccc tccagactca
    31141 ttttactgtt tttcttttgc tctttccaac agccgaatga cacatttcta caactctgtg
    31201 tatttttttc gtacacctgc gcttttttct acaggatatt tcaaattatt tttgatcaat
    31261 ttttcttcaa tgatctcatc tagatccagg tgaaggttgt ctgccatcat gtatgaatag
    31321 atcaatacat ctgccagttc ttctttgatt cgttctaatt gtgtttcttt tacttcttcc
    31381 ggcgttttcc attgaaaaag ttctaacaat tcagatgctt ccaaagaaat agagatagcc
    31441 aaatcttttt cgttatgaaa ttgtcgccaa ttacgttcat ctctaaactg gttgactttt
    31501 tccatactat tcatttgctt gccttcttcc aatcacttct tacttgatta atcataccat
    31561 ttttttacat aaaacaaagt ataaatttct aattatcttt ttatattttc ttaaatgctc
    31621 gtaaagcctt attctatgtg ctttcgagta tttttactgt aggaagatac ttcacgtttc
    31681 tttgcatatt tcctcatgtc ttagctgtca gaagtggtaa ataagtagta aattcatttg
    31741 tactactaag caacaagacg ctcctgttgc ttctctttat tcaagcgttt catttctgcc
    31801 attgcagaat cgaatgttgc atgtgcgtaa tagttcagcg tcatggctat attagcatgt
    31861 cccataatgt actgtaatgc ctttggattc attcctgcat ttgcatagtt ggtacagaat
    31921 gtatgtcgca aactatgtgg agtgatgtgt ggcaatttat cctcgttata cttattgtat
    31981 ttcttaacaa gacctttcat catgccgttg taatcacttg ccacttttgg atagttcttt
    32041 ctattaagaa agaggaaatc actatatcca tcaatctcaa cacgcttatc attctttcga
    32101 ttcgctaaca ctcgcttaaa tgcttgatag gcttcttcaa ccataggaac ttgacgttcg
    32161 ccacttttgg tctttggtgt ttcaatgtag tacccaattt cagtatctct caatagctga
    32221 tggtctatat tgacaagacg attctcaaaa tctaaatctg gaagtgtcaa accaccaaac
    32281 tctgaaatac gaagacctgt ttttaagagt atcagaattt catcataatt tttgctgtag
    32341 gttttatcag cttttgcaaa ggctaacagt ttttcttcct gttcttctgt tagtacggtc
    32401 ttagggacag tatcatcatc aagaactgct ttcagttgaa agtcaaatgg attcttccga
    32461 acacaatcat cttgtatagc aatatagaat gaagccttta aagaacgttt gtagttattg
    32521 atggtttgat aagcataacc attttcactc attctaatag cccattcttt agcgtctgat
    32581 ggcttaatac tgtcaatgct tcttacacct aacttgtctt tcttcaaaat atccataaga
    32641 tatttgcgtc cagtttcagt gttttttcta acctttggtc tttgagcgtt ctgttttgcg
    32701 taaagctggc agagtgtcat tttctttcct acaacatcaa taccatcatg aatgtctttc
    32761 tgtaactctg cgattttctc tctaagtgag atacaatcac gctttcctgc tggtactcgg
    32821 tctgtagcca caagtttcca cgagtaaaca aattgcggtt ctccaaatga atctatatat
    32881 ttgtataagt atcttccgtc ttttcgttgg ctctctccag tctttaagat tcgacctcta
    32941 ttgtcacgtc ttttttctga catggcattt gctcctttcc tttatggaaa gagccttgat
    33001 acgacttaat actattttat catatacaag accctttggc gacgctagat tgcgtccaat
    33061 gtatctataa ttttttcaaa ttgttttcgt ttaatctgaa tacgattgcc attcataatc
    33121 agccaatttg catttttatt ttcctctgcc aagcgtcgta gcttgttttc gccaatacga
    33181 aaatattttg acgcttcttc aatggttagg gtataacgtt cccaaatagg aatgtcagtc
    33241 tgcttcataa aatcctcctt tccaaatcac ttatttggat ttcataaaag ttgttttacc
    33301 agcaatcgaa cagctttagc aaagctcacg ggagttccac ccctgcatgg ttctcatgta
    33361 gccatactca ttgcctgcga cggttttatc acgctcggac tattgactgt atgggagtat
    33421 cattatcacg ataagaatgt cgttgcaggc aatcctgcta aagattgctt ctcggatcac
    33481 taacatgaat cgctcgctat ctttataaga taggtcatgg cggttagttc cgttggctct
    33541 tttcttatcg aaacgtattc gattactttt attcagtttt caaagaacaa tggctcgtta
    33601 gcctatcaaa acacattgaa agctcaatat gctttggtgg aataacaaac ctccctgttc
    33661 gggaagcgtg gaatggttta gcacgcttcc acgaaaggag agaggatatt acttaatttc
    33721 aaatgacaaa atctttgtaa tcagtctggt ttccattctt ccacgtaaga cttcatcaac
    33781 gaccatactt tgattgccat attcatcttt cataagtcgt agggaacgct tcgttatgta
    33841 ccctctgtaa tgatgtagaa tctggttaat cgcttcggta tcgccatctg ttgcctttac
    33901 aatgagagga aagggaatca taggatattg tgttttcatt cttcaaattc ctccataaac
    33961 tttttaatta aggctagtcc actggttcta tgccgataga cagtagaacg gttcaatttc
    34021 aacaggtctg caatttctga atcgctcatg tccataaagt aaaacagcag tagaatttca
    34081 cgtttcttgt ctggcaactc acgtaatgct tcactcaaca aatcattttc aacgcctact
    34141 gataacccat tgagtgtaaa aatctgaaag tcagttgaat agttatctgt tgtcgcaaac
    34201 tggctaacaa gataatcgcc aacatccgaa aaggacacct cacgctttgc aatccttgaa
    34261 agataaagca tataattctt tcgctcgtct tccatagcac gtttacagat atagtcaaac
    34321 tgattttcta ttgtggtctg aaaagaagat ggtttcatgt ttctcacccc ctttctgtct
    34381 aggaaaggaa gtgagccttg ctcgtttatc tcctttcact cttagtccca atgtgaaagg
    34441 gggatttgtt gcattactga taaataaact ttgtaaaaaa gttctgaata gccaaaaaag
    34501 catataaaca gatttatttc tctgtttaca tgcttctgtt attctatcta tatgatttat
    34561 aaaaccacat tggtggacgt acttatctat tgcagataga cgactttttt tgacaagaac
    34621 ccaatgtaag gaaatttatt gtatatgatg tacttcatgg cgacgttgac ctccaacaaa
    34681 ccgccatttg gaagtaatat acaatatttt aacagcgtaa atagcactac catataacgg
    34741 ttttttttat tggcgtttag tagtgctttt tattaaatat aaacctataa accatataac
    34801 acgtttttct atacctgttt ttaattcagt aggaacaata aaatgtatag aggtggtcta
    34861 ctatgcgtaa aaaagaagat aaatatgatt ttagagcctt tggtttagcc attaaagaag
    34921 ctcgattgaa acgaggttta actcgtgaac aagtgggagc attgattgaa attgacccac
    34981 ggtacttaac taatattgaa aataaagggc aacaccccag catacaagtt ctttatgacc
    35041 ttgtatcgtt acttcatgtt tccgttgatg aatttttctt acctgctaat aacttggtaa
    35101 aaagcacccg acgattacag atagagaaat acatggatag ctttacagac aaagaactat
    35161 ccataatgga atctttagcc agcggtatca acgaagcaag aaacatcgaa gactaattaa
    35221 aagaatccat acataacgga aagagccgat aaaatgagat tgtattaatc tcattttatc
    35281 ggctctgcgt ctttgcgtct ggctctgtaa tcacagttac tttgaactgc tttatttcaa
    35341 ttaaattttc ttgtctgcat ttcggacaat agagggggaa tttttttaat tcagtatctt
    35401 cccttatctt taatcgtgtt ttatttccac atacaggaca caatatccac ttgtagttta
    35461 taataactat ctcctccttt acactttaat tcaaatcttt attaaaaaat atttcatctt
    35521 atttaacaag aaaccatatt tatataacaa cataaaatac actaagttat tttattgaac
    35581 atatatcgta ctttatctat ccgactattt ggacgacggg gctggcaaac aggttcaccg
    35641 gtagtaacat ggtacccttt taactctgtt aaacaaacac tacgtccatt tgtaaagaaa
    35701 gttaaatcac tacgatattc ttgaatacac cgagcaggga tttctccact aagaatgacc
    35761 tcattatttt tcaattgagt gtctacgatg ttcgcacaat atttaggagc atcgttgtat
    35821 gctcgtgaaa gatattcctg tggcgcataa attttaaaac taagatatgg ctctaacaat
    35881 tctgttccag ctttttttaa gacttgttcc aatacaatag gagcaagcat ccgaaaatct
    35941 gctggggtac taacagggct atagtataag ccatacttaa aacagatttt acagtccgtc
    36001 acattccaac catacaatcc ttgttcacaa ccatagcgta tcccttccat aactgcattt
    36061 tgaaatgatt gatttaagta tccaagagaa accgagctct catactgcat tccacttccc
    36121 aacggaagcg gtgatacaga taaaccaatg gaagcccaga aaggatttgg cggcacttcg
    36181 atgtgaatgg tatattctgc attttttaac ggtctctcca tataaatgac tgtaggctct
    36241 tttagttcta tctccacatg atacttttct tgcaacagtg cactaatcac ttccatttgt
    36301 actttcccta agaaagaaag tataatttca tgtgtcgtag aatccacgta atatcgtaga
    36361 agcggatcac tatctgagat ttccaaaagg gcatcaagca acatttctct ctgttcaggt
    36421 ttactcggtt caacagttgt ttgtagtaga gggtgcggat tttcaatctt ttttctctgt
    36481 ggcaatagtt ttgtatctcc aagaacacta tttaacttca aaaactcatt ttgcaaaata
    36541 acaatttctc cagaataagc tctatcaatc ttacataatt caccatttat tgaagtatac
    36601 atttctgtaa cttttatttt ttctttttct gatactctaa ccgaatctcg taaatgtagt
    36661 actccactat aaaggcgtat atatgcaaga cgttgtcttt tttttgtata ttcaattttg
    36721 aaaacatttc cgcaaagttc agacggacct cgatgtgttg atgaataaaa tttattagta
    36781 ataacttcta taaggttatc aatccctata ttactttttg cacttccatg ataaagaggg
    36841 aacagagaac aattctgaaa tcttatgctt tcctcttgtt cgagttccaa tgcttctaat
    36901 gatttaccgg acatatattt ctctaaaagg tcatcgtttc cctctattac cgtatcccat
    36961 tgttcagatt cggtaaagtt cgtcacacac acattaggat acagttctac cttctgtttg
    37021 attacaattt cggcagaaag tttctcttta atatcctgat aaaccgttga taaatcaatt
    37081 ccattttggt caatcttatt gataaaaaag attgtgggaa tccccatttt cctaagtgca
    37141 tgaaataata tacgagtttg tgcttgtacg ccatcttttg cagaaatcag tagaattgcc
    37201 ccatctaaaa ctgataatga acgatatact tctgctaaga aatccatatg tcctggcgtg
    37261 tctatgatgt tcaccttcgt attttcccac tgaaaagagg ttattcctgt ctgaattgta
    37321 attcctctct gacgttctaa aagcgtatta tccgtcctcg ttgtaccttt gtccacgctt
    37381 cctaattctg taatcgctcc actgttatat aataagcttt ctgttaaggt agtttttcct
    37441 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
    37501 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
    37561 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
    37621 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
    37681 tgttcccgct atcaaattga cagtttattt aataatacct tgccgcatat ttattaactc
    37741 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
    37801 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
    37861 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
    37921 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
    37981 cttacgattg tgaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
    38041 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
    38101 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtcccctc
    38161 actttcaatg gcttttggtt tataacttga tttcttaggt atgttggtaa tggtcggatt
    38221 cttaaccagt accatatttc cagaaccatc tacatagaca ctcactatat aagcagagtg
    38281 gacggtcttt gtattttctc cctctgtaat gagctggtct acactgtagg ttacattaaa
    38341 ctcattgtcg ccagttggct ctaccgtcca tatctgaaat cctcttacag aagacgatac
    38401 aggaatatct ttgcgtactg tatcaacatt gagagcttga agttcatctg tcagatagcc
    38461 ttttagactt tccattcgat tatcaatgga cttatcggat tgctcccatg aatagtagac
    38521 tttcgcaaag ttctctacaa aattttctac atgatgagta tcaacgtatt ccttttctat
    38581 gatagttgtt tcgtgaatag tatgagtatc tatagctgta aagtgcttga atatcgcaaa
    38641 gctgaaacta agccctaaaa gtacccacaa ggcaatcaca acctttttat gaggattgac
    38701 cttatagaca cgaggtttct tttcctttgg tatctgtttt tctttattct gattttttct
    38761 aaatttcatc attaaatctt cctttctcat tgtttgattc gtcctgctcc cactaaatgc
    38821 tgttgccagt aggggcttgt taagtcggca taaccgattg ggtcgcctgc atgaaacata
    38881 cggttattgc caaggtatat cccaacatga gtaatataag agccagcgtt ataggtagaa
    38941 tgaaagaaaa ccaaatcgcc agcttgtgct tccgatagtg ggatatgctg ggtcacatca
    39001 tattgctgtt gtgcggttcg tggtaagtta attccagctt ttccatacgt ccattgtgtc
    39061 agtccgctac aatcaaaaga agtagtcggg gaagctccac cgtaaacgta tcgccagccc
    39121 tcatatttca gtgcttcgtc catgatggct tgtaccgtat catcatcaaa ctctgttgtg
    39181 acaagatact gcgttaccag ttgcacataa aacatattgc catagttgta tcgccagccc
    39241 ccattgatag gtatggctat gggattgggg taagacactt tttcgccacc tgaatactct
    39301 tttgagaaac tttgagccag ttcaaaggta tatttatttc cacgattagc cacataccct
    39361 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac actgagcctt
    39421 tcgctactgg ctaataattc actgaaatac ttcacacctt gcttaatgga ttcttctgta
    39481 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttccgcagta
    39541 ccgcccgatt ccacctgtat aatcgcaaga agtatgttga catattcttc aacgccatat
    39601 tctttggcat atttttctac cataggctta tgagccagca cttctgcgga aacattcaca
    39661 cctccataat gaatattgga aattccgctg tcctgttcat ctgaaaataa aatggcaaca
    39721 aacagaagca gtgagaagac catcaagaat aatccagaac caccaatcac taaagttttc
    39781 aacttcatgg tttcttaccg actttcttaa tggtggcggt tttgattggt ggtctacttc
    39841 ttgtattttg tagtggtact ctttgaacag tagacggacg ttcttttgtg attggacgtt
    39901 gtgaagttct atctgctgta gtggttgaag ttgctggctt ttgaacggtt ttttcttgaa
    39961 cggtattgcc ttggcgttcc acttttggac ttgaaaaatc ggacttaact gctggacgct
    40021 cttgtttggc ttgttgagat tccttatatg aagtctgaat attagactgt tttgaggtct
    40081 gttcatcatg atattgttct tgtcttgtag tcggtctttc atgaacagaa gaagcaggct
    40141 gttttttctg tttgacctgt tccatttcag agcgacgctt cgcaatggtt tttcgccttt
    40201 gttcctgctg ttccttgcgt ccactggctc tgtccgcttt ggtttgagaa atactactgg
    40261 ttaaatcacg gacattctct tttactttgg attttccttg atatactgca tatcttgcat
    40321 tggtcggcaa atctttaacc tgttctttca aaccactagc agtgtctacc attctgtctt
    40381 tggtatcagc tactgtaccg atggtttgac cgatacgttt tccaagtgtt gatttttcct
    40441 ttccgtctgg tcgggagtga tctgcttgtg tccttgcaga actccccgaa cccgactgtc
    40501 cttttttacc tgtaacaatg gcagacccag cccctagagt agtcatggaa cgtccaagtt
    40561 tccgctgtag acggtgcatg tgagcgtgca taagcatacg aggttttctc atcacacgac
    40621 ttcccacact ttgagaatcg ttactctgta gagaaaacat actcattaaa tcgcccagct
    40681 tgaagtagat tcctgcaaag gtcacaatct gtagaaaagc aatcaaaaag aacggataac
    40741 cagccgataa ggtatagagc atggttgaaa tactaaatgc tgtcgtaata atcaatgtga
    40801 ttccagctcg tgtcaaaatg gtattaaaga gctttgttat ggctcgtttt gacataccat
    40861 caaatgatgg aatcatgctt aaaataaagc tcacaggcag aaacatagca tagatgataa
    40921 aaagtacctg cgagaaaatc atgattcctg ttaataggaa tacaaatatg gaaatcccaa
    40981 tattgaagac aaataggaag aagactgtac ctaaacggtt aatggtcttt gtaatggtta
    41041 gattggtatt gcttctgtct tcaatttctt ccgcaacaat tttttctctg tcttcgccat
    41101 tgttggaatc tgggctggtg gagagcaggc tttccacacg gtcaataccg atactttcaa
    41161 tgtctgaact gttgtattga agcagtagcc acggttgctg aacctgtatg gaaaacaggc
    41221 tatctctgat taagtccacg ctgtccttgc cttgactatc ggaatggggc atgacaatct
    41281 tcgtgccaag tgataaactg gcattactga tgtctgatga aaagtcattg atttttttaa
    41341 tgtagtcggg agcgtaggca ataaaggaag ccgataggat aaacaccagc acaaaattca
    41401 taatggcatg aattgccttt gtggtttctc tctttatcag tcccgtatag gcaacataaa
    41461 ccccaagaac caaaatcaag agtaagagga atccaacata gaaaccctct gttgaaaatc
    41521 cgtttgcact cacaccagct aaggtctgca tattcttacc aatggaatct gctgtagcgg
    41581 aaatgaagtc taaggaatag gcttcctgta ctaagtaacc tgtcgcattg gaaacataca
    41641 aactgattgt ccaaataaaa ttggtaatgg catatagtcc atacatgacc tgttttccaa
    41701 tcccgtccga ccagttccac ggaagccagc cccagctatt atccacataa aaatccagtt
    41761 gatagttttc aagtgggtat cggctgtatt catttgccac attgaccgta tcatctacca
    41821 agcccgcagc ttgaaccacc gttcccagca tggctaaaag aaaaatggca atcacaagtg
    41881 tgaaagccac tgtcattgcc actttaccta gacgtttcag cgtccagttt gattttattc
    41941 tgtttactat tgatggtttc acatttacac ctcttttcgc acaggtggtc tggtatcaaa
    42001 ggcatggagc agttcttcaa atacagggtg gaactgtatc acaccgacac gaccatataa
    42061 atcactgata aggcattgcc cgttttccaa atcacgcaat cgcttctgat tgttttcgtc
    42121 ctctgggtct acaccaaaaa aggctaaggt ctttttaatc tcgttaaggt cagtggaacg
    42181 aaatgcaaat tttaagccga ggttattttt cagtttttca tctaagaggt cgtctgtatt
    42241 ttgggtcacg aaatataccc cagcgttcat agcacgacca gcccgaacca gcttcataga
    42301 tagtgttttt ccttgtgcta cctgtaaaaa gctccatgct tcgtctaaat ctacaatctt
    42361 gaaaatgctt cggtctgtat ggataaagtc taaagcaaag gtactaatga caatcagcat
    42421 agcaacggat aaaagctcca tagtggtata ttcctcaaag gaagtttcct tgtcgggaag
    42481 taccaagtcc gcaacctgta taatgttcag ttgtttttct aagctgatag actgctccac
    42541 ataaccatta ctgaataata aatgtgcaaa gtcatagtct gtaaaacttt cgatatggtc
    42601 ggctatactg gtacttagtg gcgtattctc aacccgtaat tcctcaatca ctttcatcaa
    42661 ccctcgtact tcactattgg ttactgcacg aatggctttt ctaaggattg ggaagcgttc
    42721 cccatcacga gaggaaatcc ccgtaaggaa tgtcagaata tcaatagcca gtgattcaga
    42781 atctttggga tttttcataa tcacataagg gtcaagtaag cctttgtttt tctcatcaga
    42841 agtcagagtg acgatattga tttcatggga aatctctggc aaggtttctt tccatctgcc
//
LOCUS       DAJSVP010000017        35047 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSVP010000017 DAJSVP010000000
VERSION     DAJSVP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.017"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            616..774
                     /locus_tag="ON449_000929"
     CDS             616..774
                     /locus_tag="ON449_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933535.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            846..2072
                     /locus_tag="ON449_000930"
     CDS             846..2072
                     /locus_tag="ON449_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3933536.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2160..2552)
                     /gene="rpsI"
                     /locus_tag="ON449_000931"
     CDS             complement(2160..2552)
                     /gene="rpsI"
                     /locus_tag="ON449_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3933537.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2568..3011)
                     /gene="rplM"
                     /locus_tag="ON449_000932"
     CDS             complement(2568..3011)
                     /gene="rplM"
                     /locus_tag="ON449_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3933538.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3530..3997)
                     /locus_tag="ON449_000933"
     CDS             complement(3530..3997)
                     /locus_tag="ON449_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3933539.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4117..5655)
                     /locus_tag="ON449_000934"
     CDS             complement(4117..5655)
                     /locus_tag="ON449_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3933540.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5735..6421
                     /locus_tag="ON449_000935"
     CDS             5735..6421
                     /locus_tag="ON449_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3933541.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6622..7149)
                     /gene="msrA"
                     /locus_tag="ON449_000936"
     CDS             complement(6622..7149)
                     /gene="msrA"
                     /locus_tag="ON449_000936"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3933542.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7233..7910)
                     /gene="pgmB"
                     /locus_tag="ON449_000937"
     CDS             complement(7233..7910)
                     /gene="pgmB"
                     /locus_tag="ON449_000937"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3933543.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7907..9037)
                     /locus_tag="ON449_000938"
     CDS             complement(7907..9037)
                     /locus_tag="ON449_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3933544.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9037..11220)
                     /locus_tag="ON449_000939"
     CDS             complement(9037..11220)
                     /locus_tag="ON449_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3933545.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11217..12242)
                     /locus_tag="ON449_000940"
     CDS             complement(11217..12242)
                     /locus_tag="ON449_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3933546.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12287..13114)
                     /locus_tag="ON449_000941"
     CDS             complement(12287..13114)
                     /locus_tag="ON449_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3933547.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13111..14178)
                     /locus_tag="ON449_000942"
     CDS             complement(13111..14178)
                     /locus_tag="ON449_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3933548.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14204..15040)
                     /locus_tag="ON449_000943"
     CDS             complement(14204..15040)
                     /locus_tag="ON449_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933549.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15037..15906)
                     /locus_tag="ON449_000944"
     CDS             complement(15037..15906)
                     /locus_tag="ON449_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933550.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15976..17259)
                     /locus_tag="ON449_000945"
     CDS             complement(15976..17259)
                     /locus_tag="ON449_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3933551.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17273..18958)
                     /locus_tag="ON449_000946"
     CDS             complement(17273..18958)
                     /locus_tag="ON449_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3933552.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19233..20258
                     /locus_tag="ON449_000947"
     CDS             19233..20258
                     /locus_tag="ON449_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933553.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20853..24527)
                     /gene="rpoC"
                     /locus_tag="ON449_000948"
     CDS             complement(20853..24527)
                     /gene="rpoC"
                     /locus_tag="ON449_000948"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3933554.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24585..28202)
                     /gene="rpoB"
                     /locus_tag="ON449_000949"
     CDS             complement(24585..28202)
                     /gene="rpoB"
                     /locus_tag="ON449_000949"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3933555.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28930..29898
                     /locus_tag="ON449_000950"
     CDS             28930..29898
                     /locus_tag="ON449_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3933556.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29916..30821
                     /locus_tag="ON449_000951"
     CDS             29916..30821
                     /locus_tag="ON449_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933557.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30818..31624
                     /locus_tag="ON449_000952"
     CDS             30818..31624
                     /locus_tag="ON449_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3933558.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31760..32749
                     /locus_tag="ON449_000953"
     CDS             31760..32749
                     /locus_tag="ON449_000953"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3933559.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33066..33590)
                     /locus_tag="ON449_000954"
     CDS             complement(33066..33590)
                     /locus_tag="ON449_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3933560.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33877..>35047
                     /gene="scm"
                     /locus_tag="ON449_000955"
     CDS             33877..>35047
                     /gene="scm"
                     /locus_tag="ON449_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3933561.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT        10063 a         7399 c         6548 g        11037 t
ORIGIN      
        1 tttatagttt agattttttt ctgttgctga agattagaat gacaaaacta tttgaaacta
       61 cgcgtatagt aagtagcttt caaaatatat aaaaaaggag cgttctcaaa tgagaacatg
      121 ctcctaaaac tactttattg gcgtatggaa aaattatact tgaagtggat agcgggttga
      181 ataagttgat aagcatcaaa acatttataa aaattattca agactatgtc tgttccatat
      241 acaaatatga aaatcagtaa actccattta acttttttcg tagtgtgtct ttttatacat
      301 gtttatcgtt attaatagtg aataggaaaa aaactatttt tgatcattga caattaaata
      361 caatttttga ataacgatcc ctgctattcg agctagaaac tcaagtacgc catgacctga
      421 ttagcgacaa atacttagag atttcttttt gttctgggaa aacaaaatgc catgacaata
      481 gcagcaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt gaaattccta
      541 tgtgcttacc caaagcaatt caaaaatttc ataactacta ttttaggaga tgattttata
      601 gaatccagag tctacctgaa taaaaaacaa acctgtgcat ttctaggaat cgataataat
      661 actctagata agtggattcg tatatataat ttcccagtaa taagagttgg tggtgtagtt
      721 cgctttgacg tagaagatat taaagacttt atgaatacat ttaaaataac ttgaaatttt
      781 cttctcgccg catatactaa aattagtgaa atatactgcc ttactttttt taaggagtga
      841 ggattttgcc tacaaagctt tcaaatggga aatataaaac taatctgaga tatccaaaaa
      901 ggtttaaaga aataacagga attgcatcag aaaagtatca aaaaacattt ccaaataggc
      961 aattggcgat taaagccgaa aatgatatga agaagaaaat agaaaaagtt ttgagagagg
     1021 agaatgcaaa ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt tacgaatcta
     1081 aatggcttcc tagatatgaa ttaggtcaaa caattagatc aaacaggcct cctagtgata
     1141 taaccattag taatacaaaa gatatatttc ggttgcatat tttacctatg tttggtgaat
     1201 acgcaatgaa ttatctaaat atcaatactg agataatatc agatgaatta accaaaaaat
     1261 cgaaagaata tgcaaatata aaaatcataa aaggatatgt tcgttccatg ttcgatattg
     1321 cagaaatact aaattatatc gaatttaatc gaactactaa aattattcaa agtatcactg
     1381 cacctaaaaa aaatgcttta gaagaaaaaa gaatacaaga aggtaagcaa gcactatcat
     1441 caaaagaact tactaattgg atagaagcag taaatgatga ttttaataat catttactta
     1501 cttttcatga ttacacactt tttatgatta ccctgtatct tggtgataga aaaagtgaga
     1561 cttacgcttt gcaatggaaa tatattgatt ttgaaaaaca aacggtccgt cttaaacata
     1621 ctttagataa atatcaaaga aaaaagttta caaaaggtcg aaaagatacc gtcattcaag
     1681 taccagaagt tgtaatgaca ttgctaagtg aatggaaatc ggtacaagct gatcaattgt
     1741 tgaaattaaa aattaagcaa acgttagatc agtatctctt tacttatact aaaccatctg
     1801 gagaagtaaa ttgtcctgtt catgccgatt acctaaacta tcgaatcaat tcaattaaaa
     1861 gaaggcatcc tgacttagtt catttatctc cacacaaact ccgtcatact tatgcaacta
     1921 tagctagaca aggtggagca gatatgaatc aaatttcaaa tgctttaaca cactcagata
     1981 tttctacaac aaagatatat gtgaacacac cagatatagt tgataaggct gtttttgaag
     2041 cttttcagag aggattgaat aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa
     2101 caaaagaagc actttccaaa taattgaaaa gtgccagaaa cgttgatata taaaggatat
     2161 taacgttttg agaattgtga agctttacgt gctttcttaa gacctggttt tttacgttcg
     2221 accatacgtg cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg gaagtcagga
     2281 tcaacttcta gcaatgcacg agcgatacca tgacggatag ctcctgattg tccagcgtat
     2341 cctccgccgt ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa
     2401 ggttgattga ttacttcacg caagtcagca tgtggaatgt attcttcaac atctttttta
     2461 tttacagtaa ttttgccagt tcctggtact agacgtacgc gagcaactgc gtttttacga
     2521 cggcctgtgc cgatatattg aacttgtgcc aatgaaattc cctcctatta gattaagttt
     2581 gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat gttctgctcc accgtatacg
     2641 ttcaattttg taaattgttt gcggcctaaa gtgtttttag gaagcatacc ttttacagaa
     2701 gtttcgatca aacgacgaga atttttagca cgcaattcgc cagcagtgat tgatttcaat
     2761 cctcctgggt agttgctgtg acggtaataa attttgtcag tcgctttttt acctgttaat
     2821 ttcactttgt ctgcattgat gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa
     2881 gttggtttat ttttaccacg tagtacagat gcaacaactg ttgaaagacg tcccaatgga
     2941 acatcagttg cgtctactac ataccattta cgttctactt cgcctggttt ggccatatat
     3001 gttgtacgca cgttattttc ctccaattta agttctgatg tttgacatac acttcgagtt
     3061 tccggggctc tttgtggggc aaacaatacc atttaacata ttacccgcta atctagtcaa
     3121 tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa ctttacttct tttctttctt
     3181 ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc
     3241 ttgtcacaac cccttatttt gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
     3301 cacaatatcc ataaaacata agaagctgtt ttttaaaatt gtttcttgtg actcggagct
     3361 gaacggcagg cttacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag
     3421 ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc
     3481 aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga
     3541 tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt
     3601 tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc
     3661 ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg
     3721 gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac
     3781 gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact
     3841 gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc
     3901 ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta
     3961 attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc
     4021 ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga
     4081 acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc
     4141 ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa
     4201 taatggatag attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt
     4261 caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc
     4321 taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc
     4381 cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg
     4441 gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca
     4501 gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat
     4561 aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg
     4621 aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata
     4681 tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc
     4741 ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc
     4801 cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg
     4861 aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc
     4921 tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc
     4981 tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg
     5041 cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc
     5101 atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa
     5161 caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc
     5221 aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt
     5281 cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc
     5341 cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa
     5401 caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg
     5461 caactgttta attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc
     5521 ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc
     5581 ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc
     5641 gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc
     5701 ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg
     5761 ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat
     5821 ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc
     5881 gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt
     5941 ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc
     6001 ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat
     6061 ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg
     6121 gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt
     6181 aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat
     6241 gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag
     6301 tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa
     6361 gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg
     6421 aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa
     6481 tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc
     6541 agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc
     6601 ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc
     6661 acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg
     6721 atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt
     6781 atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc
     6841 ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa
     6901 tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac
     6961 ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg
     7021 ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc
     7081 tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt
     7141 ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa
     7201 cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa
     7261 tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa
     7321 ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga
     7381 caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt
     7441 gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata
     7501 aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt
     7561 gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca
     7621 ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt
     7681 tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac
     7741 agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca
     7801 gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct
     7861 gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc
     7921 cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat
     7981 cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg
     8041 ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta
     8101 tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac
     8161 tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat
     8221 cgaactccag taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg
     8281 ctaacagtcc tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag
     8341 gggcaaatct catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt
     8401 tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag
     8461 taacatcagt aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat
     8521 caactgtagc aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat
     8581 cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc
     8641 cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc
     8701 ctactcctcg cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc
     8761 cgacgatttc tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt
     8821 tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg
     8881 ctaatgatga aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct
     8941 gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac
     9001 tttctttgaa agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat
     9061 cagttctttt tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt
     9121 ctcttttttt attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca
     9181 aataaaagga aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt
     9241 tccattcgtt actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa
     9301 tgaagcagca tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca
     9361 cgcttcttgg aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc
     9421 gtgaatcgct ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt
     9481 cttagaaacg attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc
     9541 tgcttgcttg ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga
     9601 ctggctgcca ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt
     9661 gtcatcttgc ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa
     9721 attttgacat tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac
     9781 attataatgc gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc
     9841 tggaccaatg actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca
     9901 aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc
     9961 tgttgcttgg acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc
    10021 ggcagctgaa gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg
    10081 tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata
    10141 atctcttgct ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt
    10201 tgctacttca ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa
    10261 aacatgccct ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc
    10321 attgcgggga gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa
    10381 agaagaatcg ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat
    10441 cgactgctct aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt
    10501 aaacgggtct gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga
    10561 cgtatttttt ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa
    10621 tacacctgct ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg
    10681 gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg
    10741 tgtaccaaaa ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc
    10801 ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac
    10861 acgacaagta atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt
    10921 aagctctccc atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag
    10981 agaaaaatgt tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa
    11041 ctcggctgat tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac
    11101 ttgattccga tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt
    11161 catctgactc gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat
    11221 aaggtaataa cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg
    11281 acatatcctt gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca
    11341 aaatgagcca tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc
    11401 agctttccat ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg
    11461 ccacaaaaag ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt
    11521 tggatgatct tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat
    11581 ttgcttggat cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag
    11641 ctgtacgccc gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc
    11701 atgtgaatgc cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt
    11761 ccccagcctg gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg
    11821 agaacatcaa gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca
    11881 taagccaaaa tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct
    11941 gtcatagctg gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca
    12001 tgttcgaagt gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc
    12061 agcatttgtg taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct
    12121 gctcgctggc tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga
    12181 taataagcag catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc
    12241 atttatttct ccctcctttg cttatttact tattgttatt ctttctctat tctttctcta
    12301 aatcatttaa atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat
    12361 aaattttcac ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact
    12421 cattttgaag tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt
    12481 agcgatgatt atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt
    12541 ttgaaatatc atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt
    12601 tcaattgatt agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt
    12661 ggtatcggtt caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta
    12721 aataacgaag tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca
    12781 tcggcggtaa tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc
    12841 caacctcgct tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt
    12901 cttcgataaa gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc
    12961 ctgtttcttt gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga
    13021 tttcgtattc ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg
    13081 ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc
    13141 cattttgata ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc
    13201 tgcaaatggc acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa
    13261 ttcccaacat gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc
    13321 actagatgct cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt
    13381 aaacccagct ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat
    13441 ccctcttaaa gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac
    13501 tccacgttta tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag
    13561 cgtgtaatcg gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc
    13621 aacgatggta gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc
    13681 taaaccgaag attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa
    13741 agcgaattga gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt
    13801 acgtagttta taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt
    13861 gcagactaac tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc
    13921 gacaatcata tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc
    13981 tcttggcaag aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc
    14041 tctaaagtcg acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac
    14101 ttcgttcttt tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa
    14161 tgcaactaat tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc
    14221 cttcgactaa atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta
    14281 aagcggtcac caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata
    14341 atgtgttcaa accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa
    14401 ataaataatc attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa
    14461 tagccaatgg tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg
    14521 ctgcttcttc aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag
    14581 taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta
    14641 gaccggaaat gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg
    14701 tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg
    14761 ccccgctgat cccaatcact actgcagtta cggaggctat caaggagatc actaaactat
    14821 ttttgaaata catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa
    14881 aagtccattc tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga
    14941 atgatgtcat cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg
    15001 tcgcgtaaaa taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg
    15061 tgagagcaag acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt
    15121 tccaagatca ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt
    15181 attcgtcagt agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat
    15241 cacagttgct agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc
    15301 atttgcaccg tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc
    15361 caaaaatgaa ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa
    15421 gaagctagca actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc
    15481 tactaacaga tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat
    15541 caaagaaata gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa
    15601 tctgaacttg cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac
    15661 aactaagaat gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa
    15721 aatttcaaga taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat
    15781 atcgaaaaaa ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa
    15841 aagcatgctt ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg
    15901 attcattgaa acctccccct gataatctgc caatagctat ctactttttt cgaacaccat
    15961 tggcagaact tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc
    16021 ttcgtttccg acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc
    16081 tcccattttc gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc
    16141 actagcaatc tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc
    16201 atccagttcg actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca
    16261 cttttccatg tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg
    16321 tttctcgtct agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc
    16381 tttttgtgtt gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg
    16441 caaaatgtaa gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat
    16501 ttctgtcaca tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa
    16561 tgcttctctc atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc
    16621 tccatttgac aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg
    16681 catagcaatt ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat
    16741 atcatcccaa ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca
    16801 aataccttga acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg
    16861 aacaaggttt ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg
    16921 gactgccgtc tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac
    16981 ttccattact tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc
    17041 tgattcttct accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc
    17101 aaccagttca ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat
    17161 cgttgtctgt tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc
    17221 agtagctagc agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta
    17281 ttattttcag ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat
    17341 ggataatttg tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg
    17401 attgattgga aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac
    17461 tgataaaaga atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt
    17521 ctttttttcg aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat
    17581 ctgtctgcaa ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat
    17641 tctctcctgt tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat
    17701 aaattgccgg cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc
    17761 ttctccattc cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct
    17821 catatggact ggtcgttcca tctgggtttt tcttgtagtt tactcgggta ccctctttct
    17881 tcagctgttc cactaattca aggatcgttt tttccggcag aatacctcgt accggattca
    17941 atccgatacc gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat
    18001 cgatggtttt taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg
    18061 tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat
    18121 aactgatatt atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg
    18181 cttcatcagc gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac
    18241 aagaagttcc tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc
    18301 ctttttctaa gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa
    18361 aattcaagtc tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt
    18421 tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg
    18481 tatccagtgg caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct
    18541 gaaaccaagt actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt
    18601 cgcttctcat ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat
    18661 ttacaacgga gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc
    18721 taaatgtatc tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct
    18781 gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca
    18841 gtttgcctgc ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg
    18901 cggttttctc agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg
    18961 gattcccctt tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt
    19021 actgtcaata gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct
    19081 tatgtatcaa taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta
    19141 ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct
    19201 aaaacgctcc caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga
    19261 aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct
    19321 ctgataaagc aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca
    19381 aaattgcaag aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta
    19441 cttctcatac gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg
    19501 tttctgaaca aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc
    19561 tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct
    19621 ctacgatgga tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa
    19681 tcggaaaagt cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg
    19741 atagttacga tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc
    19801 atgctccgac agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat
    19861 ttgatcacca cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag
    19921 atagcatggc agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt
    19981 ctaccgatgc attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga
    20041 tacctgatga tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc
    20101 cacagttgac tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt
    20161 tattccaact aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga
    20221 ttactctcac agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga
    20281 aacactgagg ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc
    20341 ctcccggtat tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac
    20401 tataatgttt gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt
    20461 tgtgtataag tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt
    20521 tatttgggga tacttatctc taaaacataa atagaataac agacatgtct actggttttg
    20581 ttttattcga ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct
    20641 tcccatcaat ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc
    20701 tcttggaaga tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct
    20761 ttcccacttt tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac
    20821 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
    20881 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
    20941 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
    21001 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
    21061 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
    21121 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
    21181 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
    21241 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
    21301 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
    21361 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
    21421 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
    21481 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
    21541 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
    21601 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
    21661 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
    21721 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
    21781 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
    21841 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
    21901 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
    21961 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
    22021 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
    22081 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
    22141 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
    22201 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
    22261 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
    22321 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
    22381 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
    22441 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
    22501 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
    22561 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
    22621 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
    22681 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
    22741 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
    22801 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
    22861 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
    22921 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
    22981 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta
    23041 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
    23101 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
    23161 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
    23221 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
    23281 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
    23341 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
    23401 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
    23461 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
    23521 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
    23581 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
    23641 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
    23701 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
    23761 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
    23821 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
    23881 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
    23941 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
    24001 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
    24061 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
    24121 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
    24181 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
    24241 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
    24301 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
    24361 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
    24421 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
    24481 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
    24541 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
    24601 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
    24661 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
    24721 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
    24781 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
    24841 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
    24901 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
    24961 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
    25021 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
    25081 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
    25141 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
    25201 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
    25261 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
    25321 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
    25381 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
    25441 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
    25501 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
    25561 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
    25621 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
    25681 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
    25741 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
    25801 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
    25861 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
    25921 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
    25981 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
    26041 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
    26101 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
    26161 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
    26221 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
    26281 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
    26341 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
    26401 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
    26461 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
    26521 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
    26581 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
    26641 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
    26701 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
    26761 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
    26821 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
    26881 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
    26941 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
    27001 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
    27061 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
    27121 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
    27181 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
    27241 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
    27301 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
    27361 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
    27421 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
    27481 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
    27541 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
    27601 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
    27661 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
    27721 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
    27781 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
    27841 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
    27901 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
    27961 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
    28021 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
    28081 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
    28141 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
    28201 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
    28261 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
    28321 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
    28381 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
    28441 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
    28501 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
    28561 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
    28621 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
    28681 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
    28741 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
    28801 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
    28861 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
    28921 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
    28981 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
    29041 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
    29101 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
    29161 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
    29221 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
    29281 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
    29341 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
    29401 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
    29461 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
    29521 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
    29581 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
    29641 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
    29701 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc
    29761 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
    29821 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
    29881 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
    29941 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
    30001 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
    30061 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
    30121 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
    30181 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
    30241 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
    30301 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
    30361 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
    30421 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
    30481 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
    30541 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
    30601 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
    30661 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
    30721 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
    30781 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    30841 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    30901 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    30961 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    31021 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    31081 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    31141 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    31201 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    31261 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    31321 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    31381 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    31441 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    31501 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    31561 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    31621 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    31681 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    31741 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    31801 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    31861 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    31921 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    31981 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    32041 tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt
    32101 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    32161 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    32221 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    32281 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    32341 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    32401 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    32461 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    32521 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    32581 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    32641 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    32701 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    32761 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    32821 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    32881 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    32941 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    33001 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    33061 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    33121 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    33181 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    33241 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    33301 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    33361 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    33421 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    33481 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    33541 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    33601 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    33661 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    33721 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    33781 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    33841 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    33901 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    33961 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    34021 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    34081 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    34141 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    34201 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    34261 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    34321 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    34381 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    34441 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    34501 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    34561 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    34621 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    34681 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    34741 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    34801 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    34861 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    34921 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt
    34981 gatagcacgg atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtgat
    35041 agcacgg
//
LOCUS       DAJSVP010000018        34791 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSVP010000018 DAJSVP010000000
VERSION     DAJSVP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.018"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..267)
                     /locus_tag="ON449_000956"
     CDS             complement(37..267)
                     /locus_tag="ON449_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3933562.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(353..1240)
                     /locus_tag="ON449_000957"
     CDS             complement(353..1240)
                     /locus_tag="ON449_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3933563.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(1329..2552)
                     /locus_tag="ON449_000958"
     CDS             complement(1329..2552)
                     /locus_tag="ON449_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3933564.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(2569..3615)
                     /locus_tag="ON449_000959"
     CDS             complement(2569..3615)
                     /locus_tag="ON449_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3933565.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(3779..5506)
                     /gene="ptsP"
                     /locus_tag="ON449_000960"
     CDS             complement(3779..5506)
                     /gene="ptsP"
                     /locus_tag="ON449_000960"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3933566.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(5506..5772)
                     /locus_tag="ON449_000961"
     CDS             complement(5506..5772)
                     /locus_tag="ON449_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3933567.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6251..8485
                     /locus_tag="ON449_000962"
     CDS             6251..8485
                     /locus_tag="ON449_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933568.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8633..8953
                     /locus_tag="ON449_000963"
     CDS             8633..8953
                     /locus_tag="ON449_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3933569.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(9158..9381)
                     /locus_tag="ON449_000964"
                     /pseudo
     CDS             complement(9158..9381)
                     /locus_tag="ON449_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(9579..11159)
                     /locus_tag="ON449_000965"
     CDS             complement(9579..11159)
                     /locus_tag="ON449_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3933570.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(11560..12936)
                     /locus_tag="ON449_000966"
     CDS             complement(11560..12936)
                     /locus_tag="ON449_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3933571.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(13064..14182)
                     /locus_tag="ON449_000967"
     CDS             complement(13064..14182)
                     /locus_tag="ON449_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3933572.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            14324..14758
                     /locus_tag="ON449_000968"
     CDS             14324..14758
                     /locus_tag="ON449_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933573.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(14836..15369)
                     /locus_tag="ON449_000969"
     CDS             complement(14836..15369)
                     /locus_tag="ON449_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3933574.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(15463..16641)
                     /gene="mutY"
                     /locus_tag="ON449_000970"
     CDS             complement(15463..16641)
                     /gene="mutY"
                     /locus_tag="ON449_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3933575.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(16634..17431)
                     /gene="recX"
                     /locus_tag="ON449_000971"
     CDS             complement(16634..17431)
                     /gene="recX"
                     /locus_tag="ON449_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3933576.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            17538..18920
                     /gene="rlmD"
                     /locus_tag="ON449_000972"
     CDS             17538..18920
                     /gene="rlmD"
                     /locus_tag="ON449_000972"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3933577.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(19094..19672)
                     /locus_tag="ON449_000973"
     CDS             complement(19094..19672)
                     /locus_tag="ON449_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3933578.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            19994..20464
                     /locus_tag="ON449_000974"
     CDS             19994..20464
                     /locus_tag="ON449_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3933579.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(20572..21798)
                     /locus_tag="ON449_000975"
     CDS             complement(20572..21798)
                     /locus_tag="ON449_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3933580.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(22552..22752)
                     /locus_tag="ON449_000976"
     CDS             complement(22552..22752)
                     /locus_tag="ON449_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3933581.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(22886..23494)
                     /locus_tag="ON449_000977"
     CDS             complement(22886..23494)
                     /locus_tag="ON449_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3933582.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(23579..24049)
                     /locus_tag="ON449_000978"
     CDS             complement(23579..24049)
                     /locus_tag="ON449_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3933583.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(24097..25011)
                     /locus_tag="ON449_000979"
     CDS             complement(24097..25011)
                     /locus_tag="ON449_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3933584.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            25157..25960
                     /locus_tag="ON449_000980"
     CDS             25157..25960
                     /locus_tag="ON449_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3933585.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            26138..27085
                     /locus_tag="ON449_000981"
     CDS             26138..27085
                     /locus_tag="ON449_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3933586.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(27153..27446)
                     /locus_tag="ON449_000982"
     CDS             complement(27153..27446)
                     /locus_tag="ON449_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3933587.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(27474..28139)
                     /locus_tag="ON449_000983"
     CDS             complement(27474..28139)
                     /locus_tag="ON449_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3933588.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(28279..28911)
                     /locus_tag="ON449_000984"
     CDS             complement(28279..28911)
                     /locus_tag="ON449_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3933589.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(28918..29985)
                     /locus_tag="ON449_000985"
     CDS             complement(28918..29985)
                     /locus_tag="ON449_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3933590.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(29982..30713)
                     /locus_tag="ON449_000986"
     CDS             complement(29982..30713)
                     /locus_tag="ON449_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3933591.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(30882..31361)
                     /gene="greA"
                     /locus_tag="ON449_000987"
     CDS             complement(30882..31361)
                     /gene="greA"
                     /locus_tag="ON449_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3933592.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(31497..32672)
                     /gene="mltG"
                     /locus_tag="ON449_000988"
     CDS             complement(31497..32672)
                     /gene="mltG"
                     /locus_tag="ON449_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3933593.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            32876..34045
                     /locus_tag="ON449_000989"
     CDS             32876..34045
                     /locus_tag="ON449_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3933594.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(34312..>34791)
                     /locus_tag="ON449_000990"
     CDS             complement(34312..>34791)
                     /locus_tag="ON449_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933595.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        10436 a         7018 c         5731 g        11606 t
ORIGIN      
        1 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
       61 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
      121 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
      181 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
      241 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
      301 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
      361 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
      421 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
      481 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
      541 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
      601 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
      661 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
      721 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
      781 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
      841 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
      901 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtatttgtag
      961 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
     1021 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
     1081 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
     1141 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
     1201 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
     1261 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
     1321 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
     1381 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
     1441 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
     1501 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
     1561 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
     1621 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
     1681 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
     1741 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
     1801 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
     1861 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
     1921 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
     1981 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
     2041 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
     2101 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
     2161 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
     2221 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
     2281 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
     2341 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
     2401 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
     2461 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
     2521 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
     2581 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
     2641 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
     2701 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
     2761 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
     2821 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
     2881 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
     2941 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
     3001 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
     3061 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
     3121 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
     3181 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
     3241 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
     3301 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
     3361 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
     3421 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
     3481 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
     3541 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
     3601 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
     3661 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
     3721 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
     3781 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
     3841 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
     3901 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
     3961 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
     4021 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
     4081 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
     4141 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
     4201 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
     4261 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
     4321 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
     4381 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
     4441 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
     4501 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
     4561 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
     4621 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
     4681 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
     4741 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
     4801 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
     4861 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
     4921 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
     4981 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
     5041 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
     5101 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
     5161 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
     5221 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
     5281 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
     5341 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
     5401 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
     5461 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
     5521 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
     5581 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
     5641 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
     5701 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
     5761 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
     5821 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
     5881 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
     5941 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
     6001 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
     6061 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
     6121 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
     6181 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
     6241 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
     6301 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
     6361 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
     6421 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
     6481 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
     6541 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
     6601 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
     6661 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
     6721 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
     6781 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
     6841 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
     6901 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
     6961 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
     7021 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
     7081 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
     7141 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
     7201 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
     7261 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
     7321 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
     7381 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
     7441 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
     7501 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
     7561 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
     7621 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
     7681 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
     7741 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
     7801 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
     7861 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
     7921 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
     7981 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
     8041 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
     8101 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
     8161 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
     8221 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
     8281 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
     8341 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
     8401 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
     8461 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
     8521 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
     8581 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
     8641 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
     8701 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
     8761 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
     8821 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
     8881 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
     8941 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
     9001 acacttttgt ctcacgctct aaacacgtat aaacggcgaa agcagaagca actccttcgg
     9061 aaataagctg aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
     9121 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
     9181 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
     9241 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
     9301 ttacatctaa taatcgacat aatcgtaaca tctcttcttt ttctgttttt ttctttcacg
     9361 tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa
     9421 taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa aaagagccgt
     9481 cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc
     9541 ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt
     9601 acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac
     9661 ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa
     9721 tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg
     9781 ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca
     9841 atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat
     9901 tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata
     9961 aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac
    10021 aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact
    10081 gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt
    10141 tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt
    10201 cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg
    10261 aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca
    10321 taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc
    10381 aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat
    10441 gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt
    10501 tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca
    10561 tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct
    10621 tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc
    10681 tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata
    10741 aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct
    10801 tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga
    10861 taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct
    10921 tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc
    10981 atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg
    11041 atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg
    11101 gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata
    11161 acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat
    11221 ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt
    11281 tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat
    11341 acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa
    11401 cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac
    11461 caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg
    11521 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
    11581 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
    11641 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
    11701 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
    11761 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
    11821 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
    11881 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
    11941 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
    12001 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
    12061 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc
    12121 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
    12181 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
    12241 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
    12301 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
    12361 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
    12421 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
    12481 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
    12541 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
    12601 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
    12661 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
    12721 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
    12781 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
    12841 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
    12901 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
    12961 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
    13021 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
    13081 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
    13141 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
    13201 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
    13261 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
    13321 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
    13381 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
    13441 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
    13501 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
    13561 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
    13621 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
    13681 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
    13741 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
    13801 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
    13861 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
    13921 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
    13981 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
    14041 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
    14101 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
    14161 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
    14221 ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg
    14281 ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac
    14341 aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt
    14401 agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca
    14461 cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga
    14521 aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac accaagggtt
    14581 aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat
    14641 tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta
    14701 cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa
    14761 atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac
    14821 tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat
    14881 atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt
    14941 ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata
    15001 ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata
    15061 gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata
    15121 ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata
    15181 aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg
    15241 atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt
    15301 tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg
    15361 aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta
    15421 caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt
    15481 gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga
    15541 atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc
    15601 tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg
    15661 agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag
    15721 attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca
    15781 ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat
    15841 attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg
    15901 aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa
    15961 actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca
    16021 ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc
    16081 acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga
    16141 agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc
    16201 gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg
    16261 tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc
    16321 tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc
    16381 ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa
    16441 ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat
    16501 gatctcagag atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt
    16561 ttcttgttca taccattgaa taaactgatc ttgaaattct tttgtttctt tatctgtcca
    16621 tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat
    16681 ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt
    16741 caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta
    16801 tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa
    16861 agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt
    16921 gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg
    16981 tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct
    17041 gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc
    17101 catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat
    17161 cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact
    17221 gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga
    17281 tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata
    17341 catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt
    17401 tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta
    17461 tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa
    17521 gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga
    17581 aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat
    17641 ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc
    17701 gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac
    17761 catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc
    17821 aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct
    17881 ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca
    17941 aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg
    18001 aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac
    18061 aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg
    18121 aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg
    18181 agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc
    18241 gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa
    18301 aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag
    18361 aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc
    18421 agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa
    18481 cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca
    18541 aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg
    18601 aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta
    18661 aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt
    18721 tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct
    18781 cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg
    18841 aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga
    18901 tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa
    18961 taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa
    19021 gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag
    19081 ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat
    19141 cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga
    19201 tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt
    19261 caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat
    19321 cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat
    19381 taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga
    19441 ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg
    19501 gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt
    19561 atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga
    19621 acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac
    19681 ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt
    19741 ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag
    19801 tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa
    19861 catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt
    19921 acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa
    19981 aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat
    20041 tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc
    20101 tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact
    20161 gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg
    20221 aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct
    20281 attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt
    20341 cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctgacaatgt ggctcatgat
    20401 tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa
    20461 ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta
    20521 gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt tctagtaccc
    20581 acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt cctgtccagc
    20641 gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg tatcttctgt
    20701 ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg aataggaaga
    20761 ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc catcttccga
    20821 atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg tatcgcttgt
    20881 ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat tttttcctaa
    20941 aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt cttgtttgat
    21001 tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc aatctaagtc
    21061 cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta cgccatctaa
    21121 acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat
    21181 atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt ctccattcaa
    21241 tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt tattattcac
    21301 tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga tcgttacata
    21361 atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt cgacaccacc
    21421 aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat tcgtataaat
    21481 atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg ttactaccat
    21541 catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta aacctaatag
    21601 caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt tggaggatat
    21661 tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca taaatctgat
    21721 tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg ttaatagaac
    21781 tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat tgtctcatat
    21841 aactgttcaa tcagcaaatt cactttttaa tcataagaaa actttaaaaa acatttttgt
    21901 gtaacgatta ttatttttta aaatttctca aaattcttaa ctatcatacc ataccgtgaa
    21961 aaagatcgaa agaccgaata cacgcagttc gcaatcgttt tatctttttt tatgtatttt
    22021 ttccagaaaa agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac
    22081 atacctttcc catcttttta acagtctttg cctaccgttc agattatcat tcgatttcac
    22141 gacaatttct tcctatgact ggtccttttg ttaaacccat cttctattcg ttttatttcc
    22201 agctcactgc atggctctta aaatgactcg aactagtgat tgcattacca tcatctttta
    22261 atggatagat atgctcctct tcaaagtaaa acgattttct tttaaagtaa tagaattgac
    22321 atggtaatga gtatgtatac tagtggtttc acaaatggtc ttttattcaa catccaaaaa
    22381 ctgaaatttc tttaaaatcc attactaacg aagaaaatgt tccttgcttg ataattagtt
    22441 aggacatgtc aagtgaacaa atgcatttac ttagccaaaa aatctaaaaa cttcataaat
    22501 agacaaaaag acacgactct ctaagaagtc gtgtctttat tcaacaaatt attataattt
    22561 agtcacttca gcagcttgag gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg
    22621 accttcttct aggctcttga agccgtctcc ttgaattgct gagaaatgaa caaatacgtc
    22681 atctccgccg tctactgtaa taaacccaaa tcctttttca ttgttgaacc atttaaccgt
    22741 tccgtgttcc ataaatgtat atcctccaaa aaagttcttg caagtaacct tgcaagaaca
    22801 ttatacccaa tttagaaaac gctagcaagc tttcgctctt ttattttcca aattattttt
    22861 aagactgata tgacggagga aatggttatt ttgctgcttc gaaacgtttg tttacttcat
    22921 cccaattaat aacattccag aatgctgcaa tataatctgg tcggacattt ttatatttca
    22981 aataataagc atgttcccaa acgtccaaac ctagaattgg tgtctttcct tccatcaatg
    23041 gagaatcttg atttgcagta gaggtaatag ctaattttcc attttccatt acaagccaag
    23101 cccatccaga accaaatcgt ccagcggctg cttttttgaa ttcttcttta aaagaagaaa
    23161 aatcaccaaa agcttcatta atcgcttctt ttatttctcc tgtaggttcg ccaccagcat
    23221 ttggtgccat gatttcccag aaaaatgaat ggttagcatg tccgccacca ttgttacgta
    23281 cagctgtctt gatatctgtt ggaatagcgt ccatatcaga taataattct tctattgttt
    23341 tttcgcctaa ttctgggtat ttctcaattg ctgaatttaa attcgtcaca taagtattgt
    23401 gatgcttatc atgatgcaga tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat
    23461 atgcataagg tagatctggt aatgtgtaag tcataaaaaa attcctcctc aatgttttct
    23521 taataatgtc tacattacaa agagtaacaa agagaaggaa attcttcaat tgttatgcct
    23581 agtcgtcttt ttcactttga cctacttctt cttcaaaaac ctcagtttct ttaaaaataa
    23641 acaacttgct gaaaatatag ttcgcaagga taacaataat ttgagtgatg attttcgcca
    23701 catactcatt gctgttcata acattgatca acaataccat tgcccccatg tccaatccgt
    23761 aagaaaggat acggtagaag aagaacttac cgcattcaat aagtgcatgt gtaaaattcg
    23821 gtgattttga atggaatacc caaagtttgt tcgttacaaa agcaaacaat acagaacaaa
    23881 accacgaaat agaattggat aaggccatac ttaaatcaaa atattggcgt gcgagagcaa
    23941 aactaacaaa atttacaatc gtagccaaac caccaaaaaa gagatagact agtatctccc
    24001 aatatccttt tgcttctaaa tatcctttta actgtcgata ctgcttcatg agccaaccta
    24061 ccttattaaa aattcttatt tatcatactc tatatttcaa taactttcat ctttatttaa
    24121 aaaaattaat tttttttgat cctttgcctt tgtctctgct tggatcgtca catgtgtaat
    24181 accatatttt aattttaata aagaaattat acgaggatat atcttttcaa tctcgcttag
    24241 tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc tatactgcca
    24301 aatatggaca tgatgaacat cctttacccc atcgattgct tcaatttctt gttccatttt
    24361 ttcatagtcg atagcaggtg cactttgtat caaaatagcg gcagcttttt tgatcaccat
    24421 tatcgcttct atcaatatat atatcgaaat gatcagtgta ataactggat cgatccagta
    24481 gatatcgaaa aaacggatca aaattgctcc gataatcaca cttaccgaag acagtgtatc
    24541 acttaacaaa tgcaaataac tagattttat attcaaactt tcatctgctt cttgtcgtaa
    24601 taataatact gagaacaaat tcgcgagcaa tccaataact gctactgtta gcatcaaatg
    24661 actatttatc ttttctggtg aagtgaaccg cttgaatgct tcaacggtta aaaacaagga
    24721 taatatgatc aatatgaatg agttgatgaa agcactcaat atttctaaac gctggtagcc
    24781 atacgtacgt tttttatttg cttcttttcg ggccattttt tgtgccccgt aagaaaaaat
    24841 aattgccaat gtatcactaa ggttatggaa agcatctgaa acaagagaca aactccctga
    24901 cataatccct ccaataaatt ctgataaagt gatcaccgca ttcaaaacca ctactccaaa
    24961 tagttttttt gatgatctct gtggctgtgt aggcactatt ccttcctcca ttttatttat
    25021 attggatagt ctttagacaa gaataacatt tttttgatat ttcaccaatc attcagctgt
    25081 ttccttaaag cacttgtatt tcacttgata cttcgctaga attgcaagtg atgacactac
    25141 tagatgagga gtacttatga caacaaaaca attaattatc agttccttta tacgattcaa
    25201 agacctaaag gaggcgagaa atgtcccatt ttggaaagcc attgtctatc ttttagtttt
    25261 gagtataatt atggctttgc caatctcttt tcaagttttt caagtcttaa acaacattca
    25321 attggatggg caaaagattg ctactcgcat tcctgatttt acaatccaaa acgggcagct
    25381 tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac tccattatct tcacatttga
    25441 tccagaggga aaacgaacag aacaggacat ctctactgat ttgatgggga attttgttag
    25501 tgtcgggatg ttaaaaaaca aacttatcat agctcttcct aatacaggca cgacttctgc
    25561 tttgttgaac aataaccaat tagaacttcc ctacaccaat gaatctttaa aaaacttaac
    25621 aggtaagcag ctccgttctt ttttaagtga agcatctata ccactttgga taaaagtcct
    25681 gacatttata ttttctattt atcctagttt tctgaatctg ctgatcactt tgctatttac
    25741 caacattgct gcttttctct atgcacgctt tcgactaaca aaagctactt ttcttgattg
    25801 tttaaaaaca ttgatctatt caatttcatt gcctactata attgcaacaa tcttgatggt
    25861 ctttcttcct tcttttgaca ctagcgcctt cattgctttt gcaggaatct ttatttttgc
    25921 tcaagctgtc aaaggatggg caaaaatatc gatcaattaa gaaataaaga caactcgtta
    25981 tttctacttc aatagaccct tttagaggtt gtgacatttt tttgtcacag cccctttttt
    26041 atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg
    26101 cgctacactt agagtaaaga aaggcgggaa attaactatg tcagaaagaa tcatttatgc
    26161 agatcgtcca tttcgtgatg aatttatcga agaaatccat actatagcgc ctggttatat
    26221 ctttaaaaca gaattacaac cagaggatct acaaaatgta gagatcagtt tgggctggaa
    26281 ctcagactac caagaaaaac tattagcctc ttctaccttg aaatgggtcc aatctatctc
    26341 agctggtgtt gatcagctac cccttgagac tttttcaaag aaacatattt tactttcaaa
    26401 tggtagtggc atccacagtg aatcgattac ggagcatgtc ttaggaatca tccttgggta
    26461 ttctcgtggt ctttttcaag cacaaaaggc acagagtgac atgaaatggt taggttcatc
    26521 ggtccattac caatctatcc aagggaaaaa aatactaatc attggaactg gccatattgg
    26581 aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa tcaatacatc
    26641 cggtcatcca gcagaaggaa tggaaaagac tttttcttta aatcaactaa aggaagttct
    26701 tcctgaagta gatttcgtag tcaatatctt acctctaacg gaggaaacaa ctgggctctt
    26761 tgatcagcat atgtttgaac actttgatcc aaaatctgta ttcatcaatg ttggccgtgg
    26821 tgcttctgtt aagacacaag atctggtgca ggctttaaat aaccagcaac tttcctttgc
    26881 agcattagac gtttttgagg aagagcctct tccacaagat catcctcttt ggaaaatgga
    26941 caatgtactg attacttccc acattgcagg tttgacacct gattttcaaa agaaattaat
    27001 ggctattttt ttaaccaatc tgaaaagcta ctttgccacc catgagcttc aaaccaatca
    27061 agtaaaattg tctgcaggat attaatagct gaaaacaata ttttcaaaag aaaaaagaga
    27121 aggtgacaaa aattgtgtca ccctctcttt ttttattgtt ttttaaagtg ataggttggc
    27181 tcctccaggt tgggatcata atcaacaagt aaatcatatc ctttaaagaa ccattcatct
    27241 gcttcctcga taaaaaaaag tgtcccatcc atttccactt tctttacagg atgttctggc
    27301 tgttcaactg acattccaat cgaaaaacca tcatgaacct ctgttttgcc gtagactttt
    27361 ccaaaaaaac gtattccgta agtttctggc agatcaattt ctgagtcaaa ccacttttgt
    27421 gcttttggcg ttaccgttaa tttcatccca acaacctact ttctcttttc tttctattga
    27481 ttcatcttat cgtctagaat atccacatcg cctgtctctc caataaccag tagattatca
    27541 ttttcacgaa catattcatc tgctgcagga gaagcgataa ccgtttgatc tactcttcgt
    27601 atggcgacta ctgttaagtc aaaacgttgc ctgaaattta attcagctaa tgttttgttg
    27661 aaaaatttag gattggagac tcggatttct gctaaagaat atttatctga caactctagg
    27721 taatctaaaa tattctttga cactaaatta tgcgcaatcc ttattcccat gtctcgctcg
    27781 ggatggacga cgcgatcagc tccgatcttt tcaagtactc tggcatgata ctcattttgg
    27841 gcttttgcta aaatatttgg tacgcccatt tcctttacca tcagagtaac taagatactg
    27901 gcttgaatat cttctccaat agcgacaatt acatgatcga aatttcttat gcctaatgat
    27961 cgaagtgtca tttcatcctg tgcatttgcc acaacagcat gagtggctat attcatatat
    28021 tcattgaccc gatcttcatt actatcaata gccagtactt cttgaccagc ttcgatcagt
    28081 gttcggcaga tacttccgcc aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc
    28141 gttttcctcc tttagtatct tcttttctca gtataacaaa gcctttacaa aaacaaaagc
    28201 aagcatcatt attgtaaaaa agccaagaaa aataatagtt attttctcag cttttgatta
    28261 tcctctttat catcgttttt atttcaccaa accatgtttg aatgcataga tagcggcttg
    28321 tgtacggtct tccacttcta gcttagccaa tatattggaa acatgtgttt ttactgtttt
    28381 cagtgtaata aacaattcgt ccgctatttc ctgattactt tttccttcag aaatcagcat
    28441 taaaatttca ttttcccgat tcgttagttc ttcatgtaat acaggctcat ttcgccgact
    28501 cattttttcc atcatttttg ttgtcacttc tggttctaat actcgctcgc cacgttgcgt
    28561 tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc
    28621 acctgcttca atggcaggat aaactttttc gtcatcaata aaactggtta cgataatgat
    28681 tttagcttct ggccaatctt ttaagattgc ttttgttgat tcgataccat ccatttcttc
    28741 catgactaag tccattaaaa tgacgtccgg tcgaagtgtc atcgccttct catagccttg
    28801 tcgtccattt tcagcttctc caattacttc aatgtcttct tgaatagata aataagaaga
    28861 gacgcctaag cgcaccattt catggtcatc tactaataaa acttttatca tccttatcta
    28921 tgcctccttc atcaaaggga ccttgatttc tacacttgta ccttgacctt taaaactaat
    28981 gatttttact gttccaccaa taccagctac tcgttctttg atattgttca atccataact
    29041 tcccgttttc gtttcattca tattaaaccc tgttccgtcg tcaataatac gtaacaaaag
    29101 attattgtct attttgtgca aatatacctc taattcattg gctttagcat gtcttaatgt
    29161 atttgataac aattcttgaa cgattcggaa caagtgatcc tcaatggaac tagatagttt
    29221 tacatcttct acatcccatt tcagcgaaat ctggatttta ttttgcaatt ccttcaataa
    29281 ctgctcgata ccttgcttta aacttttctc ttctaaattg actggacgta gatgaagaag
    29341 cagcgcacgc atctcagact gggaagcgtt gatgatctct gctaccattt tcaactgctt
    29401 gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc gtcaatgccg acatcatcat
    29461 cattgctgca aacaattgtt gcgaaactga atcatgcagc tcacgggcta atcgatgtct
    29521 ttctaattct aaaatttctt ctttggtttg tccatctaca tattggggac ggctcgttac
    29581 aatttgtaat tcactagata cttcaatcat tttttctttt atcttagtga tttccttatc
    29641 aatatctttg atgtataaat cgtccgatgc acttgggatc ggaagaagca acaatttgga
    29701 ttcataattg ccagaagaaa gtgcacgcag cttttcttcg attttcccgt attttgtctt
    29761 ttgaataagt gataataata gaaagacaat taatcctaca cctaaggaaa tggctaacaa
    29821 atggaagata agcggaacat ataataaacg tgcacgcagt aattctcccc accaatgact
    29881 ttgattgctg gcataaaaat aagtgaaaag tgaaaataga ataataagga aagccgcaat
    29941 ccccgaataa acagctagca tcgctctgga tatttttccc atcatattcg tatcacctcg
    30001 acatctccaa tcaaggtatt agtaataatc ttcaatctac gagggttttc atcataatca
    30061 ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt gctcttcttc aaacaagaca
    30121 tttcctacta atgtcgcatg ctctaatcgg atggctacgc ccaaaggtac taagatccgt
    30181 gttctgccta tcccttttct tacaatcaca atattatcat cttttggcaa aagcgtattg
    30241 cccaaatcta tgatagtatc accagaaatg acggcgatgt tgatatcgtc ccattcatag
    30301 acgtcgtttc caatacgttg gttaccgaat aattgctgtc ttttccgctc attattatgt
    30361 gtttttaatt gctctgtttg gatcatcatg atctgtttct ttcgccaaaa agcatttttg
    30421 gttaaatcta tccctgaaat ctctacacct ttcagaccaa taaataaaac ggcaaataca
    30481 gccatcatcc ataaagcagg actgttgact aagctaaaaa aaatgaccaa acttcctaat
    30541 atcaattgaa aattttgaaa tttagtacga ggatgtttgc gcatagctag atatatattg
    30601 aaaataccga atatcactaa aagcaaaagt ccagtattgt ttacgatttg ccaaacagca
    30661 aataacagca gtagtgcttc aactaccaca aaaaaacgcc aagaactatt catcgctctt
    30721 tctccctcct gacacattct catttcatca tacctatttt tttaactgtt ggctatagga
    30781 ctttagatgt aagatcgttt gttgaaatct aaaatacaca aagagaaaaa gatctagagt
    30841 gtatcatcag taagatgaac tctctagatc tttcagtcag actacgcttg ttctactttg
    30901 acgattttaa ctaacatatc gccacctgga gtagcaatag ttacttcatc atttttacgt
    30961 ttgccaatca atgcttgcgc aataggagaa tcattggaaa ttttgccaga aaacggatct
    31021 gcttcagcgc ttccgacaat cgtgtactct tcttcttctc catctggtag ttcgatgaat
    31081 gtaactgttt taccaattga tacctcatct gcatctacac cgtcgttatc gatgatttga
    31141 gcaaaacgaa tcatattttc aattgttgtg attcttcctt caacaaatgc ttgttcatct
    31201 ttcgctgatt catattcaga gttttcggaa agatctccga aaccgcgggc aattttgatt
    31261 cgttcaatga tttctccgcg tttgactgtt tttagttctt ctaattcttt ttctaatttt
    31321 tctttacctt ctagagtcat tggataaact ttctctacca tgtgtaacgt ctcctttttt
    31381 cgataaaata ccgcaaaagg taatcttttg aggaatatag tccaaaaaag attaccacgc
    31441 ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg tctagagcct gtttttctaa
    31501 gaattattta catatttttc cactaattcc atatgttctt cataagtttt agcgaaataa
    31561 acatttccag tgctcgtatc tgctacgaaa taataatagt cattttctgc tggttctaat
    31621 actgccttga ttgcctcttc gcttggattg tcaaaaggtc caggtccata gccggtattg
    31681 gtatacaaat tatatggtga atctacagct gtatcttcat acgtcactaa ttctttgtgt
    31741 tcacctaatg cgtataaaac agagatatct gactgaagtg gcatatttgc tttgattcgg
    31801 ttaaagaaga cttgtgcaat gtttttacga tcattttctt ttacgccttc tttttcaact
    31861 agagaagcta gagttaaaac ttcttgtaca gtcaaatttt tctgcttgat agctgaataa
    31921 tagttttgca tgaccgtatt cgtcttgttc accatttcaa tcactagatc cttaagcgaa
    31981 gtactcgtgt aataatcata tgtggctggg aataagtaac cttccaaacg gtattttgtg
    32041 ttctcagctt tagatgcact ttccaacagt tctgggaatt tttggtgcaa ctcgttaaag
    32101 aaagtctcat cattcactaa atctaaaaat tctttcttgt ctttaccagt taccttcgct
    32161 acccgttcag caatctgatc gatatcgtac ccttctggaa tggcaatttt cccatctgca
    32221 acttttgttg gctcagaagt accgccttct tgcaactgtt tgccaatctc gtcaagagtc
    32281 atattaggcg ctaactgata atagccagct tgaaagccag ttaaattttt aaattttgta
    32341 taataattga aaacaatccc gcttttaata atattgtcct tctccaaaat ctcaccaatc
    32401 tgcttgttag aagagccact cgggatctcg acttggacta attggtcatc tgatttatct
    32461 aaaggcttca acccgctatc tacataacga tacacggtaa atccaagaaa tccgccaata
    32521 atcaataatg taagagcaat cactaaaatg atcttcctca caatcttatc ttctttttta
    32581 cgcagatttc tccgcttgtc gattatatct gctgtagctg ccttactggc ttctttttta
    32641 tctttacttg ggtgttgttt gtttcctgac aaaacaactc ctccttacta tcaagttcgt
    32701 aactttattc attatacatg atatttgaat tggcgtgaat gattttattt aaatattcta
    32761 tcaaaaatgt aagtagtaaa tccattacta taagattaaa ataaatgtta tcaagataca
    32821 agctatttta gctttattct ttctgtaaaa aagaaaggag actaaataaa ctattatgaa
    32881 aaaaggaaaa catgctaaaa aaagaaaata ttcattttta atgatcgttg tcgtgttgtt
    32941 tagccttagt gcgttctcta caggaactat tcttggatgg caattttaca ataatgaaca
    33001 ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga
    33061 acaacagctc gttgaaaaaa tcgatcaact attaagaaaa aatgattata ttggctcaat
    33121 ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc tatggctatg cgaataaact
    33181 cacgcaacaa ccaaatgatc cttctcttta ttatcagatt ggttcggttc aaaaagcatt
    33241 gactactctt ttgattttaa aacaagtcga gcgtgggcac ctttctttag atactaagct
    33301 ggctcaattc tatccacaaa ttgctggcag ccagcaaatt actatccgtg acttgctgta
    33361 tatgcgctca ggcttgcacc gaactgcttc tccaaccata ccgatgactg atgaagaagt
    33421 cgttcaattt gcgattcagc acttaaaatt tacagattat cagacctctc attatgattc
    33481 attgaacttt accatattga ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa
    33541 attattgacc gatgatatca tcgaaccttt aggattacag catacaaaat tttatgacca
    33601 agtcaaaaat tcatccaatc atgcactatc ctatcaaatg tctaaagagg atgattactt
    33661 taaaccactg acagaatctg aaacagagat ccataacgaa ttagggactg gtaatatcag
    33721 tatgtccgtc tatgatttaa atacgctgtt tacacaagtt ttatctggaa aactagtttc
    33781 caaagactta ctcttctcat tatggcgtga aaatgcgaat ggacatccat atagtggtgg
    33841 ggtatatagc ggagataatt atatcctgtc gcaaggaaat atcgactctt ttgatacatc
    33901 tgctatattt agcaaagatt cacaggatgc tatcatcatg gaaagcaata taaaagctga
    33961 caaaactatc aaactgccta ctgctgatct aagaaaccaa atctatgacc tgatggaagg
    34021 aactactaag ttggcaaata gctgatatga tgaaaagata gcaaatacta cttctttgaa
    34081 tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa agagtgtggg acaaatctaa
    34141 aaatcaattt ttgctacaga ctttaattcc aaataaacag aacagaaaga ggtgttcttc
    34201 tttctgttct gtccaaatta ttctaagatt tctctttacc aacattattt gacaaagggc
    34261 ctattaaatc taaaatagta tctttagatt gctaaaatat gtgctagcaa atcattcatg
    34321 gcttgttctt atttttaatt ttatgcaata aaaaagaaga acaattagcg tgattataga
    34381 aataaataat gagattttag aaaaaaatgt gtatgaatac ctgattgtta ctttatttat
    34441 tccactttca ctttttactg taacaaaagg acctccttgt tttagtactg tagcattttt
    34501 tgaattaaca gtaaccgtta atcctttata ccggaataca ggtaaaacta ttgttttgcc
    34561 ttttgaagat ttaaacgtaa atgtaatttc atctttattt ggagaaaagc tactataaat
    34621 aacttgacca tcttctgtaa cttgatggtt tttcaaaagc aattgatctt ttttatagcc
    34681 tataggtaca taatcaaaat tctcaaattc ttcaagttta ttttccattt gttcattaga
    34741 ataagttcct tgaaaattca tttctttcac catgttaact actgatgaat a
//
LOCUS       DAJSVP010000019        34563 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSVP010000019 DAJSVP010000000
VERSION     DAJSVP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.019"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            212..604
                     /locus_tag="ON449_000991"
     CDS             212..604
                     /locus_tag="ON449_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3933596.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ON449_000992"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ON449_000992"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3933597.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="ON449_000993"
     CDS             3165..4775
                     /locus_tag="ON449_000993"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3933598.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="ON449_000994"
     CDS             5067..5936
                     /locus_tag="ON449_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3933599.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ON449_000995"
     CDS             6101..7372
                     /locus_tag="ON449_000995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3933600.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ON449_000996"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ON449_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3933601.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ON449_000997"
     CDS             8763..9026
                     /locus_tag="ON449_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3933602.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ON449_000998"
     CDS             9471..9920
                     /locus_tag="ON449_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3933603.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ON449_000999"
     CDS             9977..11395
                     /locus_tag="ON449_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3933604.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ON449_001000"
     CDS             11395..12255
                     /locus_tag="ON449_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3933605.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ON449_001001"
     CDS             12429..13202
                     /locus_tag="ON449_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3933606.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ON449_001002"
     CDS             13349..13714
                     /locus_tag="ON449_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3933607.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ON449_001003"
     CDS             complement(13862..15070)
                     /locus_tag="ON449_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3933608.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ON449_001004"
     CDS             complement(15089..15367)
                     /locus_tag="ON449_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933609.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="ON449_001005"
     CDS             15521..16216
                     /locus_tag="ON449_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3933610.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="ON449_001006"
     CDS             complement(16658..17455)
                     /locus_tag="ON449_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3933611.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ON449_001007"
     CDS             complement(17452..18636)
                     /locus_tag="ON449_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3933612.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ON449_001008"
     CDS             complement(18661..19908)
                     /locus_tag="ON449_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3933613.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ON449_001009"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ON449_001009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3933614.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ON449_001010"
     CDS             21888..22202
                     /locus_tag="ON449_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3933615.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ON449_001011"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ON449_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3933616.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="ON449_001012"
     CDS             22887..23531
                     /locus_tag="ON449_001012"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3933617.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ON449_001013"
     CDS             23546..23875
                     /locus_tag="ON449_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3933618.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ON449_001014"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ON449_001014"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3933619.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ON449_001015"
     CDS             24863..25687
                     /locus_tag="ON449_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3933620.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ON449_001016"
     CDS             25680..26027
                     /locus_tag="ON449_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3933621.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ON449_001017"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ON449_001017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3933622.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ON449_001018"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ON449_001018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3933623.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON449_001019"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON449_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3933624.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON449_001020"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON449_001020"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3933625.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="ON449_001021"
     CDS             31657..32451
                     /locus_tag="ON449_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933626.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ON449_001022"
     CDS             32441..34483
                     /locus_tag="ON449_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933627.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11113 a         6125 c         7258 g        10067 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 ttt
//
LOCUS       DAJSVP010000020        33402 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSVP010000020 DAJSVP010000000
VERSION     DAJSVP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.020"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            292..1431
                     /locus_tag="ON449_001023"
     CDS             292..1431
                     /locus_tag="ON449_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3933628.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ON449_001024"
     CDS             complement(1498..2073)
                     /locus_tag="ON449_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3933629.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ON449_001025"
     CDS             complement(2078..3052)
                     /locus_tag="ON449_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3933630.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ON449_001026"
     CDS             3335..4303
                     /locus_tag="ON449_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3933631.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ON449_001027"
     CDS             4416..4694
                     /locus_tag="ON449_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3933632.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ON449_001028"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ON449_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3933633.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ON449_001029"
     CDS             5151..5906
                     /locus_tag="ON449_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3933634.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ON449_001030"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ON449_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3933635.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ON449_001031"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ON449_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3933636.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ON449_001032"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ON449_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3933637.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ON449_001033"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ON449_001033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3933638.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ON449_001034"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ON449_001034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3933639.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ON449_001035"
     CDS             10643..11185
                     /locus_tag="ON449_001035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3933640.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="ON449_001036"
     CDS             11208..12764
                     /locus_tag="ON449_001036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3933641.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ON449_001037"
     CDS             12778..13680
                     /locus_tag="ON449_001037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3933642.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ON449_001038"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ON449_001038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3933643.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ON449_001039"
     CDS             15123..15545
                     /locus_tag="ON449_001039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3933644.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="ON449_001040"
     CDS             16014..16244
                     /locus_tag="ON449_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3933645.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="ON449_001041"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="ON449_001041"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3933646.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="ON449_001042"
     CDS             17655..17831
                     /locus_tag="ON449_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3933647.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="ON449_001043"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="ON449_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3933648.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="ON449_001044"
     CDS             18326..18793
                     /locus_tag="ON449_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933649.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="ON449_001045"
     CDS             18878..19762
                     /locus_tag="ON449_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3933650.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="ON449_001046"
     CDS             19876..20112
                     /locus_tag="ON449_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3933651.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="ON449_001047"
     tRNA            20200..20280
                     /locus_tag="ON449_001047"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="ON449_001048"
     tRNA            20287..20358
                     /locus_tag="ON449_001048"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20483..20653
                     /locus_tag="ON449_001049"
     CDS             20483..20653
                     /locus_tag="ON449_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933652.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            20873..21580
                     /locus_tag="ON449_001050"
     CDS             20873..21580
                     /locus_tag="ON449_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3933653.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="ON449_001051"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="ON449_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="ON449_001052"
     CDS             22400..22687
                     /locus_tag="ON449_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933654.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="ON449_001053"
     CDS             22795..23115
                     /locus_tag="ON449_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3933655.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="ON449_001054"
     CDS             23196..24509
                     /locus_tag="ON449_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3933656.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="ON449_001055"
     CDS             24506..25225
                     /locus_tag="ON449_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3933657.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="ON449_001056"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="ON449_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3933658.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="ON449_001057"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="ON449_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3933659.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSIILADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="ON449_001058"
     CDS             join(28786..28857,28859..29887)
                     /gene="prfB"
                     /locus_tag="ON449_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3933660.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="ON449_001059"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="ON449_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3933661.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="ON449_001060"
     CDS             30662..31555
                     /locus_tag="ON449_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933662.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..33155
                     /gene="cls"
                     /locus_tag="ON449_001061"
     CDS             31707..33155
                     /gene="cls"
                     /locus_tag="ON449_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3933663.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        11106 a         5821 c         7245 g         9230 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattat
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag
    32641 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    32701 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    32761 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    32821 tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg
    32881 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    32941 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    33001 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    33061 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    33121 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    33181 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    33241 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    33301 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    33361 agaaactttt aatgagatag ttttttttac aattatgaga at
//
LOCUS       DAJSVP010000021        30347 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSVP010000021 DAJSVP010000000
VERSION     DAJSVP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.021"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..204)
                     /locus_tag="ON449_001062"
     CDS             complement(<1..204)
                     /locus_tag="ON449_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933664.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
     gene            complement(192..1544)
                     /locus_tag="ON449_001063"
                     /pseudo
     CDS             complement(192..1544)
                     /locus_tag="ON449_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1541..2473)
                     /locus_tag="ON449_001064"
     CDS             complement(1541..2473)
                     /locus_tag="ON449_001064"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3933665.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2470..3246)
                     /locus_tag="ON449_001065"
     CDS             complement(2470..3246)
                     /locus_tag="ON449_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3933666.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3300..6485)
                     /gene="carB"
                     /locus_tag="ON449_001066"
     CDS             complement(3300..6485)
                     /gene="carB"
                     /locus_tag="ON449_001066"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3933667.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6490..7566)
                     /gene="carA"
                     /locus_tag="ON449_001067"
     CDS             complement(6490..7566)
                     /gene="carA"
                     /locus_tag="ON449_001067"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3933668.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7571..8851)
                     /locus_tag="ON449_001068"
     CDS             complement(7571..8851)
                     /locus_tag="ON449_001068"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3933669.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8870..9796)
                     /locus_tag="ON449_001069"
     CDS             complement(8870..9796)
                     /locus_tag="ON449_001069"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3933670.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9809..11092)
                     /locus_tag="ON449_001070"
     CDS             complement(9809..11092)
                     /locus_tag="ON449_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3933671.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11106..11645)
                     /gene="pyrR"
                     /locus_tag="ON449_001071"
     CDS             complement(11106..11645)
                     /gene="pyrR"
                     /locus_tag="ON449_001071"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3933672.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11880..12791)
                     /locus_tag="ON449_001072"
     CDS             complement(11880..12791)
                     /locus_tag="ON449_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3933673.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12788..13252)
                     /locus_tag="ON449_001073"
     CDS             complement(12788..13252)
                     /locus_tag="ON449_001073"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3933674.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13257..13955)
                     /locus_tag="ON449_001074"
     CDS             complement(13257..13955)
                     /locus_tag="ON449_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3933675.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13942..14328)
                     /locus_tag="ON449_001075"
     CDS             complement(13942..14328)
                     /locus_tag="ON449_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933676.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14475..16145)
                     /locus_tag="ON449_001076"
     CDS             complement(14475..16145)
                     /locus_tag="ON449_001076"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3933677.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16328..16561
                     /locus_tag="ON449_001077"
     CDS             16328..16561
                     /locus_tag="ON449_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3933678.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16660..17091)
                     /locus_tag="ON449_001078"
     CDS             complement(16660..17091)
                     /locus_tag="ON449_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3933679.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17170..17583
                     /locus_tag="ON449_001079"
     CDS             17170..17583
                     /locus_tag="ON449_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3933680.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17621..18769)
                     /locus_tag="ON449_001080"
     CDS             complement(17621..18769)
                     /locus_tag="ON449_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3933681.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18891..19487)
                     /locus_tag="ON449_001081"
     CDS             complement(18891..19487)
                     /locus_tag="ON449_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3933682.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19687..22329)
                     /gene="alaS"
                     /locus_tag="ON449_001082"
     CDS             complement(19687..22329)
                     /gene="alaS"
                     /locus_tag="ON449_001082"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3933683.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22717..23148
                     /locus_tag="ON449_001083"
     CDS             22717..23148
                     /locus_tag="ON449_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933684.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23193..24542)
                     /locus_tag="ON449_001084"
     CDS             complement(23193..24542)
                     /locus_tag="ON449_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3933685.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24612..25775)
                     /locus_tag="ON449_001085"
     CDS             complement(24612..25775)
                     /locus_tag="ON449_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3933686.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25904..26227
                     /locus_tag="ON449_001086"
     CDS             25904..26227
                     /locus_tag="ON449_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933687.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26410..27759)
                     /locus_tag="ON449_001087"
     CDS             complement(26410..27759)
                     /locus_tag="ON449_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933688.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27828..28781)
                     /locus_tag="ON449_001088"
     CDS             complement(27828..28781)
                     /locus_tag="ON449_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3933689.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28883..30073)
                     /locus_tag="ON449_001089"
     CDS             complement(28883..30073)
                     /locus_tag="ON449_001089"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3933690.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8517 a         6667 c         5334 g         9829 t
ORIGIN      
        1 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
       61 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
      121 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
      181 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
      241 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
      301 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
      361 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
      421 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
      481 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
      541 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
      601 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
      661 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
      721 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
      781 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
      841 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
      901 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
      961 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     1021 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     1081 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     1141 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     1201 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     1261 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     1321 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     1381 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     1441 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     1501 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     1561 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     1621 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     1681 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     1741 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     1801 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     1861 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     1921 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     1981 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     2041 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     2101 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     2161 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     2221 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     2281 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     2341 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     2401 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     2461 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     2521 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     2581 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     2641 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     2701 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     2761 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     2821 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     2881 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     2941 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     3001 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     3061 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     3121 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     3181 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     3241 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     3301 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     3361 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     3421 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     3481 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     3541 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     3601 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     3661 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     3721 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     3781 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     3841 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     3901 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     3961 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     4021 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     4081 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     4141 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     4201 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     4261 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     4321 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     4381 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     4441 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     4501 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     4561 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     4621 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     4681 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     4741 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     4801 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     4861 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     4921 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     4981 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     5041 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     5101 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     5161 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     5221 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     5281 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     5341 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     5401 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     5461 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     5521 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
     5581 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
     5641 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
     5701 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
     5761 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
     5821 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
     5881 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
     5941 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
     6001 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
     6061 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
     6121 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
     6181 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
     6241 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
     6301 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
     6361 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
     6421 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
     6481 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
     6541 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
     6601 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
     6661 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
     6721 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
     6781 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
     6841 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
     6901 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
     6961 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
     7021 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
     7081 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
     7141 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
     7201 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
     7261 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
     7321 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
     7381 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
     7441 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
     7501 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
     7561 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
     7621 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
     7681 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
     7741 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
     7801 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
     7861 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
     7921 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
     7981 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
     8041 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
     8101 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
     8161 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
     8221 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
     8281 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
     8341 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
     8401 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
     8461 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
     8521 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
     8581 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
     8641 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
     8701 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
     8761 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
     8821 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
     8881 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
     8941 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
     9001 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
     9061 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
     9121 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
     9181 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
     9241 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
     9301 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
     9361 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
     9421 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
     9481 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
     9541 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
     9601 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
     9661 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
     9721 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
     9781 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
     9841 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
     9901 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
     9961 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    10021 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    10081 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    10141 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    10201 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    10261 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    10321 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    10381 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    10441 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    10501 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    10561 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    10621 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    10681 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    10741 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    10801 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    10861 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    10921 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    10981 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    11041 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    11101 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    11161 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    11221 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    11281 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    11341 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    11401 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    11461 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    11521 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    11581 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    11641 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    11701 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    11761 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    11821 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    11881 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    11941 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    12001 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    12061 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    12121 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    12181 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    12241 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    12301 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    12361 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    12421 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    12481 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    12541 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    12601 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    12661 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    12721 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    12781 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    12841 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    12901 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    12961 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    13021 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    13081 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    13141 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    13201 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    13261 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    13321 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    13381 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    13441 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    13501 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    13561 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    13621 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    13681 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    13741 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    13801 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    13861 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    13921 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    13981 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    14041 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    14101 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    14161 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    14221 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    14281 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    14341 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    14401 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    14461 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    14521 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    14581 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    14641 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    14701 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    14761 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    14821 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    14881 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    14941 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    15001 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    15061 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    15121 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    15181 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    15241 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    15301 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    15361 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    15421 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    15481 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    15541 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    15601 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    15661 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    15721 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    15781 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    15841 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    15901 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    15961 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    16021 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    16081 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    16141 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    16201 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    16261 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    16321 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    16381 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    16441 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    16501 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    16561 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    16621 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    16681 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    16741 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    16801 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    16861 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    16921 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    16981 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    17041 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    17101 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    17161 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    17221 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    17281 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    17341 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    17401 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    17461 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    17521 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    17581 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    17641 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    17701 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    17761 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    17821 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    17881 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    17941 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    18001 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    18061 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    18121 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    18181 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    18241 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    18301 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    18361 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    18421 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    18481 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    18541 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    18601 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    18661 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    18721 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    18781 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    18841 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    18901 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    18961 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    19021 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    19081 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    19141 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    19201 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    19261 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    19321 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    19381 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    19441 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    19501 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    19561 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    19621 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    19681 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    19741 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    19801 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    19861 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    19921 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    19981 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    20041 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    20101 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    20161 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    20221 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    20281 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    20341 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    20401 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    20461 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    20521 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    20581 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    20641 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    20701 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    20761 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    20821 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    20881 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    20941 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    21001 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    21061 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    21121 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    21181 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    21241 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    21301 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    21361 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    21421 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    21481 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    21541 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    21601 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    21661 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    21721 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    21781 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    21841 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    21901 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    21961 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    22021 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    22081 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    22141 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    22201 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    22261 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    22321 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    22381 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    22441 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    22501 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    22561 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    22621 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    22681 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    22741 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    22801 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    22861 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    22921 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    22981 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    23041 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    23101 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    23161 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    23221 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    23281 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    23341 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    23401 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    23461 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    23521 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    23581 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    23641 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    23701 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    23761 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    23821 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    23881 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    23941 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    24001 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    24061 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    24121 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    24181 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    24241 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    24301 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    24361 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    24421 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    24481 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    24541 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    24601 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    24661 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    24721 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    24781 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    24841 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    24901 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    24961 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    25021 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    25081 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    25141 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    25201 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    25261 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    25321 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    25381 aaataatatc ttaaataaat gccaaatggt atgagtaata aaacattaaa agctggttcc
    25441 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    25501 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    25561 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    25621 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    25681 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    25741 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    25801 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    25861 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    25921 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    25981 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    26041 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    26101 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    26161 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    26221 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    26281 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    26341 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    26401 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    26461 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    26521 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtctaaacaa
    26581 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    26641 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    26701 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    26761 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    26821 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    26881 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    26941 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    27001 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    27061 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    27121 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    27181 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    27241 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    27301 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    27361 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    27421 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    27481 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    27541 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    27601 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    27661 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    27721 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    27781 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    27841 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    27901 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    27961 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    28021 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    28081 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    28141 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    28201 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    28261 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    28321 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    28381 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    28441 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    28501 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    28561 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    28621 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    28681 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    28741 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    28801 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    28861 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    28921 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    28981 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    29041 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    29101 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    29161 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    29221 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    29281 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    29341 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    29401 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    29461 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    29521 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    29581 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    29641 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    29701 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    29761 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    29821 aaccattgat cgttcggact gctcccgctc ctacaccata agtttcggtt gcttcaatac
    29881 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    29941 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    30001 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    30061 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    30121 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    30181 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    30241 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    30301 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtatt
//
LOCUS       DAJSVP010000022        29103 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSVP010000022 DAJSVP010000000
VERSION     DAJSVP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.022"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1030)
                     /locus_tag="ON449_001090"
     CDS             complement(<1..1030)
                     /locus_tag="ON449_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3933691.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ON449_001091"
     CDS             complement(1112..1801)
                     /locus_tag="ON449_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3933692.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ON449_001092"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ON449_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3933693.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="ON449_001093"
     CDS             complement(2328..2831)
                     /locus_tag="ON449_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3933694.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ON449_001094"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ON449_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3933695.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ON449_001095"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ON449_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3933696.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ON449_001096"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ON449_001096"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3933697.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ON449_001097"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ON449_001097"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3933698.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="ON449_001098"
     CDS             complement(6443..7462)
                     /locus_tag="ON449_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3933699.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="ON449_001099"
     CDS             complement(7635..8540)
                     /locus_tag="ON449_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3933700.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ON449_001100"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ON449_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3933701.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ON449_001101"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ON449_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3933702.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ON449_001102"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ON449_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3933703.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="ON449_001103"
     CDS             9753..10397
                     /locus_tag="ON449_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3933704.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ON449_001104"
     CDS             complement(10453..10956)
                     /locus_tag="ON449_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3933705.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ON449_001105"
     CDS             11553..12518
                     /locus_tag="ON449_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3933706.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ON449_001106"
     CDS             complement(12617..13789)
                     /locus_tag="ON449_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933707.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ON449_001107"
     CDS             complement(13776..14921)
                     /locus_tag="ON449_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933708.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ON449_001108"
     CDS             complement(14935..15867)
                     /locus_tag="ON449_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933709.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ON449_001109"
     CDS             15984..17063
                     /locus_tag="ON449_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3933710.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ON449_001110"
     CDS             17060..17689
                     /locus_tag="ON449_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3933711.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ON449_001111"
     CDS             complement(17799..18026)
                     /locus_tag="ON449_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3933712.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ON449_001112"
     CDS             complement(18129..20717)
                     /locus_tag="ON449_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3933713.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ON449_001113"
     CDS             complement(20737..21396)
                     /locus_tag="ON449_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3933714.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ON449_001114"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ON449_001114"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3933715.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ON449_001115"
     CDS             complement(22169..23035)
                     /locus_tag="ON449_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3933716.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ON449_001116"
     CDS             23144..23785
                     /locus_tag="ON449_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3933717.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ON449_001117"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ON449_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3933718.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ON449_001118"
     CDS             complement(25226..26125)
                     /locus_tag="ON449_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3933719.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ON449_001119"
     CDS             complement(26127..26924)
                     /locus_tag="ON449_001119"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3933720.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ON449_001120"
     CDS             complement(26902..27606)
                     /locus_tag="ON449_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3933721.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ON449_001121"
     CDS             27737..28312
                     /locus_tag="ON449_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3933722.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="ON449_001122"
     CDS             28430..>29103
                     /locus_tag="ON449_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3933723.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DAJSVP010000023        27817 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSVP010000023 DAJSVP010000000
VERSION     DAJSVP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.023"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(424..1368)
                     /locus_tag="ON449_001123"
     CDS             complement(424..1368)
                     /locus_tag="ON449_001123"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3933724.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2466)
                     /locus_tag="ON449_001124"
                     /pseudo
     CDS             complement(1664..2466)
                     /locus_tag="ON449_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2687..3901
                     /locus_tag="ON449_001125"
     CDS             2687..3901
                     /locus_tag="ON449_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3933725.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3923..5938
                     /locus_tag="ON449_001126"
     CDS             3923..5938
                     /locus_tag="ON449_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR3933726.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6021..7394)
                     /locus_tag="ON449_001127"
     CDS             complement(6021..7394)
                     /locus_tag="ON449_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3933727.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7420..7704)
                     /locus_tag="ON449_001128"
     CDS             complement(7420..7704)
                     /locus_tag="ON449_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3933728.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7706..9721)
                     /locus_tag="ON449_001129"
     CDS             complement(7706..9721)
                     /locus_tag="ON449_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3933729.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9900..10205)
                     /locus_tag="ON449_001130"
     CDS             complement(9900..10205)
                     /locus_tag="ON449_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3933730.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10294..11325)
                     /locus_tag="ON449_001131"
     CDS             complement(10294..11325)
                     /locus_tag="ON449_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3933731.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11532..12407
                     /locus_tag="ON449_001132"
     CDS             11532..12407
                     /locus_tag="ON449_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3933732.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12559..13401)
                     /locus_tag="ON449_001133"
     CDS             complement(12559..13401)
                     /locus_tag="ON449_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3933733.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13493..15370)
                     /locus_tag="ON449_001134"
     CDS             complement(13493..15370)
                     /locus_tag="ON449_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3933734.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15367..16788)
                     /locus_tag="ON449_001135"
     CDS             complement(15367..16788)
                     /locus_tag="ON449_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3933735.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16791..20180)
                     /locus_tag="ON449_001136"
     CDS             complement(16791..20180)
                     /locus_tag="ON449_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3933736.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20437..21828
                     /locus_tag="ON449_001137"
     CDS             20437..21828
                     /locus_tag="ON449_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933737.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21901..22548)
                     /locus_tag="ON449_001138"
     CDS             complement(21901..22548)
                     /locus_tag="ON449_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3933738.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22556..23242)
                     /locus_tag="ON449_001139"
     CDS             complement(22556..23242)
                     /locus_tag="ON449_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933739.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23248..24321)
                     /locus_tag="ON449_001140"
     CDS             complement(23248..24321)
                     /locus_tag="ON449_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933740.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24543..25217
                     /locus_tag="ON449_001141"
     CDS             24543..25217
                     /locus_tag="ON449_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3933741.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25222..26562
                     /locus_tag="ON449_001142"
     CDS             25222..26562
                     /locus_tag="ON449_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3933742.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26711..27697)
                     /locus_tag="ON449_001143"
     CDS             complement(26711..27697)
                     /locus_tag="ON449_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3933743.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8120 a         5238 c         5046 g         9413 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagg tgtttactga tatgaatatt
     2341 tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa
     2401 gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt
     2461 acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt
     2521 ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca
     2581 taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc
     2641 acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa
     2701 aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt
     2761 cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga
     2821 attattaacg actgctccct cttttatggt cactattttt gttttgatta gtagttttat
     2881 tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg
     2941 tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact
     3001 aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca
     3061 cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc
     3121 tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt
     3181 ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac
     3241 aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa
     3301 aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat
     3361 taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat
     3421 cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac
     3481 aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt
     3541 gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc
     3601 tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc
     3661 ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc
     3721 atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt
     3781 caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac
     3841 gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata
     3901 aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta
     3961 tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg
     4021 ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag
     4081 atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt
     4141 agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc
     4201 aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga
     4261 cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg
     4321 ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga
     4381 atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg
     4441 agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct
     4501 atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat
     4561 aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga
     4621 ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca
     4681 agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc
     4741 cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg
     4801 gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat
     4861 caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac
     4921 tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa
     4981 agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat
     5041 ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga tatttattga
     5101 ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc
     5161 ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa
     5221 ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta
     5281 cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt
     5341 gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca
     5401 atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt
     5461 caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac
     5521 gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata
     5581 cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag
     5641 cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt
     5701 gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga
     5761 aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga
     5821 taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta
     5881 tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa
     5941 caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca
     6001 atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg
     6061 gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag
     6121 gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta
     6181 tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc
     6241 agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca
     6301 gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga
     6361 atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag
     6421 ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct
     6481 aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt
     6541 tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca
     6601 gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat
     6661 ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat
     6721 acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg
     6781 tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta
     6841 tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca
     6901 ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt
     6961 gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct
     7021 tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc
     7081 atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact
     7141 actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca
     7201 aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca
     7261 aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca
     7321 agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc
     7381 aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt
     7441 cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc
     7501 atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat
     7561 atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc
     7621 aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact
     7681 ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt
     7741 cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg
     7801 ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca
     7861 aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag
     7921 acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc
     7981 caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt
     8041 cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt
     8101 tttttggttg atccgataat tggatcattt cttctgttaa taattcagat agcataggac
     8161 gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct
     8221 tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt
     8281 gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct
     8341 gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag
     8401 taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta
     8461 agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac
     8521 tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc
     8581 tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac
     8641 caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc
     8701 ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta
     8761 aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca
     8821 ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt
     8881 ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg
     8941 atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc
     9001 cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt
     9061 ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta
     9121 attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca
     9181 aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa
     9241 atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata
     9301 ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt
     9361 ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct
     9421 gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat
     9481 aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa
     9541 atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat
     9601 attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg
     9661 ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca
     9721 cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt
     9781 ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg
     9841 atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat
     9901 caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca
     9961 accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct
    10021 acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca
    10081 gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct
    10141 tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt
    10201 gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg
    10261 cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat
    10321 gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac
    10381 tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc
    10441 aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac
    10501 accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt
    10561 cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac
    10621 gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt
    10681 tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg
    10741 tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg
    10801 acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac
    10861 ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct
    10921 ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg
    10981 gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc
    11041 tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc
    11101 ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag
    11161 atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg
    11221 ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata
    11281 gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt
    11341 ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg
    11401 gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt
    11461 aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg
    11521 gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag
    11581 agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg
    11641 aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa
    11701 tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga
    11761 ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc
    11821 agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc
    11881 agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct
    11941 ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca
    12001 agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca
    12061 atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg
    12121 aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc
    12181 agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg
    12241 attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt
    12301 tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac
    12361 aagaattgat gcaaaagggc cttcttaaag aatatcggaa aacttaagaa aagatcctca
    12421 tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc
    12481 tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat
    12541 tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata
    12601 tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt
    12661 gttctcggat actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg
    12721 ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac
    12781 gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat
    12841 ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa
    12901 agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat
    12961 aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc
    13021 tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg
    13081 gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc
    13141 ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt
    13201 tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg
    13261 ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa
    13321 tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag
    13381 tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa
    13441 aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct
    13501 gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac
    13561 cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg
    13621 gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa
    13681 atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt
    13741 acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg
    13801 ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat
    13861 atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt
    13921 ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg
    13981 acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt
    14041 tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag
    14101 gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag
    14161 tgacagaata atttactggg tcgatttctt tatttccatc atataaggca taagcatacg
    14221 ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg
    14281 ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa
    14341 tctcatattg aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt
    14401 tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca
    14461 ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta
    14521 cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa
    14581 tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt
    14641 tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt
    14701 ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt
    14761 cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt
    14821 tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt
    14881 gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt
    14941 cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag
    15001 ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca
    15061 aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa
    15121 attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga
    15181 attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct
    15241 ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc
    15301 tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat
    15361 gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc
    15421 gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa
    15481 tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc
    15541 agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg
    15601 tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc
    15661 atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt
    15721 ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa
    15781 tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga
    15841 gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt
    15901 gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc
    15961 atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat
    16021 gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa
    16081 cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac
    16141 aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg
    16201 ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata
    16261 caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat
    16321 attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt
    16381 atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt
    16441 aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat
    16501 ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt
    16561 gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt
    16621 ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg
    16681 taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt
    16741 aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc
    16801 cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact
    16861 agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc
    16921 gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct
    16981 tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga
    17041 ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt
    17101 tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt
    17161 ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc
    17221 gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc
    17281 tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt
    17341 cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt
    17401 gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga
    17461 taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct
    17521 ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt
    17581 tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc
    17641 gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct
    17701 ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga
    17761 agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat
    17821 ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag
    17881 tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg
    17941 tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg
    18001 tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca
    18061 atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat
    18121 ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga
    18181 tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg
    18241 actccgggag ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt
    18301 ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt
    18361 tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc
    18421 tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt
    18481 gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga
    18541 tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat
    18601 tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat
    18661 gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg
    18721 ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct
    18781 tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt
    18841 gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac
    18901 tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat
    18961 gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc
    19021 atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt
    19081 gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta
    19141 ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata
    19201 ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga
    19261 ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata
    19321 tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt
    19381 tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata
    19441 tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt
    19501 gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg
    19561 gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat
    19621 ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg
    19681 ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct
    19741 ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt
    19801 gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg
    19861 tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct
    19921 tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt
    19981 tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc
    20041 gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt
    20101 gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt
    20161 ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat
    20221 agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt
    20281 attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat
    20341 gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa
    20401 catatgatca ggttgttgga atgaaactat agaaagatgg aaaaaatcat tcgaattctg
    20461 tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa
    20521 tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt
    20581 caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat
    20641 gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc
    20701 aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt
    20761 acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt
    20821 gaccaaggtt ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc
    20881 tttttctttt taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag
    20941 atcaaagaaa ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca
    21001 tatgagcaat ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga
    21061 actccatttg aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa
    21121 gacttcttaa tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta
    21181 gtgaactatt ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc
    21241 agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa
    21301 aaaaacgaag aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc
    21361 ccatacgata cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac
    21421 cgccagatac gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg
    21481 aatcagaatc tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct
    21541 gaaattcacc attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga
    21601 catagtcaat atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct
    21661 gagacaactg ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg
    21721 atcgttacga atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt
    21781 gtgtcattcg caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa
    21841 cagctatgta taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt
    21901 ttattgtatt ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc
    21961 ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa
    22021 gtataggaaa ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa
    22081 ttccagccat ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg
    22141 ctgatcattt cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa
    22201 gtcgcagcac cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct
    22261 aatgaatttt aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata
    22321 agcatcttcc aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc
    22381 aacgatttct gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag
    22441 gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca
    22501 catctctcct ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt
    22561 ccttcttttt ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc
    22621 attgaatcat acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt
    22681 ctttaactag taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt
    22741 cgtctgctaa gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc
    22801 tttctccacc agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt
    22861 gcagcaagga atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt
    22921 caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta
    22981 tttctttaaa gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt
    23041 tgatcactat ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac
    23101 ttttacctga accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat
    23161 caattgcttt caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt
    23221 tcatcgttaa tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt
    23281 ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact
    23341 atatagcaac cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact
    23401 agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg
    23461 tgctactaaa gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa
    23521 aatagcagtc ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac
    23581 taagaacaaa aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat
    23641 actttcaatg aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga
    23701 aattttagct gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa
    23761 cggctgttgt ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga
    23821 aactgtgtaa taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc
    23881 aattttgatc gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat
    23941 ttcattcttt gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt
    24001 cgttgtgccg aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa
    24061 tccgattggt gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga
    24121 agctgcaaat acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc
    24181 gattgctttt ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac
    24241 atacgcaatc aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat
    24301 ttctttgata gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat
    24361 gtcacttgtt gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct
    24421 tatcaataaa ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt
    24481 atttaagcct tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga
    24541 aaatgattaa actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa
    24601 tactcgctgc aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt
    24661 tggaaagtgg agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat
    24721 atgaagtgtt agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg
    24781 caaaagatag tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa
    24841 caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc
    24901 taggtctctt tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca
    24961 atgaagtctt gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg
    25021 tttatttttt acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct
    25081 ggggatttga ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa
    25141 aacatctgcg aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata
    25201 tactgcaaag ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt
    25261 tttgcgacaa atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta
    25321 cagatattaa accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt
    25381 gattctcgaa cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac
    25441 ccgactcaaa cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat
    25501 gaccgtttta acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca
    25561 gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat
    25621 accatccaaa atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg
    25681 attacgaaaa aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat
    25741 acaaatcaaa tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt
    25801 gttttttgga ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct
    25861 attattcttt cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc
    25921 acaccgttaa cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact
    25981 attattgaag aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga
    26041 ttgacttcgg atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag
    26101 caaatgatca atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc
    26161 atcatagatg gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat
    26221 gaaaaaaaaa tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat
    26281 ccagaagata agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta
    26341 aagaatactg gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat
    26401 cgggaagatc aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca
    26461 aaacaggtaa tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga
    26521 gtcgatttta aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag
    26581 ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag
    26641 tagcttcttc ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt
    26701 tagcttacgc ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa
    26761 tatccaattc ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt
    26821 tatgaatccc aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt
    26881 tgattgggat agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat
    26941 ctgttactgg tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt
    27001 cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat
    27061 ttacatctaa tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat
    27121 cgatgtcttc agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat
    27181 cagttcctgg tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta
    27241 cgtccattgg atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc
    27301 taacagtatc ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta
    27361 ctgtgatgac aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt
    27421 ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc
    27481 ataaagaacg gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag
    27541 acaatccgtc aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat
    27601 ctcgttcata cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac
    27661 tatagcttcc gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga
    27721 tttatgattg gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca
    27781 aatatttaga ttggaatttg attaaaacaa tttaatt
//
LOCUS       DAJSVP010000024        27694 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSVP010000024 DAJSVP010000000
VERSION     DAJSVP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.024"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            130..1389
                     /gene="lysA"
                     /locus_tag="ON449_001144"
     CDS             130..1389
                     /gene="lysA"
                     /locus_tag="ON449_001144"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3933744.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1376..2176
                     /gene="dapF"
                     /locus_tag="ON449_001145"
     CDS             1376..2176
                     /gene="dapF"
                     /locus_tag="ON449_001145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3933745.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2179..3060
                     /locus_tag="ON449_001146"
     CDS             2179..3060
                     /locus_tag="ON449_001146"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3933746.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3057..3773
                     /locus_tag="ON449_001147"
     CDS             3057..3773
                     /locus_tag="ON449_001147"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3933747.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3779..4474
                     /gene="dapD"
                     /locus_tag="ON449_001148"
     CDS             3779..4474
                     /gene="dapD"
                     /locus_tag="ON449_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3933748.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4490..5491
                     /locus_tag="ON449_001149"
     CDS             4490..5491
                     /locus_tag="ON449_001149"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3933749.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5488..6849
                     /locus_tag="ON449_001150"
     CDS             5488..6849
                     /locus_tag="ON449_001150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3933750.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6809..7960
                     /locus_tag="ON449_001151"
     CDS             6809..7960
                     /locus_tag="ON449_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3933751.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7935..9104
                     /locus_tag="ON449_001152"
     CDS             7935..9104
                     /locus_tag="ON449_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3933752.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9279..10658
                     /locus_tag="ON449_001153"
     CDS             9279..10658
                     /locus_tag="ON449_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3933753.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10939..11736
                     /locus_tag="ON449_001154"
                     /pseudo
     CDS             10939..11736
                     /locus_tag="ON449_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11925..12593
                     /locus_tag="ON449_001155"
     CDS             11925..12593
                     /locus_tag="ON449_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3933754.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12793..13179)
                     /locus_tag="ON449_001156"
     CDS             complement(12793..13179)
                     /locus_tag="ON449_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933755.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13151..14533)
                     /locus_tag="ON449_001157"
     CDS             complement(13151..14533)
                     /locus_tag="ON449_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3933756.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14689..15876)
                     /locus_tag="ON449_001158"
     CDS             complement(14689..15876)
                     /locus_tag="ON449_001158"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3933757.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15883..16914)
                     /locus_tag="ON449_001159"
     CDS             complement(15883..16914)
                     /locus_tag="ON449_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3933758.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16930..18384)
                     /locus_tag="ON449_001160"
     CDS             complement(16930..18384)
                     /locus_tag="ON449_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3933759.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            18559..19464
                     /locus_tag="ON449_001161"
     CDS             18559..19464
                     /locus_tag="ON449_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3933760.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19555..20232
                     /locus_tag="ON449_001162"
     CDS             19555..20232
                     /locus_tag="ON449_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3933761.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20229..20399
                     /locus_tag="ON449_001163"
     CDS             20229..20399
                     /locus_tag="ON449_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933762.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20513..21409
                     /locus_tag="ON449_001164"
     CDS             20513..21409
                     /locus_tag="ON449_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3933763.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21464..22834)
                     /locus_tag="ON449_001165"
     CDS             complement(21464..22834)
                     /locus_tag="ON449_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933764.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23072..23818
                     /locus_tag="ON449_001166"
     CDS             23072..23818
                     /locus_tag="ON449_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3933765.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23815..24654
                     /locus_tag="ON449_001167"
     CDS             23815..24654
                     /locus_tag="ON449_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3933766.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24655..25605
                     /locus_tag="ON449_001168"
     CDS             24655..25605
                     /locus_tag="ON449_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3933767.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25746..26498
                     /locus_tag="ON449_001169"
     CDS             25746..26498
                     /locus_tag="ON449_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933768.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26517..27668
                     /gene="queG"
                     /locus_tag="ON449_001170"
     CDS             26517..27668
                     /gene="queG"
                     /locus_tag="ON449_001170"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3933769.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9099 a         5044 c         5823 g         7728 t
ORIGIN      
        1 taaaaaaata tttcgaaata tactagacaa ttcagaaaat tttgtttaat attattccaa
       61 taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg
      121 aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag
      181 tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc
      241 aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc taaagcattc
      301 caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt
      361 ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat ctacttccat
      421 ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt
      481 gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa
      541 aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc
      601 gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa
      661 gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca tgctcatatc
      721 gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac
      781 ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat
      841 acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg
      901 gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga accgggtaga
      961 agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca
     1021 cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc agctctgtat
     1081 caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg
     1141 acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg
     1201 aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg
     1261 agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca
     1321 agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat
     1381 ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac
     1441 agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata cagacggatt
     1501 gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc
     1561 gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat
     1621 gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga
     1681 tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat
     1741 caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt
     1801 aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg aaaatgcgcg
     1861 tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa
     1921 aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga
     1981 aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc
     2041 taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt
     2101 gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag
     2161 aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat
     2221 gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa
     2281 caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct
     2341 gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt
     2401 gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct
     2461 ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga
     2521 ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat
     2581 gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat
     2641 cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct
     2701 cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta
     2761 atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc
     2821 catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta
     2881 aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca
     2941 gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca
     3001 aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga
     3061 aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa
     3121 gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta
     3181 cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt
     3241 cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat acacaatcac
     3301 agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt
     3361 cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct
     3421 ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg
     3481 caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc
     3541 aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac
     3601 ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat
     3661 ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc
     3721 agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt
     3781 ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt
     3841 ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag
     3901 cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga
     3961 aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga
     4021 tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat
     4081 cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga
     4141 aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg
     4201 ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt
     4261 tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat
     4321 cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcagaaac
     4381 tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa
     4441 tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat
     4501 agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga
     4561 agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa
     4621 agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga
     4681 aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat
     4741 cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga
     4801 tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc
     4861 aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt
     4921 tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg
     4981 cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc cttataatat
     5041 tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga
     5101 acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc
     5161 aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca
     5221 aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt
     5281 agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat
     5341 ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac
     5401 gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt
     5461 aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc
     5521 ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat
     5581 cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa
     5641 gtaacagact tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc
     5701 ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta
     5761 cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa
     5821 gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt
     5881 taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag
     5941 gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat ggttgttcca
     6001 gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac
     6061 attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat
     6121 gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa
     6181 atctctttta gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca
     6241 gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct
     6301 ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga
     6361 atagctggac gcaaggattt tctttccatc actttattca aacgtcatat gtccgatgaa
     6421 atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat
     6481 attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataag
     6541 ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatc
     6601 gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat tggattatct
     6661 gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga
     6721 gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac agtagtgaag
     6781 ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca
     6841 aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa
     6901 aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa
     6961 tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga
     7021 aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg gtcatgacgg
     7081 acatatggcg atgcttttag ggttgactga ctttataact aaacacattg aggatcttat
     7141 ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt
     7201 tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg
     7261 gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag
     7321 tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat
     7381 cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt
     7441 accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgaaa
     7501 tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt cagatatgca
     7561 aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta cagctactat
     7621 ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc
     7681 actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt
     7741 tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc
     7801 tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg
     7861 gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga
     7921 ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt tctagaatag
     7981 aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac
     8041 tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca ggaatagcag
     8101 cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag
     8161 cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt
     8221 tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag
     8281 gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag
     8341 ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt cagccggaac
     8401 aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc
     8461 ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt
     8521 taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg
     8581 gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat
     8641 ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta
     8701 ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa
     8761 aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat
     8821 atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc
     8881 agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt
     8941 ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat
     9001 ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac
     9061 atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata gattttatca
     9121 aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt tttaaattta
     9181 gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt taaattgttt
     9241 agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca
     9301 ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca cgatacggtg
     9361 aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg tcatgtggta
     9421 tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat ttcagaccag
     9481 aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa acgatcgatg
     9541 ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa gaatatcctt
     9601 atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta ccaggagcag
     9661 atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc aaagaaaaat
     9721 taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag
     9781 cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg atcgatagac
     9841 ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac ctaaatgaaa
     9901 acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg
     9961 aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa gctgccggtg
    10021 tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc
    10081 aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga gatgctacac
    10141 ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta
    10201 gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg
    10261 gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca agcggattga
    10321 atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat gaagatacgt
    10381 tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc caattattct
    10441 cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat gatgttacag
    10501 gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa gaactagcag
    10561 aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg aatctagccg
    10621 ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata tttgaaaaga
    10681 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa acagcttctt
    10741 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattt
    10801 tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc
    10861 taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta aattaaagat
    10921 cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga tcaagagatt
    10981 gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt acggatgacc
    11041 aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg cttgatcaag
    11101 aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga
    11161 ggaaaagttg gagcagtagc caaaaatcgg atccatcgtc gtttctacac cagtatctgc
    11221 catcagaaaa tcacgacgga tacaactgaa tttaaatacc atgaagtgga tgccaaaggc
    11281 attatccgtc aaaagaaatt gtatctagat ccttttatgg atatgttcaa tagtgagatt
    11341 ttatcctatc gtatctctga aaagcccaat gcattagcgg taatggaagg tttggaggaa
    11401 gctatccaga ccactaacga ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg
    11461 gcgtatcaaa tgaaagctta tagaaacaaa ttgaaggagt acaaaatctt tcaaagcatg
    11521 tctcggaaag ggaactgctt agataattca cttatggaga atttttttgg cttattaaaa
    11581 caagagattt ttcacgggaa agtttataac tgtttcgagg aattaaaatc agccatcgat
    11641 agctatatct attactacaa taatgaacgg ataaaacaga aattaaactg gcaaagtctc
    11701 gtccaatttc gtaaaacaac cgtaacggtt gtgtaaaaat atcattttag agcataaaaa
    11761 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acagtgtcac
    11821 agtccttttt ttagtaaaag gatacatttc ttccttgtat attccatgcg tgttcgtgag
    11881 aaagcatata taatttaatt aacagatagt tagggggaga aaaaatgatt ttttcaatta
    11941 caatgaaact ggtgttcggg ttgatcggtt tattattagt ggtacgtttg cttgggaaga
    12001 aatccatgtc agaaatcaca ccgtttgatt tagtctatac gcttgtttta ggtgggatct
    12061 tagaagaatc aacctatgat gacaatgtcc atgtagggca tgtcctattt gccattgcgc
    12121 tttgggcagt aatgatttat ggtatcgaac gtatcgtcca aaaaaatgaa aaggtcaatc
    12181 gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg cgtcatcaac atgacagagt
    12241 tgacaaataa ccatatcgag atggagcagt tgcgagcaat cttaagacag caagaatgct
    12301 tttctcttga aaatgccaaa catgttatcc tagagaatgc cggtcaaatg agtgtattga
    12361 aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt agatgaaggg caaattcagc
    12421 ataaagtgct tcagtccaat caattgactg aagcatggct tatggagaac ttgaagaaag
    12481 aaggctatgc agatgtaaaa cagctgattt atgttgaatg gtcagaagaa aaagggctct
    12541 acatactaaa taaagaagac gtcatgcatg aatcttaccg aatagatgga tagatagaca
    12601 gaggttgtga aatcagcgcc ttggcttgtt actcaaaact aagcgacttt ttcacaacct
    12661 ctgatatcta atatacgttg ttccagaagt tgcttcttcg aaaaataagc caaaattcca
    12721 caaaaattta agaagcaatt ttcgaaaaat cctttcttat tttttgaagt tgatcacttc
    12781 tgtcacaacc tctcatgtca caaaccattt ttttgtttcc tttaactcaa tataccgtac
    12841 gtcttcccac aatatcggct gatggtcaat cataaagtaa tcttcatagg cttcaccaat
    12901 gaagtgccct tcgacctcat ccaagagtct tccaaattca tcttttaaat tgagctgaat
    12961 gattactgct ttacgagata aaaaggcttc tgtcagtata ctatcgattt cttgcaatgt
    13021 catttgttgt ttaggaagag gctgtttgcg tgcttcttct tcattttttt tgataccgtt
    13081 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
    13141 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
    13201 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
    13261 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
    13321 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
    13381 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
    13441 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
    13501 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
    13561 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
    13621 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
    13681 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
    13741 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
    13801 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
    13861 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
    13921 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
    13981 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
    14041 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
    14101 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
    14161 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
    14221 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
    14281 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
    14341 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
    14401 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
    14461 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
    14521 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
    14581 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
    14641 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
    14701 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
    14761 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
    14821 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
    14881 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
    14941 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
    15001 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
    15061 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
    15121 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
    15181 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
    15241 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
    15301 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
    15361 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
    15421 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
    15481 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaaca actcaaaccc
    15541 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
    15601 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
    15661 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
    15721 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
    15781 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
    15841 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
    15901 gattttgaat tctgtcactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
    15961 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
    16021 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
    16081 cacactagaa gcatctgtag caatacctac ttggattctt ttatcggggc tagtcaagcg
    16141 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
    16201 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
    16261 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
    16321 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
    16381 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
    16441 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
    16501 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
    16561 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
    16621 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
    16681 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
    16741 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
    16801 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
    16861 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
    16921 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
    16981 tggagccaat ttgttttcca attcctcaac gatttgtgca tgttttttct ctgtcaatgt
    17041 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
    17101 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
    17161 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
    17221 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
    17281 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
    17341 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
    17401 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
    17461 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
    17521 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
    17581 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
    17641 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
    17701 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
    17761 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
    17821 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
    17881 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
    17941 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
    18001 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
    18061 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
    18121 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
    18181 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
    18241 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
    18301 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
    18361 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
    18421 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
    18481 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
    18541 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
    18601 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
    18661 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
    18721 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
    18781 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
    18841 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
    18901 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
    18961 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
    19021 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
    19081 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
    19141 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
    19201 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
    19261 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
    19321 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
    19381 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
    19441 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
    19501 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
    19561 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
    19621 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
    19681 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
    19741 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
    19801 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
    19861 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
    19921 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
    19981 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
    20041 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
    20101 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
    20161 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
    20221 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
    20281 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
    20341 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
    20401 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
    20461 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
    20521 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
    20581 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
    20641 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
    20701 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
    20761 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
    20821 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
    20881 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
    20941 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
    21001 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
    21061 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
    21121 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
    21181 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
    21241 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
    21301 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
    21361 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
    21421 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
    21481 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
    21541 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
    21601 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
    21661 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    21721 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    21781 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    21841 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    21901 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    21961 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    22021 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    22081 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    22141 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    22201 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    22261 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    22321 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    22381 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    22441 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    22501 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    22561 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    22621 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    22681 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    22741 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    22801 cactaagact tcgagttaac tttttttctt ccatacaaaa actcctttcg ttgttgagtc
    22861 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    22921 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    22981 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    23041 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    23101 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    23161 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    23221 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agccggtaaa
    23281 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    23341 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    23401 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    23461 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    23521 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    23581 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    23641 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    23701 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    23761 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    23821 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    23881 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    23941 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    24001 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    24061 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    24121 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    24181 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    24241 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    24301 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    24361 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    24421 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    24481 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    24541 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    24601 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    24661 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    24721 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    24781 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    24841 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    24901 gcaagtgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    24961 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    25021 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    25081 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    25141 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    25201 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    25261 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    25321 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    25381 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    25441 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    25501 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    25561 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    25621 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    25681 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    25741 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    25801 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    25861 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    25921 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    25981 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    26041 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    26101 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    26161 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    26221 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    26281 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    26341 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    26401 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    26461 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    26521 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    26581 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    26641 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    26701 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    26761 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    26821 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    26881 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    26941 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    27001 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    27061 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    27121 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    27181 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    27241 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    27301 tggaaccaaa agtagacgaa gtatatccaa aattaaagcc aatgctttcg atgtctaata
    27361 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    27421 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    27481 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    27541 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    27601 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    27661 taaaataaaa aaatagttaa cattattcaa agtt
//
LOCUS       DAJSVP010000025        27085 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSVP010000025 DAJSVP010000000
VERSION     DAJSVP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.025"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            4..861
                     /locus_tag="ON449_001171"
     CDS             4..861
                     /locus_tag="ON449_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3933770.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="ON449_001172"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="ON449_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3933771.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="ON449_001173"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="ON449_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3933772.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="ON449_001174"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="ON449_001174"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3933773.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /locus_tag="ON449_001175"
     CDS             3617..4375
                     /locus_tag="ON449_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3933774.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="ON449_001176"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="ON449_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3933775.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5316..6334
                     /locus_tag="ON449_001177"
                     /pseudo
     CDS             5316..6334
                     /locus_tag="ON449_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            6331..7098
                     /locus_tag="ON449_001178"
     CDS             6331..7098
                     /locus_tag="ON449_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3933776.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7198..8766
                     /gene="liaX"
                     /locus_tag="ON449_001179"
     CDS             7198..8766
                     /gene="liaX"
                     /locus_tag="ON449_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3933777.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9063..9383
                     /locus_tag="ON449_001180"
     CDS             9063..9383
                     /locus_tag="ON449_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3933778.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9383..9733
                     /locus_tag="ON449_001181"
     CDS             9383..9733
                     /locus_tag="ON449_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3933779.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9900..10838
                     /locus_tag="ON449_001182"
     CDS             9900..10838
                     /locus_tag="ON449_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3933780.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10853..11683
                     /locus_tag="ON449_001183"
     CDS             10853..11683
                     /locus_tag="ON449_001183"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3933781.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11727..12752
                     /locus_tag="ON449_001184"
     CDS             11727..12752
                     /locus_tag="ON449_001184"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3933782.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12767..13705
                     /gene="galU"
                     /locus_tag="ON449_001185"
     CDS             12767..13705
                     /gene="galU"
                     /locus_tag="ON449_001185"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3933783.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13830..14270
                     /locus_tag="ON449_001186"
     CDS             13830..14270
                     /locus_tag="ON449_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3933784.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14273..14875
                     /locus_tag="ON449_001187"
     CDS             14273..14875
                     /locus_tag="ON449_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3933785.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14934..16040)
                     /locus_tag="ON449_001188"
     CDS             complement(14934..16040)
                     /locus_tag="ON449_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3933786.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16250..17251
                     /gene="ccpA"
                     /locus_tag="ON449_001189"
     CDS             16250..17251
                     /gene="ccpA"
                     /locus_tag="ON449_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3933787.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17588..19933)
                     /locus_tag="ON449_001190"
     CDS             complement(17588..19933)
                     /locus_tag="ON449_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3933788.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20212..21114
                     /locus_tag="ON449_001191"
     CDS             20212..21114
                     /locus_tag="ON449_001191"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3933789.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21327..23102
                     /locus_tag="ON449_001192"
     CDS             21327..23102
                     /locus_tag="ON449_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3933790.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23168..24754)
                     /locus_tag="ON449_001193"
     CDS             complement(23168..24754)
                     /locus_tag="ON449_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3933791.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25005..25229
                     /locus_tag="ON449_001194"
     CDS             25005..25229
                     /locus_tag="ON449_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3933792.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25371..26969
                     /locus_tag="ON449_001195"
                     /pseudo
     CDS             25371..26969
                     /locus_tag="ON449_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         8647 a         4833 c         5684 g         7921 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagccattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggacagggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 gtcacaacga tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc atatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcaa tctaagtttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcgggaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagtagc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa
     5101 gaggttgtga aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca
     5161 aacaaaaaat agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct
     5221 tttgaacacc gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat
     5281 catttcatag aaaggataaa gaatgcaaaa aaaaaattag catcatcact atatttttta
     5341 tttgtgtgct tacttcatgg gttattcagc atcaagctgc tttatatgaa gtacctgtca
     5401 tcgagatcga aaaagtacga ataaaagagg atcatcaatt gattacagga agactgatga
     5461 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
     5521 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
     5581 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
     5641 ccatgttgct tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
     5701 tcgttttact ttacttttgt tgctcatctt tactaaaaca gaagggattt ctctattaaa
     5761 tatatctatc ctgttcgtcg ttatagggat gattattagc atattggctt taaatggatg
     5821 gaatagaagc agtataatca aaatttcagc tgcagttacg agtgtttttt tagcattttt
     5881 tatttgtctt ttgacaatga atcattggaa agaccaagga ctgcgctatg aggaactgaa
     5941 ttatctgaca cgaccgtatc gttctgtttt tctttctagc gtactgattg gaacagctgg
     6001 aggaacactc gataccgtta tcacagtcat tgcgacatta gaagaattga ctaagcgaca
     6061 accagaaata acagcgaaac agttatggct ttcaggaagg caagtgggtc gagatgtgat
     6121 tggctcgatg accaatatcc ttttgttcgt ctatatcagt gggtcagttc catcgcttct
     6181 tttatattta ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt tatcattgga
     6241 atttttacgc gtcattgctg gcagcttagg aatagtatta tcgatcccta tctcggtcct
     6301 cttttttctg actgtaagga ggctgaaacg atgaatgtac ttattatact tgttgggatt
     6361 ttagcaatca gcgtgctact tattggcggt acgcaaggta tgtatattct tttaggctta
     6421 ttcatcaatt tagggatctt tttcctgttg ttattcggtt atcatcagaa gtggccgata
     6481 ctggcattgt cggtcatagg atttttattg attgctgtgg ttatcttatt ttttatcaac
     6541 ggttataatc taaaaatgcg tgcagctttt gtgagtatac tgatcttttt attttgtttc
     6601 ctactattgc ttcctatcac tgatttttta gcgatccaag gttttacatc ggtagaacta
     6661 gaagaattat ctggcttaga taagacttta gcgattgatt ttagattact tacgagatcg
     6721 ttgcttctga ttagtcttag tggtgctgtt ttagatgcta gtgtagcaat ttctagtggg
     6781 acattcgaag tatatcaggc aaatccgcaa ttatcttttt accaattgcg ccattctagt
     6841 ttcgctattg ctaaaaaaat attggcgtca acagtaatga cacttctttt tgcattcatg
     6901 ggaagttctt tagcgttgat cctctggttt attgatttag acattccttt tcagcaagtg
     6961 atcaatgaaa aatcatttgt tctagaatat acgatggcta ttcttaccac attgtctgca
     7021 ctacttgttc ttcctttaac ctgcgtgact acagcatatc tctttacaaa aaagaaagaa
     7081 caactcaaga tggagtaaaa gttacaactt aagtcctatg acatttttac tgaatcagtg
     7141 cataatatgg atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc aatttccatg
     7201 aaagaaagag aacgtatttt agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc
     7261 ttagatttat tagaaagcat ggcaactgaa aaagacgaaa agcaaataaa aaaagaagca
     7321 gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat cgataaactt
     7381 gaaaacggcg aagaagaaat ttctgagcca gttgatccaa aggaaagaga aagacaagat
     7441 caagaaaact tagaaaagat cttagatgaa ctagcaactg aagcgaataa aacttcggcg
     7501 cgtctagatg aagtaaatgc tcaattcgct gacaataagg cagcacgcaa tgagaaacag
     7561 gaagcattga tgcagctgaa tacaaaagaa gaattaggcg agttgacaga agaagaatta
     7621 gcacagcgtc agacgttaga agcagaaatc aaagaattag aagctagtgg cgatacactg
     7681 ttagaagaac aattgaaatt agaagcagaa ttaaaagata tccgtaagaa tcaatggtct
     7741 gagaaaaaag aaacgtttac tgataagttt gaattgccag atgattggaa agatcaagca
     7801 acagatacac tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa
     7861 ttcttgaaga aaacattcca aactgtttca gaaacagtga atgacaacat ggaatggaaa
     7921 gatgtcagct tacgtgtccc tgggattgcg acaacgaaat ttgagcatga attttactat
     7981 gaagctcctg cagcaagtat ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa
     8041 acgtgggaca gcaacgatgt gaaagttgaa gctaagatca aactttacgg aaaaatgggt
     8101 gctgaaccat ttgaagcatt ctctgaacgg agccagattg aggttaatga agatcatatc
     8161 tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg ttttctactt acctaaacga
     8221 gtatacgacc acgcagctat taaacttcta aatggcaaca tcatgattga aacattggaa
     8281 gctaaagata tttatacaaa atctacgaat ggtaacatca tcgtggacca attaactgca
     8341 acgatgctgg aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg gaacatctta
     8401 gattctatta tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc agaaaacctt
     8461 tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta aagaagataa tttgaaaaaa
     8521 gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg catcggtttg
     8581 gaaggacatg ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa ttacgaagta
     8641 gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt
     8701 gaaatcacgc aagtgcaact ttcaacgaca acaggaagta tctatttaaa agatacagat
     8761 aaataaagag gttgtgaaag agatgttcag ccttgagcaa taagaagcga ttttgtaaaa
     8821 tagcttcccc tattttacaa aattgtgcct tattgccgat aggctatctc ttgaacaccg
     8881 tttaccaaga gattatgaac ctgccaatta tcgccgagtc acaaggaata atttcagaaa
     8941 aagctcgtca cgttttatga atatggtaat ttgtgaccga gaattttgca gaaagacacc
     9001 gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag
     9061 acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca gggactttag
     9121 caggaatagg agaatacatc gggattgatc cgactgttat ccgtgtgctt tatgtattcc
     9181 tgagcttagt tgcttttgga agtccaatca ttttatacat tttattagct ttgattatcc
     9241 catctgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca
     9301 attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg
     9361 atgacgaatg gagtgacttt taatgtcgta ctttcaacgt attttagtta atacgttaac
     9421 attcatatca ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc
     9481 cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat
     9541 actgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc
     9601 gattcttaaa atgacctcct tttttgtggg agcagaacat tttagttttt cttcattcgg
     9661 tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa
     9721 cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc agttttttta tttagaatca
     9781 ggaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
     9841 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
     9901 tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt atctttggaa atcgtgacag
     9961 gcgacgagaa aagcttagac cgtgtcatcg taactgggga catctcacgc ccaggactag
    10021 agttgactgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    10081 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcatcatg agaagattat
    10141 gtagtgaaga tatgcccgca tttattattt caagaggatt accggctcca gaagaaatga
    10201 tccaggcagc aaatgaaaat gggatagctg tactgcgttc tcccatctca acttctcgtt
    10261 tattaggaga attatccagt tatttagatg gtcgccttgc ccctcgaaca agtgtccacg
    10321 gtgttttagt ggatgtttac ggattaggtg tactgattca aggtgacagt gggataggaa
    10381 aaagtgaaac agcgttggag ttgatcaaac gcggtcatcg attgatcgca gacgatcgag
    10441 tagatgttta tcagcaagat gaattaacgg tgatcggaga accgcctaaa atcctggaac
    10501 atctaattga aatccgagga atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg
    10561 cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg gcaaaagata
    10621 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    10681 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    10741 cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    10801 taagtcgctt gattgaagaa aactcaggca acgactagca aggagtgaca atatgttagc
    10861 acaaatcaat cgagttgctt ttgagcttgt agggattcct atctactggt atgcattgat
    10921 catcgtatca ggtatcatta tcgccatgtg gcttagcagc agagaggctg ttcgggtagg
    10981 attaaaagca gatgatgtga cagattttat gttgtgggga ttgcctatct ccattattgg
    11041 cgctcgctta tattatgttt tatttgatct tccccagtat atagctgatc caatccaaat
    11101 attcaatatc cgttcaggag gcttagctat ttatggcgga ctgattgcag gcgcgatcac
    11161 gctgtatttt tttactaaat atcattttat ttctttgtgg acttttttag atatcgctgc
    11221 accaagtgta ttattagcac aggcaatcgg tcgttgggga aatttcatga accacgaagc
    11281 atatggacca gacacgacgc gaagcttctt agaaagtctg catttaccgc aattcatcat
    11341 tgataatatg tatatcgaag ggatctatcg tcaaccaaca tttctttatg aatcactttg
    11401 gagtttgatt ggatttattt tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa
    11461 aggcgaagtc cttttatgtt atctaatgtg gtatagtttc gggcgtttct ttattgaagg
    11521 attacgtaca gacagtcttt actttttagg tgtgatccgt atttctcaat tattatctgc
    11581 tgtgttattt gtcggtacat tgattctatt tatttggcgc agaaaaaaag gaaatttacc
    11641 ggattatgac cgctcattag gaaaaaatca acaaattatc tgatttttag agcgttaaaa
    11701 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    11761 cttggggtac tgctttggca caagtcctcg ccgaaaacgg acatgacgtt cggatttggg
    11821 gacatcgtgc ttcacaagta gaagaaatca acacccaaca cacgaatcaa cgttatttac
    11881 cagatcatta tctacccgat tccatccaag cattcaccaa tatggaagat gcagtaaaag
    11941 acgcagatgc ggtgctattc gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac
    12001 tagtgcctaa attgaataca aagccagtga tcatccatgc aagtaaagga ttagaacagg
    12061 gaacacataa aaggatctct gacatcttgt tagaggaaat cccagcagaa aaaagaaaag
    12121 ctgttgttgt tctttctgga ccaagccatg cagaagaagt agcgaaacat gacatcacta
    12181 cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag ctgttcatga
    12241 ataattattt tcggatctat accaatcctg atgtgatcgg tgtagagatg ggggcagctt
    12301 tgaaaaatat tattgctatt ggtgctggcg ctattcacgg cttagggttt ggcgataatg
    12361 caaaggcagc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    12421 gtgccaaccc gctgaccttt atcggattaa gcggagtagg cgatctagtc gttacttgca
    12481 caagtgttca ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg cagcccctag
    12541 aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt gaatactacc caagcagcga
    12601 tggaactagc ccaaataatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    12661 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    12721 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtcaaaaa
    12781 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aagctatggc
    12841 aaaggaaatg ttgccaattg ttgataaacc aacaatccag ttcatcgttg aagaagcgtt
    12901 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    12961 tcactttgat tcaaatattg aattagaaac aaacctaagt gaaaaaggaa agaccgaatt
    13021 attgaaatta gtagaagaaa cgacagatgt caacttgcat ttcatccgtc aatctcatcc
    13081 aaagggttta ggacatgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    13141 cgttatgcta ggcgatgata tcatggaaga tgaagtacca ttgacacagc aattaatcga
    13201 tgattacgaa cgtactcatg cttctacgat cgccgttatg caagttcctc atgatgaaac
    13261 ttcaaaatac ggaattatca atccaggaga agtattggaa gatgggttat acaatgtcaa
    13321 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattggacg
    13381 ttacttattg actccagaaa tttttgatgt attgtccgag caacaaccag gagccggaaa
    13441 tgaaatccaa ctaactgacg caatcgatac attgaataaa acacaacgcg tatttgcccg
    13501 taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga aaacaagtat
    13561 tgaatatggt ctaacacatc cagaagtagg tgcaccttta cgtgagtata tcctgtcttt
    13621 aggtaaaaca ttagaagcag aaggaagcaa gatcgatcat aatccaaaag gaataacaga
    13681 aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg agcttcagct gctaattgaa
    13741 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    13801 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgattg
    13861 cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta
    13921 aaactttaga aaaagtagat cagactgtag cagaagcaaa tacgacgatc gaaatcgtca
    13981 caaaagacgt agatcttttg tctagacaag tagaaggctt gctggtcaaa agtaatgaac
    14041 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    14101 atttaagtga aagtgtatca gaacttaatc attctagtaa aaacatcgcg acgaaagtcg
    14161 gcggaatcgg taaagcgaca gctaaagcga cggttgccgg aaaagtcggt gggacagcga
    14221 tgaaattttt taaaaacaaa aaacatactg aaacgacagg aggaaaataa aaatggctaa
    14281 aaaaggtgga tttttattag gtgcagtagt cggaggaaca gccgcagcaa tcgccgcgtt
    14341 attgttagca ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc aagcggatga
    14401 tttgatggat atggcttcag attacacaga tatggcgaaa gaaaaatcga ctgagttaac
    14461 aggtattgca aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag
    14521 tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga agtgaatctg acgatatctt
    14581 gttaaatctt aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga
    14641 tatgaaaaaa cctgcagatg aattaggaag tattgctaaa gatacttcag aagatatcat
    14701 gattgatgta aaagacgctg ctggtaaagc aggagatact gtcaatgaag gaatgtcaga
    14761 agcaaaacca gttgcagatg acacaaaaga aatgatgaaa atggcaaaag aagatgcgaa
    14821 agaagctaaa aaagaaataa aagatacaat gacagattca tctacaaatc actaaaatca
    14881 caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta ctcttatttt
    14941 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacagccatc gcttgtcaca
    15001 tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    15061 gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga agggaattcg
    15121 tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    15181 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tggacggacg
    15241 gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt atattttttc ttggaagtcg
    15301 gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt ccaaacaacg
    15361 ccaagatcga ataacacaag ttcattcggt gaaatcttcg tgtttcctgg tgtaccatga
    15421 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    15481 tgttttttca attggtattc gatttctgca atgatcgctt gttcagtcac gccttctttc
    15541 actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc gatcagtttg
    15601 tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagt tagatcagtg
    15661 taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac agtcaaggcg
    15721 ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc ttttccgatg
    15781 atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca actactgttt
    15841 tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc aagagggata
    15901 aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta agcgatgtgg
    15961 ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg acgtagttca
    16021 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    16081 tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta aaaattttca
    16141 tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta ttttagaaat
    16201 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    16261 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    16321 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    16381 cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac
    16441 aacagttggt gtaatcatcc ctgatgtcag caatatgttc tttgcttctt tagcacgtgg
    16501 tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt cagatggcaa
    16561 cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg acggcgtgat
    16621 cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc
    16681 tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    16741 tgtatccgca acaaaagatg cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc
    16801 ttttgtcagt ggtgcgttga tcgatcccat caatggacaa aatcgtctga aaggctataa
    16861 agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg aagcacaata
    16921 caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg caacagctgt
    16981 ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag accgtggcgt
    17041 gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga cagaagtagc
    17101 tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg tatctatgcg
    17161 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    17221 tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag aggttgtgtg
    17281 cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcatct catgtttgat
    17341 gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttatttg ggaaaagggg
    17401 ttatgacaaa attttgttat aatccttttt ttgtgtctta aaaaagagag aaagtggttg
    17461 aaattatatt tatcgtatat aaaacaatcc gtgtatagat gctgtatttt gttcaccatc
    17521 atcaaaaaaa taagggaatg tgacaatact attgtcacat tcccttatta ctgtgtattc
    17581 tattaaatca atcatctttc tcatctgatt ctttggttgc ttgattcgct cttgcataag
    17641 ctgaagcggt gttccagtaa tctgtatagt tttcatcggt accgccaacg ccaaacttaa
    17701 aggcgctttt cttcggtcca tttttcgccc atagcgattc aactgttttt cctggtgttt
    17761 ggatggattt tttattgaca gtaacttttc caggtgtttg accagtaaat ccagatactt
    17821 ttttcttcat cacatcagat gataattcga atttctcatc agtgccaaaa atgtcaggtt
    17881 ttgcttggta agcttgattg atcagattgg caagataatt agccgaacgt atccttgctt
    17941 tgctgtccat tccagtattg tcatcgtgcc ctgcccagct actgatggta atagacggag
    18001 tagagacaat tagccatgaa tctcggtatt cattggtaga accagttttt ccgacccaat
    18061 ctgcttttcc tagatttccg tttaagcttg agatggcatc tttgaatggt gtagtgattt
    18121 ttgcattgat cacactgcgc atcatgtcat tcatgatcga tgccgttgct tccgaataga
    18181 tccggacaga gttgctttcg tgtttataaa ttacatttcc tgcgttatct gtgatggaat
    18241 cgatgatata gccttcttca tagacgccgc cgtttgctaa agcctgaaat ccgttggtct
    18301 gttgaagcgt cgtgacatct actgtaccta aaggagcaga ttcaacgccc caattgtcat
    18361 ttgccgggta gttcatcttt gacaagtagt gctcgtaagc atattgtttc gatccaccgg
    18421 aattcaacac atcttgatat aagtgataag ccgggatatt gttggaccat tctagtgact
    18481 cacggacggt ttggaaggtg ttcgatcctt cgttagtagc attgacgatt ttctcgccag
    18541 cattttctcc ttcttgccaa gttgttgcat aatctgatac acgggattct gatccgataa
    18601 gccccatgtc gattgcaggt ccgtaaacaa gaactggttt gattgatgag ccagcttgac
    18661 gttctgtatc gaaggcatgg ttattttgat tctgactgta attccgtccg ccaacgaatc
    18721 cgtagatgcg gccagttctg ttatccatca atacatttcc ggtttctact tgactgccag
    18781 caccatcatc taacatgtat ccataatttt caacgccatt ttgcaatgca gcatagacat
    18841 ctttatcgat tgtcgaatgg attgtatatc ctttattttg gatcgtttgc tgtgcctttt
    18901 gataataagc gtcactgatc gatgaatcag acatatcagc gttgtctttt tcagccaatt
    18961 gttgtgcaat gattcttgtt gcttcattca tcacagtata atacaagaat tctctgtcag
    19021 tctgttctgc gatttgttgt ggtagaaagt cttttgtaag gtcataggct tttgcttctt
    19081 cgtattgttc tttcgtaatt tggccttcac gatacatact gaataaaaca aaatcttttc
    19141 tggcaaggcc ggcagaaaga tcttctttta gcgcacctgt gttcgtgtat ggactatacg
    19201 tgatcgggct ttggggtaat ccagcgatat aagcagcttg cggcagagta acatcttttg
    19261 catctacacc aaagattcct cttgcagctt cctggactcc agcaatgttt tgtcccttat
    19321 tattacgacc aaatggtgag acattcaaat acgtagcaat gatctcatct tttgaaaagt
    19381 atttttctac ttgtgcagaa agaatgatct cgtttgcttt acgtttgaat gttgtttcat
    19441 ctgtaaggat ctgctgtttg actaattgct gtgtcaaagt tgatccacca gaagagccaa
    19501 taccagtagc ttcagatact aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt
    19561 tatcgaagta ttcatcctct gtgctgataa ttgcagtttt caataaaggt gaaattttat
    19621 cagatgaaat agtcgtgcgc atcagatctg tctggatttt tgatatctct gtattatctg
    19681 cataaacaat ctttgacgtt tctgtaatgt ttcctagttc tgtctgcagt gcttttttag
    19741 tcggcaattg tgtgtcttcg acgagatagg caaaatagcc agcgcctatt ccaataccta
    19801 gagctgcgaa catcaaaaga agaacagtaa taaaaacagt caatgattga aaaactctaa
    19861 aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt tttcgagtat
    19921 ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt atagcaaata
    19981 atagaacaaa aaaacagaca aggctgtttt tggcaggaat ttctttttta aaggagttaa
    20041 aacgtgagaa aatgatcttt tttataaaag agttcttaag cttttcttta gagaaaattt
    20101 cagttactat ggagaacagg gcaaagaaaa atcaatcatt ttctttgtat atcatcttac
    20161 caatatatag gaattgttag taacaattgc ctttcaagga ggaatccatc catgttatta
    20221 ggctcacacg tcagcatgag cggaaagaaa atgctgttag gagcagcaga agaagcagcc
    20281 agttatggat cttcgacttt tatgatctat acaggcgccc ctcaaaacac tagaagaaaa
    20341 gccatcgaag agatgaatat tgatgccgga aaagcgtaca tggcagaaaa cggtctaaca
    20401 aatatcgtcg ttcatgcccc ttatatcatt aatttgggga atacgatcaa accagaaatg
    20461 tttccttttg ccgtccaatt tctgagagaa gagattgttc gtgcggaagc tttaggcgct
    20521 actcaaatta cacttcaccc aggtgctcat gtaggggcag gaacagcagc cggaattgct
    20581 agaattattg aaggcttgaa tgaagttttg acaaaagaac agacggcaca aatcgctttg
    20641 gagacgatgg caggtaaagg tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc
    20701 atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgacacctg tcatacgaat
    20761 gatgccggtt ataatattaa agaagacttt tcaggtgtaa tggaagagtt tgataagatc
    20821 atcgggttag atcggcttaa agtactccat atcaatgatt ctaaaaatcc gcaaggttcg
    20881 cataaagacc ggcacgccaa tattggtttt ggaacaatcg gattcgacac gttgaataag
    20941 atcgtccatg atccaatgtt tgacaatatt tcgaaaatct tggaaactcc ctatgtcggc
    21001 aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa
    21061 acattcgatc cagaagtgtt tcctgagtta ttagcagaag aagtgatact ctaagatttt
    21121 ctatttgaca gcagcaaaaa aggaccaggc acattgcctt gtcctttttt gtttaagaaa
    21181 caaaggagta ctttttgcag tctttttcat catgcatctt ctcccccctc tttttaaatt
    21241 tagacaaaca gggtatactg gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg
    21301 caaaacgtga ttggaaggac gaaaagatgc taaacacttt aaaacccttg atcaaccaac
    21361 tgactaagaa acatacatac tttattcttg gcatagccct attatttaca attattcgtt
    21421 tgtatttata tagtaaagca atctatggaa cagacatgtc ggcaacctat gatgatcagt
    21481 tgctgattcg ctatgctcaa aatattttgg acggtaaatg gctaggggag tatacgacta
    21541 caacactttc caaaggaatc tcctacagct tatttttagt acttgcacat aaactgcatt
    21601 tttcttatgc agttttattc ggcatgttca gtatttttgc cagtatattg gttgcactcg
    21661 cgtttaaacc gatcgtgaag aacaagtatc ttcttttaag tatctatttt tattttttgt
    21721 tttctccgat tagttttaca catgaatatt ccactcgaac gtatcgtaat gccctcgtga
    21781 ttccggctgt tgagatcatt atcgcttgtt tattagctat ttattaccgc aaatacggtt
    21841 ctagcagaaa attgctgcca tggtttttag ggctttctgt cgcttttcct tttttttggt
    21901 tcattcgcga agattctatc tggcttcttc cttttactgt agtagctttg ggtatcagtt
    21961 tcgtccagat cttgctaggg caagactttt ctttcaaaga agtaaagaag ttaaaaaaac
    22021 gactctttct tctctcgaaa cgggaatggc tgaaactttc attgttttta cttcctatag
    22081 ttggcttttc catcgtctat cagtggatcg aatataaaaa tgagacaaca tacggtattg
    22141 caaccatcaa tgaccgttcg agtggagaat ttggacgttt gatgaaacaa ctgatccgaa
    22201 tagatgatga aacgaacttg aataaagaaa acagtgagat atgggtctca catgctgcac
    22261 taaataaagc attagccgca tctcctacat tccatactgt ttctacccgt attgacgaat
    22321 tatatacgac tcacgcgtgg actcaaggtg gaaaagtaaa agaattaccc ggagatatca
    22381 tcttttgggc attgcgagat gtagtaagcg aatctggtta ttaccaaaac aatgcaaaag
    22441 aaaccaatga tttttggaaa aaagtcaacc aagaattaga agaagcctat cacaagggaa
    22501 ccatcaaaaa gaaaaaagag ttctatctga tgggatcagg agatggaaag acgatgagtg
    22561 atctgccaat cgtattcgat ttccttcaat caggatacgc ttatgcatgg cgatataaag
    22621 atttccatca aggaggaaat tatagtttcg gaacgcaaga acaagtcaaa caggctcaag
    22681 aattattaaa ccttccgcta atggagaatt ggatcgatag taatgaaccg agaacaccta
    22741 acttccaatt aacaaaatca gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc
    22801 tggctattcc cgtgctgatt gcttcaatag cagggatatt acttatgata ctaggaaccg
    22861 tatttgcaaa agctgatcgt gcctattata gttcctcttt gattgtggtc accggattaa
    22921 tactgacaca attggttttt cttttcggtg tctcatggtt ttgttcttat gcgccagaaa
    22981 aaaaggacta ttttttaagt gtctacactg gtgcaggcgt gccattaacg caattcatcg
    23041 tagtaacaag catgctagtt ttcagtaaat taaatctgaa aaaagcttta tcttctaaat
    23101 aaaaagaccc ttggtagcta gatttttaac ctaactatca agggtctttg tgtggaaaaa
    23161 tcagttttta ttcgatttct tctaccgttt cctgcaaaag ctgttcatat tttttgtttc
    23221 ctttatataa atcaccaatc gtccatgcca acaggagaag gacaagaaca atgattacat
    23281 aagcgatgtg atcagccaat gccagttgag cggaagtggc ttgctcgccg aagaaacttt
    23341 cgatctgtcc tggtaatcct cgtaagttca agtaagttag actaatcaca gagaaccagc
    23401 caagaagttt tacgatgaat gaatttttaa atcggctacc catctccgct tggctgttgg
    23461 tcatcatcag taaaggaatc atcgaaaatg gcaacgcaaa agccaagaac acctgggatt
    23521 cattcattaa gttattcaaa gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca
    23581 aaacacaaat caatacagga atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa
    23641 tcgggatacg catatggatg aatccttcca tgatgacttg accggtcaac gtaccagtaa
    23701 tagtagagtt ctggccagaa gcaagaagtg ctacagcaaa taaagtggac aagataccag
    23761 attttgctac agcaatcaaa ataccattgc tcatagctga tggatcagat aaagcttcat
    23821 acagaccaaa aaaggaagga tcttggacag aaccggtttt gaagacagcg acacccatga
    23881 tcaatagcag tgcattgact agaaaagcca ttgacaactg gatattagaa tcccaagtag
    23941 aaaaacgtac agcttgtgct acatcttctt cgttttggtg atcgatcttc cgagtttgtg
    24001 aaactgctga atgaagatat aagttatgcg gcatgactgt tgcaccaata atccctaaag
    24061 ctccagttag cggagtttcg ccaccaatga caggtgagtc tgagaatgta tggctgttag
    24121 ggataaagcc agcaaataaa ctgtgccaat ctgggttaga caagccaact tgatagacaa
    24181 aaacgaataa aatgacgaaa attaagcaga caaccagtgc ttcgatctta cgaaacccga
    24241 cttttgtcag tagtaagaga acgaaaacat ctaatacagt aataaagacg gcaatcacca
    24301 atggaatatc aaaaagtaag tagagggcga ttgctgcacc aattacttct gcaatatctg
    24361 ttgccataat ggctaattca gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc
    24421 gtgctcggat tgcctgtgcc aaatccattt ggctcacgat tcctaatttt gctgccatat
    24481 actgtaaaag catagcaata agactagaca tcaaaataac tgacatcaat aaatattgga
    24541 agttttgtcc tccggtgatc gaagtagacc agttacccgg atccatatac ccaactgcta
    24601 ctaacgcacc aggtccagag taagcaagaa gtgttttaaa aaaacttttc ccctttggta
    24661 catcaactgt tccatttact tcagctaaag ataaaccatt tgtatgttca ataagtcgat
    24721 gcttatgaaa ttgtttattg gattgattat tcaagaaaaa accacctttc ctatttatct
    24781 acgattacat tctacacctc aaaaagaaaa aattaaagaa aaagattcgg tatgcctaat
    24841 ttattttcgt ggcgtgtctt gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac
    24901 cgctattttt tgcatgaaac taaagaatac gcctttcttt tttcaataaa ctttagtaaa
    24961 ataagtcatt gagagcaatt cggaaataga aaggagagat ttatatgtta tcaacaggta
    25021 ttgtcgtact tattgctatt attgctttgc taatcggcgc agtaggtggc tttttcctag
    25081 cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc cgttaatgag gacatgttgc
    25141 gatcaatgat gatgtcaatg gggcaaaaac catctgagaa gaaaattcgc cagatgatgc
    25201 agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca acgacttttt acgggctgat
    25261 tttttcagcc cgttttttat ttcgtaattt gttagactat tcagattatt cagcaaaaaa
    25321 gacaagcaca gacaatccta ggctcttgat aaagacaaag gaggagtaaa atgtcgattt
    25381 ttagaaaatt aggctggttt tttcgtcagg aaaagaaaca ttatttaatt ggagttttct
    25441 cgctattcgc agtggccttg gtacagttag ttcctccaaa agtgattggg atcatcattg
    25501 atgagattgc cgataaaaat ttatcaataa agatcatttt cgggtggatc ttcctattga
    25561 ttatcgctgc ttttttacaa tatctttttc gttatatctg gcggacgaat atttggggaa
    25621 gtgcagctcg gttggagaaa gaacttcggc agcagttatt caaacatttt acagaaatgg
    25681 acaatctctt ttaccaaaaa tatcggactg gggatttgat ggctcatgca acgaatgatt
    25741 tgaatgcgat ccagaatgta gcaggagctg gtatcttgac ctttgcagat tcagtgatta
    25801 cgggcggaac aacgatcatt gcgatgattt tgtttattga ttggcgctta acactgattg
    25861 cgatgattcc tctgcctttg ctggccatca cctcacgagt gctgggatcg aaactccatg
    25921 atgcttttcg tgattcgcaa gaagcttttt ctagtatcaa tgataagaca caagaaagta
    25981 tcactgggat caaagtcatt aaaacatttg gacaagaaaa agaagatcta gcggattttg
    26041 aagaaaagat tgaagatgca attattaaaa acaaacgagt caatttttta gactccttat
    26101 ttgatccatt cattaccttg attatcggtc tgtcttatgt tgcaacaatt attttaggtg
    26161 gaaagtatgt cttggaaggc acgatctcga tcgggcagat ggtctcattt attagttata
    26221 tcggtatgct tgtttggccg atgtttgcga ttggtcgatt gttcaacgta ttggaaagag
    26281 gaaatgcttc ttatgatcgc gtcaatgaac ttttgcatga aaaaacgcat atcgtggaac
    26341 aactgggtgc agtgcagacc ccggcaaaag gcgagctgaa tgtagctatt tcctctttta
    26401 attatctgaa agaagaatca ccagcattag aacaaatcaa gtttgcaatc caagaaggag
    26461 aaacattagg tattgttggg aaaactggtg cgggaaaaac gacgatcatc aaattactga
    26521 tgcgtgaata cgatgattat caagggaaaa tccaatttgg cggacgttcg atcaaagaat
    26581 attcattgaa tgctttatta ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt
    26641 cgatgaccat tcgagataat atccgttttt ctaatccaaa actttcagaa gaagaagtaa
    26701 agcatgcggc ttctttagca tttatcaaca atgaaatcaa ggaattacca aaaggttatg
    26761 ataccttagt tggagaacgt ggagtctctt tatctggcgg acagaaacaa aggatttcga
    26821 ttgctcgagc attgatcaaa aatcctgaat tattaatgtt agatgatgca ctttctgcag
    26881 tagatgccaa aacagaagaa gctatcttgc ataatttaaa agaaataaga aaagagaaaa
    26941 caactatcat tgcagctcac cgtttaacag aattaggcag aagtcgccca tcctctatag
    27001 gtgggtgatg aatgccgttc ggtatgaggg ttaccgttgg taactcgaag accgacaact
    27061 aaggcgtatt gcctttttat tttgt
//
LOCUS       DAJSVP010000026        25855 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSVP010000026 DAJSVP010000000
VERSION     DAJSVP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.026"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            207..803
                     /locus_tag="ON449_001196"
     CDS             207..803
                     /locus_tag="ON449_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3933793.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(861..1343)
                     /locus_tag="ON449_001197"
     CDS             complement(861..1343)
                     /locus_tag="ON449_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933794.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1449..1646)
                     /locus_tag="ON449_001198"
     CDS             complement(1449..1646)
                     /locus_tag="ON449_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933795.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1941..2699
                     /locus_tag="ON449_001199"
     CDS             1941..2699
                     /locus_tag="ON449_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3933796.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2759..3187
                     /locus_tag="ON449_001200"
     CDS             2759..3187
                     /locus_tag="ON449_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933797.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3233..3305
                     /locus_tag="ON449_001201"
     tRNA            3233..3305
                     /locus_tag="ON449_001201"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3266..3268,aa:Gln,seq:ctg)
     gene            complement(3455..3826)
                     /locus_tag="ON449_001202"
     CDS             complement(3455..3826)
                     /locus_tag="ON449_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3933798.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4041..4226
                     /locus_tag="ON449_001203"
     CDS             4041..4226
                     /locus_tag="ON449_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933799.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4309..4497)
                     /locus_tag="ON449_001204"
     CDS             complement(4309..4497)
                     /locus_tag="ON449_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933800.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4521..4784)
                     /locus_tag="ON449_001205"
     CDS             complement(4521..4784)
                     /locus_tag="ON449_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933801.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4942..5199
                     /locus_tag="ON449_001206"
     CDS             4942..5199
                     /locus_tag="ON449_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3933802.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5270..5461)
                     /locus_tag="ON449_001207"
     CDS             complement(5270..5461)
                     /locus_tag="ON449_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933803.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5602..5922)
                     /locus_tag="ON449_001208"
     CDS             complement(5602..5922)
                     /locus_tag="ON449_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933804.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5945..6379)
                     /locus_tag="ON449_001209"
     CDS             complement(5945..6379)
                     /locus_tag="ON449_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3933805.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6714..7352
                     /locus_tag="ON449_001210"
     CDS             6714..7352
                     /locus_tag="ON449_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3933806.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7676..7759)
                     /locus_tag="ON449_001211"
     tRNA            complement(7676..7759)
                     /locus_tag="ON449_001211"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7723..7725),aa:Leu,seq:cag)
     gene            8287..8787
                     /locus_tag="ON449_001212"
     CDS             8287..8787
                     /locus_tag="ON449_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3933807.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8977..9321)
                     /locus_tag="ON449_001213"
     CDS             complement(8977..9321)
                     /locus_tag="ON449_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3933808.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9475..10395)
                     /locus_tag="ON449_001214"
     CDS             complement(9475..10395)
                     /locus_tag="ON449_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3933809.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10582..11073
                     /locus_tag="ON449_001215"
     CDS             10582..11073
                     /locus_tag="ON449_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3933810.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(11097..13472)
                     /locus_tag="ON449_001216"
     CDS             complement(11097..13472)
                     /locus_tag="ON449_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3933811.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13798..14424
                     /locus_tag="ON449_001217"
     CDS             13798..14424
                     /locus_tag="ON449_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3933812.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14447..15151
                     /locus_tag="ON449_001218"
     CDS             14447..15151
                     /locus_tag="ON449_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3933813.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15343..16176
                     /locus_tag="ON449_001219"
     CDS             15343..16176
                     /locus_tag="ON449_001219"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3933814.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            16373..18301
                     /locus_tag="ON449_001220"
     CDS             16373..18301
                     /locus_tag="ON449_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3933815.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            18439..18894
                     /locus_tag="ON449_001221"
     CDS             18439..18894
                     /locus_tag="ON449_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3933816.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            19364..19633
                     /locus_tag="ON449_001222"
     CDS             19364..19633
                     /locus_tag="ON449_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933817.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            20033..20287
                     /locus_tag="ON449_001223"
     CDS             20033..20287
                     /locus_tag="ON449_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3933818.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20376..21422)
                     /gene="holA"
                     /locus_tag="ON449_001224"
     CDS             complement(20376..21422)
                     /gene="holA"
                     /locus_tag="ON449_001224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3933819.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(21460..23733)
                     /locus_tag="ON449_001225"
     CDS             complement(21460..23733)
                     /locus_tag="ON449_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3933820.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(23726..24265)
                     /locus_tag="ON449_001226"
     CDS             complement(23726..24265)
                     /locus_tag="ON449_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3933821.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(24401..24619)
                     /locus_tag="ON449_001227"
     CDS             complement(24401..24619)
                     /locus_tag="ON449_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3933822.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(24616..24861)
                     /locus_tag="ON449_001228"
     CDS             complement(24616..24861)
                     /locus_tag="ON449_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3933823.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(24977..25705)
                     /locus_tag="ON449_001229"
     CDS             complement(24977..25705)
                     /locus_tag="ON449_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3933824.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         7970 a         5024 c         4391 g         8470 t
ORIGIN      
        1 caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa aatccttaca
       61 taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt gagcatatct
      121 gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa aaaatgcaaa
      181 acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg aaaaaccgcc
      241 gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta acaactttga
      301 tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca cgcgcagtta
      361 tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca cttcgcccat
      421 tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc aaaaatggtt
      481 atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa gaacaatttg
      541 aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc gcaacagcta
      601 atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat tacaacccag
      661 ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa ctacgttcgc
      721 aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg aacgatgccg
      781 atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt gtgacaaaca
      841 tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc tgcctcatcg
      901 tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa tttcagcatc
      961 ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc ttttggctga
     1021 tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa gctattcaaa
     1081 acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa aatcattgct
     1141 gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt ataccaaatg
     1201 actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac agtccgataa
     1261 ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa taaaaacaat
     1321 agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc agaaaatagg
     1381 aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg cacgtccaat
     1441 tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt ttcatctaat
     1501 aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt tgtcattttt
     1561 gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc atattgttct
     1621 cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa cttttctcca
     1681 cattaccact gctatttctt tggagattta actcattgat ttttatttac agttaaaaat
     1741 caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca gctgttgtat
     1801 ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt atttcctaca
     1861 tattaaaata taatacttcc atttccaata aaaatacatt tataatagga aagataaaca
     1921 gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg tttctagggg
     1981 gcacagctgc tcttttatta agcggtacaa gttattacct acatatccat tctccagaac
     2041 atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca aaagagaaag
     2101 cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct ttagaaaatg
     2161 gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa acaactaaaa
     2221 accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg actcatggcg
     2281 atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg gtcgtctatg
     2341 ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac acttttccaa
     2401 aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat cctgtctgga
     2461 cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact tggaccacaa
     2521 ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact ggatatgaca
     2581 aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta ccgatcaacg
     2641 atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag aaaagctagt
     2701 aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag cgtagataat
     2761 ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt ttgtccgtga
     2821 ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc gtcgcagacc
     2881 tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt attacgcaga
     2941 tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa cagagtatac
     3001 tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg aacctgtcaa
     3061 agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc ttcctgctgg
     3121 attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat ccgatcaaga
     3181 aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata aatgggatgt
     3241 agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga accctgccat
     3301 cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat ctttttgcag
     3361 ggggattatt gtattatttt tactacttgt atttagttta cataattaaa ttatgaataa
     3421 taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct aatgctttct
     3481 ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct ttattgctaa
     3541 ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc gttcgttctt
     3601 tcccattggc atcagcagct ttctgcacat accttgctgt accatattca tttacttcct
     3661 ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt agctgatcaa
     3721 gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca aatagcaaaa
     3781 taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt ctacctcctt
     3841 gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag ttggagattt
     3901 ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc gttatttgat
     3961 attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa atcatgttga
     4021 tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc aaggaaatga
     4081 ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa gaacaattag
     4141 gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac agtgtcaaaa
     4201 acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat caaaggagga
     4261 caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact aaaataaacg
     4321 tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca ccacatattt
     4381 acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc tttttacatc
     4441 aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg gattcatttt
     4501 tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc ttttttggta
     4561 tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg gatactataa
     4621 taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat accatttatt
     4681 ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat cacccgaata
     4741 ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat cccactcctt
     4801 ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca gattttaccg
     4861 gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt atcatggact
     4921 ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta tattcctttt
     4981 tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa gttttcagtt
     5041 ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt taggcattgg
     5101 gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt aatttttctt
     5161 attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat gtttaaaaag
     5221 aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc tattctgtta
     5281 taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct ataaattctt
     5341 gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg cttttcgctt
     5401 cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt cctgcttcca
     5461 tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat acgcattatt
     5521 ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc agttttctaa
     5581 aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt ctgttaattt
     5641 atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata attccataaa
     5701 tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag ggaatccttt
     5761 atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt tccactttac
     5821 taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg ctaaaatttc
     5881 tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt cctcccttat
     5941 ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa gaagcggtcg
     6001 atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg atcaaatcaa
     6061 tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca gtttcaacta
     6121 ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc agcatggcta
     6181 cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca ctatgggtca
     6241 gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc tttgctaagt
     6301 caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct accatgatgt
     6361 tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac atctcaagca
     6421 aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca atttctttca
     6481 agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt atttctgcat
     6541 tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta caaaaaacgt
     6601 ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg tgttaaagta
     6661 ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt ttcatgacat
     6721 cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc ttcatgggaa
     6781 cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta tcaacgattt
     6841 ctcaaaaata cgttggtgat aactccttga tcaatgccat tgcagaatca aattcaattt
     6901 ctgatattaa cctgatctac tcaggacaac aattaacaat cccaacagaa ggctctgcac
     6961 aagcagctgc tgaacctcaa gcagccgttc aagaagcacc agtccaagct gaacctgttc
     7021 aagctgaaca acctgttgta caagaaacgg ttcaaacgga aacacaagca gcgcctgttg
     7081 ctgaaacaca acctgctcct gctgtaacag aaactgctgc tactccagca agtacaagct
     7141 ctgcaaaaga atggatcgca caaaaagaat caagcggttc ttacacagca acgaacggac
     7201 gttacatcgg ccgttaccaa ttagattctt cttacttaaa tggtgactac tctgctgcaa
     7261 accaagaaag agtagcagaa caatatgtta cttcacgtta tggttcttgg gaagcggcga
     7321 aagcattctg ggaagcaaac ggttggtact aagattcatt atttgaacgt ttaaacacat
     7381 tcaaaatata gtaagaggtc atgcgattta ttctttcgca tgacctcttt ttgagtttaa
     7441 gtttagtctt tattccttaa ctatcgtttg ccagtatttc ttgtatcaga atctataaat
     7501 ctactatttg aatcaatcta ttaggtttta aactgtccca ccttcacaca aatttttttt
     7561 gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt agataatatg
     7621 ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca aattatgcgg
     7681 ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct agcgcgtctg
     7741 ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt tttttgtcaa
     7801 gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc tacacaaata
     7861 attcatccga gcaactccat catctattga gggttggtga aaaaatctgt tttatccata
     7921 tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta tttttttctt
     7981 catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga tattgattat
     8041 ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc acgttttctt
     8101 tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt attgtaattc
     8161 atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa aataggcatg
     8221 gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact taaagaggtg
     8281 aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta tcagctacaa
     8341 actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta tacatttgct
     8401 gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag
     8461 agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta tgtaggaatt
     8521 tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca tattggttac
     8581 ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc attagtacta
     8641 caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa aaaaaatctt
     8701 ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga tgagaaagaa
     8761 tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa gggatttgaa
     8821 tgtcctttat caatagaaca catttattta attaacttat aatatagcag acaaaatttt
     8881 ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct tttttgtttt
     8941 tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca acgcaatccg
     9001 tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt tgacttctgc
     9061 tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta aaatatctgc
     9121 taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat tttcaggatt
     9181 gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt caggatcaat
     9241 caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt gtccagaccg
     9301 ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat ttatcatact
     9361 ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta aatttataaa
     9421 aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga ttcctcattt
     9481 tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca ttttttgttc
     9541 gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga taaatgcatc
     9601 gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg tgatgaccat
     9661 catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta atgtcattcc
     9721 acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg taacgatcat
     9781 tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca ttgcataaat
     9841 ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga tgactacaat
     9901 gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt gtgaaagtac
     9961 aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata atcctacgcc
    10021 aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag tgatctgtcc
    10081 gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac taaggatcgt
    10141 tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca aaagaaaaat
    10201 aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg aaatgatttg
    10261 agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat aatttaaagc
    10321 gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc tttcattttt
    10381 atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt attccaaatg
    10441 taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag aagaatttta
    10501 gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt aaatatttat
    10561 atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt atcagaagtc
    10621 aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc caatcgcttt
    10681 gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt tgtattgagc
    10741 gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct gatcaaaaga
    10801 aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg tggcaaagct
    10861 cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa aatcactaca
    10921 gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca gattagagtg
    10981 acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa acttgactta
    11041 ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga cgtctgtcat
    11101 tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat tccttcttcc
    11161 aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa taaacgtcga
    11221 taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc cgccatgcca
    11281 cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg ataagcgtaa
    11341 gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg ctcatgattc
    11401 atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc tctcaaatcg
    11461 attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc agcgggattt
    11521 gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt ttcttggatt
    11581 agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc taaatattga
    11641 taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc atgaatcgtc
    11701 tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag gtaatttgcg
    11761 atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc cagacttttt
    11821 tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga aaaaagaata
    11881 ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat acaaaactct
    11941 tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc atcataaaat
    12001 tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg ttcttgtgat
    12061 gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg ataacctgcc
    12121 tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc ttcttttgct
    12181 atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg aagttttttg
    12241 aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag ttctgccgaa
    12301 tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa cagatagtcc
    12361 tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa taaataattg
    12421 taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc gaaaggacaa
    12481 agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt ctcatgattc
    12541 aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata tgggcactga
    12601 tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt caaagtgaca
    12661 ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc tacttgtcta
    12721 gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc tacagctttc
    12781 aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc tacaaacaat
    12841 ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg acctttccta
    12901 tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt gcgccagttt
    12961 tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa agtttctaac
    13021 tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt ctggaagtaa
    13081 gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata ggcatacacc
    13141 cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa tcgtggttcg
    13201 gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc gtctgcacgg
    13261 ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa gacttctttt
    13321 tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc ttctaatgca
    13381 gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa ttctactaag
    13441 tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc tctaagatta
    13501 cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa aacaccctgc
    13561 tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta atgtaacaag
    13621 gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc aaatactttt
    13681 catatagtct gataattcag ataagtaaag agaaataagt acctgttttt gtttcctagc
    13741 aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg aatgcacatg
    13801 catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt ctacaacgaa
    13861 gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa
    13921 aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga tcatatcggt
    13981 gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc actagagcaa
    14041 aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga tgatattgct
    14101 gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac tttaggaaac
    14161 atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag ctttattttt
    14221 gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag aaccgatctg
    14281 tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt tgtattacct
    14341 gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca ggaaaaaagg
    14401 acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga cagaaactac
    14461 actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag tacaaggctg
    14521 gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag gtcgcgggtt
    14581 aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc
    14641 ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta ttgaaaaaag
    14701 gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa tgtggggtgt
    14761 gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag aagtttcttt
    14821 tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg aaacttatga
    14881 aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa
    14941 tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt tgcttaatct
    15001 gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac
    15061 ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata tcgataacgg
    15121 gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt accagagaat
    15181 cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt gtacttatct
    15241 gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc aaaaaataac
    15301 ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt tgcaaatatt
    15361 atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc catcagtagt
    15421 actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc acaatttatg
    15481 tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt
    15541 cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca
    15601 ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt gcctttggat
    15661 gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt gatcgtttac
    15721 ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct
    15781 ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt cttggcactt
    15841 gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat tggtgcttcg
    15901 cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc
    15961 ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga agaaactgtt
    16021 gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat caaattctta
    16081 ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat tatccttggt
    16141 atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca cttctcttat
    16201 ttgagaaatg ttctggtttt ttattttctt taataataat cattataaat aataaaaaca
    16261 acatcaagct taatcaccaa atgataacca ttatcgttct catttagctg ttattattat
    16321 caattacatt tttattatgc tatccttgtt ttgacaagga ggtctaataa cgatgactaa
    16381 tcagaaaaaa gcttttgcag caacaatcgg ctgcctttta tttatgatct tcggttttat
    16441 cttcgaccga ttctctctta ccatcacccc acttttttat atcagtgcca ttatcatcgg
    16501 tggatataaa caaacatcgg aaggcttgaa agaattgtgg aatgatcgga cactgaatgt
    16561 ggatttattg atggctttag ctgctattgg tgcctgtttg atcggtaatt actttgaagg
    16621 agcaatgttg acattcatct tttgtttaag cggtgcatta gaggaataca caactaataa
    16681 aagtcaaaaa gaaattactg ctttgatgaa tcttcaacca caaaaagccc aattattaaa
    16741 agaaaatgga caaatgcaag aagtagatgt ttctcaacta aaggtggata acttggtatt
    16801 tgtcgctaaa ggagcagctg ttccgataga tggcttttta gaaagcacac aggcaacgat
    16861 cgatgagtct gcattaagcg gtgaatctgt gcctgtagaa aaatatttag gcgatgagat
    16921 gtatgcagga acgctgaatc aaggaaatcc catcacgatt cgagtaacaa aaacgagttc
    16981 cgaaactgtc tttgctaaga ttatccaatt agtagaagaa gcgcagaata cgccaactca
    17041 aacagcttca tttatcgaac gaatcgaaaa taattatgta aaattgattt tgcttgctgt
    17101 tcctctaatg atccttctcc cccacttttt cctaggttgg agttgggatg aaagctttta
    17161 tcgaggaatg gtattattag ttgtggcttc tccttgtgcg cttgtcgcat ctgctacacc
    17221 agcttctcta gctgctcttt caaattctgc aaaaaatggg attttaatca aaggcggtat
    17281 ccatttagaa aagctttctg aattaaaagc gattgccttt gataaaacag gaacgctcac
    17341 taaaggaaaa ccagttgtaa ctgacagctt tttctttgat gaaaaggatt tggcacaaca
    17401 acttcttgtt gctatggaac aaaaaacgac gcatccactt gctcatgcga ttttgcaata
    17461 tttcgattgc acaatacctg aagaaatcca atacttacca atagaagaaa ttactggttt
    17521 tggattacaa actacctatt taggtgcgca atggaaagtt ggcaagcacg catatgatcc
    17581 tgagacgatg ataatcagca aagagattgc ggaaatgatc gaacgtttgg aaaatcaagg
    17641 caaaaccgtg atttatcttt ctaaagatca gcagttaatt gctgttttag gtctgctaga
    17701 tattccaaaa gcaaataccc aacaagtaat ttcctacttt aaatcccaaa acatccatac
    17761 aagtatgata accggcgacc attctggcac agcaaaggcc attgctgaac aagtagggat
    17821 tgaacattac tatgcaagct gtactccgga agaaaaaaca caactagtca aaaaagagaa
    17881 tgaactttac caagtaaatg cgatggttgg tgatggagtc aatgatgcgc ctgctttagc
    17941 agccgcttct ttaggtgtcg ctatggggca agggacagat gccgctatgg atatcgcaga
    18001 catcgtctta atgaaaagcg acttaaataa atttgtctac agtcatcggc tatctttaaa
    18061 aatgcggcgg atcatcaaac aaaatattat tttttcgatt cttgttattc tcttgttgat
    18121 tgcttctaac ttcctgcaat ttttaaatct acctcttgga gttgtaggac acgaaggaag
    18181 cacaatattg gttattctga atggcttgcg tttactaaga cctttacctg atctttctga
    18241 ttcttcaaaa aaatgtaagt cttgtccact atacaaagcg cggctgtctt ctgtcaaata
    18301 agcaaagtgt ttttttatga ttttgtttat tttatgaaaa aaacagctta tgaataggtc
    18361 ttgaaggatg aattcctact tttgtaactt tctaaaaaca tgttatgatt gtttcgaaga
    18421 aagtgaggaa tatatttcat ggaaagttgg ctttttttag gaattatttt acttgtagca
    18481 attttagcta aaaatcaaag tctgatgata gcggcagctt ttgttttgat tatgaagctc
    18541 ataccagcct cccgttcctt tttcccttgg cttcaaggca aaggaatcaa tattggggtg
    18601 acgatcatca cagcggctat tctaatccct atcgcaacag gagaaatcgg tctacgtgat
    18661 ctattagaag cctttaaatc ccctattggc tgggtagctg ttttttgcgg tgggctagtg
    18721 gctgtcttat cttccaaggg tgtaggattg attgcttctg atccacaaat cactgttgca
    18781 cttgttttcg gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc cggaccggtc
    18841 attgcttctg gtatcactta ctgtatctta caagtatttg ctttaggaaa atagaacaaa
    18901 ttttgtctat tttaggagat tcttaacacc cccaatttac tgatccgtga tatactgaca
    18961 acgaggtgat acaatgttcg aaaaattaaa gtatttcata ggaacaatcg gctggtttct
    19021 tctagcggta cttttgattc tgggcttagc ttatctatta tttcctgatt tgatacaaaa
    19081 tgtgtttgat actgcttttt gattgaccta ttaaaacgat ataggggatg ctcctgcgat
    19141 gcaggggctt tttttaatca atgttttgta ataatttagg ccgtgccaat gagacttcct
    19201 atagaattcg atgctctttt ttctttttgg ctcttctgct cttttttgtt atactagatt
    19261 acagtggaca aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa tggaagaatt
    19321 agtgcctgtt ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa cttttgaagg
    19381 tcttttaatt gatacgtacg ttttacaaga acagccaatg atgctgactg ggatcaactt
    19441 gcagcttgag gaccgtgtga tcaatggcct gcttaaagat cgctatttgg caaaatatat
    19501 cttacgtttg cccctaaata aatatgtgct ggaagtaagt ggcaaatgga accgtcggaa
    19561 ccagttggtc ataaaaaaaa tgttgataaa aaatatcgat gaatacatta aaataatcgg
    19621 tacaccagaa tagcctttac tgatcaaagg atactggtta taaaataact gcagtttctt
    19681 aatagaaaaa caaagaacaa cagggaatcg tatccctttt caaaaaaaac tttgcaaacg
    19741 gttctctttg ttatataata acttagtacc aaacattccc attcaaactt tgtttaaata
    19801 gataaataaa aatctagttt ggtttgacgc tattgcctcc cacaatagcg tctttttgat
    19861 ttttttcagc ttgtactatc cgtttttact gaaaaaaaat cctaataaaa gactgttaag
    19921 gaaaaaaact tgtcatgttc atattgcaaa gcacgctgcc tttaggtata ataatgaatg
    19981 ttgaattatg cggtaacacg cgtaagaact taagttatcg gaggtgaaac taatgccaaa
    20041 cattgaatct gcaatcaaac gtgttcgtac aagcgaaaac gctaatgtga aaaattcatc
    20101 tcaaacaagc gctatgcgta ctgcaatcaa aaaatttgaa gatgctgtag cttcaggtgc
    20161 tgacaacgta gatgcgttat ataaagaagc tgttaaagct attgatatgg ctgaatcaaa
    20221 aggtctaatc cacaaaaaca aagctaaccg cgacaaatct cgtttaagca aaaaaatcgc
    20281 taaataaaag aatcggcttc ggccgatttt tttatttgtc aaaaaaagga caaaaacaaa
    20341 cgaccttccc gcatgctttg ccctcgtctt tcttcttatc gatttcttaa caactgccct
    20401 gtgcgcaaaa cgaatagttg aaaacttaat tctttttcga tttgtcccgt tttgatttga
    20461 taatccatct caactaattg atcaaacaac tgtaccagca aatgttcatc gaattttttt
    20521 acttcctgca tggcaagttt tacacgatat ggatggattt tcaatgtttc agcaatattt
    20581 gcctgctgat aaccaatctt tacaagaaat tttgtctgta aaaataaacg taattgagaa
    20641 agcaaaatcg cattaatttt gattgtttct tccccttgca taactaaatc ttcgtataaa
    20701 cgcaaagctt gttccgtctt cccttttaaa atatattggg tcatgtcaaa aatattatgt
    20761 tctaacgttt taggaactaa ttcttctact tctcttaccg tgatttgctg ttgattttgt
    20821 ccataaagga ccaatttctg taactctctc atcattttgg ataaatctaa gtcggtcaac
    20881 ctcaagaata aatcaattgc acttcgttcc attttgattg aggaattctc taaggtattc
    20941 atcagatatt gacgtacttc tttttcattc aactgttgga cattgatgat aattgctgtt
    21001 tttttcaatt gtttcgttac ttttttccgt tcatccaact tttcatatgg cgcaaagaat
    21061 actaatacag tcgattctaa tggttctttc aaataagccg tcaataaatc agtattttgt
    21121 tcgattccat tgtttacttt ttcacctgtt aaaaaataag gattctctac gaatactaac
    21181 cgttgatctc cgaaaaacgg caacgtctct gcttcagcta ctacctcatc taaacttgca
    21241 ttttccatgt caaaacttaa aaaatttaag tcatcttcgc cctctaattg gatttttttg
    21301 acaatttctg tccgaacttg atcttggaga aacttttctg ttcctaaaat caagtaacaa
    21361 gatgcaagtt tttctttacg tattttttgt aactctgctt gtatactcaa attactcacc
    21421 actttcaaaa attatcctac catgagcaga tgagaattac tagtctatca ttgtttccac
    21481 tagcccatga ggatgaaaaa ctggatgcca gcgataggtg atcattcctt gttgatccgt
    21541 tcgccatgtt tttatctgat tttcttctag tgtctccaat acttcgctgt gcgggtgacc
    21601 ataacgatta tttacgccgc aggatataat cccatgtttt gcttcaatat gggaaagaaa
    21661 atctgtgtta cttgacgtac ggctaccgtg atgacctaat ttgactacat cggctttcag
    21721 attaggataa atcaaaagca attgtttttc cattgcttga ttcaaatctc ctaaaaataa
    21781 aaagcgccta ttgttatatt ccatccatag accaatcgaa tcttcatttc ttccctctcc
    21841 cttttcaaaa ggagtcaata cataaagatc ataataaccg ctgatcctgt ccccatgttt
    21901 gatttcaatg atctcagtac catttttttg agcttcagat aaatgccttt gtaatggctc
    21961 atttcgtcca gcgccccaac cgatataaat ttttttgata gtaaaatgct gagataacgt
    22021 gggtaattct cccacatgat ccgtatcgtc gtgtgtcaag atgagtttat cgattacact
    22081 tatcccctga ccttttagat atggaacgat atttctttca gcaggaggac gtatcatacc
    22141 ttgcgcccac ttttctcttt caaagtgtaa ctgacctcca gtatcgatca aaatcgtttc
    22201 ttttcgaaaa ggactttgca aaaagatact gtctccttga ccgacatcga taaaagcgat
    22261 cgttgaagaa aagcgtaaaa atggcaaagt caaaaaaata aaagaaaaaa gaccaacata
    22321 aaacaagtgt ttcacaggtt gttctttcaa cctatccata caaatcagta atccgaaaat
    22381 atacatccca gatagcacaa ggggcaaacg tcctataatg agagacgacc ctgaagaaaa
    22441 atcgattatt cgctcaaata agattaatcc ctcttctatc agcaaaagta aagtttccgg
    22501 cagccagcga ttgaagagtg tgagaaataa cacaccagga agaaaaagaa acttgaatac
    22561 cggtgcaatc agtaatgtaa aaagactacc taaaataggc cactcataaa aatgccatac
    22621 aatcaacggc atcgtgatca tagtaaacag gacgtgagag gccgtttttt tcaaccaatc
    22681 tccatcaaaa gaaatcagga taaacaaaaa actcatgaaa aaactaagct gtccacccgc
    22741 atgaaaaaga aatcgaggat caaatagtaa aagaagccaa aagacaactg caaataagtc
    22801 catggtggaa taatgcttat tcagcaattt gactagcaat cgcaataaaa acagcaaact
    22861 agcacgcaaa acagaaatag aacttcctgc aatagaaaca taacagatag ttagtagact
    22921 caacggaata atactgacgt gtagtggcca tccgatacgt cgaaataaat aatacagcca
    22981 ccctaaataa aattggatgt gtagacccga tagactgaac agatgcagca aaccactctc
    23041 cctaaaaatt tcttctggtt ctctgaatcc tgcatcttta tagccccata ataaagaatt
    23101 aatatataca cttgtttttt gcggaaattc agattggaca aaggagataa aagatcctct
    23161 ttttctctga agccagtttc gccagtgatt ttgcatggtg atctgttcaa tcttttgaat
    23221 ttggaaccgt ccagaaattc tatggttcct gaagtaagct tcttggtcaa aattataaag
    23281 attccgcttt tttgctggag agatgaactc gccttcagca gtcaattcta ttccctttcc
    23341 tcggaagtta agccatgttt tcaattctgc ttctgtgttt acttgataag aaagctggag
    23401 cttttgttta ttctctgata accttccttc cattgtcaaa aaattaccat ttgcacgaat
    23461 cgtatctgta aatacagtaa tttgcgcaga tgttgttttt tcctctaaaa atgaatactc
    23521 ttgtagccta taagaaatgg ataccgttat gctacacaaa atacacaagt acgcgataaa
    23581 acgtccttct tttagcgaaa accataaaaa aattccggca agaagcaaac gataacccca
    23641 attgtcagtg taatacaaca aacagataat catcgacgta attgccggaa aaatcaaccg
    23701 atttcgaaca tgcctttcta aaacgctaga catgttttcc tgttaattca ttttgaagtt
    23761 ttgaaaaatg ctgtgagtct aaagcaactt ggtgtacaga tacacctaat tgttcgatca
    23821 acgcttgggc ataaggatca tttcgatagt catggagata attgatccct ttcacaccag
    23881 cttgcaacaa agcctttgta caggccaaac atggaaaatg tgtgacatag atttctgaac
    23941 cttccgtcgg gataccaagt tttgcacact gtaggagtgc attcatctct gcatggatcg
    24001 ttcgcaagca gtgtccatcc acgacgtaac acccttcatc tatgcagtgg acatctccac
    24061 tgactgctcc attatagccg ccagcaatga tccttttttc tcgaacgagt gtcgctccta
    24121 cttctaaacg tgtacacgta ctacgtaaag ataacagtac tgcctgagcc ataaagtact
    24181 ggtcccaagg aatgcgttca ttatgtgact tatcagtaac tttttcttta ttcgtatcgc
    24241 atgcttttga agatttttct aacatgtttt atattcctcc gttttttcaa gttgattaaa
    24301 aagtactcat tgcttaaaca aaatattctt taaatataac gaatcatcct gtctgacaaa
    24361 agatcctacc cttcactgcc gaaatgaagt ccgtcatcca ttagcccatt ataaaaattc
    24421 ttcgaatcca tttgttcaag aaattccgta taaaaatcga tacttcacaa tcatggcgtt
    24481 ttgctacttc tgtgagaaca gagatatatt ctttgatcaa cttgttattc cgatattttt
    24541 gttttgtctc atcgactgct ggtggcgtga taaagaaagc atgatcagaa ctatagtctt
    24601 ttttcagctg aactatcatc cactgcagat tttttggtac gtttctttag aaagttgttt
    24661 atgctctgct agatcattcg tcccaatgag aataaatact ttatctgctg tatagctaag
    24721 tacatctttg gcaaagcgtt ctcgtagagc ttgactgtta ttgccgctga cagccgtatt
    24781 cataatatga atcccagata ttttattttg gagatagaca ttcagcattg gtttttttaa
    24841 tgtttctttg cgaacaatca agctatttcc tatccaaaaa tactacctcc tcgtcctgcc
    24901 aagctcttag aattggatta ttcttagtat ccattagcgt ccatttattg ctcgtgccag
    24961 tcgattttga atcattctat ttaattgtaa cgaagttttt tagtttttca actgtctttt
    25021 caccgatacc agaaatctcc tgcaggtctt ccactttctg gaaggatccg ttttcttctc
    25081 taaatcgaat gatttcttgc gctttgactg atccgatccc aggaatctgc tgaagttctg
    25141 atggagtggc tgtattgata tttattttgg gaggctcgga atctgctgta ggctgttttc
    25201 cttccgagga tgccaactga tcaagatgtt ctggcagctc ttcacctaca gcagggatat
    25261 ataggacttg ctgatcttct atcttcgcag aaaagttgat tcgatcgcta tccgcttctt
    25321 ctaataatcc acctgcacgc tttagtacat catagacccg ttcttctgaa gaaaaggaat
    25381 agataccagg ctccttcaca cttcctttga tatcggtgta catgaccttt ggacgagcat
    25441 cctcttctac atcgtttcct tcagctgatg gatcttctcc actggaacta aacgaagaaa
    25501 gtatttcttt tgcagcagtc tcttctgttt ctctataaaa tagcaaattt gataataaaa
    25561 aaccacttgc gatacaaaag ataagaacta tcatagacat ccaaatgatc aatttgggtg
    25621 aagctagtat tttcttgctg attttttcta tcattgttct tttttcaaag aatgaccagt
    25681 aaaaatcatt cattactcct ttcatttaaa attgaagaaa cagtgagctc tggcaaaatc
    25741 gtcccttcaa catctaatta cgtcaatttt ttcttttgtt tttattttta tcgtgaagat
    25801 ttttgacgaa aaagaggggt tgtgacagaa ctcgtgtcac aacccctctt taatt
//
LOCUS       DAJSVP010000027        25700 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSVP010000027 DAJSVP010000000
VERSION     DAJSVP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.027"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            132..3659
                     /locus_tag="ON449_001230"
     CDS             132..3659
                     /locus_tag="ON449_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3933825.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="ON449_001231"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="ON449_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3933826.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="ON449_001232"
     CDS             7447..7788
                     /locus_tag="ON449_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3933827.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="ON449_001233"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="ON449_001233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3933828.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="ON449_001234"
     CDS             9145..11565
                     /locus_tag="ON449_001234"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3933829.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ON449_001235"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ON449_001235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3933830.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="ON449_001236"
     CDS             12999..13736
                     /locus_tag="ON449_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3933831.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="ON449_001237"
     CDS             13750..14565
                     /locus_tag="ON449_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3933832.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="ON449_001238"
     CDS             14578..15225
                     /locus_tag="ON449_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3933833.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="ON449_001239"
     CDS             15241..15897
                     /locus_tag="ON449_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3933834.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="ON449_001240"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="ON449_001240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3933835.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="ON449_001241"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="ON449_001241"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3933836.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="ON449_001242"
     CDS             18239..18757
                     /locus_tag="ON449_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3933837.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="ON449_001243"
     CDS             18864..19364
                     /locus_tag="ON449_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3933838.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="ON449_001244"
     CDS             complement(19644..20564)
                     /locus_tag="ON449_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3933839.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="ON449_001245"
     CDS             20729..21742
                     /locus_tag="ON449_001245"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3933840.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="ON449_001246"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="ON449_001246"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3933841.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="ON449_001247"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="ON449_001247"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3933842.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="ON449_001248"
     CDS             complement(23382..24143)
                     /locus_tag="ON449_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3933843.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="ON449_001249"
     CDS             24332..25426
                     /locus_tag="ON449_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3933844.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25512..>25700)
                     /locus_tag="ON449_001250"
     CDS             complement(25512..>25700)
                     /locus_tag="ON449_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3933845.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4455 c         5417 g         7319 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
    25501 gttatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt
    25561 tcgattggtg ttctgtaatt caatgatttt cttggaatat gattacgttg attgctgaca
    25621 ctggaaataa atgtctgatt cacttttcta aaatccattg atttcggcag tccattacga
    25681 cgcagaatcc cgttagaatt
//
LOCUS       DAJSVP010000028        25557 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSVP010000028 DAJSVP010000000
VERSION     DAJSVP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.028"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..847)
                     /locus_tag="ON449_001251"
     CDS             complement(<1..847)
                     /locus_tag="ON449_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3933846.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="ON449_001252"
     CDS             complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="ON449_001252"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3933847.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3348..3527)
                     /locus_tag="ON449_001253"
     CDS             complement(3348..3527)
                     /locus_tag="ON449_001253"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3933848.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3574..5397)
                     /gene="cadA"
                     /locus_tag="ON449_001254"
     CDS             complement(3574..5397)
                     /gene="cadA"
                     /locus_tag="ON449_001254"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3933849.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5675..7954
                     /locus_tag="ON449_001255"
     CDS             5675..7954
                     /locus_tag="ON449_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3933850.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8047..8694)
                     /locus_tag="ON449_001256"
     CDS             complement(8047..8694)
                     /locus_tag="ON449_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933851.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8873..9592)
                     /locus_tag="ON449_001257"
     CDS             complement(8873..9592)
                     /locus_tag="ON449_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3933852.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9723..10874)
                     /locus_tag="ON449_001258"
     CDS             complement(9723..10874)
                     /locus_tag="ON449_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3933853.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11091..11831)
                     /locus_tag="ON449_001259"
     CDS             complement(11091..11831)
                     /locus_tag="ON449_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3933854.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="ON449_001260"
     CDS             complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="ON449_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3933855.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="ON449_001261"
     CDS             complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="ON449_001261"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3933856.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12792..13442)
                     /locus_tag="ON449_001262"
     CDS             complement(12792..13442)
                     /locus_tag="ON449_001262"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3933857.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="ON449_001263"
     CDS             complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="ON449_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3933858.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="ON449_001264"
     CDS             complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="ON449_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3933859.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14885..15412)
                     /locus_tag="ON449_001265"
     CDS             complement(14885..15412)
                     /locus_tag="ON449_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3933860.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15592..16377)
                     /locus_tag="ON449_001266"
     CDS             complement(15592..16377)
                     /locus_tag="ON449_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3933861.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16370..17239)
                     /locus_tag="ON449_001267"
     CDS             complement(16370..17239)
                     /locus_tag="ON449_001267"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3933862.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17240..18619)
                     /locus_tag="ON449_001268"
     CDS             complement(17240..18619)
                     /locus_tag="ON449_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3933863.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="ON449_001269"
     CDS             complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="ON449_001269"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3933864.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19071..19547)
                     /gene="accB"
                     /locus_tag="ON449_001270"
     CDS             complement(19071..19547)
                     /gene="accB"
                     /locus_tag="ON449_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3933865.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="ON449_001271"
     CDS             complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="ON449_001271"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3933866.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="ON449_001272"
     CDS             complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="ON449_001272"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3933867.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="ON449_001273"
     CDS             complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="ON449_001273"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3933868.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22577..22801)
                     /locus_tag="ON449_001274"
     CDS             complement(22577..22801)
                     /locus_tag="ON449_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3933869.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22854..23816)
                     /locus_tag="ON449_001275"
     CDS             complement(22854..23816)
                     /locus_tag="ON449_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3933870.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23826..24266)
                     /locus_tag="ON449_001276"
     CDS             complement(23826..24266)
                     /locus_tag="ON449_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3933871.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24572..25537
                     /locus_tag="ON449_001277"
     CDS             24572..25537
                     /locus_tag="ON449_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3933872.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         7087 a         5635 c         4436 g         8399 t
ORIGIN      
        1 atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa
       61 gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca
      121 aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga
      181 gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga
      241 tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg
      301 aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga
      361 tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta
      421 ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact
      481 ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt
      541 gtgcattgtt accaagtttg tttttgagag agtaaaagga ctcatttgct tctatatcac
      601 taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca
      661 gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag
      721 cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt
      781 tggccaactt tttttctgct gaagaataat cttccatcat actttcaata cgactaatta
      841 cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc
      901 ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt
      961 atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct
     1021 attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct
     1081 ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca
     1141 gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct
     1201 ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt
     1261 ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt
     1321 tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt
     1381 gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc
     1441 attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct
     1501 ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat
     1561 gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct
     1621 gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt
     1681 gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg
     1741 cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc
     1801 aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca
     1861 tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag
     1921 cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg
     1981 tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca
     2041 tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt
     2101 ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc tagactaact
     2161 tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag
     2221 aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt
     2281 ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc
     2341 ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc
     2401 caataagcac ctgccactga taataattta aatacttgta tacggccagt ggatggaaca
     2461 tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct
     2521 tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct
     2581 tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt
     2641 tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg
     2701 tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga
     2761 cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca
     2821 tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg
     2881 agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca
     2941 gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta
     3001 atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa
     3061 aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc
     3121 acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct
     3181 tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag
     3241 cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt
     3301 gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat
     3361 tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct
     3421 tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat
     3481 tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct
     3541 ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga
     3601 atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc
     3661 cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa
     3721 catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt
     3781 atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc
     3841 atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc
     3901 agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc
     3961 tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga
     4021 atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat
     4081 catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg
     4141 aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc
     4201 tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc
     4261 atgattattg actactgctt ggattccttg accggtcact tctttcagtt gactaacagg
     4321 atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc
     4381 ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg
     4441 aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt
     4501 ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact
     4561 tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga
     4621 tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc
     4681 accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa
     4741 tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc
     4801 tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat
     4861 aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc
     4921 gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac
     4981 tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt
     5041 gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg
     5101 tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact
     5161 agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac
     5221 gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat
     5281 ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact
     5341 taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt
     5401 tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc
     5461 atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac
     5521 tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat
     5581 tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta
     5641 attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac
     5701 gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca
     5761 acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg
     5821 agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga
     5881 attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt
     5941 catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac
     6001 attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg
     6061 gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac
     6121 tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg
     6181 acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat
     6241 attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca
     6301 aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag
     6361 tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg
     6421 gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc
     6481 gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt
     6541 ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt
     6601 gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat
     6661 tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa
     6721 tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca
     6781 attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg
     6841 tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca
     6901 agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg
     6961 taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat
     7021 gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact
     7081 ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt
     7141 tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact
     7201 gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct ttattgatga
     7261 aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa
     7321 tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc
     7381 attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg
     7441 ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga
     7501 aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa
     7561 attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac
     7621 agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga
     7681 gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat
     7741 tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt
     7801 agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg
     7861 tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat
     7921 ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat
     7981 gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa
     8041 ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga
     8101 aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg
     8161 acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc
     8221 aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa
     8281 tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc
     8341 gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc
     8401 catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg
     8461 aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata
     8521 acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct
     8581 tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt
     8641 gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt
     8701 ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac
     8761 cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga
     8821 gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt
     8881 ctaatgccac attatggaac acatgctgta catcttctag atcttccagt gcatcaatca
     8941 ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt
     9001 ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat
     9061 ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa
     9121 catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag
     9181 caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc
     9241 catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg
     9301 tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg
     9361 tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat
     9421 tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat
     9481 cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat
     9541 tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac
     9601 ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga
     9661 atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt
     9721 atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga
     9781 tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg
     9841 gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca
     9901 agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc
     9961 aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt
    10021 gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt
    10081 ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa
    10141 cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct
    10201 gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct
    10261 gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt
    10321 cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat
    10381 cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa
    10441 acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct
    10501 tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc
    10561 tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca
    10621 cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa
    10681 tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg
    10741 cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt
    10801 cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc
    10861 ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag
    10921 taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca
    10981 atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa
    11041 tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg
    11101 tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa
    11161 aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga
    11221 taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct
    11281 tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc
    11341 cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct
    11401 acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag
    11461 acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa
    11521 aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca
    11581 aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct
    11641 gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca
    11701 gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa
    11761 gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt
    11821 tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat
    11881 agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct
    11941 ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat
    12001 ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta
    12061 tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg
    12121 ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta
    12181 ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat
    12241 atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat
    12301 gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt
    12361 gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa
    12421 ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat
    12481 cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat
    12541 tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct
    12601 catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag
    12661 aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga
    12721 agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag
    12781 tatattcatt actatagtcc atattcatac aacccttcat tttgaatata atcaattact
    12841 ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg
    12901 gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg
    12961 tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac
    13021 cattttggca aatattctac catatcccca ccgataataa agtaataatc tgtatccgga
    13081 ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat
    13141 tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt
    13201 cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc
    13261 aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca
    13321 aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat
    13381 tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc
    13441 atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg
    13501 gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct
    13561 ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact
    13621 ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc
    13681 ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc
    13741 ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc
    13801 aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc
    13861 cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca
    13921 agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt
    13981 ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac
    14041 acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc
    14101 aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt
    14161 gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt
    14221 cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc
    14281 gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt
    14341 tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca
    14401 tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta
    14461 gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt
    14521 gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt
    14581 actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa
    14641 atatccacca cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa
    14701 aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa
    14761 cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat
    14821 cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct
    14881 tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca
    14941 taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg
    15001 gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct
    15061 gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga
    15121 tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg
    15181 ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt
    15241 tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca
    15301 acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg
    15361 taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc
    15421 ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat
    15481 atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa
    15541 aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg
    15601 gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt
    15661 caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc
    15721 gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc
    15781 agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga
    15841 cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc
    15901 cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat
    15961 cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg
    16021 ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct
    16081 attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg
    16141 agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac
    16201 tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa
    16261 taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct
    16321 tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat
    16381 ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca
    16441 tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat
    16501 cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca
    16561 agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc
    16621 cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga
    16681 cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc
    16741 cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc
    16801 tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca
    16861 ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa
    16921 gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat
    16981 ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta
    17041 gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac
    17101 cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag
    17161 gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga atatatttct
    17221 ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta
    17281 aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa
    17341 tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg
    17401 ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa
    17461 tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca
    17521 gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat
    17581 ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt
    17641 cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat
    17701 cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca
    17761 tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat
    17821 tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga
    17881 tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc
    17941 gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc
    18001 gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt
    18061 cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc
    18121 ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta
    18181 tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg actgggacat
    18241 ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac
    18301 ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt
    18361 cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca
    18421 gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt
    18481 catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt
    18541 ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta
    18601 tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct
    18661 acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc
    18721 aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct
    18781 ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca
    18841 actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct
    18901 ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt
    18961 tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg
    19021 atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc
    19081 tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac aaggatttct
    19141 gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg
    19201 cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc
    19261 gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact
    19321 gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg
    19381 actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt
    19441 ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag
    19501 gattgatcaa attgactgac taattctctg atttcattga tatccaaaat tattcctccc
    19561 agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat
    19621 aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt
    19681 gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca
    19741 aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga
    19801 cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt
    19861 cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac
    19921 cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag
    19981 ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg
    20041 caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca
    20101 tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt
    20161 ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg
    20221 ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa
    20281 ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta
    20341 cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag
    20401 aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac
    20461 caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta
    20521 aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca
    20581 ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg
    20641 ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc
    20701 cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat
    20761 agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca
    20821 atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg
    20881 ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt
    20941 gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa
    21001 tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta
    21061 ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc aacccagata
    21121 cactagagag attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct
    21181 gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat
    21241 ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg
    21301 atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt
    21361 caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg
    21421 gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca
    21481 tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct tttacatcca
    21541 taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg
    21601 cagaactggt attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc
    21661 gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg
    21721 aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc
    21781 cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc
    21841 cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt
    21901 tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc
    21961 tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat
    22021 gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac
    22081 tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc
    22141 tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc
    22201 tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata
    22261 atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc
    22321 attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa
    22381 tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc
    22441 ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt
    22501 ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc
    22561 aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg
    22621 ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga
    22681 tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga
    22741 tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca
    22801 taataatata ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac
    22861 tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc
    22921 tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt
    22981 gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt
    23041 acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca
    23101 actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca
    23161 aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac
    23221 ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct
    23281 aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca
    23341 tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca
    23401 ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac
    23461 gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc
    23521 aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct
    23581 actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc
    23641 actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc
    23701 gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc attattagta
    23761 ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa
    23821 tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca
    23881 tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc
    23941 tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg
    24001 tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg
    24061 gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg
    24121 tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat
    24181 tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac aagataatca
    24241 tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa
    24301 tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt
    24361 ttttactata tgagcttttt ttaagatttt actcatttaa aacaactgat aaatcagcat
    24421 ttataatttc ttttattaga aaaatcatct atctcacata ttttgattat caaagtattt
    24481 tctgtgaaca gaactataga attccctaaa acttgtccat tatttcaaaa tttgttatag
    24541 tctttttatt caaagcgtaa aaggagatcc tatgttaaaa gaaaaaatga aaactctccc
    24601 catgctgccg ggtgtctatc ttatgaaaga ccgagatggt aaggtcatct atgttggtaa
    24661 agcaaaacgg ctcaaaaata gagtaagcag ctattttcat cagaataaac agcattcaaa
    24721 aaaagtatta cgtatgattc atcatataac agattttgat ttcgtcgtag tagacacgga
    24781 attagatgct ttattgctcg agtgtcaatt gatccaacat taccgtcctt tttataaccg
    24841 acaaatgaat tatttcagta attataatta tgtacatatt accaataagg gatttgtatt
    24901 aacagatact cctacggctc gaacatacgg gccttttcgt ctctataaaa aaatgccgtc
    24961 tattttgcgt ataatggaag aaacttatca aatgccttgg ttgtctgaaa ttagtttgtt
    25021 agctttgcga gtccagcttc ctgacctaca ggaaatgact tttgaacaga aaaaaaagga
    25081 actccaaggg ctgtttcaag gccgaaataa aaagctactg acttacttga aaaaaagaca
    25141 acaacatttc atctatcagt tgaatttcga aaaagccggt atgcttcaaa aagatatcga
    25201 attagttact tattttatta gacggatcca ggaacaaaag cagtttttaa gaacaccatc
    25261 gctcaccttc agcatgccat tagcagcaga tgagagtcag aaaaagcact acctcgtttg
    25321 ttatggtcaa cttgcagaaa caatgattac ctctggtgac gtttctcctg acttctatta
    25381 tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg tcaaaagaag aaatcgatcc
    25441 tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa gaacagtttg aaattgaact
    25501 gttaaacaaa aaagaagctg aaaagcaact aaattagttg cttctcggct tctttta
//
LOCUS       DAJSVP010000029        24534 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSVP010000029 DAJSVP010000000
VERSION     DAJSVP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.029"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..1183
                     /locus_tag="ON449_001278"
     CDS             185..1183
                     /locus_tag="ON449_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3933873.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ON449_001279"
     CDS             1295..1804
                     /locus_tag="ON449_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3933874.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ON449_001280"
     CDS             complement(1834..2493)
                     /locus_tag="ON449_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3933875.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ON449_001281"
     CDS             complement(2495..3259)
                     /locus_tag="ON449_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3933876.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ON449_001282"
     CDS             complement(3267..3944)
                     /locus_tag="ON449_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3933877.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ON449_001283"
     CDS             complement(3981..5366)
                     /locus_tag="ON449_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3933878.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ON449_001284"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON449_001284"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON449_001285"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON449_001285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3933879.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ON449_001286"
     CDS             6305..7162
                     /locus_tag="ON449_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933880.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ON449_001287"
     CDS             complement(7243..7578)
                     /locus_tag="ON449_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933881.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ON449_001288"
     CDS             complement(7645..9015)
                     /locus_tag="ON449_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3933882.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ON449_001289"
     CDS             complement(9093..9629)
                     /locus_tag="ON449_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3933883.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ON449_001290"
     CDS             9938..11419
                     /locus_tag="ON449_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3933884.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="ON449_001291"
     CDS             complement(11522..12229)
                     /locus_tag="ON449_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3933885.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="ON449_001292"
     CDS             complement(12308..12646)
                     /locus_tag="ON449_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3933886.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="ON449_001293"
     CDS             complement(12757..13719)
                     /locus_tag="ON449_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3933887.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="ON449_001294"
     CDS             complement(13734..15065)
                     /locus_tag="ON449_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3933888.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="ON449_001295"
     CDS             complement(15073..15753)
                     /locus_tag="ON449_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3933889.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="ON449_001296"
     CDS             complement(15972..16451)
                     /locus_tag="ON449_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3933890.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="ON449_001297"
     CDS             complement(16578..18257)
                     /locus_tag="ON449_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3933891.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="ON449_001298"
     CDS             18805..20307
                     /locus_tag="ON449_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933892.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="ON449_001299"
     CDS             20320..20982
                     /locus_tag="ON449_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933893.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="ON449_001300"
     CDS             complement(21083..21286)
                     /locus_tag="ON449_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3933894.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="ON449_001301"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="ON449_001301"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3933895.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="ON449_001302"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="ON449_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3933896.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         7260 a         5178 c         4168 g         7928 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctatcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaa
//
LOCUS       DAJSVP010000030        24247 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSVP010000030 DAJSVP010000000
VERSION     DAJSVP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.030"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(56..496)
                     /locus_tag="ON449_001303"
     CDS             complement(56..496)
                     /locus_tag="ON449_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3933897.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            654..1418
                     /gene="map"
                     /locus_tag="ON449_001304"
     CDS             654..1418
                     /gene="map"
                     /locus_tag="ON449_001304"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3933898.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            1450..2373
                     /locus_tag="ON449_001305"
     CDS             1450..2373
                     /locus_tag="ON449_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3933899.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            2449..3588
                     /locus_tag="ON449_001306"
     CDS             2449..3588
                     /locus_tag="ON449_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3933900.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            3581..4381
                     /locus_tag="ON449_001307"
     CDS             3581..4381
                     /locus_tag="ON449_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3933901.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            4381..5208
                     /locus_tag="ON449_001308"
     CDS             4381..5208
                     /locus_tag="ON449_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3933902.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5186..5920
                     /locus_tag="ON449_001309"
     CDS             5186..5920
                     /locus_tag="ON449_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3933903.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6020..6886
                     /gene="rfbA"
                     /locus_tag="ON449_001310"
     CDS             6020..6886
                     /gene="rfbA"
                     /locus_tag="ON449_001310"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3933904.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            6900..7472
                     /gene="rfbC"
                     /locus_tag="ON449_001311"
     CDS             6900..7472
                     /gene="rfbC"
                     /locus_tag="ON449_001311"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3933905.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            7494..8522
                     /gene="rfbB"
                     /locus_tag="ON449_001312"
     CDS             7494..8522
                     /gene="rfbB"
                     /locus_tag="ON449_001312"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3933906.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            8620..9471
                     /gene="rfbD"
                     /locus_tag="ON449_001313"
     CDS             8620..9471
                     /gene="rfbD"
                     /locus_tag="ON449_001313"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3933907.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            9506..11539
                     /locus_tag="ON449_001314"
     CDS             9506..11539
                     /locus_tag="ON449_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933908.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            11583..12863
                     /locus_tag="ON449_001315"
     CDS             11583..12863
                     /locus_tag="ON449_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3933909.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13073..13879
                     /locus_tag="ON449_001316"
     CDS             13073..13879
                     /locus_tag="ON449_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933910.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            13891..15111
                     /locus_tag="ON449_001317"
     CDS             13891..15111
                     /locus_tag="ON449_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933911.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15101..16687
                     /locus_tag="ON449_001318"
     CDS             15101..16687
                     /locus_tag="ON449_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3933912.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            16726..18864
                     /locus_tag="ON449_001319"
     CDS             16726..18864
                     /locus_tag="ON449_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3933913.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(19232..20215)
                     /locus_tag="ON449_001320"
     CDS             complement(19232..20215)
                     /locus_tag="ON449_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3933914.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            20593..21984
                     /locus_tag="ON449_001321"
     CDS             20593..21984
                     /locus_tag="ON449_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3933915.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22000..23040
                     /locus_tag="ON449_001322"
     CDS             22000..23040
                     /locus_tag="ON449_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3933916.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23059..24144
                     /locus_tag="ON449_001323"
     CDS             23059..24144
                     /locus_tag="ON449_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3933917.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         7792 a         3993 c         4967 g         7495 t
ORIGIN      
        1 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
       61 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
      121 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
      181 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
      241 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
      301 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
      361 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
      421 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
      481 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
      541 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
      601 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
      661 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
      721 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
      781 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
      841 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
      901 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
      961 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     1021 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     1081 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     1141 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     1201 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     1261 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     1321 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     1381 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     1441 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     1501 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     1561 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     1621 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     1681 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     1741 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     1801 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     1861 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     1921 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     1981 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     2041 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     2101 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     2161 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     2221 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     2281 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     2341 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     2401 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     2461 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     2521 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     2581 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     2641 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     2701 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     2761 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     2821 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     2881 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     2941 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     3001 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     3061 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     3121 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     3181 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     3241 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     3301 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     3361 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     3421 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     3481 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     3541 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     3601 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     3661 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     3721 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     3781 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     3841 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     3901 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     3961 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     4021 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     4081 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     4141 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     4201 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     4261 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     4321 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     4381 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     4441 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     4501 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     4561 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     4621 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     4681 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     4741 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     4801 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     4861 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     4921 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     4981 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     5041 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     5101 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     5161 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     5221 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     5281 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     5341 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     5401 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     5461 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     5521 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     5581 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     5641 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     5701 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     5761 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     5821 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     5881 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     5941 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     6001 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     6061 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     6121 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     6181 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     6241 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     6301 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     6361 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     6421 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     6481 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     6541 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     6601 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     6661 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     6721 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
     6781 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
     6841 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
     6901 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
     6961 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
     7021 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
     7081 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
     7141 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
     7201 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
     7261 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
     7321 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
     7381 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
     7441 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
     7501 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
     7561 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
     7621 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
     7681 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
     7741 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
     7801 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
     7861 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
     7921 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
     7981 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
     8041 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
     8101 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
     8161 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
     8221 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
     8281 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
     8341 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
     8401 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
     8461 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
     8521 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
     8581 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
     8641 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
     8701 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
     8761 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
     8821 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
     8881 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
     8941 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
     9001 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
     9061 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
     9121 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
     9181 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
     9241 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
     9301 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
     9361 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
     9421 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
     9481 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
     9541 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
     9601 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
     9661 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
     9721 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
     9781 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
     9841 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
     9901 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
     9961 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
    10021 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
    10081 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
    10141 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
    10201 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
    10261 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
    10321 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
    10381 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
    10441 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
    10501 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
    10561 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
    10621 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
    10681 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
    10741 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
    10801 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
    10861 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
    10921 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
    10981 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
    11041 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
    11101 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
    11161 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
    11221 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
    11281 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
    11341 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
    11401 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
    11461 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
    11521 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
    11581 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
    11641 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
    11701 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
    11761 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
    11821 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
    11881 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
    11941 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
    12001 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    12061 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    12121 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
    12181 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    12241 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    12301 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    12361 aaaaagtcaa tttgtttacc gatcgagaag atatttggca taaattctat cagacgctag
    12421 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
    12481 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    12541 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    12601 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    12661 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
    12721 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    12781 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    12841 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    12901 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    12961 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    13021 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    13081 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    13141 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    13201 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    13261 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    13321 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    13381 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    13441 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    13501 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    13561 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    13621 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    13681 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    13741 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    13801 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    13861 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    13921 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    13981 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    14041 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    14101 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    14161 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    14221 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    14281 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    14341 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    14401 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    14461 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    14521 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    14581 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    14641 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    14701 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    14761 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    14821 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    14881 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    14941 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    15001 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    15061 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    15121 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    15181 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    15241 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    15301 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    15361 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    15421 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    15481 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    15541 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    15601 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    15661 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    15721 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    15781 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    15841 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    15901 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    15961 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    16021 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    16081 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    16141 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    16201 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    16261 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    16321 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    16381 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    16441 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    16501 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    16561 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    16621 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    16681 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    16741 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    16801 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    16861 gtgattgctg aatcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    16921 gttaagaccc aaagcggttt caaggtacgt ttatcaggga aaatgagagg aaaagccatc
    16981 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    17041 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    17101 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    17161 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    17221 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    17281 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    17341 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    17401 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    17461 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    17521 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    17581 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    17641 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    17701 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    17761 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    17821 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    17881 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    17941 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    18001 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    18061 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    18121 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    18181 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    18241 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    18301 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    18361 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    18421 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    18481 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    18541 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    18601 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    18661 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    18721 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    18781 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga cggaatcggc
    18841 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    18901 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    18961 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    19021 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    19081 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    19141 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    19201 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    19261 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    19321 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    19381 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    19441 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    19501 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    19561 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    19621 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    19681 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    19741 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    19801 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    19861 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    19921 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    19981 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    20041 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    20101 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    20161 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    20221 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    20281 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    20341 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    20401 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    20461 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    20521 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
    20581 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    20641 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    20701 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    20761 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    20821 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    20881 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    20941 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    21001 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    21061 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    21121 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    21181 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    21241 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    21301 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    21361 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    21421 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    21481 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    21541 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    21601 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    21661 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    21721 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    21781 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    21841 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    21901 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    21961 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    22021 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
    22081 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    22141 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    22201 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    22261 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    22321 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    22381 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    22441 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    22501 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    22561 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
    22621 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
    22681 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
    22741 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
    22801 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
    22861 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
    22921 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
    22981 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
    23041 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
    23101 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
    23161 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
    23221 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
    23281 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
    23341 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
    23401 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
    23461 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
    23521 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
    23581 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
    23641 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
    23701 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
    23761 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
    23821 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
    23881 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
    23941 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
    24001 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
    24061 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
    24121 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
    24181 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
    24241 gattgaa
//
LOCUS       DAJSVP010000031        23703 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSVP010000031 DAJSVP010000000
VERSION     DAJSVP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.031"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ON449_001324"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ON449_001324"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3933918.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ON449_001325"
     CDS             complement(1173..1868)
                     /locus_tag="ON449_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3933919.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ON449_001326"
     CDS             complement(1881..2597)
                     /locus_tag="ON449_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3933920.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="ON449_001327"
     CDS             2927..3535
                     /locus_tag="ON449_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3933921.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3860..4183
                     /locus_tag="ON449_001328"
     CDS             3860..4183
                     /locus_tag="ON449_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3933922.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4365..5735
                     /locus_tag="ON449_001329"
     CDS             4365..5735
                     /locus_tag="ON449_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3933923.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5865..7166)
                     /locus_tag="ON449_001330"
     CDS             complement(5865..7166)
                     /locus_tag="ON449_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3933924.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7520..9478)
                     /locus_tag="ON449_001331"
     CDS             complement(7520..9478)
                     /locus_tag="ON449_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3933925.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9655..9921)
                     /locus_tag="ON449_001332"
     CDS             complement(9655..9921)
                     /locus_tag="ON449_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3933926.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="ON449_001333"
     CDS             complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="ON449_001333"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3933927.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="ON449_001334"
     CDS             complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="ON449_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3933928.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13265..13828
                     /locus_tag="ON449_001335"
     CDS             13265..13828
                     /locus_tag="ON449_001335"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3933929.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14081..14602)
                     /locus_tag="ON449_001336"
     CDS             complement(14081..14602)
                     /locus_tag="ON449_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3933930.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14605..15447)
                     /locus_tag="ON449_001337"
     CDS             complement(14605..15447)
                     /locus_tag="ON449_001337"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3933931.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15440..16165)
                     /locus_tag="ON449_001338"
     CDS             complement(15440..16165)
                     /locus_tag="ON449_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3933932.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16341..16637
                     /locus_tag="ON449_001339"
     CDS             16341..16637
                     /locus_tag="ON449_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933933.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17205..18797
                     /locus_tag="ON449_001340"
     CDS             17205..18797
                     /locus_tag="ON449_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3933934.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18810..19550
                     /locus_tag="ON449_001341"
     CDS             18810..19550
                     /locus_tag="ON449_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3933935.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19718..21067
                     /locus_tag="ON449_001342"
     CDS             19718..21067
                     /locus_tag="ON449_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933936.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21195..21785)
                     /locus_tag="ON449_001343"
     CDS             complement(21195..21785)
                     /locus_tag="ON449_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3933937.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21932..23284
                     /gene="brnQ"
                     /locus_tag="ON449_001344"
     CDS             21932..23284
                     /gene="brnQ"
                     /locus_tag="ON449_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3933938.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6797 a         4772 c         4511 g         7623 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcaaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc ttttttattt
     3601 ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca aacgaaaaat
     3661 cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa atttatactt
     3721 tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga atgaaccctc
     3781 gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa cattcgttga
     3841 ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc gttacgcttc
     3901 agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt ttattattga
     3961 cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt tgatcgaaaa
     4021 tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa tggatattga
     4081 tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg atgacggtac
     4141 gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata gataaacgtg
     4201 gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa ttcttggctt
     4261 ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca ggataaacgc
     4321 tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc gatttaacaa
     4381 aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc ggaaatgttt
     4441 ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta ggtaaaaatg
     4501 cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc tttgcccaag
     4561 gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa gattatcggg
     4621 gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact gtgattttaa
     4681 ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact cctcctgaaa
     4741 tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt gcatcagtca
     4801 gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca ccactattct
     4861 ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt atctattttg
     4921 gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct tccagcgtac
     4981 tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa aaagatttca
     5041 aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg gcgtttcaat
     5101 cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt ctagggacag
     5161 atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat cagccgatga
     5221 tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg aaagaatatg
     5281 gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt agtctcttag
     5341 caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt agcccatcag
     5401 aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct ctttactgga
     5461 tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa agcaagatcc
     5521 ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc gttcttacag
     5581 gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc ggttctgttg
     5641 ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg ggtgaagagg
     5701 aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa atcgttgcgg
     5761 ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca ggttttttag
     5821 ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc tgtttttctt
     5881 tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa taccgtgaaa
     5941 cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg gctagaagca
     6001 aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac atgaaaataa
     6061 cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc tgactgttcc
     6121 gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat ttcttctaaa
     6181 atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa gatagcaata
     6241 tgcttaccct tttttcgcat gatcgtcaat aattttttta tagggatcgt ttcagttgtt
     6301 acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata agattgcttg
     6361 atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa gataaccgga
     6421 tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt ttctgaagct
     6481 tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt atcatcaaat
     6541 tcaaacacgt tttccaaata ataatattct tctttggtca actctccttt ttcataagaa
     6601 ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc agatacgaga
     6661 cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca aataaaagga
     6721 aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac cttctccgta
     6781 ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat atacgtaact
     6841 aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa aggtagttta
     6901 ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc gatgatcaaa
     6961 ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg gtgtgtaaca
     7021 tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa tcttgatcgt
     7081 ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc aatcatcaaa
     7141 aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct attgtaccct
     7201 ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa aaggagatgc
     7261 ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt gttgctaaac
     7321 agctttttca tgacctctga taaaaacggt attcaaaagc ttgcatctac ggcattaagc
     7381 ccaaacagat gaaaaacatg agaagctgtt tttatctgct cgttcttact gcttgttgca
     7441 gtacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7501 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7561 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7621 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7681 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7741 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7801 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7861 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7921 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7981 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     8041 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     8101 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     8161 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     8221 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     8281 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     8341 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     8401 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8461 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8521 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8581 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8641 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8701 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8761 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8821 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8881 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8941 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     9001 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     9061 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     9121 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     9181 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     9241 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     9301 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     9361 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9421 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagccattg
     9481 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9541 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9601 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9661 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9721 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9781 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9841 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9901 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9961 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
    10021 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaatcaga
    10081 ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    10141 atattttgcc gttttttgag ctaatatcga gggacagctg attgaacatc gtttattgga
    10201 gaggttgtaa aaaagctgtt ctgcatcaag taataagaac agccaaataa aaacagctcc
    10261 taatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10321 tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc ccttttttcg aataattatt
    10381 gtccccctgt caccttcacg atcacaagta cattattcct atgaattaaa ttatttttct
    10441 tcatttttcg ttgaaccatc ttcgttcaac attgcttttc ttgacagatt tacgcgtcct
    10501 tgtttatcga tttcagttac tttcacaagg atctcatcac ctagtttaac aacatcttcg
    10561 actttattga cacgatcgtt tgctaattgg gaaatatgga caagtccatc ttttcctttg
    10621 atcaagttga cgaatgcacc gaatttttcg atacggacaa ctttccctag ataaacttca
    10681 ccaactttga cttctttggt caaatcttcg atgatcttga tagctttttt gatcatttct
    10741 gcatcagctg aagcaatact tacttttcca tcttgatcga tatcgatttt cacaccagtt
    10801 tcatcgatga ttccgttgat cgtatcgccg cctttaccga taacatcttt gattttagca
    10861 ggttcgatct ggatcatttc gatttttggt gcatattggc ttaattcttc acgtggagca
    10921 gcaatcgttg aagtcaattc ttctaagatt tccatacgtg cttgttttgc ttgagttaat
    10981 gcttcagtta ggatttgttc tgtgattcct tgaatcttga tatccatctg taaagcagtg
    11041 ataccatctt ttgtaccggc aactttaaag tccatatcgc caagatgatc ttccagccct
    11101 tggatatcag ttaggatcgt atagttttca ccatcagaaa caagtcccat cgcaataccg
    11161 gcgactggcg ctttgattgg tacacctgca tccatcaatg ctaatgtgcc agcacagata
    11221 cttgcttggg aagaagaacc atttgattcc aatacttctg ctactaaacg gatcgtataa
    11281 ggaaattctt cttctgaagg gattacttgt gctaacgcac gttcacctaa tgcgccatgt
    11341 ccgatttccc gacgccctgg agagcctgca cgtcctgtag aaccaacaga aaattgaggg
    11401 aaattgtagt ggtggatgaa tcgtttgctg acttccaccc ctaaaccatc aatgatttgg
    11461 tgctctccta gaggtgcgag tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac
    11521 aaacctgatc catgtacacg tggcaataag ctgacctctg atgacaatgg acggatttca
    11581 tccaatttac gcccgtcagg acggatctta tcgatggtga tcaattcacg tacgacatct
    11641 ttttcaagat cttctgtgat ttgtttaact tctttcaaca agtgatcttc atcttcatga
    11701 cctgcaaatt tctcagcata agcttcgcgg atatcgatct tcacctgctc gatattatct
    11761 tcgcgagcaa gtttttcttc agtcattact gctgctttca ttgtagcgta agaagcatca
    11821 aagatttctt ttttcaatac ttcatcaact tgcagtaaag tcacttccat tttttctttc
    11881 cctactgctt gaacgatttc ttcttggaaa gcaactaact ctttgatagc gtcaaaacca
    11941 aacagcaatg caccaagcat gtcttcttct gaaacttctt tcgcgccgct ttcaaccata
    12001 ttgatggctt gtttcgttcc ggcaacagtc aattcgatat ctgtttgttc tgcttgttct
    12061 actgttggat tcaagacgta ctcgccattt acacgaccaa cttctacgcc tgcgattggg
    12121 ccgtcaaatg ggatatctga gattgatagt gctaaagaag aacctagcat tgctgccata
    12181 gctggcgagc agtctgtttc tacactcatc acgatgttcg ttacttggac ttcattacgg
    12241 aagccttctg caaacatcgg gcgaattgga cggtcgatca aacgggcagt caatgttgct
    12301 tccgtacttg gacggccttc tcgtttgatg aaacctccag ggattttccc tgctgcatac
    12361 attttttctt cataattgat cgttaatggg aaaaagtctg tatcttttgc ttctttagag
    12421 gccacagctg cacttaagac tactgtatct ccataacgga ctaaaacggc tccgtttgct
    12481 tgttttgcta gctgaccgac ttcgatttgt aagggacggc cgccccatgt cgttttgaat
    12541 acttgttttt ctgacataac ttctcctttt ccttctcgtg aaagcaagac gctactaaac
    12601 aaatgaaaac tgccgaaagc cgtcttttga gcagctttca tttggttagt aggagtccat
    12661 ctagcttgat cacggaattt tacttaattg atatacgagt aaaaaaagtg agattcatgt
    12721 gaatcccact ctttttgact aaaattaacg acgcaagcct aaacgtttga tcaattcgcg
    12781 gtaacgttga acgtcagttt tacgtaagta agctaacaag ttacggcggt gaccaacttt
    12841 tttcattagg ccgcggtatg aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa
    12901 gtggttgatt tcttcagtta atacagcgat ttgtacttct ggagaaccag tatccccttc
    12961 atggcgagcg tattcgttga tgatttcatt cttgcgttct tttgaaattg ccatttcttt
    13021 cacctctttc gattattccc ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt
    13081 ggcaggtcat gtgcctagag cacacagtta tactttacct gaaaccgggt tgaaaatcaa
    13141 gtagttaatc attaagaaaa ggtagttttc aaacaatctt actactactt ttccattttt
    13201 gtggtaaact ataatgcgaa aatagacttg aaacacagtc ttttaagaaa ggggtacata
    13261 ttccatgatt acaatggatg atatcattcg tgaaggaaat ccgaccttac gcgaagttgc
    13321 aaaagaagtt tctcttccat taagcgaaga agatatctct ttaggaaaag agatgcttga
    13381 gttcttaaaa aacagccaag atccaatcaa agctgaagaa ctacacttac gaggcggtgt
    13441 tggcttagct gctcctcaat tagatatttc taaaagaatc attgctgttc acgtaccaag
    13501 tcctgaccca gaggcagatg gaccttctct aagtactgtt atgtataatc ctaaaatttt
    13561 gagccattct gttcaagatg cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg
    13621 agaagtacca ggatatgttg ttcgccatgc caaaatcact gtgtcttatt atgatatgaa
    13681 tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat
    13741 cgaccatatc aacggtgtga tgttctatga ccacatcaac gatcaaaatc catttgcact
    13801 aaaagaaggc gtattggtga tcgagtagag aattggcagg ttccttcgat actatgctac
    13861 aagaaagctt tataaaaaag gggagtgctc aaaagctgca cttccctttt ttatgtttct
    13921 ccactaatcg aaatttagat agacatctag aaatcgcaat tccaaaaaat tctgtgtatt
    13981 ggatacgcat cttttcttat tttttcttat ttcttccata tgggtgttta tctcagagat
    14041 gatagaatag tttctaggat aaaaagtagc agctacgatg ttatccttcg tagaggattc
    14101 ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg cttttctggg tagcggatcg
    14161 tatgcagatg gatgccgcct gttaattcgg ataacaaagt agcattactt ttttgataac
    14221 gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg caaagttcct gctaactcac
    14281 cgtaaagggg atgttccaca attacatcaa tgatttcacc accattttca acgattagtc
    14341 tcagttcttc ttctgtttga tcttttccat gctggacagc gattttactg atgtgcccag
    14401 tattggtttc ttctgtttcg aggatataac ctcttgctgt tgccacgata cttttacctg
    14461 cagctcgtag taaagcaata tcgcctacaa tgatctgacg gctgactcca aaagttttcg
    14521 caaacttgct agcactcact ggtttttctg tatttgccaa ggtttgcatg atttgttttc
    14581 gccgtgtctc tccttcgatc acacttattc ctccttcatt ttgttttggt attcgcgcga
    14641 gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc ttatatccct tatttttcag
    14701 cagactaaca actttatcca atacttgctg ctctcttttt gcatcataaa ctgcttggtt
    14761 ttcttctttt ttcacttgtc ctattttagt taccgtatcc attctttttt ctaataaagc
    14821 aaccaactct cgatcgatca tatcgatctt atgacgtaat gtttcaagag actcttctgt
    14881 tagttcttcc tctactgtct tgatgatacg agcaggattt cctgcaatca ctacattgtc
    14941 gggaaaagat ttggtcacaa ccgaacctgc gccgactact acgttgtccc ctagagtgac
    15001 acccggcgtg acgataactc ctccgcctaa ccaaacatta ttaccgattt taatgggttt
    15061 tgcgtactcc agaccgctgt tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag
    15121 ctggacattc ggagcgaaca tgcagttatc accgatttca atcgtgctga catctaaaaa
    15181 tgtacaattg aaatttgcat aaaaattctc cccaacaaat atattatatc cgtaatcaaa
    15241 attgataact ggctccatat agattttttt ccctgtacgg ccaaaagtct ctttcaacaa
    15301 ttgggatcgc agttccgctg attctgcttg gttgatgatt tggctttgac ttcttgcaaa
    15361 catacggtcg gcaaccagct caggatcatt ggcaaaataa agctcgcctg caatcatttt
    15421 ttctttttcg gttttgaatt cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa
    15481 caaccgctcc gtcttcttga ataaacgtat aaggctccgc taaataaaca ggcgtttgat
    15541 tgattactaa gattttggct gacggctggc gaaactgtac cagatcacaa aacggatgaa
    15601 cttgaaaact cgttccaaca atgacgatca gctctgcttg gctgacggca taagccgcct
    15661 gctccatgac tccatcttga aacccttcac catataacac gatgtctggg cgtatttgtc
    15721 ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt ccagtctacc gtttgttgac
    15781 attttcgaca atgacattga tacagactcc catgaaaatt gacgagttgc gggcttttag
    15841 cctttgcgtg caaaccgtcg atgttttggg aaacaaccca aacatttttt tctttcgcta
    15901 gatgtgctat ttccaaatga atgatattgg gttttgcttc aggatgataa atctttttga
    15961 caaattgata gaatttatcc ggttcattca ccattgcttg atgactcagc aagtattccg
    16021 gctgctccat cccgtggtag attcctgaca aagatcgata gtctggcact ccagaaggtg
    16081 tggatacccc tgcaccggtc aaaaatgtaa tttgctgata agctaatatc tcttttgctg
    16141 cttcgtctac tgttaattgt ttcatgtttt gtcacctcac tttccattat agcccatact
    16201 cgaacaaatt tattgttgaa aagtcagaaa aatgggaata aaaaagtgtt atgggcattt
    16261 gagcaattta tcacaatgtt cactgacaaa agaatgaaaa cgtgttacaa tactgctgta
    16321 taaaagtgag gtgaatcgat atgttaccat tctttattta ttggggcgtt attctggctt
    16381 gtatcgcctg gttagcgtta agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa
    16441 atggaaatct ttgggctttt gctttattca acgtgattgc cgcaattgtt ttagccatta
    16501 cattagccgt atatcgaaca tggggctggg gaatcaccca atactcaagt ttgatttatc
    16561 taatattagc catttatggc gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa
    16621 agaaagcggc agcttaaatg atcgagatgg aactggacac aggaaatttt ctgttctagt
    16681 tcttttttta ttgtattaca tacatttttg tgtataatta tactatcgtt tgcaatggat
    16741 agatgattca cattttgatt gttttttcgg taaaagtaaa gatttctata tttatcagta
    16801 ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt aaactttttt
    16861 tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc gagaatcatt
    16921 aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc
    16981 acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac tgtcttagca
    17041 gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa ttttagcggc
    17101 ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt accgcatttc
    17161 atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa aaaaatatgg
    17221 tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga tctctggcaa
    17281 atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct gggtatgcac
    17341 ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa ggccaaaata
    17401 gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa
    17461 aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga
    17521 tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa attgatttta
    17581 ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc aaatatgcaa
    17641 aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta aatacattcc
    17701 actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg gataatttct
    17761 cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg
    17821 aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc agcggtgcag
    17881 atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt aaaggcgacc
    17941 cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa cgaaccaaaa
    18001 ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa aaacctggca
    18061 tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta cgaggtgctg
    18121 gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt ggattactga
    18181 ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa
    18241 tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg atgatggtac
    18301 aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg aaccgaacga
    18361 ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag atcgttcgtg
    18421 gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc
    18481 atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc ttgccagcaa
    18541 caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta atcggcgtag
    18601 ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc ttccaaacat
    18661 ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga atcctgcggg
    18721 cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa atcgacaatt
    18781 caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa atcaaacatc
    18841 taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca gtcaataaag
    18901 gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc ttgcgctgta
    18961 tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa aatgtattag
    19021 ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc caatccttca
    19081 acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca actgtcctga
    19141 aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg
    19201 atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa
    19261 ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct acttcggcgt
    19321 tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca catactggct
    19381 tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct gaccgtgtca
    19441 tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc tttgtccatc
    19501 cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aaatgcttag gatcaatagc
    19561 tgattgggac aaagagctgc ggtgtttacc gcagttcttt tttatgaatt actcataaaa
    19621 ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt cagaaaactc tcttaatatg
    19681 gaagtaatta aactttttta gttaggatga tttaaaaatg aatgaagagc aagaactgca
    19741 gcgtggactg aaaaatagac atgtccaact gatttctatt ggcggagcga ttggaacggg
    19801 gttgttttta ggttccggaa agtctatcca gctagctggc ccttcgatat tgctcgccta
    19861 tcttattact ggatgcatct gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc
    19921 aaacaccaat aaccattctt ttctggattt tgttgcagaa tatcttggca aaaaagcggc
    19981 tttcatcact ggctggacgt attggttctg ctgggtatcg attgcaatgg ccgatttaac
    20041 agccatcgga atgtatgtac gctactgggc gcctggtgtt cctcagtggc ttcctgaact
    20101 gattgcatta ggtcttcttc tttgcttgaa tatggtggct gtctcattgt ttggtgagtt
    20161 agagttttgg tttgcattga tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc
    20221 ttatatgatc ctcacccact atacgacaga tattggcaca gccagtatca cgaatctatg
    20281 gtctcatggc ggattctttc caactggtgc aaaaggattt atcttagctt tccaaatggt
    20341 aacctttgct tttgctggga tagaattagt tggattagtc gctggagaaa cagaaaatcc
    20401 tgaaaaagtg ttgccggaag ctatcaataa tattcctatc aggatcatct tattttactt
    20461 aggatctctt tttgtcatta tggcaatcta tccatggaac agcttgaatc cagataacag
    20521 tccatttgta gaagtatttt cggaaattgg gatcacgatt gctgcttcct tgatcaattt
    20581 agtcgtatta agtgcagcag cttctgcctg caacagtgca atatatagta caggacgtat
    20641 gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca
    20701 tcatgtgcca gggaatgctt tgacattttc tactatcgtc attttcattt ctgttatctt
    20761 gaattacgtg atgcctagtg aagtattcac actagtaagc agtattgcca ctacttgttt
    20821 cttgtttatc tggagtatgc ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg
    20881 caaaaaagct cattcattta aaatgccgtt gtacccattt agtaattatc tggtatttct
    20941 ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt
    21001 actaacacct gtgtggttct tgcttctatt actaatctat catatgaaat atgaacgtaa
    21061 gaagtaagaa agtagttgtg acagaagtgg tcagcttcga gaaataagaa ggaatttacg
    21121 aaaattgctt cttaaatttt tgtgaaattt cggcttattt cttggagata aaagcttctg
    21181 tcccagcctc ttttctattt ccccggaaac aaccgctgat aataggcatc tgctttttta
    21241 taggaaaaaa tccaaatacc agatacaaaa aaacgtccga ataacccgat caacggtaca
    21301 aaaaagacac cggcaatcaa gcataagtta atcatcatgg agataagact tcgacggtcc
    21361 tttgacaaaa ctctcacgac atgggaatct ggtttatttg ctttcatgat ttgcctcatc
    21421 aagataccaa aagaaaaact ccacatcgtg tatactaaga cataagccat ctcaggccaa
    21481 aaagcttcag ggttacgccc gacccaacta gtaacagaag gacataaagt cagccaaaag
    21541 agcaaaaaga tattcgacca gaccactcgg taattaattc gttctgccat ctgaaatata
    21601 cgatgatggt tcacccagat caccgcaaca aatataaagc tgatggtata agctaataat
    21661 gtatttcgga tgacccataa agaatgaaag ctagatgtat tagggatctt gatctctaat
    21721 acaagaactc tcaaaacaat ggctaccact ccatcagtaa atgcttctaa tctcgacttt
    21781 gtcacgatga ttcctcctca gatgattcag cgcttttatt ttatcactta cgttattaaa
    21841 taaccatcgt tttatatagg gaattttctg aaaattgttg catatttttc gaaattttgt
    21901 tacttttaaa aaaaccgtaa aggagttatg tatgacaaaa aaattatcat ttaaagacta
    21961 tctttttata ggctccatgc ttttcggtct attttttggt gctggaaatc ttatctttcc
    22021 cgttcatttg ggtcaagaag caggcgctgc taccttttgg gcgaatctag gtttcttggt
    22081 taccggtatc ggtcttccct tcttaggggt gattgccatt ggtgtatcga aaagctcagg
    22141 tgtatatgaa ttagcaaaac ggataaatca aacatatgcg atggttttta ccatcttgct
    22201 ttacttagtt atcggaccgt tttttgcttt acctcgcctt gcaaccacat ctttcgagat
    22261 tggtttagca ccatttatcg atcctagcag acagacgcta tatctagcaa ttttcagtat
    22321 tgtatttttt gtgctggctt ggtggttttc aagaaaaccg acaaagatat tagattatgt
    22381 tggaaagttc ctgaatcctg catttttggt tttattaggt gctttgcttc tccttgcttt
    22441 tttgaatcca ctaggttctg taaatcaagc gcctattcag ccaaattatc aagagcatgc
    22501 ttttttcaca gggtttactc aaggatataa cacattagat gcgcttgctg ctttagcatt
    22561 tgggatcatc attgtcacaa caatccaaaa catgggtgta acaaaacctg ctgaaatcgc
    22621 taaagacacg atcaaatctg gtgcgatcag cattatattg atgggaatca tttatacttt
    22681 gctcgcttat ttaggtgcta tgagcttagg tagctttgct ctaagtgaaa atggcgggat
    22741 cacgctggct cagattgctg atcattattt aggtacttat ggcagtatct tattagcatt
    22801 tatcgttata ttagcttgct tgaagaccgg tattggtttg attacagctt ttgcagaaac
    22861 attcacagat ttgtttccgc aaagaaatta cgcattcttt gttgcacttg ctagtatcct
    22921 tccttgttta gcggcaaacg taggcttgac gaatatcatt caattttcgt tgcctgtttt
    22981 gatgttcatc tatccattag caatgacact gattttacta gtgttagttg gtcctttgtt
    23041 taagcagcgt acggctgttt atcgaatgac tacttatttt acattgattg cttcgatttt
    23101 tgatgggttg aacgcttgcc cagaaactat caaacaaaca cctattgttc aaaatctttt
    23161 atatgttgcg gaaagctact tgccgttttt caaattgggg atggggtgga tcgtaccagc
    23221 agttatcggt tttgttatcg gactgatttg gagtttcgct aagaaagaag aagtagcaga
    23281 ttgataaaag aggttgtgaa agagacgttc agccttgagc aataaaaagt aattttgaaa
    23341 aatggcttct cacatttttg cataaattta acttaatacc gagaaactga cttttgaaca
    23401 ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa gtattaagaa atattttgtg
    23461 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23521 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23581 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23641 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23701 aac
//
LOCUS       DAJSVP010000032        23670 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSVP010000032 DAJSVP010000000
VERSION     DAJSVP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.032"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            299..1960
                     /locus_tag="ON449_001345"
     CDS             299..1960
                     /locus_tag="ON449_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3933939.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ON449_001346"
     CDS             complement(2030..2377)
                     /locus_tag="ON449_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR3933940.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="ON449_001347"
     CDS             2461..3402
                     /locus_tag="ON449_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933941.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ON449_001348"
     CDS             3403..4446
                     /locus_tag="ON449_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3933942.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ON449_001349"
     CDS             4452..5522
                     /locus_tag="ON449_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933943.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ON449_001350"
     CDS             5512..6465
                     /locus_tag="ON449_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3933944.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ON449_001351"
     CDS             6477..7487
                     /locus_tag="ON449_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3933945.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ON449_001352"
     CDS             complement(7772..8593)
                     /locus_tag="ON449_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3933946.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ON449_001353"
     CDS             complement(8690..8968)
                     /locus_tag="ON449_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933947.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ON449_001354"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ON449_001354"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3933948.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ON449_001355"
     CDS             complement(11724..12311)
                     /locus_tag="ON449_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933949.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ON449_001356"
     CDS             12486..13808
                     /locus_tag="ON449_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3933950.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ON449_001357"
     CDS             13963..14739
                     /locus_tag="ON449_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3933951.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ON449_001358"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ON449_001358"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3933952.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ON449_001359"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ON449_001359"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3933953.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ON449_001360"
     CDS             16629..17258
                     /locus_tag="ON449_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933954.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="ON449_001361"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="ON449_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3933955.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ON449_001362"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ON449_001362"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3933956.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="ON449_001363"
     CDS             19445..19981
                     /locus_tag="ON449_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3933957.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ON449_001364"
     CDS             complement(20021..21784)
                     /locus_tag="ON449_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933958.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ON449_001365"
     CDS             complement(21787..23502)
                     /locus_tag="ON449_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3933959.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7635 a         4323 c         4802 g         6910 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga ggttttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gcttcatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat
//
LOCUS       DAJSVP010000033        23039 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSVP010000033 DAJSVP010000000
VERSION     DAJSVP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.033"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            66..2507
                     /locus_tag="ON449_001366"
     CDS             66..2507
                     /locus_tag="ON449_001366"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3933960.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2837..3541)
                     /locus_tag="ON449_001367"
     CDS             complement(2837..3541)
                     /locus_tag="ON449_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933961.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3728..3973)
                     /locus_tag="ON449_001368"
     CDS             complement(3728..3973)
                     /locus_tag="ON449_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933962.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3990..4841)
                     /locus_tag="ON449_001369"
     CDS             complement(3990..4841)
                     /locus_tag="ON449_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3933963.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5007..6011
                     /locus_tag="ON449_001370"
     CDS             5007..6011
                     /locus_tag="ON449_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3933964.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6252..6608)
                     /locus_tag="ON449_001371"
     CDS             complement(6252..6608)
                     /locus_tag="ON449_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3933965.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6976..8163
                     /gene="tuf"
                     /locus_tag="ON449_001372"
     CDS             6976..8163
                     /gene="tuf"
                     /locus_tag="ON449_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3933966.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8351..10942)
                     /gene="mprF"
                     /locus_tag="ON449_001373"
     CDS             complement(8351..10942)
                     /gene="mprF"
                     /locus_tag="ON449_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3933967.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10968..11729)
                     /locus_tag="ON449_001374"
     CDS             complement(10968..11729)
                     /locus_tag="ON449_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3933968.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12007..12528
                     /locus_tag="ON449_001375"
     CDS             12007..12528
                     /locus_tag="ON449_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR3933969.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12587..13447
                     /locus_tag="ON449_001376"
     CDS             12587..13447
                     /locus_tag="ON449_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3933970.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13632..15947
                     /locus_tag="ON449_001377"
     CDS             13632..15947
                     /locus_tag="ON449_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3933971.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16129..17970
                     /locus_tag="ON449_001378"
     CDS             16129..17970
                     /locus_tag="ON449_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3933972.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            17994..19103
                     /gene="rpoD"
                     /locus_tag="ON449_001379"
     CDS             17994..19103
                     /gene="rpoD"
                     /locus_tag="ON449_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3933973.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19323..21422
                     /locus_tag="ON449_001380"
     CDS             19323..21422
                     /locus_tag="ON449_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3933974.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21618..22481
                     /locus_tag="ON449_001381"
     CDS             21618..22481
                     /locus_tag="ON449_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3933975.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22586..>23039
                     /locus_tag="ON449_001382"
     CDS             22586..>23039
                     /locus_tag="ON449_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3933976.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7752 a         4036 c         4806 g         6445 t
ORIGIN      
        1 aactggaaca agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg
       61 cttacttgaa agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg
      121 gaagtttaag tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag
      181 ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag
      241 ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg
      301 tcccagcaat gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac
      361 gccagctgat ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt
      421 ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt
      481 ccagtatggt caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg
      541 cagagaatgt tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt
      601 cgtcccagtt gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc
      661 cagtcaagat ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta
      721 cattggaaaa actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag
      781 gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg
      841 cagaaaataa taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg
      901 acccaagtat catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag
      961 gaatgaattt atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta
     1021 tcgtagttag ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa
     1081 tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg
     1141 gattgcttcg tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag
     1201 gcttggcagt cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa
     1261 agttttatca gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga
     1321 cacaagaaac tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca
     1381 tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa
     1441 tcaatggcaa gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg
     1501 caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc
     1561 gagggcaaat cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc
     1621 atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg
     1681 ggggcggtgt tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat
     1741 ctattgattt tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc
     1801 ttgagagtgt ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta
     1861 ttttaagtaa ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac
     1921 gcttagggag aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg
     1981 aatatgcgaa gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta
     2041 tagaagctgt agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg
     2101 cctacgcggc aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata
     2161 aactagttgg aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata
     2221 tcctgccaaa agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag
     2281 cacaagtaat tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta
     2341 cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg
     2401 gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga
     2461 accagcagac agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct
     2521 ttagaagaat aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc
     2581 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
     2641 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
     2701 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
     2761 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
     2821 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
     2881 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
     2941 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
     3001 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
     3061 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
     3121 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
     3181 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
     3241 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
     3301 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
     3361 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
     3421 gtaacggcaa actataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
     3481 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
     3541 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     3601 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     3661 cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc
     3721 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
     3781 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
     3841 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
     3901 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
     3961 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
     4021 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
     4081 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
     4141 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
     4201 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
     4261 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
     4321 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
     4381 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
     4441 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
     4501 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
     4561 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
     4621 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
     4681 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     4741 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     4801 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     4861 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     4921 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     4981 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     5041 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     5101 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     5161 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     5221 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     5281 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     5341 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     5401 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     5461 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     5521 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     5581 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     5641 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     5701 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     5761 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     5821 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     5881 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     5941 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     6001 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     6061 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt gagacaaaag
     6121 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
     6181 cttcttattt cttggagtta aacgcttctg tcccagcctc tttttatcta ataaaatttt
     6241 aagaccattt actaattatc gattttttct gccgcatttt taggcacttc agaagccttt
     6301 atttcttccc agcttaatac acctttacga ggattgactt tgagtttaag atacgcattc
     6361 atctttaatg gatgttctct ttcttcgcga agcgtcacct gttttttttc accatcttta
     6421 ttataagccg cttgcttgta ggtataagtc gtgaatttct ctccgttatc tgcttgggta
     6481 gctgacattt cgccattcgt cgtgattttt gtataataag cttcgccgcc gtaaaaataa
     6541 tgataagctg caaaacttcc gccgagaaat atgcctaaaa cgacaagtac gccaattaat
     6601 tttttcatgt acttcacctc tttccttatt attaacgaaa tagaagattt cgtctattga
     6661 aagttgttgg caacagctga attttaaact tttcctcatt ttgttcttat tgcttcaaaa
     6721 gaaagttaag gactatcttg aagtttttct caaaaaacac atttttttat ttataaaagg
     6781 tacttttttc gcgtataaga cagacgagtg ctcttagttg actttgacca atattgacgt
     6841 ataataagct tagttcgaat cttacaaaaa gtaagtcgga cgtattcttt ttgatttagt
     6901 caaaaaaaga ctataataaa aaggtgagag ctgaaatttt tcagtgatct atattattta
     6961 ggaggtttgt aaattatggc aaaagaacat tatgacagaa gtaaaccaca cgttaacatt
     7021 ggtacgattg gacacgttga ccatgggaaa actactttaa ctgctgcgat cactacagta
     7081 ttaggcaaaa aaggcctagc aaatcctcaa gactatgcta gtattgatgc agctccagaa
     7141 gaacgtgaac gtgggattac gatcaatacc gcacacgtag aatatgaaac agaaaaacgc
     7201 cactacgcgc atattgatgc accaggacac gcggactatg tgaaaaacat gattacaggg
     7261 gcagcacaaa tggatggtgc aatcttagtt gtttctgcaa ctgacggtcc gatgcctcaa
     7321 acacgtgaac acattttatt gtcacgccaa gttggtgtaa aatacctgat tgttttcttg
     7381 aacaaagttg atttagtcga tgatgaagaa ttgatcgatt tggtagaaat ggaagttcgc
     7441 gagttattga gcgaatatgg tttcccaggc gatgacactc ctgtgatcaa aggttccgca
     7501 ttaaaagcat tgcaaggcga tccagatgct gaagctgcta ttatggaatt gatggataca
     7561 gtagatgaat atatcccaac accagaacgt gatacagata aaccattact attgccagtg
     7621 gaagacgtct tctcaattac aggtcgagga actgttgcct caggtcgtat tgatcgtggt
     7681 gctgttcgtg tcggtgatga ggtagagatc gtagggatca aacctgaaac acaaaaagca
     7741 gttgtaacag gtgtagaaat gttccgtaaa acgttagatt acggggaagc tggggataac
     7801 gtaggcgtgt tgttacgggg gatccaacgt gacgatatcg aacgtggaca agtacttgct
     7861 aaaccaggtt ccattactcc acatacaaaa ttcaaagcag aagtgtacgt gttgacaaaa
     7921 gaagaaggtg gacgtcatac tccattcttc aacaactatc gtccacagtt ctacttccgc
     7981 acaactgatg ttacaggaac aatcacattg ccagaagata cagaaatggt catgcctggt
     8041 gacaacgtga cgattgatgt tgacttgatc catccaatcg ctgttgaaaa cggaacgaca
     8101 ttctcaattc gtgaaggcgg acggacagta ggttcaggta tcgttacaga gatcgaagca
     8161 taagaaacgc gactcgaata ttttggtaat gaaaaatgtt taaaataaaa gagcggtcca
     8221 aacaggattt ctgtttggac cgctctttgt tcttcaattg tataagaaga gattgtagaa
     8281 gcatgaactt tcgttttact tcagtctctt ttattcatca tgagagtgtt catctatcgg
     8341 tatgcagttt ttaatacttt cttcgtatcc attttttcaa accacttatt ctaccttgtg
     8401 tctgctcaac tggcgcatta tcaataatca gcaaagcaat cataacatat gcaatccaac
     8461 tatctctaga atatagagta taacgtgaga cccagtttgt agcgtacttg cttttatatt
     8521 ctcttaatcc atggaaagaa taaaaatgag aaccaaactc gtaaaccaag tatgcaatcc
     8581 tttcctgtaa aaaactcttt ctggaagttc cgacatttga aagaggggcc atccctaaat
     8641 tgaaccattg gatatgttct tctttcatat aggcaaacaa atgcaagaac aagaaatcca
     8701 tactgccaga aggtgctttt ttagaatcat atcgcatcag atcaatcgtt cctactttat
     8761 tgttcgtgta agtaggcatg atcgttgcaa aagcgacaat ttcttcttgg gtatttttga
     8821 caactgcaat aggcgctctt tgtagataat cttcagagaa aaagcctaaa gaaaaccctt
     8881 tttcctttcg tgaacccaac caattatcag agatattttt gaaagtactc atttgctcag
     8941 tagaaaatgg tggttgtaag acatcaaatg taaatccttc tcgttcgatc cgattcaaga
     9001 cagcacgagt gcccttcatt tttttccctg aagttgtaaa actattcagg tctacataag
     9061 cttcttcccc catcttaatg aaatcgtagc cgaattcatg caaaatcata acaatctctt
     9121 cgcttgtttc gtagaaaact ggtaaatagc atagtcgatc tgtttcttcg ataaaagctt
     9181 cgattgcttc tggaaaatct tctttcttac cagaaggatc acccattaca atacatttat
     9241 tattgaagca agcgaattgg agaagaacag ttggttcacc gtctttttca taggcaaaca
     9301 tccgcttatc ttttaagaag atcaattgac tatcggagtt tcctccataa gttgtaagga
     9361 tcttcagggc cttttcttca ttaaaggctt cgccaatctg tttcttttct ccttgaagga
     9421 aatgaacaaa tagaacgatc atgaaactaa cagcgatgat ggccaacaat cccgaaaacc
     9481 aaatcttttc agaaggaaat aagaaaaagg aaataaaatg gtgacgccga tgagggaaat
     9541 ccggcagatt gtatacccca ataacgatgt aaagcaagga cagtgcacca ataatgattc
     9601 catcgatcgt acgccactcc catgaataga tcaattgttc ccgaaataat tcgtttttag
     9661 aagcaagaat gatcagaagc agaatagaca agaaaaccac cggtgtgaaa ctaaaatcac
     9721 ttaataaaac ataaagtaaa gctagaacaa tcaagaagat tgtaggcaaa tatgctcgtt
     9781 tcacacgtgc tgcgatccct ctgcccataa tgattaaaag aaatcccaat agtatactag
     9841 gaaattggat aataaaatga aatttcaatg ggttcaaact atgaagccag cgaaactctg
     9901 taaaagcttg cgggatagtg gcagaaagta ctagcatgat tccagaaaag tataataaga
     9961 caaccacgat tttatgcgca atttctgttg ctaattgttt agggatacca gaataacgct
    10021 gatcaaaagt ggaaccgata tttttgaaga aaaagacgat tccaatcaaa aacggaatga
    10081 tgtagtagaa caggcgatag agcaagatcc acacaacgac tatttctctt ggcacaccaa
    10141 tagcggacaa cccgataatc atcattacat cgaaactccc aatttctcct ggaatcatag
    10201 acacgattcc tatcactgaa gcagcaatga ataatggaat tgtttgccac agatcaatcc
    10261 ggatttccat taaataacca acagaaagga aactaaacag gacaccggtc cattccaaca
    10321 atgaaactag caatagctgt ccttgagttt taagtgaggt accaccaagg aatcctttct
    10381 ttttgaaagt agtaaataaa aagacgacag gaaaatatag accgccgccg atcaaccata
    10441 tccaatattg cttcaaaaaa ggagcaactg gcgtgaagac tacaagtaaa aaagaaagca
    10501 gactgtaaat agaaagtcca gccatcaaaa acaagaaaac tttcgataat gcttggatca
    10561 cgtttctgct ctctttcttt tgtccatata gttcagagcg aagccccaca ctaacaaacc
    10621 cgccgaaacc ggcaatattg ttgatcgtat tgatcagcca gcttgtctca aacaggtaac
    10681 gaggattctg cttttgttca agcatccgat tcaaaataat atcataacca accataggta
    10741 acacagagat aagaccaatc aatagcatta gtccgctttt ccatacaggt atagtggcaa
    10801 acgtttctcc taattgctgg atagatagag ttttaccgat ggacatcaat tcaccgacaa
    10861 taatgacaat gacagaaatc aaaaagattg ttttgaacag tcctaggtgg tttttcagcc
    10921 attgtatggt attttttaac aaataaatca ccttctattc ctaatagtta tgtttcagaa
    10981 agaaattgaa gaacttctcg attgaagcgt tcaggatctt ttctagctaa ttcatgtcct
    11041 tgttctttca caagcacaaa ggaagctttt ggtatagttt tagctatata caaagaatgt
    11101 tttagcttga ttacatcttt ttttcccacg atgatcaaag tgggggagtt gatttttttt
    11161 aaatcatttt ctgtaagccc gatgtcatgc agcaaaagtt tgataactaa aagatttttg
    11221 cgaagacttg ggcgaaataa agagagtaac catacccatg cataatgaat attactgatg
    11281 actctggcag aaaaacgaac gcctttaaca agcgtgtttc cagaatttaa aatcaatcga
    11341 tgaacttttt tagggaagct gctggcaaat accagtgcta gatttgctcc atcactaaat
    11401 ccaagaaaat ctgcctgatc gattttttcc agcaacatga tcgtattcag atcctcggcc
    11461 attaactgaa aattcaacat agatgcttcg tttgtcgaac gaccatgtcc tcggctatct
    11521 actagataca cggtatagta gcgttcgagt acagggacct gttctgaaaa aaagcggccg
    11581 ctgccgtcat tgccatgcag taaaaagaga gggaagcctt gaccgctctt ttcatagtaa
    11641 atttcgctcc catctggcat ttttgcatat ttcttttctt ttttcacata atcacctact
    11701 gtgtaaagta taacgattat atcagtcatt gggcaattgt ttttactgaa aaacaacgat
    11761 cattttcgta agatgtcaaa aaatacagga aaattcattt tttcttaagg cgtacacaga
    11821 aatcaaggaa gaaatagcca agaagataaa gaaatagtaa gataaaaaaa gatgtcaaaa
    11881 taactaggtg atgaaatgaa gcgaaaacga ttatctatta tccgtattct tttttacatg
    11941 atcattctga cggttattgg tgcggtgttg tttttatgag gaagaactta tcgttcggta
    12001 aaacgagtgg aaagctatca aatagaaata acaacagctg caaaaaatta tcatatggaa
    12061 aactacgaga atttgatcaa ggcgataata ttgaccgaat caaaaggaaa agggacagat
    12121 ctcatgcaga gctcggaaag tctgcatgga gaagctgaga tcattgataa tccagctgac
    12181 agtattgatc aaggtgtcaa atatttggcg gaaatgatag tagaagcaaa gaaacaagga
    12241 tgcgatttat ggacagctgt ccaagcatat aattttggga aagattatat tgcctatgta
    12301 aaaaatcatg gcggagaaaa cacagtagaa ttagcagaag cgtattctaa agatgtgctg
    12361 agcccacttt taggaaatga ggaagggaaa caataccgat attggcggat ccagtccttg
    12421 ctttacaatg gcggctatct ctatcataat ggaggtaaca tgttttatcc tgatattgtc
    12481 aaaatgaatg aaaaaataat agagacctat gaaaaagtct ttaagtaaaa tagcaggaaa
    12541 tgttcaatca tgaataaaat taaatgaaag aaggagtgcg caaatagtgg aaaaaaggca
    12601 aaaaatggaa aatcaagaag tggggtacac aaccaaagac tggatgttac ggtttgtgaa
    12661 gggaatgttt atcggttcag gatttatctt gccaggagtc agcggtggtg cattagcagc
    12721 tgtctttggc atgtatgagc ggctgatttc ttttttagcc caccttacaa aaaatttcaa
    12781 agaaaatgtt ttgttttttc tgcctgttgg tctaggcgga gtgacaggca tctttctact
    12841 ttctttttta gtcagttttt tattaggtgc gtatgaaaca acgatattat ggttttttgt
    12901 cggatgtatc gctggaactg ttcctgcgtt atggaaagaa gcagggaaaa aaggaagaga
    12961 tacttctgat gtgatcatca tggttgttac ttttgttctg gcttatttgt ttttattata
    13021 cggcgctcaa ttatttgacg ggcaagtcga tcagaatttt ggaacatggc tgattgccgg
    13081 cggattgatt ggtttgggta tgatcgtccc aggattgagc ccttctaatt ttttggtgta
    13141 tatgggattg tataaagcga tggctgacgg atttaaagaa gtacgacttg aagtgattgt
    13201 gccgatcgga attggtggga ttctttgtgt tcttggattg tcaaaaatca tggattacat
    13261 tttctcaaag gcatatagca agctattcca tttcatttta ggtgtagttt ttgcttcaac
    13321 gattatgatc attcctaccg attacgcggg attgggagtt gctggggtac tagcttgtcc
    13381 tgttcttttt atagccggta cagcactagg ttggtggatg agccgtttgg aggaacaata
    13441 taagtaaaga tagattgata ataaaaacag cacacgggtg attcaaacaa aaaggttggc
    13501 atcttttttg taaaatcatt cgtgtgtttt tctataaatt ttataaaaac aagttgtttt
    13561 taatcttgga acacgctaga cttaaacacg tgattatttt tgaaagttgg cggaaaatgg
    13621 agaaaaagaa aatgaatgga cttacacagc aagaggtagt tcagaaaatg gcttctggcc
    13681 agcaaaatga ctaccaggaa gatgcatcaa aaagtacaaa acaaatattc agcgataata
    13741 tattgacgtt attcaatttt ctgaactttg gtattgggat ttgtctgtta tttgtaggtg
    13801 cgtattccaa catggcgtat ttagcaatta ttgtcgtcaa tatcgttatt ggaatttatc
    13861 aagaaataca tgcaagaaat cttgtacgga aattatcgat tgtttcccaa tcagaagtac
    13921 tagtgatccg ggacggtaaa gaacaatcga tttctactaa agagctggta ttaggagatt
    13981 tggtaaaaat cggagcagga gaacagatac cagccgattt acaggttata tctggacggg
    14041 cagaagcaaa tgaagctctt ttgacaggcg aatctgattt gattgtcaaa gaaactggag
    14101 ctgaattgct ttctggaagc tttttgacaa gcggttctgt catggctgaa gttatccgag
    14161 taggtgcaga taattatgct gtccgcttga ctagcgaggc aaagactcac aaagctattc
    14221 gttctgaact tgtagattcg attcgcaaag tatccaaatt cactagtttc gtgattattc
    14281 ctttggggat catactattt ttggaagcac tgttcatccg cgatgcttct atcaaaattt
    14341 ctgttattgc atctgcagca gctttgttag gcatgcttcc aaagggtttg gtattgctaa
    14401 taagtattgc attaacgaca ggggtaacca aactggcaaa aaaacgaatc cttgttcaag
    14461 acatgtattc aattgaaacg ctggcccatg tcgatacatt atgcttggat aaaacaggga
    14521 ccattactga agggaaaatg agtatccagc gggtagaagc actacatccg ctgtacgaga
    14581 aagccatccc agaaattatg gggacgtatc tggcagaaag cagtgataac aacatcacta
    14641 tgcgggctat gagagattat tttgagcttt ctaatcggta tgatgtgaca aacaaagtcg
    14701 cattttcttc tgatcgcaaa tggggcgcga tggaatttcc agaactggga gtcgtgtacg
    14761 taggagcgcc tgaacgtttg gtggaagaag atcggcttcc ggacagtcta aaaacagccc
    14821 aagcgaatgg ctatcgcgta cttatgttgg ggatcgctcc aaatcagcaa ttgaacgaag
    14881 aaaaaccagc atttgttgag ccggttgctc tgtttgaaat cgatgatcca atccgaaaaa
    14941 acgctcaaga aacactagct tatcttcatg aggaaggtgt ggatctgaaa gtgatttctg
    15001 gagacaatcc ttttactgtc tccaatatcg ctagacgtgc aggattaccg aattatgatg
    15061 cgtatgtcga tctttctcaa attactgaag aaatggaagt gcgagaagct gctcatcgct
    15121 atacggtttt tggacgagtg agtccgcagc aaaagaaatt gctggtaaat gaattgaaag
    15181 aaagcggcag aacggttgca atgacaggcg acggtgtgaa tgatgttttg gcgttaagag
    15241 aagcagattg cagcattgct atggcagaag gtgatggagc aacacgtcag attgcgaatc
    15301 tggtattgtt ggactctgat tttactacat tgccagaagt cctgtttgaa ggacgccgag
    15361 tggtcaacaa tgtgacgaaa gtctcaggca tcttttttat caaaacgatt tattcattta
    15421 ttttatcgat tatatgtgcc gttacagcaa ttggatttcc gtttatccct atccaaatca
    15481 cgctgattga tttagcgatt gaagggtatc cttccttctt tttatctttt gaacaagata
    15541 aaagaaaaat cacttaccgt tatttgccta cagcacttat taatgcttta ccaaatgcct
    15601 tgcttgtagt agtgaatatc attgcggtgt atctacttgg aaaaacattc gcatggggaa
    15661 atgctgaaac tactactttg atgtattact tgctgattgg gatcagttgc atggcagttg
    15721 taaaagcttg ttatcccttc aacccattgc gcatcttcct agcagtaacg acgatcgtag
    15781 ggatatatgt agctgcaatg ctgttccatg gaattttgga agtaaactta gggttgggag
    15841 agagcgtagg atacttcttt gtattcatgc tcgtaaatat tgttcttcga gtattgtact
    15901 ggaaggctga tattcaaaat cagttactga aaagagtagc aaattgatgc ttaatgagat
    15961 agaaatgtgt tatacttgaa aaagcaatga aatatgatac aatcaaacat gtataattgt
    16021 gtcctttaaa atcgcactga tttcagttag tgcgattttt taccggttat tctcctgaaa
    16081 tatcttctgt taaacccaaa agatatcaat agagcagggg gtgaaaaaat ggctcaacgg
    16141 attcctcaag aggttatcga aacagtacgc agtcaaacga atatcgttga ggtgattgga
    16201 caatatgtcc agttgaaaaa gtctggtaaa aattacttgg ggctgtgtcc ttttcatgaa
    16261 gaacgtacac cgtctttttc tgttgcagaa gacaagcaga tttttcattg ctttggttgt
    16321 ggtaaaggcg gaaacgtgtt tacttttctt caagaactag aaggtctaag ttttccagaa
    16381 gcagtggtaa aagtggcaga cttcgaacag ataccgttag aagatcaatg gcgtcagcgt
    16441 actatagctg tccaaaatcc tgaatctgct acggggaaac tgattgctgc tcatgaaaaa
    16501 gcagcggaaa tctatcatca catgctattg catacaaaag ctggacagcc agctttagaa
    16561 tatttgcaaa gtcgtgggtt gtctttagaa ttaattgaag aattcaacat aggttttgca
    16621 cctaatgagc gttcttttct tcagcaagta ttcaagaatg aatcaatttc aaatgaattg
    16681 ttagaacgct ctggattgtt tgtcacgcat gatgatggcc gtatgtctga tcgtttttat
    16741 caaagagtga tgtttccact tcgtaattct caaggccaga cgatcggttt ttcaggacgc
    16801 tggcttgcac ctgaaaaagg gcaggaaaaa gatcagccca aatacttgaa tagtccagaa
    16861 acagaactgt tcaataaaag acaagtttta tttaatcttg ataaagcaag aagcgatatc
    16921 cggaaaaacg gagaaatttt gttgttcgaa gggtttatgg atgtgattgc tgcatggcag
    16981 gctggtatca aaactggatt agcttctatg gggacaagtc taacagctca acagatccaa
    17041 caaatggaaa agctgacgaa agaattggtc ttttgctatg acggagacaa cgcaggtttt
    17101 gaagcaacca atcgagggat tgaattgctt cggcaaaaca gtcgattagc actttctgtc
    17161 gtagtcgttc cagaaaagct tgatcctgat gaatatctcc gtaagtatgg agaagcatct
    17221 ttccaagaat tagtggctca tgggcgcgaa acagtcttta cttttaaatc acgttattta
    17281 aaacaaggga aaaatttaga taatgaaaaa gacaagatcg aatacctcga agaattacta
    17341 gttgaactta gccgagttgt ttcaccaatc gaacaagata tgtatctatc tcaactgtca
    17401 acagagtttc agatcggcag agagacgata caaaaacaac tgcgcgatct tcgaaggaca
    17461 aatcagcaaa atcgtcaaga acctcaggaa agtcaacaca ctcaggttac gagacaagaa
    17521 actgcacgga aaaagagacc tttgacgcaa gtagaaaagg cagagcagat tctattatat
    17581 cgtgcattta aagaagtttc tgtccgtaat attttaaaag aacgagaatt tcagtttatt
    17641 catgatgcat atgcagaagt ttatttttta ttcgatgcat atgtctcaca acattatgaa
    17701 ttcaaccttg cagagttttt agactttttg caagatgaaa atttaaggcg gttggtggtg
    17761 gaaatcgaat atttaccggt ggcagaagaa agttcgccac gagaaatcca agacttgcta
    17821 gatgtgatca gcaaatctgg cttagctgat gaaataacat taaaaaaaca aatgcagcaa
    17881 gaagccagac gaactggaaa caaacagctt gagcttgaat tgacgattga aatcatcaat
    17941 ttaacgaaac agttaaaaca agccaaatag ctactcaagg ggggccttct tttatggcaa
    18001 atggaacaga taaaaaaacg tatgaagcag cagtagcagc tttcatcaaa gaaaacaaac
    18061 ccaaacgaca agttgtatat gatgatttat ccaatcagct tgctacacca ttcactttga
    18121 atgcagatga aatggacaaa ttgatccaaa agattgaaga cgcaggtatc agtgtggtgg
    18181 atgaaaatgg tgatccatca attcacagct tgaagagtgc tgagaaaaaa gctgaacaag
    18241 caaaaacaga agatctttct gcgccaaccg gtgtaaaaat caatgacccg gttcgtatgt
    18301 atctgaaaga aattggccgt gtctcccttt taactgcgga agaagaagta gcgttagcat
    18361 tgaaaatcga agaaggcgac caagaagcga aacagcgtct ggcagaagca aaccttcgtt
    18421 tagtagtaag tatcgctaaa cgttatgtag gacgaggcat gcagttcctt gacttgatcc
    18481 aagaaggaaa catgggactg atgaaggcag ttgaaaaatt tgactaccgc aaaggattta
    18541 agttttcaac ctatgctaca tggtggattc gtcaagccat cactcgagca atcgctgacc
    18601 aagcacgtac gatccgtatt ccagttcata tggttgaaac aattaataaa ttgatacgta
    18661 tccaacgtca attgctgcaa gaccttggaa gagaacctac gccagaagaa atcggtgcgg
    18721 aaatggatct tccaacagaa aaagtccgag aaatcttgaa aatcgcgcaa gaacctgttt
    18781 cccttgaaac gccaatcggt gaagaagatg actctcactt aggtgatttc atagaagacc
    18841 aggaagcaac aagtccagca gaacacgcag cttatgagtt actaaaagaa caattggaag
    18901 atgtactaga tacattgact gatcgggaag agaatgtcct tcgcttacgt tttggtttag
    18961 atgatggccg gacgagaaca ttggaagaag tagggaaagt atttggtgtt actcgggaac
    19021 gtattcgtca gatcgaggct aaagcattaa gaaaattgcg ccatccttct cgttcaaaac
    19081 aattaaaaga tttcttagaa taagcgtgga ctattgacca gaagagaaat cagtaaaact
    19141 gtttggttta ttttaaaaga aaaaaagctt gagactacgc agtctcaagc ttttttttct
    19201 ataggaagga aattcattca ttatttttaa gtaaatccta aaaataagct attggcgtga
    19261 cgtatagaat atgatagatt aaatggtagc gtaaacctag taaaggagcc gatcgctaag
    19321 tgatgaaaaa atgtccaaat tgcggatcga aaaaaatgga gcagaatact atctgtccag
    19381 actgcggtca tcaaataact gcagaagatc agcaaataaa agaatcaatt gaagaaacaa
    19441 atacagatgc agctatgcag gataaatcaa ctgaagatgc agtctttcct gatacagaat
    19501 taaacgatcc aatcgaatgg tcggaattga aagatttacc tctagaatct gtaatggaat
    19561 tattcgaaaa agcagaacca gaagacgaag aaccacctgc tcacgatcaa gtaactaata
    19621 tagagaaaaa tgaacagaag aaagcaactg ttcatgagac ttcggaagaa tcaggaaaga
    19681 atcaagaaga aacgcttgaa tcaaaacaaa aaagaagagt agctgaactg aaagaaatcg
    19741 tcgataatga ggaagaaaat tcgatactat ccgcttatat caaagcacat cgtgaagata
    19801 caaaagaaga gcacgcgaaa gagctgctca aaatgatcag tgaaaaaatt gcacaggaaa
    19861 acaaagtaga gccagaaaag gaacaagaaa ctgaaaaaga ctctgataga gaaaacgcca
    19921 caggtaaaga aacagtaaaa ataaaagacg taaaaacgga tatcacagaa aaaactgaag
    19981 tatttactga aaaacaatta gaaaaggaaa aaacgaataa actgccggaa gcagagaaaa
    20041 aagaaatact cgaaaacaca gacaaaaagt caacagagcc cgatgaaata aatagtgaat
    20101 ccgatgctga aaatcaatct gtaagcaaag agggtactga tcttgaagca aaaatagaaa
    20161 aagaagagaa ccagcctaag ctgtttgaag cagaagcgaa atcaataaca atcgtgccag
    20221 atactgaaaa aacacatcaa gaatcaaact tagaaaaaac agatccagat aaagcgcaaa
    20281 agaaaaaatc aaaaaaagta ccttatctcg ttttagcagc agtacttcta ctcggtggag
    20341 gaagctgggc ttattatgac catcatcaaa aagtagaagc cgaagtagca gcagaagcaa
    20401 aaagacagca acaaatggaa gaacttcatg agaaacttgc tgccttttat acagatgatc
    20461 accatcaatt tattcgtgca gatatgattc atcaagattt atcaaaacta aaaaatgatt
    20521 tatccagtct taagaatgaa aaaggatatt cagagttaga aacaacttac caagatatcc
    20581 aatctaagat ccgagatatc caaaaagtga atgagtggtt catagcacct gccatcgtcg
    20641 atgatcatct agcagatgac atcaagttga aagcagataa agagatccaa gcaatcgaaa
    20701 cggataacac agattttgga aaattgattc aacaagcaca aacagaagct aaaaatcaat
    20761 atgctgaatt gcaaacagct aaagaaaaaa cagctatagt ctttaaagac ggaaaagtag
    20821 tggattcagc aacacgcgac caatacaaga cggcaaaaga agcagtagaa aaagtgaaaa
    20881 atacagatct atctgctcag ttgaatgaac aattgaaaca agtagataag atgctcactg
    20941 ataaagagaa aaaagaagaa gaggaaaaga aaaaagcaga agaagcgaaa aaagcagctg
    21001 aagaacaagc aaaacaagcc gctcaagcag cagctgcagc tcaagccgct caacaagctg
    21061 cggtcgcaca agcagcacag caagaagcgg catcctcgac cccttcctct tcaaactcag
    21121 cgaatcagcc aatcaaatct gccagagcaa gtgatattgc cgatgcatct aatccagcat
    21181 ggacgtgggc accaggtgtc caaagcaaag tgattgctac atgtatccaa cgtggatata
    21241 tcgttgaagg aggttacaga ttagaaaaag ccagaatcga aaatggggaa ggctattata
    21301 atctatatgc cacttcaaca aaatctgcat tgatgaaagg cattggagaa agtgctttgc
    21361 cattttacat cgtaaccatc aactgtaaaa ctggatggtt cggaggaaat gggagcaatt
    21421 aattttaggc aaatgaaaga ggctgtgagc ctgccgttta acaccgttta ttcgaaaaag
    21481 gggagtatga cacaactgtt gtcatactcc ttttttcacc tttccaaacc aaggctcagc
    21541 tattcaagta gataacatta tgttatgata gcaaagaaat gtattaagaa aacctttcac
    21601 tatagaggga gaaaaaaatg aacgatttat tagcaactat atttacaggt attattattg
    21661 atgaaaatga aaaaaactac tttgtccaaa aaaacggaca aacctttggc ttaagcaaat
    21721 cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt cggttaccaa aatcaaaaac
    21781 atgaaaatcg gatcactaca caaattccta aaagccgtat cggtcattac gcttttggaa
    21841 tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga tatcggtctt ccagataaag
    21901 actttgtcgt atctcttgat gaattgccga caatgacaga gctatggcct aaaaaagggg
    21961 atcgcttgat gatttccctt agagttgatg caaaagaccg tatttggggc agtttagcag
    22021 atgaaaaaat attcaaatct ttaagcaaat atggaacaga agaaatgaaa aacaaagatg
    22081 tgataggaac agcataccgc ttgaaattaa caggtacgta cgttttaacg gaagatttct
    22141 atcttgggtt tatccatcct tctgaacgat ataatgaacc tcgtctaggc gagcagataa
    22201 aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa tctttcattg aagccaagag
    22261 catatgaagc tatatcagat gatgcacaaa tgattttaac cattttagaa cgttctgcag
    22321 atcagcaaat caactttaca gataaatcgg atccagaaga aatcatgaaa gcttttggta
    22381 tcagcaaagg agcatttaag cgcgcattag gtaatctttt gaaacaagga ttgattacac
    22441 aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg agaatccctt gcatgttttt
    22501 cttaactagc ttataataat tttaattaac atagtgttat atcttttttt atttgtaatt
    22561 attataaatt gttggggttg agtaaatggt catgaatgct atttcggcga tgaaaaaaac
    22621 aaaacagcag ctacatgaag cggggtttaa gctgacaccg caaagagaag ctactctgct
    22681 tgtgcttttg gaaaatgaga aagagcattt atctgcagaa gaaatcttct ttttagtgaa
    22741 aaaaaagagt ccggaaattg gacttgctac tgtttatcga acccttgaaa tcttgacaga
    22801 attgaaaatt gtcgataaag taagttttaa tgatggatta gcccgttatg atttgagaaa
    22861 agaaggcgcc aaacatttcc atcatcattt actatgcttg aactgcggca cgatcgagga
    22921 agtagaagag gatcttttac ttgaagtgga gcaagtagta gaagaacgtt atcatttttt
    22981 agtaaaagat catcggctaa cgttccatgg aatctgtcag gaatgctatg aaaaagaaa
//
LOCUS       DAJSVP010000034        22128 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSVP010000034 DAJSVP010000000
VERSION     DAJSVP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.034"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(145..663)
                     /locus_tag="ON449_001383"
     CDS             complement(145..663)
                     /locus_tag="ON449_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3933977.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(665..1282)
                     /locus_tag="ON449_001384"
     CDS             complement(665..1282)
                     /locus_tag="ON449_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3933978.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1279..2133)
                     /locus_tag="ON449_001385"
     CDS             complement(1279..2133)
                     /locus_tag="ON449_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3933979.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2130..3041)
                     /locus_tag="ON449_001386"
     CDS             complement(2130..3041)
                     /locus_tag="ON449_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3933980.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3082..4416)
                     /locus_tag="ON449_001387"
     CDS             complement(3082..4416)
                     /locus_tag="ON449_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3933981.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4624..5454
                     /locus_tag="ON449_001388"
     CDS             4624..5454
                     /locus_tag="ON449_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3933982.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5698..5980)
                     /locus_tag="ON449_001389"
                     /pseudo
     CDS             complement(5698..5980)
                     /locus_tag="ON449_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6138..6332)
                     /locus_tag="ON449_001390"
     CDS             complement(6138..6332)
                     /locus_tag="ON449_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933983.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6837..7022)
                     /locus_tag="ON449_001391"
     CDS             complement(6837..7022)
                     /locus_tag="ON449_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933984.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7872..8213)
                     /locus_tag="ON449_001392"
     CDS             complement(7872..8213)
                     /locus_tag="ON449_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3933985.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8213..8443)
                     /locus_tag="ON449_001393"
     CDS             complement(8213..8443)
                     /locus_tag="ON449_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3933986.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8730..9068
                     /locus_tag="ON449_001394"
     CDS             8730..9068
                     /locus_tag="ON449_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933987.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9273..9848)
                     /locus_tag="ON449_001395"
     CDS             complement(9273..9848)
                     /locus_tag="ON449_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3933988.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(10457..11038)
                     /locus_tag="ON449_001396"
     CDS             complement(10457..11038)
                     /locus_tag="ON449_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3933989.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11221..11847)
                     /locus_tag="ON449_001397"
     CDS             complement(11221..11847)
                     /locus_tag="ON449_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3933990.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11869..13200)
                     /locus_tag="ON449_001398"
     CDS             complement(11869..13200)
                     /locus_tag="ON449_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3933991.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13355..13756)
                     /locus_tag="ON449_001399"
     CDS             complement(13355..13756)
                     /locus_tag="ON449_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3933992.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13842..14048)
                     /locus_tag="ON449_001400"
     CDS             complement(13842..14048)
                     /locus_tag="ON449_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3933993.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14143..15645)
                     /locus_tag="ON449_001401"
     CDS             complement(14143..15645)
                     /locus_tag="ON449_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3933994.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15663..16118)
                     /gene="argR"
                     /locus_tag="ON449_001402"
     CDS             complement(15663..16118)
                     /gene="argR"
                     /locus_tag="ON449_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3933995.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16216..16833)
                     /locus_tag="ON449_001403"
     CDS             complement(16216..16833)
                     /locus_tag="ON449_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3933996.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17054..18427
                     /locus_tag="ON449_001404"
     CDS             17054..18427
                     /locus_tag="ON449_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3933997.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18502..19614
                     /locus_tag="ON449_001405"
     CDS             18502..19614
                     /locus_tag="ON449_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3933998.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(19686..21323)
                     /locus_tag="ON449_001406"
     CDS             complement(19686..21323)
                     /locus_tag="ON449_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR3933999.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(21491..22099)
                     /locus_tag="ON449_001407"
     CDS             complement(21491..22099)
                     /locus_tag="ON449_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3934000.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6455 a         4505 c         3858 g         7310 t
ORIGIN      
        1 ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt aagacaaatg
       61 aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac
      121 tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact
      181 tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga
      241 ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa
      301 caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt
      361 cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt
      421 cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc
      481 tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga gtaaccaaat
      541 aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt
      601 tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa
      661 catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt
      721 gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga
      781 atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa
      841 aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt
      901 tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat
      961 agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat
     1021 aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc
     1081 agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc atatacagaa
     1141 ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct
     1201 gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt
     1261 taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa
     1321 ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc
     1381 catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa
     1441 cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc
     1501 gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg
     1561 actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt
     1621 gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg
     1681 aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg
     1741 gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt
     1801 tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt
     1861 accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa
     1921 ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg
     1981 ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat
     2041 gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt ttgtttgccg
     2101 attcacactg gtctttttga aataatccat catttgccgt cacctccaga ataatagacc
     2161 cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc
     2221 attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc
     2281 agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa
     2341 gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata
     2401 ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt
     2461 tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt
     2521 gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca
     2581 aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca
     2641 gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt
     2701 aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct
     2761 aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg
     2821 tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca
     2881 atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc
     2941 ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc
     3001 catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt
     3061 acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg
     3121 tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc
     3181 attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt
     3241 aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt
     3301 cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt
     3361 ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt
     3421 tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc
     3481 ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa
     3541 actagagtaa taattgatat tttgggcacg catggctact aatccggaaa taaatggatc
     3601 agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc
     3661 ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt
     3721 ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc
     3781 taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag
     3841 tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc
     3901 agcttcttta aataactttt tattataaaa aatgacttgt gtatatgtat tgaatgggat
     3961 cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga
     4021 agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc
     4081 aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa
     4141 agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg
     4201 tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca
     4261 aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc
     4321 acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga
     4381 acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta
     4441 accgatgtcg gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt
     4501 atgattctaa aaaaattatt aatgatacca atactttata taattcgcaa gcgttttctt
     4561 tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt
     4621 ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca
     4681 ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct
     4741 atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg
     4801 atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt
     4861 ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc
     4921 ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt
     4981 cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg
     5041 ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt
     5101 atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga
     5161 gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt
     5221 tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac
     5281 ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca
     5341 ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca
     5401 aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct
     5461 acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca
     5521 ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg
     5581 gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag
     5641 ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta
     5701 ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt
     5761 tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg
     5821 tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg
     5881 ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt
     5941 tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag
     6001 tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa
     6061 acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc
     6121 tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt
     6181 ccaagaaata aagccgtata aaaaccagaa cctatgaaat ctttatttat tagatattca
     6241 acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc
     6301 tttttataga atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt
     6361 aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc
     6421 ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta
     6481 cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc
     6541 atccagtata cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat
     6601 ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga
     6661 taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct
     6721 caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg
     6781 aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat
     6841 atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact tggaactaat
     6901 gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt tagcagccca
     6961 accaacatat accctataaa agctaataag atggttagga atcctttaga atttttactc
     7021 attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat aataatattg
     7081 acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata
     7141 gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt
     7201 atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt acattcataa
     7261 ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat
     7321 tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc
     7381 tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct
     7441 tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca
     7501 gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca
     7561 tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct
     7621 gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt
     7681 gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat
     7741 atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga
     7801 catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt
     7861 acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat
     7921 ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta
     7981 ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc
     8041 tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa
     8101 cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa
     8161 tcaatccata caatatcacc ttttttagga atataaattc tttcttctga cactataaaa
     8221 tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt
     8281 ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat
     8341 acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta
     8401 ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa
     8461 taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa
     8521 attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt agccagttca
     8581 ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac
     8641 ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga
     8701 aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt
     8761 ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt
     8821 caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa
     8881 ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga
     8941 ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca
     9001 gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag
     9061 agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa
     9121 caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc
     9181 ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt
     9241 tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc
     9301 atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt
     9361 tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca
     9421 gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca
     9481 atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca
     9541 taaaagccac tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt
     9601 ttttcttcta ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta
     9661 aatccccact tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca
     9721 acaagatttg acggaaaaac ttccccagct gctaattctg ctgcctgctt cggttgtttc
     9781 gtctgggctt ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga
     9841 ccacacatcc taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc
     9901 accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct
     9961 ccagtgaatg gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
    10021 gagaagctgc tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc
    10081 tctgggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    10141 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
    10201 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
    10261 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
    10321 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
    10381 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    10441 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
    10501 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
    10561 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
    10621 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
    10681 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
    10741 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
    10801 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
    10861 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
    10921 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
    10981 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
    11041 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
    11101 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
    11161 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
    11221 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
    11281 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
    11341 cacaacattg aatccactct ctatcaattc acgcatatgg gaatcgctac acaagtttcc
    11401 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
    11461 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
    11521 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
    11581 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
    11641 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
    11701 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
    11761 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
    11821 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
    11881 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
    11941 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
    12001 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
    12061 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
    12121 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
    12181 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
    12241 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
    12301 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
    12361 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
    12421 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
    12481 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
    12541 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
    12601 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
    12661 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
    12721 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
    12781 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
    12841 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
    12901 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
    12961 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
    13021 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
    13081 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
    13141 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
    13201 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
    13261 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
    13321 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
    13381 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
    13441 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
    13501 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
    13561 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
    13621 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
    13681 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
    13741 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
    13801 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
    13861 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
    13921 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
    13981 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
    14041 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
    14101 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
    14161 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
    14221 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
    14281 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
    14341 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
    14401 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
    14461 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
    14521 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
    14581 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
    14641 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
    14701 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
    14761 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
    14821 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
    14881 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
    14941 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
    15001 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
    15061 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
    15121 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
    15181 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
    15241 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
    15301 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
    15361 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
    15421 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
    15481 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
    15541 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
    15601 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
    15661 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
    15721 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
    15781 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
    15841 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
    15901 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
    15961 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
    16021 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
    16081 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
    16141 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
    16201 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
    16261 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
    16321 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgcaggtt cttcggcata
    16381 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
    16441 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
    16501 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
    16561 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
    16621 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
    16681 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
    16741 gatgctaaag aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata
    16801 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
    16861 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    16921 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    16981 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    17041 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    17101 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    17161 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    17221 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    17281 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    17341 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    17401 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    17461 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    17521 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    17581 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    17641 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    17701 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    17761 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    17821 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    17881 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    17941 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    18001 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    18061 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    18121 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    18181 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    18241 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    18301 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    18361 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    18421 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    18481 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    18541 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    18601 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    18661 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    18721 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    18781 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    18841 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    18901 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    18961 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    19021 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    19081 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    19141 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    19201 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    19261 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    19321 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    19381 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttt
    19441 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    19501 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct
    19561 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    19621 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    19681 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctagcaag
    19741 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    19801 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    19861 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    19921 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    19981 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    20041 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    20101 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    20161 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    20221 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    20281 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    20341 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    20401 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    20461 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    20521 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    20581 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    20641 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    20701 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    20761 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    20821 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    20881 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    20941 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    21001 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    21061 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    21121 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    21181 gctagttgat aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc
    21241 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    21301 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    21361 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    21421 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    21481 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    21541 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    21601 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    21661 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    21721 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    21781 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    21841 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    21901 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    21961 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    22021 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    22081 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gtttttta
//
LOCUS       DAJSVP010000035        22112 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSVP010000035 DAJSVP010000000
VERSION     DAJSVP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.035"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..369
                     /gene="yaaA"
                     /locus_tag="ON449_001408"
     CDS             127..369
                     /gene="yaaA"
                     /locus_tag="ON449_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3934001.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            356..1480
                     /gene="recF"
                     /locus_tag="ON449_001409"
     CDS             356..1480
                     /gene="recF"
                     /locus_tag="ON449_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3934002.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1477..3423
                     /gene="gyrB"
                     /locus_tag="ON449_001410"
     CDS             1477..3423
                     /gene="gyrB"
                     /locus_tag="ON449_001410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3934003.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3443..5914
                     /gene="gyrA"
                     /locus_tag="ON449_001411"
     CDS             3443..5914
                     /gene="gyrA"
                     /locus_tag="ON449_001411"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3934004.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6150..6449
                     /locus_tag="ON449_001412"
     CDS             6150..6449
                     /locus_tag="ON449_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3934005.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6499..7011
                     /gene="ssb"
                     /locus_tag="ON449_001413"
     CDS             6499..7011
                     /gene="ssb"
                     /locus_tag="ON449_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3934006.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7037..7273
                     /locus_tag="ON449_001414"
     CDS             7037..7273
                     /locus_tag="ON449_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3934007.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7416..9389
                     /locus_tag="ON449_001415"
     CDS             7416..9389
                     /locus_tag="ON449_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3934008.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9395..9847
                     /gene="rplI"
                     /locus_tag="ON449_001416"
     CDS             9395..9847
                     /gene="rplI"
                     /locus_tag="ON449_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3934009.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10121..11488
                     /gene="dnaB"
                     /locus_tag="ON449_001417"
     CDS             10121..11488
                     /gene="dnaB"
                     /locus_tag="ON449_001417"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3934010.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12268..13560
                     /locus_tag="ON449_001418"
     CDS             12268..13560
                     /locus_tag="ON449_001418"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3934011.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14347..15210
                     /locus_tag="ON449_001419"
     CDS             14347..15210
                     /locus_tag="ON449_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3934012.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15339..16961)
                     /locus_tag="ON449_001420"
     CDS             complement(15339..16961)
                     /locus_tag="ON449_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3934013.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17210..17731
                     /locus_tag="ON449_001421"
     CDS             17210..17731
                     /locus_tag="ON449_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3934014.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17917..19647)
                     /gene="spxB"
                     /locus_tag="ON449_001422"
     CDS             complement(17917..19647)
                     /gene="spxB"
                     /locus_tag="ON449_001422"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3934015.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19812..20630
                     /locus_tag="ON449_001423"
     CDS             19812..20630
                     /locus_tag="ON449_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3934016.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20733..20930)
                     /locus_tag="ON449_001424"
     CDS             complement(20733..20930)
                     /locus_tag="ON449_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934017.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21099..21677
                     /locus_tag="ON449_001425"
     CDS             21099..21677
                     /locus_tag="ON449_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3934018.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21772..21909
                     /locus_tag="ON449_001426"
     CDS             21772..21909
                     /locus_tag="ON449_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934019.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7091 a         3991 c         4669 g         6361 t
ORIGIN      
        1 tttgtttaaa tcaccttttt tagattaaat cgtcttatat ggaaaaatat atggataaat
       61 ttgttttatt gcgaaaaaaa aggtataata gagtatactt ttaataatga aaatgaggga
      121 tgggcattga aacaacaagt cattctaaat acggaattca tgacgttggg ccaaatgttg
      181 aaagaagtca cagttatcgg tagtggcgga caagcaaaat ggtatttggc agaaaacact
      241 gtgttagtcg atggcgaacc agaaaatcgt cgtggacgca aactttatcc cggcatgatg
      301 gttgaagttc cagaagtagg aacttttttt atggtaaaaa aaggaaacga cgataatgag
      361 gctgaataag ctgtatttaa aaaattatcg aaattacgaa gagctgaaca tcgaattttc
      421 caagaacctc gtcatctttt taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag
      481 tatctacgta ttagctatga cgcgaagtca tcggacaagt agtgagcagg aattgattgg
      541 ctggactgac gatcaggcga tgattcaagg agagatcaca aaaggttcat ccactcttcc
      601 attagaaatc ttattatcta aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa
      661 aaagttaagt acttacgtgg gacaaatgaa tgtcatttta tttgcaccag aagatctttc
      721 tttagtaaaa ggcagcccgc agatcaggcg gaagttttta gatatggaat taggccagat
      781 caatccaatc tatctttatg atcttgtcca ataccaagca attttgaaac aaagaaacca
      841 gtacttgaag caattgaatg aaaaaaaaca aactgatttg ctttatttgg atattttgtc
      901 cgaacaacta gcagcgtttg gcagcaaagt attgaaagca agagcacagt tcgtcaagcg
      961 attggagttt tgggctaatt ctttgcatca gcaaatcacc catcaaaaag agcagctgga
     1021 aatcgagtat ctcaccgcag tcgattcttt agaaacacat acgcaagagc aaatccaaga
     1081 acaatttctc gcacttttga accaaaataa aaagaaagac ctttttcggg gaacaacgac
     1141 agttggtcct catcgtgacg atctttcctt ttttattaat cagaaaaatg tgcagactta
     1201 tggatcgcaa ggacagcaaa gaacaactgc actaagtgtc aaattagcag aaatcgattt
     1261 gattaaagaa gagacagggg aatatccaat ccttttgtta gatgatgtga tgagcgagct
     1321 ggatgataat cgtcaactcc atctgctaga aacaatcgaa ggaaaagttc aaacgttctt
     1381 aacaacaacg acacttgacc atgtaaaaaa taaaatgact gttgaacctg agatttttta
     1441 tgttcaacaa gggaaactaa aaggagaaga aactgaatga cagaagaaag aagtttagta
     1501 gaacgcgcta aagagtatga tgccagtcag attcaagtac ttgaaggact tgaagccgtt
     1561 cgtaaacgac ctggcatgta tatcggttcg actagttctg agggtctgca tcaccttgtt
     1621 tgggaaattg tcgataattc gatcgatgaa gtattagcag gttttgcaac gaaaatccat
     1681 gtgattattg aaaaagacaa cagtatcacc gttattgacg acggtcgggg gatacctgta
     1741 gatatccagg caaaaactgg acgtcctgca gttgaaacgg tctttactgt tttacatgct
     1801 ggaggaaaat tcggcggtgg tgggtacaaa gtttctggtg gacttcacgg agtaggatct
     1861 tctgttgtaa acgcgctttc tacacagctt gatgtcaaag tatacaaaga aggaaatgtg
     1921 tactatcaag aatatcgtcg tggagctgta gtggacgact taaagattat cgaacaaacg
     1981 gatcgccatg gaacaacggt tcactttacg ccagaccctg aaatttttac agagacaact
     2041 gagtttgatt tttcaaaatt ggctacccgg atccgtgaac ttgctttctt gaaccgcggg
     2101 atgcgtatat cgattgaaga caaacgagaa gaagaaccag tcatcaatga ataccattat
     2161 gacggcggga tcaaaagtta tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca
     2221 gaacctgtat atttggaagg tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat
     2281 acagacggct accattccaa tatcatgagt tttgccaaca atatccatac gtatgaaggt
     2341 ggaacacatg aatcaggatt caagactgct ttaactcgtg tgataaatga ttatgcccga
     2401 aaacaaaagc tgatgaaaga aaatgacgac aacttgaccg gtgaagacgt gcgagaagga
     2461 ttgacagctg ttatttctat caagcatcct gaccctcaat tcgaaggaca aacaaaaaca
     2521 aaattgggca attccgaagt acggacagtc actgatcggt tatttagcga acatttcatg
     2581 aaattcttgc tggaaaaccc aagtgtgggc cggcaaattg ttgaaaaagg attactagct
     2641 tccagagctc gtctagcagc gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta
     2701 gagattagta atctgccagg aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc
     2761 gaattgttca tcgtcgaagg agattcggcc ggcggatcag ctaagcaagg tcgtaatcga
     2821 gagtttcaag ccatcttgcc tattcgtggg aaaatcttga atgttgaaaa agcaagcatg
     2881 gacaagatct tggcaaatga agagatccgg tctttattta ctgctatggg tacagggttt
     2941 ggagaagatt ttgatgtatc gaaagctcgc taccacaaat tagttatcat gactgatgcc
     3001 gatgtcgacg gcgcgcatat ccgtacattg ctgctgactc ttttttatcg ttacatgcgt
     3061 cctattgttg aagctggata cgtctatatt gctcagccac ctttatacgg ggtaaaacaa
     3121 gggaagaata ttacgtatgt acaaccagga aaaaatgcag aagaagagtt gaaacaagtc
     3181 gttgcttcac ttcctgcctc accaaaacca agcgttcagc gttataaagg acttggagaa
     3241 atggatgatc atcaactatg ggaaacaact atggacccaa gcaatcggat gatggcccgc
     3301 gtcagtgtgg acgatgctat tgcagcagat cagatatttg aaatgttgat gggggatcga
     3361 gtagaacccc gtcgtgcatt catcgaggaa aatgcacact atgtgaagaa cttagatatt
     3421 taatcatgaa ggaggaaaaa gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa
     3481 tctaaccagc gaaatgaaag actccttcat cgattatgcg atgagtgtta ttgtcgcacg
     3541 tgcacttcct gatgtcagag atggtctgaa acccgttcat cgccgtattt tatacggcat
     3601 gaacgaatta ggtgtaacac cggataaagc ccataaaaaa tctgccagaa tcgtcggaga
     3661 tgtcatgggt aaataccatc ctcatggaga cattgcgatc tatgagtcaa tggtacggat
     3721 ggcacagcca ttcagctacc gttatatgct agtggatggt catggaaact ttggttctgt
     3781 tgacggtgac ggtgccgccg ctatgcgtta taccgaagca cggatgagca aaatcgctac
     3841 agaaatgctg cgtgatatca ataagaacac tgttgatttc caaagcaact atgatgatac
     3901 agaaaaagaa ccagttgtac tacctgctcg cttcccgaat cttttagtga acggaacaac
     3961 ggggatcgcc gtaggtatgg ccacgaatat cccacctcat aacttgtctg aggtagtagc
     4021 ggctatcgat ttgctgatgg ataatcctga tgtgacgacc aatgaattga tggaagtatt
     4081 acctggacca gattttccaa caggtggttt ggttatgggg aaatcaggaa tccgaagagc
     4141 gtatgaaaca ggtaagggtt ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc
     4201 aaatggtaaa gaacggatca tcgtgacaga attgccttat atggtcaata aagcaaaatt
     4261 gatcgaacgg atttctgaac tgcacagaga caagcgtatc gaagggatca cagatttgcg
     4321 ggacgaatct tcccgtgaag gtatgcggat cgtcattgac atccgtcgtg acgccagcgc
     4381 atccgttatc ttgaataact tatataaatt gacatccttg caaaattcat ttggattcaa
     4441 tatgttggcg atcgaaaaag gtgtgccgaa agttttgagc ttgaaacaaa tccttgaaaa
     4501 ctatatcgaa catcaacgcg aagtcatcac acgacggacg gtctttgaaa aagaaaaagc
     4561 agaagcaaga gcacacatct tagaaggttt gcgtattgct ttggatcata tcgatgaaat
     4621 cattgcgatt atccgcgggt ctaaatcaga tgacgaggca aaagcaacaa tgatcgaacg
     4681 gttcgattta tccgatcgcc agtctcaagc tattttagat atgcgtttac gccgattgac
     4741 cggtttggaa cgggaaaaaa tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga
     4801 tttaactgac attctggcaa gaccagaacg tgttacagaa atcatcaaga atgaattagc
     4861 agacttgaat caacgatttg gtgataaacg acgcacagaa ttactagtcg gtgaggtctt
     4921 aagtctggaa gatgaagatt taatcgaaga agaagaaatc gttattacat tgacaaacaa
     4981 tggttacatc aaacgtttgg caaataatga attcagagct caacgaaggg gtggacgtgg
     5041 tgtccaagga atgggtatcc acaatgacga cttcgtcaaa aatctggttt cttgttctac
     5101 ccatgataca ttgttattct ttacgaataa cggaaaagtg tatcgggcaa aaggatacga
     5161 aattccagaa tacgggcgta cagccaaagg aatcccaatc atcaatttac taggtatcga
     5221 ttcgagtgaa agcatccagg caatcattgc ggtgacggga gaagcagaag aaggccacta
     5281 tctcttcttt acgacgcgtc aaggtacagt aaaacgtact tctgtcactg cttttgctaa
     5341 catccgaagt aatggtctga tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt
     5401 gctactgaca gatggtcaat ctaatatcat tattggaaca cataacggct actctgtcac
     5461 tttcaaagaa gaagcagtcc gcgatatggg acgtacagct tccggggtca gagggatacg
     5521 cctgcgtgag gaagattatg tcgttggggc agccttaatg aaggaaaatc aagaagtatt
     5581 agtgatcaca gaaaaaggtt acggaaaacg gacaaaagta tcggaatatc cagtgaaagg
     5641 ccgcggaggt aaagggatca aaacggcgaa catcactgaa aaaaatggtc cactagctgg
     5701 attgaccact gtttctggag atgaagacat catgctgatt acagataaag gtgtcattat
     5761 ccgtttcaac gtatcgactg tttctcaaac tggacgttca acattaggcg tacgtttgat
     5821 gaagatggaa gctgatacga aagttgtcac gatggcagca gtcgaaccag aagctgcaga
     5881 acaaacagtt gaagaacaag gatcagaaga ataaccatca agactaagtg tgtaagctag
     5941 caagaataac cgattaaaaa atctggttat tcctgctggc ttttttgatt agttgaattt
     6001 ctcttaaaat attcgaaact gttcattctg tattgctttt gaccaaaata gagtgtataa
     6061 ttagagcttg tgagtagtct aattaattag ataactctcc ttgctcttag gaacctaaga
     6121 gccgaaagtc cacagggagg tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca
     6181 ttattcgtcc aaacattgat gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa
     6241 tcttgaaaga taacggagca gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg
     6301 cttatgaaat gaacggttac cgtgaaggta tctatcacat cgttaaagta tcttctccat
     6361 catcagcaaa tgctgtcaat gaatttgacc gtcttgctaa aatcaacgac gatatcattc
     6421 gtcacatgat tgttaaagaa gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga
     6481 tgagaggaga tgactatttt gattaataat gttgtattag ttggacgtct gacgaaagat
     6541 ccagatttgc gttacacagc aagtggtacg gcagtcgcaa catttacttt ggctgtgaat
     6601 cgtaatttta caaaccaaag cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg
     6661 cgcaaatcag cagaaacttt agcaaactat gctcgtaaag gaacactttt aggtgttact
     6721 ggacgtatcc aaactcgttc ttatgataat cagcaaggac aacgtgtata cgtgacagaa
     6781 gtagtagctg acaacttcca attgctggaa agtcgttctg catctgaaaa tcgccaacaa
     6841 agcggcggat tccaaagttc tggtcaatca gctggcggat ttggcggaaa caataactct
     6901 aatcaaacat ctcaatcatc caacggtatg cctgatttcg accgtgatac ttctgaccca
     6961 ttcggctcaa gttcaacgat cgacatttca gacgatgatt taccattcta aacgaatttg
     7021 ataggaggga aatagaatgg cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga
     7081 ctacattgct gctaaccata ttgaatatat cgattataaa gatatcgaat tactaaaaag
     7141 atttatttca gaacgcggta aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa
     7201 ccaacgtaaa attactgttg caatcaaacg cgctcgtatc atgggcttgc tacctttcgt
     7261 tagcgaagac taataaatcc taggaattga atgactgatc cttttgatca gctattcaat
     7321 tccttttttt ctgtaaaaat aacctctatt cgtttagtcg ctcgaatgac gaaagttatg
     7381 gtaaaatcaa attagtcata tatgggagga aaattatgga aaataaaaac aatcatttat
     7441 ccccgaaacc tctcttcctt atcctgttta tattaatcga agtattggta gtcttaattt
     7501 taccatttcg ttttgttagc gcttcggtca ttctattgat gaatttatgg ggtgttcgct
     7561 attggttgaa aatgaaacgt tggcttattt taaataaacg tgaaaaaatc cagcaggctt
     7621 cgactgtagc tgaaaccaat ctagcttatg tctcagaagt catgcctgta ggagtgattg
     7681 cttatcagcc atcaagccaa aaaatcgaat ggctcaatcc attagcattg aaaatcaaag
     7741 atcaaagtgg attaacagag gaagaattgc tagatacttt ttttactgca aaagaaaaaa
     7801 agaaaaacca agtctatcta gcagataaaa catttcagtt tgatatggat acagtaaagg
     7861 gtgttgttta ttttaatgat attacccaaa accagttgtt aaaactccat cagacgaaca
     7921 gtcagccggt gattggtatt ttatctattg ataactatgc agatgccatt gataaattag
     7981 atgacaaaga gatttcttat ttgaactctt tcgtcactac actagtttct gactggatga
     8041 atgaatacca tgtctttttc aaaagaatca acgcagaacg gtttttcttc gtagcacaga
     8101 tgatcgatct gaaaaagatg atggataaaa aatttgattt gctggatcgc tttcgagcag
     8161 aagctgaaaa acagagctcg cctatcaccc ttagtatggg gattgcttat ggctcaagaa
     8221 ccctcgatga aatcggtgga gaagctcaga ataatttaga tgttgcttta gtccgtggag
     8281 gcgaccaagt tgtcgtgaaa gaggtaaaag aaggagcaaa acctgtttat tatgggggga
     8341 aatctgctaa gaatgtcaaa cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc
     8401 gtcggatttt tgatgaaaca ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg
     8461 atgctttagg ctctgctttt ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt
     8521 acatcgttat caatgagaac gagatcattc cagatgtaga acggtgtctt gaagaaatcc
     8581 ataagcatcc tgaattagaa atgcaactaa tctctccaga acgtgccata gagctgaaaa
     8641 aagaagagga cctcctcgtg atggtggatt accataaacc ttctctatcg atctctcagg
     8701 gcctatatga gcaatttgat aagattgtga tcattgatca ccatcgtcgt ggggaagagt
     8761 ttccaagtaa acctctgctg acttatattg aatcttcagc ttcttcagct tcagagctag
     8821 tttctgagct gatccaatac caaagcagca aaacaaatcg cttagatcgt tttgaggcca
     8881 cattattgct tgcagggatg gttgtagata caaatagttt caatgtccgt acaactgcac
     8941 gaacttttga cgttgccagc tatttaaggt cttgtggagc agacgcatcc ttagtacaat
     9001 atttattgag ctcggattta gcgacatact tggagatcag caatttgatt gctggaagcg
     9061 actatgtgac ttctgatatt gttgtggcgg cagggacaga cgaaaaagag tataatagtg
     9121 ttatagcagc aaaaacagca gatacattgc tttcgatgat caatatcaat gcagctttcg
     9181 tcattacaaa gcggacagac ggcttgattg ggatcagtgc cagaagtacc ggcacgatca
     9241 acgtccaaat catcatggaa tccttaggtg gcggaggaca cttcacaaac gcagctactc
     9301 aactggaaaa tacgacagtt gaggaaacaa aacagaagct atttgctgca atcaaagaaa
     9361 atatgaatca aatatatgat aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt
     9421 aaaaggaaaa ggtaaaaaag gggacatcaa agaagttcca gcaggttatg cacaaaatta
     9481 tctaatcaaa aataacttgg caaaagaagc aacgaaaagc agtatcagtc agttagcagg
     9541 acaaaagaaa gcacaagaaa aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa
     9601 agaattcttg gaaaaagaag atactgtagt cgaattgaag gcgaaagcag gggaagacag
     9661 ccgtttgttt ggttcgatcc cttcaaaaca aattgctgaa gggttaaaca aacaatacaa
     9721 aatcaaatta gataaacgaa aaatcgaatt agaaaatccg atccgttcac taggctacac
     9781 aaacgtacct gttaagcttc atcatgaggt gacggctacg atcaaagttc atgtcgtaca
     9841 agaataacaa atcttgatag cgatgttcag ctggtaagta taaccagaag gaaaaaagac
     9901 aaaccgaaat ttatctgaga aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt
     9961 tcagattgat ttcggtttct tttttgggaa cagcaaaata gcataaagaa aacgagtata
    10021 ataaaagaga actatttttt tattacactt tcggtacgaa tgatgacttt tctctcattt
    10081 tcttgtaaaa tgaagcaatt gaagacaagg agcgatacgt atgaatgaag tgtggcagga
    10141 tcgaatccca ccgcaaaata ttgaagctga acaagcggtt ttaggctctg tttttcttga
    10201 cgcagaaacg atcattgatg ccatggagta catagagcca aaagactttt atcgtcgtgg
    10261 ccatcaaatt atttttgaga caatgatcga gttaaatgat cgtaatgaag cgattgacgt
    10321 ggttactgtc aaagaccggc tggaacaagc caatttgtta gaagatgccg gtggtttaag
    10381 ttatctctca gacttggcat tagcagttcc gacagcagca aatatcgtct actatgcgaa
    10441 aatcgttgaa gaaaaatcat tgctgcgtac attgatccag acagcaacag gaatcgttac
    10501 gaaaggattc gagcaaggag aagaagttca aacaatttta gatgatgccg aacgcagtat
    10561 cttggaggtt tcggaaaaaa gaaatcgaag cggcttttta tctattgctg aagtactgaa
    10621 ctccacgatt gaaaatatcg atcgactagc tcaaaacaat gaagaaatca caggtctacc
    10681 aactggctat caaggtctgg ataaaatgac agcaggtttc cagcccgaag aactaatcat
    10741 tcttgctgca cgtccagcgg taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt
    10801 aggaacaaaa acagatcgtt ctgttgcgat atttagtttg gaaatggggg cagaatctct
    10861 tgtaaatcgg atgctttgtg cagaaggttc gattgaggcc agccatttac gtacaggtca
    10921 gctttcggaa gaagaatggt cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa
    10981 tatttttatc gatgatacac cagggatcaa aatctctgaa atccgtgcga aatgtcggaa
    11041 actagcgcaa gaaaaaggca atctaggctt gatattgatc gattatttgc agttgattga
    11101 aggtaacggt aaagagaacc gtcaacaaga agtatcagat atctctcgcc aactgaaaaa
    11161 actggcgaaa gagctgaaag tacctgtcat tgctctttca cagttatctc gtggagtaga
    11221 acagcgacaa gacaaacgac cagtgctaag tgatattcgt gaatccggat caattgaaca
    11281 agatgcggac atcgtagcct tcttgtatcg agatgattat tatgaaagag aaggcggaga
    11341 tgacgatgat ggaaacgagc caaaaaatga caacgtcatc gaagtcatca tcgagaagaa
    11401 ccgaagcggt gcacgaggaa cagttgaact actgtttatc aaagaataca ataagttttc
    11461 gtcgctttcg ccaagagaag aattttaaag aagaggttgt gagccttgag caatcccgag
    11521 agctctcgta agaagatgca aggagtaacc gcaggaacga ctagagtact taatttaaac
    11581 agatctccaa agggattgtg acaaaagtct tgtcacagtc cctttttccg aataaacggt
    11641 gttcaatcag ctgacccttg gtattagctc agcaaacgtc aaaatatgag gaactatttt
    11701 gacgtttgtt tctctaatac tcaggtcaaa acgctgatct tacgacctct tttccttaaa
    11761 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tcatcaaaca tgagaagcag
    11821 ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctttctta
    11881 aacggtgttc aaaagcttgc atctgcggta atacgcccaa tcaaacgaaa aatatgagaa
    11941 gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca caacctctga
    12001 ttcacggtgt tccaaaagta gcttcttcga aaaataagtc gaaatttcac aaaaatttga
    12061 gaagcaattt tcgtacattc cttcttattt cttgaagctg accacttctg ttacaacctc
    12121 ttttttaata accattctaa aaagataaag ttcgtttttt aagcgttttt ataaaataaa
    12181 aataaaaatc attcgatttt atttgaaata tgtatctatt attgatacaa tggttcagga
    12241 ttaaataata tagaacgagg tgttcacatg tcatctgtag tagtagtagg tacgcaatgg
    12301 ggcgatgaag ggaaaggaaa aatcacagac tttctcagtg aaaatgctga agtgattgca
    12361 cggtaccaag gtggcgacaa tgcagggcac acaattaagt ttaacggtgt gacttataaa
    12421 ctgcatttga ttccttctgg gattttttat aaagacaaga tcagtgtgat cggaaacggc
    12481 gttgtagtca atcctaaatc attggttaaa gaattagctt atttacatga aaaaggtgta
    12541 gaaacaagta atttgcgtat ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa
    12601 ttggaccaat tacaagaaga tgctaagggc gaaaataaga ttggtacaac gattaaagga
    12661 atcggacctg cctatatgga taaagcagct cgtgtaggta tccgtattgc agacttatta
    12721 gataaagaaa tttttgaaga acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt
    12781 gtaaaaatgt ttgattcaga accacttgat tttgacgata tttttgaaga atattatgaa
    12841 tacggacaac aaatcaaaca atacgtcaca gatacttccg ttatcttgaa tgatgctttg
    12901 gatgcaggca aacgcgtgtt gtttgaaggc gcacaaggtg taatgctgga tatcgaccag
    12961 ggaacttatc cttttgttac ttcttctaac ccagtagccg gtggggtaac aatcggaagc
    13021 ggtgtagggc cttcaaaaat caataaagtt gtcggcgtct gcaaagccta tacgtcacgt
    13081 gtcggtgatg gaccatttcc aactgaatta tttgatgaaa caggagaaca aatccgcgaa
    13141 gtaggacgag aatacggtac gacaactggt cgtccgcgtc gtgtaggctg gtttgattct
    13201 gtagttatgc gccattctaa acgagtttca ggtattacca acctatcttt aaactcgatc
    13261 gatgtattaa gtggattgga aacagtaaaa atttgtacgg cttatgaatt agatggcgaa
    13321 ctaatctacc actatccagc aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa
    13381 gaattgccag gctggtcaga agacattaca ggatgcaaaa cacttgcaga actgccagaa
    13441 aacgcacgta actatgtacg tcgtatctca gagcttgttg gtgtacgtat ctcaacattt
    13501 tctgtcggac ctgatagaac acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa
    13561 ggataaaaac gacgactgaa tcaggacgtt cttaccagag cgagtcgaag tgtttatcta
    13621 tacgataata ataacttgaa gaggtagtga aagagacgtt tagccttatt gccgataggc
    13681 tgtctcttga acactgttta ttcagaataa gggactgtga caaaaaccgt gtcacagtcc
    13741 ctttttccga acaaacggtg ttcaaaagct tgcatctacg gcactaagcc caatcagatg
    13801 aaaaatatgg agtgctattt ttatctgatt gttcttactg cttgatgcag agcagctttt
    13861 tcacaacctc tccactaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    13921 tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg tgactcggag ctaaacggca
    13981 ggctcacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    14041 acggcaaaat atgaggaact attttttccg tttttttctc taatactcag gtcaaaacgc
    14101 tgatttcaca acctctgatt cacggtgttc cagaagtagc ttctacggaa ataagccgaa
    14161 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    14221 acttctgtca caacctctta ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg
    14281 tctagcgcaa gagatttgct ataatagacg ataagggtaa aatgtaacga gaaggggttg
    14341 aaaaatgtgt atcaaattgt aacagattct tgttgtgatc tgccatacca tgtattagca
    14401 aaagaaaaag ttgatttttt atcaatgaat ctccaattga atgatcaaga gttagtagat
    14461 gacctcggaa agacattcaa ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg
    14521 cctacgacat cacaaatcaa tgttggccga tatttagaat ttttccgttc ttatgcggaa
    14581 aaacagattc ctgttttata tgtggctttt tccagtggat taagcggctc ttacagtagt
    14641 gcgcttcaag cggttgaatt gctgaaggaa gaatatgaag accctgagat ctatgttttt
    14701 gatacaaaag ctgcaagtat tggggaaggg cttttagtat tggaagctgt aagattgaaa
    14761 aaagaaggta aaagcatcga agaaacccta gagtggctgg aaagtaatta tttgaaagtc
    14821 cattcatggg taacagtaga cgatctgaaa tatctagaac gaggcggtag gatttccaaa
    14881 gcaagtgcta ctcttggcag tctattgagt gtaaagccta ttattacggt ggataaagaa
    14941 ggaaggctga cgaatgttga caaagttcga ggaagaaaca agtcgatcca gacaatcgtc
    15001 aatgagacgg taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct
    15061 ggggatctag agagcgcgga gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa
    15121 gatgtgaagc tttatccctt aggaccaacg atcgccagtc acacaggcta tggctgtatc
    15181 gcgatttttt cgatgggaat cagtcgataa aagcagtaac tcattctgta aaaaagccca
    15241 gaaattgatt gtaacaaaaa cagcgactct taaaagatag atgagcatcc tatctcttag
    15301 aggtcgctgt ttttttattt tttaagctaa ataggccatt atgcccataa ctcgttcaat
    15361 tgtttttgaa tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc
    15421 acacctttat ccgaaacggc aatgatgcgg tttgccaatg tttggataaa ttgatggtca
    15481 tgtgacgcaa ataagattga gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt
    15541 gattctaagt ctaaatggtt cgttggatca tctaatacaa gtacgttagc tttcgaaagc
    15601 atcatttttg aaagcatgca acggactttc tctccaccag ataaaacatt cactggtttc
    15661 aatacttctt caccagaaaa caacattctt cctaagaagc ttcttaagaa ggtgttatca
    15721 tcttcttctt tacttgcata ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat
    15781 tctttcgttg tatcctttgg taaataagaa ttacttgttg tcacacccca acgtactgtt
    15841 ccagtatctg gcgtaatttc acccatgata actttgaata gcgttgttgt tgttatatca
    15901 ttttctgcga cgaaagcgac cttgtcttct ttgctcaaat taaaagatat atcatctaaa
    15961 attttctttc catcaatcgt tacacttaca ttttctacct gcaataagtc gtttccaatc
    16021 tcacgttcag gtttgaatcc aacgaatgga taacgtctag aagaaggttg gatatcgtct
    16081 aaagtaattt tttcaagcat ttttttacgt gaagttgctt gttttgattt tgatgcattc
    16141 gcactaaagc gggcaataaa ttcttggagt tctttgattt gttcttcttt tttggcattt
    16201 gcgtttgctt gaagtttagc cgctagttga ctagattcca accaaaaatc ataatttcct
    16261 acataaagtt tgattttacc aaaatctaga tccgccatat gtgtacatac tttgttcaaa
    16321 aagtgacggt catgggatac aacgatcaca gtattttcaa aattgatcaa aaattcttcc
    16381 agccaattga tcgattggat atccagaccg tttgttggtt cgtctagcaa aagaacatca
    16441 ggtttaccaa atagtgtttg tgctaataac acttttacct tttgtccacc agttaattca
    16501 ctcatttgct gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa aacagctgct
    16561 tctggttcag cttcccatcc attaagctca gcaaattctc cttctaattc agctgctcga
    16621 ataccatctt cgtcagtgaa atcctctttc atgtaaatcg catctttttc tttcagcact
    16681 tcatacagac gcttgtgtcc catgatgact gtttcaagta cagtatagtc ttcatagtcg
    16741 taatggttct gtttcaatac ggccatccgt tcatctggtc ccatagtaac ggaacctgtg
    16801 gaaggctgca gatcccctga taacacttta aggaacgttg atttacctgc accatttgca
    16861 ccaatcaagc catagcagtt tccgggggta aatttaatat tcacatcatc aaatagtttg
    16921 cggtctgaaa agtgcaaact cacatcattt acagtaatca atttttttcc tcacttttat
    16981 cattattagt tttgacaagt cttatagaat tatatagggg aaaggtaaaa gattcaaggc
    17041 aatcgaacat tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga
    17101 gcgaaaatag ctactatagg atttattagt ttgcaacagc ataatttttg acgttattta
    17161 taagagggag tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa
    17221 aagaaccatc gactggggct cattgatttt gggaatttta tttgtacttg tctcattgtt
    17281 atcatttcaa gaccctgtcg gaaatttagt tgcaattgta gtagtctttg cgatttttgc
    17341 ctttattaaa gggatatttg aactgtttgt acggaacaga ttaaaagagc taacaggata
    17401 taaaggaaag atgcccttaa taatcgggat catcgatatt ctagttggcg tctttttctt
    17461 attcaatatt ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat ggtttattgc
    17521 tgattcagtt ttagcgttat ttactgctga cttggcaaga ggagtaagtg aaggatatta
    17581 ttggtttacg atcatcgtca atattttagg aattttatta ggaattttct tattgtttaa
    17641 tccaatttct tctgcgttga cattaagctt tttagttgga ttctatttta tgttatttgg
    17701 tatcacacat atcgtctatg catttagata agtaaaatca aacaaataaa aaagccggct
    17761 tccgattaca gttttactgt tgtaattgga agccggcttt tttatcatgg acaaatatgc
    17821 acgaggtgat ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag ctatatacac
    17881 aaattttatc ttaatactcg aagcatagcg cagttgtcac aacctctttt catataattc
    17941 attcaatact ggcaaatcat ggacttcata tttttctttg aatgcttgga tttcttcttc
    18001 agaatatttt cttggatcta aatgcagatc ttctactggc aatgggcgct tgttattgat
    18061 cttcacatcg attactacag gacgatctga ttttgcagct gcatcaaagg caaatcgaag
    18121 ttgcgagtat tctgtgactg taaagctatt tgccccgagc gcttctccaa ctttaccgaa
    18181 atctgcatct tctaaaaata caccaaattt cttttgttcc gtatcttctt gttctgcttc
    18241 gatgaaacca aaagaatcat tcgaaaagac aacattaata attggaagct gatatttgac
    18301 ttgtgtaatg atatcttgca tgaccatagc aaacccgcca tcaccactta aggtaaatac
    18361 ttgtttttct gggtagctta gttgtgcggc aattcctcct ggcacaccat tacccatagt
    18421 tgcgaaccat ccagatgtcg tatgtttttg tttaccgttc atattcaata aacgaataga
    18481 atggatagtc gtgttaccga catctgtgac gaaaatcgca tcgtcttcag caatacgatt
    18541 gatttcttta tagacagcct ctggacgaat cggttcttcc tctcgatctt caaaagattt
    18601 caaccaattt acccattttt ctttattttc ttgatttgct aggtaccagc tatcagcagg
    18661 gcgagcatcc cctaactcga caagtctttg caaagctgtg tttgcatcac cgaggatcga
    18721 caaagatgtt tcatggcgtc gtccaaattt tgacgcatcg atatcgattt ggataaattc
    18781 agcagttgga ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag
    18841 atcggcttct gccaatgctt catttccggg tttagtagcg acacgtccag caaaaccaag
    18901 aaagttttca taatcatcag gaataattcc ttttgctaga acggaagcga caacaggaac
    18961 tgaaaaatgt tctgagaatt ctttgatttg cggaaatccg tttcgcgttc cttgaccaac
    19021 ataaagaact ggtttttttg ctttttcaag ataaggcaaa gcagctagca aatctttttc
    19081 ttctggtaat atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat
    19141 ttcttcaaaa ccaagatcaa cagggatagt cacgatagcc acgcctttat gctcataggc
    19201 ggctttaatg gcttcatcta ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt
    19261 gtatacgcta acatctgcga aaataggatt ttcatttagt tcctggaaag agttatagtt
    19321 catggatgta gaagcaactt gtcctaagaa cgctactaga ggcacatggt ccatctgtgc
    19381 atcatacaaa ccattgatca aatgtgtcgc accaggtcct gcagagccga agacagcgcc
    19441 gactttcccc gttaattttg catctgcagc ggctgcgagt gcgccgactt cttcatgacg
    19501 aacttgaatg tagttgattt cggttttttc ttgatatagc gcatccataa ttgaattgaa
    19561 agatcctccg ggaatgccgt aaatatggtc gataccccag ctttcaagaa cttttaccat
    19621 tgcgattcct gcattgattt ttgacatggt gtttcactcc tttagcaatg atgatacata
    19681 aattttttgt atctagagta tagaactttt tatcgaaaat cccaactaat tcgtcttttc
    19741 acaaaaatgt agtcatgttt ttgggaatat gctagagtag tacatagata gattcaagag
    19801 aggggaatgg catggttgaa tggctattga gacttgaacc ttggcagcaa gcacttgctg
    19861 ggacagcttt tacttatttt atgacagctc ttggtgccgg gttggttttt ttctttaaag
    19921 aaatcaaaaa agaagtgcta aacttgatgt taggatttgc ttctggtgtc atgattgctg
    19981 caagtttttg gtctttgtta gatccggcta ttacaaaagc agaagaaaat ggagatattg
    20041 cttggctagt tgtaagtatt gggtttggtt taggcggtgt gtttttatat atggcagata
    20101 aaacactgcc ccatatgcat tttggaccac aacatgaagc agaaggattg cctacccatt
    20161 taaaacggac gatcttgctt gttttttcta ttacattgca taacattcct gaaggtttag
    20221 ccgtgggggt agcattcggt gcagcagcaa ctgcggataa tccgacagcc gctatattag
    20281 ccgctgtttc ggtggcgcta ggtattggta tccaaaactt cccagaagga gcagctgtat
    20341 caatcccgtt gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt
    20401 ctggaatcgt tgaaccaatt gctggagtga ttggagcgct actagtaacg aaagtggagc
    20461 tgctgcttcc ttatgcactt gcttttgccg caggagcaat gatttatgtc gtggtggaag
    20521 aattgatccc tgaagcacag cagacattgt ccagcaaacg tcattttgca gtttttggcg
    20581 tcatgagtgg atttatcatt atgatgattt tggatgtggc attaggataa ataaaaaaaa
    20641 tatatatgat atatcaaata gggccgcgaa cttatatcgc gaccctattt ttttagtaag
    20701 ttatatcata atgaagtgaa tggtatcata cattaataat agttttttct atgtgtattt
    20761 cctttgatcc agcgaacaag ccagatgata agtgcgattg ggattagaac tttgaccgca
    20821 aaccataaaa gcccgcttag gacactgaca aaaagactga tgacgaagct agctgcaatt
    20881 agcactaaaa gaaccatgaa gatatttaag aacatatttt tttgacgcat acaaaaccct
    20941 cctcttgttc attctttact atagaaaaag tataacaggg attgtttaaa aacacctcca
    21001 actgaagact gatttttact tcatctactt aaatgatcaa acaattgctt tttctctggt
    21061 ttatcatcta aaatagagcg gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag
    21121 catttacaga tgctgtaatc gcgattatca tgactatttt agttttagaa cttcatgaac
    21181 ctaacggaga cacgtgggag gcgctcgctg cattaggtta ccgatttttt atctatattg
    21241 tcagttttat cattttagcg atttattgga acaatcatca tcatttattt caagttgtac
    21301 ataagataaa tggacgggtc ttatgggcga acagtttctt tgtttttgcg ctttcgcttt
    21361 ttccgtttgc aacttcatgg atcagtgagt acatcaatag cctggctcca gaaatgactt
    21421 atggttttgt taccttgacc gctaatattg cgtattatat cttggcaaaa gaacttgtca
    21481 aagcaaataa aggccacgag aaagcattga aggcgatgtt gaataattac aagaaatcgt
    21541 atctatcgat tggtttgaat gtgttaggaa ttgtcttagg gtatttagta gcgccagtag
    21601 ccgtgctgat cgcaaacatc gtgattctag ctgcttggat catcccaagt cgtaccatcg
    21661 aatcacaata tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat
    21721 aaataaacga tcaaatatcc gagtgatact agtattcaaa gagggatgca gatggatcaa
    21781 aaaagaattc atgtaggact gattactagt tttgtcacga tgcttattta tttggatcaa
    21841 ggtcatttga cttggacagt agttggtgtc gtaggatttt tgacattatt cacagcaata
    21901 aaaaaataaa catctttatg taatctgatg ttctatctat ttaagaaaag catgtttaca
    21961 gcagtatgat tctgttgtaa acgtgttttt atttgtggaa aatttaattt ttagaaaagt
    22021 gtatcgaaaa atagatagtt agtttttcca cagtggataa cttcgtaaga gataaaaata
    22081 aaaaattagt caaagaaggg cgtatagatt tc
//
LOCUS       DAJSVP010000036        22070 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSVP010000036 DAJSVP010000000
VERSION     DAJSVP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.036"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            94..3372
                     /locus_tag="ON449_001427"
     CDS             94..3372
                     /locus_tag="ON449_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3934020.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3913..5820)
                     /locus_tag="ON449_001428"
     CDS             complement(3913..5820)
                     /locus_tag="ON449_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3934021.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5950..6825
                     /locus_tag="ON449_001429"
     CDS             5950..6825
                     /locus_tag="ON449_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3934022.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6905..7879
                     /locus_tag="ON449_001430"
     CDS             6905..7879
                     /locus_tag="ON449_001430"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3934023.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8059..9204
                     /locus_tag="ON449_001431"
     CDS             8059..9204
                     /locus_tag="ON449_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3934024.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9341..9685
                     /locus_tag="ON449_001432"
     CDS             9341..9685
                     /locus_tag="ON449_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3934025.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9956..11242
                     /gene="efmA"
                     /locus_tag="ON449_001433"
     CDS             9956..11242
                     /gene="efmA"
                     /locus_tag="ON449_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3934026.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11380..11979)
                     /locus_tag="ON449_001434"
     CDS             complement(11380..11979)
                     /locus_tag="ON449_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3934027.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12383..13504
                     /gene="mnmA"
                     /locus_tag="ON449_001435"
     CDS             12383..13504
                     /gene="mnmA"
                     /locus_tag="ON449_001435"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3934028.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13750..15171
                     /gene="arcD"
                     /locus_tag="ON449_001436"
     CDS             13750..15171
                     /gene="arcD"
                     /locus_tag="ON449_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3934029.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15185..16303
                     /locus_tag="ON449_001437"
     CDS             15185..16303
                     /locus_tag="ON449_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3934030.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16363..17655)
                     /locus_tag="ON449_001438"
     CDS             complement(16363..17655)
                     /locus_tag="ON449_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3934031.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17787..18365
                     /locus_tag="ON449_001439"
     CDS             17787..18365
                     /locus_tag="ON449_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3934032.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18494..18871
                     /locus_tag="ON449_001440"
     CDS             18494..18871
                     /locus_tag="ON449_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3934033.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18804..20876
                     /locus_tag="ON449_001441"
     CDS             18804..20876
                     /locus_tag="ON449_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3934034.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21087..22034)
                     /locus_tag="ON449_001442"
     CDS             complement(21087..22034)
                     /locus_tag="ON449_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3934035.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7135 a         4038 c         4684 g         6213 t
ORIGIN      
        1 cacttattat aattttgttt tttattgaca gtaaaaataa attaggtaat actttaagta
       61 aacgctttct aatttgttac gggaggaata cacttgttaa aaaaacgtat aaaatacagt
      121 gcgctaatga tgaatctcct tatgctcagt accccggttt taatttctgt cgttgaagtt
      181 gctgctaacg aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat tgaagggatg
      241 tcaacaatgg aggaaatacc aagaactagt gaagatatgg atacggaaga aggaaatgat
      301 cctatgatga aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa
      361 gagtggaaag gggttgtttt cggagaaagc acaagtgcta gtaggtatgc cattgaacca
      421 ttagaagacg gcgtgcggct ctcttccacg aataacggcg gaaaattcca gtcaagcgga
      481 tcagatggaa tgacctatta ttatcaagca atccccaaag acatcaactt taggatgcgg
      541 gcgacgatcg aaatagaatc ttggacctat acgaatgccc aagaaggatt tgctttaatg
      601 gtaagagacg cagtaccaaa aaatcatttg tttgggcagg cattttattc aaattcattt
      661 gccatactag gcagtcggat cgaatatgtc tgggactcgg atagacaaga agtggtcaac
      721 acgagcggca gcaaatatac gatgcgactt ggattgggga cacgggcaat cacaggaata
      781 tcatctgaag atccgcaagc tgcgccagct cctgggagtg tctcagtgga aacaacacct
      841 ttagagactt cagcagggtt cttggaaaaa gaaacaggtt cttataatat attcggaaac
      901 ggtcatggga atctgtttcc ggccacgaac tcgattacta aactggacgt agagatgaaa
      961 aaaacgaata ctggtttaga ggcatattat tacgatcctg aaacaggaga agaaatggct
     1021 tctgacatca tgtatgactg ggaaaaatta tacgcttcag atgaatcggt tatttatgca
     1081 ggatttgctg cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt tgcttactca
     1141 gatcctgcga cagatcctcc tgggcaagaa agacctactc gcttgattga tccgatttac
     1201 actgtgacct ctagtaatca ttcaggaaac caagatcata ctatgtcctt tagagcaaat
     1261 gctgacggat tattgacaat taaaaataaa gcaacaggag aggtgcttaa gaacgcaaaa
     1321 aacttggcta tcactgcgaa tcgggaattt gattatcaga caaaactgat ggaaggaacg
     1381 aacgaattta ctttcagttt tacgccagat aaaaactatc cgccagaaga atacgtagag
     1441 atgactagtt atgaaacaaa agagattctt catacagtgg actaccgtaa acctaaatat
     1501 tcgacagttt atgtaacacc agaagggtca gatgcaggaa acggaagcag acaaaatcca
     1561 ttatctctga cacaagcatt gagatacgca tatgctgggc agacaatcgt catggcgcct
     1621 ggaacttatt ccttcgaaag aggattgacg attccaaaag gcgttaacgg gacaagagaa
     1681 aacccgattc agttgattgc agagaaaaat ccagaaaaca agcgtcctgt acttgatttt
     1741 aaaggaactg gaaatgggat gacactgttc agtcactact ggggtttacg agggtttgac
     1801 attacaaata gtgcagctat gcaaaaagga ttacagattt ctggaaatca taatttgatc
     1861 gaagagattc atgcttatcg gaatggaaat acaggaatcc aaatcagcgg tagctcaaat
     1921 gatcctttcg aagcatggcc cgcgcataac ttagtcaaga actgtacatc ttttgaaaat
     1981 gccgatccag gatttgaaga tgccgatgga tttgcggcca aactgacaat aggagaagga
     2041 aatgtatttg acggttgtat cgcgtatcat aatgcagatg acggttggga cctttttgct
     2101 aaaaatgaaa caggttcgat cggaaaagta atcatcaaaa actcagttgc ttatcgaaac
     2161 ggctgggtac caggaattga gggagaaggt aacggtaatg gattcaaaat gggcggttct
     2221 tctttaacgg gccctcatga attgatcaat agtttgtctt tcgaaaatct tgcgaaagga
     2281 atcgattcca atagcggaac agatattatc gtcaatagca gtacaagttt taataacggt
     2341 tcatataatg tggctcttta tacctcttca gcaggaaaca cagactacca tgcttctggt
     2401 atcttatctt tccgaacaga gaatcttgag atgagagagc agatccgccc attgaatcaa
     2461 aacgaagatc aaatctacca ctcgttgaac tactattgga atgaacgaga gaaacaatca
     2521 gaaaatactc ttggacatac catatcaaaa gattggatcg agtctctaaa tacatgttcg
     2581 aaagaaggtc agcagcctgt tacgcgctat gaaaatggaa gtatccatgt tcatggactg
     2641 atgcagatta aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg tcttccatta
     2701 tctgtaggtg ctatttttga cggagaaacc tcaccgtctt cggaatatcc agagtatgca
     2761 atcgtacaag aaccaaatca gacaaaacca aatgaacaaa ataatccgat tattatacaa
     2821 gaagcaaatg gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa gccttttaca
     2881 gtttttgtgt caactttcac accaaatcaa ggaacgcctg tctttgaaga tatgcgttat
     2941 ttcccagaaa agatccaaat cgttgaaaca cagtcaagtg ctcaattttc taatcatacc
     3001 gctatttttt cagcaccttc aaaaggagaa tatacgctag aagtcactta tcgttcggaa
     3061 atatttgatg gggaaaaatg gtctgctcat acagaatctg ttacccagca aaaagcgatc
     3121 actgtgcgag aaactgccga tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca
     3181 ggaaatgaat caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga taggacagca
     3241 aaagaaagtg aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt taataacccg
     3301 ttgctagcat tagctggagg aattttgtta atcggtgtag ttgtttatgt catgaaacag
     3361 cgaaaaaaat aagtggaatt tctcctttta tgatgtaaaa caattgactg aggaaagtaa
     3421 aaatagaagg aaatgagacc aatagatgtc tcacttcctt ctatcttttt atagatgagg
     3481 ttctccttgc tgcttttgat tggctgttct tattacttga tgcaggacag ctttttcaca
     3541 atctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacggcaa
     3601 aatatgagga actatttttc cgtttttttc tctaatactc aggtcaggac gctgatttca
     3661 caacctctcg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca
     3721 aaaatgtgga aagccatttt cacaaaattt ttcttactac ttgaaactaa gcagcttttt
     3781 cacaacctct aatacatggt attccagaag taacttcttc gaaaaataag ccgaaatgtc
     3841 acaaaaattt gagaagcgat tttcccaaat tccttcttat ttcttgaagc tgaccacttc
     3901 tggcacaacc tcttaccaga ttcgcactct tttttctggc gctagataca tagcgtcttg
     3961 ttccgtgata ccaaatgttt catagaattc gtcaaggttt tttggttgca cgtttgctcg
     4021 gagtttagca ggggcatgga catcgatttg cagtaaaagt tgttggtatt ctttttttgc
     4081 ttttgtacgc caaatcgttg cccaattgac aaagaattcc tcgatagaaa gatctgattc
     4141 cgtttttgct gcttccaatg cacagcttag accgcctgca tcagcaatat tttccgaaac
     4201 ggttaatttt ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt cttcgatcat
     4261 tgcttgtgct ttgttttgaa attgtgtaag atcttcttct gtccaccagt tgttcaagtt
     4321 gccatattcg tcaaataaag cgccgttatt atcaaatgca tgggaaatct catgagcgat
     4381 aacagctccg attccaccat aattagcaga gcttgattgt tctagactgt aaaatggtgc
     4441 ttgaaggatc gctgccggaa agacgatcac attccgaaat ggatgataat aagcattgac
     4501 cgtgtcagca ctcatttccc attctgaacg atccactggt ttgttccatt tgctgaaacg
     4561 atcttttaat gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac caccctgatc
     4621 tgcaggtact gttttgaact ggtcgaataa tgctgggatc ttatcggggt agcctacttg
     4681 gatacctaag ttatttaatt ttatgacggc tttttctttt gttgcatccc caagccagtc
     4741 attttcttct agacgttttt tataaacggc gatcattttt ttcaccattt gttctacgtc
     4801 ttttttagca gcttcgccaa aatatttttt tccgtagtaa tcgccaacta cttgatcgaa
     4861 ttgtccagag gcaaggtaat acgctgcttt tttctgaggc attgcttcgt ctgttccaga
     4921 caatacgcga gaatagattc cgctgacttg gcggaattct tctgatagat aaccactaag
     4981 agaacgtact gttttaatga tcatccagct tttgaataac ggaaaatgag cttctgtcag
     5041 gatgtcattc aaatgatcga agtacactgg atcagttacg atcactttat ctggagttgt
     5101 cccaatcaaa cccgtgacaa gttttttcaa gtccagttta tctgtgtatt taataaatgt
     5161 atcaaaagat tgcggattat acattttgct gtaatctgcg ttttcttctg cactttttac
     5221 atgaggagca atcaatttat caaattgcac tgcttgttcc gtgattgctt ctgcctgact
     5281 tttttctttg ccggcaagct ctaataactg aacagccata tcaaagaaaa cgcctattag
     5341 ttgtgcaccg ttgggatggt ctgatgcata atacgtctta tctggaagga ataaagaagg
     5401 cggataagca aagaaggcgt tcgtttgcgc attcttcata tctgcatcta catccagatt
     5461 aaatggcaat ggtaatgtat ccaacagcca ttctggaaat tgcttattca attcgtcgaa
     5521 agactgcaat gattcaactt tttcgattat aggttgaagc ggttgagcat gcaactcatt
     5581 tcttcggtca taatcgtttg ccagttgata atatgcaagg aaatgtttca ttttttctga
     5641 agacactttt tctggatggg caagcagatc gtctgtttct cccatcaaaa gttggtcgat
     5701 cccgtcaact aaatcttgga aaccgcctgt agctggttta tctgcaggga tttttgctgt
     5761 tttttgccat tcttcattga ctgcttcata gaaatcttgt ttcaatcgtt ctttgttcat
     5821 tcatcgatcg ctcctttttt gtttcgtgtg cccattatct tacaaaatga aggaattaac
     5881 aaatagaaca taaaaaattg aaaattcgaa caatcaaact gatttgtttt tttcaatcct
     5941 tcacttataa tgaaaaaaag gaggcgatcc caactgaatc aagtagtgga taaaccttta
     6001 tatttcaaag tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga
     6061 aaaagtaaat tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat
     6121 caatcaggac atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct
     6181 tgtaaaaaat ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga
     6241 aaagaagata ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc
     6301 tgggtgggat tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc
     6361 tatctttgtg gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag
     6421 gggtggatgc ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg
     6481 aaggcattgc gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt
     6541 acagggattt ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt
     6601 ctaggttcat ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt
     6661 attttgaaag tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt
     6721 gaaactgaaa caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa
     6781 gtcattcctg ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt
     6841 aacagaaaat cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga
     6901 ctacatgagt aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg
     6961 tccattagct gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa
     7021 gcaatttagt gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt
     7081 gtatatcgtt caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat
     7141 gatcgatgca atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg
     7201 gtatgctagg caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc
     7261 taacttgatc gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca
     7321 agtacaagga ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca
     7381 ttattaccgt caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag
     7441 cggtgttcag cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga
     7501 tcatgcagat gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac
     7561 atgtatcatg gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt
     7621 attaaaagaa aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga
     7681 tcatgcgaaa agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt
     7741 tttcacagaa gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat
     7801 gggtgaagcg gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga
     7861 agaaaaagaa ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag
     7921 cttcaaggag ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt
     7981 cctctagaca aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct
     8041 agcaataatt tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact
     8101 acccccatgc atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga
     8161 aatccttcaa gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga
     8221 caaatcattg cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga
     8281 acagaagggg ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga
     8341 catctaatca ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag
     8401 acaaaggggt ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa
     8461 gaggtcaaaa aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat
     8521 gaaatcggaa atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct
     8581 tatttccata cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta
     8641 ggaatcgatt tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc
     8701 ttatataaac gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa
     8761 aagcggcgtg caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa
     8821 ctgctgtcac ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg
     8881 attcttgata cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat
     8941 aaactccctc atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg
     9001 aagcttgatc ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc
     9061 gagccttcac acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc
     9121 atccgtatca gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa
     9181 ttagttcgtg ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag
     9241 agaacgcctt cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact
     9301 tttataataa agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc
     9361 aacggtactg ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt
     9421 gcgcgataat ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga
     9481 tgccacgccg caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca
     9541 aacattgaaa atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga
     9601 tccaaaacag aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg
     9661 tgggttattc gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg
     9721 gcctattgtt agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt
     9781 taaatttaac acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga
     9841 aatttaaatt gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg
     9901 tataatgatt gttgtgcaaa cgctatacaa aatgaaaaag aaaagaggga tgtttatgga
     9961 aaatgaacag tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg
    10021 acagtttttg tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac
    10081 aagagaaacc ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat
    10141 ggttttacta agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt
    10201 gattgtcacg gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat
    10261 ttcagaatcc ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca
    10321 aacattccaa atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat
    10381 aacaaggacg aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc
    10441 tctaggtgcg gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt
    10501 gggggcagtt tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga
    10561 aatattggaa gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat
    10621 ggataataaa gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat
    10681 gccagcaatc agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca
    10741 ggcgggagca gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag
    10801 ttttatagga acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg
    10861 cctgacgatc ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct
    10921 tatcttgaat gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat
    10981 gatccagcag agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt
    11041 gatgaacctg gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg
    11101 ggtagaaaaa atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt
    11161 tttaacgtca gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca
    11221 ccatgagcaa aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc
    11281 catttctgat cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat
    11341 tatctttatt tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct
    11401 ctttgcttca tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt
    11461 ttcctttaag agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg
    11521 tacgctgtaa tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa
    11581 catcggataa tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc
    11641 ataaaaataa aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg
    11701 gaaaaaagcg cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt
    11761 gaccagttcc aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc
    11821 tagatgataa acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg
    11881 gttcatttca tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg
    11941 ttcatggaaa aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt
    12001 tcttagagta aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg
    12061 taaatgagac gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat
    12121 ttataaaatt attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt
    12181 atatcgaatg ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat
    12241 aggtccagta gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata
    12301 tgacgtataa tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc
    12361 taattgaaat gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat
    12421 gagcggcggt gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt
    12481 gatcggaata ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac
    12541 ggaagattat aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt
    12601 gaattttgaa aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc
    12661 aggacgtaca cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt
    12721 ggattatgca atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac
    12781 tcgcgatgaa aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac
    12841 ttacttcctt agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg
    12901 catggaaaaa tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa
    12961 aaaagattca acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa
    13021 ctatctgcca gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca
    13081 tgctggcttg atgtattata cgattggtca acgtcaagga ctagggatcg gtggcggagg
    13141 agattcgcaa gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt
    13201 ggggcaaggg ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca
    13261 ctttacgacg aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg
    13321 tcagcaagac gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt
    13381 cgacgaacct gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga
    13441 atgtctgggt ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt
    13501 ttaagcgata ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt
    13561 ggatagcata gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata
    13621 cttgtgtaaa aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag
    13681 agcagttgca aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg
    13741 gtgatggaaa tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt
    13801 gtcagttcgt caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca
    13861 gctccagggc cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta
    13921 ttatcgctga atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac
    13981 gcggaagctt catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct
    14041 gcgtggctgg gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc
    14101 ccgatattca aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg
    14161 ggtctgactt tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg
    14221 acgatctgta aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa
    14281 gtgaatatat tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca
    14341 gggcagcaag gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc
    14401 tttgtaggga tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc
    14461 cagcaagcat cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc
    14521 ttgccatacg gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca
    14581 aatattttaa aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt
    14641 atttcgatca ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg
    14701 atggctcgag acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca
    14761 acttattcgt tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt
    14821 actgattatg cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat
    14881 ctacttgtcg ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta
    14941 gtgatcggtg ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct
    15001 tatgtccttt tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt
    15061 cgtgaaacca aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg
    15121 gccgcactgc tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag
    15181 aaacatgaaa atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc
    15241 ggtatatgat attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt
    15301 agatgggact agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg
    15361 ctggatcgaa ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga
    15421 tccggaaaac atcttacaaa cgaatggagc gactggtgct aatcttttag ctctatacgc
    15481 gttagtggaa gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga
    15541 catccccaaa tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg
    15601 gcagtttgat ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt
    15661 gaatagtgca aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc
    15721 agaaattgct cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac
    15781 ggataaaggt gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc
    15841 cctatccaaa acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga
    15901 gttggcagaa gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc
    15961 agatgatctt gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa
    16021 gatcattaca gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga
    16081 acttgtcgct cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga
    16141 ccagacattt tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc
    16201 ctttgattta ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa
    16261 gggattattc ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg
    16321 acagtctgtg acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg
    16381 agagtgccct gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg
    16441 gatctcttgt gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga
    16501 aagaaagacg ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac
    16561 tacgttacca gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg
    16621 aggcgaatat tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc
    16681 aatcaaaaaa cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata
    16741 aaccagaatc gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc
    16801 taagcgacca aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat
    16861 tccgctgatc aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat
    16921 gattggacta atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat
    16981 gacgacgtgt ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt
    17041 tttcactggg acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa
    17101 gatgatccct gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc
    17161 aagaagcggt ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg
    17221 accacttttt tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc
    17281 agaaaaaatc ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc
    17341 catcaaagaa atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc
    17401 tttttggtcg gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga
    17461 tgaaaaagtc aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc
    17521 tatgtaaagt gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc
    17581 aaaccataag acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt
    17641 tattcttgca gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta
    17701 taaactacaa tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta
    17761 aactgaaaga gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc
    17821 tgtctcaaga gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc
    17881 aaataacctt tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc
    17941 atttcaaaaa tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta
    18001 atgaccagct gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg
    18061 cagaaactta tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg
    18121 taccgcatac caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt
    18181 tgcagatatt tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac
    18241 ttcgtagtat gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac
    18301 caacaatcga tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc
    18361 cttaaacaat cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata
    18421 agagaaaatt cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag
    18481 aaggagcacg agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt
    18541 ttttgaagac tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc
    18601 acgacaagcg tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc
    18661 aaaaagtaac tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg
    18721 tgacgatgat ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac
    18781 agtagaaagc agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg
    18841 cgaaagacct aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac
    18901 ccaaggtgat cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc
    18961 tgaataagga atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag
    19021 gaatcaaacc acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc
    19081 gaaaagatgt ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg
    19141 ctcgctggat cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt
    19201 ctcaaacaga taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg
    19261 atgcattgta tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg
    19321 tgaccagagc cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac
    19381 cgacactagc attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt
    19441 atttcacgat cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat
    19501 ttgctttaaa aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag
    19561 gaactgtcac agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt
    19621 taacagagat ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa
    19681 gtcttgtaca aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca
    19741 aatatctgac aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact
    19801 ttgctcctga ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat
    19861 tccaagacag taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt
    19921 atgaaaaatt aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag
    19981 gactgtttgc tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag
    20041 aacgattcat ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa
    20101 ctgagacgga aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca
    20161 aaaaagaact atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg
    20221 aaaaatacag tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat
    20281 ctgaactgat ggaacgtacc cctcaaggat tgcaagtatc tcctaaaggt aagcaattat
    20341 tagtattggt aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc
    20401 ttgaagctat tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag
    20461 aaacaaaacg cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga
    20521 ttacccaaaa gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg
    20581 gcaaaatcta tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag
    20641 tctctaatca tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg
    20701 gtgcttattt ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc
    20761 agaagaagaa aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag
    20821 aagaaccaga agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag
    20881 atatgaaaga ggttgtgata gaagtggtca gcttcaagaa ataagaagga atttacgaaa
    20941 attgcttctc aaatttttgt gaaatttcgg cttatttccg aagaagctac ttctggaaca
    21001 ccatttattc ggaaaaaagg actgtgacaa gacttttgtc acagtccttt ttcatttaaa
    21061 aaaatgttta cttcatttga cgtatattat tggtgaggct tcaattcatt tggcttcact
    21121 ttttctgttt ctcttatgat ataaacagga cgctgtttcg tttctaagaa gattttccca
    21181 atatatttcc cgatgattcc aagacataac aattggattc caccaatgaa caggaagatc
    21241 gagaccatgg aaggccatcc tgatgtagga tcaccaaaca acaatgcccg caaaataatg
    21301 aacaaaattg caataccaga accgacacaa gacaaagcgc cgataaatga agcaatcgtt
    21361 aacggtgcat ccgagaagtt cacgatacca tcaatcgagt agttgaataa tttccagaag
    21421 gaccaagatg tttcgccagc cacacgttcg cggttttcat aagaaatata ttctgtttta
    21481 aatccgaccc agctaaataa tccttttgaa aatcggttgt attctgttaa ttctaggacg
    21541 gcatccacca tttgacgagt catcaagcgg aaatcacgag cgccatcgac catttctgta
    21601 tcgctgattt tattgatcaa ctggtagaat tttttcgcaa agaaactgcg tatcgcaggc
    21661 tcgccttcac gagtcgaacg ccgggtgccg acacaatcga tatcagaagt agtgatccgg
    21721 ttgatcatct caggaagcag ttcgggcgga tcttgcagat caacatccat cacggtcact
    21781 aaatctcctg tagaagcttg caatccagcg tacagagccg cttctttccc aaaatttctg
    21841 gaaaatgaga caaagcggac ataaggataa gctgaggcta gcttcttgat tgctgctaac
    21901 gtaccgtctt ttgatccgtc gtcaataaac aaatattcga actcatgggc acctttgaat
    21961 ttttcaacct catcaaaaaa aagaggaaca gttttttctt cattaaaaca tgggacaaca
    22021 attgatactt tcatcgtttc tatcctttca atcgtatgtt tatatatggt
//
LOCUS       DAJSVP010000037        21956 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSVP010000037 DAJSVP010000000
VERSION     DAJSVP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.037"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            28..1626
                     /locus_tag="ON449_001443"
     CDS             28..1626
                     /locus_tag="ON449_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934036.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ON449_001444"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ON449_001444"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3934037.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="ON449_001445"
     CDS             complement(2702..3337)
                     /locus_tag="ON449_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3934038.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="ON449_001446"
     CDS             complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="ON449_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3934039.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4923..5921
                     /locus_tag="ON449_001447"
     CDS             4923..5921
                     /locus_tag="ON449_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934040.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5934..7952
                     /locus_tag="ON449_001448"
     CDS             5934..7952
                     /locus_tag="ON449_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3934041.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8728)
                     /locus_tag="ON449_001449"
     CDS             complement(8012..8728)
                     /locus_tag="ON449_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3934042.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8955..9497)
                     /locus_tag="ON449_001450"
     CDS             complement(8955..9497)
                     /locus_tag="ON449_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3934043.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9502..9729)
                     /locus_tag="ON449_001451"
     CDS             complement(9502..9729)
                     /locus_tag="ON449_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3934044.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9808..11688)
                     /locus_tag="ON449_001452"
     CDS             complement(9808..11688)
                     /locus_tag="ON449_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3934045.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11877..12491)
                     /locus_tag="ON449_001453"
     CDS             complement(11877..12491)
                     /locus_tag="ON449_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934046.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12633..13193
                     /locus_tag="ON449_001454"
     CDS             12633..13193
                     /locus_tag="ON449_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3934047.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13498..13812)
                     /locus_tag="ON449_001455"
                     /pseudo
     CDS             complement(13498..13812)
                     /locus_tag="ON449_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14066..15701)
                     /locus_tag="ON449_001456"
                     /pseudo
     CDS             complement(14066..15701)
                     /locus_tag="ON449_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16256..16819)
                     /locus_tag="ON449_001457"
     CDS             complement(16256..16819)
                     /locus_tag="ON449_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3934048.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16834..17877)
                     /locus_tag="ON449_001458"
     CDS             complement(16834..17877)
                     /locus_tag="ON449_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934049.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18010..18414)
                     /locus_tag="ON449_001459"
     CDS             complement(18010..18414)
                     /locus_tag="ON449_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3934050.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18647..19282)
                     /locus_tag="ON449_001460"
     CDS             complement(18647..19282)
                     /locus_tag="ON449_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3934051.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19548..20600)
                     /locus_tag="ON449_001461"
     CDS             complement(19548..20600)
                     /locus_tag="ON449_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934052.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20613..21551)
                     /locus_tag="ON449_001462"
     CDS             complement(20613..21551)
                     /locus_tag="ON449_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3934053.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         6934 a         4322 c         3466 g         7234 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     8521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     8581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     8641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     8701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     8761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     8821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     9541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     9601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     9661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     9721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     9781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    10561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    10621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    10681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    10741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    10801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    11521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    11581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    11641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    11701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    11761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    11821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    12541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    12601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    12661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    12721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    12781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    13561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    13621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    13681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    13741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    13801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16081 aataaatctt ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta
    16141 tttttttact gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat
    16201 ataaataata gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact
    16261 caaaattgat tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt
    16321 gttcttctaa aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg
    16381 gttgcccagt tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga
    16441 agcagtctac gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag
    16501 caaaaacaga ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg
    16561 tccttgctat caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa
    16621 tatcacagta aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca
    16681 aaaacttaac attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag
    16741 aaatacttcc taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt
    16801 tatgaacaac agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt
    16861 gggacgctta tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac
    16921 aggattggct ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc
    16981 ttctacaaca acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct
    17041 aggagagtcc cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc
    17101 taagagataa tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat
    17161 tgtcacggtt ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt
    17221 cggcaataga tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag
    17281 ttgatcattg ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag
    17341 ccaataagcg attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg
    17401 taaagcggct actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt
    17461 tgtatcatat actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg
    17521 ctgtcctgtt tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta
    17581 ggcagaaata aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca
    17641 aacctcattt tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt
    17701 atattgtttt gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt
    17761 tttttcaata tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta
    17821 aacaagaagg ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt
    17881 tttgcctcct tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac
    17941 atgtttcttt tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt
    18001 aagaatccct tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga
    18061 gtatgggcaa aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc
    18121 tggcaaagaa acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa
    18181 agatacccgt gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg
    18241 aaccgcgccg ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc
    18301 ttcactatgg attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac
    18361 ttcgccatca cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat
    18421 catcctttct ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt
    18481 aatgcttgca ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt
    18541 atctatacaa aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca
    18601 acaccaaata tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt
    18661 gaaaaaaact taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga
    18721 agcccaattg cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa
    18781 gacctgcaat tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt
    18841 gaccactttg acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc
    18901 ctagcccaga acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat
    18961 tgatactgaa taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa
    19021 caacaagtat tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg
    19081 aaagaacaaa aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac
    19141 tatctacgtc accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa
    19201 ggccaaggat atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac
    19261 catatttttg caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc
    19321 aacaatcaaa ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat
    19381 caataacata aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc
    19441 agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga
    19501 catctcactt acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat
    19561 ttgaagactt cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt
    19621 agtgccgcaa aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct
    19681 gcatctaaag aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc
    19741 gtacctgaaa tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa
    19801 ggaataagaa tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa
    19861 gcaatcgcaa ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa
    19921 gtggttgcag ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc
    19981 attccgattg ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc
    20041 aagatagaca ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca
    20101 gaagtaattg ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct
    20161 acaacaatcg gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc
    20221 agcccgacag caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca
    20281 acgtaaacac ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa
    20341 gaagctccgg ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa
    20401 ccaccaatga tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc
    20461 gcaaagggat acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat
    20521 ccaataatga ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat
    20581 attttttgtg aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt
    20641 gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct
    20701 tttctagaaa ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag
    20761 tcttgataca ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa
    20821 tgtgcaatga cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac
    20881 tcattgacaa tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata
    20941 ttaacgtcta agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa
    21001 atagaatagt gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct
    21061 gctaattttt ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca
    21121 cttccaaata atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca
    21181 atatagatat tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt
    21241 aatagacata agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt
    21301 tccagctgca tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact
    21361 tcggattgaa gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt
    21421 aatccatgtt ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg
    21481 ttcccacttt ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt
    21541 gctattttca ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc
    21601 acaacctcgt tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat
    21661 tttatcagca aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag
    21721 actagcaata agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag
    21781 agggggcatt aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt
    21841 ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta
    21901 ttatttatta aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttct
//
LOCUS       DAJSVP010000038        21739 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSVP010000038 DAJSVP010000000
VERSION     DAJSVP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.038"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            463..669
                     /locus_tag="ON449_001463"
     CDS             463..669
                     /locus_tag="ON449_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934054.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            865..1032
                     /locus_tag="ON449_001464"
     CDS             865..1032
                     /locus_tag="ON449_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3934055.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1058..1402
                     /locus_tag="ON449_001465"
     CDS             1058..1402
                     /locus_tag="ON449_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3934056.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1407..1688
                     /locus_tag="ON449_001466"
     CDS             1407..1688
                     /locus_tag="ON449_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934057.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1791..2105
                     /locus_tag="ON449_001467"
     CDS             1791..2105
                     /locus_tag="ON449_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3934058.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2083..3777
                     /locus_tag="ON449_001468"
     CDS             2083..3777
                     /locus_tag="ON449_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3934059.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3797..4975
                     /locus_tag="ON449_001469"
     CDS             3797..4975
                     /locus_tag="ON449_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3934060.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4938..5624
                     /locus_tag="ON449_001470"
     CDS             4938..5624
                     /locus_tag="ON449_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3934061.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5624..6784
                     /locus_tag="ON449_001471"
     CDS             5624..6784
                     /locus_tag="ON449_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3934062.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6794..7669
                     /locus_tag="ON449_001472"
     CDS             6794..7669
                     /locus_tag="ON449_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934063.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7666..7977
                     /locus_tag="ON449_001473"
     CDS             7666..7977
                     /locus_tag="ON449_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934064.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7967..8320
                     /locus_tag="ON449_001474"
     CDS             7967..8320
                     /locus_tag="ON449_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3934065.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8310..8711
                     /locus_tag="ON449_001475"
     CDS             8310..8711
                     /locus_tag="ON449_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934066.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8704..9108
                     /locus_tag="ON449_001476"
     CDS             8704..9108
                     /locus_tag="ON449_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934067.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9120..9725
                     /locus_tag="ON449_001477"
     CDS             9120..9725
                     /locus_tag="ON449_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934068.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9745..10107
                     /locus_tag="ON449_001478"
     CDS             9745..10107
                     /locus_tag="ON449_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934069.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10110..10292
                     /locus_tag="ON449_001479"
     CDS             10110..10292
                     /locus_tag="ON449_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934070.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10309..13740
                     /locus_tag="ON449_001480"
     CDS             10309..13740
                     /locus_tag="ON449_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3934071.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13791..14528
                     /locus_tag="ON449_001481"
     CDS             13791..14528
                     /locus_tag="ON449_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934072.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14538..16829
                     /locus_tag="ON449_001482"
     CDS             14538..16829
                     /locus_tag="ON449_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934073.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16853..18979
                     /locus_tag="ON449_001483"
     CDS             16853..18979
                     /locus_tag="ON449_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934074.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18996..19145
                     /locus_tag="ON449_001484"
     CDS             18996..19145
                     /locus_tag="ON449_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934075.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            19142..19588
                     /locus_tag="ON449_001485"
     CDS             19142..19588
                     /locus_tag="ON449_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934076.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19590..19727
                     /locus_tag="ON449_001486"
     CDS             19590..19727
                     /locus_tag="ON449_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3934077.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19765..20058
                     /locus_tag="ON449_001487"
     CDS             19765..20058
                     /locus_tag="ON449_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934078.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            20055..20279
                     /locus_tag="ON449_001488"
     CDS             20055..20279
                     /locus_tag="ON449_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3934079.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20276..21301
                     /locus_tag="ON449_001489"
     CDS             20276..21301
                     /locus_tag="ON449_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3934080.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            21491..21564
                     /locus_tag="ON449_001490"
     tRNA            21491..21564
                     /locus_tag="ON449_001490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21525..21527,aa:Ile,seq:cat)
BASE COUNT         7635 a         3299 c         4542 g         6263 t
ORIGIN      
        1 tttatacatg ttgtcttgca catccttgtt ttatatttca gcggaccact cgctgataaa
       61 taaaattata cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt
      121 aaatagaaca aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga
      181 taatctagca tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa
      241 ttatcaaagc aatgttattt tgttgctgtc tattgttttt taattattca cacgataact
      301 aaaggtgggt gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat
      361 gtctatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt
      421 ttatagtgtc actgtggcgg aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc
      481 ggcaaccatg caaggttcga ttccttgcca gcgacattaa atgcctatga cggttacgac
      541 taccgaaaaa agatcgttaa gaagctatac ggtgctacgt acggcaatgt agtaagtgtg
      601 ctatctgtac accaccaagc ttcggtcact gtggcggaag tagaagacgc agcggtaaat
      661 ggcgagtagc ctcgtgagag cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg
      721 actttaagca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
      781 ttttgtgcat cgtgcaagtt actattacat attagatcac tctttgagtg gtctttttat
      841 ttttgcacaa aggaggaaac aacaatgtat agaccacaat acttagaaca gaagtatgaa
      901 gtaatcactg ttcataaagg taatggcgaa aaagtatatg agtatagaag accaataaag
      961 agcgatacat ataaacgaaa ggaaaacaat gaagttattc cattgtatgg caaaagaata
     1021 gctaagcatt aaataagatt gcgaaaggag acggaacatg accgaggaat tctatagatg
     1081 gctattacag ttgacaagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg
     1141 caggcttaga gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact
     1201 aggtaagtat catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga
     1261 cttagcttta gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca
     1321 tggcagatat cttacagcat tagataaaca agagaagaag atagaaagct ttgctaactt
     1381 cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata
     1441 cagaaaagat tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg
     1501 ttcgctatgt aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga
     1561 atcaagtaag agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg
     1621 gatatagtat tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg
     1681 tgagataacc cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg
     1741 ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa
     1801 acgaattgtc tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg
     1861 aaagtcagaa aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat
     1921 gggaaacggc gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg
     1981 gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt
     2041 tggataaact tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt
     2101 tataagcata ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa
     2161 gagcaattat tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac
     2221 ttcgataagc agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa
     2281 ttaattccgt gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat
     2341 aaaacactag tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt
     2401 tttgcttctt ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat
     2461 attgatatct atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa
     2521 gtaattaaag atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt
     2581 caaaatatcg ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat
     2641 gggaagcgac caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg
     2701 ataaatgtgg ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca
     2761 actaatggtc atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt
     2821 ctttctggag aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt
     2881 atttgtcgct tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt
     2941 ccaactatta attacaatgc agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg
     3001 caacgtaatg ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa
     3061 gatacacgat ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt
     3121 cctgaaaaaa tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc
     3181 agcgttgggt tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc
     3241 cacgaatcag cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat
     3301 caaggaaaat cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg
     3361 tttcttgaaa tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca
     3421 aaaatattga agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga
     3481 tctgttacac atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta
     3541 ttttttggtg acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt
     3601 tctaatggaa atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt
     3661 ttttcattcc ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca
     3721 gtcacaaata gctcagtaga aaatacagga actggattta ctcctctagt attctaactt
     3781 gaaaggaggt gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc
     3841 aacggttcct ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt
     3901 tgcgattgaa acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc
     3961 atatgaagat ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa
     4021 taagaaaaat aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa
     4081 tgaagtattg atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg
     4141 ggatgtcaca gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat
     4201 agtccttgat agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca
     4261 aattaggcaa ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat
     4321 gaatgtttac aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc
     4381 gcaagacaat acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt
     4441 tatggaagct gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga
     4501 tttcagcgga aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt
     4561 tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg
     4621 gttatcggat caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat
     4681 tcaggatgcg tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt
     4741 acgtgtggat acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga
     4801 tgtaggcatt agaaatggag tatttacaat caacgaagga agagaacgcg ttggaaatga
     4861 tcgctctgat aaggcgatgg cagatgaaat atttataact aaaaacaatc aacaagtatc
     4921 gaaaggaggt gaggcgaatg acgacaatga aaacatttct agcagtaaag aatgaaggcg
     4981 cagtaccgca aatttttatt cagggattta ttggttctag ttggttcttt gaagggaata
     5041 ctgacaaggg aatcaaaaat attttggata gtctaggtga tcaagaagaa attgaagtag
     5101 taattaattc aaacggtgga gacgtatttc aagggattgc tattgggaac ttacttaagt
     5161 caaataaagc aaaagttaac gttgtgatta acggcttagc cgctagtgct gcttcaatta
     5221 tcgcaatggc tggcgatact ataaaaatct acaacaatgc acaattgatg attcaccgcg
     5281 cttccacata cggagaagga aatgtcgatg acttccgtac gattgctgac caactggaat
     5341 caattgataa atcggtaaag gcttcatata aaacacgatt caatggcaca gatgaagcat
     5401 tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga aacagctttg agttatggat
     5461 tggtcgatga aattatcgat gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa
     5521 gcgttgaaga aattttgaat gacgttaaag aaaaaagagc agaaaaaatt gctgcattta
     5581 cagcagcatt aaataaaaca tttggacaag gagatgtaaa ataatgacag ttaaaaattt
     5641 aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga
     5701 atcttttagc gcagctatgg taagcttatc taaggaaatt caggataaaa ttttagaaga
     5761 agcaacagca aaaaatcaag atcaattagt attaatgaac cgtggtcagc gtgtattaac
     5821 tacacaagaa acaaaattct ataacgaagt agtgaaaaac gaaggttttg caggggtcga
     5881 agaattagtg ccagctactg tatttgaacg tgtatttgaa gatttagaac aatctcatcc
     5941 actattgcaa aaaattactt ttgttaacac aactggtgta acagaatgga ttgtgtcacg
     6001 tggagtcaat ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag ttttagataa
     6061 tggctttgat gtaattaaca tgaagcagtt caagctatca ggttatattc ctgtatgtaa
     6121 ggcaatgctt gatttaggtc cagtatggtt agatcgttat gtccgtactg ttttagtaga
     6181 atcattgaga attgcattag aacaagcaat tgttgatggt actggtaaag atatgccagt
     6241 cggaatgatg cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga
     6301 acctattaca gctttagatg ctgtaactat gggcggtttg atggcgcgac tatcaaaatt
     6361 caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg aatccttctg atgtggtcct
     6421 aattgtgaat ccaactgatt actggtctaa agtattccca gctaagactg tactaactgc
     6481 taatggagaa tacgtacaag tattgccagt accagtttca gatttgcagt caacggctgt
     6541 gccagaagga aaagcagtta ttggggtagc ctcagattac ttcatgggtg taggatctac
     6601 actaaaaatt gaagcttcag atgaatacca ttttgttgaa gacgaacgca tttatctagc
     6661 taaacaatat gcaaacggtc aacctaaacg taacgatagt ttcattgtat tagatattag
     6721 cgctttggga actactacta caactacaaa accaacaacc acaacaacta caacacaagc
     6781 gtaggtgatc agaatgaagt atattctttg tcagccggca atcaatcggt ttaaatggga
     6841 gcttgaagtt tgtttaacta atctgaagaa actaggaatc aaagatatcg tattgctttt
     6901 cagcagacac gatgatcaga ttcctatttt ttttgagaag gaatatggtg ttgaagttca
     6961 tgtgtacgat gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg
     7021 gtggaaatat ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc
     7081 ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg
     7141 gtattgtagc gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg
     7201 agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac
     7261 tataaacact aattcaggag gcgcacagtg ggttattaac cgccctaaag cgaattattg
     7321 gaaaaaggtt tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa
     7381 tatacaaatc tggacagccg agatgtgggc acagctttgg aacatgatgt atttcaatat
     7441 tggtcctaaa gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt
     7501 taaagaagta aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt
     7561 caaagggaga tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa
     7621 aaaatgctct tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa
     7681 gtgactgaag aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa
     7741 gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt
     7801 acaacacttt ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga
     7861 tacgactgga atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt
     7921 ttcattcaac attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg
     7981 tagactcgaa gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag
     8041 aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag
     8101 aaatctttcc atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac
     8161 taagaaagta aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt
     8221 tatcgtaatc gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat
     8281 ctatttttat cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac
     8341 gtttaaataa gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac
     8401 tctttcaaga ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg
     8461 aaacaggaga aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc
     8521 taactttcta ttcagaaaat agagaagatt taacaggaga ttcactagat attatttcat
     8581 tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga
     8641 accaagatcg ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt
     8701 gtcatggcta aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg
     8761 atggaacgct attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt
     8821 tcggctattg cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga
     8881 agagggcacc aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt
     8941 ttcatcatta gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt
     9001 acttctaaaa gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat
     9061 ccaattacag aacttctgat tcgtcaattc gataaattaa ataaatagga ggaacaacaa
     9121 tggctaaaac aacaacagta acaacttttg acaatataag tattaaaaga atttctttta
     9181 attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa tggacaatta gatggcgaaa
     9241 cagaaatgca gagaattgta aaaaaatgcg gttcaacaga agtaaaatcg aaatctaaac
     9301 caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgcttca
     9361 atgggttgaa acaagatgaa cgtattaaat cgggaattta ctcttatggt cctgattccg
     9421 taggggaaga tttctctctt gctgcagaga tcgtggatga tttcgaagaa aaaaataagc
     9481 tgattggtat gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg
     9541 cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat
     9601 tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga
     9661 atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac
     9721 agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat
     9781 ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga
     9841 gattttaaca cagacgagtt tgcaaaaatg actgtgggaa aaggatccat ggatatttct
     9901 gttattcaag gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat gactgattat
     9961 atttcattcg atgaatttat cgataaatgg gattttgata tggctactgc cagctatatt
    10021 tatcaattga tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca
    10081 aataaggaaa aaaagcttca aaagtaaaaa tgccaaagct cttagttgaa acgtgggtcg
    10141 atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt ttaagcgata
    10201 tctgtttaga tgacattttg caaatggctt acaacaagag tgcttatgaa ggatggaaaa
    10261 actatgcaat aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag
    10321 agaacagaag cagaagtaac attcatagct aacgatgacg gattgaaatc tacgttaaaa
    10381 gaaatcagtg ctgaattaac taaaaataga gcagaattaa aactagaaca agctcaatta
    10441 caacagactg gttctgaatc agacaagtta ggaagtaaat tatcttcttt agaaaagcag
    10501 tatgaattac aaagtcaaaa agttgaagta actagccaac gtttagccaa tgccaaaaaa
    10561 tattatggag aaaattccac cgaagttcag aaacttgaga gagaactgat taaccaacaa
    10621 acagcgcaac aacgtttgtc aaacgaaatt gataaaacga gtaatgcact agctcaagca
    10681 aaaggcgaaa tacagacgta cgagtctaca atgcaacagt tggatagtga acaaaaaaat
    10741 gttcaagcta gtgcttctct gattgaatcc gaatacaaaa aatggcaagc aactgctggt
    10801 caatcagctt ctgaatccga gaaattagcg aaagcccaag aatatgtttc tcaacaatct
    10861 gaaaatgcag agaaaacgat agatatcctg agacgacagt tagaagctac acagtctgag
    10921 tttggcgcta catccacaga agcaatgcag atggaggcga agcttaatga tgctgaacgt
    10981 gaatttgaag agttaggaca agctgctaaa aatgtagata caactaactt ggacgatatc
    11041 ggaagcaaaa tagatatgaa taatctaatg gaagcttctg acgttttaag cgacattggc
    11101 gataagctta cagaattagg gaaacaagca gtggactctg ctaatagtgt aggtagttcc
    11161 cagagtaaaa tacaagctaa ttttggtttg actaaacaag aggctgaaga attaacgaat
    11221 gtagccagag acatttatta taaaggtttt ggagaatcgt tagatcagtc cacagatgca
    11281 ttgattttgg taaagcgtaa tttaggcgat ttaaataatc aagatttaca aaatatcacg
    11341 gaacaagcta tggtcctaga aaacaccatg ggcgctgata tggatgaaac gttacgtggt
    11401 gtaaatggct taatggtcaa tttcggcttg agcgctcaag atgcaatgga tttaatggtt
    11461 tcgggtactc aaaacggttt agataaaacg cacgaattag gcgacaatat ggcagaatat
    11521 agccaattat ggagtcaaat gggatattca gctgatgaaa cgttcggaat gcttcaaaat
    11581 ggtttagatg cgggtgctta taaccttgat aaagtcaatg acttagttaa ggaaatggga
    11641 atatcgttaa cagatggtcg atttgagcaa aacatggata tgtttagtga aagtactaga
    11701 aaagcttttg aagagtggaa aaatggcgga ggaacacaaa aagacgttat taattccatg
    11761 attcaagatt ttagcaatat ggatggtcaa tacgaccaat taaataaagc ttctacaatt
    11821 tggtctgcac ttggcgaaga taacgcgatg aaagttgtcc aatctttgac tgatgttaac
    11881 catacatttg atgatgttag tggatctgca caaaaaatga atgaagattc tactactccg
    11941 ttacaagaat taaacggaaa aatagctgaa ttaaaggatt cattagctcc tataggcaac
    12001 acaatcatag atgcactcga accagtaatt gattttctag gaaagatggc tgatgcgttt
    12061 aataatcttc cacaaccagt acaggatttc attgttgcta taggtggtct tacagcagca
    12121 tttggcatac tgttaccggc aatactagcc gtgtcatatt tatttggtcc gatgatgctt
    12181 ataattggag gaattatagc tgttatagca ggcgttattg tggcaattaa gaactggggt
    12241 gcaattactg actggtttag tggtttatgg aaaaaattta ctgattggtt gggtgatact
    12301 tgggaaagta taaaagacgg agcctcatca gtttgggatg gggttaaaga aacctggtct
    12361 ggatttgtag attgggttca agatatttgg caaggagttt ctgattggtt tggagagcta
    12421 tggagcggat tagttgaagg agcttccaac atctggcaag gagtccaaga aacttggcaa
    12481 acattcgttg attgggtttc aaatatttgg aacggagtca aagaagtatg gtcgattatt
    12541 tgggcagaca ttgtaggaat tgttcaaata ccatggacat taataacgtc attgattcaa
    12601 gctggtatta atattatcgt gggtattttt gatgtagctg gacagttatt gggcgcagct
    12661 tggcaagctg tttggacacc tatttctgat ttccttaaaa atacttggga tactatgaca
    12721 caatggataa gtatcgcttg gaatgggatt gtaactacat tccatactat atttgatcca
    12781 gtagtggcat ggtggaatgg tatatggaca gctattagta ctacggcttc aaatatttgg
    12841 aattcaatta gtgcaacagc ttctagtatt tggaacagta tcaagaatac aatcactagc
    12901 ttggtacaag cagctgctac agtaattcaa aatatttggt caactgtatc tagttggtta
    12961 ggtggaattt ggaattcaat cagctctaca gcatcaaata tctggaatag cgtgactagt
    13021 agtataagca atgctataaa cgcagctaaa agtgccattc aaagtgtttg gaatagtata
    13081 tcttcgtgga ttagcggaat ttggaacggt atcaaaaata ctgctttgaa tctttggaat
    13141 ggaattacaa gcactattag ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt
    13201 tggtccaatc taacaggtat tgtttccgac atattcaata atgttaaaag tacaattgct
    13261 aacatatggg aaggcatcaa aaagactgtt agcgctccaa ttgattggat tagagacaaa
    13321 atcagtggca tttttgataa tttgaatatt tctataccac atattccgtt accggaattt
    13381 atcatggaag gcagctttaa cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg
    13441 catgctaaag gaggtatctt tactaaacca actttactag gtggaatgaa cggtgtcggt
    13501 gaagcaggac ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat
    13561 cgtatcttga gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac
    13621 tacgaattca atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact
    13681 caacaaatca ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa
    13741 acaggagagt atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt
    13801 gtatacattc tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa
    13861 gaccaaccat accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag
    13921 gtggcttagt acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt
    13981 tagaggatgt ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt
    14041 taaactatgc aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga
    14101 ctatcgatat tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata
    14161 ttactgcgaa accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac
    14221 agtcatttaa cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc
    14281 cttcagctac ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag
    14341 tagcaggaac tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg
    14401 acggagatat tttagatcgg tccaacaaaa tgaagaccat gcaatatccg acattgcaag
    14461 tggatatcaa tcattttaat tgcactggtt tgagcaaaat acaaatttat cgtaacgggt
    14521 taaggtaggt gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga
    14581 ataatttagc tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa
    14641 cagctactgt tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa
    14701 acggaactaa tgcagatttg attgtagacg gacgtattat tcagtgttat gtagatgaaa
    14761 atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga
    14821 tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg
    14881 acagcggaac agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga
    14941 aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga
    15001 agatagaaaa ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc
    15061 gatttcatgg tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta
    15121 cggatcataa aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg
    15181 atgctcaaag tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag
    15241 acgagatcac tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg
    15301 gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag
    15361 ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga
    15421 ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag
    15481 tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga
    15541 gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa
    15601 acgtcaaaac aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg
    15661 ccattgatca attgaaaaac ggtggcgaaa tcagtgattt actcaatgat attgtagatc
    15721 atcaaactga tatgattact ggtcaaaatg gtggttatgt tttattagat cctaaagaag
    15781 cgcctagtcg tattttgatt atggacacac cagataagaa taccgcaagg aatgttttac
    15841 aaatcaacaa tgctggtatt ggtttttcta aaactggcat taatggaaca tatgaaacgg
    15901 catggacgtt agatggcgga ttcaatgcct cgtttattac ggctggtgaa atagtaggaa
    15961 ttactattag aggtactaca ttaattagtg atggtgctga ttatagaaca agtattgcta
    16021 atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac
    16081 gtgattatgt aagtgctgat gccggtattg tatcatacac catgaaaact ggtggtggtt
    16141 tcatgattag aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca
    16201 gaccgtttct atcttttggt gcgcccaatt tcaggtatag caatgctagt tatgtaactt
    16261 ctggcgacgg tagttcttta agcattaatg gtagtgcggg taactcatgg gaatttaagg
    16321 tagctggtag gactatgaaa tttactagtg atggtatgct aacgttacca ggttgttttt
    16381 ttggttcatg ggaagatggg aaacttgcta ggtttgaaca atcaacggta caagtatata
    16441 aagattttac tgttagaggt actaaaaact caactgtacc aacagaacat tatggacaac
    16501 gactattgaa cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta
    16561 caggtgacaa tggtaaagtt cgtgttgata ttgaccccat gtttgctgaa acagtaaatc
    16621 taagtcggta tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg
    16681 aagatattga ccatttcatc attgaaacta gtaagccaaa cgtattagtt agatggaatt
    16741 tagtggcaca ccgtctaggg tatgaagata ttagattaaa agaggataca gcatatgata
    16801 gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag
    16861 cagtttatat aatttggctt tagatttcag caaagaatta aactacacca aagctattat
    16921 ggctcgtcaa ggtgataaag ggattacggt gacggttaaa ccgtttctaa atggcttgca
    16981 gatggatacg agtggcggaa catttacttt aaaaggaaca acaccatcta accgttacgt
    17041 agataatgtt gcaactagtg taactagtga agaagtcacg ttttctcttg atggcacatt
    17101 tatgagtgaa gcaggatatt ataaacactg ctacgtagaa tatagaaaag acaatcaaat
    17161 tttaacaacg caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca
    17221 agccgatgaa tatgtttcgc aattagaaga gttgattcga aagtacaacg aaacttttga
    17281 tgcttttatg gctgaaatca aaggtagagt ggatagctta aatcaacaga ttactgattt
    17341 aactggtcaa gctaaaacgc tacaagacaa gttagatgct ctgaaagaag aaatttctaa
    17401 gttaggtaac ttacaagtga tgtacagtaa cagcatcgac ttcggggact atgattattc
    17461 ggagaacccg aatttaatgc catatattac agaaccttgg gttggacctt tgttaggtaa
    17521 tggacataca gttaaagata gtgttaaaag ggttataact catactaaaa ctagaacagc
    17581 taattccggc gacatattat cgcttggctt aggtattccg tgtacagcgg aagctaataa
    17641 taggtatctt ataaccactt tacgaccatc tacgacatac acattaagcg ttactatgag
    17701 tgtcggttca gattggactg gagagaccaa tactataggg gttaggctta gatatttaaa
    17761 tgaacaaggt ggtatagaac tgcccattaa tgcattaata ccagcaaatg tagaacgcga
    17821 taaaatggtc actcatacat ttactggtat aacaaaagat aatgtaacta gcataacaaa
    17881 ctgttatgtt gaaatattct cacttaatag tgaatataaa ggaacggtta gtgtcagcta
    17941 tgacgttaag ttaaaagcac actaccctaa cttactagat ggcccttact ggttaggtaa
    18001 agttccttta ggcgagaata ttgctgaccc tacagttgtc tttccacaca aaactagtga
    18061 atatatggtt tatggtagac gcaatacaga aaactatatc gctgaccaaa catataccat
    18121 atcaatgaaa gcaactaaac taacagttca atcgtttgcc gtgtatattg ctgctggccg
    18181 cgttaaagta ggagacatga agcctactga ggggttagct aatacatggg agttaacttt
    18241 tactgtaact aagcaacata tagatagtgg ggtaactaat tatctggaaa tataccaata
    18301 cccgtcagct acaaaaggag ctgttcaaat tgagtggctc aaactagaaa aaggcaatac
    18361 tcgaaccccg aatattagtg agtataaata tcgtggtact ggtatgcgtg attcaaacaa
    18421 tccaaaagat tatgtttggg atctagcacc agaatatgtc gaagataatt tggccacaga
    18481 tattaaaatt tctgaaatta ctggtaaagc aaacaattat accgatggga aagtatcgga
    18541 gattaattcg cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca
    18601 aacaaaagcg aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg
    18661 cattaatgat acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa
    18721 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaacgcag
    18781 tggtggaaat gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt
    18841 tgagcaactg gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg
    18901 tgcagatgga gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc
    18961 aattacttac acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg
    19021 aaccttacgg attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca
    19081 cagaagtcga accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata
    19141 aatgaaaaac atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga
    19201 cgacatgctt gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga
    19261 tggcgaaccg ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat
    19321 tttagcgaac gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt
    19381 agaaaatgag aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc
    19441 aattaaacag gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc
    19501 aaaaatgatg cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact
    19561 aaatccagct tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt
    19621 atgattgggg ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg
    19681 aagaaaaagc agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt
    19741 tattttgatt caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga
    19801 tcaataaccg ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc
    19861 tcgaagatta ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat
    19921 ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa
    19981 agattagata cacatgcaga aaaattggta gaacacacgc aacaaattaa aactttattt
    20041 agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa
    20101 ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca
    20161 cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg
    20221 tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa
    20281 aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat tacccattaa
    20341 tacgtttgct tatactatta atgacgagta taatttagcg ccgaatcaag gagactccag
    20401 attagcaatt cctaacaaaa ttattttgca cgaaactgga atagatgcac cagcaagaaa
    20461 cgtagccgcc aacatgaaaa ataattataa cggaagcaat tcttatacta cagatgttat
    20521 tggtgacggt gggattgttt accgtgtggg tgagcaagga tatgtttcgt ggggagctgg
    20581 taacgctaat ccttatgcgc ctgtacagat tgaattacag cgcacatatg ataaagcatt
    20641 gtttgaaaaa aactatcgag cttatattga atatacaaga gatagtgcaa aaaaatatgg
    20701 aattccattg actcttgatc aaggaacttc tttatttaca aaaggaatca tttctcattt
    20761 gtgggtgaca aattatgttt gggggaacca cacagatcca tatggttact tatcgcaaat
    20821 gggagttagc aaagaaaagc ttgcttatga tttagctcat ggatttaccg atgaaaatcc
    20881 aacaacttct gaaaacaagc ctgtcattga tccaacacga gctggtgcag ctaatcctac
    20941 gctgacagat ggaacaaatc acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa
    21001 cttgcatgtg gctggatggc acattgctaa ctataaatac gagtatattt tcattatgga
    21061 ctacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata gaccagatgt
    21121 aaaccaagct tataatactt ctggaaatgt tggttatcat gtatctttca atatgcgtaa
    21181 ttttcctagt aagaaagtct atgtaatgat gcgggcaacg aatgatccag agggaaacac
    21241 taaaggcggt gcgcaagatt tccatgacaa acgttggtat ttaaatattc ctaaacgata
    21301 aaaatagctc ctcgttgagg agcagtacat aactatattg acaactataa aaattattcg
    21361 ataaaatagt gatgttatcg catatcttca ctatcaccca taaatagtca cactccaagc
    21421 tatgcgataa caggtttgtt gccacacatt ctactggttg attgtttatg gctttatgtg
    21481 gcaacaacca gtacccttag ctcagttggt cagagcagac ggctcataac cgtccggtcg
    21541 taggttcgag tcctacaggg tacattaacg tagccatttg aatcgttctg tgttagaatt
    21601 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
    21661 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata cttctgtata
    21721 tttgaaaagt tttactttg
//
LOCUS       DAJSVP010000039        21563 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSVP010000039 DAJSVP010000000
VERSION     DAJSVP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.039"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..289)
                     /locus_tag="ON449_001491"
     CDS             complement(<1..289)
                     /locus_tag="ON449_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCR3934081.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVT"
     gene            complement(376..1080)
                     /locus_tag="ON449_001492"
     CDS             complement(376..1080)
                     /locus_tag="ON449_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR3934082.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1280..1546
                     /locus_tag="ON449_001493"
     CDS             1280..1546
                     /locus_tag="ON449_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934083.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1559..1717
                     /locus_tag="ON449_001494"
     CDS             1559..1717
                     /locus_tag="ON449_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934084.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1714..1944)
                     /locus_tag="ON449_001495"
     CDS             complement(1714..1944)
                     /locus_tag="ON449_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3934085.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2016..2171
                     /locus_tag="ON449_001496"
     CDS             2016..2171
                     /locus_tag="ON449_001496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934086.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            2328..2483
                     /locus_tag="ON449_001497"
     CDS             2328..2483
                     /locus_tag="ON449_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934087.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2480..2704)
                     /locus_tag="ON449_001498"
     CDS             complement(2480..2704)
                     /locus_tag="ON449_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3934088.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2971..3306
                     /locus_tag="ON449_001499"
     CDS             2971..3306
                     /locus_tag="ON449_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934089.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3539..4480
                     /locus_tag="ON449_001500"
     CDS             3539..4480
                     /locus_tag="ON449_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCR3934090.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4482..5372
                     /locus_tag="ON449_001501"
     CDS             4482..5372
                     /locus_tag="ON449_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCR3934091.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5415..6215
                     /locus_tag="ON449_001502"
     CDS             5415..6215
                     /locus_tag="ON449_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3934092.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6230..7081
                     /locus_tag="ON449_001503"
     CDS             6230..7081
                     /locus_tag="ON449_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3934093.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7078..7395
                     /locus_tag="ON449_001504"
     CDS             7078..7395
                     /locus_tag="ON449_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934094.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7388..7570
                     /locus_tag="ON449_001505"
     CDS             7388..7570
                     /locus_tag="ON449_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934095.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7602..7691
                     /locus_tag="ON449_001506"
                     /pseudo
     CDS             7602..7691
                     /locus_tag="ON449_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7766..8233
                     /locus_tag="ON449_001507"
     CDS             7766..8233
                     /locus_tag="ON449_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCR3934096.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            8761..8833
                     /locus_tag="ON449_001508"
     tRNA            8761..8833
                     /locus_tag="ON449_001508"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8794..8796,aa:Lys,seq:ttt)
     gene            9219..9479
                     /locus_tag="ON449_001509"
     CDS             9219..9479
                     /locus_tag="ON449_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934097.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            9522..9695
                     /locus_tag="ON449_001510"
     CDS             9522..9695
                     /locus_tag="ON449_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934098.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            10084..10917
                     /locus_tag="ON449_001511"
     CDS             10084..10917
                     /locus_tag="ON449_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HCR3934099.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            10910..12325
                     /locus_tag="ON449_001512"
     CDS             10910..12325
                     /locus_tag="ON449_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR3934100.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12337..13866
                     /locus_tag="ON449_001513"
     CDS             12337..13866
                     /locus_tag="ON449_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3934101.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            13784..14836
                     /locus_tag="ON449_001514"
     CDS             13784..14836
                     /locus_tag="ON449_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR3934102.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14838..15155
                     /locus_tag="ON449_001515"
     CDS             14838..15155
                     /locus_tag="ON449_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934103.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15282..15905
                     /locus_tag="ON449_001516"
     CDS             15282..15905
                     /locus_tag="ON449_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCR3934104.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            15920..16810
                     /locus_tag="ON449_001517"
     CDS             15920..16810
                     /locus_tag="ON449_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HCR3934105.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16833..17048
                     /locus_tag="ON449_001518"
     CDS             16833..17048
                     /locus_tag="ON449_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934106.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17060..17401
                     /locus_tag="ON449_001519"
     CDS             17060..17401
                     /locus_tag="ON449_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934107.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17401..17772
                     /locus_tag="ON449_001520"
     CDS             17401..17772
                     /locus_tag="ON449_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934108.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17765..18160
                     /locus_tag="ON449_001521"
     CDS             17765..18160
                     /locus_tag="ON449_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR3934109.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18162..18539
                     /locus_tag="ON449_001522"
     CDS             18162..18539
                     /locus_tag="ON449_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934110.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18540..19148
                     /locus_tag="ON449_001523"
     CDS             18540..19148
                     /locus_tag="ON449_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934111.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19148..19489
                     /locus_tag="ON449_001524"
     CDS             19148..19489
                     /locus_tag="ON449_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934112.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19731..>21563
                     /locus_tag="ON449_001525"
     CDS             19731..>21563
                     /locus_tag="ON449_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR3934113.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFV"
BASE COUNT         7719 a         3222 c         4452 g         6170 t
ORIGIN      
        1 tagtgaccag agatgtgtca tatttttgat taatttcatc gtctaaccgc tggctttcag
       61 cattaagaat atcaattttg ttttctagcg ttgacaagtt tttattcaat ttacttatgt
      121 ttttctcttt ttcagatata aacgtttccc tagcatttaa ttcttgagaa tattccttta
      181 ccttactctc taaggcagaa gttttatctt ttaatttttt agcttcagat aatttgctac
      241 tacccataat tattaaaact attccaacta aaaacaatag tactcccatt caaaacgcct
      301 cgcttctttt ataaaataat tgtcaaaatc atataaaccc ttaccgaagt tgacggctcc
      361 ggcatgggaa tttttttaaa atacaacttt tccgataatt ctaatctgtt ctggcgatac
      421 aattctatct tcgtataagt cgtttataga acgtaaaatg attgtttgat tttcgtaatc
      481 tttatatatc tctttacatg taactccgtc accacctaat tccacaatag caatttcccc
      541 atcttctacg tccggttgtt ctttaacgaa tacgaattgt ccattcctga taacaggttc
      601 cattgaatcg cctataatag gtactgcgaa gtcagcagca cttggaactc tctcaaattc
      661 ttcctcttcg acaaccgtat caccgtaagt aagttctgtt ggattggcag ccgaagccga
      721 attgtgaaca gttgggattt tttctttttt aggaaaatct aggacatttt cttgtttttg
      781 ttcttctaat cgattacttg caaattcata ggtagcaact ttattttcgt cgttaagctg
      841 attatatata ggaaggatat tatatatctg attagatata gataaatcaa tattcatcaa
      901 atcatctata tcaacattga atatcttagc tatctcattt aaggttttta attttggtgt
      961 atattttcct ttttcccatt cgcttattga ggatgaactt tttcttccta gtttttcggc
     1021 tagttcttgt tgatcaatat tatacttttc tctaagaaac tttagatttt cagcgaacat
     1081 agtaacacct ccttgagtta attgtaacac cgtttcggaa aaaatgtaac aggtttctta
     1141 gaaaatattt tagtgttttt aaagaagtat ttacgcaaaa tagaaatcga tttcaggaaa
     1201 actgaaatat gcacttgact tcggaaaaac cgaagtatat aatttagtca gaaagttaaa
     1261 gagaggaggc gaataaaata tggataccgc gaagaaagaa aaatatacat tgagacaatt
     1321 aagagcttta aagggttatt caaaagaaga actgtcaagg aaaagcaaag tgacatcaag
     1381 aacaatattt atttacgaaa acgatgtaga taaaatgagg aacggaaagt acgccactct
     1441 agaaaaaatt gctaaggcat tgggtgttag agtttccgat atttttttag accctgattc
     1501 ggaaaaaccg aaatcaataa caggtgaaac aaaacaagtc agctaggagg tgtgaatagt
     1561 ggaagagaag aatataaaag taccgactct tgaagaatat acaaaaattg ttgtcgaaac
     1621 agacgagcag aacccgaaaa caatcgcagt tattacggcg aatgatattg aatcatgcga
     1681 aggttttcga gttcgaatga cccctagata tgattaatgt tctttatcac gtggaggtat
     1741 aggatcgttt ccaaaactat cttttgattg tatttgtcca ttcttttttt ggacaataaa
     1801 ctctgattgc tgttttttag aaatcttttt accatactca acagcatctg atttattatc
     1861 aaatagtttt gtatcttcgg aattaccttc accacgtaca gcccatttgc catttctagg
     1921 agatacccat tgatttttac ccataacaat tcacctcgct ttcaagttaa ttctattaag
     1981 aggtgaaaag aaaacaaact gatggaggtg agattgtgag taacaagcca aaaatcactt
     2041 tgaatggcac acctaatctt caaagattag cagatgaaat ggctagaata cgttctagac
     2101 attggggagt tactgtggtg gcaaccataa ttgaacaaaa agaaaagccc acacagccgg
     2161 caagcaagta gtgggcacca tagaacaata atattatatc acacactcgc cacttccgaa
     2221 aagaggagtt gctgctctcc tttcaagttt gccaaagtcc acgtttcttc attttggcaa
     2281 ctcctcttct tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat
     2341 caaaaatagg gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag
     2401 gggaaaaagt agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc
     2461 gattaactcc taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac
     2521 ggctttgaat ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa
     2581 tctctctagc gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat
     2641 ttccagcacc tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc
     2701 ccatagtttt cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca
     2761 ataaacgaaa ggagggatca aatgacatta caagagcatc aacacttgat gcgaaaacta
     2821 aatcaggaat accacgagga aatgaaacct agattgctag gggaacaatg gcgtgaacgc
     2881 cagaagaagt ggttagaaat aaaaaagtga ctcagccgtc caaagcaatg agtcacaaac
     2941 caaaaattat acctaaggag attttatcac atgaataaga aaattgaaaa tcttatcgaa
     3001 gaattgaaac gtgaatgcca aaagcaaggt gtttctatta tttgtacagc ccaaaaagaa
     3061 ggggaactta aaagtctcgt ttatggtgag acaacagaaa ttttactttg tcttgctatg
     3121 caagaagaac atttagatga aaatctccca ttgtcagcac atattatgcg tagaattgct
     3181 gtggatgctt acgaacaagc gaaaaatgaa gaagaaaatc aaccttcaaa tcatactttt
     3241 gttattaata acaaggaaga tttagctgat gtgatgactc gtattctcaa gggggaattt
     3301 caatgatgaa tgaaaaaaca attgatctac tcattatttt atttttcacg ttagtgattg
     3361 tagcgaaaag ttatgtaatc tttggcttgt tattcgcaat taccatgctt gtgctttgcg
     3421 tgttagcaag cagaaaagag gttaaacatg aacgagttag caaatcttga caactattta
     3481 actgatcctg attatgctaa accaccatat gaagcgccaa ttgatgagga ggatgaagat
     3541 gagtaaatct accttagaaa tgagccatca agaatggctc gaagaccgca aaagaggtat
     3601 tggtggctca gatgttgcaa ccgtacttgg attaaacaaa tacaaatctc cttatcaatt
     3661 gtggcttgag aaaacgggtc aaattgaatt gaaagattta gaaagcgaac cagcttattg
     3721 gggtaatgtt ttagaagaag tggttgctaa agagtttcag gaacgtacag gcaaaaaagt
     3781 acgcagaaga aaccaagtat ttgaacatcc attacatcca tttttaagag caaatattga
     3841 tcgggaagta gtgggagaaa atgccattct ggaatgtaaa acagccaatc aatttctcgg
     3901 aaaagagtgg gaaggtgaag aggtaccgct tagctatctc tgtcaagttc agcattacat
     3961 gaacgtttta aacaaagact attgttatat cgctgttttg atcggtggac aaaaattcat
     4021 ttggaagcgg attgaacgag atcaagagct gatcgataca atcactgaac aattagtaga
     4081 gttttgggaa acgaacgttc ttggaggtat cgagcctgta attgacggaa gtggagcgac
     4141 tgctgacttc ttaaaagaaa aatatgcaga tgtagaagaa aatcaaacag ctctaccatc
     4201 acgttttgat gaacttatcg agcaaaaaag agaactcaag cggacgaaaa aagaaattga
     4261 atcatctatt cgtcaagtag acaacgagat catcagtgag ctaggtaaac gtgaagccag
     4321 tatcggtatc acacaaagaa acattatcag ctggaaactt gttcgtacaa gacggatgaa
     4381 cacgaagaaa ctagcagaga aatatccaga tgtcgcaaat gatgaagaga tttataacgt
     4441 tactgaatca agaaggctaa ccgaaaagga gatcaaataa tatggcaaca aatgaatcgt
     4501 taaaaaatca attggcagaa aagccacaga aacaagttgc accaggacag ttagggctta
     4561 aagctctaat gaatacacca acaatgagaa agaaatttga agaagtactt catgacaatg
     4621 ctaatgcttt tatgtcgaat gttatgactc ttgtatctaa tgacagttat cttgcagata
     4681 gtgaaccgat gtctatcatg agtggtgcgt taactgctgc aacattaaat cttgggctag
     4741 ataagaattt aggttatgca tatttagttc cattcaatag taaaaacaag caaacaggaa
     4801 aatgggaaaa gaaagctcaa ttcatgcttg gctataaagg atatatccaa ttagcccaac
     4861 gatcaggtaa atacaaagca ttaaatgtga tcgaagttta cgaaggagaa ctaaaaagct
     4921 ggaaccgact gacagaagag tttgagtttg atccaaatgg tagaacgtct gatgaagtca
     4981 ttggatatgt tggctatttc gagttactga atggattcaa aaaaactgtc tattggacca
     5041 aacaagaaat cgaagctcat cggattgcta acaataaaga tcgcgataag acaaagttaa
     5101 gtggtgtgtg ggcatctgat tacaatgcaa tggcacgaaa aactgttttg agaaatcttc
     5161 tttctaaatg gggaatcttg tccatcgaaa tgcaagaagc caccacatcg gatgagagag
     5221 tccaaagggt tcaagaagac ggcagcatta ttgctgaaac agaagttgaa gaagatattc
     5281 ctgaaagaaa agaagcagag gttatatctg aagaaaatga agatgtacaa actggattat
     5341 ttgatgcatc taatccgccg ttaaacaaat aatgagggag ctttctccct tgcttttcta
     5401 gaggagaaat acgaatgaac acaggatatg tcaaattaca tcgaaaagtg atgaattcat
     5461 tcgtttggac caatccttat atgtataaat tatggagctt gtgtttgatg aaagcgagtc
     5521 atgagaatcg caagatactt tttaatggaa aagagataca agtgaacagc ggagaattcg
     5581 tcacagggcg cgatgctatc acatctgaga tgaacgaagg cgtgaaacgt gaacatcaag
     5641 tgaacagcgg ttctgtatgg agatggctaa aacaatttga aaaaaacgga atgttgaaca
     5701 tcaaatcaac tacgaaatac agcgttatat caataaaaaa ctggtctttg taccaaagca
     5761 gtgaacaaca aatgaacatc aaacgcacaa caagtgaaca acaagtgcac acaaacaaga
     5821 atgaaaagaa ttataagaat gaaaagaata ataaaacatc atcgtcgcaa ccacgcaaaa
     5881 agcgtgttta cgacaccgac tcagtttact acattctcgc ggaggagtta ttcaaacaga
     5941 tttgtcagaa tcaggaaatc aaaaagccga atctgcaaag ttgggcagat aacattcgga
     6001 aaatgatcga gatcgacaag cggaccgaaa accaggtacg gggaatgatt gaatggagtc
     6061 agcacaatgt gttttgggca tcgaatattt tatctgctaa aaaattgcga gaaaaatacg
     6121 acacaatggc agcgcaagca aatcgtgatt ataaaacaaa acaaactaaa acgcttgaat
     6181 acgagaaatt tagcacagat gagttgccta tttgagagga ggcgtaggca tggaaactgt
     6241 tggggaaatc atggaaaagc tgatacagaa agtgcttgtc caacgtggcg aatgtcctga
     6301 atgcggacag cctttgtatg gatggcgtac gaagaaccct gatggctcag aacgttgtaa
     6361 gccaacctgt atgcaatgtg ggtataaagc attgcgagtt caggaagact tacaaacaga
     6421 acgaatttac aacgagagcc tgaaagcaag agcaatcaat tttttcaaag gtggttctgt
     6481 tgtgcctaat caagcgttgt ttgattgcac attgcagaat tatcaaattg tcgatcaaga
     6541 aacaagacaa gcggttgaag taaccaaacg ctttgttaat tcagtcttgt taggaaatcc
     6601 aagtcacctt gttttaactg gaaaacaagg aacaggtaaa agtcacctag cgatggcagc
     6661 ggcttgggaa gtcttgaagc gatcaaacta cgataaaaaa atcttattta tcgggttaca
     6721 ggaaatgctg gatcaaatca aattttcgta caacaatcct gaactcagaa aaacgattga
     6781 gggatcgttg attgcagata tcaaaacagc ggatttagtc atcattgatg atatcggttc
     6841 agaactagga aaagatgcat cagatagtcg agcgtttggc ataaacacgc taaattcgtt
     6901 cttggatgca cgacagaacc tagcaacgat tatcacaacg aacttgcttg gtgaagaact
     6961 gaaaaaagct tacggtacga gaacaatatc aagaatgttt gtcaactctg atggatttac
     7021 gatggtattt tctcaaacag cagacaagcg cataaaacca gtcaaaggag cgatcgcatg
     7081 aatttgacta aacaagaaaa aagaatctgt cgtcagataa tgttgcaaat tataggtgat
     7141 cgtgacatgg acgaattatc tcaacaagag agtcgaatgg ttcggaagat aagtaagcta
     7201 atcggtgcag atacgatgga tcgatccaag ccattaccca aactgaattt tgaatcattt
     7261 acgtttgaga aatatagcca tctgagagag gtaggctata gtgtaaatgc tattcgaaaa
     7321 gcacttgggg taggaagtag tgtatgggca aaatggcgga aagaaaatgt aaatgaggta
     7381 aatcaaaatg attaagctaa taggcaaaga gacgctcaag gtttactatg tagcggaaac
     7441 aaaggcagaa gtcagccgtt ggctattgaa aatgtttaat aagactagag ccaatgcttc
     7501 agtaagtcag aaagcaagtg tatcagaaac aggcatgcct gaagcaatgt ggatttcaag
     7561 gaggaactga gatggcaatg ataccgaagt ttagagcgtg ggatagagaa aaagacagaa
     7621 tgaaggcagt agctttggtt gaatttgctg aggcgtataa atcaggagaa ctcatttcac
     7681 gtacaaaata aaaaaagtcg gattcctccg actatgaata atatttccga cataagtatt
     7741 atatcataat tgggggaatc agaggatggt acttttcgat gtaaagaaat atgagacacc
     7801 aagcgcgaaa gatgttgata tggaacgcac aaaacataat gtcgctgtgt ttctttcagc
     7861 atatctatca gctagatgta gagtagggca acctcgagaa ccaaaagtga cagcatctta
     7921 ttccttggtt ccaccttcta cagctgatca tatatttgaa gcagaaagaa tgctgatcga
     7981 taaagaagaa gcacaggaag aatttgagta tctgcataaa ttgtttgttc gaggctattc
     8041 agcgatacag catccgcata agcctgatgt gactgaaagg cgcaagaaga tattctatga
     8101 tcgttatatc aatggtctgc ccatttatgt aactgctcaa aggaataata ctagcgaaga
     8161 atcggttaaa gtagaatcaa acagaattat catccaattt gcttcatcgt tagaactggt
     8221 tgctttcaag tagccagttt ttacactttt tatacccttt tattaccaat ttggtttcca
     8281 ttttatacct tttttatacc aatcacttac ctattcaacg ttgtattatg atagtgtcga
     8341 aagattagga aacaggatcg acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt
     8401 aattaagctt cgataggcag cagcggaaat attaagaata aggatgtgaa ttttaactcc
     8461 ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttattat gtcactgtgg
     8521 cggaaagggt agacgcttaa aaataaggtc aatacgtcga gggatagcct taacgtttta
     8581 tgatttgacc atgcaaggtt cgattccttg ccagcgactt agacgaaagg tagcttaaat
     8641 attaaccaca tcaacatcgt cagcgagttg ataaaggtaa ctagcagact ctaggattta
     8701 agccagtctt accgattagt ctgttatagt agcaagcttg ctagaggtag ctcctctggt
     8761 tgacgtgcag ctcagttggt agagcgcctg acttttaatc aggaggtcgc cggttcgaat
     8821 ccggtcacgt taataaagaa cctacggaaa caatcaatct tatcggatgc cgattggttg
     8881 gctgaattaa gcagtgtgtc tgtgaatcag ttaataggca taaaatacta gcacagacgt
     8941 gtgccactct caggtgtagg ttaggagaga aacattagca accgagggtt ggaaatgggc
     9001 gctcaaagta cacgagcaag gcgaggtcga tagtaatcga tggaatcggt gtaggttgct
     9061 attacataat tgattgggtt agattgagtt ttgggattcg gtacaaatga atcgtcaaat
     9121 gactcaagca caggatcgga aacgtccctg cctgtgcatt acatattaga tcactcgttg
     9181 agtggtcttt ttattttaga aaagagagga ttttgaaaat gaatcatgag aagtttatcg
     9241 aaaaatgcaa ggctattgta cgtgaaagaa ttgagaatga gattgctgac ctaagtggag
     9301 cagtacctga gtttagtgtt ttcatagtct ggtcatgcaa aacactacag aatagcaaag
     9361 cattagtcag cgctagctta aaaggagcac cgtattttga aattacgttg aacggggaca
     9421 aaggtgaaat ctatgtagat acttatctca aaaaatcaaa tgaatgtatc aaagtctagc
     9481 aggtgctagg ctttttcttt acataaagga ggaatgatct catgaaacaa tataccgcta
     9541 aagatttcga ggaaatgaag cgattaaaga aggactatga agaagttgat atggagctaa
     9601 ctgttggagt cattcaacga agactgcggg tcggattaga gacagcaaag gctatttaca
     9661 atgatctaaa tgctattgaa gagaagaatg gctaatgagg aactactggt atatatcgct
     9721 aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc gttctgtaca
     9781 aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat gtgagattga
     9841 tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca ttcaaaataa
     9901 tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct ccaatcagga
     9961 caaatcaagt tagacaaagt ttccgattta aagatattaa ttgaagcaga tctaatgttg
    10021 aaagatattg aaaattagaa aacaaaactc aacctaagaa gattgcgagg tggtgtgtat
    10081 tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg gttagaatcc
    10141 aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga ttctcaggtt
    10201 agaaaatgga aatcgcaaga caaatggagc gctgaattga aaagtaacgt taccaatggc
    10261 aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc taaaggtaac
    10321 aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa aacaggcgag
    10381 tatgaaacca tattttttga gacactaagt gatgaagaga aggacatcta ttctagtctg
    10441 aatgataatc cttcttttgt tttgtctgaa gaaatacgtc tacttaagat aagacaattt
    10501 cgtatgatga agagaatcca acaagctgaa gctggactaa atgatgaaga agtcgaacga
    10561 ttgcagcagc taagaaagat taaaaatccg attgaaaaaa atggtaaaaa gctagaaatc
    10621 aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa aattgatgat
    10681 attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa agccatcaag
    10741 caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc tcagactgat
    10801 aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga aatagaagaa
    10861 tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa tgaataatga
    10921 atttattcct tttgctgata ttggttctgc cattgattat tactatgata aaccagtagc
    10981 tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg ttttaaatga
    11041 tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa aaacagcatt
    11101 agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag taatagcaac
    11161 agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta aatggttaaa
    11221 tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata tggttggtga
    11281 ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaaata tgcaaggttt
    11341 tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg atccaattat
    11401 ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt gtggaaaccc
    11461 caataatatt gaaggtgttt tttacgattc ccacaattca gaccgtgata aatacagagt
    11521 tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag aaatgattct
    11581 taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag aatttcccaa
    11641 aggtgcgttg gattcattta tcagtcttga aacggttgaa ttggctacag aaaaacaaat
    11701 tagtgattct ttagtcaata aaacaacggt tgctcatatt ggtgttgacg tagctcgata
    11761 tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg agtatgagaa
    11821 gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg ccaagaatct
    11881 aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca ctggtgtcgg
    11941 aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc cttttgaggt
    12001 gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt taggtactca
    12061 actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc ttaacggaga
    12121 acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa gtactcgcaa
    12181 attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata tgaaaaagcg
    12241 aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg agccaccaag
    12301 tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt attatcttta
    12361 gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg aatgtttgat
    12421 ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag aaaatatcta
    12481 gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac tgatattgta
    12541 aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca agcagtcaat
    12601 cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa aagaacactc
    12661 attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac agttccagaa
    12721 gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct tgattctgaa
    12781 actgcaactt ttcaaggagc ttgtggagta gctttccgat tgttagagcc tgtggaagaa
    12841 gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga aagatatgga
    12901 aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt ttttcaagaa
    12961 atgaaagtta tgacaagaaa taaaattctg acatatgctt gctatggtga tttgaatttt
    13021 gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg gacgattacc
    13081 ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc tgaggaaatt
    13141 ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca atttaagctt
    13201 gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga aatgatgaat
    13261 cagctaggga ttatcaactt aaaagaccct actgcaaaag ctgaatatgt cacaaaaaat
    13321 ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt ttacaccatt
    13381 tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg agaagctcgc
    13441 aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata ttttagagaa
    13501 ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga caaagtagaa
    13561 attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga tttaagttat
    13621 ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat tcagagccaa
    13681 attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga aagtggaagt
    13741 gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga tagaaacgaa
    13801 gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag aaaatcagac
    13861 acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag caaaactatt
    13921 atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt atagtacaag
    13981 taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa aaatgtatga
    14041 tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg caaaagtcaa
    14101 tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg cgatggatgc
    14161 agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa catattcttc
    14221 taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt taccagaaaa
    14281 tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa gaagactttg
    14341 gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa gtgttgctag
    14401 tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa tttgtgacat
    14461 gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc gaacggagta
    14521 tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta ttgaggaata
    14581 caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa aggcaagtaa
    14641 gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt tccatagtcg
    14701 ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg atgaagaata
    14761 tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg gaattaattt
    14821 gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt aatgtaaatg
    14881 ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct aatggcttga
    14941 gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact gttaattatc
    15001 aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta ttatctcatt
    15061 cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt attgttgtta
    15121 gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt ccgaaatgac
    15181 gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt aaaactcaaa
    15241 gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg aaacacaaat
    15301 cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag ttttcttttg
    15361 atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt cagtcacaat
    15421 ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta gataaaatta
    15481 aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct gagcaaattc
    15541 aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata
    15601 ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag ttaaaagagt
    15661 ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc actaagaatg
    15721 ctgttgaagg ttttacaagt gtcttgaatg caattaatga gaaacatgca gatgcattaa
    15781 aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac caggttcagc
    15841 agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt caacaattta
    15901 actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt atttggatat
    15961 ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct atttatttgg
    16021 tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat atgatatcaa
    16081 caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg aatcaggtcg
    16141 aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat ctggtacatt
    16201 agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag cactacgtgg
    16261 tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa
    16321 tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag caaataaggc
    16381 agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat ttaaattgat
    16441 gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata tgcaggagtt
    16501 aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac
    16561 ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg ctctactaga
    16621 accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga aactacgtcc
    16681 attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac gtatcggtct
    16741 tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt taaatttaaa
    16801 gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca gtggtaaaaa
    16861 aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact aaaaattttg
    16921 ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat aacgaattta
    16981 tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt gatgttaatg
    17041 aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg agtctacaaa
    17101 agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa taattttgct
    17161 agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt
    17221 gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag tatcttcagt
    17281 tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta gtgagttact
    17341 tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg agttttttta
    17401 atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat tgaaagactg
    17461 acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa agtatatgat
    17521 aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt catagaagat
    17581 ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc taagattaaa
    17641 ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat tttttctcta
    17701 tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat aggaagtgag
    17761 aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga attttaaaaa
    17821 tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa tgaataaagt
    17881 cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc gaggatatcg
    17941 tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta taaacaatga
    18001 acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct ttgtcaaagg
    18061 gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc ctcgaagatt
    18121 taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat gaaatcattg
    18181 ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat cttgattcag
    18241 ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct ggaactgaaa
    18301 atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg attgataata
    18361 aacctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga
    18421 ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg
    18481 ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa
    18541 tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt
    18601 tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg ggaacagcag
    18661 taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc
    18721 aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc
    18781 ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg
    18841 gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat
    18901 accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acatcaacga
    18961 ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac
    19021 caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga
    19081 tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta
    19141 cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagatg aagaaggtaa
    19201 atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc
    19261 tgcaggagtt atggaagaaa tgcaaacagc gactgataaa caatcagtta aaaaagcttt
    19321 aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga
    19381 ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc
    19441 tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc
    19501 aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta
    19561 ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt
    19621 ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc
    19681 actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag atgatactag
    19741 atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg
    19801 aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt tggaaaatat
    19861 ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa
    19921 tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa
    19981 atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc
    20041 ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc
    20101 aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa
    20161 atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat
    20221 tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg
    20281 ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc
    20341 tgaaagaagg tttgtctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag
    20401 aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta
    20461 ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg
    20521 aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggaa
    20581 ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct gcaaatattc
    20641 ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt ggttctgctt
    20701 tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg ttcatgtttc
    20761 aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat gccataattg
    20821 gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaattca tttgttttaa
    20881 ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca accaatgaag
    20941 agtttagaaa caaggttata tctatttgga atgatgtgaa agattccgta attggtgtat
    21001 taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa atggcatcaa
    21061 atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt tctaacacct
    21121 ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag acttcaagaa
    21181 atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt tctgatactt
    21241 ggcaaagtat aaaagattca gccagagaaa aatggaatga aattaaaggt tctattatgt
    21301 cggttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt actttttatc
    21361 tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag attttaaaaa
    21421 atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa
    21481 caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata aatatttggg
    21541 aatctataaa gaatatattt gta
//
LOCUS       DAJSVP010000040        20849 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSVP010000040 DAJSVP010000000
VERSION     DAJSVP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.040"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            591..1280
                     /locus_tag="ON449_001526"
     CDS             591..1280
                     /locus_tag="ON449_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934114.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ON449_001527"
     CDS             1267..2475
                     /locus_tag="ON449_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3934115.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ON449_001528"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ON449_001528"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3934116.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON449_001529"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON449_001529"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3934117.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ON449_001530"
     CDS             complement(6578..7273)
                     /locus_tag="ON449_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3934118.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON449_001531"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON449_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3934119.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ON449_001532"
     CDS             complement(8472..8663)
                     /locus_tag="ON449_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3934120.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ON449_001533"
     CDS             complement(8684..9574)
                     /locus_tag="ON449_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3934121.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ON449_001534"
     CDS             complement(9561..10328)
                     /locus_tag="ON449_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3934122.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON449_001535"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON449_001535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3934123.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON449_001536"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON449_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3934124.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON449_001537"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON449_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3934125.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ON449_001538"
     CDS             14899..15282
                     /locus_tag="ON449_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934126.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ON449_001539"
     CDS             complement(15450..16208)
                     /locus_tag="ON449_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3934127.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ON449_001540"
     CDS             complement(16226..17044)
                     /locus_tag="ON449_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3934128.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON449_001541"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON449_001541"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3934129.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON449_001542"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON449_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3934130.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ON449_001543"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ON449_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3934131.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ON449_001544"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ON449_001544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3934132.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5949 a         4252 c         3827 g         6821 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatg
//
LOCUS       DAJSVP010000041        20782 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSVP010000041 DAJSVP010000000
VERSION     DAJSVP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.041"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            169..633
                     /locus_tag="ON449_001545"
     CDS             169..633
                     /locus_tag="ON449_001545"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3934133.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(695..1738)
                     /locus_tag="ON449_001546"
     CDS             complement(695..1738)
                     /locus_tag="ON449_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3934134.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(2115..3080)
                     /locus_tag="ON449_001547"
     CDS             complement(2115..3080)
                     /locus_tag="ON449_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3934135.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            3314..4159
                     /locus_tag="ON449_001548"
     CDS             3314..4159
                     /locus_tag="ON449_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3934136.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            4350..5537
                     /locus_tag="ON449_001549"
     CDS             4350..5537
                     /locus_tag="ON449_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3934137.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            5543..5899
                     /locus_tag="ON449_001550"
     CDS             5543..5899
                     /locus_tag="ON449_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3934138.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5901..6239
                     /locus_tag="ON449_001551"
     CDS             5901..6239
                     /locus_tag="ON449_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3934139.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    6315..6571
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6634..7668
                     /locus_tag="ON449_001552"
     CDS             6634..7668
                     /locus_tag="ON449_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3934140.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7661..8347
                     /locus_tag="ON449_001553"
     CDS             7661..8347
                     /locus_tag="ON449_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934141.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            8371..9219
                     /locus_tag="ON449_001554"
     CDS             8371..9219
                     /locus_tag="ON449_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3934142.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            9416..10189
                     /gene="sufC"
                     /locus_tag="ON449_001555"
     CDS             9416..10189
                     /gene="sufC"
                     /locus_tag="ON449_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3934143.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            10202..11488
                     /gene="sufD"
                     /locus_tag="ON449_001556"
     CDS             10202..11488
                     /gene="sufD"
                     /locus_tag="ON449_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3934144.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            11485..12720
                     /locus_tag="ON449_001557"
     CDS             11485..12720
                     /locus_tag="ON449_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3934145.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12707..13177
                     /locus_tag="ON449_001558"
     CDS             12707..13177
                     /locus_tag="ON449_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3934146.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            13182..14573
                     /gene="sufB"
                     /locus_tag="ON449_001559"
     CDS             13182..14573
                     /gene="sufB"
                     /locus_tag="ON449_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3934147.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14668..15810)
                     /locus_tag="ON449_001560"
     CDS             complement(14668..15810)
                     /locus_tag="ON449_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3934148.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(15849..16583)
                     /locus_tag="ON449_001561"
     CDS             complement(15849..16583)
                     /locus_tag="ON449_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934149.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            16815..17090
                     /locus_tag="ON449_001562"
     CDS             16815..17090
                     /locus_tag="ON449_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934150.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17188..19185
                     /locus_tag="ON449_001563"
     CDS             17188..19185
                     /locus_tag="ON449_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934151.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(19261..19596)
                     /locus_tag="ON449_001564"
     CDS             complement(19261..19596)
                     /locus_tag="ON449_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934152.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            19723..19938
                     /locus_tag="ON449_001565"
     CDS             19723..19938
                     /locus_tag="ON449_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934153.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19975..20337
                     /locus_tag="ON449_001566"
     CDS             19975..20337
                     /locus_tag="ON449_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3934154.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            20383..20709
                     /locus_tag="ON449_001567"
     CDS             20383..20709
                     /locus_tag="ON449_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934155.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6898 a         3629 c         4318 g         5937 t
ORIGIN      
        1 atttgatttt acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca
       61 tgtttcctcg gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg
      121 atagagaaaa aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac
      181 acacctatcg gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt
      241 cagccaattt cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag
      301 tttgaagaat attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga
      361 ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc
      421 cgaacgtata aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg
      481 caggcaaatc gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa
      541 aatggaaaac tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa
      601 ttgttgttgt tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc
      661 atgcgagaat cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata
      721 gtaaagccat cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac
      781 cttctttcat gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct
      841 caaaccagct gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc
      901 tgttgaccaa acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt
      961 ttttctgcgt atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt
     1021 caaacagata gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga
     1081 attcgacaac agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga
     1141 gtgtaccacc caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa
     1201 gaaaagggag agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc
     1261 ctttttcttc ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac
     1321 aaacagcgtt tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac
     1381 caatcaagta agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt
     1441 ttacaccaat ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga
     1501 attctaatag tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa
     1561 aagtatttcc taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca
     1621 gataaatagc aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta
     1681 agatttcgcg gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt
     1741 gatcggttcc tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca
     1801 gtattttgac aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa
     1861 taaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
     1921 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct
     1981 tggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat
     2041 gaacagctgt ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc
     2101 tattgaatcg attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct
     2161 ttaattaaga aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga
     2221 cattccgcaa cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa
     2281 tgaacagtag taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag
     2341 cttggataaa aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat
     2401 acttgagata aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta
     2461 agataacctg ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc
     2521 ggatgagcca tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca
     2581 tagcagatca atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca
     2641 gttacagtga tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca
     2701 attgaaagat cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg
     2761 aagataacag cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca
     2821 aaataagtac aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt
     2881 ggatcatgca acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat
     2941 atcataagaa tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct
     3001 ttaaacagat ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata
     3061 attttcaaaa agttcttcat atataatatt ctccttttta tttaatagat tttagtacca
     3121 agatgaacga atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct
     3181 gattgtcttc atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta
     3241 tagatggttt tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta
     3301 tggaagtggt gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa
     3361 tttttccaga gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa
     3421 acagttggag cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct
     3481 tccaactaag caagccgact tattatatga acggacactg gttttgttag aagaatggga
     3541 agaaactcag caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc
     3601 ttcccacacc tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt
     3661 tcctaaagtg cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc
     3721 gaaacatgaa tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag
     3781 atactctttg atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct
     3841 tgtaagagaa gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa
     3901 tatccaagat aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga
     3961 aggaatcggt cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc
     4021 gttgaatttc catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct
     4081 gctagagatt gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga
     4141 ggagaatctc agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag
     4201 ataagcagta ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt
     4261 tttcccaaat gtctatggaa agactttcca ttatctaact ggaccgctat aataatcagc
     4321 agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata
     4381 atcgtattga ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct
     4441 cattatatgt agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga
     4501 tgcaagttgt ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt
     4561 ccaaatggat ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac
     4621 tcttgctcaa attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc
     4681 gattcggtgc gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga
     4741 tggcactgat cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact
     4801 tttggctgat tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc
     4861 aagatgactt tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt
     4921 ctggaatctc ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag
     4981 gaacgatctt cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca
     5041 aatcttatca gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa
     5101 tgagttatca gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag
     5161 gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta
     5221 tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa
     5281 tctatcgtat gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa
     5341 gcggtttgat catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat
     5401 tacttccttt aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag
     5461 ggaacatgat cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa
     5521 gatcttcaag gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg
     5581 caaaaaagcc aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat
     5641 caaagaagta ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat
     5701 ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt
     5761 aggcgagttt acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa
     5821 gcgaccactg atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta
     5881 tgaaggagaa ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga
     5941 tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg
     6001 gagacatcac gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc
     6061 tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag
     6121 tttcttctgt taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa
     6181 tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa
     6241 atcagaaaat cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca
     6301 atcctatatg aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag
     6361 agccctgttt gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt
     6421 aagcgagcgt aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa
     6481 gaagctaagt ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg
     6541 taacacggaa gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg
     6601 tttgaaaaat cataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc
     6661 aaaaagacat tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac
     6721 gttgaaaaag gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta
     6781 gtccgtctcc tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa
     6841 gatattacaa aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc
     6901 ttccagcatt tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg
     6961 gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta
     7021 gtcgggttag aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa
     7081 cgcgtcggta tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct
     7141 actagcgcat tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat
     7201 aagaaattga atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc
     7261 tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact
     7321 gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca
     7381 gaagctgatt cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg
     7441 gcaactttga cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga
     7501 cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc
     7561 tttggttcat tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg
     7621 ttcatcaaag aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga
     7681 aacatatcta gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac
     7741 actatatatg acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt
     7801 gatcctttac tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt
     7861 atcgatcatc agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat
     7921 tccatttact aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct
     7981 tgttttatcc gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag
     8041 ccctggtgta ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa
     8101 ggtcttgata cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc
     8161 gatgatcgga tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc
     8221 ttggcaggaa ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat
     8281 tttactaatc gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa
     8341 aagataatag gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca
     8401 gcgcttttgc tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca
     8461 gcaaataaag cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag
     8521 attttggaac atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa
     8581 ttcgatgact atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac
     8641 ttccagcata ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat
     8701 gccggtgctg tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc
     8761 gaattaaaag atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc
     8821 atcacaattc tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca
     8881 gcaacattcg atgatattga agaaaaccct aagaacttga agttcaacca tacgatcgac
     8941 ccatcacttt tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca
     9001 aacttcgctt acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac
     9061 aactctcctt acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc
     9121 aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg
     9181 ggcggctctg ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga
     9241 gaaataagtc tgtaatggct tatttctcat cttttttata cctattctaa tttagaaatc
     9301 aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc
     9361 attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc
     9421 tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt
     9481 aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa
     9541 atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat
     9601 tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt
     9661 atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg
     9721 tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa
     9781 aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct
     9841 gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt
     9901 agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa
     9961 agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac
    10021 ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg
    10081 ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc
    10141 tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag
    10201 aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa
    10261 aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact
    10321 gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga
    10381 aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc
    10441 tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga
    10501 aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca
    10561 agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt
    10621 agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc
    10681 actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt
    10741 gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga
    10801 acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa
    10861 aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg
    10921 tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca
    10981 tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat
    11041 cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc
    11101 ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac
    11161 ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag
    11221 tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga
    11281 tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat
    11341 gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg
    11401 attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga
    11461 agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct
    11521 atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc
    11581 caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat
    11641 gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga
    11701 gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt
    11761 acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga
    11821 gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg
    11881 gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat
    11941 atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta
    12001 tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac
    12061 ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa
    12121 gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt
    12181 ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc
    12241 gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc
    12301 gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg
    12361 caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg
    12421 ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg
    12481 acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta
    12541 gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac
    12601 cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc
    12661 gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa
    12721 attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg
    12781 aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat
    12841 cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg
    12901 ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat
    12961 agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa
    13021 agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg
    13081 gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac
    13141 cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag
    13201 aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct
    13261 tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg
    13321 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
    13381 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
    13441 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
    13501 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
    13561 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
    13621 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
    13681 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
    13741 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
    13801 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
    13861 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
    13921 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
    13981 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
    14041 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
    14101 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
    14161 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
    14221 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
    14281 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
    14341 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
    14401 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
    14461 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
    14521 cagtcgaact caatcgattg atcagttatg agatggaagg cagtgttggg taaacctgtt
    14581 ataggagcta gaacccttga tatataaggg ttctagcttt tttgttttgc ctgtatacta
    14641 gaaatgacca gattttgacc agctctttta ctgatgagcc atcatatcag caaagtcttg
    14701 acctgttttc ttaggtgcat tatttgtaat atgtgcatag atgtctaatg tcactttact
    14761 attttcatgc cctaatcgtt gttgaactac cttgacagat tctcctgctt ctaataataa
    14821 actagcatgt gtatgtctaa acccatgagg tgtaatacga ggcaagttat attttttcaa
    14881 aatatatctt aaccaatcat taacagtgtt tggaatgtat agagtgtttt taactgttgt
    14941 aaaaacatgt tgctctttag agctagtatt gtatccaagt tttaaatact cttctttttg
    15001 ctgtaatttc cagttgttga ggatagaaag agtctcagta tctaaagaga taactcgtct
    15061 agaagctctt gttttcggag attgaattat aactttacca aattcatcta cagcaagagt
    15121 tttattgaca tgtagttctt tattaaacgt gtctatgtct ttccactgta aagcgagtac
    15181 ttcactcttt ctcatacctg tgtatgccaa taatctgaag aaggcgtatt gtttcatgtt
    15241 accaaaatct ttaaaagcat ccagtaaatt attaagctcc tcttttgagt aaaatttacg
    15301 atttttctca tactcttttt ttcgtggaag aagtgttttt tccataggat tattgtcaat
    15361 atattgcatt gcaaccccgt agcgtaaaat ttgtgcagtc tgttttctca tataatcgta
    15421 actttcatat ttactgtgcc attcatctac cactttttga caatatggga cagagatatt
    15481 cgatagttta agcttaccaa atgctggaag tatttgatta tttgcgtatc ttactgagat
    15541 tgcgattgtt gaagctttaa cactggtacg ctgatgagtt atccattcat tatacaggtc
    15601 ttcaaatgtc atatcaggtg ctttcttttt aacaccgtat aatatttgat gtctgtactc
    15661 gtcataatct ttttgagctt cttgtttgct actaaaacct cttctcgtag tatttatttt
    15721 ttcaccagtt cgtggatcgg tggcaatgtg tcctttatac atccacgcca cttgaccgtt
    15781 tttctttttg tactttgata tttttgccat taaatcatct cctcttagtt tacaaggagt
    15841 acttaccact agatggtatc acctcccttc atttttttct tagtgtattt tttcccaatt
    15901 gatgaaatcg tttgttctag cttgtttata ttatcagtaa tttctctcac agcttcttcg
    15961 ggataagaca tgtctttcaa ttgaataggt agccattcat ctaccattct ttctagtagt
    16021 tcttctatac tgctagtgag gaaaagaatg ttattgtcaa attcttctac aatagcattg
    16081 atgaataatt cttctatttt taagatattg aaaagactaa ttttttcatt tttaacttta
    16141 ttacaagtct tgttagcaat aaattcttgc atctctggtt ctaatttgga atatctatcc
    16201 agccatgtat ctaaattaga gtgtactggt gggaactgtt gacgaagatg ctggaacagg
    16261 tttttatctg gaaatgttat accatctagt acaatgctat ctctataaag aataattccc
    16321 aaaatatatt cttctacatt gccacaaact aactcctcta tactcatatt tcccaaatca
    16381 gcaattcggg ataatcgctc tggtattggt aaataggttc ccttctccca ttcgtagata
    16441 gtttttttgt caattaattg accgttgtta atcataagac caaatgctgg ttgtgttaat
    16501 tttttactct ttcttatagc ttttattttt gttccgagta tcttttttcg ttctaaataa
    16561 tttattggtg gacgttttat catattgacc acacctccta aaattagtat acctcaaaaa
    16621 aggggtgttg taaataaatt ttacaaagct gttgacaccc cttttttatc gaggtattat
    16681 gtgtatgtcg atgaactggt aatcattttt tgtttgatta atcggttaat aacaaaatac
    16741 ttttattttg agcaaaaaat acgaagataa ttcttagaaa atttgttcaa gcaatcacga
    16801 aaggaggggg aaatatgtct ctacaaatca ttgatagcat tgtttctata caagttgaac
    16861 aacaagtgaa agagataatt acagtgctaa agcctatgct agaagacatt atttctggag
    16921 tcactctaga gtatatggac tatcagcagg caagtgaata tctaggtgta tcagtaggaa
    16981 cgattagaaa atatgtttct caatacgggt tacctgttat aaaaattgat acggtcatta
    17041 gatttaaaaa atctgatatt gatagtttca tggaacggta taaaggttga gtaaacaaat
    17101 aagactaaaa catatagtaa taaagagttt gtttttatta tctgtatgtt ttagtcttta
    17161 acagaatttt ataaggagga aacacaaatg gaaacatcaa tgattaatat tccaatttat
    17221 gtagatcaag aaaaattatt agagaagcca gttacaaaaa aagaaaaaac tgatgtaaga
    17281 gataggtgtt tagataacta tagagaagtt acaagagatg agtttgtaga gatactaaat
    17341 tcatcacaat catttattcc aagcaagaca aagaataaag gcaatgacaa ggagagtttt
    17401 gtggaaacca gagtaattat tctggatgtc gataacactg ttaaagatga gaaggggaag
    17461 gtagtagatt tgagtaaaga tgatagtaga tacttaagca tagaaaaggt tttatctatc
    17521 aatttagttc ataattctgc gtttgcgata caaaaaagta ttcggtactc tgaaaatcta
    17581 gaaaaataca agattgtctt tctattaaaa gaagcaatta ctgactatgg taaaatggtt
    17641 gctgtttacg aatacttgaa aaagaacatc cccgggtgtg atgacaatgt aaatgcttcg
    17701 aatagaatgt tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac
    17761 atgttagata ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa
    17821 agtgattcag ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa
    17881 aatgacgaca aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac
    17941 atgtctttta atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag
    18001 tcaagtaaaa acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt
    18061 accagtaaaa ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa
    18121 aattttattg gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat
    18181 tggctattag agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca
    18241 aaagtacttt ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat
    18301 tatctagagg aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat
    18361 gatagtaagg aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt
    18421 tcttactatg gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact
    18481 tttataggat tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac
    18541 ctaaataggt taaaggaaga gaaagcgtta ggaaacttta atcataagag aagcaatgtc
    18601 tatcgattag acgtttttaa tgcaacttct gtgattcaaa aattgaatga agatatcctt
    18661 ccacttctgg aaaaaattca ttttacatat caatattttt cttatgaatg ggttaagata
    18721 tgttttaacg aaaaagaggc taaaagagta tttcctcaaa attcccgatc agaactcagc
    18781 aaggaaaaac aattgatttt agatgaagca catgaagttt tacagggctt atcgtggcaa
    18841 gggacatatc taatgacaga gaaagaactt attaacactg tttgtgaacg cctacgtaat
    18901 gttggggata acagcgtgaa aaatgcttta ataaaaaaca gaggctactt tgttaaacaa
    18961 ggattcactc ttgaacgagc ttctaaggat attaagaaac tatacaatgt gaaagcaagt
    19021 agtagttttt taatatattt agcgaaaggg gcagggatag atgaaacaat aaaagaattg
    19081 atattatcag gagctgggat agttcctctg tatcaattta caagtgtaac aagagatgat
    19141 ggacggagag taaggaagag aaaagaccca attgtattaa actagacagg ttatccatag
    19201 ctcttaatct tatttcatct caatacaccc accgttaaag ttcggtgggt tatttatttt
    19261 tcaatctttc aatagctgat tgcgttcatt ttcttgcctt tttaatagct tcatatagtc
    19321 aattgttcga actgcatctt cagtgctaag agaacgatag agagccaaaa actcttcctc
    19381 ataatctacg acaacattgt ctagaaaatg gcttacaggg gttcctaggg ctctagaaag
    19441 agctgttaag ctatcgaggt taggctctgt tacagaattt tcccatcttg ctactgcctg
    19501 ttgtgtagag aagatttttt cagctaattc tttttgtgtc cacccgagag cttttcgctt
    19561 ttctctaatg acctttgcat attgtttctg tttcattaat acacccccat ctcagtatag
    19621 tacaacaagt tgttgtattt gtaatggtat ttgaagtttt ttatatttta tgagttgaaa
    19681 tacatcatat tgttgtatat aataaaacag gaggggagaa tcatgaaaaa agtaagtaat
    19741 ctcagccgat acagaaaaga aaaaggactt tctcaaacgg agttagcaaa aaaaatgaat
    19801 gttacacagc aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa accctatcaa
    19861 atgaagatgc tttctgagat attaagtgta ccaatgaatg agctgttttc tgatgtattt
    19921 aataaagtaa acagttaact aataagcaat tgaatgattt taggaggaga agacatgtat
    19981 tgtagaaaat gtggcaaaga aataacagat gaaagtaagt attgccagtt ttgtggagca
    20041 ctacaagaaa cagatgagac aaccggaaca gacacagtaa agaaaagtac tgagcagaca
    20101 attaatgaaa tgaaggaaca aatcgtacat ggacaagaga cagggaagct gtatttgatt
    20161 attggttgga tttctatggt agtatcgtta gcatttattc ctgttgtatt tggtgctgtt
    20221 gctgttatta tgggctactt atatcgagaa aaagatgaga aaatgggtac gattttgatg
    20281 atagcagggg tagctggagc aatctttggt gtacttttag ggatgtcaat gtactgataa
    20341 gagggataag aacaaattat tttggagata ggagataaaa agttgaataa agtaaatatg
    20401 aacaagaagt ttgtcattat aggtattgtt gtcttggcta ttataggtat tattttttca
    20461 aaaatgacag gtacatctgg attagagaag tcatcaaaac cattggtaac acagatttta
    20521 caagaacagt acaacttaaa tcgatcatgt gatgatgtga cgattactca tgaaaatgga
    20581 gataacacat accgggctaa agctatatta gacaatggaa gtgctatcaa cataaatatt
    20641 gagtactatc caaagaaaga ccgtatatat gtggagatac catatgctga ggtgttaatg
    20701 ttaaattgat ttaaatgtat tatgcatacc gtccaaacag gaactcccaa aggtgtaaat
    20761 tcactaggga gtttttatag tt
//
LOCUS       DAJSVP010000042        20334 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSVP010000042 DAJSVP010000000
VERSION     DAJSVP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.042"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            66..1490
                     /locus_tag="ON449_001568"
     CDS             66..1490
                     /locus_tag="ON449_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3934156.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1789..2496
                     /locus_tag="ON449_001569"
     CDS             1789..2496
                     /locus_tag="ON449_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3934157.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2483..2806
                     /locus_tag="ON449_001570"
     CDS             2483..2806
                     /locus_tag="ON449_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3934158.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2895..3932)
                     /locus_tag="ON449_001571"
     CDS             complement(2895..3932)
                     /locus_tag="ON449_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3934159.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4253..5236)
                     /locus_tag="ON449_001572"
     CDS             complement(4253..5236)
                     /locus_tag="ON449_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3934160.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5651..6421)
                     /locus_tag="ON449_001573"
     CDS             complement(5651..6421)
                     /locus_tag="ON449_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934161.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6414..7349)
                     /locus_tag="ON449_001574"
     CDS             complement(6414..7349)
                     /locus_tag="ON449_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934162.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7665..9314)
                     /locus_tag="ON449_001575"
     CDS             complement(7665..9314)
                     /locus_tag="ON449_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3934163.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9431..12034)
                     /locus_tag="ON449_001576"
     CDS             complement(9431..12034)
                     /locus_tag="ON449_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3934164.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12046..13095)
                     /locus_tag="ON449_001577"
     CDS             complement(12046..13095)
                     /locus_tag="ON449_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR3934165.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13121..13435)
                     /locus_tag="ON449_001578"
     CDS             complement(13121..13435)
                     /locus_tag="ON449_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR3934166.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13451..13897)
                     /locus_tag="ON449_001579"
     CDS             complement(13451..13897)
                     /locus_tag="ON449_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3934167.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13898..15820)
                     /locus_tag="ON449_001580"
     CDS             complement(13898..15820)
                     /locus_tag="ON449_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3934168.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16032..16928)
                     /locus_tag="ON449_001581"
     CDS             complement(16032..16928)
                     /locus_tag="ON449_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3934169.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(16921..17601)
                     /locus_tag="ON449_001582"
     CDS             complement(16921..17601)
                     /locus_tag="ON449_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934170.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(17602..18384)
                     /locus_tag="ON449_001583"
     CDS             complement(17602..18384)
                     /locus_tag="ON449_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934171.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(18377..19294)
                     /locus_tag="ON449_001584"
     CDS             complement(18377..19294)
                     /locus_tag="ON449_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3934172.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(19458..19781)
                     /locus_tag="ON449_001585"
     CDS             complement(19458..19781)
                     /locus_tag="ON449_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934173.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6000 a         4196 c         3416 g         6722 t
ORIGIN      
        1 caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa
       61 aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt
      121 ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc
      181 gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg
      241 atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct
      301 ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc
      361 ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag
      421 tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg
      481 aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg
      541 gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt
      601 atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat
      661 tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta
      721 gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg
      781 atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg
      841 ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg
      901 aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac
      961 taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc
     1021 catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat
     1081 accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc
     1141 attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac
     1201 gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc
     1261 atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt
     1321 ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt
     1381 cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat
     1441 taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa
     1501 ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac
     1561 tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc
     1621 ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt
     1681 ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag
     1741 tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
     1801 ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt
     1861 ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg
     1921 atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc
     1981 catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg
     2041 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
     2101 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
     2161 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
     2221 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
     2281 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
     2341 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
     2401 gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc
     2461 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
     2521 ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga
     2581 tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
     2641 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
     2701 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
     2761 cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
     2821 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
     2881 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt
     2941 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
     3001 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
     3061 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
     3121 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
     3181 atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta
     3241 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
     3301 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
     3361 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
     3421 atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt
     3481 tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
     3541 ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
     3601 tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg
     3661 cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca
     3721 tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
     3781 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
     3841 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa
     3901 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
     3961 ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt
     4021 catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg
     4081 ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt
     4141 attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt
     4201 gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt
     4261 cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag
     4321 tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat
     4381 agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat
     4441 cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg
     4501 gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta
     4561 cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg
     4621 tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc
     4681 aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta
     4741 tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga
     4801 tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg
     4861 ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg
     4921 ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt
     4981 ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg
     5041 taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt
     5101 ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata
     5161 caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc
     5221 aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct
     5281 ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa
     5341 aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc
     5401 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat
     5461 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
     5521 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa
     5581 atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     5641 aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt
     5701 acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg
     5761 ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg actttcgaaa
     5821 cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta
     5881 acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc
     5941 cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca
     6001 tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc
     6061 cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag
     6121 gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg
     6181 aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga
     6241 attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa
     6301 taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca
     6361 aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca
     6421 tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga
     6481 ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt
     6541 ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta
     6601 caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt
     6661 tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat
     6721 tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa
     6781 atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat
     6841 tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat
     6901 gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt
     6961 ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg
     7021 cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga
     7081 ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta
     7141 tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg
     7201 attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct
     7261 tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc
     7321 aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct
     7381 ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt
     7441 tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt
     7501 ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt
     7561 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     7621 aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc
     7681 cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct
     7741 gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
     7801 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
     7861 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
     7921 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
     7981 tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
     8041 tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
     8101 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
     8161 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
     8221 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc
     8281 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
     8341 aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact
     8401 tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
     8461 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
     8521 atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct
     8581 cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc
     8641 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
     8701 gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag
     8761 attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga
     8821 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
     8881 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt
     8941 tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca
     9001 aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt
     9061 ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc
     9121 catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat
     9181 ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt
     9241 gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat
     9301 tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg
     9361 cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta
     9421 tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg
     9481 ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag
     9541 gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa
     9601 aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt
     9661 tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa
     9721 atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt
     9781 ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat
     9841 actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat
     9901 ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg
     9961 aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat
    10021 aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat
    10081 taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt
    10141 caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg
    10201 ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt
    10261 gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag
    10321 ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt
    10381 gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt
    10441 ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat
    10501 caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat
    10561 atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat
    10621 ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt
    10681 caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag
    10741 catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga
    10801 agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag
    10861 tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt
    10921 ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa
    10981 tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca
    11041 aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg
    11101 caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta
    11161 ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac
    11221 gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct acataagaag
    11281 gataatctga aataatattg tggaatttac tgtatttatt caacttctct acgacttgat
    11341 caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt
    11401 ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa
    11461 tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg
    11521 cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt
    11581 caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag
    11641 gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat
    11701 atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca
    11761 ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca
    11821 gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag
    11881 tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag
    11941 taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc
    12001 agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc
    12061 tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa
    12121 cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa
    12181 aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag
    12241 accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac
    12301 ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc
    12361 attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc
    12421 aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga
    12481 aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc
    12541 catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag
    12601 ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat
    12661 gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta gactaataac
    12721 ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag ctaatccagc
    12781 aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag tgataaaagc
    12841 tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa tcatcggtac
    12901 gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg tatccgtctg
    12961 gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc ctgctgctac
    13021 gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt ctcttccttg
    13081 tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta attaatgcaa
    13141 ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct actgttaaaa
    13201 tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca aatatgacaa
    13261 catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata gccccctgtg
    13321 tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta gcttttgcca
    13381 tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt gtcatatgaa
    13441 attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc gaatctttta
    13501 ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa atacgtaaat
    13561 gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca actggttgat
    13621 catctaatgc gccccattca acagaccgct ttaacttggc tacagccact gttgattcaa
    13681 ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct gtggtcatat
    13741 ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca aacccattag
    13801 tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca tttaaatcta
    13861 tcactatatt atttatattg attttttcac tgatcattta taagccttct ttcaatatat
    13921 aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca tgattcgaag
    13981 cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt tcattaagtg
    14041 caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct ggattaacgt
    14101 agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata gcaaaatcat
    14161 tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc ccttctttcg
    14221 cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact tgattatcct
    14281 ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa gcatcttctg
    14341 tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct aacatcaaag
    14401 gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt
    14461 catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct atctcaacca
    14521 tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca tatccgctac
    14581 tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt gattgaatat
    14641 ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc tcttcgattt
    14701 cctctaataa atctacactt atcataaatg accttttaaa agaatatttg attttgtccg
    14761 tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca ttgagatgta
    14821 atactagatt attgatcaat tcttcatcag gataaaattc atttaattta attcttacta
    14881 actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat
    14941 tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg
    15001 gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga
    15061 tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg
    15121 caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct
    15181 tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat
    15241 tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg
    15301 ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt
    15361 catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta
    15421 gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt
    15481 cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag
    15541 ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa
    15601 gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat
    15661 ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag
    15721 atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt
    15781 atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac
    15841 agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta
    15901 gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcctgcaat atttatatca
    15961 caggcttttt tctctattaa atatatataa gataatacat ttttctacta gttctcacta
    16021 atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc cccgtctaat
    16081 ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta aagcccgatt
    16141 ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg gaagactttt
    16201 tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg aatgaatgga
    16261 tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa attctgtatg
    16321 atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt atttatcgag
    16381 tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa agagaaaaat
    16441 acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc
    16501 tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc aacagattgt
    16561 ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc cagtaattgt
    16621 acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc gacgatcgat
    16681 agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt ttcatccaac
    16741 catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc gactgaaaga
    16801 cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa tctcttgatt
    16861 tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag caacagccat
    16921 ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat gtgcctttgc
    16981 tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat caaaatatgg
    17041 ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg gatgttctaa
    17101 aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac cattcacatt
    17161 tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc
    17221 ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca aaagttcttc
    17281 gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg aaactttatc
    17341 tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct gtcggatttc
    17401 tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga tcaaatcgac
    17461 aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa gaagtttgta
    17521 tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt cgataatcaa
    17581 aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa acattcctag
    17641 agctgatgat accacgattg ttactagact gaccagaatc attttcggtg cttgcgaata
    17701 agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc actccgttag
    17761 aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc ccatgatata
    17821 aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca aagccactgg
    17881 aacagataat gtccctaatg tcagcagaag caatacagga aatactttta gaacataatt
    17941 tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt aagcaagcat
    18001 ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt
    18061 cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa tcgtaccgtt
    18121 ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc
    18181 agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat ccgttgcttg
    18241 gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg
    18301 aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata cttttatttt
    18361 ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag aaataatctt
    18421 ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct accaatgctt
    18481 gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg ctagattgta
    18541 caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc gtcgccgaag
    18601 tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat aattctttga
    18661 tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat tcatctagca
    18721 agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg atgatttctc
    18781 gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt
    18841 ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct aaagagaaat
    18901 tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg tcttcgattt
    18961 ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt gcagttaatt
    19021 ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat
    19081 ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc ataatagttc
    19141 tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc attccacgtt
    19201 tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg gttataccag
    19261 ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct tattgatgag
    19321 tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa agtgtaaatt
    19381 ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat tatcaggcag
    19441 tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt cgtttctctg
    19501 tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa ttttaccaga
    19561 ccggttataa ggataactat agcaagactc cctcccagaa aaattagact gtctcgcaga
    19621 cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc aagtggactg
    19681 gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg cgttggaact
    19741 tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta aaaacggact
    19801 tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa gaggttatga
    19861 caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc aactcctcgg
    19921 tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc ttcttatttc
    19981 ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg tttgatgata
    20041 ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag aatcctacct
    20101 attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag
    20161 ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg
    20221 aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg ggctttctta
    20281 agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt tata
//
LOCUS       DAJSVP010000043        19990 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSVP010000043 DAJSVP010000000
VERSION     DAJSVP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.043"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            436..1545
                     /gene="pdhA"
                     /locus_tag="ON449_001586"
     CDS             436..1545
                     /gene="pdhA"
                     /locus_tag="ON449_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3934174.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1548..2525
                     /locus_tag="ON449_001587"
     CDS             1548..2525
                     /locus_tag="ON449_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3934175.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2550..4193
                     /locus_tag="ON449_001588"
     CDS             2550..4193
                     /locus_tag="ON449_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR3934176.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4201..5607
                     /gene="lpdA"
                     /locus_tag="ON449_001589"
     CDS             4201..5607
                     /gene="lpdA"
                     /locus_tag="ON449_001589"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3934177.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5769..6044
                     /locus_tag="ON449_001590"
     CDS             5769..6044
                     /locus_tag="ON449_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3934178.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6050..6817
                     /locus_tag="ON449_001591"
     CDS             6050..6817
                     /locus_tag="ON449_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3934179.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6985..8814
                     /gene="typA"
                     /locus_tag="ON449_001592"
     CDS             6985..8814
                     /gene="typA"
                     /locus_tag="ON449_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3934180.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9075..10847
                     /gene="recQ"
                     /locus_tag="ON449_001593"
     CDS             9075..10847
                     /gene="recQ"
                     /locus_tag="ON449_001593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3934181.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10961..11263
                     /locus_tag="ON449_001594"
     CDS             10961..11263
                     /locus_tag="ON449_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3934182.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11266..12429
                     /locus_tag="ON449_001595"
     CDS             11266..12429
                     /locus_tag="ON449_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3934183.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12455..15883
                     /locus_tag="ON449_001596"
     CDS             12455..15883
                     /locus_tag="ON449_001596"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3934184.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15904..17028
                     /locus_tag="ON449_001597"
     CDS             15904..17028
                     /locus_tag="ON449_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934185.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            17018..17455
                     /locus_tag="ON449_001598"
     CDS             17018..17455
                     /locus_tag="ON449_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3934186.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17439..17777
                     /locus_tag="ON449_001599"
     CDS             17439..17777
                     /locus_tag="ON449_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3934187.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17896..18456
                     /gene="rsmD"
                     /locus_tag="ON449_001600"
     CDS             17896..18456
                     /gene="rsmD"
                     /locus_tag="ON449_001600"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3934188.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18453..18944
                     /gene="coaD"
                     /locus_tag="ON449_001601"
     CDS             18453..18944
                     /gene="coaD"
                     /locus_tag="ON449_001601"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3934189.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18934..19977
                     /locus_tag="ON449_001602"
     CDS             18934..19977
                     /locus_tag="ON449_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3934190.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         6771 a         3490 c         4392 g         5337 t
ORIGIN      
        1 atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat
       61 ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata
      121 aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag
      181 tttgattaga aaatagaaaa aatgcagatg aaatataaaa cgctttcctt attgtgattt
      241 tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt
      301 gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag
      361 tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag
      421 aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat
      481 gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac
      541 aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta
      601 tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt
      661 tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa
      721 gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct
      781 ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa
      841 ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt
      901 gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc
      961 ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat
     1021 gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact
     1081 gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat
     1141 ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt
     1201 ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct
     1261 ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat
     1321 ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa
     1381 gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa
     1441 gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca
     1501 atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa
     1561 caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg
     1621 ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc
     1681 aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag
     1741 gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg
     1801 gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa
     1861 tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata
     1921 caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag
     1981 tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta
     2041 acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc
     2101 cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg
     2161 tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg
     2221 caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag
     2281 aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac
     2341 gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat
     2401 tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag
     2461 aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat
     2521 tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaattg ccggatatcg
     2581 gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca
     2641 atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa atcccgtcac
     2701 ctgttacagg aacggttaaa aatgtgattg ttccagaagg taccgtagca aatgttgggg
     2761 atgtattagt agaaatcgat gcaccaggac acgaagacaa tgaaggggac agtggtgtag
     2821 ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc cactgttgat acagaatcag
     2881 ctggatcgtc aagcgaagga gtcttccaat ttaagttacc ggatatcggt gaaggaatcg
     2941 cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata
     3001 cattactaga agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga
     3061 cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag
     3121 aaatcgatgc tcctggacac aatagtgcat cgtcaactag tgcgccatca gctgaagcac
     3181 caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata
     3241 aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct
     3301 cacaagtaac tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct
     3361 tggcaggtgg tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga
     3421 aagaagcagc tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca
     3481 acttaggaga cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag
     3541 caatggtcaa cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt
     3601 ctaaactatg ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac
     3661 ttactttctt gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat
     3721 tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg
     3781 gtattgcaac tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca
     3841 aaggaatgtt tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca
     3901 aactatctgc agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag
     3961 gtggcggttg gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa
     4021 cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga
     4081 aactgtcatt aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga
     4141 ataatatcaa acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa
     4201 atggtagttg gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt
     4261 ggctatgtag ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga
     4321 gagtatattg gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct
     4381 gcaggacacc attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac
     4441 gtcaaactag attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg
     4501 acttctggtg ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg
     4561 ttcttcgtag atgatcacac actacgcgtc attcatccag attcagctca aacttattcg
     4621 ttcaataacg caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc
     4681 ggaggacgtg tgttagattc tactggcgga ttagcattga aagaagtacc taagaaattt
     4741 gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga
     4801 gcagaagtaa cgattcttga aggctcacca caaattttac caacttacga aaaagatcta
     4861 gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg
     4921 gcgaaagaag cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa
     4981 gaagaatccg ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat
     5041 gatatgggct tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat
     5101 aaacaaggac gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg
     5161 gcattagcac acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa
     5221 aaagtagctg tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct
     5281 tctgtaggta tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa
     5341 ttcccatttt cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt
     5401 ctagttacga cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca
     5461 agtgatatgg tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc
     5521 gccttgacga ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta
     5581 gcattaggtt taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa
     5641 tcagttgcgt tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg
     5701 acaacttctt ggtttattac cacttcctat gctacactga agaggaatat gaaataggag
     5761 agttgaagat gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg
     5821 tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg
     5881 aagctttttt gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa
     5941 aacgattggg acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc
     6001 aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat
     6061 gattgatcat atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca
     6121 aacattaaca atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa
     6181 catccagaat tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc
     6241 agaagaagaa gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc
     6301 tattgatggc accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt
     6361 ttatgaatcc ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt
     6421 ttggggtgga aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa
     6481 taaatcctta tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata
     6541 tggaatccga gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct
     6601 tgaactagtg gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg
     6661 ggattatgca gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt
     6721 tggtactcct ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata
     6781 tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca
     6841 aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata
     6901 tttcatgaaa aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa
     6961 ccaataaaaa aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt
     7021 attgcccacg ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat
     7081 acattagacg cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa
     7141 gaacgtggta tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta
     7201 aacatcatgg atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa
     7261 atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt
     7321 tttgtattga aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt
     7381 gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa
     7441 ttaggagcag atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt
     7501 acgtcaagct tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat
     7561 acaattattg aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa
     7621 gtttcattac ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga
     7681 ggaacaatca aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa
     7741 ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca
     7801 aaagctggag acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt
     7861 actcctgtgg atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa
     7921 atgactttct tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct
     7981 cgtaagattg aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa
     8041 ccaacaaact ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc
     8101 ttgattgaaa acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt
     8161 gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca
     8221 gaagaatata tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat
     8281 atggtccata caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg
     8341 attggttatt caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact
     8401 tttgaccaat atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt
     8461 gtttcgatcg atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt
     8521 acagtcttcg tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc
     8581 cgtgataatg atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct
     8641 gctactaaag accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct
     8701 ttggaattct tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga
     8761 aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta
     8821 aaaggatgaa gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa
     8881 ctcttttctg tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa
     8941 gacgtttaaa agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata
     9001 atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag
     9061 aaaggagcgg gaagatgaac gacttattaa aacaatactt tggttatgat gagtttagac
     9121 ctggtcaaaa agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc
     9181 ctaccggaag tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta
     9241 ctgtggttgt gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat
     9301 taggtatacc cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct
     9361 tccaagaaat cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca
     9421 tgccggattt tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg
     9481 cacattgtat ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc
     9541 agctagatca actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc
     9601 aagtagctgc tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg
     9661 gttttgaacg agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc
     9721 taatcgaata tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa
     9781 aagaagtaga ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc
     9841 atggtggtct aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct
     9901 tgcagctgat tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt
     9961 ttgttatcca ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg
    10021 cgggaagaga tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag
    10081 tccaaaagtt ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg
    10141 aaaaattaaa agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc
    10201 taaactattt tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca
    10261 gagaaatagt agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg
    10321 gtgagaacta tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc
    10381 tgcaagcttt aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa
    10441 aagaaacgat gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg
    10501 gtgagtatcc tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat
    10561 ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac
    10621 tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt
    10681 atgtcgtatt ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat
    10741 tggagatgct tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct
    10801 ttttatctcg gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac
    10861 gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa
    10921 tatgttataa ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa
    10981 acttgcgatc caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca
    11041 aaacaacagt ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat
    11101 gtacggcttt tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga
    11161 gggccagctg atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta
    11221 ccaagaaatt tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg
    11281 aaaaaaaggc atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg
    11341 ggattgatca tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg
    11401 tccgcatcag tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta
    11461 ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca
    11521 gttttgacga ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa
    11581 ggttggctgc aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct
    11641 atctggtacc tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt
    11701 gggactgtga aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt
    11761 cccgattttg gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc
    11821 ggagtcaatt atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt
    11881 acgatctggc tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc
    11941 tataatcgat ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa
    12001 ctagtcaacg gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac
    12061 agtatccaga aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta
    12121 gtggaagaac tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt
    12181 gtgcgcattg tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt
    12241 gggatcggcg caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt
    12301 attcctttga cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg
    12361 ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta
    12421 ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag
    12481 gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg
    12541 aatctatgca gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt
    12601 agtaggacaa ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat
    12661 cgcgaaagaa tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt
    12721 gaattttgcg caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca
    12781 tttagatatt tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc
    12841 ttcgattcca ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa
    12901 acaatacggt tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg
    12961 tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa
    13021 agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat
    13081 cgaagtacag attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg
    13141 ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga
    13201 acagcaacgt caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt
    13261 caacgcaggt acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa
    13321 ccctcgcgtc caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac
    13381 aacgcaactg ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca
    13441 agaaggaatt aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt
    13501 gaataacttt ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg
    13561 cgtacgtctg gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc
    13621 attgctagta aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga
    13681 acgttgcttg aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa
    13741 tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac
    13801 agcaacatta tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat
    13861 cggtgaaatt actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga
    13921 agaaccacgt atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt
    13981 attagatgcg aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt
    14041 gttgacggat acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac
    14101 aaaggatttc aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag
    14161 tgagatgtgg gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg
    14221 gcaacggttg agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag
    14281 aggttcaaat gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa
    14341 agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc
    14401 acaaatggaa aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat
    14461 ttgctatact ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa
    14521 agacatggca aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc
    14581 tgggctgctt aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga
    14641 tctgccaatc catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc
    14701 tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac
    14761 tagtcagcct agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga
    14821 aatcacgatt gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta
    14881 caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc
    14941 aggaggacag tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg
    15001 ggatgaaatc aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt
    15061 aactccatca tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac
    15121 agaagaagat atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt
    15181 cttccaagga gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat
    15241 tcttaaaggc agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt
    15301 caacgaagta aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt
    15361 attaagctat ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt
    15421 tggcgatgtt acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac
    15481 aatcaatgta caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga
    15541 acccgatatc gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat
    15601 cactatcaaa gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac
    15661 aaaccgagaa caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa
    15721 aggcgataac gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac
    15781 ggccatcgaa gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac
    15841 tatttcgtct ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga
    15901 aacatgaaaa gactgggttt gttttggctg attctgctat tagttattgt ggcattctac
    15961 atggaaccag tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata
    16021 tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat
    16081 atcggaaaac cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact
    16141 agcggatttg gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa
    16201 gcgaacatcc aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat
    16261 atcgcgccgt tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat
    16321 actaatttca cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat
    16381 atgaattatc gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat
    16441 caaacaacaa acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta
    16501 ttgccatatc aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat
    16561 tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga
    16621 agagaagacg aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg
    16681 ctactacatg attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat
    16741 tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa
    16801 ttggagaagt taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg
    16861 gcgattgacg ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt
    16921 ttcatggacc cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc
    16981 ttattgcaag aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt
    17041 tcaactggag gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc
    17101 ttcttatctt gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga
    17161 agcgttcttg tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc
    17221 tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga
    17281 gcatgttgca gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt
    17341 ccaagtactt gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt
    17401 tgaaacgaaa aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat
    17461 ttgaaataac aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga
    17521 atctgaaaaa gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt
    17581 acttaaatga agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc
    17641 gaaacgtaga acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa
    17701 acaaaacagt attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat
    17761 tagctgaaca tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata
    17821 acgatcgatt tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt
    17881 ctaaggagtg agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt
    17941 ttagcagggg cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg
    18001 attggaccat attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta
    18061 gctatcgaag cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa
    18121 gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca
    18181 ataaaacttg atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac
    18241 tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg
    18301 gaagaactgg gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc
    18361 gaattgccag aaactgtggc tggattccat caaataagaa aacagattta tggcataacg
    18421 gccgtaacta tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt
    18481 ttgatccttt tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg
    18541 aggtagtaat tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa
    18601 gaatgacatt gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag
    18661 aaaatcaatt gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca
    18721 tacgaagtat caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat
    18781 atgatgaatt ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca
    18841 gtattttaaa agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga
    18901 tcaacgaagc tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca
    18961 gtactgatta tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat
    19021 tatgtagaat caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag
    19081 gaagataaag gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt
    19141 ttcttagctt taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg
    19201 actggtgggg cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc
    19261 caaaatgcag caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc
    19321 aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt
    19381 ggagatacag taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat
    19441 tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa
    19501 gaaaaaacag cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt
    19561 atcacgctta ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc
    19621 tctatcggtg ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact
    19681 ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga
    19741 gaagttggcc gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta
    19801 gagattttct ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa
    19861 ataaaaacca actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa
    19921 atcgtaccag taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac
    19981 taaataatta
//
LOCUS       DAJSVP010000044        19818 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSVP010000044 DAJSVP010000000
VERSION     DAJSVP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.044"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            257..532
                     /locus_tag="ON449_001603"
     CDS             257..532
                     /locus_tag="ON449_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934191.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="ON449_001604"
     CDS             545..2617
                     /locus_tag="ON449_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3934192.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="ON449_001605"
     CDS             2782..4668
                     /locus_tag="ON449_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3934193.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="ON449_001606"
     CDS             4680..5627
                     /locus_tag="ON449_001606"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3934194.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ON449_001607"
     CDS             5647..6174
                     /locus_tag="ON449_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3934195.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ON449_001608"
     CDS             complement(6237..6890)
                     /locus_tag="ON449_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3934196.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ON449_001609"
     CDS             7022..7864
                     /locus_tag="ON449_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3934197.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ON449_001610"
     CDS             8022..8918
                     /locus_tag="ON449_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3934198.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ON449_001611"
     CDS             8921..9634
                     /locus_tag="ON449_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3934199.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ON449_001612"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ON449_001612"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3934200.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="ON449_001613"
     CDS             10169..10396
                     /locus_tag="ON449_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3934201.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="ON449_001614"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="ON449_001614"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3934202.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11682)
                     /locus_tag="ON449_001615"
     CDS             complement(11182..11682)
                     /locus_tag="ON449_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3934203.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="ON449_001616"
     CDS             complement(11961..12359)
                     /locus_tag="ON449_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3934204.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="ON449_001617"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="ON449_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3934205.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="ON449_001618"
     CDS             13472..14239
                     /locus_tag="ON449_001618"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3934206.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="ON449_001619"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="ON449_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3934207.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="ON449_001620"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="ON449_001620"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3934208.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="ON449_001621"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="ON449_001621"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3934209.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="ON449_001622"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="ON449_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3934210.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6874 a         3414 c         4121 g         5409 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tctggcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaacaa
//
LOCUS       DAJSVP010000045        18959 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSVP010000045 DAJSVP010000000
VERSION     DAJSVP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.045"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            312..821
                     /locus_tag="ON449_001623"
     CDS             312..821
                     /locus_tag="ON449_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3934211.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(896..1435)
                     /locus_tag="ON449_001624"
     CDS             complement(896..1435)
                     /locus_tag="ON449_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3934212.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(1575..2186)
                     /gene="rpsD"
                     /locus_tag="ON449_001625"
     CDS             complement(1575..2186)
                     /gene="rpsD"
                     /locus_tag="ON449_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3934213.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            2448..3299
                     /locus_tag="ON449_001626"
     CDS             2448..3299
                     /locus_tag="ON449_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3934214.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            3327..3962
                     /locus_tag="ON449_001627"
     CDS             3327..3962
                     /locus_tag="ON449_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3934215.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            3982..4755
                     /locus_tag="ON449_001628"
     CDS             3982..4755
                     /locus_tag="ON449_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934216.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            5163..5960
                     /locus_tag="ON449_001629"
     CDS             5163..5960
                     /locus_tag="ON449_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3934217.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            5938..6351
                     /locus_tag="ON449_001630"
     CDS             5938..6351
                     /locus_tag="ON449_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3934218.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            6335..8980
                     /gene="mutS"
                     /locus_tag="ON449_001631"
     CDS             6335..8980
                     /gene="mutS"
                     /locus_tag="ON449_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3934219.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            8998..11106
                     /gene="mutL"
                     /locus_tag="ON449_001632"
     CDS             8998..11106
                     /gene="mutL"
                     /locus_tag="ON449_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3934220.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            11128..11688
                     /gene="maf"
                     /locus_tag="ON449_001633"
     CDS             11128..11688
                     /gene="maf"
                     /locus_tag="ON449_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3934221.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(11773..13761)
                     /gene="treP"
                     /locus_tag="ON449_001634"
     CDS             complement(11773..13761)
                     /gene="treP"
                     /locus_tag="ON449_001634"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3934222.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            13984..14697
                     /gene="treR"
                     /locus_tag="ON449_001635"
     CDS             13984..14697
                     /gene="treR"
                     /locus_tag="ON449_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3934223.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            14774..15220
                     /gene="msrB"
                     /locus_tag="ON449_001636"
     CDS             14774..15220
                     /gene="msrB"
                     /locus_tag="ON449_001636"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3934224.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            15390..15992
                     /gene="ruvA"
                     /locus_tag="ON449_001637"
     CDS             15390..15992
                     /gene="ruvA"
                     /locus_tag="ON449_001637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3934225.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            16005..17006
                     /gene="ruvB"
                     /locus_tag="ON449_001638"
     CDS             16005..17006
                     /gene="ruvB"
                     /locus_tag="ON449_001638"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3934226.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            17035..17613
                     /locus_tag="ON449_001639"
     CDS             17035..17613
                     /locus_tag="ON449_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3934227.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            17665..18147
                     /locus_tag="ON449_001640"
     CDS             17665..18147
                     /locus_tag="ON449_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3934228.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            18264..18638
                     /locus_tag="ON449_001641"
     CDS             18264..18638
                     /locus_tag="ON449_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934229.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         6259 a         3408 c         4114 g         5178 t
ORIGIN      
        1 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
       61 atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac gatattcaaa
      121 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
      181 gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga cacttcagtt
      241 ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat cttgcaaagg
      301 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
      361 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
      421 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
      481 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt gcttctcctc
      541 ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg cttatcaact
      601 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca gcatgcgtat
      661 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
      721 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
      781 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
      841 tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt tttgattaat
      901 ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa gctgctccag
      961 ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat gcagttgtaa
     1021 aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca gcaagagagc
     1081 ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg atcatccaac
     1141 cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc ccaaataaaa
     1201 cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca agtaaaagat
     1261 aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt aatccaatag
     1321 caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga gaaaggacag
     1381 aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg ttcataacgc
     1441 cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac aaagtgatta
     1501 ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg tctttttcaa
     1561 gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc ttcgtcgatt
     1621 tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa tttttcagcg
     1681 tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt tactgttacg
     1741 atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg gtatgaaggg
     1801 atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg acgtgcttga
     1861 cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg ttctagtaag
     1921 atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac gaacaagtta
     1981 cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc agttaattgc
     2041 atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg tgcgtaaggg
     2101 cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg agaaattttc
     2161 catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa gtttttggag
     2221 taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct tcacctttgc
     2281 agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga gttattattt
     2341 ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg aatgtcaaga
     2401 taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg agagggtatg
     2461 tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg gtatcaccat
     2521 tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa aacagctttt
     2581 cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga acggctgtgg
     2641 catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag attttatacg
     2701 catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa ttaggaacat
     2761 cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta agtaaggcag
     2821 ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta tgtggatttc
     2881 taatttctat gactacgcca tttcaagatt taacgttgga caactttatg ttagaatcaa
     2941 tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg tttgcgaata
     3001 tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc ggaaaagtga
     3061 tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac gtgatagcga
     3121 attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat ggaatgacag
     3181 tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt ggtggcggtt
     3241 tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat gtggactaaa
     3301 aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa aatgaggaaa
     3361 aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga gtacctaagc
     3421 cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta tctatttctg
     3481 aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat cagtatgtag
     3541 cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta gtcgtattgt
     3601 tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga aaagtataca
     3661 gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg tttgaaaaaa
     3721 aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat aaacagcaaa
     3781 cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag gaattcttta
     3841 tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc gattgggtaa
     3901 aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt aagtatttat
     3961 aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat tgatggaacg
     4021 ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga aaaattacgc
     4081 aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc gcaagagatc
     4141 attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg ttttttagac
     4201 catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt cgttcaagaa
     4261 gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa gcgttcttct
     4321 tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt cttgcctgaa
     4381 ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt tttcgatcaa
     4441 acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg gcatgagaac
     4501 agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca cttagcagaa
     4561 caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa cgaccgtgaa
     4621 atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga agtacaagca
     4681 gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc attgaaagag
     4741 ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa taagcccagc
     4801 caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc agatgcaagc
     4861 tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa gaaataagaa
     4921 gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt ctgaagaagt
     4981 agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc atagtcccat
     5041 ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt tgtttgacct
     5101 tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa gaggaattta
     5161 gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag atgattactg
     5221 aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta aatggtgaga
     5281 atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg caagacggcg
     5341 tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt gagtttgtaa
     5401 atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct ggacaaggga
     5461 tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag gctcgttctt
     5521 ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa gaagccagaa
     5581 aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa aaacaagcaa
     5641 tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca cacgttcaga
     5701 caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt gggatgacag
     5761 gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag tttctgactg
     5821 cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc ggctgtatct
     5881 tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag atcaacgatg
     5941 atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc ggaaattcaa
     6001 aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat tcgctacaaa
     6061 gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga aaaaatcaaa
     6121 caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc agaaaaagaa
     6181 gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct tgttgtagct
     6241 tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac tatgatacaa
     6301 gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta aaaatactcc
     6361 tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt tgttctatcg
     6421 tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac agcttttgga
     6481 attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt gcggtgtacc
     6541 tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata aagtagcgat
     6601 ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg aagtgatcca
     6661 gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag acaacaattt
     6721 tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg atttaagtac
     6781 gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg aagcctctgc
     6841 attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc gtgaacagct
     6901 aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa acgcggaatt
     6961 tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg gcaaactatt
     7021 gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag cagtagaata
     7081 ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag aattaagcca
     7141 atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg aaacaaaaac
     7201 agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc aggcaaaaca
     7261 aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg aacgtatcga
     7321 tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc gagtagcttt
     7381 cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac aagtgccaat
     7441 gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc tttcagaaat
     7501 gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg atccgccgtt
     7561 acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg atatgtaccg
     7621 ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag agcgtcaaga
     7681 aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt atatagaaat
     7741 tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac aaactttagc
     7801 aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa tcctcgaagc
     7861 agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag aagaagtcaa
     7921 aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag atgtgctaca
     7981 aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg tcagtgacaa
     8041 gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat taggacatca
     8101 ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc tgattacagg
     8161 accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg ttctgatggc
     8221 gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg atcggatttt
     8281 tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta tggtagagat
     8341 gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt tgtttgacga
     8401 actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa tcatcgaata
     8461 tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg aactgacggt
     8521 acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg aaaaagacgg
     8581 agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct acggcataca
     8641 tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag atatcctgca
     8701 tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag aagagaaaac
     8761 taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc aagaccatat
     8821 acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat tatccgaaaa
     8881 tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga cgccaatgga
     8941 tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg gatatcgatg
     9001 gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg agaagtagtg
     9061 gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc cggcagtact
     9121 cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat tgataacgga
     9181 gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac aagtaagatc
     9241 cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga agctttgcca
     9301 agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga aaaacaaggc
     9361 acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc actgcgtcaa
     9421 ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt gaaatacgtg
     9481 aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact ggcattgagt
     9541 catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc aactgctgga
     9601 aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc aaaaaaaatg
     9661 ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc attgcctgag
     9721 gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt tatcaagaac
     9781 ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt cggtcgtttc
     9841 cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa tgttcatccg
     9901 acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat tagtaacgcc
     9961 attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa tctacggttt
    10021 aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt gtcctacgct
    10081 tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga gacaggtacc
    10141 tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa gtctgtggaa
    10201 aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga aaaaacagcg
    10261 aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac agtggaaaaa
    10321 tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga agaacaagca
    10381 gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact ccatcctgaa
    10441 tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa attagaaacg
    10501 gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca cggaacgtat
    10561 ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc ccaagagcgc
    10621 attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct gcaagaattg
    10681 cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa agagcaaaaa
    10741 cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag ctttattgta
    10801 cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg ggagatgatc
    10861 gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc aacagcaatc
    10921 atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc acaagcacgt
    10981 gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca tggccgtcca
    11041 gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat ccaagacccg
    11101 cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca atcaccaaga
    11161 agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc tgatatcgat
    11221 gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc tgaagaaaaa
    11281 gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc cagtgacacg
    11341 actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga ggctgaattc
    11401 atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt tttaaaaaca
    11461 aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc tttgacagac
    11521 gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg ggcatacggc
    11581 atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta cagtatcgta
    11641 ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac aaaaaagcgg
    11701 actatctgat tttcgattga aaatccatga tagcccgttt tttttattga aaagattact
    11761 tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag
    11821 tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat
    11881 gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc
    11941 tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac
    12001 agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc
    12061 atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt
    12121 tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac
    12181 ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac
    12241 tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc
    12301 tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat
    12361 tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc
    12421 gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac
    12481 tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc
    12541 gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc
    12601 agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt
    12661 cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc
    12721 aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt
    12781 ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga atacggcact
    12841 aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc
    12901 gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga
    12961 aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc
    13021 tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat
    13081 catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc
    13141 tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa
    13201 tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg
    13261 gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg
    13321 ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc
    13381 aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac
    13441 ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt
    13501 ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt
    13561 tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc
    13621 ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc
    13681 acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc
    13741 atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt
    13801 gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat taaaaagtac
    13861 gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat
    13921 tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa
    13981 aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa
    14041 tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca
    14101 cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa
    14161 caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg
    14221 actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg
    14281 ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg
    14341 cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc
    14401 gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc
    14461 tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca
    14521 gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca
    14581 gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg
    14641 gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata
    14701 gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa
    14761 gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt
    14821 atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa
    14881 atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt
    14941 cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac
    15001 gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg
    15061 aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg
    15121 tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga
    15181 tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg
    15241 tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga
    15301 aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg
    15361 tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct
    15421 ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag
    15481 gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg
    15541 taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt
    15601 ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa
    15661 gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga
    15721 aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat
    15781 tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg
    15841 ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa
    15901 tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac
    15961 gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact
    16021 tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc
    16081 tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag
    16141 ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag
    16201 gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga
    16261 gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag
    16321 gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt
    16381 attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc
    16441 cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc
    16501 tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac
    16561 aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag
    16621 aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc
    16681 tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag
    16741 cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga
    16801 aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt
    16861 cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc
    16921 aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac
    16981 attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt
    17041 gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa
    17101 tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa tcgacaaaca
    17161 ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca
    17221 ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa
    17281 atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca
    17341 gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa
    17401 caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt
    17461 tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg
    17521 atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt
    17581 ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa
    17641 taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt
    17701 tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga aggaagggaa
    17761 gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac
    17821 cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg
    17881 caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat
    17941 gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta
    18001 agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt
    18061 gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgat
    18121 gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc gatgttagag
    18181 catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct
    18241 tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta
    18301 cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag
    18361 aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta
    18421 ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc aagtatgaaa
    18481 cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac
    18541 aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc
    18601 tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat agaaaagcac
    18661 gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata
    18721 tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg gttgattagt
    18781 tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct
    18841 agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat
    18901 ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagtttt
//
LOCUS       DAJSVP010000046        18773 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSVP010000046 DAJSVP010000000
VERSION     DAJSVP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.046"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            4..2376
                     /locus_tag="ON449_001642"
     CDS             4..2376
                     /locus_tag="ON449_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934230.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ON449_001643"
     CDS             2437..3897
                     /locus_tag="ON449_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3934231.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ON449_001644"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ON449_001644"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3934232.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ON449_001645"
     CDS             6128..6277
                     /locus_tag="ON449_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934233.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="ON449_001646"
     CDS             6358..6954
                     /locus_tag="ON449_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3934234.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="ON449_001647"
     CDS             6967..7866
                     /locus_tag="ON449_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3934235.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="ON449_001648"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="ON449_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3934236.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="ON449_001649"
     CDS             8720..8980
                     /locus_tag="ON449_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934237.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="ON449_001650"
     CDS             9427..9633
                     /locus_tag="ON449_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3934238.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9633..9857
                     /locus_tag="ON449_001651"
     CDS             9633..9857
                     /locus_tag="ON449_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934239.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9872..10309
                     /locus_tag="ON449_001652"
     CDS             9872..10309
                     /locus_tag="ON449_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934240.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10302..11009
                     /locus_tag="ON449_001653"
     CDS             10302..11009
                     /locus_tag="ON449_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934241.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11390..11569
                     /locus_tag="ON449_001654"
     CDS             11390..11569
                     /locus_tag="ON449_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934242.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11662..11847
                     /locus_tag="ON449_001655"
     CDS             11662..11847
                     /locus_tag="ON449_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3934243.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            11881..12312
                     /locus_tag="ON449_001656"
     CDS             11881..12312
                     /locus_tag="ON449_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3934244.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            13249..14523
                     /locus_tag="ON449_001657"
     CDS             13249..14523
                     /locus_tag="ON449_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3934245.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            14577..16004
                     /locus_tag="ON449_001658"
     CDS             14577..16004
                     /locus_tag="ON449_001658"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3934246.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            16017..16889
                     /locus_tag="ON449_001659"
     CDS             16017..16889
                     /locus_tag="ON449_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3934247.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            16984..17979
                     /locus_tag="ON449_001660"
     CDS             16984..17979
                     /locus_tag="ON449_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3934248.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(18584..18691)
                     /locus_tag="ON449_001661"
                     /pseudo
     CDS             complement(18584..18691)
                     /locus_tag="ON449_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6778 a         2983 c         3811 g         5201 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgctcgaat
     3661 ggtacgtacg attttgatcg tttttatcaa ggaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa agcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatt
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag ggagtgagat
     3901 cgtcacgatg aaaactgtca ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagccaccc gaaaagacgg gtattttgag atccaagatc gattgtttcc
     4021 tggtgaaact gtgattacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacattta cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaac gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgc gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagccta ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa atcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgagtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagctg gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cacgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac gggaacagga aaaggcctgt
     9061 ttaggaaggc acaagcgcct tctcaattaa aaaacctttc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
     9181 caagacaaaa taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
     9661 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9721 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
     9781 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     9841 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
     9901 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     9961 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10021 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
    10081 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
    10141 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
    10201 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
    10261 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
    10321 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
    10381 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10441 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
    10501 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
    10561 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
    10621 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
    10681 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
    10741 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
    10801 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    10861 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
    10921 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
    10981 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    11041 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    11101 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
    11161 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
    11221 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
    11281 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
    11341 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11401 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
    11461 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11521 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
    11581 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
    11641 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
    11701 aaaaatggct gggttgagcg aaggcaagag ggatctcatc atcatcttta taaggatggt
    11761 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcaaaaa
    11821 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
    11881 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
    11941 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
    12001 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
    12061 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
    12121 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
    12181 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
    12241 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
    12301 agaaataatt agaactcaaa agagtcagaa acttaaaaat ctttggcttc tttttttact
    12361 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
    12421 cgagctaatt aagttgctcg tggtctttct ttatgaaaac tttttagatc tagataaaaa
    12481 cattgagaca ctttgaaaaa tttttttaat agtaatgggc gcttctatgt caagctgatt
    12541 cttctttcgg taaattaatt ttacttagaa tatcactatc ttgataaaaa atggtgtatt
    12601 cacaaattct ggcataaaaa atacctactt tctagttaag gttcctgaat aagtcgcaat
    12661 ccaattattt ccagctgatt tataactttt taaatataat tttccataat accaataacc
    12721 attacttttt tctgtccaat ttcgttattg agaagatcta gaaggccagc caactttatc
    12781 aaaaatcatc attcctgtca ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga
    12841 aactgttaaa taattttaag taacagttaa gtcgttagtt gggttatcaa tggctattcc
    12901 gtccttatct gaacctcgaa acccaatcat agaaaaagtt ttttgaacaa gttttaacag
    12961 ataaatcaag ttatatacta gaaaattcgt aaaatatatt tttaatgtca taatcgtttg
    13021 atttttctgg attgacattt atttataaaa aatagtgcca ttaaaaaaga aaagtgacac
    13081 accaataaaa gtcatatcct gtgacattat atggcattat ccaaatagaa taaccggttg
    13141 acatgtaagc ggattccttt tataatttta ggagtataag tccttttcta aaaatattaa
    13201 ccggttatca tgaaagggga tacaaatata tcgttggagg ataaaaatat gtcaaaggtt
    13261 aacgtattta aaaatcaatc ctatttacaa agttcagcta cattattact attttttgct
    13321 tcttggggtg tttggtggtc attttttcaa ctttggctaa catctgaatc aaatggttta
    13381 gggttatctg gcagtgctgt aggaacagta ttctcggcaa attcgttagt taccttaatt
    13441 ttgatgttta tttatggaac attacaagat aaattgtata ttaaacgaaa tttattaatt
    13501 tttgcttctg tattagcgac acttgttgga ccatttttta tatggatata cgggccattg
    13561 ctagataaca attttaattt aggcattatt atgggagcac tatttttgtc agctggatat
    13621 ttagcttctg taggagtttt tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt
    13681 gaatatggac aagcaagggc gtggggatca tttggttatg ccttggtagc gcttttggca
    13741 ggatttttat ttgtaaaaaa tcctcattta aacttttggg cgggatcttt ctttggttct
    13801 ttactattgt taaatttatt attttggaac cctaaagttg aacgggaagc aaatcaaaat
    13861 tttaatcaag aacaagctga atcaaatagt attccttctt taaaagaaat gtttgatcta
    13921 atgaaactgc ctcaattatg gacgataatc atctttattg tttttacatg gacattttat
    13981 acggtattcg atcaacaaat gtttccggga ttttatactg gtttgttttc aacatcagct
    14041 aatggtgaaa aaatatatgg gacattgaat gctattcaag tattttgtga agcgttaatg
    14101 atgggaattg ttccaatcat tatgagaaaa ttaggggttc gaaatacttt gttattaggt
    14161 gtaaccatta tgtgtgtacg aattggattg tgcgggtttg cctcgacacc attatctgtt
    14221 tcatgcataa aaatgttgca tgctttagaa gtaccattat ttacattacc aatgtttcgc
    14281 tattttacac ttcattttga tacaaagcta tcagcaaccc tctatatgat aggatttcag
    14341 atagctgctc aaattgggca agtgatttta tcaacaccat tgggaatatt aagagacaac
    14401 gttggctatc aaccaacatt taaaattatt tctcttattg tattactagc aggcatatat
    14461 gcattcttta ttcttaaaca agatgataga gatgttcaag gggatccatt tattcgagga
    14521 taatggaaac caacagaata aaaaacaatt ttatctagaa agtgagcgaa tatattatga
    14581 caaaaagagc tatagaatta acaaatacac gttatcgttt gggatatcat gtgtctgcac
    14641 catcaggatg gattaatgat ccaaatggat tttgctattt tgatgggtat tatcatgtgt
    14701 tttatcaaca tcatccatat agtgctgaat ggggaccaat gcactgggca catgctagaa
    14761 gtaaggattt agttcattgg gaatctttac cacttgcttt gactcctgga gatcaagaag
    14821 atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct
    14881 atacaggaca tcactattat ggagacaatg atccagatca tttttggcaa aatcaaaaca
    14941 tggcgtatag tacggatggc attcacttta ctaaatatga aaaaaatcct gtaatagcaa
    15001 aagcaccaga agataacaca catcatttca gagatccaaa agtatggaaa cacgatgaaa
    15061 aatattacat ggttttgggt agtcaagaaa aagatggatt aggtcgtgct attgtttatc
    15121 agtcgttaga tttattaaat tgggattaca ttggaccaat ttctaaagcg aaggaagtac
    15181 ttacagaagg atttatgtgg gaatgcccag acttttttga attaaatgga aaatctattc
    15241 ttttattatc ccctcaggga attgaagcaa atggaaaaga ttatttaaat ctatatgaaa
    15301 caggctactt tatcggtgat tatgattatg agacagcaaa attcattaga ggaaattttt
    15361 atgaattaga taagggacac gatttctatg caacacaaac aactttatct ccagatggta
    15421 gacgtattgt tatggcttgg atggatatgt gggaaagccc tatgcctgaa aaagtagacg
    15481 gatgggcagg agcattaacg cttcctcgag aattagagtt aattggagat catttgtata
    15541 tgaaacctgt gagagaatta gaacaacttc gagttggcag cggcgttgaa acaaaagtta
    15601 atttggcagg agaaataata atcgctcaag atgcttcatt caaagaagct ctaattgatg
    15661 tactattgac taaatcaaaa aatcaagaaa tcaaattttc attaaaaaca aaagatgaag
    15721 agttaattaa tattttttat tctaaggata aaaatgaatg taccttaact cgtaacggca
    15781 aaaatgatcc aaggtatgga accattcaga tgtgtgatag attatcactt cgagtgttta
    15841 tcgataaaag ttcggcagag atttttatta atgatggcga gcttgttttt acagagcgtt
    15901 attatacaga aaatcaacca agtatgcatt tgagtgttag tgaagaagct tttgtctcga
    15961 gtacaattta tcatctaaaa aaagatgcaa tttcttacga gtaaggaggt tttatgatgt
    16021 taattggtag catagaagca ggtgggacta aatttgtatg tgccgtggga gacgaggatt
    16081 accgagtaca agaccaaata atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa
    16141 ctgttgacta ttttaaacag tttgatataa atgcattagg tattgcatca tttggtccaa
    16201 tagaaattcg aaaaaattca ccaaaatcag gatatattac gtctacacca aaggtgggat
    16261 ggcgaaatgt taatgtgtta ggttatttac aagatgagct gaatgttcca atgtcttgga
    16321 caacggatgt gaacggatca gcttatggag agtacatcat gtccacttta tcgaatgaaa
    16381 aaataaattc attagtttat tacaccgttg gtactggcgt tggtggtgga gctgttgttg
    16441 acggaaaact attaggatcg tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc
    16501 atcctgatga tattgatttt aaaggaattt gtccttatca tggtgattgt ttagaaggct
    16561 tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa
    16621 cggatcctat ttgggatgta atggcctatt atctagcaca agctgtgatt caaacaacat
    16681 tgattatacg accggataaa atagtatttg gtggaagcgt agtaaatgaa aaattgttag
    16741 aaaaaattcg tgttaatgtt aatgaattat tggatgatta cgtggaaata ccaccgttaa
    16801 gtaaatatat tacgcgtcct ttaattaaaa ataatggatc ggcaacacta ggaaactttg
    16861 ctttgggctt gaatcaatta caagaataaa acatagaaaa aatatcgatt atcaggtata
    16921 gtagttctat agagttcttt ttttcgatag ttaattattg cattatttag gagagacaag
    16981 agaatgcgcc caaaattaga agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt
    17041 tcacgtgtat taaacaatcg tggctattta agtcaaaaaa cgattgataa tgtttataag
    17101 gctatagaag aactcaatta tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa
    17161 actaacattg tcgggttact atttcctaca gtagccaatc ctttttttag tgaactcgtt
    17221 gaggcattgg aaaaaaagtt atatgaaatt ggttataagg tattaatagg caattcgatg
    17281 aataataaag aaaaggaaac aaattattta aatcagttat tgtctgatca agtagatggt
    17341 ttaattgttg gtacacataa ccaagggatt caggaatata aatatcagaa tttaccgatt
    17401 gttgcaattg atcgagtgat gaatgaagat attccagttg ttgaatccga taactacaat
    17461 ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg caaaaaatat tattcataca
    17521 aacggtccaa ttgacttaca gactccggct aatcgtcgga gactagccta tgaagatact
    17581 atgaaagcat atcagttaat tccacgaact gtgacattag atttcaatat ttcttatgta
    17641 aagaaaaaac agattttttt ccaaatgttt gaggattatc ctaaaataga tggtattttt
    17701 gcttcaaatg acatcgatgc ggccctaata ttacaagtag caaaagaaaa ggggttaaat
    17761 gtaccagctg atttattagt agtcggatat gatggtactt taatgactcg gagtatatta
    17821 ccagatctga caacagtaat acaaccaatt aatgatatag cagacacagc tgttgctatt
    17881 ttgatgaaac gaataaataa agaagaaaca aaaaaggaat atattttacc tgtcacatta
    17941 tggatgggga agacatcagt aaagaaaaag gacaattaaa tttataaaga ctatcaatga
    18001 taatttcatt attaattcgt gatataaaaa gttgttaaat ctcttataat agactatgat
    18061 acaaaatcat gcttttaatc tgtgtagatg ttgccaaata tttgaaagac atagaacttg
    18121 tcagaaaaac cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa
    18181 acttttcagt ccttctaaca gaatcaattg cagataaagc agataacatt cttctttctg
    18241 gaagaaataa ttagaactca aaagggtcag aaacttaaaa gtttttgatc ctttattttc
    18301 aagtctgatt tgacagattt tcggcagctc atttatttat ggtcagaaat agttgctgaa
    18361 attttaatct attagcaaat agtccagaac gtatgaagag gattcggata tcatttgtca
    18421 ttaacaccat aaatatttat aaaattcgtg ttcacctaca attaaattta atatataata
    18481 ttcgttgtaa gggcttataa ttttttgtta atgaacaaga tatgtaattg atagtgtcaa
    18541 tagctattca aaaatgtagg cttttggccc ataaataaca aggttactta tatgataact
    18601 ttttttcttt ataatcttta atccgataga gctttccagt agtttgaata atcttagcgt
    18661 ggtgcactaa tcgattgatt agtgcatttg tgttcagtcc attgagacat aatagtggat
    18721 agttttactg agaaaattga taaaaaatag agatataatt caaatacgta tat
//
LOCUS       DAJSVP010000047        18591 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSVP010000047 DAJSVP010000000
VERSION     DAJSVP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.047"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            172..516
                     /locus_tag="ON449_001662"
     CDS             172..516
                     /locus_tag="ON449_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3934249.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ON449_001663"
     CDS             517..936
                     /locus_tag="ON449_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR3934250.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="ON449_001664"
     CDS             complement(1028..1378)
                     /locus_tag="ON449_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934251.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ON449_001665"
     CDS             complement(1344..2675)
                     /locus_tag="ON449_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3934252.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ON449_001666"
     CDS             2931..3497
                     /locus_tag="ON449_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3934253.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ON449_001667"
     tRNA            3642..3729
                     /locus_tag="ON449_001667"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ON449_001668"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ON449_001668"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3934254.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ON449_001669"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ON449_001669"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3934255.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ON449_001670"
     CDS             complement(7452..7898)
                     /locus_tag="ON449_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3934256.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ON449_001671"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ON449_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3934257.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ON449_001672"
     CDS             8363..8881
                     /locus_tag="ON449_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3934258.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ON449_001673"
     CDS             complement(8958..9914)
                     /locus_tag="ON449_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3934259.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVSSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ON449_001674"
     CDS             10080..10886
                     /locus_tag="ON449_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934260.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ON449_001675"
     CDS             10883..11872
                     /locus_tag="ON449_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3934261.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ON449_001676"
     CDS             11869..12873
                     /locus_tag="ON449_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3934262.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ON449_001677"
     CDS             13005..13547
                     /locus_tag="ON449_001677"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3934263.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ON449_001678"
     CDS             13567..14265
                     /locus_tag="ON449_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3934264.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ON449_001679"
     CDS             14286..14498
                     /locus_tag="ON449_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3934265.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ON449_001680"
     CDS             14498..15460
                     /locus_tag="ON449_001680"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3934266.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ON449_001681"
     CDS             15478..15873
                     /locus_tag="ON449_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3934267.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ON449_001682"
     CDS             16036..16401
                     /locus_tag="ON449_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3934268.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ON449_001683"
     CDS             16398..18209
                     /locus_tag="ON449_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3934269.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ON449_001684"
     CDS             complement(18271..18438)
                     /locus_tag="ON449_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR3934270.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         5981 a         3143 c         3826 g         5641 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaagaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattattcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acacacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcaa ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcatc tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt ttagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ctcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg attttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaattg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaaag
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat t
//
LOCUS       DAJSVP010000048        17678 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSVP010000048 DAJSVP010000000
VERSION     DAJSVP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.049"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            706..1023
                     /locus_tag="ON449_001685"
     CDS             706..1023
                     /locus_tag="ON449_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3934271.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1227..1592)
                     /locus_tag="ON449_001686"
     CDS             complement(1227..1592)
                     /locus_tag="ON449_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934272.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1800..2282
                     /locus_tag="ON449_001687"
     CDS             1800..2282
                     /locus_tag="ON449_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934273.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2479..3369
                     /locus_tag="ON449_001688"
     CDS             2479..3369
                     /locus_tag="ON449_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3934274.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3694..6165
                     /locus_tag="ON449_001689"
     CDS             3694..6165
                     /locus_tag="ON449_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3934275.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6268..8004)
                     /locus_tag="ON449_001690"
     CDS             complement(6268..8004)
                     /locus_tag="ON449_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3934276.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8001..8705)
                     /locus_tag="ON449_001691"
     CDS             complement(8001..8705)
                     /locus_tag="ON449_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934277.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8836..9339
                     /locus_tag="ON449_001692"
     CDS             8836..9339
                     /locus_tag="ON449_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3934278.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9299..9637
                     /locus_tag="ON449_001693"
     CDS             9299..9637
                     /locus_tag="ON449_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3934279.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9658..11076
                     /gene="ffh"
                     /locus_tag="ON449_001694"
     CDS             9658..11076
                     /gene="ffh"
                     /locus_tag="ON449_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3934280.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11188..11766
                     /locus_tag="ON449_001695"
     CDS             11188..11766
                     /locus_tag="ON449_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3934281.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11770..12291
                     /locus_tag="ON449_001696"
     CDS             11770..12291
                     /locus_tag="ON449_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3934282.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12370..12924)
                     /locus_tag="ON449_001697"
     CDS             complement(12370..12924)
                     /locus_tag="ON449_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3934283.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13177..13452
                     /gene="rpsP"
                     /locus_tag="ON449_001698"
     CDS             13177..13452
                     /gene="rpsP"
                     /locus_tag="ON449_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3934284.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13464..13715
                     /locus_tag="ON449_001699"
     CDS             13464..13715
                     /locus_tag="ON449_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3934285.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13840..14631
                     /locus_tag="ON449_001700"
     CDS             13840..14631
                     /locus_tag="ON449_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934286.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14801..15322
                     /gene="rimM"
                     /locus_tag="ON449_001701"
     CDS             14801..15322
                     /gene="rimM"
                     /locus_tag="ON449_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3934287.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15312..16073
                     /gene="trmD"
                     /locus_tag="ON449_001702"
     CDS             15312..16073
                     /gene="trmD"
                     /locus_tag="ON449_001702"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3934288.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16209..16556
                     /gene="rplS"
                     /locus_tag="ON449_001703"
     CDS             16209..16556
                     /gene="rplS"
                     /locus_tag="ON449_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3934289.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16814..17107
                     /locus_tag="ON449_001704"
     CDS             16814..17107
                     /locus_tag="ON449_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3934290.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5969 a         3074 c         3541 g         5094 t
ORIGIN      
        1 aatatagata taatacaaaa aggcaaagat attttcaaaa cattttttct tgatgtgata
       61 tccttgtctt ttttgtaatg tttttaactt attgcttttg caaaatttat ttacctgttt
      121 tctggatcaa cgatagtgta tattatgcta tccttgtttg gattttagga cgtttaaaga
      181 ggcacttttt tacataactt aatccataga ccttttaagc atgctgtgag ataataataa
      241 agaagagttt aaagcgcacc ccaaaccact tccccataag tgtattacgc tttaaactct
      301 tttatatttg aattcactaa gaagcatacc atatttttga aaaaaagtga gaaaaaaggc
      361 ttataattag gatttagagt aattaattag atgtttattt tataggactg atattataga
      421 ttacaaatag tatcgtattt cgcaatctta aaattatctt cttactatga ataagataat
      481 aaaatttgta tttgtccttt ttaggtaact tattttaatc tatatacctt tctggatcaa
      541 cgaaagtata ctttatatag tcataacgcc gatggtaact atgcactgta acgataatgc
      601 tgctaaaata tctttttacg aaaaacatca caaggaaaaa cttaactatg gtattatcta
      661 aacataattt tttcggtatt taatatttaa ttaggaggga atattttgct tttttatagt
      721 aattttatct taatagtagc gatattatta cttctgaata tatggatttt tgataggagt
      781 agaaatgcaa gtataggttt tagaactaaa cgaagtctgt cttctaaaaa aaattgggta
      841 tactcacaaa ctatttttta tggaggaatt gttttaatta gtttactttc ttcaacactt
      901 tattctttga atattataga tgtttccacc tcaaattcaa tatctattat tggaatcatt
      961 attgctgcaa ttattacaca actgttttta gtgtttggag agaagaaaag aagtaaaaaa
     1021 tagaaaaact agtgatagtt ttggtgatag tgatattaaa aatcagaaaa gatagaaaaa
     1081 gcaggagata gataaaatgc tgatatttca gcatttttta agagtataaa agagaaaaaa
     1141 agtaataaat gtatacatga tataagaaaa acatatatgt ataaaaagaa gaaagtagct
     1201 tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata agataaggct
     1261 accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc
     1321 agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat
     1381 aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc
     1441 ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg
     1501 gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc
     1561 aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt
     1621 attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc
     1681 tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat
     1741 ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa
     1801 tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt
     1861 tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc
     1921 aaggaagcgg atttgtggtt tctaggaatt ttccagcttt gactgttttc tatttagtga
     1981 taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt
     2041 acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg
     2101 gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc atgtatttct
     2161 tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt
     2221 acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt
     2281 gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt tctcatatgc
     2341 tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac
     2401 tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa
     2461 taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt
     2521 ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca
     2581 agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta
     2641 gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa
     2701 ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac
     2761 tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa
     2821 gtagcagtgg tcgggatggc gccaatcgtc aataaagata cagatgtcaa agatatcacc
     2881 aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg
     2941 aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc
     3001 gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga
     3061 acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat
     3121 ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta
     3181 gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc
     3241 cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc
     3301 aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat
     3361 atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact
     3421 atgtttccca catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg
     3481 ttttttgagc taataccgag ggtcagctga ttaaacaccg tttattcgaa aaaaagagct
     3541 acgacataga actgtcgtag ctcttttttt gctatatgac aggaatgaaa ggtgtttgca
     3601 agcttaatgt gaggaacact ttccatatct gttctatact ggataggaga agtttggaaa
     3661 tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga ataaatggat aaatcgaagt
     3721 aaaaaaaaga aagtaacaca aagatcatca gataaagtca tccgtctttt tccatcgtat
     3781 aaagaaggat taacggcaga tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat
     3841 tcagtcgatc ccacattcaa aacaaatcag caaatcgtat tggaaaacat atttacgtat
     3901 ttcaatttga tctttcttat cttagccatt ttgctttgtt tagtagaatc ctataagaac
     3961 ttgacttttt taccagttat catagccaat acaggaatcg ccatttttca ggaaattcga
     4021 tccaaaaaga tcttggacga actaaatgtt ttaaatacag caaaagtcaa tgtcatacgg
     4081 gacgggatag aacaaacaat agatatagaa gaccttgtaa tagatgacat tgttatttta
     4141 gagactggac atcaaattcc agcagatgca gaggtcgtgg aaggaaaact gcaagttaat
     4201 gaggctttat tgacaggcga agcagatgaa ataaccaaag aaataggcga tccattaatg
     4261 tccggcagtt tcattgtttc cggaaaagct tacgcccgat tagacaaagt tggagcagat
     4321 tcgtatattt cccagctgac agtcaaagcc aagacaatgg gagaaggaga acagtcggag
     4381 atgattgctt ctcttaatca attgatcaaa tggattggga tcatcattat tcctatcggt
     4441 atcctgcttt tttctcaaag ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt
     4501 gcaatggaag cagcattgat cggtatgata cctgaaggac tgtacttgct gacgacgatt
     4561 gcattagcat taagtgcgac taaactagca aagcaaagag ttcttttaca caatatgaaa
     4621 agcattgaga cattggcaag ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca
     4681 gaaaataaaa tgtctgttca aaaagtgatc gtctctaaaa aacaagaaat aatcagtcag
     4741 tcagatcaat tggaagcatg cattgctgac tatgcaaaag cgatggcagg agacaatgca
     4801 acaatggaag ctgttaaagc atattttaca aaaaccactg atcaatccta tcaccatgtt
     4861 attccttttt cttctgtcca aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt
     4921 ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg aatatgcgga agagtttgtt
     4981 tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa
     5041 gaaccagtat tgacagaagc agtcacacca ttaggctata ttctgattgc taatcctatc
     5101 agaaaagaag caagagccac atttgattac ttcaaaaaac agcaagtagc catcaaagtg
     5161 atttctggag ataatccgct caccgtatcc aatgtagctg tccaagcagg gatttcacat
     5221 gctgatcagt acgttgatgc atctaaatta tcagaagaag aatatgaagc agctgtcgaa
     5281 aaatatacag tttttggacg cgtcaagcca gaacaaaaga aaatcttcgt tcaattgata
     5341 aagcgtaaca atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa
     5401 gaagcagact gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa
     5461 gtggtcttac ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc
     5521 gttgtgaaca atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt
     5581 ttgctctcac tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc
     5641 acattgatta gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac
     5701 aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga
     5761 ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat
     5821 aaaacagatg tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc
     5881 tataaaatct gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc
     5941 ggtaactttt tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct
     6001 gtatcacttt tgctgttgag cattctattt ttctcggcag attcgatttt taggcatttg
     6061 accacattgg tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt
     6121 cgcgtagcta aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa
     6181 aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact
     6241 ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga
     6301 acctgttcca agatgactgt ctacagtaat cgtaccatta aggagttgca cgtagtcttt
     6361 tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg
     6421 gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg
     6481 gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt
     6541 tgaatattgg acagcatttt caatcagatt tttgataatt ggataaagga tatctgtttt
     6601 agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat
     6661 tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc
     6721 gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg
     6781 gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc
     6841 accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg
     6901 agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc
     6961 atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa
     7021 gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg
     7081 agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg
     7141 ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa
     7201 acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata
     7261 taattgctct tctgtggtag tacacgcccg atacatctta ctcatttgtt cgctcaacgc
     7321 attaatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg
     7381 attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt
     7441 ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa
     7501 aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat
     7561 acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc
     7621 ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc
     7681 tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa
     7741 gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc
     7801 caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag
     7861 ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg
     7921 ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg
     7981 ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta
     8041 atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta
     8101 acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc
     8161 aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat
     8221 tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca
     8281 gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga
     8341 ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt
     8401 ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct
     8461 ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc
     8521 tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct
     8581 aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt
     8641 tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt
     8701 ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta
     8761 caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga
     8821 aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat
     8881 gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc
     8941 atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt
     9001 ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa
     9061 gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa
     9121 cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat
     9181 gacggaagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt
     9241 cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat
     9301 ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac
     9361 agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat
     9421 cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa
     9481 gatcttggag gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca
     9541 ggtattggaa aatatgactg catatattac agataagtac ccggaagata aaaaactact
     9601 tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg
     9661 gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga
     9721 aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa
     9781 gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta
     9841 ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa
     9901 gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca
     9961 actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta
    10021 gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat
    10081 cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt
    10141 gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa
    10201 gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca
    10261 ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt
    10321 gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta
    10381 ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta
    10441 tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact
    10501 gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg
    10561 ctgacattga ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca
    10621 cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg
    10681 atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct
    10741 ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat
    10801 tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga
    10861 attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag
    10921 gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac
    10981 caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc
    11041 aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca
    11101 gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca
    11161 attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt
    11221 atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc
    11281 cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa
    11341 agtgaagcat tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga
    11401 gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc
    11461 gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt
    11521 tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa
    11581 gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga
    11641 ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc
    11701 aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc
    11761 gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca
    11821 acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca aactacttat
    11881 gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca
    11941 aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc
    12001 cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta
    12061 aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc
    12121 aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta
    12181 ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta
    12241 gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata
    12301 aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg
    12361 ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg
    12421 gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt
    12481 tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg
    12541 tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc
    12601 tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt
    12661 caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc
    12721 cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc
    12781 ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag
    12841 caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat
    12901 aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct ataataatct
    12961 tacaaaagat aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga
    13021 tagattactt atttttaaca aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg
    13081 aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac
    13141 atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa
    13201 cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct
    13261 cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa
    13321 gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac
    13381 actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc
    13441 tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc
    13501 gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt
    13561 acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc
    13621 gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag
    13681 tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc
    13741 gagtgcagct tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca
    13801 agaaagcgta tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt
    13861 attccatttg ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca
    13921 catctatcaa gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg
    13981 gcttccacca ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac
    14041 cagttgaaag aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc
    14101 tatctcaata tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca
    14161 gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat
    14221 ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag
    14281 atcgacacaa atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt
    14341 gcagcaggtt caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg
    14401 aataatatag aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa
    14461 gaaaacagtc taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg
    14521 acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat
    14581 caagatccta tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc
    14641 gctgatctga gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg
    14701 catttttgca taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca
    14761 tgctaaaata aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt
    14821 aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga
    14881 tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc
    14941 acctgtggaa ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt
    15001 tgaaggacat ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc
    15061 aaaagacaac tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct
    15121 gcatgtatta gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc
    15181 caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc
    15241 ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg
    15301 actgattgac gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta
    15361 tgggggaatc catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta
    15421 acttccgcga ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg
    15481 gagcaggtat gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag
    15541 ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc
    15601 agaaaatggc agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg
    15661 agggatatga tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg
    15721 tccttactgg gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc
    15781 cagatgtgtt agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg
    15841 aacatcccca atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga
    15901 caaatggaaa tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct
    15961 atcaacgtcg gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt
    16021 tagaagaaat caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc
    16081 taaaatcttc tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca
    16141 cttaactatt acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga
    16201 agaaaaccat gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc
    16261 cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg
    16321 aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa
    16381 cttatacagt acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata
    16441 caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt
    16501 actacctacg tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat
    16561 tgttatcaga ctgagaaaga acccttggta ttcaagggtt cttttttctt agcatgtaat
    16621 ttgaacaccc tgatccagtt ttttctttaa ttattggtag gggagaataa tggattagaa
    16681 taaatctacc tataagtagt agtaaaaaaa agagcactat gataatatct ttcttaagag
    16741 taaccgcttt aataaaaaat ccagaatatt ctaacataaa tggagtattc ttagaatcaa
    16801 aaggagaaag aagatggttt atacttatac ggagcaagaa aagatgaaag ttttagacac
    16861 attcatgaat gatggaaaag taaaacaagt accaagttca acaaaaaaga aatacatcct
    16921 attgaaagaa gtcttgaaac gctttgacca tggagtgact tatacagaaa cagaggttaa
    16981 ttcgatactg ctgaacgtat tttctagtgg cgattatgtc gaacaaagac gttatctaat
    17041 cacatttggc tttttcaaaa gaagttcaga cggtcgggca tatcagatga tgggaattga
    17101 aaattagaag ttttaattta tactcattga aaaaagttca agtatgcgat attggttcta
    17161 caccgcatac ttgaactttt tttgtcatct gtatctataa tacaaagcca agaacgttat
    17221 tcaaaatatg gatagctatg ataaaagcta atgagcgatt cgcagttaaa taagcaatcg
    17281 tcagtaaaat cgttccagga gcgtacatga aaaaatcaat tggatgcgtt acatgaataa
    17341 agataattct cttttatttg tacgaataaa taccaaaacg tacctcctaa aattagattt
    17401 tggacagatg ccctttaaaa agataacaag atttcgttct tgaaacaata tatatgcttt
    17461 tattccaatg aggttgtgaa aacagtattt tgtcctgagc gttagaaaat tgaaaatggg
    17521 gaatagcaac tcgtacttta ccatttttta agtgaataca agatcagcta attgaacacc
    17581 gtttattcgt gatgagggac acaacactgc agtcgacaca tttctctttt atgttcaaag
    17641 ataacaaata tttaaaacaa agaaaaatag tagataat
//
LOCUS       DAJSVP010000049        17333 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSVP010000049 DAJSVP010000000
VERSION     DAJSVP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.050"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..608)
                     /locus_tag="ON449_001705"
     CDS             complement(63..608)
                     /locus_tag="ON449_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3934291.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(620..1312)
                     /locus_tag="ON449_001706"
     CDS             complement(620..1312)
                     /locus_tag="ON449_001706"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3934292.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1331..1615)
                     /locus_tag="ON449_001707"
     CDS             complement(1331..1615)
                     /locus_tag="ON449_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934293.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1628..2191)
                     /locus_tag="ON449_001708"
     CDS             complement(1628..2191)
                     /locus_tag="ON449_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3934294.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2419..3081
                     /locus_tag="ON449_001709"
     CDS             2419..3081
                     /locus_tag="ON449_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3934295.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3205..4401
                     /locus_tag="ON449_001710"
     CDS             3205..4401
                     /locus_tag="ON449_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3934296.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4502..5878)
                     /locus_tag="ON449_001711"
     CDS             complement(4502..5878)
                     /locus_tag="ON449_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3934297.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5915..6781)
                     /locus_tag="ON449_001712"
     CDS             complement(5915..6781)
                     /locus_tag="ON449_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3934298.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6870..7895)
                     /locus_tag="ON449_001713"
     CDS             complement(6870..7895)
                     /locus_tag="ON449_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3934299.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7913..8587)
                     /locus_tag="ON449_001714"
     CDS             complement(7913..8587)
                     /locus_tag="ON449_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934300.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8771..9664)
                     /locus_tag="ON449_001715"
     CDS             complement(8771..9664)
                     /locus_tag="ON449_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3934301.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9738..11435)
                     /locus_tag="ON449_001716"
     CDS             complement(9738..11435)
                     /locus_tag="ON449_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3934302.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11428..12741)
                     /locus_tag="ON449_001717"
     CDS             complement(11428..12741)
                     /locus_tag="ON449_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3934303.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12741..17249)
                     /locus_tag="ON449_001718"
     CDS             complement(12741..17249)
                     /locus_tag="ON449_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3934304.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
BASE COUNT         4848 a         3897 c         3012 g         5576 t
ORIGIN      
        1 tttgttttat gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt
       61 ttttataaaa tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca
      121 acgggatcga aacttgccac ggcatctttg aaaatatgga gtttgtatcc tagattatac
      181 gcatcgactg ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct
      241 gtgatctggc gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc
      301 cgtttgtcta tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa
      361 gagccgtaca attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc
      421 tccggatgaa aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt
      481 tgatcaataa acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc
      541 ccagtagtca gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat
      601 gcgttcatct tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg
      661 atcgtttgcc tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg
      721 tgtcgctcat tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa
      781 cagcggctcc ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga
      841 ttttatctgg cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca
      901 aattcgttgc ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat
      961 atagaggcat tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa
     1021 aataagctac tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc
     1081 cagctgaacc tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg
     1141 taatagatgc taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta
     1201 atgaacctga aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct
     1261 ctctcaaaat ttttacttct tcttccattg cgccgataat accgattttc atctttttaa
     1321 ttccttcttt ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa
     1381 taataagaga tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc
     1441 ttgttttcgt gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt
     1501 tttcttcttg ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt
     1561 gtgcttgttg tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc
     1621 gatccctcta ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg
     1681 catcacaaat caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca
     1741 actccagtac ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg
     1801 catgataaat atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat
     1861 ggctcagcga cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt
     1921 cgggattttg tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt
     1981 ctaaaggttt tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga
     2041 tcccgacacc gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac
     2101 gaacttcgtc tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg
     2161 ttttttcttc aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa
     2221 ctgctttttt ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa
     2281 accttagatt ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt
     2341 ccgtatgcta aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa
     2401 tgacatggag ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg
     2461 ctatttctct ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt
     2521 gtagttttag ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa
     2581 attcctgcac tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat
     2641 gaaatttcat tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa
     2701 atgaatatgc agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa
     2761 gcagcgaaag ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt
     2821 cagtttcttt atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc
     2881 aatgaccatg agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc
     2941 atcgaccagc tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta
     3001 gatgatatcg acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat
     3061 gaaccagaac aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg
     3121 atgtctggct gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac
     3181 agattcaaca aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca
     3241 ccagttaacg atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg
     3301 ctgaccactt cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt
     3361 ttaattgaca aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc
     3421 gatgctaatg atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt
     3481 gaattctcac aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat
     3541 tccttaactg aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga
     3601 gaagggaaca tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca
     3661 gcaaaatatc aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa
     3721 aaagacggtc tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac
     3781 tactttgatg cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa
     3841 acaacgatca ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt
     3901 cagattgcca atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt
     3961 cgcttagcta gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca
     4021 aatcaggcct tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa
     4081 aaccaagtcg tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc
     4141 caagtctctg aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca
     4201 gccatcgaaa cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc
     4261 actcaaaatg atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa
     4321 gaaaaacgtc gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa
     4381 ctgttggatt taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac
     4441 agtttattac aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag
     4501 tctatttctc attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa
     4561 taaccattac gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa
     4621 tacaagtcaa gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag
     4681 cttttggttt gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat
     4741 aggcgagcgc catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa
     4801 agaatacagc tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat
     4861 cgatttctgt cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag
     4921 caagtgaata catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga
     4981 tcaccgcgcc cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga
     5041 ctcctagttc agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga
     5101 cagtagcaat cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga
     5161 cttcctcggc tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg
     5221 caccatctag cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa
     5281 cgaaaaatat tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg
     5341 cattattcaa acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca
     5401 ctacgagcac cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta
     5461 atgcttgtgg aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc
     5521 cggaagcaac agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc
     5581 aggcagcaaa ttcaccaaat acaacataca gataggcata tggcccacca atcactggca
     5641 ctcgcgaagc aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga
     5701 caaatgaaag tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa
     5761 atattcccgt accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt
     5821 cttttttcat ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg
     5881 aagttcctcc ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa
     5941 ggataccacc taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg
     6001 tttttctttc gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg
     6061 aaagtgagaa gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc
     6121 ctaagcccca taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt
     6181 tacggaaagc aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg
     6241 acatcgcatc caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag
     6301 agaagatcaa tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt
     6361 ttccttcgtc atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa
     6421 taataccgta tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa
     6481 tcgtattcaa aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt
     6541 acttcattcc atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc
     6601 cgatcaagaa aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa
     6661 acagaaatgc accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac
     6721 cacttactaa accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca
     6781 taaccaatct ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac
     6841 aagcactttt tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt
     6901 gggaaaaata aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata
     6961 cctaattttt cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg
     7021 ctaagtcgac catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct
     7081 gggataccga tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg
     7141 ctcacaatga tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg
     7201 aagataaaag cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg
     7261 tagcgaatat ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt
     7321 ccttttagtc catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa
     7381 tacaaaactt gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac
     7441 ttttgatcgt caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc
     7501 actttgattt catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt
     7561 gctgattgca ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt
     7621 ttttcttctt ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg
     7681 tcggctaatt ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc
     7741 agaaagacgc cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt
     7801 ggcgccagcc acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat
     7861 gtccactgat ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa
     7921 cgatatatcc ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt
     7981 ttcggcgtaa ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat
     8041 cccacagtgc tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa
     8101 taaataatag acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac
     8161 ttccgttgtc aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg
     8221 tgtagttgta agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga
     8281 aatgttttgt cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg
     8341 tattcctgct ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc
     8401 aataatagcc atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc
     8461 tttcaaagtc tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt
     8521 tctcaagttc ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt
     8581 ttgccataaa aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt
     8641 gaaacaagtt ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat
     8701 atgcgattta atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat
     8761 aattggtcta ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac
     8821 accaataacc gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat
     8881 aaagatggta tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc
     8941 gtcttcctgt cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg
     9001 tgactagatc ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa
     9061 ttttctcaac gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat
     9121 cttgctccat ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg
     9181 taatcacgga atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg
     9241 ctcctctatc taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt
     9301 tcggaatata gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga
     9361 catttctaac cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg
     9421 tatgagcaag cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt
     9481 ctttatcgcg ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc
     9541 tgagagcatc tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg
     9601 ccataaataa ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt
     9661 tcattttctt caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg
     9721 cagcttattt ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac
     9781 gctccaatca tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta
     9841 gcaggaagtg acccgcgttt catattcggt actctttctg gccctggatt ttctactcct
     9901 acgatctgaa tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc
     9961 tcacctgttg gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca
    10021 taatctaacc cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt
    10081 ccgcttgttg cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc
    10141 cctttataaa atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct
    10201 ttaagcaatt cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact
    10261 ttctctggcg gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata
    10321 accgctgtgt tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac
    10381 gtgatcgtta aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct
    10441 actccatttt cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata
    10501 taatcttgtc cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa
    10561 taagttagac caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg
    10621 ttttcaagga cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt
    10681 tttcctcttt ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc
    10741 gttacttgtc taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg
    10801 tttttcggat acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac
    10861 acgccatcca ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt
    10921 ctggaaatat gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa
    10981 ggtgcttctt ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt
    11041 tgtttgtcaa tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga
    11101 atccattcaa tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta
    11161 tctcgaatca aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga
    11221 atattttctt caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca
    11281 gatgagaaac cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct
    11341 tcggctttga ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt
    11401 acactcattc ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat
    11461 taataaaaca gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc
    11521 attcgtttgc gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg
    11581 ctctttcggt ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga
    11641 ttcataggca gagactggaa ctttcccgga ttctaattca tccatctcct gtccgttaac
    11701 tactacatat tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat
    11761 tctcttactt gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata
    11821 ggtgccagaa ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa
    11881 tttgcttaca tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt
    11941 caagtcatta gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag
    12001 agcaaaaaca gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata
    12061 cttgaagaag tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg
    12121 cagttcctcg cttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc
    12181 tgtctgatcg atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa
    12241 ataaactgca tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc
    12301 taatccgcca accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc
    12361 ttctgtgatt agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc
    12421 tttaggagtc atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat
    12481 ggcaaatgtc gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc
    12541 ttcctcagag atagccaagc catcattttc atggtattca aattgatcgt tataatcgat
    12601 atctcgaatc atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc
    12661 cgacatcgcc tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat
    12721 caaacacaac agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa
    12781 ccataggctg ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt
    12841 ccggatcctc ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat
    12901 ctgattcgat ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct
    12961 gcttcttcgt ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga
    13021 actacccgat cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa
    13081 cctggaagtt ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc
    13141 aacaagtctg aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg
    13201 atgatttcat actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga
    13261 cgtcggctat tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct
    13321 tctaaggtta cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt
    13381 tcgtcttcga tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg
    13441 aacacttgat cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca
    13501 ccaatagaca gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg
    13561 gcacgagaat agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga
    13621 ttaccagtgt atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa
    13681 cctgcaccat ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa
    13741 tcaaacgtat tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac
    13801 gtgagatatt gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta
    13861 tcgtaaagtc gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc
    13921 tggctctcaa tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca
    13981 aatgaatctg gctctccgat tggatgaata actttctcac catccacgat tacatatttc
    14041 ccgtcttcat ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca
    14101 ccagaaagcc ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa
    14161 agttcaaact ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc
    14221 atgtggattg gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct
    14281 gtgccttcta tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag
    14341 gaattcggat ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca
    14401 agctcgtagt gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga
    14461 tacgtctcat atactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg
    14521 tcgctgactg ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt
    14581 ccgtccgtgg tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg
    14641 tcatataatg caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact
    14701 tggaaattac ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca
    14761 gtagtgatat cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg
    14821 taaaaatttc cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt
    14881 tccccttcac ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac
    14941 gttgtcggag tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt
    15001 tctggctctt ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct
    15061 gccgatctgc cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct
    15121 tgctggatcg ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc
    15181 ttgttgttcg cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt
    15241 ttcaatacat ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt
    15301 cctaaaccaa tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga
    15361 tgataagcaa ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta
    15421 ttgttattag aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc
    15481 tctccataac gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag
    15541 gtaactggat gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg
    15601 aacaaaacgt cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct
    15661 ccattagtgc tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca
    15721 tctcccacaa cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac
    15781 aatgtttcat agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga
    15841 tttaatgctg tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg
    15901 tccgaactga tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata
    15961 ccgattcgaa tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc
    16021 tcaatcaacg cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc
    16081 gctgacttat ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg
    16141 atgacatcta gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct
    16201 gtgctgcttg gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag
    16261 taactgtttt caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt
    16321 ctatcttctt cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc
    16381 gctcccccgt ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct
    16441 ttgtctcctt gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca
    16501 ttcgttgtaa atttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata
    16561 tattcatagg ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac
    16621 tggcgttgaa tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc
    16681 atctcatttt cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct
    16741 tgtaattctt ggttatctat tctttcatca accaccactt gaagaggtag ttccccttcg
    16801 ttcacatcgg ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa
    16861 taaacttttc cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca
    16921 tcttcatcat aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg
    16981 gctgccgcat cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa
    17041 gccagatgcc attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag
    17101 tcctcatcta agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg
    17161 gtcgtcatat gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac
    17221 tgcaaaagct tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct
    17281 tctttcacaa caatcaacaa aacaatgccc atctaattat aatttaattt aat
//
LOCUS       DAJSVP010000050        17130 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSVP010000050 DAJSVP010000000
VERSION     DAJSVP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.051"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            143..215
                     /locus_tag="ON449_001719"
     tRNA            143..215
                     /locus_tag="ON449_001719"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Lys,seq:ctt)
     gene            377..598
                     /locus_tag="ON449_001720"
     CDS             377..598
                     /locus_tag="ON449_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934305.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(922..1167)
                     /locus_tag="ON449_001721"
     CDS             complement(922..1167)
                     /locus_tag="ON449_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934306.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1161..1562)
                     /locus_tag="ON449_001722"
     CDS             complement(1161..1562)
                     /locus_tag="ON449_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3934307.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1734..3542)
                     /gene="uidA"
                     /locus_tag="ON449_001723"
     CDS             complement(1734..3542)
                     /gene="uidA"
                     /locus_tag="ON449_001723"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR3934308.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3570..5336)
                     /locus_tag="ON449_001724"
     CDS             complement(3570..5336)
                     /locus_tag="ON449_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR3934309.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(5349..6143)
                     /locus_tag="ON449_001725"
     CDS             complement(5349..6143)
                     /locus_tag="ON449_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3934310.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(6154..7530)
                     /locus_tag="ON449_001726"
     CDS             complement(6154..7530)
                     /locus_tag="ON449_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3934311.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(7659..8306)
                     /locus_tag="ON449_001727"
     CDS             complement(7659..8306)
                     /locus_tag="ON449_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3934312.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(8390..9817)
                     /gene="uxaC"
                     /locus_tag="ON449_001728"
     CDS             complement(8390..9817)
                     /gene="uxaC"
                     /locus_tag="ON449_001728"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3934313.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(9891..10970)
                     /gene="uxuA"
                     /locus_tag="ON449_001729"
     CDS             complement(9891..10970)
                     /gene="uxuA"
                     /locus_tag="ON449_001729"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3934314.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10973..12604)
                     /locus_tag="ON449_001730"
     CDS             complement(10973..12604)
                     /locus_tag="ON449_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3934315.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12921..13592
                     /locus_tag="ON449_001731"
     CDS             12921..13592
                     /locus_tag="ON449_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3934316.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(14428..15384)
                     /locus_tag="ON449_001732"
     CDS             complement(14428..15384)
                     /locus_tag="ON449_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934317.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(15493..15738)
                     /locus_tag="ON449_001733"
                     /pseudo
     CDS             complement(15493..15738)
                     /locus_tag="ON449_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15883..16305)
                     /locus_tag="ON449_001734"
     CDS             complement(15883..16305)
                     /locus_tag="ON449_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934318.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(16485..16856)
                     /locus_tag="ON449_001735"
     CDS             complement(16485..16856)
                     /locus_tag="ON449_001735"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3934319.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         5421 a         3404 c         2375 g         5930 t
ORIGIN      
        1 ttaaatcagt aattttcaat atcgatggat aactttcaaa tattttttca taatcttgaa
       61 aatccataaa attcactctc aattctgata ataagtattg cactttttta ttttaggtgt
      121 tagaataaca aagtattaat ttgggctgtt agctcagttg gtagagcaga tgactcttaa
      181 tcatcgggtc gcgggttcga gcccctcaca gcccattggg tgccaaaccc ttaaatacag
      241 tgtaaacgtt ggtgcttagc atttagaaaa cgtatatact gtattttttt gttttcaaaa
      301 gagactgtag tacccctttt tttcactaaa agcccactgt agtcattgat atttttgata
      361 agaaaggaac ataattatgc gtaatgaaat tgctaatata acaataatcc gagaaacttt
      421 acaaaaccat actgcaaatg agatctctaa acatacagga ttaaatttga gtactataaa
      481 aaaactaaaa agtggagaac gttcaataga aaaactaaat ttacatgatg ctatttgcct
      541 cactgaattt ggtcttaaaa ataatagaaa aaatgttgaa atcaacatat ggaaataatt
      601 cttaatcagc tgcttttgtc aaaagcacat aggaatttca cctctctcca agtttacggc
      661 gagccttacg gaagtatcat tatcccaact tcaaatcatc gctttttatt gactataaaa
      721 attattttag aatgtttatc gctccttctt tacagtaaga aatcatggcg caactaaaat
      781 atgctccttg aagtgttaac gtactgatag aatgtattta aatttcaatg atcaataggt
      841 tttctgttta aaagaatcta ctaatccttt aactattaga agattctttt aaacagaata
      901 agtggaaagg ggacttttta gttagttaat tttaaatttt aagatcgctt ctatcaattt
      961 aatttctagc tgtcttagca tatactcatc tacataggta ataaattgcc ccgcctcatt
     1021 aaatccattt gtcaaacata acttagctat ataactatga taatgtttga gtatcatctt
     1081 gattgaaatt ggatcttctt ctagagcaga ttggataatg gatagaggaa ctaatggata
     1141 ctgccgtttt ttggggttgt ctaccataag tcatacccca tttttaattg ttcaactgcc
     1201 ttctttcgtt gataccagat accacttgta ctcatattga ggattttacc gatttcagta
     1261 tcattaaaac ctgcaaaata ataaagtagg ataacctttc ttcttatttc cgataaatga
     1321 tcaatggctg ccgataactt ttgatctgaa attaatattt ccattccaaa taagaaatag
     1381 acatcttgtt catcaaaatg agtttgggag tacttacctt catttgaatt cgtcaatggt
     1441 tcctcattat ctgttatgtt aatgtttcgt tttcgaatag acttagtttc gtttcgtaat
     1501 acttttttac agtaggcatc aaattgatgt tgtatagatt tctcattgta attttgttgc
     1561 atgataaata tgctcctttt ttcccctttc cactaataca acaatcagaa agcgcctttt
     1621 tttcgaaaaa actagaaaaa aatatccttc tttaaacaga aaatcttttt ataaaaaaaa
     1681 ggagttggca attaagcagc cactcctttt ttatattcct tataaaaggt aaattatttt
     1741 ttataactga aatttggtat tgatgaccat cgtttcttta gatagtgagc aatcatttta
     1801 ggctttctat ctctagtaaa gattcctttc ttatttcctt gcactcttaa aatcccttga
     1861 cttgtttcaa aatctgcaaa attccacact tgttcaccaa caaaattgtc acatttatct
     1921 attacttcgt gatttgcttc gtaataaccc acttgatact cttctgtaaa cattgttggt
     1981 actgtatcat gaagtccagc tactgtatct gctccatatt ctgtaaacat tattggcgta
     2041 tcgggacatc tttccatcca tccatctagc tcttttttca acagatattt tgcatgtttt
     2101 aggtctccac ctgaaacata ccaaccataa tagcgattta aacataaaac atcgacaata
     2161 tctcctactt tacaaatatc tggtgttgac tttaagtaag taacgattgt tacaggtctc
     2221 ttctgagggt caagttcttt agtcaattca actaaaggtt caaaatattc tttagcgcct
     2281 tcagaatccg aatctggttc atttgcaata gaccacatta cgacactcgg atggttcttg
     2341 tcgcgttcta tcaattcctt aatcgccatt ttgtgagcat tttctgtccg tatttctttc
     2401 caagtatcat gtttttctac gttgtcatct aataaagtag ccatgaaatt caaatgtaat
     2461 cctactgcag gtacttcatc aataactaca ataccttctc gatctgctaa gcgcataatt
     2521 tcttctgaat aaggataatg tgaagtccta aatgaattag caccgatcca tttcattaaa
     2581 ttaaaatctt tgatatttac tgcttcattc aaaccacgac cattaatata cgtgtcctca
     2641 tgcttcccaa atcccttaaa gtaaaaaggt ttattattaa tcaaaaactt cccattcgaa
     2701 acctgaatac tacgaatgcc aaattcttct gtgtacgtat caatcacttt attttcgtta
     2761 attaaactaa ctttcatact atataagtaa gcatttagag gctcccaaag tgttgggttc
     2821 ttaagttgaa tcactccttc tttcccctta gcttctgcta taagttgagc ttcttcgtca
     2881 tagattgcta ttagaatttc tccggtccat tctgtatcga tattaaattc tacatttgca
     2941 tctgaatcaa ttatttttgt attaatagtg acatccttta tatagtcttt cggagttgta
     3001 tacaatttaa cgggtctatt aatgcctgca taattgaaaa agtcaaaatt aaccaaattt
     3061 tcttttttta tgtctccatc ttcctgtatc gtttctttca aaatccctac aggtaaagtg
     3121 ctatcgtcaa taacattgtt tacagccact gtaattctgt tacttccatc ttttataaac
     3181 tgattaattt ctgattcaaa tggcgtaaac cctcctttgt gttgattaat caattttcca
     3241 ttcaaaaata cttttgcttc atgtgttgca gatccaaaac gaagaactaa tctttcttct
     3301 aataattttt taggaagaat cacttctttt tcataccaca cccatccaac atgatttcta
     3361 atgttctctg tttcaaataa atcattataa gaagaaggaa ctgaaattgg ataactctct
     3421 ttttcaacca ttggttctaa agcaagttct tcttgtaagt catcatttgt atctaactta
     3481 aatctccaaa gaccgcttaa atcaattact tgcctcgtct ctgtcataat gggatataac
     3541 atgtaaatca ctcctatata atttaaatat taaataaatg tatcctttaa cccacaataa
     3601 gcatctattg gacttattcc cttaaattca gaaaggccca ttagtttgtc cactaatatt
     3661 tctaaagttt cttgttttcc atcataagta ttgatataag tttttacttg aggcacatca
     3721 gctaaatgaa acggacactg tacagataca aaaattgttg gaatttcatg aatataaaat
     3781 ggaatatccg gagtaccttt acttgctggc caaacaattc tttgaactgt acctgggtta
     3841 acattagcta tattaataat caagtcatat ttttcagtta aatctttgat tggtctctta
     3901 gcagcatata ttccagctat atttttaatt ttttcttctt gcgataatcc tttcaattct
     3961 tcttcaggtg aaatgtattt ttcaacttca aatccttttg ctgacaaaat ctgtttcaat
     4021 aaatcaatgg cctgaggtct atttccagca actacttcac caaacccacc ttttacccca
     4081 tgtacatcta ccagtaaaat ccttttatat ttttctggta caataggcca aatatcttgt
     4141 ttatctttaa ctagtgtaat tgcttgatca gctacctctt taaaaattga tttattttca
     4201 attaaaccaa ttttttccat agcttcttgt ttcggtaaca aaattttatt cttttctttt
     4261 ttatgcaaac ctagtttagc ctttaagcct aaaattcttc ttaatgcatc acgtaaacgc
     4321 tcttctgaaa taattccttt gtaataacct tccatcatat aattaaagtc ttcatctgga
     4381 tcattaaaaa ataggaatag atcacatcca gccgcaatag aagtgggcaa taattctgac
     4441 cgtttcattg ccccagtcat tcctaacata tgactagcat cagtaactac taatccatta
     4501 aacccgagtt ttcctctaag taagtcagtg attaattctt ttgataaagt tgcaggaagt
     4561 aaatcttcat ctttcagatc aagattaaaa tgttttgaat aagctggcat atggatatga
     4621 cctgccataa ttgaaggtaa tccagcttct attaagcctg agtatacttt tccaaaagaa
     4681 ttatcccatt cttctgtact taaactatta atactaaaag atagatgttg atctcgttcg
     4741 tcaataccgt caccagggaa atgtttagct gcaggcaaaa ttccactctc ttgaatacct
     4801 ttcatataag ctaaactaag ttctaaaact ttatcagggc tttgtccaaa agatcgacta
     4861 gaaatgatag gatttctcca atttctatta atatctacaa taggagcaaa actccagtta
     4921 catccaatag ccgatgcttc aattccagaa actctcccta attcataagc atatttcgca
     4981 tcatttgttg caccaatttt tacttctaca ccaacttcgg taccatctgt acaagctccg
     5041 ttaccacctg cttctgtatt agcagcaatt aataaaggaa ttttgctttc tgtttgtagg
     5101 atatagtttt gatcatatac ctcatcagca gttcctggat tatatcgtac agctccaata
     5161 tggtactgat tgaccatact acttaagtaa tttttatcac gactagatcc catattaaca
     5221 aataattgac ctattttttc ttctaaagtc atagcatcaa tcgtctcttc aatccaagaa
     5281 atagcatctt catctaaatg atatggtttt tttcgtaagt ctacttttat agtcattttc
     5341 tttctcctct acttattagt aagatttttc caaaaagtat ctatatattt ttgttcaata
     5401 tctatatact ctcccgataa acatttaaat aaaatatcag ttcttaagtt ttcccatgat
     5461 tctttttctt tattaaccaa aataggataa aagaaaactt tattcttatt tgcagcttct
     5521 agatcaccag gagaatcacc aatcattaat atcttatcca aatcacaacc attttcaatt
     5581 acacttcgaa taatgtcttc tttttttcct ttatcttgac aacacagttc atcaactaag
     5641 gataataatt catgtctttc ccattcttct tgaatagctt ccttattagc tgagcttaca
     5701 ataattatct tcccatattt tttcagagct tttatagtat ctctagcact taaaaagggt
     5761 ttatctaatc ctttagcttg actaatctgt cgattgactt cttccgacca ttttaaagct
     5821 tttaataaat cctctgactg agagtattct atttccctct tcaaagatgg attagacaat
     5881 tcttttgtta cagataccca gtgctttaat cgattaatcg atggtacatc atttttttct
     5941 aatgttttaa ctaaacctaa aaatctgttt attccacgag tctctgaata taaattaata
     6001 ttattccaat cctgtaggaa tttttcccta tgatgaacat caaaaatctc tgcagcaatt
     6061 ggcccgaaaa agagattgtg tttataagtc attgtatcca tgacacaacc atctgaatca
     6121 atacaaaata tccaactgtt catgtttttt ctcctagtta tgtttttgat ttagcaaatt
     6181 ataattttct aatacttttt ctttagataa tggatatacg aaaaataaaa tcaatcctac
     6241 aacaaagaaa caaactgctg gaatcaacgt tgaagagtta tacagctttt gtaaaacttc
     6301 tccactttgt acttttgcta acgaatcata ccctattata gaaagagtcc aacctccgac
     6361 accgccagct aaagcttgtc caatttttcg ggcaaaagaa tatactgcat aaatagttcc
     6421 atcttctctt tggccagatt tcacttcttg atcatcgatt acatcagtaa tattcgccca
     6481 aatgactgta ttaaaaatac ctaaccccat atatccaaca gaagtaagaa ctaagaatac
     6541 atacatatta ctaggcttta tcaaaaataa aagtaaatat acaatagcag agaagaacat
     6601 ccctactgac gccaattctt ttttaccgta ttttttactg aaaattaaac ttacaggtga
     6661 aacaagtacc aacatcaaaa taggattgat aaagttcatt aaaactattc cctgtgcatt
     6721 attataataa tctggaaata cataattgtt catactcatt gttaaaagct gagcaagcaa
     6781 caaaaaaata gctgcagcta ttatagaaag caacgctcta tttttagcta atccttttaa
     6841 agatttcaac aatgaaactt ttaactgcga ttcatcactt ttaacccgtt ctacagttaa
     6901 tttataacat attacgtaac aaataactgc acacactgaa aatactcctg ccacaattgt
     6961 aaaagttgat ccaccacgta caatttgagc tccagaacta tctcttgtat aaataaaaat
     7021 tggtgttaac acgccaatga acatacctgc taaggtagct cctatactac gaaatgttga
     7081 taacgctgtc ctttcttctg caacagaagt gatagctgat gccattgaac cgtaaggaat
     7141 attaatagat gtataaaaaa tacttcccca tagtatatat gttacataca tatatataac
     7201 tttaatactc atagaagctg atgccattga tgtttgatac attaaaaaac ttgctaaagc
     7261 gactggacca ctcatccgta aaatccacgg tttaaatttg ccagttttag atggttttga
     7321 tccatccaca atacgcccca ttgttatgtc tgtaactgca tcaataaatc ttgctactaa
     7381 aaataatgtt cctacggtag ctccgcttat ccccaatact tttgtataaa aaatcattaa
     7441 aaaagaacta gcaaaaatga aagtgaaatc attcccaaaa tctccaaaca tatatcctat
     7501 tttgtctctt agaccaaatt ttctttccat ataatttgcg ctccttccta ttgttaagcg
     7561 ttttcattct gttgaactgc caacttacgt actatactct gaagtataaa gagttagtat
     7621 ttatttttta cattttatag tattatttat tttttctcct aataattctt tatctcataa
     7681 aacttatcta tatatttact agctattcga gttacttctt caaaatcacc tttttcaaca
     7741 ggagccaaca aattcccacc tacaccaact gcaacaacgc ctgcttcaaa ccactcttct
     7801 aagttatcta gacttactcc gccagtaggc attatattta catatggtaa tggagcttga
     7861 tacgttttta caacagatgg accaaaagca gaacctggaa atagtttaat gatttctact
     7921 ccagcggtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa ataaggaatt
     7981 tgatataaat tacaaatctc tgctgtttgt cgatcaaaag atggacttac gatatattct
     8041 gctccagcca taattgcaat acgagcagta aacgcatcta aaactgtacc ggctccaatt
     8101 attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc ttcagcattc
     8161 ggaactgtaa aagttagttc aatcccttta attccacccg cagtaattgc atttattgct
     8221 ttaatggcct catctttatt ttttccacgt acaaccgcaa tgactccagc cgcttctaac
     8281 ttacttaaaa tttctacttt tttcatatct atctccttct tttatggata catactctag
     8341 ctagttgtac taacttacaa taaataaatt taagctttta ttttttgtat tattttctac
     8401 tttcgatttc aaaaaaatta aaataattat acgcattacg ataacaaata ttctccacta
     8461 tctgactaag atacatttca tcatctggaa ttcgacctgc tttaacccac tcgcttagta
     8521 attgacataa tactcgacgg aaatactcat ggcgaggata gcttagaaaa cttcgagaat
     8581 cagtcaacat acctgtaaaa ttcgacaaca aactttgttg cgccatcgta gtcaactgtt
     8641 gacgcattcc ttcagctgta tcattgaacc accaagcaca tcccaattgg attttttgaa
     8701 tcataccccc ttggaagctt tgcattgctg ttgctagtgg aagccaatcg gttggattta
     8761 atgaatataa aattgttttt ggtaagcacc cactcttttc agcagtgtcg aacaatgcta
     8821 caacattttg tgtcaaacta gcttgatccc ccatcgaatc accaccgaca tctactccaa
     8881 tttgttcctt taatatactt gaattatttc ttaaagcatt taaatgcatt tgcatcgtcc
     8941 aaccatatct tgtatactca gacatcaaat gtaattgaat acctgtaaca aattgtgcaa
     9001 tttctgataa atggaaatcc gtttttcctg ctaaagcttt tattaacaga ttgtcgagta
     9061 ttttttcatc tgctatttca taaaaataag tattcaaacc gtgatctgct aatcgacagc
     9121 caatttgatg aaaataatcc actcgttggc tcaatgcctg cttcaaccca ttaaagtttt
     9181 tgattggtat acccgctgct tgactaagtt ttaaaatata gttcgaaaat cctgatgatt
     9241 tgatactcca gaccttatct ggacggaaag taggtaatac cttgaaagaa ttatcctgtt
     9301 tagaaagctc agtatgatat cgcaagtcat ctgtaggatc atccgttgta caaatggctt
     9361 tcacatccat catcttcagc aattcttttg ggcgatagga atccgtttgg attttttcgt
     9421 tggcacgtcg ccaaatttct ggtgcattct tctcagaaat tgtcaaatca atatcaaagt
     9481 aacgttgtaa ttccaaatga gaccattcaa aaataggatt acctggcgcc ttctccactg
     9541 tttttacaaa agctaaaaat ttttcataat cattacctgc accactgatc aactcttctt
     9601 ctatcccatt agctcgcatc aatcgccatt tataatgatc tccagctcct ttatcattta
     9661 accatacctg agtcaaattc ttgtattgct tattttctag caaacatgct gggtctaaat
     9721 gacaatgata atcgatgatt ggttggtgtt ctgcaaattt atgaaaaagt cttttagcta
     9781 tttcattctc taataaaaag ttcttatcta aaaacatatt taagcactcc tttccttccc
     9841 ttagttttta gaagagaaaa tttttcttct aaatagactc cattatataa ttatttactc
     9901 gccccctttt caagcgcttc ccaaatacca tttaaataaa ctgcacctag agctcggtca
     9961 tataacccat aaccaggtct accggtttct ccccaaatca tccgaccatg atctggacga
    10021 atatagccat taaatccatt atcgtacatt gtttttaata tcttatacat atctagagaa
    10081 ccatattctg atttatgcgg cgcttcataa aaatcgagtc cattatcact taaatatttg
    10141 atattacgaa catgtgcaaa aggaatccga tcccgttgac aaaattcaga tagaatttca
    10201 tagacatcat tatctggtcg ttctgcaata cttccagtac ataacgtaat accattagaa
    10261 ggagaatcca ctacattaca caaccattct aaatcgtcac gatttttaac aatacgaggt
    10321 aatccgaaaa tagaatatgg tggatcatct ggatgaactg ccattttaat cccaacttct
    10381 tcacatgtcg ggataattgc tttgagaaaa tatgcaaagt tctctcgcaa tttcgcttca
    10441 tcaacttctt tataggcttc taataattgc ttgatttgtg ataaccgatg aggttcccaa
    10501 cccggcaatt caaaattatt agtagaattt tctacttttc taataatttc ctctggatca
    10561 gtaggaatat tttttgaaat ataggctaac gtagaagacc catcatctaa atgataatcc
    10621 aaatctgatt ttacccaatc aaagactggc ataaaattat aacagataac ctcaatccca
    10681 aattctgcca aattgcgaat cgtctgttta taattctcga tgtattgatc tcgtgttggc
    10741 aaaccaattt tgatatcatc atgaacatta acactctcaa taactttcaa ggtaaggcca
    10801 ttggcttcga cttcatcttt taaagcttta attcgttctc tcggccagac ctctccggct
    10861 ggtatatcca ttaatgttcc aacaattcct ttcattgctg ggatctgacg aatttcttgt
    10921 agggagattt tttcttcatt tgccccatac catctaaaag tcatttccat agttactttc
    10981 ctccaacttc tgttatatat ttattgattg ttttacgaac tgcttgagga cctactagca
    11041 tttcgcaaaa gattttttca attttctttc ctatctcaac ttgatataaa tctaaaccaa
    11101 atatttcctt attttctaag atttcctgta ccgcacaatg aatctttgca ggatcttgtt
    11161 cacctaattt caatactgag aatttttcct gcaactctac caataaagga tcaggacttg
    11221 gcacaaaatt ttttccttga tcatcgactg ccaataaata acgtaaccaa gcagcaatag
    11281 cgataggaat aaatgttaga ttttctgcat ttccgtcttt ttcgacatac tttttaattg
    11341 tttcaccata gcgaatcgct actttttgag aagtatctgt tgcaattcgt tgcggtgtat
    11401 ctggaatata gggattaggc aaacgtttat tgattacttc atcaataaat tgttttggat
    11461 caataacgcc tggactttca actactggta agccttctac ataacctatc cctttaatta
    11521 agccaaccaa atcatcatct tgcatttctg cctgaattga tttatacccc aacaaacaac
    11581 ctgttattgc taatgccgta tggagcggat ttaaacatgt cgtaaccttc atgatatctg
    11641 ctttatcaac tgtttctcta tctgtcataa tcacaccagc cgcttccagt ttaggtcgac
    11701 catttggaaa actatcttca atgactagat aatggacttt ttcagtattg gtaaaagcag
    11761 cgatattggt atttttcgca gtatgaatta tatctaagtc ttcgaaaccc gccttcaata
    11821 aatccatttt gacactttcc gaaggatttg gcgtaattct atcaatcata ctccaaggaa
    11881 aagtcacttt ttctttatcc tttaaataag caataaattt ttcagaaacg tatccttttt
    11941 tttgccaagc ttctgcaatc ataaaaatgc tatcttgaaa acgttgacca ttctgtgaga
    12001 aattatcagt agaaaccatc gcaatcggta actctccagc attaaacctg ctatacaaca
    12061 aggatgtcac aatactcatc gtatgttgag gattggtagg tccagatttt atatctgctt
    12121 ccactaccga aaaaaattgt cctttagaat cttttaaact gtatcctttt tcagtaatag
    12181 taaacgttgt aaactgtaat gtaggatctt caaaatattt aaccactctt tgatagtccg
    12241 ttatattctt tggattgcaa tataaactat cagctgttac tgctaaaagt ctttttttga
    12301 gttcaccatc ttcacccata acaatctcca aaatatcatt gttgtagtca tgataagctt
    12361 tttctactac ttcttcatca aatgtctcac acacaactac tccggattct aattcatttt
    12421 gatctgctaa gcattgagca atttctgcat gaaatcctcg ataaagattt cctccaccaa
    12481 aatgaagcca cactggatgt ttctgacctt cttgtttgac cgcttcgaca ttaaaatacg
    12541 gtagttgaac ttgcattttg ttcaatcctt cgatagaatc ttgccagtct ttaatcaaaa
    12601 ccatatttac ccctccatac tgatatgtta gtttccgtta caattgtagt ataaaatcga
    12661 atgtaatcgc tgtcaataag aaaaaactaa aaatattctt ttttctacca tgtcagaatt
    12721 attgtgtctt tttttagtaa aagcacacca atatatattt tttagaacta acttaaatta
    12781 aagcttaatt tctctttgat cttatttgag tattttttgt ttttttaata gctagtatgt
    12841 tactttgaaa tttgatatta tataacgagc atagaactaa aatatcaaat tcaaaaatga
    12901 aattttgaga gggattaaat atgacaatta aaactagtct ccaaaatcaa gcttatcaat
    12961 ctattcgtca gcaaattatc tacgctgact tagaacctgg aaaaaaagta tcagaaaaaa
    13021 aattggaaga aacgctttct atcggtcgta ctccaatacg tgaagcattg attcaactcc
    13081 gtcatcaagg acttgttgat acaattcctc aaagtggaaa ttatatctca ttaatcgact
    13141 tagatttagc aaaaaatgct cgttttgtca gagaacattt agaacgacaa attatgctag
    13201 agtgttgtgc caagatgaat aatcaaaaga aacaagtttt agaaactata attgcagaac
    13261 aagaaaaagc tgttagtcaa aagaatgctc gtagtttttt tcataatgat aatttatttc
    13321 ataaagcttg ttttgaaatt gcagaacggg aagaagtatg gcaatggttg gaagataata
    13381 atacgcactt ggaacgattt cgttggttac gagtaaaaac ggaaggatta aaatgggaaa
    13441 aaattatgga acaacatcat caactattta aagccttgac catgcaagac cctgaagaag
    13501 ctaacttctt aacatccttg catcttcatt taatgcttga tgaacaaaat gttgtattga
    13561 ctactcaccc aaaatatttt aaaaatatat aactgctaaa tttctttttt ttaatttatt
    13621 tacatttctg attactacag aaaactaact acttttttta tgtaaagttc tgtcaaatat
    13681 agtcacttta tattactttc tcttgagtaa aaaaacatgt gaggtcgtaa acaaagattg
    13741 aactatctac attcatactc tataatctgt ttatttgcag actgataata gcaaaagaaa
    13801 acttataatc aaataatatc aacatttgat cacataattt gagattgaat gctgatttac
    13861 ctatgtttta cgtaatggtc tttagttatt tttgctcata aaatgcataa aacacgcgtt
    13921 tttatgtgtg cttgaaattt cttggctcta taatttctaa gactgataaa aatattctat
    13981 cggttctttt tgcaaataaa aaaacatata gaagctccat tagattgaat caccacaaca
    14041 caatcaaagg agcattccgt atgcttgatt ttcattctat aaaactactt ttaggaatca
    14101 aagaaaatca caacggttca aactaaaagc aagaatatcc gaagtaccat tgtttcatcg
    14161 atcatgagaa aagcattcac ctctgtctgc agatgttctt atatcgctct gtcagtccta
    14221 ttaataaagt actgttttgt tttcaaaaaa actattacga gtacttttta agagaatgcc
    14281 ttgcaaatat tcatcacaca ccgttatata agtcaaatag tttctttcag tcgtcaagaa
    14341 aagattctcc tgtccttggc attatcagtt aagtagcaac ttaattctcg gacttgttct
    14401 actctagcaa aatttatttt catcaactta cgtccaaatg tttttttgaa taattacttc
    14461 atcgcgggtc tggaagactt gcctcattcg ataagtcact agcttttggc gtccttcagg
    14521 atttctagta tacaccaact gacatttggg cagcgacgat tgatttaatt cgtttagaaa
    14581 aaggttagcg atttgcttat ttcggccaaa gcgttctggt accgtcagaa ctatcttttt
    14641 cccttcgatt aaataccgcg gattctccag cggtgctaaa agctccctca tagcttcagt
    14701 aaaaagctgt ccttctcgat agccagcatt ctcaagatag catctaaatt cctgctgatc
    14761 agccgctacg attacttttc tatcactaac ttcaccaagg cgctgcaacg tcgtttgaac
    14821 gcatatggcc aagctttttt tgcgggcaca ttccttttcc catagctttt gtcgcctacg
    14881 aatttggaaa tattccagca agttaccttt aaatatagct gaccagaagc ttagaggctc
    14941 tatatttttt actactatcg gcaatgaagt ttcttgtttt tgctttacct gatcgacttt
    15001 cctaacgacg attggcatag ataggtgcca gccatcagct aaagcagtac tccattgttc
    15061 tttcaaccga cttcgttcac aagaaaaata gaacatcgac tgagtataag tttccatcgt
    15121 ggtggtcgta tattccagtg gtaaagcaaa gcgatcgcca ccactctcaa tttttggtga
    15181 agattcaaag gtcagacctg aaatattgcc caaacgccgt agcattcttt tcaaatcagg
    15241 ctgttcagac agaggaactc ccggtaatcc aatcgctaaa tgccaaatgt tcgttacttt
    15301 attttgtgca cctaggcgta agccccgccc tcgtagttgt tgtgtttgca caaaagtgga
    15361 agcttgatta gccaagatca acagattaat tgcaggacaa tcccagcctt ctcctagcaa
    15421 actaacggtt cctaccaaaa tattcaactt cttttcggtt aataggcgag taactgcttg
    15481 catcaatgct tggcggtttt tgtcatccac cgtcaaataa aaataacccg acatataagg
    15541 atgttcattc atacccgtcg ctagttctgc cagctcaaca gttactagca ttttcggtaa
    15601 cagtaagaac tctccacaga tggcaccgat ttgctgagtg ttacctaata gacgaacaac
    15661 ttcatcaaac aaaggaacca cgccataagc taaggtacca tcactgcctg ctaatacctc
    15721 cttgcaaata cgatccatca aaatgaccgc tgtcagttct tgcttcagct gaacttcttc
    15781 ttttaacaaa atttgacaaa ctgcttgctt tttctcagga atcgcctgac taccaacagt
    15841 gtctggatac tctggaaaca aatgcagcga ctcatcaaac aatcatccag cttttttcaa
    15901 ttgataaaaa ataggcaatt ccggttgatc ttcaaacatc tgctgataaa tagtgatcag
    15961 ctctttttta cctaaagatg gaactgaagc ttttttacct tgctgcttag aactgcctaa
    16021 tagctgccaa tgttcgggta tcaattcata gttatgatga ttcaagtaaa ttaatgcact
    16081 agtgaacagc tcaaaatttt cataaatttc cttcgaatgc tcaagcggat gctgaacaaa
    16141 ttctaaagaa agcaaataat ccaccgtctc ttggctttcc attaacgctt ccaccagctc
    16201 attttgtttc tctaaaaagg taaaataatg ctgagtagtt tcctgattag ccggaatcaa
    16261 atatagaaaa tcctgatgag gacatagaac ctcctccttt accaactcgg gaatgctaat
    16321 ttcttcgtca atcaggccat tttaactcaa tatattgctt ccactccgtc tgagagctat
    16381 caatcggagg cgtggctgtc aaggaaatcg tcaacagatt cggttgtttt ttcttcaagt
    16441 caatcaacaa ttcaccccag ctttttttta aatgatgagc ttcatcaaaa aactaacagc
    16501 tccagctgat caccataagt tggctcctcc ttcagtcggt tgtaaatggt ctgatacgtt
    16561 tccactgtaa tatgactagg atgttcaatc gccccagaaa gatagcaact atctgctgga
    16621 tttactaaga aatattcctc gattgttgtt aaccactgtt tcttcaatat tagggaagga
    16681 accaaaatta aggttttaca atccaaacgt cggatcatct caattcctaa aaaggtcttg
    16741 ccagcccctg gtggagccac tagatggact gaattgttat ttaaataagt agcaaagtta
    16801 gtcaatatac gcttctgata gctacgccac tgaatttttt catcagatag ccacatccca
    16861 ggtccccctc tcacgtattt tctctatcat aatctaaatg aatacagcaa aaaaaagatt
    16921 tcttcaagaa tatgtgtttt tatagtaata ttttacttac tctaaactca aatgaaaagg
    16981 taaaaaaact ggtagtacaa tcttttaaat tctcattgtt ttgtacgttc tttactatca
    17041 aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt tttagctcta taatttctaa
    17101 gactgataga aaatcattct gtttttgaag
//
LOCUS       DAJSVP010000051        16665 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSVP010000051 DAJSVP010000000
VERSION     DAJSVP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.053"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..701)
                     /gene="plsY"
                     /locus_tag="ON449_001736"
     CDS             complement(63..701)
                     /gene="plsY"
                     /locus_tag="ON449_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3934320.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            828..1262
                     /locus_tag="ON449_001737"
     CDS             828..1262
                     /locus_tag="ON449_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3934321.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1428..3473
                     /gene="parE"
                     /locus_tag="ON449_001738"
     CDS             1428..3473
                     /gene="parE"
                     /locus_tag="ON449_001738"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3934322.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3485..5935
                     /gene="parC"
                     /locus_tag="ON449_001739"
     CDS             3485..5935
                     /gene="parC"
                     /locus_tag="ON449_001739"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3934323.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6212..8443
                     /gene="pflB"
                     /locus_tag="ON449_001740"
     CDS             6212..8443
                     /gene="pflB"
                     /locus_tag="ON449_001740"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3934324.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8687..9448
                     /gene="pflA"
                     /locus_tag="ON449_001741"
     CDS             8687..9448
                     /gene="pflA"
                     /locus_tag="ON449_001741"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3934325.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9533..10459
                     /locus_tag="ON449_001742"
     CDS             9533..10459
                     /locus_tag="ON449_001742"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3934326.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10641..11534
                     /locus_tag="ON449_001743"
     CDS             10641..11534
                     /locus_tag="ON449_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3934327.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11668..11793
                     /locus_tag="ON449_001744"
     CDS             11668..11793
                     /locus_tag="ON449_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934328.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11927..12559)
                     /locus_tag="ON449_001745"
     CDS             complement(11927..12559)
                     /locus_tag="ON449_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934329.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12707..13249)
                     /locus_tag="ON449_001746"
     CDS             complement(12707..13249)
                     /locus_tag="ON449_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3934330.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13384..13773
                     /locus_tag="ON449_001747"
     CDS             13384..13773
                     /locus_tag="ON449_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934331.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13777..14541
                     /locus_tag="ON449_001748"
     CDS             13777..14541
                     /locus_tag="ON449_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3934332.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14670..15617
                     /locus_tag="ON449_001749"
     CDS             14670..15617
                     /locus_tag="ON449_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3934333.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15747..16487
                     /locus_tag="ON449_001750"
     CDS             15747..16487
                     /locus_tag="ON449_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934334.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5885 a         2818 c         3460 g         4502 t
ORIGIN      
        1 aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg aatgaaaaag
       61 tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc agtatggttt
      121 ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac taaaaaggca
      181 atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata aggaaggacg
      241 atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac catacttgta
      301 caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg attaaatcct
      361 aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc aaaaatcgga
      421 aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc agaaaggtgg
      481 aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag caggacaaca
      541 atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt accgctcccg
      601 tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga aggaatagaa
      661 cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc tctttcaagt
      721 tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa aaatgaaaga
      781 tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa
      841 atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag
      901 gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg caagacaatg
      961 gatataaaat cataccagta aatccattat tggctggtga agaaattcta ggcgagaaag
     1021 tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta tttagacgga
     1081 gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg
     1141 cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca ggacgtacag
     1201 atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa aaccaaaaat
     1261 aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag cggttaggag
     1321 ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc tagtatgata
     1381 atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag
     1441 taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag gcagttagaa
     1501 aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat ttagtctatg
     1561 aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag attgatgtga
     1621 ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg cctgtaggaa
     1681 tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat gctggcggaa
     1741 agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga gccagtgtgg
     1801 taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta gagtatcagc
     1861 agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga aaaacaaaaa
     1921 aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt acaacgaaat
     1981 tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta aaaggcgtta
     2041 aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt cattacgaag
     2101 aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg acaccagttg
     2161 tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg
     2221 gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat gggggaaccc
     2281 atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg agaaaagttg
     2341 gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa gggttagctg
     2401 ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa
     2461 aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa caaatgggct
     2521 tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca atcaaagcca
     2581 gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta
     2641 aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga aaccctaaga
     2701 aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa caaggaagag
     2761 acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc gaaaaagcta
     2821 aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg attggtgcag
     2881 gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc atcatgaccg
     2941 atgcggatac agacggcgcc catattcaag tattactgct tactttcttt tatcgttata
     3001 tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta tataaagtat
     3061 ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac gaattggcag
     3121 caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga cttggagaga
     3181 tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg ttgatccgcg
     3241 tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg ggtgacaaag
     3301 tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca
     3361 gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat gatttattag
     3421 atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa taggaggtca
     3481 cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga tgggtgaccg
     3541 cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata tccgtgatgg
     3601 attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca atacgtatga
     3661 caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt atcatcctca
     3721 tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga agttgcggga
     3781 agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac ctgctgcgat
     3841 gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag atatcgagaa
     3901 agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa cagtccttcc
     3961 agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg gatatgcaac
     4021 agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata tgatcgatca
     4081 tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact ttccaacagg
     4141 agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt
     4201 tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc aaatcgtgat
     4261 cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg atgagatccg
     4321 tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata gaacaggtct
     4381 gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga attatttgtt
     4441 caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg accatatgac
     4501 tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc gaaagcaagt
     4561 cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc atatcgtaga
     4621 aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa
     4681 agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag aagaacaagc
     4741 agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca cagagcttca
     4801 aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc tttcaaatga
     4861 caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc
     4921 cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata cgcaagtctt
     4981 agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag
     5041 ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag gcgactactt
     5101 gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa ataaagcaaa
     5161 tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg ctggtgagca
     5221 catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg ttttcccata
     5281 tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat tgatcaagca
     5341 aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc tcagctgtat
     5401 gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag atcagccagg
     5461 cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt taacagatgt
     5521 tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag atggagatag
     5581 gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa tcgttactca
     5641 gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac gtgcgaagcg
     5701 gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct atatgtcgga
     5761 aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc
     5821 aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg atgagaaaaa
     5881 aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt
     5941 gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc tttttcattc
     6001 atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat
     6061 gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa
     6121 aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt
     6181 acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac
     6241 caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg
     6301 cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt
     6361 gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat
     6421 cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt
     6481 cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat
     6541 ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt
     6601 cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg
     6661 tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat
     6721 catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa
     6781 aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga
     6841 agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg
     6901 ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg
     6961 atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc
     7021 attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc
     7081 acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc
     7141 tgaatataac caattattct caggattccc tatctgggct actttatcca ttgcaggtat
     7201 gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac
     7261 aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga
     7321 aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa
     7381 tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc
     7441 aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac
     7501 actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga
     7561 atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa
     7621 tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc attatatgca
     7681 tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac
     7741 atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata
     7801 tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg
     7861 cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt
     7921 ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac
     7981 ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga
     8041 tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca
     8101 aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga
     8161 tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag
     8221 aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa
     8281 tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc
     8341 gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca
     8401 acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag
     8461 tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag
     8521 tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa
     8581 ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca
     8641 gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac
     8701 aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt
     8761 tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg
     8821 gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt
     8881 cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca
     8941 gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt
     9001 ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt
     9061 gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg aagagcataa
     9121 aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat
     9181 caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata
     9241 tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct
     9301 tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga
     9361 agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga
     9421 agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct
     9481 ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa
     9541 aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt
     9601 ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa
     9661 tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc
     9721 agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat
     9781 ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt
     9841 tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat
     9901 tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct
     9961 ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga
    10021 agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga
    10081 aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga
    10141 tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga
    10201 ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa
    10261 agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga
    10321 attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac
    10381 caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt
    10441 gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg
    10501 attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag
    10561 tcaattatga ctcttgttct tcctcctttt tacgtaaaag ccttttaagt aaaataaaac
    10621 ggatagaaaa gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa
    10681 aactgaccga tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac
    10741 aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc
    10801 ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact
    10861 ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag
    10921 ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt
    10981 gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc
    11041 gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt
    11101 cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc
    11161 ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt
    11221 acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc
    11281 gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat
    11341 cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat
    11401 tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc
    11461 tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga acagttttag
    11521 tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt
    11581 tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc acctattaga
    11641 ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca
    11701 aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag
    11761 tatggcattt tatgggaaga actagctgat tagattagga agtccatttg ataatccaga
    11821 atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta
    11881 gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat
    11941 gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga
    12001 gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag
    12061 caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga
    12121 agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata
    12181 aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa
    12241 aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta
    12301 acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc
    12361 aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat
    12421 ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac
    12481 atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg
    12541 ttctacgttg aatttccata atatctccaa attaaattga taagtatata aagtataata
    12601 acaaattgat gagtatatgt cgatgttttt taatcaaaat aataaataaa aactgtctaa
    12661 catttaaaat ttaatttaga tattagacag tttttaagtt tgtgagttat tttccttgtc
    12721 gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga
    12781 taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag
    12841 gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca
    12901 caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg
    12961 gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga
    13021 cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac
    13081 gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg
    13141 gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat
    13201 aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc
    13261 ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt
    13321 atacatacaa aaaaatgata aaatgattat aatagaatta taaagatagg ttaggtgatt
    13381 aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg tgctaagtct ccttctcagt
    13441 atatacttag gtgtctattc gattcaacag ccaattgagg tagagaacat tgtttttcag
    13501 atgtcgctga cagatggttt aattttaagc gtttttttct ttttgatact gtttattggt
    13561 aatattttcg gtagcgttgc cagttttttg aatttttcta tttatccact cctttcgcta
    13621 gcattaggag tggtgggact ttgttctctg tgcttatttc ccgagccatt cagtcctgca
    13681 tttatattat ttggtacact gaacctgttc cagacaactg ttggagcatg gttgctatgg
    13741 cgatcaggaa atttaatgaa aataggtgag taacaattga atccaattat attgaaacgt
    13801 tctgtccatc atattccagt gttagaagtt gttctgaaag aattaagaga ggaacgtatt
    13861 cccgttgtca tctattatca cggatggcag acaagtaagg agttagtttt gacgcaagga
    13921 agaaaaattg cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa tcatggggag
    13981 cgcaaacagc cggtttcttc tattccatcc tatacatttt gggatagtat ctacggaaat
    14041 ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa gaggtcttct acatgatcag
    14101 cttgcagttg gcggggtttc gatgggggga atgacaacat gcgccttgtt ggctaaacat
    14161 cctcatatag tagcgggcgt gtgtttgatg ggttcccctg cacctttgga atatggaaaa
    14221 gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac caaaagacta ttttgatttg
    14281 atttcatggg ttaagagcta tgatttgtca ttgcagccgg aaaagttagc tggacgtcct
    14341 ctgttctttt ggcatggaca agctgacgaa aaaatccctt atatacaaac ggaacgattt
    14401 gtagaaaaaa atcggcataa gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca
    14461 cagcgacata tggtacaaat accactaatg gaagaagctt ccactttttt agcaaaaaat
    14521 ttgataaaaa agtataaata attgaaacgg aaaatcactc atgacgatag aggattttcc
    14581 gttttttatt ttgggctata ataaactctg ttaggaaaat ttggtataat tgatagtaag
    14641 aatgacaaca tgttagaaag gaagagagca tgcccacttt agcagatgtt gcaaaacgtg
    14701 ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca tccggaacag gtgactgacg
    14761 aattgaaaga gttagttttc tcagcaatgg ctgaactgga ttaccgtcca aatattgctg
    14821 caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt atttatactg gaagaaatcg
    14881 acacaactga gccctattac atgaatttgc tgatgggaat cgctaaacgg atcggtaaag
    14941 cgcattattc tctccaatta gttacaaatg atgccttcga cgtcggttct tgtgatggat
    15001 acatcatcac aggtgtcaga aaaaaagact tcgattggat cgaccgtttg gaaaaaccag
    15061 ttgtcttgtt tggggaaaat tctgaaggat atgattatgt agacagcgat aatgaatatg
    15121 gtacagcaaa agcaacgcaa tatgccttag aaagagatta tcaaacgatt atttatatcg
    15181 gtatcgaagt ggacgagcct tttgaaattt caagggaaac tggttattta tctgtgatga
    15241 aggaagttga tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa
    15301 gatatatgac agaaaactgg gcagatttta cagataaaac ttgttttgta tgtagttcag
    15361 atcggctagc actaggagca gtaagaggga ttataaatgc tggaggacac ttgcccgaag
    15421 aatttggtgt gattggattt gatggggtat ttttagacca aatcagttca cctaaattga
    15481 caacgatcaa gcaagatatt atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa
    15541 aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca
    15601 gagagacaac gaattaagtc ttattactat atatatattt atgaaattca tcgctatttt
    15661 cttaaaataa tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag
    15721 ttccattttt gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc
    15781 ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg
    15841 acggacaaga agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat
    15901 ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat
    15961 tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata
    16021 ccggccatta ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct
    16081 ctcgttctgt cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat
    16141 ctgttgacac gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga
    16201 ttatcccaga ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa
    16261 aaacggatga ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa
    16321 atggtgagcc gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt
    16381 cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga
    16441 tcgatgatca aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat
    16501 ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc
    16561 cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata
    16621 agtaagaaaa taatggcaaa ataaaaaaac aaactatttt cgcag
//
LOCUS       DAJSVP010000052        16295 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSVP010000052 DAJSVP010000000
VERSION     DAJSVP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.054"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            78..1505
                     /locus_tag="ON449_001751"
     CDS             78..1505
                     /locus_tag="ON449_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934335.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1597..1962)
                     /locus_tag="ON449_001752"
     CDS             complement(1597..1962)
                     /locus_tag="ON449_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3934336.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1959..2324)
                     /locus_tag="ON449_001753"
     CDS             complement(1959..2324)
                     /locus_tag="ON449_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3934337.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2516..2764)
                     /locus_tag="ON449_001754"
     CDS             complement(2516..2764)
                     /locus_tag="ON449_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934338.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2763..2891
                     /locus_tag="ON449_001755"
     CDS             2763..2891
                     /locus_tag="ON449_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR3934339.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2948..4642
                     /locus_tag="ON449_001756"
     CDS             2948..4642
                     /locus_tag="ON449_001756"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR3934340.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4646..4765
                     /locus_tag="ON449_001757"
                     /pseudo
     CDS             4646..4765
                     /locus_tag="ON449_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5293..6426
                     /locus_tag="ON449_001758"
     CDS             5293..6426
                     /locus_tag="ON449_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR3934341.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6457..6777
                     /locus_tag="ON449_001759"
     CDS             6457..6777
                     /locus_tag="ON449_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR3934342.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6806..7270)
                     /locus_tag="ON449_001760"
     CDS             complement(6806..7270)
                     /locus_tag="ON449_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934343.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7402..8655)
                     /locus_tag="ON449_001761"
     CDS             complement(7402..8655)
                     /locus_tag="ON449_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934344.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8684..9001)
                     /locus_tag="ON449_001762"
     CDS             complement(8684..9001)
                     /locus_tag="ON449_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3934345.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9027..9347)
                     /locus_tag="ON449_001763"
     CDS             complement(9027..9347)
                     /locus_tag="ON449_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934346.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9811..11226)
                     /locus_tag="ON449_001764"
     CDS             complement(9811..11226)
                     /locus_tag="ON449_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3934347.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11243..12559)
                     /locus_tag="ON449_001765"
     CDS             complement(11243..12559)
                     /locus_tag="ON449_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934348.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12709..13407)
                     /locus_tag="ON449_001766"
     CDS             complement(12709..13407)
                     /locus_tag="ON449_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3934349.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13696..14651)
                     /locus_tag="ON449_001767"
                     /pseudo
     CDS             complement(13696..14651)
                     /locus_tag="ON449_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14764..15597)
                     /gene="msrA"
                     /locus_tag="ON449_001768"
     CDS             complement(14764..15597)
                     /gene="msrA"
                     /locus_tag="ON449_001768"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3934350.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15772..>16295)
                     /locus_tag="ON449_001769"
     CDS             complement(15772..>16295)
                     /locus_tag="ON449_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934351.1"
                     /translation="YLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTK
                     GMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITL
                     PVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTA
                     YLCLFFKIVFHKR"
BASE COUNT         5084 a         3280 c         2766 g         5165 t
ORIGIN      
        1 aactgatgat ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac
       61 aaggcggagg ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt
      121 ttttatgtgg gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc
      181 tgcatgtttg caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag
      241 aacagatcgc ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata
      301 tacctcgatg caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc
      361 tgctttattt ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct
      421 ctgtcagcaa agcttattca ttaaaaaaac aagtattagc ttactttaat gactgcggga
      481 tcgaaatcga ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta
      541 atgtgtcgta tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac
      601 gtgccgatcg tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa
      661 ataaagaaat cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg
      721 tgacaataga ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg
      781 aatcagcatg ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta
      841 ttttgacact attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag
      901 actttcaaca gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg
      961 ctacctttga atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc
     1021 tgattcatct gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt
     1081 atcttttaaa tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct
     1141 ggtccagcca gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga
     1201 tcgaattgag cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa
     1261 attcggatgt tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt
     1321 ttcaagttgc accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt
     1381 cctcaaaccg ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa
     1441 atattattcc tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca
     1501 aataaggggc tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc
     1561 tgtaacaatc tcttgataca cgtcaggctc acaacctcac ttattttgga catctagggg
     1621 tccgatctct ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag
     1681 ttggaaaata tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag
     1741 aatttccaaa cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct
     1801 tactcggtcg ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga
     1861 tagacaaaca ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt
     1921 ttcgaaatat ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt
     1981 tgtttttctt tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata
     2041 ataataagtg caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata
     2101 tcagcaacga ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta
     2161 tctgcattac gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt
     2221 ttgaatagaa caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata
     2281 gcaatgatcc attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt
     2341 cttttaattt gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa
     2401 agttgttcta atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc
     2461 ctccatccat aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt
     2521 ccgtcatcag cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa
     2581 aaatagaacc atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag
     2641 gaaaatcaga cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt
     2701 ggcattctaa taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt
     2761 tcatgagtaa aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg
     2821 catatctagg aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg
     2881 aatcaatcta gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg
     2941 aatcacaatg agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc
     3001 ggctgtttat ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt
     3061 ggatgatcac ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa
     3121 taaaaaccat ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa
     3181 ttaccgtacc tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc
     3241 agggatgact gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa
     3301 agcacgtttg atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa
     3361 taaagaccgt attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact
     3421 agacaatatc actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt
     3481 ctggtttatg tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg
     3541 tcgctatatg catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa
     3601 tcgaacacgt tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga
     3661 acaaggatgc aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc
     3721 gatgcaagat gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga
     3781 agaatacgtt gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga
     3841 ttcagctact ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc
     3901 ttctagtctc tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt
     3961 ctttgctgat cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt
     4021 gttgttgaat gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt
     4081 tatttcaacc acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt
     4141 gcatcatcaa ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt
     4201 cctttatcca agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa
     4261 agaaatgcta gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat
     4321 tcgtgaaaaa gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc
     4381 ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa
     4441 acattcgacc aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt
     4501 tactgaacaa agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc
     4561 tatgtcccgt cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc
     4621 cgcaaaaaaa atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     4681 aagaacaacc aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca
     4741 gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt
     4801 aataagagca gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc
     4861 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg
     4921 agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat
     4981 accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa
     5041 aacaactatt ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac
     5101 tatttcaatg attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac
     5161 ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat
     5221 ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa
     5281 ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac
     5341 gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc
     5401 atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat
     5461 gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg
     5521 aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc
     5581 ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt
     5641 ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt
     5701 ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt
     5761 ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa
     5821 aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc
     5881 gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat
     5941 aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga
     6001 acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg
     6061 gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca
     6121 atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact
     6181 ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc
     6241 gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt
     6301 gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca
     6361 gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa
     6421 ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc
     6481 catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc
     6541 tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc
     6601 tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt
     6661 tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt
     6721 tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa
     6781 tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg
     6841 tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg
     6901 tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga
     6961 tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg
     7021 cacaaagatt tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc
     7081 ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat
     7141 tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg
     7201 aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat
     7261 atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct
     7321 ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact
     7381 tgttctccgt tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt
     7441 agctaccttg acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag
     7501 tacaccacct gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca
     7561 tggaggcata acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact
     7621 aactaagacc attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg
     7681 taacccaaac atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt
     7741 tacaaccata tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa
     7801 agttgcacct gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa
     7861 cggtgcgcta tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat
     7921 tgtttgcatc aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg
     7981 tgtgataaat gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc
     8041 ttggaccact gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc
     8101 agcaactaaa gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt
     8161 tgcaactgcc ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa
     8221 acgtacaaag atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga
     8281 gctcatacct aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga
     8341 aacaccgtct tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac
     8401 agaaagaatg gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc
     8461 accaaaaatt gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac
     8521 tgctaatgct cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac
     8581 taaatgccgt tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc
     8641 aacaaattta tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac
     8701 gatacagatc aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat
     8761 cttgactgtg gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca
     8821 gcatctctgt ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt
     8881 catctgctaa agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt
     8941 ttgcatttcc gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca
     9001 tttattgtat cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt
     9061 actttttcac cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct
     9121 ttaggtgcta gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt
     9181 aaaacgtcta catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag
     9241 atgtctgctt ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt
     9301 gtactcattc cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc
     9361 ctttctatac ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa
     9421 aagttgatat catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc
     9481 gcacactaaa cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa
     9541 gttgtgacac aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc
     9601 tgcggtaata agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt
     9661 attacttgat gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga
     9721 ccctgggtat tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa
     9781 atacaatgaa catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa
     9841 ttctctctta tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg
     9901 ttcatcgttc aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga
     9961 cacacagtca gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc
    10021 tttgatcaag taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat
    10081 tcctttctca tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag
    10141 ccaagggata ttatgataat tttcttttag atacatggct atatcataca tgattttcgg
    10201 atagatttcc catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata
    10261 atatcgttca ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg
    10321 tttaggataa tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa
    10381 atcctctgtt gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc
    10441 tgtgtagtat ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat
    10501 ataagcagct ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa
    10561 aatacagcca atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt
    10621 tgctaaaact ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca
    10681 gcgaacagca tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca
    10741 gttttttacc ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc
    10801 aactactttt cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata
    10861 atgttccaaa caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact
    10921 tatatgctga gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc
    10981 ccaattgata gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg
    11041 ctggtattgt tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa
    11101 atgttcattt ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca
    11161 accttccgtt tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat
    11221 tttcatgatt tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta
    11281 caaatggcag atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac
    11341 cgatatttcc tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca
    11401 ttacaactgc tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga
    11461 taagaggcgt taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa
    11521 aaataactgg ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt
    11581 tcatatcttc ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag
    11641 acccgcccat actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt
    11701 ttccagcttc aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta
    11761 tcggcttcac catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca
    11821 tcaatagcaa aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat
    11881 tgtaaatgat atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg
    11941 cgatacccga tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg
    12001 ttggcacggt atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa
    12061 gttcaataga aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt
    12121 gtttagccaa tacgttttct actataacag tctgattttc attcactaat tcataaacag
    12181 ttggattaac agataaataa gacatcactg cgatgatacc agggaataca ccatccactt
    12241 tattcagctt ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta
    12301 gtgtgacata gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac
    12361 ttatctgtgc taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag
    12421 cacccgtaat aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc
    12481 ttaaattatt gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac
    12541 tcattccaga tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta
    12601 gtcagcgctt acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa
    12661 acaaaaattc atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc
    12721 tttttctcct attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc
    12781 atcaaacgca gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc
    12841 gatcgtctcg attttcagat gctggatatc ataattttta ttaaacaaag caattgattc
    12901 attaatgatt gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt
    12961 atgaaaatcg atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat
    13021 cttttgcgtt aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa
    13081 acctgccatc ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc
    13141 ctgatcttca atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac
    13201 aaatgtcccc gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc
    13261 ttctcgcacc gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag
    13321 tttatctcct acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac
    13381 ttctactagg gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt
    13441 tcgtaattat tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa
    13501 tccgttcata gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt
    13561 cactcctagc cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag
    13621 acgatcttca ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt
    13681 ttgattacat cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa
    13741 atctaaatta tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac
    13801 cagtacactt gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga
    13861 ttgtcctcgt gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac
    13921 ttgttcttct gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc
    13981 atgtagataa cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag
    14041 cccttcttct tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt
    14101 gtagcgcatc tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt
    14161 tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca
    14221 agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga
    14281 tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg
    14341 atggatttac gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg
    14401 ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag
    14461 tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt
    14521 cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca
    14581 aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta
    14641 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
    14701 caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag
    14761 gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata
    14821 gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta
    14881 aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac
    14941 gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc
    15001 tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa
    15061 ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata
    15121 acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc
    15181 acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga
    15241 taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa
    15301 tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg
    15361 ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt
    15421 cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga
    15481 tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt
    15541 attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga
    15601 ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga
    15661 ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcctt
    15721 atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa tttacctttt
    15781 atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa gataagaaat
    15841 aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt tgactccgct
    15901 tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat ttatttctac
    15961 attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct cattagttac
    16021 tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt gactgccaat
    16081 ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag gagaataatc
    16141 atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt tgtttacaac
    16201 aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa
    16261 agtgattaat aacaaaacag tcataaataa ataaa
//
LOCUS       DAJSVP010000053        15938 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSVP010000053 DAJSVP010000000
VERSION     DAJSVP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.055"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(173..475)
                     /locus_tag="ON449_001770"
     CDS             complement(173..475)
                     /locus_tag="ON449_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934352.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="ON449_001771"
     tmRNA           complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="ON449_001771"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1232..2005)
                     /locus_tag="ON449_001772"
     CDS             complement(1232..2005)
                     /locus_tag="ON449_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934353.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2149..2499
                     /locus_tag="ON449_001773"
     CDS             2149..2499
                     /locus_tag="ON449_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3934354.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2480..3196
                     /locus_tag="ON449_001774"
     CDS             2480..3196
                     /locus_tag="ON449_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934355.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3196..4644
                     /locus_tag="ON449_001775"
     CDS             3196..4644
                     /locus_tag="ON449_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3934356.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4714..5223)
                     /locus_tag="ON449_001776"
     CDS             complement(4714..5223)
                     /locus_tag="ON449_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934357.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5213..5938)
                     /locus_tag="ON449_001777"
     CDS             complement(5213..5938)
                     /locus_tag="ON449_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934358.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6161..8281)
                     /locus_tag="ON449_001778"
     CDS             complement(6161..8281)
                     /locus_tag="ON449_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3934359.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8511..9689
                     /locus_tag="ON449_001779"
     CDS             8511..9689
                     /locus_tag="ON449_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3934360.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9705..10463
                     /gene="aroD"
                     /locus_tag="ON449_001780"
     CDS             9705..10463
                     /gene="aroD"
                     /locus_tag="ON449_001780"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3934361.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10486..10971
                     /gene="ybaK"
                     /locus_tag="ON449_001781"
     CDS             10486..10971
                     /gene="ybaK"
                     /locus_tag="ON449_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3934362.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11042..12295)
                     /locus_tag="ON449_001782"
     CDS             complement(11042..12295)
                     /locus_tag="ON449_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3934363.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12412..13761
                     /gene="gdhA"
                     /locus_tag="ON449_001783"
     CDS             12412..13761
                     /gene="gdhA"
                     /locus_tag="ON449_001783"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3934364.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13873..15219
                     /locus_tag="ON449_001784"
     CDS             13873..15219
                     /locus_tag="ON449_001784"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3934365.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15527..15913)
                     /locus_tag="ON449_001785"
     CDS             complement(15527..15913)
                     /locus_tag="ON449_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3934366.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5216 a         2844 c         3192 g         4686 t
ORIGIN      
        1 cttataattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
       61 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt
      121 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
      181 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      241 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      301 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      361 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      421 atctattcga aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt
      481 tcacctcaaa aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata
      541 atatggatgg aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt
      601 ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg
      661 atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc
      721 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      781 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      841 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      901 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      961 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
     1021 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
     1081 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
     1141 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
     1201 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
     1261 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
     1321 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
     1381 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1441 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1501 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1561 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1621 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1681 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1741 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1801 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1861 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1921 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1981 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     2041 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     2101 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     2161 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     2221 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     2281 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     2341 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     2401 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2461 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2521 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2581 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2641 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2701 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2761 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2821 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga
     2881 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2941 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     3001 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     3061 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     3121 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     3181 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc
     3241 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     3301 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     3361 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3421 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3481 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3541 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3601 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3661 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3721 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3781 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3841 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3901 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3961 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     4021 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     4081 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     4141 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     4201 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     4261 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     4321 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     4381 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4441 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4501 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4561 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4621 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4681 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4741 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc
     4801 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4861 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4921 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4981 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     5041 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     5101 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     5161 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     5221 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     5281 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     5341 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     5401 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5461 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5521 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5581 ggaataattt ggttttagaa agaccagtca ggtgccaatt gatcaattcc tgcttgtttt
     5641 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5701 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5761 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5821 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5881 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5941 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     6001 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     6061 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     6121 cgacaataat tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg
     6181 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
     6241 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     6301 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
     6361 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     6421 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     6481 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     6541 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     6601 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     6661 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     6721 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     6781 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     6841 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     6901 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     6961 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     7021 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     7081 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     7141 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     7201 cagcgttccc atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg
     7261 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     7321 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     7381 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     7441 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     7501 tttctaaatg gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg
     7561 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     7621 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     7681 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     7741 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     7801 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     7861 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     7921 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     7981 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     8041 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     8101 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     8161 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     8221 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     8281 agataatctt cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag
     8341 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     8401 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     8461 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     8521 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     8581 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     8641 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     8701 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     8761 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     8821 gagacgggat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     8881 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     8941 cggaaatcat aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     9001 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     9061 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     9121 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     9181 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     9241 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     9301 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     9361 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     9421 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     9481 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     9541 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     9601 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     9661 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg
     9721 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     9781 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     9841 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     9901 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     9961 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
    10021 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
    10081 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
    10141 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
    10201 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
    10261 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
    10321 gtaaaatttc tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa
    10381 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
    10441 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
    10501 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
    10561 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
    10621 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
    10681 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
    10741 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
    10801 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
    10861 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
    10921 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
    10981 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
    11041 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
    11101 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
    11161 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
    11221 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
    11281 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
    11341 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
    11401 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
    11461 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
    11521 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
    11581 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
    11641 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
    11701 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
    11761 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
    11821 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
    11881 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
    11941 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
    12001 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
    12061 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
    12121 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
    12181 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
    12241 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
    12301 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca
    12361 aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac
    12421 gcaacagaat atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa
    12481 gaatttttac aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat
    12541 cccgaatata tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa
    12601 tttcgagtgc cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc
    12661 caatacaact cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat
    12721 ctaagtatct tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg
    12781 ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag
    12841 atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt
    12901 gatgttcctg cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa
    12961 tataaacgat tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg
    13021 ggaagtaaga tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta
    13081 ttgaacgaag aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat
    13141 gtagccattt atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca
    13201 gattctagcg ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc
    13261 aaagaaatca atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat
    13321 catgctggag aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct
    13381 actcaaaatg aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc
    13441 gtagcagaag gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca
    13501 ggcgtgtggt attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta
    13561 gaaatgagtc agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg
    13621 gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac
    13681 gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg
    13741 gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt
    13801 tatttgcttg aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa
    13861 ggaggagaac aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac
    13921 gaacaagagt tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa
    13981 ggtactggcg caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa
    14041 gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta
    14101 gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat
    14161 cacacattct tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc
    14221 ggaaattcaa tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac
    14281 ttttcagtaa acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt
    14341 gtattcaaag agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac
    14401 gctacaacag ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa
    14461 actttcgtga ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg
    14521 ttaccaatcg ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca
    14581 agcaaagcat actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg
    14641 cgcaacattc tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga
    14701 atgcaatact tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa
    14761 aaaggcatct atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc
    14821 atccaagaag gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa
    14881 tcaatcacaa ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa
    14941 gaagtggact tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt
    15001 gacgtgccta acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca
    15061 atgtacttct ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc
    15121 gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc
    15181 tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat
    15241 ttaaataatc agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt
    15301 actcaaacat actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag
    15361 tcccttgttt gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta
    15421 agaacagtga ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag
    15481 caaaaaagca ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta
    15541 atttttcttt tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc
    15601 gcttttcgct tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag
    15661 agtcaccaga agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag
    15721 catctacgtt gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag
    15781 tttctgcagg cattggagct ttgactaccg cataataatc tttacctaca taagtagact
    15841 gatagtagtc ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta
    15901 aaattttttt catgaaagat ctcctttttt atttttat
//
LOCUS       DAJSVP010000054        15699 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSVP010000054 DAJSVP010000000
VERSION     DAJSVP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.056"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            419..805
                     /locus_tag="ON449_001786"
     CDS             419..805
                     /locus_tag="ON449_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3934367.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            899..1090
                     /locus_tag="ON449_001787"
     CDS             899..1090
                     /locus_tag="ON449_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934368.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1604..1936
                     /locus_tag="ON449_001788"
     CDS             1604..1936
                     /locus_tag="ON449_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3934369.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2232..2387
                     /locus_tag="ON449_001789"
     CDS             2232..2387
                     /locus_tag="ON449_001789"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3934370.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2663..4003
                     /locus_tag="ON449_001790"
                     /pseudo
     CDS             2663..4003
                     /locus_tag="ON449_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4244..5860)
                     /locus_tag="ON449_001791"
     CDS             complement(4244..5860)
                     /locus_tag="ON449_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3934371.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5978..6196
                     /locus_tag="ON449_001792"
     CDS             5978..6196
                     /locus_tag="ON449_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934372.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6391..6852
                     /locus_tag="ON449_001793"
     CDS             6391..6852
                     /locus_tag="ON449_001793"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3934373.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7045..7284)
                     /locus_tag="ON449_001794"
     CDS             complement(7045..7284)
                     /locus_tag="ON449_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934374.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7308..8831)
                     /locus_tag="ON449_001795"
     CDS             complement(7308..8831)
                     /locus_tag="ON449_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3934375.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8849..8971)
                     /locus_tag="ON449_001796"
     CDS             complement(8849..8971)
                     /locus_tag="ON449_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3934376.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9001..9984)
                     /locus_tag="ON449_001797"
     CDS             complement(9001..9984)
                     /locus_tag="ON449_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3934377.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10008..10157)
                     /gene="rpmG"
                     /locus_tag="ON449_001798"
     CDS             complement(10008..10157)
                     /gene="rpmG"
                     /locus_tag="ON449_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3934378.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10178..10555)
                     /locus_tag="ON449_001799"
     CDS             complement(10178..10555)
                     /locus_tag="ON449_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934379.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10587..10766)
                     /gene="rpmF"
                     /locus_tag="ON449_001800"
     CDS             complement(10587..10766)
                     /gene="rpmF"
                     /locus_tag="ON449_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3934380.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10781..11050)
                     /gene="rpsN"
                     /locus_tag="ON449_001801"
     CDS             complement(10781..11050)
                     /gene="rpsN"
                     /locus_tag="ON449_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3934381.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11573..13339)
                     /locus_tag="ON449_001802"
     CDS             complement(11573..13339)
                     /locus_tag="ON449_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934382.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13299..15053)
                     /locus_tag="ON449_001803"
     CDS             complement(13299..15053)
                     /locus_tag="ON449_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934383.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15163..>15699)
                     /locus_tag="ON449_001804"
     CDS             complement(15163..>15699)
                     /locus_tag="ON449_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3934384.1"
                     /translation="ATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISY
                     NYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVAS
                     WALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVAS
                     LSMIGVLIGRKVLKKYKK"
BASE COUNT         5082 a         2997 c         2400 g         5220 t
ORIGIN      
        1 taaatattcc aatttgggaa tccaataaaa attttcgttc aaatacgtgt ctattcgaac
       61 tcttttctag ttgttaatta tgaaggggga agaaataaaa aatacaatct atacacgaat
      121 aagaatatta tccaaaagga ggttttagaa aagatttatt atacgtaaaa agctcaagac
      181 attttcttag ccataaggac atgtttgata tttaagaaga tatatgaatt ttcttttcta
      241 agtataaaga taactatcca ttatcaacta aaaaaacatt tttgaaaata aacatcagca
      301 gtcctatcaa tgcatgtgag cttatagaag ttattcgcag cgcagcataa ttgcattagc
      361 ttcttttcgt gttttttgcc tcttttccct ttaaatctta atggaggagg tgtgcaatat
      421 gtcagataac gacgaacgcg taaatgccca gttgattcga ttctttgaaa aggtcatttt
      481 gaacaccgct aatacttatt ataaaaaaca agaagaaatt caaaaacatg aacaacttga
      541 tcaactccca ccatatttgt ttactaaaga tactataaaa tttaaacaac cgattaatgt
      601 tttaaatgtg actctccttg ttgaagatat aaaattagca aaaatccttc ctttgttgaa
      661 agaacctgaa caaatttttc ttatcgaaaa atttatcttt gataaaactg ataaagaaat
      721 tggaaaggtt ctggggatta caagacaggg tgcgacaaat ttaaaacatc gactttacaa
      781 aaaaattcaa aaaatgctgg tttaatatct gcataataac taattttgta tgcttctttg
      841 ctcgttctcc tccttcccaa agaaatagat tataagtcca tgggaagaag gtagatttat
      901 gaattcttta aaagaagatt tcattttagc aaaggctgga aatgaagaag ctgttgaggc
      961 aattctcaaa agatttagct ctttgattca taagcaatcc tggagaactg gaaaatatga
     1021 tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga aattcgaaat
     1081 taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg
     1141 aacaataaat ttgaaactat cttctttata attataaagt atcgatttta cagcgttcta
     1201 taatgattca tagaatctaa aacgaatgat tttaattatc aacgtttttt aattcgtttt
     1261 tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt tacgtatgaa
     1321 gtgggcagga ggtcgaatcc agaactgggc tatggtactt caacgaggac ttctcctcta
     1381 gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca actatgtgtc
     1441 caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt aaaaactctt
     1501 atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc tatgatacac
     1561 ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgagaa aaacatatga
     1621 tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa ctaccaagaa
     1681 aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt cagaatatcg
     1741 tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata aagcagattt
     1801 ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt ctacgaacac
     1861 aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg aatcgaaatc
     1921 aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag aggatttcat
     1981 cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga
     2041 atcgataatt tgaacagaac caaattgatg ctgtttgggt gatggatatt acttacattc
     2101 catgtaatga cggtcgcctc tatttgtcca cctatattga cttagcaact cgtattctac
     2161 gttactatat ggtagattca cacatgaaaa aagagattgt cgtccgtcca ttacaaattt
     2221 attaaggaga attgcctacg gtaattcatt ccgatagagg tagccagcat cggtctttta
     2281 tttatcaaga gctgttgaac gaccaccaca tcacacattc agtgagccaa actgggactc
     2341 ctgttgacaa tgcagtaatt gaagcgtttc atcgatcaat caaatgagaa ttaatcgact
     2401 ccaacaaata taaaagtaaa atagagatga aggtgttaat ccaagattat ttagaagact
     2461 actacccaaa caaaagaatc catacaaaat ttatgatgac tccttgacaa tttgaagcga
     2521 agaaaataag ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt
     2581 cttttttcta ataaacgcaa acagacacct taaaatcctt tagaataaag tcgacgaaaa
     2641 ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat
     2701 tctagaccta aatcttatct ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga
     2761 aacatgtttt gtttttgatg gcacattgac ctatcaaccg gaggaatgct ttcatcgcca
     2821 ctatcaaaat gaacaaacga tcattaaata gggctggaaa aaagtatcca tcttattgaa
     2881 tgatgtcagt aactacaaga ccattctgcg gattaacaaa cagcgcttca aatgtaaaca
     2941 ctgtggaaag acttttttag cagaagattc agttagtgat cgtcgctgct ccattgctcg
     3001 cagagtcaaa caagccattc ttgagctatt aagcgaacct ctttctatgt ctttgatcgc
     3061 acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa
     3121 aaccatttct ttgagccagc tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt
     3181 gaaaaatgtc tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat
     3241 tgatattgtg gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctat
     3301 aaaagtccgt gaacaagtgc gtctagtggt ttcagatttc tatacaccct atcgcacatt
     3361 ggtgaaagag tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca
     3421 cattggtcgg gcttttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga
     3481 tcgtcggtcg aagcacttaa agaaatactg gaaactactt caaaagaatg cgtgggaatt
     3541 gaaaggtcag catcgctact ggcgtccgtc atttcgagat catctgacag aagcagagat
     3601 agtcgatcgg ctattatctt atgatgattc cttaaagcga ggctacgaag tctaccagga
     3661 ttttctttcc gttatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga
     3721 ctacaaggag ttgccagagc actaccaacc agtgttcacg accttcaaga aataccaaac
     3781 agaaatcaaa cgagcgttgc gcgttcccta ttccaacggc cctatagaat gtttgaataa
     3841 ccatatcaaa gtactaaaac gaattgccta tggctataga aactttcaaa actaccgaga
     3901 acgaatcttt ttatatcgag gaaaatattt taagaaaact aagaatacta cccaactgac
     3961 taaagccaga acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga
     4021 ttattctatt gaatttcttc accaacactc attgacgaag agccaaaata agtaatctaa
     4081 ccgactgtta actaaaccat tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc
     4141 ttctttttct tgtccacaag cgcttaacac acttaataaa gcccctgcta ttattaataa
     4201 taatatcttt ttcttattaa aaacctcctc atctatttcc agattagtaa atgtagtaat
     4261 ctgtgctttc ttttttctca atactaataa ctaacttatg gggtaaacaa atactcgttt
     4321 gaccgggttt tgagatccat ccagttctaa ccgcgatttg atcaggacta ttatcttctt
     4381 taactcgaat tcttccatct ttaatctcaa caatattata ttgtcccttc gcaggatgat
     4441 aattcacgag tttatgattg atcttatcca aatcgaagcg atccatttcc ttgccatcaa
     4501 ttcgaacgat ggcatatttg atagcatctt cagttgtttc attctccttt tctatagaga
     4561 atacaaataa gggaataaaa gaggtaataa tcaaaatgcc aataattatg acatcgccta
     4621 tcttgacacc agcttttctt aaaaatttca ttaacgatat ctggctaaac atattagact
     4681 ttttccatgg aaatggtgaa atttttcgga aattatctgc attaagagaa tcagttaaac
     4741 aaccctcaag ttgttcctta atacattgtt gatccatctt cataccaata aaaactagtt
     4801 tattaatgcg ttcaccaaac aacggatgcc aggcttcctt caattcggga tttttttgta
     4861 taatcatttg acactcaatt ttaggtaaac tagctaccca ataatttaat gattcaatac
     4921 ttttatagct acccgatgtt tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa
     4981 gaaacccttt tgctcttgta atattgccag gaaaattttt atccgtaaga tgggcaaatt
     5041 tttgtgcgtc aaggagtaac cgatttcgat agataaaact tgagatacca tattcttctg
     5101 tttcaggaat atgattttga tagccaagct ctaactcttt gaaccagccc actgaatagg
     5161 aagtctcatc tatattaaaa agatttgtat ttagaatttt cgataagtct acttcacctt
     5221 taattgtcct aattattttg gcattggact gtaacttagc aattagaccg ttaattttgt
     5281 aaatcgtctt ggttgacact aagtcactat tatttaaaag taatatgtta caacactcga
     5341 attgatcaat taacaagcca cgaatatcct gttcttcttc aactacatta ccttcagctt
     5401 ttctttcctg aatcttttca cccgtttcga aattatccca aaaacgtttt acatccacta
     5461 ctataataat ggcaccaatc tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag
     5521 ctaatataat tgcttgaata attggaattg gtttgctgat tcctgaagct tcaataacaa
     5581 tttgattcaa gtcagaaaat tgcactaatt gatctagttc aacaatcaaa tcttcgcgta
     5641 atgtacaaca aatgcatcca ttcggcatcg aaacaagttt ttctccagtt cttttaaaac
     5701 ctttcttgtc gactaatacc ccatcaacat ttacttcccc tatgtcattt acaatgacag
     5761 caatttcctt tccgtgatca ccgtacagaa tatgatttag aagtatcgtt tttcctgaac
     5821 caagatagcc agttaagatt gtaataggta tttttttcat agggaacctc tttctatacc
     5881 ttgaatttac actttaagtg caacacataa aaatgagtca taatcgatat ccaactagta
     5941 taagtttgtc gagaacactc ctagaaggaa aataattatg agccagtatc gtcatcttac
     6001 tatactcgaa cgagaaagaa tctttctgct tcacgaagaa ggattttcta ttagtataat
     6061 tggcaacgac attcagcata gcccgtttac tattttacat gaactgagaa gaaacaagaa
     6121 aaaaactatt caccttccaa agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga
     6181 cgttatcaca ttctaaatga tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat
     6241 tttcagtggt cgccagagga aatctcgaat cgtttgaagc atgaacatat ggacttttca
     6301 attagtttta tcacgatcta acgtgcaatt tgcaatgatc ttttcgatac agaacccctc
     6361 tcacacggca ataaaggcct gatccgttca ttgcaccatc gaggaaaaac aagacataca
     6421 aaaaatcatg tcgagtctcg tggaaagatt tgccttactc attcgatcca tggccgtcca
     6481 aaatccgcag atgatagaag tgattttggt cattggaaag ccgatattgt agctggcaaa
     6541 actggtggta cttgtttagt gactttgaca gacagaaaat catgcttcct tttagcagga
     6601 aagaaggtcc ccagaaagaa agccgcttat gtgagcgaga aaatgattga acttttaagc
     6661 agtattccaa atgaggcggt caaaacgatc actccagata gaggaaaaga attctccaat
     6721 catttcgcag ttacagaagc attgaataat atgccctttt attttccaga tcctcatgcc
     6781 cttggcaaca atgaacaaat taaaatacga atagactcat tcgagaatac ttgccaaaat
     6841 cgaaagagat agctacggta gataactcaa cgataaatca gatgattgaa aaactgaata
     6901 acagacctag aaagtgttta gattggagaa caccttatga agtcttcttt gataaagtgt
     6961 tgcgcttgat ttgacaattc aagacataat aaaaagcaga tcaaatccaa ataagggaaa
     7021 aatgatctac tctggtatta tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg
     7081 tctacttctt ccagtattga ccttgataaa acaacaatca ggatcccgtt ttaatactct
     7141 atgaactgtg tcaatactaa catgtaaagt tgcagcaata tcagctaatg tactcccatt
     7201 taatctcatt tgctttgcaa atcgataacg ccgataagca tcaatattct tggaatccat
     7261 agacaataat acttttctta ccatgtattt cctccaaaaa ttgaatttta atgagccatc
     7321 atttcttgag caatttcatg tttagacaat gaagctggat agaaagtagg ccaattatgc
     7381 aattcattat agagtttttc ttgactttct ccaccaaaga aaatatggaa atgctctgcc
     7441 ttacttggtg caattccatg atcactaaat tgaatatatt taaaacgacc agcacctgca
     7501 tcatcagttt caaaattaaa acgaacacca cggtttcctt tttcataatt caaaattttg
     7561 tatccaacat atttgtatgt gtattgatga tgttcaccgt tgactacaaa atcaatggtg
     7621 ttgtctttaa tgttgatagt atcaatatct gtcttatagc cagtagtata ataatctttg
     7681 tactcggcag ctgtcatgtc tttattgatt tttgctttgt aatcgaaaac ttcatccaaa
     7741 attccatctt ttaacaacgg ataaacagat tgccattcgc ctgtataatc actcaattcg
     7801 cgatccttca catcggcatc tttaaagtac ccattagcaa ctgtcttttc atccttcgct
     7861 tctttttctg gaactacctc ttcaccagca accatagttg ttttttctaa cgcttttaga
     7921 ttgtcttcca ttactgaaac ataatcttca ccattatcca tttgttcttt tgtaagactt
     7981 tcaagaggat tcagtacttc caattttatc ccagcttcgt tggccaaagt ttttgcaatt
     8041 ttatcattag cattcttttc aaagtaaata taattgatct tatttttttt aacatactct
     8101 tttaattcag ctaatcgacc agaagatggc tcttcttcag gacttagtcc agcaatagga
     8161 acttgtttca atccataatc caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa
     8221 cttttttgtt tcgcttcttt aagactagtg gtatagtcag catccaaccg tttcagttta
     8281 gtcaaatact tttcagcatt tgtctcaaat acttttgctt ttttcggata caatttaact
     8341 aactgatcct tgatatttga aacttcctta attgccattt tgggtgaaac ccacgtatga
     8401 ggatctagtt catgatggtg atcttcttct ccgtgatcat gatcttcttc ttcactacct
     8461 ggcaataaaa tcatgtcttt tgttccttct actacattag gcccagcttt cttccaactt
     8521 tctttagctt ttggtaccca tgattccatg ttttcgttat gatatacaaa aacatcagca
     8581 tcatgagctt ttgccatatc cttagctgag ggctcatagt catgaggttc tgatcccgct
     8641 ggaaccatta aatccacgtt cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt
     8701 ggatagaatg ttgttacaat ttctaatttt ccatctttat cattcgatac ttttgcatca
     8761 ttagtgctgg agcaagctcc caataatcct agagctgcta tcccaataag tcctacactg
     8821 attttcttca ttctttttcc ttccttaatt atgctaattt cttcatttta tttcgtttat
     8881 gttctttgat aatttttaat gccctttttc tagttttaat attcgggctt ttaagtcgtc
     8941 gatatgcact agatatatcc gttttctcca ttagaaatta cccccaatca ctacttatca
     9001 ttatttgctt tccgccagta atttatcaaa actctgtttg atcctttctt catccaacat
     9061 ttttccgatt agaataatag tggtttggtc tttttgtcga gtggtgtatt gaaaattaac
     9121 ctgttcgttt acctcttgca tggcaactat aaaatcctgc tcttgtaaag caatcaatcc
     9181 tttaaaacga tacaggctcc cttggttggt taaaatcagc cagtcaatcc attggagtaa
     9241 aaaatctttt tttatgtctt gatctgaagt taagagaatt gatttaaact catgtacttg
     9301 atggtgttga tgatagtatt tgccattata gtgattttct gatgaatcac tttcaaaaac
     9361 taagggatga ttaaattttt tcaaattata aaaatccttt ttttgaatcg tttcactatg
     9421 tgtgaacatt cgaatatcgg ataaagggtt aatacttttg atttctctct gtaatttttt
     9481 tagtgcagat aaattcaagt tttgttttct agacacaaaa atgcgatctg ccatgacaag
     9541 ttgcttaagg gcttgtggtt ctctttcaat aagttcaaaa ctgtcactat caataacagt
     9601 caataaacta tcgatatata ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat
     9661 gattggttgg ggatctgcta tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg
     9721 atgttcttct attgtttcca gtaaagaaat caatattgct aataaatcac tgcgaagact
     9781 gcaacacata catccattat ttagttgtaa cacccgttcc tcgctatgaa ctaataattt
     9841 gtgatcaatc ccaacttcac caaactcatt ttcaattata ataatttctt ccctggggta
     9901 gggtgagact tgcaaggctt gattgattag cgttgttttt ccagccccta aaaacccaga
     9961 aacgattgta actggtatgc ccattttgtc acctttttac cacttagcta tttcacttct
    10021 ttaaaaattg cccgtctgcg caattttggc gaatactttt ttaattctaa ccgttcaggg
    10081 ttattccgtt tatttttgct ggtgagatac agacgttctc cagtttcgac acattccaaa
    10141 ataatatttt gacgcataaa tcttcctcct tcaatactta tcgatcttca agatatgctt
    10201 gaatttgttg tgcatcttca aaagttaact cgccagattc caattgtgaa actaaatttt
    10261 ctttcatatc aacaggtatc tcccaataac catcgataca ttgaaataac tcctcgtcat
    10321 actcttcaaa aattggatag attcttaaaa aatcatcaaa tgataagcct tggtcctcat
    10381 agtaatcttt gactaatcca ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa
    10441 taattcctct ctctaccatc gaacgataaa aagtgcattt atctagcgat tgctgggtcg
    10501 tttcaagata atactttaat ccttcttcaa ctaaaggtgg gtaaataatt tccattttga
    10561 ttccctccta aaagttatat attaaactat tttgaagctc ctttaattac tttctttcca
    10621 ttatagtacc cttttaaaga tacatgatgg ctacgtctat aatcaccatt cgcttcgtca
    10681 aatgaaattt ctggttttac tacagtttga tgagttcttc ttaaccgttt cttcgcctta
    10741 gatgtttttc ttgctggtac tgccatgata tttcctcctt ttaccaactt gcttttttta
    10801 ctccaggaat caacccttga tgggcaagtt ctctaaactt aatacgggac ataccgaact
    10861 ttcgcatata ccctcttgga cgaccatccg tctgatcacg aagcctcaga cgattcggat
    10921 ttgaatctct tggcaattta gcgagagcgg tgcgatctcc tgcggctttt aattcctgac
    10981 gtttatctgc atacttttca atcatcgcca tttgtttctt tgctttagca attttcgact
    11041 tttttgccat atacctttat actcctttta ttttgattta gacacctcaa atcgtaatcg
    11101 ttacgttttg acactgatac tttaccaaca agagttgctc ttgtcaacaa taaataacat
    11161 gaaataaaac tcatatgaat agataactat ttgaaaacga atttatcggt acatagagat
    11221 aatcaaatca tttaaaagaa tatgcagttt aggctctgaa aaataagaat tcacttttat
    11281 tagtccaatc aaacacaaat accgttttaa gtagcttttc ctctacttaa aacggcaata
    11341 tgaaagaaac tataactttt tattcaatct tttatgttta actggtttat cgccaatttt
    11401 ttaaaacttt gattattcct tatattataa gaatagacat taagtgaact aattcaatca
    11461 tactgattct ttcgtgtatt caaaattaga tgctgtttaa taaaattatt ctttttgaaa
    11521 aagaattata actatatccc aaaaattgta ggttaattta aagcctattt catcatcgtg
    11581 attgagcttc tttttcatag acgaatttct cagatagttt agttttcttt atgagatttt
    11641 tgtatatttc tgaccttcga tacaactcat tatgagtacc ttgatcaact agttttcctt
    11701 gatccatcac tacaatttta tttacctttt caatagactt caagcgatgc gatataatta
    11761 gtactgtctt atcttgtatt aaacgattta aactttcctg aatacgtttt tcattttccg
    11821 catctaaact ggcagtaatt tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc
    11881 gtgctatgga taggcgttgt cgctctcctc cagaaagtga acttccgttt tctccgacca
    11941 atgtatgata accgtcaggt agtttttcaa taaagtcttc gcagttagct aaacgagctg
    12001 cttttttcac ttcctcatct gacgcttgac tatttccaat acgtatattt tccaaaatac
    12061 ttgtattaaa caaagttacc tcttgaaaaa caatagcgat tttgctaaac aaagaagctg
    12121 gagatacacg tttaatatca tacccatcaa ttaaaataca gccttcatca taatcataca
    12181 gtctagacac taatttcaag atactagttt tcccggaacc actagcacct actaacgcag
    12241 tcacttctcc ttgtttagcc gtaaaagaga tatgatctaa aattggggta ttgttatcat
    12301 atgagaaaga gacatctcgc aactcaatgt caaaagattt cagaggacta tctgatccct
    12361 cttggatact ggtttccttc atagctctaa ttcgctgtat tttaggagct aaataaaata
    12421 tttcaagcac tgcttctttc atagaatcga aggaatcctt tatttttatt gcggctagta
    12481 agtaaccaac gacgtagagt atagtcactt cacccgttaa cagtaagtga acacctacta
    12541 gaatgactac tgctaaacta acatatgaga aaatagagga taaggccatg actgaaaaag
    12601 tacttaattc tacctttaaa tgaatccttt cactctcttc catttttttg aaaagtctgt
    12661 cttgaacttt tttagataga ttaaagctat taatctcttg ctgcaattct atagtttctt
    12721 gaaattcttc cgagttttct ctcaaagtat cgtaatattt agtattggct ttcgtttggg
    12781 atttctttga taacaagatt aagacaaaac taaataacgt tggcaaaata acagctaatc
    12841 ccattttgac attaccaacc aaaagcatca ctgaaataaa agggaaaaac agagccatac
    12901 cgccggcttt aggtattgca tgactcatcg catgctcaat accttcaacg tcagacatga
    12961 tagtttgaga taaatctgat aaattatgtt ttgaaaaata ggataatggt aaatttgaca
    13021 aatcgtctgc aatttgcact cttaaatgcg cactttcttt ataagtacta ttatataagt
    13081 tttcatactc cctatctaac aaccaataca tgaaaagcaa agtaacaaac gatacggcaa
    13141 aataaagcca acggggcttt gtattttcta aaactaattc gtctatcagc atcattataa
    13201 tgaatgctgg taccatatta atgcagtata caaaaaaact agaaatagaa gctttagtta
    13261 aagcttctgc gccctgatca cttaaagcaa atttgttttt ataaaaactt ttcattgtca
    13321 agcctccaat catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta
    13381 gccattaatt cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt
    13441 tcatctacag atttaatgct ggataatcga tgagctatca taataactgt tttttctttc
    13501 atcagatgtt gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg
    13561 gcttcatcca taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt
    13621 tgtttttctc ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg
    13681 ggaaattttt ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt
    13741 gctggcgctt gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc
    13801 gtatcttgaa agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa
    13861 ggcttaccac caattttaat ctgtccctta tccacattgt agaaacctga aattagttta
    13921 gctatcgtac tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct
    13981 aaaactaagt tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca
    14041 aatgcaatgt cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc
    14101 atttctaaat aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata
    14161 cttacataca ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc
    14221 aacaagtcaa cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga
    14281 actatcaaaa tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc
    14341 ccactcaaag aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct
    14401 ttaaatgatg ttactttagc accaaatatc tttataactt gaattccgcg gatatattct
    14461 accgtttcag agcttaacac atctaacgat tcttgatatt tttgaataaa atttgtattc
    14521 tctgtcattt tctttaacaa gaatacacca attactaaca tggcgattag aaccaatcca
    14581 attttaaagt taatgaaaaa gcctaaggca actgataaaa caggtgttac tatggcttga
    14641 gcactatctg gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt
    14701 gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa
    14761 ccgctaattc ccttttttct caaattagtc tctaagcgaa acccaaggat atgagaaaat
    14821 aagccagaaa aaatataaaa taaagctcct aaagttaacc aagcaacagt ttgaaccgca
    14881 taactcttag ctaaatattc atctcctaca ataattagag actgaagaaa tttgtaaata
    14941 ctgtaatatc catagactgt taatacgaca gaaaaatcag aaaataaagt ggctaaccat
    15001 ccgtagtatt tcattttagg gacataacta aataattttt tgtatacttt cattttttcc
    15061 tcccccgcat tcaatttctt ctcttattcg tttacaactg ttaatgcttt ccgtaatagc
    15121 gtaaattaga gatgtacaaa cagataaaag gtaaactacg ttctattttt tgtatttttt
    15181 taagactttt cttccaatca atacaccaat catagataaa cttgctacaa ctaacactga
    15241 aatgattccc catataccta ccgcatagtg gatcattgca tccattgttg cagcagattg
    15301 tcctttatct atccaatctt gacgaaaacg atctgtaaag aaccatatag gaatccactg
    15361 tccggcagca ctgcctaatt gcattagagc ccaagacgca acttgaggaa ttgctcgatc
    15421 atatccaaat acagatagaa taatatcagc taagatcgcc atactaatcg aactgataaa
    15481 ccaaggtaag tacgccccgc ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat
    15541 agtaaaaatt ccttttctag ggtagttata gctaatataa acaaatatga ttccagataa
    15601 taatccaaaa atcccggggg atatgctgtg accaaacgcc ccaccaatac tactaaccat
    15661 tattgaaaaa aagtaaatta ctagggaaat aactgtagc
//
LOCUS       DAJSVP010000055        15111 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSVP010000055 DAJSVP010000000
VERSION     DAJSVP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.057"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON449_001805"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON449_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3934385.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ON449_001806"
     CDS             complement(322..891)
                     /locus_tag="ON449_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3934386.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ON449_001807"
     CDS             1077..1979
                     /locus_tag="ON449_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3934387.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON449_001808"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON449_001808"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3934388.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON449_001809"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON449_001809"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3934389.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON449_001810"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON449_001810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3934390.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ON449_001811"
     CDS             8547..8732
                     /locus_tag="ON449_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3934391.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ON449_001812"
     CDS             complement(8794..9990)
                     /locus_tag="ON449_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3934392.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ON449_001813"
     CDS             complement(10160..11596)
                     /locus_tag="ON449_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3934393.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ON449_001814"
     CDS             11889..12698
                     /locus_tag="ON449_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934394.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="ON449_001815"
     CDS             complement(12884..15037)
                     /locus_tag="ON449_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3934395.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4418 a         3201 c         2670 g         4822 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg g
//
LOCUS       DAJSVP010000056        14627 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSVP010000056 DAJSVP010000000
VERSION     DAJSVP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.058"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(192..758)
                     /locus_tag="ON449_001816"
     CDS             complement(192..758)
                     /locus_tag="ON449_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3934396.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(758..1714)
                     /locus_tag="ON449_001817"
     CDS             complement(758..1714)
                     /locus_tag="ON449_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3934397.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1714..2370)
                     /locus_tag="ON449_001818"
     CDS             complement(1714..2370)
                     /locus_tag="ON449_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3934398.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2740..4758
                     /locus_tag="ON449_001819"
     CDS             2740..4758
                     /locus_tag="ON449_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3934399.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4828..5034
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5185..7599
                     /locus_tag="ON449_001820"
     CDS             5185..7599
                     /locus_tag="ON449_001820"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3934400.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7947..9500
                     /locus_tag="ON449_001821"
     CDS             7947..9500
                     /locus_tag="ON449_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3934401.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9881..11665)
                     /locus_tag="ON449_001822"
     CDS             complement(9881..11665)
                     /locus_tag="ON449_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934402.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11680..13401)
                     /locus_tag="ON449_001823"
     CDS             complement(11680..13401)
                     /locus_tag="ON449_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934403.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13501..14262
                     /locus_tag="ON449_001824"
     CDS             13501..14262
                     /locus_tag="ON449_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3934404.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4747 a         2706 c         2915 g         4259 t
ORIGIN      
        1 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga
       61 tttggttgga cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg
      121 aaatcaacgc tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt
      181 gcgtttttta tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg
      241 ttgataaacc gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa
      301 tcaaccatat ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat
      361 cgtccgcgag gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc
      421 atagtgatga ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg
      481 agattttgtt gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag
      541 acacgttctt ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc
      601 tggatatcaa aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta
      661 tcgtggccat tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc
      721 agtaatgtat ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta
      781 catcatattt tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga
      841 cttcccattt tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg
      901 tcaaacgatg gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga
      961 catagtcagg acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg
     1021 ttttgcgcat cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg
     1081 tgcgtctgac attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat
     1141 gcgtacaaac acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat
     1201 aatcatgtgc tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt
     1261 ctacccataa atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca
     1321 gacaatccgg tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct
     1381 caaatcgttc tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa
     1441 cgatgtattg ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt
     1501 ggattggaag agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa
     1561 aagtacaacc gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga
     1621 tcggcacctt gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat
     1681 atcttttatt tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt
     1741 atctatttgt tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact
     1801 gagtaagtag ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat
     1861 cccgacactt actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt
     1921 ttgaacaaaa gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc
     1981 atgtccacta gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc
     2041 tcgcatgaaa aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga
     2101 aacgaaccaa aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc
     2161 aaataaaatg atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt
     2221 cagatcagga taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca
     2281 cgacagatgg aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc
     2341 agccacctga aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt
     2401 atagcatttt tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc
     2461 tttgaataac caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta
     2521 atgcaaatga aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa
     2581 cgtagacgta ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata
     2641 tgattacaaa aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata
     2701 attattaata atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa
     2761 gaacggagac gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc
     2821 gtgggaactg cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt
     2881 caagcggatg aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca
     2941 gaaattgcta cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg
     3001 atcgcacaag cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat
     3061 tataatcttt ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt
     3121 gaatttttaa ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt
     3181 gctgaatctt tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat
     3241 tactatgccg gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg
     3301 acagggcgtt atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact
     3361 tacggattga cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca
     3421 ggtggcaata atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca
     3481 aactcaggtt cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga
     3541 atctcaaata aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac
     3601 aatttcattt atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg
     3661 acgaataatt ccggtaacaa taccaactct tcaggaaatg ctggtacatc aaacggagga
     3721 caaacaactg gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat
     3781 gatcacggaa ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt
     3841 tatccaggac aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact
     3901 ggctcaaata ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca
     3961 gcttcaggaa cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa
     4021 tacggaatta cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac
     4081 ccaggacaac aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca
     4141 ggttcgtcaa ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa
     4201 ggcacttata ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc
     4261 acgatggatc agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag
     4321 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
     4381 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
     4441 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
     4501 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
     4561 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
     4621 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
     4681 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
     4741 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
     4801 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
     4861 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
     4921 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
     4981 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
     5041 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
     5101 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
     5161 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
     5221 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
     5281 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     5341 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     5401 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     5461 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     5521 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     5581 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     5641 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     5701 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     5761 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     5821 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     5881 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     5941 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     6001 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     6061 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     6121 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     6181 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     6241 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     6301 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     6361 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     6421 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     6481 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     6541 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     6601 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     6661 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     6721 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     6781 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     6841 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     6901 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     6961 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     7021 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     7081 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     7141 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     7201 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     7261 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     7321 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     7381 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     7441 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     7501 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     7561 gcggtaccaa acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga
     7621 ctgatgagtt gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt
     7681 ttgttatttc aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa
     7741 cacgttcaat gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt
     7801 tttgtctatt aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac
     7861 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
     7921 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
     7981 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
     8041 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
     8101 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
     8161 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
     8221 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
     8281 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
     8341 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
     8401 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
     8461 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
     8521 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
     8581 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
     8641 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
     8701 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
     8761 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
     8821 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
     8881 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
     8941 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
     9001 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
     9061 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
     9121 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
     9181 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
     9241 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
     9301 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
     9361 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
     9421 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
     9481 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
     9541 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
     9601 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
     9661 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
     9721 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
     9781 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
     9841 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
     9901 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
     9961 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
    10021 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
    10081 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
    10141 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
    10201 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
    10261 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
    10321 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
    10381 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
    10441 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
    10501 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
    10561 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
    10621 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
    10681 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
    10741 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
    10801 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
    10861 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
    10921 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
    10981 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
    11041 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
    11101 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
    11161 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
    11221 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
    11281 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
    11341 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
    11401 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
    11461 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
    11521 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
    11581 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
    11641 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
    11701 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
    11761 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
    11821 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
    11881 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
    11941 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
    12001 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
    12061 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
    12121 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
    12181 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
    12241 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
    12301 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
    12361 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
    12421 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
    12481 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
    12541 catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
    12601 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
    12661 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
    12721 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
    12781 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
    12841 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
    12901 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
    12961 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
    13021 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
    13081 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
    13141 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
    13201 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
    13261 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
    13321 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
    13381 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
    13441 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
    13501 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
    13561 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
    13621 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
    13681 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
    13741 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
    13801 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
    13861 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
    13921 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
    13981 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
    14041 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
    14101 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
    14161 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
    14221 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
    14281 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
    14341 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
    14401 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
    14461 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
    14521 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
    14581 ataggtgcat aaaagcatta cacttaaaat acattgttgc ttacatt
//
LOCUS       DAJSVP010000057        14607 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSVP010000057 DAJSVP010000000
VERSION     DAJSVP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.059"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            52..864
                     /locus_tag="ON449_001825"
     CDS             52..864
                     /locus_tag="ON449_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3934405.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            861..1661
                     /locus_tag="ON449_001826"
     CDS             861..1661
                     /locus_tag="ON449_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3934406.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1822..3090
                     /gene="rseP"
                     /locus_tag="ON449_001827"
     CDS             1822..3090
                     /gene="rseP"
                     /locus_tag="ON449_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3934407.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3158..4867
                     /locus_tag="ON449_001828"
     CDS             3158..4867
                     /locus_tag="ON449_001828"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3934408.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5079..9431
                     /locus_tag="ON449_001829"
     CDS             5079..9431
                     /locus_tag="ON449_001829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3934409.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9575..10048
                     /gene="rimP"
                     /locus_tag="ON449_001830"
     CDS             9575..10048
                     /gene="rimP"
                     /locus_tag="ON449_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3934410.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10072..11247
                     /gene="nusA"
                     /locus_tag="ON449_001831"
     CDS             10072..11247
                     /gene="nusA"
                     /locus_tag="ON449_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3934411.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11269..11562
                     /locus_tag="ON449_001832"
     CDS             11269..11562
                     /locus_tag="ON449_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3934412.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11559..11870
                     /locus_tag="ON449_001833"
     CDS             11559..11870
                     /locus_tag="ON449_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3934413.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11883..14189
                     /gene="infB"
                     /locus_tag="ON449_001834"
     CDS             11883..14189
                     /gene="infB"
                     /locus_tag="ON449_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3934414.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14216..14563
                     /gene="rbfA"
                     /locus_tag="ON449_001835"
     CDS             14216..14563
                     /gene="rbfA"
                     /locus_tag="ON449_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3934415.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4981 a         2579 c         3259 g         3788 t
ORIGIN      
        1 tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag aatgttacgt
       61 ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt taataccgat
      121 ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg ggcgcaaaac
      181 cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca
      241 aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc gactgaaaac
      301 tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga cttctttgat
      361 acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac
      421 gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat
      481 actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat cgtgactgct
      541 gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga aatcgatgat
      601 gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca agatcctgag
      661 ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg gcagatcgca
      721 tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa
      781 gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa
      841 gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg gcgctagtct
      901 tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca gcaatcttag
      961 cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta agttttgaag
     1021 gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga tggtttttct
     1081 ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg attcttttag
     1141 gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt cctgtgcttg
     1201 tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg
     1261 ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca tatatggttg
     1321 gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa acgattgaag
     1381 gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta ttgctatacc
     1441 cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg attatttttt
     1501 cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat tatgatgtga
     1561 aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc gacagtttgc
     1621 tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac ctgcgtttct
     1681 ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac tattggtgtc
     1741 tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa
     1801 caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt ttttggtata
     1861 ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg aatcctcgtc
     1921 cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa agacgggaca
     1981 acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg caatggggat
     2041 gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc tgacggcatc
     2101 gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc gatggaattg
     2161 agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg agacgaaaca
     2221 gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga cggaacagaa
     2281 atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca gcgtatgctg
     2341 accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt catcattctg
     2401 gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga gatcatgcct
     2461 aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga
     2521 aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc aggtaaaaca
     2581 ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac accgaaatct
     2641 gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat gaatacaggt
     2701 tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt ggaaattttc
     2761 aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg acctgtcatg
     2821 atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat tagtttgatg
     2881 gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc cttagatggc
     2941 gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag tcaagaaaaa
     3001 gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt gctagttaca
     3061 tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt ataataacaa
     3121 acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa aaatgttgat
     3181 tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa tcttgctaag
     3241 agctggatat atccgccaag tatcagcggg tatttattca tatctgccat tagcaaatcg
     3301 tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga
     3361 aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc gttacgaaac
     3421 atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc
     3481 aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat ataagaaatt
     3541 acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg
     3601 tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg caacggaaga
     3661 aagtttggac gagacatata aagattacga aaaagcctat accgaagtat tcaagcgttg
     3721 tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga aagactcaaa
     3781 ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga ctgaaagtga
     3841 ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat cgcatgaaac
     3901 acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg aagtagcaga
     3961 tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg cggatgaaca
     4021 acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac tgaaaaactt
     4081 tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat atctaggtgc
     4141 tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg
     4201 ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat tccactataa
     4261 aaatgtgaat ccaaaacgcg atttcactgt gctttcttat gaagatcttc gttttgtaca
     4321 agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag gaatcgaaat
     4381 cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa ctgtactaga
     4441 cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg taagtcgttt
     4501 attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc caaaaggaat
     4561 tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc aaacaagcct
     4621 gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg ttgatgacag
     4681 aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc caatcagaat
     4741 cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga aaactggcga
     4801 aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct tgaaccaagg
     4861 cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt ttttgacaat
     4921 ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa ttttttaatg
     4981 taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga taagatgcga
     5041 tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa cgagatttat
     5101 ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat ccactgctta
     5161 ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc
     5221 tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag catctgacta
     5281 tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac cagacattcg
     5341 atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa tgcgatacac
     5401 cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct
     5461 tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg ccattagtag
     5521 aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa gtggatgagc
     5581 agcaagcgga acgtgtattg aaactttttg aagaacgcaa acaagaacag gcagaagcct
     5641 ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag gaaagaaaag
     5701 agcaatcgcc agctcttgaa ggacccatcc agctaggacg aaatattcca gccgatgaac
     5761 caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg
     5821 tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca
     5881 cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag
     5941 tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata
     6001 cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc
     6061 gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga
     6121 gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcagga aaatggggac
     6181 accgagctat cgccattaca gaccatggtg gtgcgcaagc ctttccggaa gcacatagtg
     6241 ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg
     6301 gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca tacgtcgtat
     6361 ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag
     6421 tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc
     6481 cattgtcacg aacaacggta gatctgactg gtatcacgga tgaaatggtt cgagggtcaa
     6541 agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag
     6601 cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc
     6661 ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat
     6721 tcaaacgttt cggattaggt gtgttatcta aaaagtttgg tgttagcttg gaacagcatc
     6781 accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg
     6841 caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag
     6901 attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac
     6961 tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac
     7021 gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg
     7081 ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag
     7141 caaaattcta tgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc
     7201 aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg
     7261 gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag
     7321 atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc
     7381 atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt
     7441 taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct
     7501 gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag
     7561 atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg
     7621 agatcattca aaaacgatta gaaaaagagt tgaattctat taatggtaat ggcttctcgg
     7681 ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag
     7741 gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta
     7801 atccattagc ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg
     7861 gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct
     7921 taaacaaaga cggtcatgat attccatttg agacattttt aggattccat ggagacaaag
     7981 tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga
     8041 aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca
     8101 aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg
     8161 cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc
     8221 caggggggat catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat
     8281 atccagcaga cgatcaaaat tcagaatgga aaacgactca ttttgatttc cactcgattc
     8341 acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc
     8401 ttcaagattt atcagggatc gacccacaaa cgatccctac ggatgacccg gaagtgatgc
     8461 ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag
     8521 ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgtta gaagaaacgc
     8581 atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat
     8641 ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg
     8701 gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat
     8761 ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag
     8821 aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt
     8881 caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc
     8941 gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac
     9001 gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa
     9061 tgaaagaaat cacggacaag ggattagatg cttccgtaaa agaaaagaat caactgacag
     9121 tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt
     9181 ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag
     9241 ctgtcccaag tttgggaacc aacgtggcga aacaaatcgt tgaagcaaga aaagacggtc
     9301 catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat
     9361 atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg
     9421 atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt
     9481 tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg
     9541 aattacgcac ttaattttta aggaggcgaa acttttgagt agcgtcgttg aaacagtcac
     9601 agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag tcgaatttgt
     9661 aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg gtatcgatat
     9721 cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag cagatccaga
     9781 tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc ggccattgaa
     9841 aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct tataccaacc
     9901 agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg aacagcttac
     9961 attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa aaaatattgc
    10021 aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa aatgagtaaa
    10081 gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg
    10141 atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa
    10201 aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa
    10261 gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc
    10321 aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttgga
    10381 cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga
    10441 acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa
    10501 cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa
    10561 caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc
    10621 cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta
    10681 ttgcgtcgct tatttgaaca agaagtacca gaagtctatg atggaatcgt tgaaattgtc
    10741 agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat
    10801 atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat
    10861 gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatc
    10921 gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag
    10981 gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa
    11041 aatgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac
    11101 atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa
    11161 gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa
    11221 acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg
    11281 aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc
    11341 cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga
    11401 cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt
    11461 ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat
    11521 gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa
    11581 aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac
    11641 tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa
    11701 gtgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg
    11761 agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa
    11821 taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt
    11881 gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg
    11941 tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta
    12001 gcagtgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg
    12061 cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa
    12121 caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga
    12181 gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca
    12241 gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca
    12301 acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata
    12361 aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat
    12421 tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga
    12481 aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg
    12541 tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca
    12601 tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag
    12661 aagaagtcga tccagataaa ttagtctcac gtccgccagt agtaacgatt atgggacacg
    12721 tagaccacgg gaaaacaacc ttattggata cattgcgcca ttcacgtgta acaagcggcg
    12781 aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa
    12841 tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa
    12901 gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag
    12961 ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa
    13021 ttgataaacc aggtgcaaat cctcagcatg ttatgcaaga attaagtgag tatgaattaa
    13081 ttcctgaagc atggggtgga gaaacgattt ttgttgagat ctcagcgaaa ttcggccaaa
    13141 acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag
    13201 atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc
    13261 ctgtgtcaac cttacttgtc caagaaggaa cacttcacgt tggtgaccca atcgttgtcg
    13321 gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag
    13381 caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc
    13441 gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcaaaac
    13501 gtgcattatt ggaaaaccgt gctgccacaa gccgtgtgac tttggacaac ttatttgaaa
    13561 gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt
    13621 cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa
    13681 tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca
    13741 acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata
    13801 ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa
    13861 ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg
    13921 ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag
    13981 gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag
    14041 gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg
    14101 aatgtggtgc gatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat
    14161 ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc
    14221 taactaccgt gatcgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg
    14281 caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg
    14341 ggacttacag caagcaacga tctattacag cattttatct gacttagcat cagataagaa
    14401 aaaagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa
    14461 tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg
    14521 gaaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa atcccgcaaa
    14581 tcctgtgaaa tcaggggttg cgggatt
//
LOCUS       DAJSVP010000058        14486 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSVP010000058 DAJSVP010000000
VERSION     DAJSVP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.060"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..1296
                     /locus_tag="ON449_001836"
     CDS             127..1296
                     /locus_tag="ON449_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3934416.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1870..2439
                     /locus_tag="ON449_001837"
     CDS             1870..2439
                     /locus_tag="ON449_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3934417.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2426..3286
                     /locus_tag="ON449_001838"
     CDS             2426..3286
                     /locus_tag="ON449_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3934418.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3476..4180
                     /locus_tag="ON449_001839"
     CDS             3476..4180
                     /locus_tag="ON449_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934419.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4185..6020
                     /gene="walK"
                     /locus_tag="ON449_001840"
     CDS             4185..6020
                     /gene="walK"
                     /locus_tag="ON449_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3934420.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6017..7333
                     /locus_tag="ON449_001841"
     CDS             6017..7333
                     /locus_tag="ON449_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934421.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7334..8203
                     /locus_tag="ON449_001842"
     CDS             7334..8203
                     /locus_tag="ON449_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3934422.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8258..9067
                     /locus_tag="ON449_001843"
     CDS             8258..9067
                     /locus_tag="ON449_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3934423.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9170..9799)
                     /locus_tag="ON449_001844"
     CDS             complement(9170..9799)
                     /locus_tag="ON449_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3934424.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9969..11261
                     /locus_tag="ON449_001845"
     CDS             9969..11261
                     /locus_tag="ON449_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934425.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11337..11999)
                     /locus_tag="ON449_001846"
     CDS             complement(11337..11999)
                     /locus_tag="ON449_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3934426.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12224..12508
                     /gene="groES"
                     /locus_tag="ON449_001847"
     CDS             12224..12508
                     /gene="groES"
                     /locus_tag="ON449_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3934427.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12559..14184
                     /gene="groL"
                     /locus_tag="ON449_001848"
     CDS             12559..14184
                     /gene="groL"
                     /locus_tag="ON449_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3934428.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4922 a         2413 c         3053 g         4098 t
ORIGIN      
        1 ggtacaaaat acctaaaaaa gctgtcattg agtatatatt gaaaatacaa gatgaaggta
       61 aaaatcatgc tataataaaa tagtcaaaat ttttgccgat atttcagttt aagaggagag
      121 attgaattga aattaagagc aaacataatt gaaaaaagag gaacattaca agtagttgta
      181 tcttataaag actctaacgg aaaaattaga caaaagtgga aagatacaaa attaaaaaag
      241 aaaggaaata aaaaacgagc agaaaaaatt agagatgaat ttttactaga attggaaagt
      301 caattagcta tggaatgtga atgtgctaat ccaaatttat tgttttatga ctatttgctt
      361 aattatttgg aagtatcaaa aaaacaagta gcatttaaca catatgttgg gtataagcat
      421 tacgtccata atcgaattta caagtttttt tatcctaaaa aaattaaact aaataggtta
      481 aagccttatc atttacaaga gttttatcaa tatatgttaa atgataactg tacagcaaat
      541 acggttattc attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa
      601 ttgataagta caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct
      661 caagtttata atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc
      721 tatttggtag ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta
      781 aaatggtctg ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa
      841 aatccagaga atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat
      901 cgtacattac ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa
      961 caagataatc gtattctttt aggagatgat tatattttag aagataatga gtacgtattt
     1021 actatggaag atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg
     1081 attaagaaaa atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca
     1141 ataatgttag atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg
     1201 tattcgacaa ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca
     1261 gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg
     1321 ggtatcgctt cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa
     1381 tgaaaaaatt cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat
     1441 agagcatttg gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt
     1501 acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga
     1561 aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat
     1621 ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt
     1681 tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga
     1741 aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa
     1801 agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga
     1861 gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca
     1921 ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga
     1981 acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc
     2041 tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga
     2101 tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact
     2161 attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc
     2221 tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa
     2281 ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga
     2341 gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc
     2401 gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc
     2461 aggtacggat gtgccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa
     2521 aattatttat aaagatcagg tatatacaga taaaattgac attacctctg aagagatcta
     2581 tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga
     2641 aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag
     2701 tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga
     2761 agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc
     2821 agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt
     2881 agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg
     2941 acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc
     3001 atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt
     3061 ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc
     3121 tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga
     3181 gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac
     3241 tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa
     3301 acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa
     3361 gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag
     3421 cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa
     3481 aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc
     3541 caaagaaggc tatgatgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc
     3601 tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga
     3661 agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga
     3721 ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc
     3781 attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc
     3841 tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca
     3901 tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt
     3961 cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt
     4021 acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg taactgtacg
     4081 tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg
     4141 tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc
     4201 gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct
     4261 cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat
     4321 ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga
     4381 atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa
     4441 acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag
     4501 gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca
     4561 atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca
     4621 atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa
     4681 gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct
     4741 cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac
     4801 agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga
     4861 aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag
     4921 ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg
     4981 tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga
     5041 tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga
     5101 ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa
     5161 acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag
     5221 tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc
     5281 atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt
     5341 cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag
     5401 gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag
     5461 accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata
     5521 cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt
     5581 ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa
     5641 gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga
     5701 ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc
     5761 ataataacgt ggttttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga
     5821 accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga
     5881 caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg
     5941 cagagagtaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg
     6001 aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat
     6061 ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc
     6121 aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc
     6181 cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa
     6241 cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct
     6301 ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg
     6361 ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt
     6421 agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca
     6481 attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga
     6541 agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa
     6601 atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc
     6661 gatcaaactg aaaaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa
     6721 tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt
     6781 ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg
     6841 agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag
     6901 caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga
     6961 ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg
     7021 tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa
     7081 tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat
     7141 catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg
     7201 ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta
     7261 tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt
     7321 ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg
     7381 gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat
     7441 tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac
     7501 ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct
     7561 tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt
     7621 gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt
     7681 gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat
     7741 tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact
     7801 agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat
     7861 cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca
     7921 tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat
     7981 caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct
     8041 agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt
     8101 agcgatcgaa acaagcgata aagtggtcca aattgagagt gtcaacgctt tcagcaatac
     8161 gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga
     8221 aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt
     8281 taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa
     8341 aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca
     8401 aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca
     8461 tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca
     8521 gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt
     8581 tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca
     8641 tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt
     8701 gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta
     8761 tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt
     8821 aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat
     8881 gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa
     8941 caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg
     9001 cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc
     9061 gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg
     9121 gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga
     9181 ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg
     9241 cgtaatctgc catgctgatc gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac
     9301 ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat
     9361 aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag
     9421 ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg
     9481 tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa
     9541 gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt
     9601 tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat
     9661 ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca
     9721 cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc
     9781 caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg
     9841 ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt
     9901 cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga
     9961 gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga
    10021 atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg
    10081 ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag
    10141 acaaaacagg attttctatg tattttacac aagtatacca ggctacattt gatgtgatag
    10201 ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct
    10261 taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc
    10321 agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca
    10381 tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga
    10441 agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca
    10501 cttttacgct tatcggttca gcggtttttc aacagttgtt cagaatgact gttgttcaag
    10561 gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc
    10621 ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg
    10681 tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcgaatgca gaaatcttag
    10741 cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg
    10801 atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg
    10861 tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt
    10921 tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg
    10981 ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg
    11041 gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg
    11101 gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt
    11161 ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac
    11221 agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt
    11281 cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat
    11341 actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt
    11401 gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct
    11461 aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg
    11521 atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg
    11581 aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct
    11641 tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat
    11701 acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat
    11761 ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt
    11821 tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca
    11881 gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt
    11941 gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg
    12001 taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa
    12061 ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag
    12121 cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa
    12181 gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg
    12241 tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt
    12301 agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg
    12361 tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt
    12421 cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg
    12481 aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat
    12541 caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct
    12601 atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc
    12661 cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg
    12721 atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca
    12781 gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg
    12841 acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc
    12901 attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca
    12961 actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat
    13021 aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca
    13081 atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat
    13141 cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa
    13201 aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg
    13261 ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt
    13321 gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta
    13381 aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca
    13441 ggtggtacag taattactga cgatctaggt ttagaattga aagatgtaac aatcgaaaac
    13501 ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct
    13561 ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca
    13621 acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta
    13681 gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt
    13741 gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt
    13801 acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca
    13861 acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac
    13921 gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt
    13981 ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact
    14041 aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca
    14101 gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca
    14161 tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct tatctacaac tgatcattag
    14221 tgaaagacag ataaaaagag gctggaacag aagcgtttaa ctccaagaaa taagaagaaa
    14281 ttcacggaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt
    14341 ctgcttccgc tgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
    14401 cacgctcttt tttgtgtgta attataatga aaatagttat ttttaatagt tataatattt
    14461 tatctattaa aagtaaatat ccattc
//
LOCUS       DAJSVP010000059        14449 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSVP010000059 DAJSVP010000000
VERSION     DAJSVP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.061"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(324..893)
                     /locus_tag="ON449_001849"
     CDS             complement(324..893)
                     /locus_tag="ON449_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3934429.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ON449_001850"
     CDS             complement(967..2256)
                     /locus_tag="ON449_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934430.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="ON449_001851"
     CDS             2610..3458
                     /locus_tag="ON449_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3934431.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="ON449_001852"
     CDS             3472..4545
                     /locus_tag="ON449_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3934432.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ON449_001853"
     CDS             4535..6706
                     /locus_tag="ON449_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCR3934433.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON449_001854"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON449_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3934434.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="ON449_001855"
     CDS             complement(9117..11303)
                     /locus_tag="ON449_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3934435.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON449_001856"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON449_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3934436.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="ON449_001857"
     CDS             complement(11525..11965)
                     /locus_tag="ON449_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3934437.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="ON449_001858"
     CDS             complement(12039..12512)
                     /locus_tag="ON449_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HCR3934438.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12822..14288)
                     /locus_tag="ON449_001859"
     CDS             complement(12822..14288)
                     /locus_tag="ON449_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3934439.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4118 a         3020 c         2551 g         4760 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
    12661 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
    12721 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
    12781 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
    12841 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
    12901 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
    12961 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
    13021 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
    13081 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
    13141 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
    13201 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
    13261 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
    13321 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
    13381 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
    13441 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
    13501 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
    13561 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
    13621 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
    13681 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
    13741 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
    13801 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
    13861 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
    13921 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
    13981 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
    14041 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
    14101 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
    14161 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
    14221 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
    14281 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
    14341 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat ttaatacaca
    14401 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttat
//
LOCUS       DAJSVP010000060        14259 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSVP010000060 DAJSVP010000000
VERSION     DAJSVP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.062"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..394)
                     /locus_tag="ON449_001860"
     CDS             complement(<1..394)
                     /locus_tag="ON449_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3934440.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEK"
     gene            complement(384..2558)
                     /locus_tag="ON449_001861"
     CDS             complement(384..2558)
                     /locus_tag="ON449_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR3934441.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2574..4916)
                     /locus_tag="ON449_001862"
     CDS             complement(2574..4916)
                     /locus_tag="ON449_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934442.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4938..7481)
                     /locus_tag="ON449_001863"
     CDS             complement(4938..7481)
                     /locus_tag="ON449_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3934443.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7491..7889)
                     /locus_tag="ON449_001864"
     CDS             complement(7491..7889)
                     /locus_tag="ON449_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3934444.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7970..8254)
                     /locus_tag="ON449_001865"
     CDS             complement(7970..8254)
                     /locus_tag="ON449_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934445.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8278..9276)
                     /locus_tag="ON449_001866"
     CDS             complement(8278..9276)
                     /locus_tag="ON449_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3934446.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9269..9616)
                     /locus_tag="ON449_001867"
     CDS             complement(9269..9616)
                     /locus_tag="ON449_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934447.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9632..10282)
                     /locus_tag="ON449_001868"
     CDS             complement(9632..10282)
                     /locus_tag="ON449_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934448.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10293..11465)
                     /locus_tag="ON449_001869"
     CDS             complement(10293..11465)
                     /locus_tag="ON449_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR3934449.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11484..12356)
                     /locus_tag="ON449_001870"
     CDS             complement(11484..12356)
                     /locus_tag="ON449_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3934450.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12378..13298)
                     /locus_tag="ON449_001871"
     CDS             complement(12378..13298)
                     /locus_tag="ON449_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934451.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13298..13567)
                     /locus_tag="ON449_001872"
     CDS             complement(13298..13567)
                     /locus_tag="ON449_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934452.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13564..13944)
                     /locus_tag="ON449_001873"
     CDS             complement(13564..13944)
                     /locus_tag="ON449_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3934453.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         3964 a         3070 c         2265 g         4960 t
ORIGIN      
        1 tcttttcaaa aaagaatgtt tttctctcaa tttttttgac gtttctatca tgaatcacga
       61 gaaaacttgg gacactttta acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc
      121 ttggttcttt tcttgtttca attcgatata cattcctaga gaattttttg tcgtatcttt
      181 ccaagaaaga ttcaacttct tggcattttt tacaatcatt tcttgctaga tacacaaaaa
      241 atgattgatt cgttttttga agttctagta cttcttctaa attactattg attagttttc
      301 catttgattc ttcgttagtt tttatcagtt taaaacaacc aaataagagg aaactaacaa
      361 taagtatcct aacaataact ttcttatttc gcatattgtt tctccaaatt tggtttccga
      421 atatagacga ctcgcggtgg ttcctggacg tcttcagctg ttcgatacac cacaaattcg
      481 cctgttctca atgctcgaat gcgattcgga ttgaatttaa agcggtctac gtctcgtttc
      541 gtgcctcttt ctcctgtcca atcaaccttt cccatatcag aataacctgc ttcttgtgtc
      601 gtcatttccg ttaattcaat gtcttcttct gtccccatta aatctgccca ataatcaatt
      661 tctgtcgggt catttgtctt cccaataaag aacgtattca cattcccaat caactgtttt
      721 cgaaggacag gatcaattct atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc
      781 accccagcgc ctcgtccctt attgactaag tctataaggt tatcatttag ataagaagct
      841 ggttcgtcaa atattactag gactttcgag cgattggctg tttctcctgc ttgtttatac
      901 acttctgacg caaagaaatt aatatctccc acaagcattt gagccaatgt ccgaataaac
      961 tcactataaa tcaatccgtt taacgagata taaacaatct ttccttcttt aacaacttct
     1021 tgaatatcta attctctttt tcctccagaa gtatcaaaga gttcaccaag ctcactatcc
     1081 aataacatat tgatattggt ctgtaaattc tcagaagtcg catacaaata aaacgggttc
     1141 ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg
     1201 agagaaatgt tttcttttaa taatagataa tacgtttcta agtcaacggt tttacgatca
     1261 tgttcaacga ttcgctcttc acttaactgt tccacatcgg caaaatcacg ttctgttttg
     1321 tccatcgctt cattcttttt cgtttttttc ttcgtggttt tcttcttttc tacgaccact
     1381 ttttctttaa ataaatctgg atttttcaac tgaatacgat ccgcaaacaa atgtaagacg
     1441 tttcgtggca ataaataatg ttgtaaccaa actagatttc tttcaatttt atggctcttt
     1501 tggaactcgt ctaaaagctg aatcgttacc tgtaataacg ctttggctgc tgtcgaataa
     1561 tacacgcttt ctgtttctgc tagattggtt aatttatctc gaatggcaat atcattgcca
     1621 ttttttacag gattataacg caaatcgcca ccgtccacaa agacttcaat cgggcgattg
     1681 tatgcctctg ccacttcttt taacgcttgt aaagtctctc ctgctccttt cccatcgact
     1741 aagataacgg ggatacgaaa cttacacgca tgttctgcta gtaattggat caccgtcgtt
     1801 tttcctgaac ctgtagtcgc tggaataatc acatgttggt tcaattcact aaagttcatc
     1861 acataaggga gcccatactc atctgttcct aatagaatca ctttggctaa tgaagccagt
     1921 tgtttattcc gcaaagcaac gtcttttatg acaattgcta cctggtacgc ttgctgatac
     1981 tctgctgtat atttttggga ctcctgaaat ttattcttcc gacgttcctt gtattcttta
     2041 ctatttagca aagctgtttg ccgttctttc tttgaaacaa cttttctttt ttcaataaaa
     2101 gacgcatacg caatcaaaca acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc
     2161 gtttgtccat taaactgcaa cgcatgagaa aacaagttta atggaaataa taagtaacac
     2221 gacaaatacc ctctaggaag aacaaaaata aaacaaaata gatttaatag cgtaaaacct
     2281 aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga agctctcata ctcaaagtca
     2341 taaatatgtg cacgtgtctc ttttttatct atcataaaga caatcgctaa aggaatactg
     2401 cctacaataa aaaatggata tagacaaaca cctaataaag tgacgataaa ataaagttgt
     2461 tgaaaccgac tttttggcgt ttctttttct tgttttcttt tgcgatcgac agaaaacatt
     2521 tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac tatcgatttc ctcctatcgt
     2581 tttcgttgtt tcatttctct tcgataaatc ggttgttttt ctcgttttcg tttcattttt
     2641 gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt gttttggtga ttttgtttgt
     2701 ttcatggttg agtatgcttt agatacggtt gaaggcgctt tggtttgtgc tgttactcga
     2761 ttgactgctg acatatgttc ccgaaccccc tctggacgtc gaacagattt cggtgtggta
     2821 gaacctgttg ctcgtggtac attccctttt cttagtggtt tttgtaccgc atgtttttgt
     2881 cctgcaactg tccgggtttt catcgaactt gtttttgtta cgggagtaag tgcctccttc
     2941 cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta
     3001 tgcttctggc cataagactt gtgtttttgc tttggattcg ctaatgacac caccattgca
     3061 gaaccctgat tgcgatacgg actacgagac gcaccttttc tacttctttc tcttagacta
     3121 ggattgcctt gcaattctcg ttgtaataaa tcggctgttt ttcttgcctg tttctccatt
     3181 gctgttcgtt gcatacgttc ttgactttgc tcttctgctt gtttcgcttg atcatgcaag
     3241 tctcttgctt tttgtgccat acgttccata cgatttggtg ctttatgacg aggttgttgt
     3301 gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg
     3361 tctgagccac ccccacgttt tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct
     3421 ttttgtttct gtgcttttct cgcttctttg cgttctttca accgttgttt ggcttccctt
     3481 ctcaattttg cttctgcccc aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc
     3541 attcttccag taaatagact gcctaaaaag cctcggaaca aataaattcc caacggcgca
     3601 aacaaataaa caatgatcaa gagaattttt tcccatggat cgtcttgtgt actacttaat
     3661 tggaacccgt aactaagaaa agaagaaaaa aggatacatg caaaacctgt cattcctttt
     3721 aaaaacgtca aaataccaaa aactttggca tagttaccaa acacattcat tgtgacaaga
     3781 aacaatccta aaaaaagtaa gaacggcaac atcggtacta acaatagttg catagttcca
     3841 ataatcaatc gaaacatact taagacaaaa taaacaacgg tttgaatcag attcacgaca
     3901 aaataaaaca aggcaaatgc tgtatttttc caactattgt aatattgtat gttcttgttt
     3961 ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa ttttctcccc tatttttgta
     4021 cctaaatcat tgtccaataa aagattgatt cgatcatatt cttttccagc atatttgact
     4081 ttcttctcac gaattgtgtc tacattggtt gtgccataat ttaacaacag atatggttca
     4141 taaacattac tataaaagac ccgagccgcc atcaatttgc ctgccgtttc cactttttta
     4201 tccgctgtta cctcttctgt ttcttttgtt tcataatttg tttcttcttc aggtgttaac
     4261 actggattga ccttcacgaa cgccgtttcc actgtagaat ctaaagaaaa aagaacatca
     4321 aaaagattct tatttgtccc agaatctttt aatacgctta acccagtaaa aataaggatc
     4381 gtcataagaa agacacggag tgcttgtttc attcgagcct ctccagcata tttgattgcc
     4441 atcataattg ccgaaaaact gattccaata gttcctgcaa tacttaacat attgtccgca
     4501 attttttgtg ttaagttttg aatgggttct cgaatggcgg aaataatatc taattcaaag
     4561 agaaattcaa ccattgaagc attgaattct cctaacattt ttacgccact ccaaacaaaa
     4621 tttttcatca ctcctgcaac attgtacgat agcttttttt taaggttcat tgactcatag
     4681 tcattggtca tcaattcaaa cgaattcttt ttgtaatttt tatacaattg atccacatca
     4741 gtcgttactt cttttacttc tactttatcg ttggcattct ctccttcaga acgatcggtt
     4801 tcagtgacac ccgcaccatc attcatttga tcggcagaaa cagtagcccc ttgtagacag
     4861 aaaaaaagac cccctaataa aaggagcgtc caccatttca gagatttttt tcgcaaaaaa
     4921 accacctctt ttttgtttta tcgttgtttt tcattttcta acattgcttg aattttttca
     4981 tcttgatttt cagtagaaga aacagccaat aaccaactat caaacaacac atcaatggca
     5041 atcggttgat ttcttcccaa atgatcctgg aataaacagg ttcctgtttg taattgttcc
     5101 atcatttcta aattggctga attcacttcc atatcaaaga aggacaatag tttctctcgt
     5161 gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct
     5221 tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct
     5281 tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg tatcttcata cccttttgcc
     5341 tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag aaaagatata cgtaaacttc
     5401 ataatgactt ccataattgc cattcctgca agttgttcgc tatttaatcg acccgaatca
     5461 atctcttttt gtgtcggaat gcgtattcct tgtgtcccta acacgttgat ttgtgaatca
     5521 aaacgtatcg gcgtactgtg ctcattccca atgagaatct ttcctaaacc aacgctatgc
     5581 cctaaaatca atttagctag ttctttgtca ctttttcgaa tttcttcgat aacttgactt
     5641 aacgaatgct tacctggcat ggtgtaaact acatcgatgg cgtcttggat caccgttttc
     5701 ttgtggctcg ctgtttctcg attggtatct acttctccta acattcctaa cacattttta
     5761 gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag gatcaagcat ccctcgacag
     5821 ctttcatctg aagataacga aataaaatta attcgttcat acatagccac aaactctggt
     5881 atgtgtccga atttttcaag tgcctttaaa aagaactttt gggtttcatt tttcggatca
     5941 atataaagaa ttttttgacc taaaaacatc agccataaaa aaatatattt tacaaggacc
     6001 gattttcctt tacctggtgg accaataatc aacgtatttc cattggtatg ttccgcgcct
     6061 tcaatacttt tctttgttaa attgggataa taccatacta gtttactaga tagcgctaac
     6121 gcttctttcg cagatttaaa cttttttgca ataacaatcc gtcctaaagg aaatcctttc
     6181 ttatttccta cttctttctt taagtccaag ccataatctg ccacaaaacc tgtcgtagcg
     6241 acctgctcgt aactacgaaa acgttgcgta cttccaatta agcattgatt aaacaacgtt
     6301 agctgatccg ctaccgatcg ataaaatttg aaatctgtag actcaatcgc aaattcaata
     6361 tctttgactc gttcttccaa ttcttctttt gaagaagcag atacaaccag cgtgacggta
     6421 gaataacaca atcgtttttc tttgcgcttc agcttatctt ttacatccat taaccgttct
     6481 gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt caacggtctc atattctttt
     6541 tcttgttcat aaatccgtcc agccatttta ttaatttttc gttcatcaga ttttgaatgc
     6601 tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata agctatcctg aagattttga
     6661 ataaagctac tcccatacat ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa
     6721 tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt cattcgaaat gacgccctcg
     6781 caaatttcca ttggcgtaag ctgtctttca ggcaattttt cttctgatcg atggaaaaaa
     6841 taataataaa tttgtccaat ctcttgggca gataagcgct ctatcggctt ataattggac
     6901 aattccgtat agagctcctt ttctaatgtt tcatacgttg gtaacacact acttatagga
     6961 actcgttgtc ccgtaatacg atagataaac tttttaaaaa catgcttaag caattgaaaa
     7021 tattcgatcg gatcagacac tttcatttct gtagtgaaac gcacaaacag atacgttcga
     7081 tactcgttta attgcacttc gtcttttaaa atctttcctg cctgttcaaa gtacactttc
     7141 ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct
     7201 aggttaaatt gacgtggaat attgataaaa tgatagtcat attcatcatg tccaagtatg
     7261 ccttcgccat cttgaagata gttcttgaaa aaatcaatgt catttaaagg aaatacttgg
     7321 gaagaaagtt gatagcctac ccacacatca tcatttttgg tcaaaacaaa atttcggata
     7381 atggcgtcta tcggaaattc taatcgagga agcttcgttt ttttcttttt gaactgccaa
     7441 atcccgataa taaagacgac caaaagaaac aaaaacgcca tttactcgac tcactccttc
     7501 catttgtgta gggaatacgt cactttttca tggaaaaacg gcacatacat ttcatgttca
     7561 aagcgttgtt tccgtttctg ataataggac gttcgcccaa ttaaatactc gaaaaagtgt
     7621 ttattgtccc atttcattga aaacaatgtc caaacgagaa caccaatccc aaaaatcata
     7681 atcaaccagg actttcgaaa cagtgtctgc aagaaactaa tttgattcac atacgcaatc
     7741 aaaaatagga tcaaaagaat gaagagaaat accccaccta tgactaattg ttccatgcga
     7801 atgccattag aaatccaggg aattttttgt cctttaatcc cgtaaatttt gtgagggtgc
     7861 ttaaactcct ttgtatagtt gtattccatg ttcactctac ctccttttac aaaaaaagaa
     7921 gcgaccagaa gttctaatct tctgatcgct tttgcagttt gtctttttct tatgataatt
     7981 gtatccaggg cacaatcatt tgcgttaccc ccacgttctc ttcactatcc ttaataaaga
     8041 taatgttctt tagccattca ccaatatcaa cggcattcga taacaataag acgagcatta
     8101 tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag
     8161 caccaaccat tagaacaatc gcaacgattg ccaatagcca aatgacttgt ccagatacat
     8221 catcaaataa cgatttaaga tcaaaaagtt tcatttccat tctcctctaa gttcatttta
     8281 ttcatttaac gtagtaacaa aataactatc gccacttgtt tttacttcca ttgaaaagtt
     8341 tgattggaac tcgtttcctt tctcaaaata gaaacgatac ttcccttgca aaaagtagtt
     8401 tccttcttta ttttttactg catgatccag ttgtacattg atcaattcgc cgccttctaa
     8461 ccctttttcg ttagaaatca atgccaattt ttcatcattt ttgacatata actcaaaaaa
     8521 ccgtttcaca aatgcttcca gcttgatttc ttcgctttca gtgaccgctt gcccagaaga
     8581 ataaaatcga ttttcctgat agttcacaga attggtattc ttactctcag aaagattcaa
     8641 aaattgaacg ccatcaagta cttttaaaac attgttttga taactgactg gaaaagaaag
     8701 ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc tcataaaata ggcgatacat
     8761 tgcttgtgtc aaatcgattt cttctagttt aataaagcga acatctttcg ctactcgatt
     8821 ggtagccgtc gttacaaagc ctagatcaga cgcttccaat ccttgtgcta aataatgatt
     8881 gacggaagcc tcccattcgt ttcgtttttc tggcgaataa gtaaataacc tttcaataaa
     8941 atgttgtccc tcgtacttca attgttccgt agaagacaag gtttggttga cttcagttaa
     9001 caatgtttct ggattgactt gttttttcac tgctaaacgc gttaatgtat ccactcgtcc
     9061 aaagtttagg aggagcacga cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa
     9121 tacactcatg gcactttttt gcgaaaattg gatcattcga tctgacggtt ctgttttctt
     9181 tttttggaac aaaaaacgtt tcttttgttt cttttccttc tgttgtttcg gtggcttagt
     9241 ttggaacgct tgtggattaa attttgcatt actcatgaga aatgccatac cgttctaaca
     9301 ataatttcgg tattttgtcc acaatcacta cttcgtggac aaccacactt gccccctcca
     9361 aaaaggcaag tgcttctttg taggcatttt gactgtattt ttttgataaa gcatgacaaa
     9421 tttcttgata aaatagttga ctgcctaccg aataccgatt attgtgtatc cgccacaaaa
     9481 cgaacacact gatttgaatc gacgggcgta aaaaagaaaa attctttcca taagaaaaag
     9541 aacgtccttc ttgggtttct tttgcttgtt ctgttggtac ttcttgtacc tctcttattg
     9601 attcttgtct ctccataacc ttctcctttt cttattcttc taaaagctgt tgattaaagc
     9661 tacttcccaa attttgcata aacgtgattt tctttgacgc tggatcaaag gttacgtcaa
     9721 aaatattcat ggcattattc caggaactat caccaatttt attgcgatac gacaccacaa
     9781 cgagcccttg gatagtcgct ttttgtgtgc tttttaaata aacagaacat ttctctaatt
     9841 cagaagacac cccgtttgaa tcaccatagg aaacgccttc tttgaaatat tgcttcatca
     9901 gtttgtcaga aagatatggt gcacaattgt tttttcgttc ttgaaaagaa tcaggctcat
     9961 aattaaacag aacctcgaac gcttctgtca ctgttttttg aaaggtttga atgtttcctt
    10021 tttctgttga atcattgttt ttcaattctt catttgcttt ttttaacgtc gtgacctcgc
    10081 tttctacttg ttgaagttgt tctttgagtg acgttacaag gtcttcctgt cctttcttct
    10141 cttcttggaa cttgtttatc tccaccgtca gtttctcttc ctttgctgtt agctgtagct
    10201 tttcatgaat aagaaaaaat gtactgccta caccaaataa gaacaatccg cctacacaaa
    10261 cataaataaa tcgtttcttc atttgacacc tcttatttct ttatcggtcg agcaaaacaa
    10321 atatcaccac cattaagcaa atacccaaag gataaattga cttgcccttc acctacaccg
    10381 ttagtattca ttcccccttg ttcgattact ttcgtagaaa gtccatgcca atcttccgct
    10441 aaaacagctg tgtgtccatc atttcccaca ccagaaccta agttcacaat gataatatcg
    10501 ccagcttttg cctcgctctc aggtatttct tgtaagtatt gcttacttcc tcttgcgtcg
    10561 ctcgtcattg agccggtaaa ccacccaacg ttcgcaggcg ttttataccc tgctttcgtt
    10621 aaaacaagcc acacaaagct cgaacaatcg gcatagccat ttttatctgg attttcgaca
    10681 ctaccaaatt gtgcccgttg cggttgtgaa taatgaaaat aaccaatcag actttttgct
    10741 acgtttaata cattactatc ggaagaactg cttgttcctg ttgtttctag acaataatcg
    10801 gtcaagtctt tacttcctgc gtttgcccca ttttctaatg taattgaagc gacgggatca
    10861 ctgtcattct ttcctagttt tgcgtaccat tgattcgcaa aagtaatacg attccccatc
    10921 attggtatcc ctgcacgctc aaatttcact tgccactgtt ctgttgcttc ctctacgctt
    10981 tttgcttgta atgcaacttg tgaagctttt tctgttgtgt ttagttcatg aatcaaaaac
    11041 tcaacttgta cacctaaatc tgaccactct ttgtttcgct ctttggcaaa atttttcaaa
    11101 gcagtcgttc gccctgccgt ccattgtgca atcccatgcc cagataatgg atcgtttggt
    11161 cgttcaatcg ctgtgggact catactcgac tcttgttgaa aattgcctaa catacccgct
    11221 agaccctcta atgtcgtatt tggaatatgt ttttgtactt cctttgcaat ttgaagcccg
    11281 tttgtctcca tgccttggtt cgttaatgag atagtacttg cgacaatttc ggttggacta
    11341 cagtcgattt cttcctcttc agaaccacta ctaatcaaaa gtatcataaa cagaaaacca
    11401 aacatacata gtaatagtgt gggtagacaa ccgaacccac accataaaag aagttttttc
    11461 ttcatgtttg atccttcatt cgcttagttc attttttgtg ttgcttttct tttgcgccaa
    11521 aaagcaaaac cgcttcctga agccaataat ccaccaagtg ctgtcgcaac gagagacact
    11581 tcttcgccag tcttaggcaa tactttcatt tttccatcag accctttcac cgcaatattt
    11641 ccgcttggaa gttgttcgac cttgtctgaa atgggcgtca agccatttcc tgattcttta
    11701 taagcgacac catcaacgac ggagacaatt ttttctcctg aagctgtttc aattggtgta
    11761 cttggtgttg gaatatgggt taattcagac gcattattgg tttcaatttg aacattttgt
    11821 ccacctgttt gttggctgcc aatacttcca tctggtttta ctacgacgtc ctgtactgga
    11881 ggcgtagtgt tcgtcccccc attattggta ggtggtgtcg ttggaggagt cgtatttcct
    11941 tcattgcctc ctggattcga cggcttttct ggtgtctctg tattaccacc atttcctggg
    12001 ttttcctcaa cgggtggaac ttcaggtgtt ccttcccctc cgccagtatc tggattttct
    12061 ggtacttcag gcgtttctgt gcctccacct gtatctggat tttcttccgt tggtggaatc
    12121 tctggggttt caggagtttc tggtatttcg ggaatttctg gggtttcttc tcctccatct
    12181 gttggtggta aaacaggttc ttcaggaact tcaggtgttt cttccgttgg tggaataatt
    12241 tctgtcggtg gtacacttgg atcacctggt gacgtagtat ctgcatacgc actggttcct
    12301 accataccta gcaatacagt tgcactaaat aaacttccta aaatcatttt tttcatgtgt
    12361 ttttcctctt ctcttattta ttgatttatt tcaaaatcat ctagttcggg aagcgtggaa
    12421 actagataat ttttttcctg ttcttccaat tgaaaagaca ctttttttgt ttcttgtgtc
    12481 tttttctcgg tttccaatgt aatgacatac gcaatttgcc aatgcttgcc ttctttcttc
    12541 atttcccagg ataaaatact tttgactttt tccttttgag gactgatttg tttcgcaact
    12601 ttttccgtta gaaacgattg aagctttttc ctataccgtt cttctgccat ttcacccgaa
    12661 taataattcg taataaaata acgactaaat gtatcaattt gtggttgttt tgtcgcaagt
    12721 tcttccgcaa atgcaatgcg ttcttgtaac tggtgctcct ttttcgttag ttgttcaatc
    12781 tctctttgcg ttgaaagttg cataataaaa agaccacaag atacaacgac gataaatcca
    12841 aacaaaagcc attgaaaaga catttttttc ttctgaagag agccgttgct ttgtgtaaac
    12901 gctgtttcta cagaaagtgg tgttttcttt tcctttttga tttttgttgg tcgacgttcg
    12961 ttcttttggt ctttctttac ttgataaaac ttcggtaatt cggaatttaa ggtttgaata
    13021 aaggtttgtg cttcttcact taattgccca ttttctgtta aagattgttc aatcaatgaa
    13081 atcaaatgaa actcttctac tggaaaatca tacgagcctt caaatactgg aatttcatgg
    13141 ttttctgtgt agatttccaa ggtacaccag ccaatttccg ctcgtgccaa tagctcttca
    13201 ttttctcttt tcaattgaat aaatgcctta tctaatgcaa tttttttatc tgttagtaaa
    13261 gacatgttat caacttctgt aaatcgaatc atcatcatta gtcaaacact tcttccacca
    13321 cttctaccgc atacaccact tgaatagggt tatttaaaga aaatttcacc cgatattctt
    13381 gattgttcaa actgacgata tagctatcct catcttcagc ataataggta tattcgttgc
    13441 ctttgacttg aaaatatttt tggatcgctt gtctttgttg ccattcgcca atctttggaa
    13501 aataggcgaa atgatagcca aataaaacgc ctatccctaa taaaacaatt cctgttacta
    13561 taatcatctg gtttcctcct gtacctgttt atcttctatt gactcttttg tcggtaagca
    13621 ataaagaacc agtaacacaa gagagccaat cgttgggaca aaatagaaaa aaataaatgc
    13681 ccaatgaaaa cctgcgtctc gtaaacgtcg aacgctgaga gaaagagacg ggacaagaac
    13741 tacgaaacaa tacacaatca atacgcctat caaacaacca attattgttt cagaagcatt
    13801 caacgctcct gctagtccaa tcgctaagcc taggccaaac ccaatgaggg cattcattaa
    13861 cgcaacgagc caatactctt ttcttgtggc tttccctttg taattaaagc tattctgcca
    13921 aaatttttta taatttgtga tcatatcttt gtcctttcta atgtttattt ttgattctag
    13981 tttcttctgt tttactaaca atgaaacttg ccataaaaaa ggtggccaat acaccaccag
    14041 ctagaaatcc acttacaaat aacaaaattt tttcctcctt tctaaaaaat ttcgccataa
    14101 aaaggcaaag tatgaacacg ataaatacca tgtacatact ttgccttttt atgcaatata
    14161 tgttaactct ccaagcaatt actgattttt aaattcttta taaataaaat aaaaatcgtt
    14221 tgagtaaaga aataagtcaa tgaagaccca aacgataaa
//
LOCUS       DAJSVP010000061        14148 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSVP010000061 DAJSVP010000000
VERSION     DAJSVP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.063"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(264..2261)
                     /gene="tkt"
                     /locus_tag="ON449_001874"
     CDS             complement(264..2261)
                     /gene="tkt"
                     /locus_tag="ON449_001874"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3934454.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2362..2613)
                     /locus_tag="ON449_001875"
     CDS             complement(2362..2613)
                     /locus_tag="ON449_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3934455.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2759..3382
                     /gene="lexA"
                     /locus_tag="ON449_001876"
     CDS             2759..3382
                     /gene="lexA"
                     /locus_tag="ON449_001876"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3934456.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3562..3780
                     /locus_tag="ON449_001877"
     CDS             3562..3780
                     /locus_tag="ON449_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934457.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4069..4581)
                     /locus_tag="ON449_001878"
     CDS             complement(4069..4581)
                     /locus_tag="ON449_001878"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3934458.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4595..6892)
                     /gene="recJ"
                     /locus_tag="ON449_001879"
     CDS             complement(4595..6892)
                     /gene="recJ"
                     /locus_tag="ON449_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3934459.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7034..7477)
                     /locus_tag="ON449_001880"
     CDS             complement(7034..7477)
                     /locus_tag="ON449_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3934460.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7478..8266)
                     /locus_tag="ON449_001881"
     CDS             complement(7478..8266)
                     /locus_tag="ON449_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3934461.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8263..9204)
                     /locus_tag="ON449_001882"
     CDS             complement(8263..9204)
                     /locus_tag="ON449_001882"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3934462.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9381..9968)
                     /locus_tag="ON449_001883"
     CDS             complement(9381..9968)
                     /locus_tag="ON449_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3934463.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10189..11496)
                     /gene="obgE"
                     /locus_tag="ON449_001884"
     CDS             complement(10189..11496)
                     /gene="obgE"
                     /locus_tag="ON449_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3934464.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11790..12800
                     /gene="gap"
                     /locus_tag="ON449_001885"
     CDS             11790..12800
                     /gene="gap"
                     /locus_tag="ON449_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3934465.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12885..13820)
                     /locus_tag="ON449_001886"
     CDS             complement(12885..13820)
                     /locus_tag="ON449_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3934466.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14006..>14148
                     /locus_tag="ON449_001887"
     CDS             14006..>14148
                     /locus_tag="ON449_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934467.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQG"
BASE COUNT         3945 a         3153 c         2487 g         4563 t
ORIGIN      
        1 accatatccc tagaaaatca ctaaattacc aaacaccatt agaagttttt ttgagttacg
       61 taaatggaaa gttttgtctc gcttaatttg acaaataata ttctcaagaa acacaaacta
      121 atccgaagaa ttctttgttt tgtcatttaa atgtaacata gccgaaaacg aaaagtaata
      181 ttgatttatt cttctaacac ataaagaaca gctgtctcca acttgcagac agctgttctt
      241 atcagtcaaa caattgcttg gatctatgat agttgattgt acgtattcac gacattttct
      301 actgtaaatc caaattcttc taaaatttta gtgccagggg cagatgcacc gaaatgatcg
      361 attgtaatcg ttgcgccctc tgttcctacg tagcgttccc atccaaatga tgcagcagct
      421 tcgatagcca cacgtttctt cactgattta ggaagtacac tttctttata ttcagaagat
      481 tgtttttcaa agaggtcaaa gcttggcata gaaacaacag aaacatcttt gcctttttca
      541 gccaattctt tttgagcttt cacagccaaa tcaacttcag aacctgttgc aatcaaaata
      601 ccttctggtg tttctccttg tgatggagaa agaacatacg cgcctttttt caccatttca
      661 tctgctactt cttttgtaga aggcaatact ggaaggtttt gacgactcaa tacaaggata
      721 gtaggcgcat ctgttgtctc catcgctact ttccaagcag cacgtgtttc atttccgtct
      781 gccggacgaa tcacttgaac accaggcatg cagcgaacag atgctagttg ttcaatcggt
      841 tcgtgcgttg gtccgtcttc tccgacagca accgagtcat gtgttaagac gtaaacaaca
      901 ggagtatgtt ggattgctgc taaacgaact gctggtctta gataatcgac aaagacaaag
      961 aatgttccac cgtagatacg agttccgcca tgaagttgga taccattcat agccgaagcc
     1021 attgcaaatt cgcggacacc gaaccagatg ttgcgtcctt cataatgttc aggagtgaaa
     1081 tctttatctg cagccaccat tgtattattg gaacctgaca agtccgctga accaccccag
     1141 aagcttggta tagctttcga taattcttgg atgacttctt tacttgaaac acggcttgct
     1201 tgactgcttc caacttcata agttggcaat tctgcatccc agttttctgg tagtttgcca
     1261 tcaaaagcat cttcaaattg ttgtgctaat tcaggataag cttttttata attggcaaac
     1321 atttcattcc atgcatcttc agctttttgt ccttcttcta tcatcgtttg atggaagcgg
     1381 gcagctactt cttctggaac agtgaaatct ggatactccc aaccataaac ttctttagcc
     1441 atcttgatgc catcttcacc aagaggagca ccatgaacag cagatgttcc ttctttagga
     1501 gcaccatagc cgatgactgt ttttacttcg atcaatgttg gtttgtctgt ttcagctttt
     1561 gcttcttcaa tcgctttaga gattgcttct aggtcatttc cgtctttcac taagatatgt
     1621 tgccagccat atgcttcata gcgagcacca acgttttctg tgaaagcttt tgaagttgga
     1681 ccgtctagtg aaatatcgtt tgaatcgtat aaaacgatca atttccctag tttcatatga
     1741 cctgccatcg aacttgcttc ttgagatacc ccttccatca agtctccgtc accgcatatt
     1801 gcatacgtat agtgatccat cacattaaag ttttctttgt tatacgttgc tgcaaggtgc
     1861 gcttctgcca ttgccatacc gacagccatt gcaatccctt gtcctaatgg ccctgtagtg
     1921 gcttctacac catctgtatg atgtacttca ggatgtcctg gtgttttaga atcccattga
     1981 cggaattgtt tcaaatcatc gattgtcact tgatagccag ctaaatgaag caagctatac
     2041 aacattgctg acccatgtcc tgccgaaaga acgaaacggt cgcggtcagc ccagtttttt
     2101 gatgtcgttg gatttacttt caggtgtttt gtccataacg catatgccat aggtgctgcg
     2161 cccatcggca atccaggatg tcctgagttt gctttttgga ctgcttcaat acttaatgta
     2221 cgaattgtgt taacgccaag ttggtcaatt ttgtcaaaca aaataacccc tcctattaat
     2281 ttaggttttt ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc
     2341 aatctttttc agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt
     2401 ttggatttct tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat
     2461 tccttcgata tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt
     2521 gatttgttct tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc
     2581 attgatccgc tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca
     2641 tattatataa aaactgaaaa aaaacaacta gataaactaa ataaaatacg aacattcgtt
     2701 tgatttagcg ctttttatat ggtactattt atttatcaag tgaaagaagg tgtaccttgt
     2761 ggcccgacaa actgaatcaa gacaaatcga agtcttacga tatatccatg aacaagtaac
     2821 ccaaaaagga tacccgccga ctgtccgtga aatcggggaa gctgttcagc tttcttctac
     2881 ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc aaagagatcc
     2941 gacgaaacct cgtgcaatcg agttgacaaa acaaggttta gaaaagattg ggattcgtcc
     3001 aacaactatt ccagtacttg gcgtcgtaac agctggagaa cccattttag cagtagagga
     3061 tgcttcagac ttctttccaa tccctcctaa tctttcttcc gaagaaggca gtctctttat
     3121 gctgacaatt cgtggagata gtatgatcaa tgctgggata ttggatggtg acaacgtgat
     3181 cgttcggaag caagaaactg ctcaaaatgg cgatattgtc attgcaatga ctgccgagga
     3241 cgaagcgact tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc agcctgaaaa
     3301 cgacttgctc gaaccaatca tcttagatca agtatctata ttaggacgag tagtaggact
     3361 ttatcgtagt catatctatt aattgctact aattaaaaga aaacttttta aaaagagttt
     3421 gagacgttgg tctcaaactc ttttttcgcc cgggttcttc ttctttcaaa tttttatcac
     3481 ttccctcttt acaaaccgaa caaatattcg tatactaaat atacgaacaa ttgttcgcat
     3541 aaattaaaga ggtgagaatt catgaaatca atgagacgtc ttggcttagt attaactgtt
     3601 ttgtttttag gtattattat tggtaatttc ggcatacgtc cagctttact tgtgtttgtt
     3661 ccgttattta ctttgtggtt catgttgtgg gatgaaaaga agtaccgtca atctgagcgg
     3721 cgcagaattc atgaagatca tttctatcat gagccctact acagatccag gcaatcataa
     3781 taaggattga ctgctaatta ttcaaaagac aagaagttaa acttttacgg gggtgtgaca
     3841 aaaatcggtc acatcctttt tttcgaataa acggtgttct aaagccagtt tttcggtatt
     3901 aagtcaaatt gcggaaaaag tgatttttta aacttctact ggtctttgct tgtttccatt
     3961 tataaaaaaa tagcgaccct caaaagtcag atagagaatc tgacttttga gggtcgcttt
     4021 aaaatcgctt ctttaagaat aacatcaaga atagcttctt ttttctgctt aatattccat
     4081 taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca tggagatcca tcaattcgat
     4141 caaaaatgcg cagcctacga ctacaccgcc taattgttcg atcagttcga tcgtcgcttt
     4201 gattgttcct ccagtagcta gcaaatcgtc acaaatcagc acacgttgac ctggtttaat
     4261 ggcatcctta tgcaatgtca atgtatctgt tccatattct aatccataag tcacttcgat
     4321 ggtttctcga ggtaacttcc cttttttgcg gactggggca aaaccgactc ctaactcata
     4381 agcaacagga catcctacga tgaatccacg ggcttctggc ccaactacca tatcaattcg
     4441 tttttctttt gcataattta cgatttgttt agtagcttcc cgataagcgt caccatctgc
     4501 catcagtggg gaaatatctc gaaacacaat tccttttgac ggatagtcgg gaatactggc
     4561 aatataatct tttaaatcca ctctttgttt cctcctaggt tgttaaccat ttctttatgg
     4621 taggaatatc gctcaaaagc aaaaattcct caactttgat tttattgagc cgtcgctggt
     4681 ataatctgct ctcagtcaaa ggatgacttt ctgggctagc gactttttgt aaaacaccgt
     4741 tctctattgt aacaaatttc aattcaaaaa acacctgtat cataaaaatt aataattttt
     4801 cagggatacg caaatattga gctattgctt ttattttatg acgtatatca attcgttctt
     4861 gagaatggat caatttaaac aattttgcgt actgttctct tgtgcctacc ccattcaaat
     4921 aggcctcatc aggtgaaatc cccatcagat aaacacgatt gaatgaataa gaccgaaaaa
     4981 cttctttcag atccgctaaa tcatctggac aatccataac aactagagag tttggttttc
     5041 ttttttcaat tattgccttt acagaatcta gtccttcgaa aacaatcgca gcttcctgga
     5101 taatctcagg cacgaatttc aacgtcgaag aatcaaatgc taaataaagg ctaccttccg
     5161 acgttcggct ctgttcacgg aaacgttttg cccgccaatc aaatacttgc agaccaccca
     5221 cagaaaaatc agataccatc agctgcggtt tcttacgccc gttccattca ttgatcgaaa
     5281 gctttcctac cacatctacc tgattattca gcagttcttc ttcttgagta ccaaaaccaa
     5341 aagcgaccgc atcaagctga ctcgatgcat cggaaaggac aaatttcaga tgctgttggt
     5401 ttgctccgat tcttttgata ttttctgctt ggacttgtcg aaataaaaac tgaggcaaag
     5461 gattatccgt accaaatggc gccaatagtt tcagctcatc aattcgctct actgttactt
     5521 catttggcag caatacttca tcaattctca actcaggccc tctcatcaag tcaatttgat
     5581 gatcgactat atattggttc atcttctcct gtagtatagt gacattttca cttggcatcg
     5641 ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa taaatctcgc atctggtcaa
     5701 gcatttcaaa aagattcaag gcttcaatac tacgtccaga tcctttagct gatccatctt
     5761 cttttaaagt aaggacaagc gttggtttac ccgtttcatt cattattttc ccagcaacaa
     5821 tgcccaaaac gccttcatgc catcctcggt ttaccaatag atggatatga ttctccttat
     5881 tgaccatcgc taatgcttct tgtgttatct gctctacgat tgcttttctt tcttcattga
     5941 tctcattcaa tttttttgct tgaacggatg cttcctcttc atcaaacgtc gcaagtaacg
     6001 agacggcagg acgtggatcg cccatccgac cgattgcatt tagtctggga gcaatcgaaa
     6061 aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc gctttcttca aatagcgcct
     6121 gaagtcctat ccgttctgaa tgatgaatgg catcgattcc taatgaaaca aggattcgat
     6181 tctcatcagt caatgaaact aaatcggcga tcgttcctat cgctactaaa tccaaaaatt
     6241 ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa tgctactccg actcctgcca
     6301 aatctggaaa tggatagtgc cccgctggat gacgagggtg aacaatcgca taagcgtctg
     6361 gtaaattctc aggtagttcg tgatgatctg taatgatcac gtctacacca gcttgctgag
     6421 cgtatgctac cgcatcattt cctgctaccc cgttatcaac agtgatgatc aactggattc
     6481 cttcttcgat cttttcttgg tataccgatt ggtttggtcc atacccatgc tcaaatcgat
     6541 ttggcaggaa tacttctact tctgcaccaa gtaattccag tgtttctttc attaccgttg
     6601 cactggtgat cccatctgcg tcataatcac cgtaaacgag gattttttct ccactgatta
     6661 cagcttcttg gattcggctg attgctttat ccatatcgtg tatcagatac ggatcatgta
     6721 attcttgttc ctttggatgt aaaaatcggt tgatatcctt ttcctcatgg taacctcttt
     6781 gccacagtaa cttaccgaca aatggagata atttcagttc acggatcatt tggagaaagg
     6841 attcagaagg ctctgtctcg cttaacttcc aatcaaaatt tgcttgtctc actttctcac
     6901 tcctaactta gttattatag caaaggaaat cagcaaaaaa aagcagaaag aaagaataaa
     6961 aacagatcag atccgctaga cagatgttca gcaggacctg atcttttgta cgaccattta
     7021 tgaaatatcc ctattagtca aaataattga agctttgttt gggcgattga gttttttgtt
     7081 cttccggcgc tttcaattga ctgatttgtg cggttttcgc ttgtaactcc gcctgataag
     7141 cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg ctgttcttct tttgcttctt
     7201 caattttctt ttgaagttct gtttgatatt ctgacatttc ctgagtcagc tgtttgattt
     7261 gctttttttg ctgccatagt gttgccgaag cagtcaaaaa tacaatcagt gcaccaatca
     7321 gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa ggaagtgaag ccaaaactta
     7381 caggaacatt tttattgttc aatacagcaa aaagaacaat aatcaaaact aaaacaaatc
     7441 caagaatcac attgctttgc tttttcaatt ccttcaccta ctttttgtta aaaattccgc
     7501 tggctaaata atcaccaatc gttggaaaca gcgtataaaa ttttgatgca acttctaaaa
     7561 taaacggttg attgatttcc cgttttggat gtctcatatt gcggacgatc atttttgcca
     7621 acttggcagg ttctaaaacg atttgtccga ctttttccaa atagcttccg cttggatcag
     7681 ccttatcgaa gaaatttgtc tcgattggac ctggattcac tgtcgtaaca gctacgccaa
     7741 gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag gactgcaaat ttcgtggcag
     7801 aataaaccgt tgattttgca gttgccattt ttccagccat agaggcgata ttaatgatat
     7861 gaccttgtct tctttctgcc atatcgatcg caaatttttg tgtcaaaacc atcattccta
     7921 agatgttgac ttcaaacata tcgtatgctt ttccaaggtc gaatgtaagg aagtcttcga
     7981 aaatcccaaa acccgcattg tttaccagaa tatcgacgcg cccgacttct tccatcacct
     8041 tttcatataa ttcgtccacg ctttcaggat tgctgacgtc taccttgaaa gcataagcct
     8101 ctttgccgct caagcgtctg cactcgtctc tgacaccttc tacaaatgcc attcttcgtg
     8161 cgcaacttat aacgattgct ccttgctttg ctgcttcata acagatttgt gcgcccagac
     8221 cgcctgaact acctgttact aaaacaactt tatcttctaa attcatgcct cttcctcctc
     8281 gcgaaatgga atctcaatgc tatcaaaatc tttcataatc gtcgtgtttt caaacacttc
     8341 ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa tatctcgcac tgatatgagt
     8401 aagtagcagc cgttttgcct gcgcttcttt tgcaacttca gcagcttgat gagtcgtgga
     8461 atggaaatag ttgtgcgcca ttcttgcttc atctttatta aatgtgcttt catgtaccag
     8521 aatatcgcta ttttccgcaa gcacaacgga attatgtgtt tttctcgtat cgcctaaaat
     8581 agtcacgatt cgtccttttt tatctggacc tacgaaatct tttccattga tcgtccgccc
     8641 atcttctagt tggatcgttt caccttgctt cagtttgccg tacagtggtc cagctggaat
     8701 acctaattct tttagacgat ccacctgcaa ttctccttta tgatcatgtt caaccacacg
     8761 ataaccaaaa ctgtcgatcc catgatttag tttttttgta taaacagaaa attgctgatc
     8821 agtaaaaatc ggggctgttt catttagctc aatgaatttc agcggataac ttaaacgcgt
     8881 ttgcgaaata cctaaagaga cttttatgta ttcctctatc ccttttggcc cgtaaatctc
     8941 taatggcgta tccccacctt gaaatgaccg gctgctgatg agcccgggaa gtccaaaaat
     9001 atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt tttctcggac gaatagtcgt
     9061 tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac aaccaaactt cattgcgctc
     9121 gtctaaaagt ttcaatgcta tacttgtcac atttctatgt tttgcaggaa cacctgcacc
     9181 tgttcctaaa aattgtaatt ccataatcta cctgactttc tttaagttcc tctttaattt
     9241 tagggtaaaa agaagggata atcaaccgta agtcgagaat gggttcaaat taaaaataac
     9301 gttctaaagc gatagcgttt tctttgaaaa ttactataaa aacagcagcg aacccgtttg
     9361 agatcctctg ctgtttttta ttaatttgaa gatccagaac tttcattgtc gttggaagtt
     9421 tgcggatgtt tgtccttcca agtcttttgt gcagcttctt tcgcatgatt ttcagcttct
     9481 ttcgcatcat gcacatcttt ttcgacatct tcataatcca agcgagtaat ttctccgcct
     9541 aattccatca ttaccaattg attcagtggg cgattgtcct cttcatcagc aatcaagatc
     9601 agtcctgtag ctccttcacc gattttattt cctacaaagt cgaacacggt ctgggcatct
     9661 tttatttctt tggcatcttg tgtcgcaccg atcattcctc cggcaaacca tcctaataaa
     9721 atccctagcg gaccacctaa tacaccaacc aacattccga ttaatccacc cgtagaagat
     9781 ttgttattac cggtaaagtc gataaaatct tctattttga attgatgtgt cccattagga
     9841 tcatggctga caactgccat ctgttctcct ttaatcgaac gaacagcatg taactttttg
     9901 atttctgaaa atgcctgata tgcctggctt tctacttgaa aatttaaaat gattactcgt
     9961 ttgctcatta aaaacgcccc tttcatacct tcttgttatt tattttacct ttaattgaaa
    10021 aataagaaaa gaaaagcact ctttttttcc aataaaatag ggctgtgaca aaagtcgtat
    10081 cacagctcct ttttccgaat aaacggcgtt cttgaagctg atcacttctg tcacaacctc
    10141 ccttaaatct gaatctatat tttttggtat ttgtttttat ttgcttgcct attcgacaaa
    10201 ttcaaattca aactcaccga tacgtacaag gtcgccatct ttcgcaccgc gtgcccgaag
    10261 agcttcatcc acacccatac cacgtaattg acgagcaaaa cgcatcactg tttcatcatg
    10321 atcgaaattc gtcatttgga ataatttttc gatcttctca ccagataaga tccacgttgc
    10381 atctgaatca cgatcgattt ggaactcagg accttctgga ttgaatccat agtgtacagt
    10441 ttcttcttcc agttcttctt cgtacaatgg gaattctggc gttacttcta acaaatctgc
    10501 tgtagcattc aataacgcgt ccaatccttg tcgagtcact ccagaaattg gaaatactgg
    10561 gatgtcatca gcaaattcat cttctttttc cttgttcagc tgttctttga atttcactag
    10621 attttccggt gcatccggca tatccatttt gtttgccacg atgatttgcg gacgctctag
    10681 caagcgtaag ttataagtag ataattcttt gttgatggca agatagtctt cataaggatc
    10741 tcgtccttcc atcccactca tatcgatcac atgtaaaatt actcgtgttc tttcgatatg
    10801 acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct ccttcaatca agcctggcaa
    10861 gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc attcctagat taggtacgag
    10921 cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat gaaataacgg atagtaaagt
    10981 cgatttcccg acagatggaa aacctacaag tccaacgtct gccaatactt tcaattctag
    11041 ttcgattttt cgctcttgtc ctggttcacc gttttcagca atttccggtg caggattccg
    11101 aggagaggca aaacggatat tccctcgtcc accgcgtcca ccttttgcca caactagtgt
    11161 ttgaccattt tcaattaaat ctcctaaaag agcaccagtc tccgcatctc ggacagtggt
    11221 tccttgtggt actttaacat aggtatcttc tgaaccacga ccgtgcatcc ctttactcat
    11281 cccattttca cctggctgcg ctttgaaatg gcgattgaac cgaaaatcca tcaatgtacg
    11341 taacccttca tcgacgatca agatgacatc gccgccacga ccaccgtctc cgccagccgg
    11401 tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc attccgtctc cgcccttgcc
    11461 agctttcaca tcgatcgtta cttgatctaa aaacatggac ataattaagt cctcctatta
    11521 ttcatttatt tatttgactg cctgaatttc cacaataaac aaaagtctta cttattttat
    11581 cggtgaaatc tgattttgtc tatcatttca gcaaaaagat tgtttacctt cttctttttt
    11641 tatcatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt ttgacaaaat
    11701 attctttgcg tagcagaata acagtgtatt aattgtatta attgaggatg ccgtttcaca
    11761 gcacccctaa taaaggagga acttttgata tgacagtaaa agtaggtatt aacggatttg
    11821 ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt ctcagatgat attgaagtag
    11881 tagcaatcaa cgacttaaca agtccaacta tgttagcaca attattgcaa tttgactcta
    11941 ctcatggtac ttatcctggc acagttactg caacagaaga ctcaatcgta gtggatggcg
    12001 aagcaactcg tgtttatgca gaacccgacg caagcaaaat cccttgggca aaagaaaacg
    12061 gtgtagatat cgtactagaa tgtactggtt tttatacatc tgaagaaaaa gcaaaagcgc
    12121 atttagatgc tggtgtcaaa cgagtagtta tttctgcccc agctggtgct atgaaaacca
    12181 tcgtctataa cgtcaatgat gatacattag atgaaaacga caaaatcatc tcagcaggtt
    12241 catgtacaac aaactgttta gcacctatgg cttatttctt aaataatgaa tttgggatcg
    12301 aagttggtac aatgacaaca gttcatgcct acacttccac acaaatgctc ttagatggac
    12361 ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc tgacaatact atcccacatt
    12421 ctactggtgc tgcgaaagca atcggcttag ttatccctga gctacaagga aaattacaag
    12481 gacacgcaca acgtgttcca gtcgttgatg gttcattaac tgaattggtt tcagtattga
    12541 aaacaaaagt aactgctgat caagtaaacg aagcaatcaa aaaacacaca gttgacaatc
    12601 cttcattcgg gtatgatgac cgtcaaatcg tttctagcga tgtcattgga acaactcaag
    12661 gttcgatctt tgacccaacg caaacagaag taacaactgc tggtgatttc caacttgtta
    12721 aaactgttgc atggtatgac aatgagtatg gatttacttg ccaaatgatt cgcttactag
    12781 aaaaattcgc taacttgtaa tttgatagat caatcgataa aagaagcctt tgactggatc
    12841 cagtcaaagg cttctttctt ttcatcccct acttttaaac tcgttcaatg aacatattgg
    12901 cttaaaaatt gttggactac ttgctgatat ttttcaggat ctgtcttgta tgcttccgca
    12961 tgctcggctc cttttacaac atacttttct tttggtccct tcgttgcacg atagacctta
    13021 tctaacattt caaatggtac aaatgtatct tcgtctccat gtataaagag cattgggcgc
    13081 tggtttttct tcaattgttc cactgcattt gcttcaccga aaaagtagcc agcacggact
    13141 tttgtcacta gactcgttac ttgcataagc ggaaatgctg gaagactaaa catatcgtct
    13201 agctgataag caagctcatc atgaacggaa gaataaccgc aatcttcaat gattgctttc
    13261 acattatctg gtagtttttc tccgctagtc atcataactg ttgctgctcc catgctgaca
    13321 ccgtatagaa cgatttcttg cgattttcca ttgttttcta gcactttgtt tatccactgt
    13381 acatagtctt tacgctctgg ccatccaaat ccaatgtaat ccccttcgct ttttccatgt
    13441 cctcttgcat caggaacaag gacattgtaa cccatatcat gaaacatctt tgcgtaattg
    13501 gtcatcgttt cagcgtttcc catatagcca tgagccacaa gagctgtttt ttcacttttc
    13561 gtttcagcag gaagataaat cgctgataat ttcagaccgt cttttgaagt gatggtccaa
    13621 tcgcttcggt tttcttttga actaaaccat ttatgtgcat ccgtttcttt ggatgtctgt
    13681 gttgtaccag gtgtatcttc gtctaaaaag tctttctcag aaggcacaac agcataatta
    13741 taaaaataat tccctgcaaa cactaacgca acaatcagag ctaaagcgac gcctaaaagg
    13801 attttcatcc aaagtttcat aaatcatcca tctcatttct cttttttcat ataaccttga
    13861 attagtataa tcaaagaatc tctactatgc aatgttcatt tttataaccc gcgaattgta
    13921 aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa
    13981 agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac
    14041 aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg
    14101 acgtgctata caaactattt ataatgtagt caataagttc aagcaagg
//
LOCUS       DAJSVP010000062        14077 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSVP010000062 DAJSVP010000000
VERSION     DAJSVP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.064"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(110..565)
                     /locus_tag="ON449_001888"
     CDS             complement(110..565)
                     /locus_tag="ON449_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934468.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ON449_001889"
     CDS             complement(719..1927)
                     /locus_tag="ON449_001889"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3934469.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ON449_001890"
     CDS             complement(2039..2371)
                     /locus_tag="ON449_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3934470.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ON449_001891"
     CDS             2519..3394
                     /locus_tag="ON449_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3934471.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ON449_001892"
     CDS             complement(3476..4057)
                     /locus_tag="ON449_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3934472.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ON449_001893"
     CDS             complement(4063..5322)
                     /locus_tag="ON449_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3934473.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ON449_001894"
     CDS             complement(5324..6370)
                     /locus_tag="ON449_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934474.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ON449_001895"
     CDS             complement(6605..6880)
                     /locus_tag="ON449_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3934475.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ON449_001896"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ON449_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3934476.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ON449_001897"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ON449_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3934477.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="ON449_001898"
     CDS             complement(9979..10659)
                     /locus_tag="ON449_001898"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3934478.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ON449_001899"
     CDS             complement(10708..11343)
                     /locus_tag="ON449_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3934479.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ON449_001900"
     CDS             complement(11409..12812)
                     /locus_tag="ON449_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3934480.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ON449_001901"
     CDS             complement(12799..13830)
                     /locus_tag="ON449_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934481.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..>14077
                     /locus_tag="ON449_001902"
     CDS             13861..>14077
                     /locus_tag="ON449_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3934482.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENK"
BASE COUNT         3909 a         2973 c         2416 g         4779 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaa
//
LOCUS       DAJSVP010000063        13898 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSVP010000063 DAJSVP010000000
VERSION     DAJSVP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.065"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..1127
                     /locus_tag="ON449_001903"
     CDS             177..1127
                     /locus_tag="ON449_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3934483.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1645..2100
                     /locus_tag="ON449_001904"
     CDS             1645..2100
                     /locus_tag="ON449_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3934484.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2441..3043
                     /locus_tag="ON449_001905"
     CDS             2441..3043
                     /locus_tag="ON449_001905"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934485.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3137..3469
                     /locus_tag="ON449_001906"
     CDS             3137..3469
                     /locus_tag="ON449_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HCR3934486.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3620..4599)
                     /locus_tag="ON449_001907"
                     /pseudo
     CDS             complement(3620..4599)
                     /locus_tag="ON449_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4604..5371)
                     /locus_tag="ON449_001908"
     CDS             complement(4604..5371)
                     /locus_tag="ON449_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3934487.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5861..6286
                     /gene="lacA"
                     /locus_tag="ON449_001909"
     CDS             5861..6286
                     /gene="lacA"
                     /locus_tag="ON449_001909"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCR3934488.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6303..6818
                     /gene="lacB"
                     /locus_tag="ON449_001910"
     CDS             6303..6818
                     /gene="lacB"
                     /locus_tag="ON449_001910"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCR3934489.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6829..7761
                     /gene="lacC"
                     /locus_tag="ON449_001911"
     CDS             6829..7761
                     /gene="lacC"
                     /locus_tag="ON449_001911"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCR3934490.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7764..8744
                     /gene="lacD"
                     /locus_tag="ON449_001912"
     CDS             7764..8744
                     /gene="lacD"
                     /locus_tag="ON449_001912"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3934491.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8772..9089
                     /locus_tag="ON449_001913"
     CDS             8772..9089
                     /locus_tag="ON449_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3934492.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9089..10795
                     /locus_tag="ON449_001914"
     CDS             9089..10795
                     /locus_tag="ON449_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3934493.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10937..12343
                     /gene="lacG"
                     /locus_tag="ON449_001915"
     CDS             10937..12343
                     /gene="lacG"
                     /locus_tag="ON449_001915"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3934494.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12366..12638
                     /locus_tag="ON449_001916"
     CDS             12366..12638
                     /locus_tag="ON449_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HCR3934495.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12659..13558
                     /locus_tag="ON449_001917"
     CDS             12659..13558
                     /locus_tag="ON449_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3934496.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4512 a         2324 c         2696 g         4366 t
ORIGIN      
        1 ttacaaatta gataatacat tagaagctgg agacaagatt gtctagataa tgtgtgagat
       61 aaaatttttg gcaggttatc atacataaga caaaatgttt ttaaaactga taacgtattt
      121 attccacaac aattacgcat ttatttctga gaaaaatata aagcaggagg attacaatgc
      181 tgaaaactta ttccttaaca aaagatacat tattagtttc gtcagaatca aatatcaaaa
      241 attcttctgt tgtttatgtt tatggtgacg aacgagaata tatcgaatct tctgaacata
      301 aatatgactt taaaataggc aatatactaa cgtttgatga tcgagttgca tatgacacga
      361 tgatagataa gaacgatgaa aaaagtttgc tcgtaagttt tttgtttccc gaggtacatg
      421 aaggtgggca tctagatacc ctgacgacat ctgttgtttt cttggtattt aaaaataagt
      481 tgataatttt tacaaaacaa aaattaaaat tcattcccga actcttatcc aatatggttt
      541 tacgtgatgt caatcatttc gtggaggaag tacttttaaa gatcatgcaa aaagatttct
      601 atatgatgct taacgacctt cgtggagtca aggaaaaaat tgatgattta gactcagaaa
      661 tatcaacgtc cggttcactc aaaccaacgt ttggtgattt attgactctt caaaaataca
      721 tgatagcttt gtcaacaact tataaagcta accataaagc gcttgatttc attaaaaaaa
      781 atttcacaac tattgacgat attgatttta gtactaaaaa aataattgat gatttatacg
      841 atacaagtga tacaatggat agaatcattt taagttacag ccaatatttg gacaaattgg
      901 aaaatatgat aaacaatatg atttcgtatc agctaaacat gattatgaaa accctaaccg
      961 agatatcaat tgttttgact attccagcaa ttatttttgg cttttgggga atcaatgtcc
     1021 atgtcccgtt tgaaaagtct tcttatggtg ttttagccgt attgattatc tctgctatac
     1081 taagtctaat ttgctggctt ttgatgcgac gcaaaacata tttgtaaagt atttgtgata
     1141 ccctaatatg atttaaagca gaatttcttt gttgactttt taaaaggctg attcctttca
     1201 ataatttaat agcttcagcg gctgctcaga agtcaataaa ttggactatt tttattcagt
     1261 tacttaggca acatttgaga tacgattgcg attataaaaa ttgatatacc aaccaatggc
     1321 tgcttggaag actgacatat tagcctcgat atcatcgaat aactaaatac agaaaaatac
     1381 aataaatatt atcaaactag ggactatact agaaaacatc agtacattcc ttaactcagg
     1441 gtctataatt agaaaatcgc ccctttgaaa actgattaaa acagccccca ttatctagtg
     1501 actagataat gggggctgtt tttttgttaa gtgatataat aaaccataaa agaagtttag
     1561 aaatacggtc ctggtcatca ataaataaac aatttaatta attatttatt taattattta
     1621 atttcagaaa ggtatacatg aatcatggtg caacaaattg tcctacccat caaagactcc
     1681 agcgtcttaa agatggtaca agatacactt ctcgatagtt tccgtgctgg tcgccgaaat
     1741 tacaccgttt ttcaagttgg taaggccacg ctactacgtg tgagtgatgt catgacatta
     1801 aaaaaatcag atgtctataa tccagacggc tctgtcaaaa atgcaacctt tattcatgat
     1861 aaaaagaccg gtaaagcaaa cacgttatat ttaaagcctg ttcagcaaga cttgttgcaa
     1921 tatcatgatt ggctggccca agaaaatatc aattctgagt ggttatttcc ctcgacagcc
     1981 cattatgatc ttcatatcac tgagaagcag tttcataagg tgatggcgca tgttggtgat
     2041 ctcttaggaa tcaacttgga agacaaagag aaaaaattgc gagtcagaaa accgttttga
     2101 gatatgaaca gcatttgcct tcacgcctta gcgattttaa ataatagcct tgaaagtgat
     2161 tatggaaaag ccctctacgc tcaatgcaaa attgacgtag aaactatttt tgatcgattg
     2221 aaaggtgtat ttggtatgtg aagaacccat gtcagaggca agaacgttgc acctactgac
     2281 attggaatga tgcttatgag catgaatcta accaaattat cccttgaggc tagaagaaaa
     2341 gcggaagctt ttcaacacaa agcagccaaa aataaaaaac gcgacgaagc aatcacattt
     2401 atgattattc atcgcgtttt ttatatttag agctagttat ttgctagacc cactatttac
     2461 agatgaaacg tttatcacct ttggttttgg gcatttagtg gaattagcag aaccaggtca
     2521 ttatgacgaa aagtggcaaa attggaaact tgaatctttg ccgatttttc ctgatcgata
     2581 cgattttgaa gttgcaaaag ataagggaaa gcagtttaaa attgttgcag aacttctcaa
     2641 aaaggcaaat acaattattg ttgcaacaga tagcgacaga gaaggtgaaa atatcgcctg
     2701 gtcgattatc cataatgcaa atgccttttc aaaagataaa acatttaaaa gactatggat
     2761 caatagctta gaaaaagatg tgatccgaag cggttttcaa aatttgcaac caggaatgaa
     2821 ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt tgatcggcat
     2881 gaacgcaagc cctttgtata cgttaaattt acaacagaag ggcgtacaag gtacattttc
     2941 actaggacgt gttcaaacgc ccaccttata ccttattttt cagcgccagg aagccataga
     3001 gaattttaaa aaagaacctt ttttcgaggt ggaagctaag taaaacaagg gtcatttaag
     3061 ggcgttctaa gccccacaca gcgttttaaa acccaagagg agctttttag cttttgtttc
     3121 ttctaaacaa gctaaaatag gcaatcaaga ggggataatt gctgatgttc aaaccaaaga
     3181 gaagaaaacg aatagtccga gttcgttttc tttaagtagt ttgcaatcaa aagtcaatca
     3241 gctttataaa gcgacagcga gccaaacttt aaaagctatg caaggactgt atgaagcaaa
     3301 attattgagt tatccaagaa cagatacacc atttattaca gagaacgaat ttgcttattt
     3361 aaaagcgaat tttggcaaat atagcggttt tttaggactt gatcttgaaa tgttgttatc
     3421 attgataatg atacgttcaa acaacagcac ccaaattttg acgaattagt gaaactctat
     3481 gaaaaagacg tagtaaaaca tgcaacatcc tattctaatc agctggtaaa gcttaattag
     3541 gttatataag cttgaaaata aagggtttgt ccaatatgga caagcctttt tcttatgttt
     3601 tgccacagat ttgccacact tattgtgtaa gagtatcaag gataggtata atttgatact
     3661 ttgttttttt agtgatgtgc aaataaattt ctgttacacg actacctcta gaatgcccta
     3721 cacggtcttg aattgcttct aggggtattc cttactctgc taagaaacta atatgcgtat
     3781 gacggaatat atgggtcgta atatgtttat taaaactaag tttattatca atatcaattc
     3841 ctgctcgttt tggattacct tttcgattta attttccatt ttcaggtgag ttgaggaaat
     3901 tgttaaggac actataatga agaggtgttc cattatagtc gggtttatga cgacttttgg
     3961 aagtaaaaat ccatccatta tctttataat caggattgaa tctcatatgt tggtgattta
     4021 actcaatttc ctctttaaga atttctatag cccttttaga taattggaca tttcggtaac
     4081 tttgtaagtt ttttacatta ccttcatagc tattttctgc agctcctccg tgagtggact
     4141 gactaatgat gatattttgc ttttcaaaat attgataggc accaatattt tggagtttcg
     4201 actctgtaag tcaacagggc cattagttag aatacctatt gggatgcggt ttttatgtag
     4261 agccagcagt agttgatgta tctcaggaat catcgtaatc tgatcgagtt catattcata
     4321 gtcagcttgg aagtctagag cttctgtcct agaaatacta tcgatgccaa attcttccat
     4381 agtcttctga cagcgaaaaa ttctaagctg ctcaatactg atgagttttt tcgtgtattt
     4441 aggaaaagca acatcagacc aatatctgaa acgtttgtaa atgttatcta tttgttggat
     4501 atcaatcgtt ggaaaacatt tctcaacgct acgtttaaag gggagttgat ggtcataaag
     4561 agtgtcatca aggtcaaaaa caatattttt ataagtcata attctagtcc gcttttaaaa
     4621 tagtagtgta ctggctaaat tcctctaagt gttgagggct aatctggtta tctgtaatga
     4681 tggtatcgag ttgatctaga ttatagaagt taaagaaatc gtatcgatcg aatttagtac
     4741 tgtctactaa taagaatttt tctacagcat tgtttagggc aagttgttga atcacacctt
     4801 ccttgtcact atatgtagca atagaattat gggtaacagc atttgcacta acaaaagctt
     4861 tggcaaatct aattgctttt aaatttgtcg aagccattga acctacaaaa gctccagtta
     4921 tttctctata ttcaccgcca agaagcaata aatcaatggt ttcgctatca tttagaatca
     4981 agaacacagg gagactattt gtaatgacac gaattttata acctttacga cctttcaatt
     5041 ctaatgccag ttgttcaagt gtagttccag gtccaataaa aatagtatcg ccatctttga
     5101 ttaaagagca ggctttttta gcaatgtcaa ttttttcttt tgtatttaga cttttcttct
     5161 cgatatgtgt cttttcaagt ggctttttag aggaaagcaa ttgtgcacca ccatgagtac
     5221 gtgttaaaag tccatcagct tctagagcat caaggtctct acgggctgtc atatcggaaa
     5281 tatctagttc atctattatt tcttttatgg ttatggtccc atcaatcttt aacatatcta
     5341 aaattttttc taatcgtcgt tttttgttca tatgaagacc cttctttcat aaagtaattt
     5401 ttttccaaag ataattctct tttaattgta tcataaaaga caatattttc aaggtaaaac
     5461 aaacaatttc aaacaaaaac aaatgttaga tgatgaaata agaacagagg attgacgtat
     5521 attagcttag gtcagatttt gtataagacg aaaataaagt aggacctctt aatcagtaag
     5581 ttatagaaag taaaagactt ttgtaatact tgaatagata tttcacgtcc attttgtgat
     5641 ggattaaatg aacaaaaatg aacaataatt taacggtgtt atctattttt taaaaaaaca
     5701 aataaaaaaa acaaaaaata aacaaaaaaa gtgttgcttt ttgtttgaat gcttgatatt
     5761 atataaacaa agaaatgatg aaaacgtttt ttcgaacatt ttgcaaaatg ttttctactt
     5821 ctacgtagca tttctttcta aaatttagga ggtggtcaaa atggctattg ttgttggtgc
     5881 agatctcaaa ggtacgagat taaaagatgt tgtgaaaaat tttcttgtag aagaaggttt
     5941 tgaggtaatt gatgtgacta aggacggaca agattttgtt gatgttacct tggcagttgc
     6001 ctctgaagta aataaagatg agcaaaactt aggtattgta attgatgctt atggagctgg
     6061 tccattcatg gtagcaacta aaattaaagg tatggttgca gcagaagttt cagatgaacg
     6121 ctcagcttat atgacacgtg gacataacaa ttcacgaatg attacggttg gcgctgaaat
     6181 cgtcggtgat gagcttgcta aaaacatcgc taaaggtttc gtcaatggta aatatgatgg
     6241 cggacgtcat caaatccgag tagatatgct taacaaaatg tgttaagaaa aggagttaag
     6301 gaatgagaat cgcaattgga tgtgaccaca ttgtcacaga tgaaaaaatg gcagtatcag
     6361 aattcttgaa atcaaaagga catgaggtcc ttgatttcgg gacatatgac cacgcacgta
     6421 ctcactaccc tatctatggt aaaaaagtcg gtgaagcagt agtaagtggt caagcagact
     6481 tgggagtatg tatctgtggt acaggtgtcg gcattaacaa tgcagtcaat aaagttccag
     6541 gtgttcgctc agcgttggtt cgtgatatga catcagctat atatgcaaaa gaagagttaa
     6601 atgcgaatgt tatcggtttc ggtggaaaga ttactggtgg tcttcttatg aatgacatca
     6661 ttgaagcttt cattgaagct gagtacaaac caacagaaga aaataaaaaa ttgattgcaa
     6721 aaatcgaaca cgttgaaaca cataatgcac atcaagcaga tgaagatttc tttacagaat
     6781 tccttgaaag atgggaccgt ggtgactacc acgattaagg tataacaaat gattctgaca
     6841 gtcacactaa atccttcagt agacatctct tatcctctag aaacattgaa aattgataca
     6901 gtgaataggg tgaaagatgt tagtaagaca gctggtggaa aaggcttaaa tgttactaga
     6961 gtcttgtatg aatctggtga taaagttact gctacaggtt tcttaggtgg taaaattggt
     7021 gaatttattg aaagcgaatt agaacaatct cctgttagtc cagcttttta taaaatctca
     7081 ggaaacacta gaaactgtat tgctatctta cacgagggta accaaacaga aattttggaa
     7141 caaggaccaa caatttctca tgaagaagca gaaggctttc ttgaccacta tagcaatctt
     7201 attaaacaat cagaagttgt gacaatttca ggaagtttac cttcaggact tcccaacgat
     7261 tattatgaaa aacttatcca acttgcttcg gatgaaggag tagcagtcgt tttggattgt
     7321 tcaggtgcac ctcttgaaac agttctaaaa tcgtcggcta aacctactgc catcaaacct
     7381 aataatgagg aactttcaca gttgcttgga aaagaagtaa caaaagatat tgaagaactc
     7441 aaagacgttc ttaaagagtc actttttagt ggtatcgaat ggatagtggt ctcattaggt
     7501 cgaaacggtg cctttgcaaa acatggtgat gttttctata aggtagatat tcctgatatc
     7561 ccagttgtta atccagtcgg atcaggggac tcaacggtag ctggtattgc atcagcttta
     7621 aatagtaaaa aaagtgacgc tgacttatta aaacatgcga tgacattggg tatgttaaat
     7681 gctcaagaaa caatgacagg gcatgtgaat atgactaatt acgaaacact aaactcacaa
     7741 attggagtaa aagaggtata aaaatggtac ttacagaaca gaaacgcaaa tctttggaaa
     7801 aaatttcaga taaaaacggt gttatctcag ctttggcatt tgaccaacgt ggtgctttga
     7861 aacgtttgat ggcacagtat caagatacag aaccaactgt agctcaaatg gaagaactaa
     7921 aggttttggt tgctgatgaa cttacaaaat acgcttcttc aatgttgctt gaccctgaat
     7981 atggacttcc agcaacaaaa gcattggata aagaagcagg tcttcttctt gcctatgaaa
     8041 aaacaggtta tgatacatca agtacaaaac gtttgcctga ctgcttggat gtttggtctg
     8101 caaaacgtat caaagagcaa ggcgctgatg ccgttaagtt cttgctttac tatgatgtag
     8161 acagctcaga tgaacttaac caacaaaaac aagcttatat tgaacgtgtt ggttctgaat
     8221 gtgtcgctga agatattcct ttcttcttgg aaatccttgc ttatgatgaa gaaatctcag
     8281 atgcaggttc tgttgaatat gcaaaagtga aacctcgcaa agtcattgaa gcgatgaaag
     8341 ttttctctga tccacgcttt aacattgatg ttcttaaagt agaagtacca gttaacgtta
     8401 aatatgtcga aggttttgct gatggtgaag tagtttacag taaagctgaa gctgctgact
     8461 tctttaaagc acaagaagaa gcgactaacc ttccatacat ctacctaagt gcaggtgtat
     8521 ctgctaaatt gttccaagaa acattgcaat ttgctcacga ctcaggtgcc aagtttaatg
     8581 gtgtgctttg tggacgtgct acttgggcag gatctgtaga accttacatc aaagaaggtg
     8641 aaaaagcagc acgtgaatgg ttgcgtacta ctggattcga aaatattgat gaacttaaca
     8701 aagttcttgt taaaacagct agtccatgga ctgataaagt atagtaaaaa cataaaacgg
     8761 aggatattgt tatgaacaga gaagagatga ctctcttagg gtttgaaatt gttgcttatg
     8821 ctggagatgc tcgctctaaa cttttagaag cgcttaaagc ggctgaaaat ggtgatttcg
     8881 ctaaggcaga tagtcttgta gtagaagcag gaagctgtat tgcagaggct cacaattctc
     8941 agacagctat gttggctcga gaagcttctg gggaggaact tccatacagc gttaccatga
     9001 tgcatggtca ggaccactta atgactactg tcttattaaa agatgtgatt catcacctca
     9061 tcgaacttta taaaagagga gcaaaataat gcataaactc attgaactta ttgagaaagg
     9121 gaaaccgttc tttgagaaaa tctctcgaaa catctatctt cgtgctattc gtgatggatt
     9181 tatcgctggt atgccagtca tccttttctc atctatcttt atccttattg cctatgtccc
     9241 aaatgcttgg ggattccact ggtcaaaaga cattgagacc ttcttgatga ctccttatag
     9301 ctattcgatg ggtatcctag cattctttgt tggtggtact accgctaaag ctttgacgga
     9361 ctcaatgaac cgtgacctac ctgcgaccaa tcagattaac ttcttatcta cgatgctagc
     9421 gtctatggta gggttcctct tgatggcagc tgagcctgca aaagaaggag gcttcttgac
     9481 agccttcacg ggaacaaaag gtcttttgac agcatttatt gcagcctttg tgacagttaa
     9541 tgtttacaag gtctgtgtga aaaataatgt tactattcgt atgcctgaag aggttccacc
     9601 aaatatctct caagtattta aagacttgat tccattcact gtagcggttg ttctccttta
     9661 tggatttgaa ctcattgtta agggaactct tggtgtaacc gttgcagaat caatcggtac
     9721 ccttcttgct cctctcttct cagctgcaga tggttacctt ggaattacac ttatctttgg
     9781 tgcttatgcc ttcttctggt ttgttggtat ccacggtcct tcaattgtcg aaccagcaat
     9841 cgctgctatc acttatgcca atatcgatgc caacttgcat cttatccaag ctggacaaca
     9901 tgcagacaaa gttatcactt ctggtactca aatgtttatt gttaccatgg gtggaacagg
     9961 agctacattg attgttccat tcttgttcat gtggatttgt aaatcagaac gtaaccgtgc
    10021 catcggacgt gcctcagttg ttccaacatt ctttggggtt aatgagccaa tcttatttgg
    10081 tgcaccaatc gtattgaatc cgattttctt tgtaccgttc attttcgctc cgattgtcaa
    10141 cgtttggatc tttaaattct ttgttgacac attgaacatg aactcattct ctgccaacct
    10201 tccatgggtt actcctggtc cgttgggaat tgtactcggt actaacttcc aagttctatc
    10261 atttatctta gccggactct tggtagttgt tgatactatc atttattacc catttgttaa
    10321 ggtatacgat gaacaaattc ttgaagaaga acgttctggt aaaacaaacg atgctcttaa
    10381 agaaaaagta gctgcaaact tcaatactgc taaagcagat gccgttcttg gaaaagcagg
    10441 cgttgctaaa gaagatgtag cagcaaacaa taatatcaca aaagaaacta atgtccttgt
    10501 tctttgtgca ggtggaggta caagtggctt gcttgccaat gccttgaaca aagcagcagc
    10561 agaatacaat gttccagtca aagcggcagc gggtagttac ggtgctcacc gtgaaatgtt
    10621 gccagagttt gacttggtta tcttggctcc acaggtcgct tcaaacttcg atgatatgaa
    10681 ggctgaaacg gataaattgg gcattaaact tgcaaaaact gaaggagctc aatatatcaa
    10741 attgacacgt gatggacaag gtgctcttgc atttgttcaa caacagtttg attaataaat
    10801 ttgtaaaagt tatttgagac gagattattc tttcaattcc ttataatatt gttaaccata
    10861 tcattgcatt tcatttcctt ggtttccgga actcagaata tcgtctcaaa tgcttttttt
    10921 gaaaggattt atagttatga ctaaaacact tcctaaagat tttatttttg gtggtgctac
    10981 agctgcttat caagcagaag gtgcgactca cacggatggt aaaggcccag ttgcatggga
    11041 taagtatctt gaagacaatt attggtacac agcagagcct gcaagtgatt tttaccataa
    11101 atatcctgtt gatttagaat tagctgaaaa atatggcgtt aatggtattc gtatttcaat
    11161 tgcctggtct cgtattttcc caactggcta tggcgaagta aatgaaaaag gtgttgaatt
    11221 ctaccataat ctttttgccg agtgccacaa acgtcatgtt gagccattcg tgactttgca
    11281 ccactttgac acaccagaag ttcttcactc agatggagat ttcttaaacc gcgaaaatat
    11341 agaacacttt gtagattatg ccgctttctg ttttgaagag ttcccagaag taaactactg
    11401 gacaactttc aatgaaattg gcccaattgg agatggtcaa tacttggttg gtaaattccc
    11461 tccaggcatt caatatgatc ttgccaaagt cttccaatca caccacaata tgatggtttc
    11521 tcatgcacgt gctgtgaaat tgtataaaga taaaggttat aaaggcgaaa ttggtgtagt
    11581 acatgcgttg ccaaccaaat acccttatga tccagaaaat ccagcagatg ttcgtgccgc
    11641 tgagttagaa gatatcatcc ataacaaatt tatcttggat gcaacctacc ttggacacta
    11701 ttcagataaa acgatggaag gggtcaacca catcctagct gaaaatggtg gagaacttga
    11761 tcttcgtgat gaagacttcc aagcacttga agcagctaaa gatttgaacg atttccttgg
    11821 tatcaactac tacatgagtg attggatgca agcctttgat ggtgagactg aaatcattca
    11881 caatggtaag ggtgaaaaag gaagttctaa gtaccagatt aaaggtgttg gtcgccgaat
    11941 cgcaccagat tatgtgccta gaacggattg ggactggatt atttatcctg aaggcttgta
    12001 tgaccaaatc atgcgagtga aaaatgatta tccaaactac aagaagattt acatcactga
    12061 aaacggtctt ggatacaaag atgagtttgt agataatacc gtttatgatg atggtcgtat
    12121 tgattacgtg aagaaacact tggaagtttt atcagacgca attgcggatg gtgcaaatgt
    12181 taaaggttac ttcatctggt cacttatgga tgtcttctca tggtcaaacg gatacgaaaa
    12241 acgttatgga ctattctatg tagactttga cacgcaagaa cgctatccta agaaatcagc
    12301 tcattggtat aagaaagtag cagaaactca agtgatagag taatattact tgaaaatgta
    12361 aaaaattgtt atccgatact ttagaaaaag tacaacactt atttagacca ggctttgaaa
    12421 aggtatatat tgttagtttc gaagattgtc ctatgattcc tgagcttgaa gctaccccgt
    12481 tgctaaaatg tggtaaatgg tatgtttcaa ctggaaaaga gtggatttgc cattctgatt
    12541 tagagttatc agcatttgaa tgggaatttt tacaatcact cgatgtagaa atacgtgaga
    12601 ctattcactt cgaggtaaat tatttgcctt tccaataata aaaagaaagg aattagttgt
    12661 gactatcgaa ttaaaaaacg agtatcttac tgttcagttt aaaacacttg gtggtcaact
    12721 gacttcgatt aaagacaagg atgggataga gtatctctgg caagctgatc caaattattg
    12781 gaatggacag gcgcctattc tatttcctat ctgtggtagt ttacgaaatg attgggctat
    12841 ttatagacct caagaaagac ccttttttac aggtcttatt cgtagacatg gttttgttcg
    12901 aaaagaagaa tttattctgg aagaagtcaa tgataatagc gtgaccttta gtattaaacc
    12961 aaatgctgag atgcttgata attacctata tcagtttgaa ctgagagttg tttatacctt
    13021 gaatggtaaa tcaattagaa ccgaatttca ggtaacgaac ttggaaatgg aaaaaacaat
    13081 gccttacttt attggagccc atccagcatt caatagtcct ttagtagaag gtgaaaacta
    13141 tgaggattat tatattgaat ttagtgaggt tgagtcttgc tcgataccaa agagttttcc
    13201 tgagacagga ttattagatt tgcaagatcg gacacctttt ttggaaaatc aaaaaattct
    13261 cgatttggac tactcacttt tttcccatga tgccattaca cttgaccagc tcaagtctcg
    13321 tagcgtaacg cttcggtcaa ggaagtctgg taaaggtctt cgagtagatt ttgatgattt
    13381 tccgaacttg attctgtggt ctacaactaa taaaagtcca ttcattgctt tggaaccttg
    13441 gagtggtctc tccacttcac ttgaagaagg taatttctta gaagataaaa gacaggtaac
    13501 gaaaatttca cctcaagaaa cttctagaaa aatctatgat attactatcc ttaattaaaa
    13561 tttgttatta tttgagtctt caataagatt tgaatactct taccttgtga tgatagattg
    13621 gtcttctccg tgccagtctt acatcgcaag gtttttaagt tttttgtggg tctatgtcaa
    13681 ctgtagtggg tgacgaaaag ctaatatcta aagacagtct ggtagtaaaa aataagatta
    13741 tttactactt cttgatttaa aataatagtg gagccattaa tttgtggcaa atttaattta
    13801 atattttgcc tcaaattagc cacacttttt atagaactaa gaggaaaaat gaaattttct
    13861 tcctattaat atagttgttt gaaatcttaa atatagcg
//
LOCUS       DAJSVP010000064        13747 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSVP010000064 DAJSVP010000000
VERSION     DAJSVP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.066"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..863
                     /locus_tag="ON449_001918"
     CDS             <1..863
                     /locus_tag="ON449_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3934497.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="ON449_001919"
     CDS             1189..2040
                     /locus_tag="ON449_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3934498.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="ON449_001920"
     CDS             2137..4017
                     /locus_tag="ON449_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3934499.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="ON449_001921"
     CDS             complement(4057..4959)
                     /locus_tag="ON449_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3934500.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="ON449_001922"
     CDS             5190..6593
                     /locus_tag="ON449_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3934501.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /locus_tag="ON449_001923"
     CDS             6593..8026
                     /locus_tag="ON449_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3934502.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="ON449_001924"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="ON449_001924"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3934503.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="ON449_001925"
     CDS             9311..11038
                     /locus_tag="ON449_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3934504.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11053..11403
                     /locus_tag="ON449_001926"
     CDS             11053..11403
                     /locus_tag="ON449_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3934505.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="ON449_001927"
     CDS             complement(11495..12355)
                     /locus_tag="ON449_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3934506.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="ON449_001928"
     CDS             12447..12875
                     /locus_tag="ON449_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3934507.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="ON449_001929"
     CDS             13037..13213
                     /locus_tag="ON449_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3934508.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="ON449_001930"
     CDS             13240..13686
                     /locus_tag="ON449_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3934509.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DAJSVP010000065        13344 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSVP010000065 DAJSVP010000000
VERSION     DAJSVP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.067"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON449_001931"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON449_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3934510.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON449_001932"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON449_001932"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3934511.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ON449_001933"
     CDS             complement(2169..2906)
                     /locus_tag="ON449_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3934512.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ON449_001934"
     CDS             complement(2997..3524)
                     /locus_tag="ON449_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934513.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ON449_001935"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ON449_001935"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3934514.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ON449_001936"
     CDS             complement(4401..4886)
                     /locus_tag="ON449_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3934515.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ON449_001937"
     CDS             complement(5039..5236)
                     /locus_tag="ON449_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934516.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON449_001938"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON449_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3934517.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON449_001939"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON449_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3934518.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ON449_001940"
     CDS             9211..9423
                     /locus_tag="ON449_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3934519.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ON449_001941"
     CDS             9425..11104
                     /locus_tag="ON449_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3934520.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ON449_001942"
     CDS             complement(11358..11558)
                     /locus_tag="ON449_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3934521.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="ON449_001943"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="ON449_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="ON449_001944"
     tRNA            complement(12249..12322)
                     /locus_tag="ON449_001944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="ON449_001945"
     CDS             complement(12634..13032)
                     /locus_tag="ON449_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934522.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..>13344)
                     /locus_tag="ON449_001946"
     CDS             complement(13025..>13344)
                     /locus_tag="ON449_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934523.1"
                     /translation="MLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTI
                     KVAGGPIKYDRIENKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVY
                     VHG"
BASE COUNT         3929 a         2839 c         2272 g         4304 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac attt
//
LOCUS       DAJSVP010000066        13204 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSVP010000066 DAJSVP010000000
VERSION     DAJSVP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.069"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            628..1161
                     /locus_tag="ON449_001947"
     CDS             628..1161
                     /locus_tag="ON449_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3934524.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1651..2175)
                     /locus_tag="ON449_001948"
     CDS             complement(1651..2175)
                     /locus_tag="ON449_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934525.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="ON449_001949"
     CDS             complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="ON449_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3934526.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2764..3744)
                     /gene="pta"
                     /locus_tag="ON449_001950"
     CDS             complement(2764..3744)
                     /gene="pta"
                     /locus_tag="ON449_001950"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3934527.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3897..4577)
                     /locus_tag="ON449_001951"
     CDS             complement(3897..4577)
                     /locus_tag="ON449_001951"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3934528.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4703..5572
                     /locus_tag="ON449_001952"
     CDS             4703..5572
                     /locus_tag="ON449_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934529.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5598..6113
                     /locus_tag="ON449_001953"
     CDS             5598..6113
                     /locus_tag="ON449_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934530.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6160..6753)
                     /locus_tag="ON449_001954"
     CDS             complement(6160..6753)
                     /locus_tag="ON449_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934531.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6778..6957)
                     /locus_tag="ON449_001955"
     CDS             complement(6778..6957)
                     /locus_tag="ON449_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934532.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(7034..7543)
                     /locus_tag="ON449_001956"
     CDS             complement(7034..7543)
                     /locus_tag="ON449_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934533.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7639..8019)
                     /locus_tag="ON449_001957"
     CDS             complement(7639..8019)
                     /locus_tag="ON449_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3934534.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8349..9098
                     /locus_tag="ON449_001958"
     CDS             8349..9098
                     /locus_tag="ON449_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3934535.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9282..10559)
                     /locus_tag="ON449_001959"
     CDS             complement(9282..10559)
                     /locus_tag="ON449_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934536.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="ON449_001960"
     CDS             complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="ON449_001960"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3934537.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11481..12425)
                     /locus_tag="ON449_001961"
     CDS             complement(11481..12425)
                     /locus_tag="ON449_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3934538.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12427..13113)
                     /locus_tag="ON449_001962"
     CDS             complement(12427..13113)
                     /locus_tag="ON449_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3934539.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3826 a         2750 c         2342 g         4286 t
ORIGIN      
        1 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
       61 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
      181 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
      241 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
      301 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
      361 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
      421 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
      481 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
      541 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
      601 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
      661 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
      721 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
      781 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
      841 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
      901 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
      961 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     1021 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     1081 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     1141 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     1201 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     1261 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     1321 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     1381 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     1441 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     1501 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     1561 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     1621 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     1681 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     1741 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     1801 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     1861 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     1921 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     1981 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     2041 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     2101 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     2161 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     2221 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     2281 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     2341 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     2401 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     2461 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     2521 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     2581 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     2641 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     2701 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     2761 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     2821 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     2881 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     2941 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     3001 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     3061 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     3121 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     3181 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     3241 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     3301 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     3361 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     3421 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     3481 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     3541 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     3601 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     3661 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     3721 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     3781 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     3841 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     3901 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     3961 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     4021 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     4081 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     4141 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     4201 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     4261 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     4321 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     4381 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     4441 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     4501 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     4561 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     4621 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     4681 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     4741 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     4801 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     4861 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     4921 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     4981 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     5041 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     5101 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     5161 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     5221 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     5281 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     5341 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     5401 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     5461 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     5521 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     5581 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     5641 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     5701 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     5761 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     5821 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     5881 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     5941 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     6001 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     6061 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     6121 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     6181 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     6241 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     6301 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     6361 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     6421 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     6481 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     6541 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     6601 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     6661 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     6721 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     6781 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
     6841 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     6901 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     6961 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     7021 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     7081 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     7141 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     7201 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     7261 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     7321 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     7381 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     7441 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     7501 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     7561 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     7621 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     7681 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
     7741 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     7801 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     7861 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     7921 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     7981 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
     8041 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     8101 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
     8161 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
     8221 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
     8281 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
     8341 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
     8401 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
     8461 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
     8521 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
     8581 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
     8641 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
     8701 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
     8761 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
     8821 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
     8881 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
     8941 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
     9001 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
     9061 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
     9121 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
     9181 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     9241 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
     9301 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
     9361 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
     9421 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
     9481 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
     9541 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
     9601 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
     9661 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
     9721 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
     9781 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
     9841 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
     9901 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
     9961 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    10021 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    10081 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    10141 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    10201 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    10261 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    10321 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    10381 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    10441 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    10501 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    10561 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    10621 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    10681 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    10741 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    10801 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    10861 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    10921 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    10981 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    11041 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    11101 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    11161 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    11221 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    11281 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    11341 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    11401 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    11461 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    11521 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    11581 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    11641 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    11701 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    11761 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    11821 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    11881 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    11941 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    12001 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    12061 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    12121 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    12181 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    12241 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    12301 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    12361 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    12421 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    12481 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    12541 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    12601 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    12661 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    12721 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    12781 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    12841 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    12901 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    12961 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    13021 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    13081 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    13141 ttttttaatt ataaagcaaa acacataaaa aatatagttg ttttctcacg aaatagacac
    13201 tatt
//
LOCUS       DAJSVP010000067        12847 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSVP010000067 DAJSVP010000000
VERSION     DAJSVP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.070"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..305
                     /locus_tag="ON449_001963"
     CDS             6..305
                     /locus_tag="ON449_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934540.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ON449_001964"
     CDS             378..1739
                     /locus_tag="ON449_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3934541.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ON449_001965"
     CDS             1761..2021
                     /locus_tag="ON449_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934542.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ON449_001966"
     CDS             2035..2787
                     /locus_tag="ON449_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3934543.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ON449_001967"
     CDS             complement(2881..3486)
                     /locus_tag="ON449_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3934544.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ON449_001968"
     CDS             complement(3539..4513)
                     /locus_tag="ON449_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3934545.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ON449_001969"
     CDS             complement(4891..5610)
                     /locus_tag="ON449_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3934546.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON449_001970"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON449_001970"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3934547.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ON449_001971"
     CDS             7161..8558
                     /locus_tag="ON449_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3934548.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ON449_001972"
     CDS             8639..9079
                     /locus_tag="ON449_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3934549.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ON449_001973"
     CDS             9227..9553
                     /locus_tag="ON449_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3934550.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ON449_001974"
     CDS             9554..10303
                     /locus_tag="ON449_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934551.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ON449_001975"
     CDS             complement(10383..10838)
                     /locus_tag="ON449_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3934552.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ON449_001976"
     CDS             complement(11265..11540)
                     /locus_tag="ON449_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934553.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ON449_001977"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ON449_001977"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3934554.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4110 a         2214 c         2622 g         3901 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgttt
//
LOCUS       DAJSVP010000068        12308 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSVP010000068 DAJSVP010000000
VERSION     DAJSVP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.071"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..340)
                     /locus_tag="ON449_001978"
     CDS             complement(<1..340)
                     /locus_tag="ON449_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3934555.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            992..1963
                     /locus_tag="ON449_001979"
     CDS             992..1963
                     /locus_tag="ON449_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3934556.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2041..2277)
                     /locus_tag="ON449_001980"
     CDS             complement(2041..2277)
                     /locus_tag="ON449_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3934557.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2246..2377)
                     /locus_tag="ON449_001981"
     CDS             complement(2246..2377)
                     /locus_tag="ON449_001981"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3934558.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2453..2836)
                     /locus_tag="ON449_001982"
     CDS             complement(2453..2836)
                     /locus_tag="ON449_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3934559.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2869..3654)
                     /locus_tag="ON449_001983"
     CDS             complement(2869..3654)
                     /locus_tag="ON449_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3934560.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3832..4932
                     /locus_tag="ON449_001984"
     CDS             3832..4932
                     /locus_tag="ON449_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3934561.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4922..5746
                     /locus_tag="ON449_001985"
     CDS             4922..5746
                     /locus_tag="ON449_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3934562.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5743..6558
                     /locus_tag="ON449_001986"
     CDS             5743..6558
                     /locus_tag="ON449_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3934563.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6555..7385
                     /locus_tag="ON449_001987"
     CDS             6555..7385
                     /locus_tag="ON449_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934564.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7392..8423
                     /locus_tag="ON449_001988"
     CDS             7392..8423
                     /locus_tag="ON449_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934565.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8530..9803)
                     /locus_tag="ON449_001989"
                     /pseudo
     CDS             complement(8530..9803)
                     /locus_tag="ON449_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10030..10215
                     /locus_tag="ON449_001990"
     CDS             10030..10215
                     /locus_tag="ON449_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934566.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10339..11055
                     /locus_tag="ON449_001991"
     CDS             10339..11055
                     /locus_tag="ON449_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3934567.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10957..11477)
                     /locus_tag="ON449_001992"
                     /pseudo
     CDS             complement(10957..11477)
                     /locus_tag="ON449_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11765..>12308
                     /locus_tag="ON449_001993"
     CDS             11765..>12308
                     /locus_tag="ON449_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3934568.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEE"
BASE COUNT         3552 a         2277 c         2798 g         3681 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaag
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagcca gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1861 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1921 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1981 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     2041 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     2101 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     2161 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     2221 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     2281 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     2341 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2401 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2461 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2521 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2581 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2641 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2701 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2761 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2821 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2881 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2941 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     3001 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     3061 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     3121 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     3181 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     3241 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     3301 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3361 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3421 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3481 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3541 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3601 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3661 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3721 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3781 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3841 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3901 ctgacaggct gcgtctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3961 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     4021 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     4081 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     4141 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     4201 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     4261 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     4321 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4381 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4441 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4501 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4561 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4621 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4681 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4741 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4801 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4861 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4921 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4981 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     5041 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     5101 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     5161 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     5221 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     5281 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     5341 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5401 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5461 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5521 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5581 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5641 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5701 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5761 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5821 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5881 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5941 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     6001 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     6061 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     6121 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     6181 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     6241 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     6301 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6361 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6421 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6481 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6541 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6601 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6661 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6721 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6781 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6841 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6901 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6961 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     7021 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     7081 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     7141 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     7201 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     7261 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     7321 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7381 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7441 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7501 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7561 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7621 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7681 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7741 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7801 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7861 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7921 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7981 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     8041 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     8101 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     8161 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     8221 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     8281 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     8341 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8401 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8461 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8521 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8581 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8641 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cgcttttttt
     8701 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8761 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8821 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8881 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8941 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     9001 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     9061 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     9121 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     9181 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     9241 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     9301 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9361 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9421 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9481 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9541 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9601 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9661 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9721 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9781 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9841 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9901 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9961 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
    10021 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
    10081 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
    10141 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
    10201 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
    10261 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
    10321 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10381 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10441 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10501 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10561 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10621 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10681 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10741 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10801 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10861 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10921 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10981 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    11041 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    11101 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    11161 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    11221 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    11281 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    11341 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11401 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11461 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11521 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11581 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11641 gtctgtttgc atttattaaa aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    11701 tagatattat agagccataa tatgctgtat aatatctagt gtataggctt aagtggaggt
    11761 atatatgaaa atattaactg ttgaagatga taatatgata agagagggaa taagtgaata
    11821 tctttcagaa tttggatata ctgttattca agctaaagat ggaagagaag ccttgtcaaa
    11881 atttaatagc gatataaatt tggttatctt agacattcag atacctttta taaatggttt
    11941 agaagtgttg aaagaaatta gaaagaaaag caatttacca attctaatct tgactgcatt
    12001 tagtgatgaa gaatataaaa ttgatgcatt tactaattta gtagacggat atgtagaaaa
    12061 gccattttca ttgccggtct taaaagctag aatagattct ttaattaaga agaattatgg
    12121 acatttagag aagtttgaat ataaggatct atcggttaat tttaatagct atacagctaa
    12181 aataaatggt gaagaaatag atgtaaatgc aaaagaacta gaagtattaa aatgtttatt
    12241 ggataataat ggacaagtgt taacaagaat gcaaattatt gattatgtat ggaaagatag
    12301 cgaagaaa
//
LOCUS       DAJSVP010000069        12270 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSVP010000069 DAJSVP010000000
VERSION     DAJSVP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.072"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1065
                     /locus_tag="ON449_001994"
     CDS             <1..1065
                     /locus_tag="ON449_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3934569.1"
                     /translation="KKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNA
                     KEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSD
                     KVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGE
                     KELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIAT
                     QHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLR
                     KNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHP
                     SFSQPYNFILKAVTGLNIET"
     gene            1177..2628
                     /locus_tag="ON449_001995"
     CDS             1177..2628
                     /locus_tag="ON449_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3934570.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2977..3609
                     /locus_tag="ON449_001996"
     CDS             2977..3609
                     /locus_tag="ON449_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3934571.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4197..5636
                     /locus_tag="ON449_001997"
     CDS             4197..5636
                     /locus_tag="ON449_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3934572.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5931..6149
                     /locus_tag="ON449_001998"
                     /pseudo
     CDS             5931..6149
                     /locus_tag="ON449_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6398..6613
                     /locus_tag="ON449_001999"
     CDS             6398..6613
                     /locus_tag="ON449_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934573.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6830..7086)
                     /locus_tag="ON449_002000"
                     /pseudo
     CDS             complement(6830..7086)
                     /locus_tag="ON449_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7273..8184)
                     /locus_tag="ON449_002001"
     CDS             complement(7273..8184)
                     /locus_tag="ON449_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3934574.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8203..9207)
                     /locus_tag="ON449_002002"
     CDS             complement(8203..9207)
                     /locus_tag="ON449_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3934575.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9204..11327)
                     /locus_tag="ON449_002003"
     CDS             complement(9204..11327)
                     /locus_tag="ON449_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3934576.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11332..12252)
                     /locus_tag="ON449_002004"
     CDS             complement(11332..12252)
                     /locus_tag="ON449_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3934577.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3877 a         2243 c         2265 g         3885 t
ORIGIN      
        1 aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc tacaggttca
       61 aagcaaatta tgctagatat accggggaat aatttagcta atattttttc gataaagaat
      121 tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa aaaagtagga
      181 attattggag caggatatat tggagttgaa attgctgaag ccataagaaa aagagggaaa
      241 gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga ccgtagtttt
      301 tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct atcagaaagt
      361 cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag tggtacccaa
      421 tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc aattggggaa
      481 aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca gcaaacaaat
      541 ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc cattaataga
      601 agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc aactcaacat
      661 attaacaatc aagattttac accttctggt tcccaattta caaatggctt ttctatattt
      721 aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt aagtgagtct
      781 atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa aaatagtcgt
      841 gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt
      901 agtcaagaag atttatctgg actattatca atgttttctc ttgctataga agaacaaatt
      961 acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat
     1021 aattttattt taaaggcagt aacaggatta aacattgaaa cataatcgtt actaaatgaa
     1081 atcaagaaat atactgtatt actcaattca ttgaaaatat ttatcataat ttttaataga
     1141 aaacactatt aaagagaaaa aagagaggta aatcaaatga aaaaatcttt aaaacttgca
     1201 tcagcagtta tattaatgtc ccaactcctt atagcaacac ctttccatgt gttggcaagc
     1261 gaaaattcaa catacaaaat tgcaaaagaa agaaccgaga cgactgaaat gagtaattcc
     1321 gatgtaacag cagagagtag tgaacttttg acaacttcat caagtgaaag tagtacgact
     1381 tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc aaatgccgag
     1441 tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact
     1501 tcgtcaagtg aaagtagtac gatttcaagt accgaatctt caacaacttc aagtagtaca
     1561 agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata tagaatcttc aaaaaaggaa
     1621 gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac cacagcaaat agaatcaaca
     1681 gaatctttcc aatcacaacc aatactatct ggtaatgaag aaattcattt tgacaaaaat
     1741 gaatctgtag aaaattttat tatgaaaatt ggtgagtctg cgagaaaaat tggtcagaaa
     1801 tatgatttat atgcttctgt tatgattgca caagcgatct tagaatctgc cagtggtcaa
     1861 agccagttag ctcaggcacc gaattacaat ttatttggga taaaaggaac ttataatgga
     1921 aactttgtca ttatggtaac aaacgaagat ttgggtaatg gaacgcttta tacgacccaa
     1981 tcaaaattta gagtgtatga aaactatgag gaaagctttg aagattatgc caaattatta
     2041 actaagggaa ttagtggaaa taaagatttt tatgcaggag cattaaaagc taatagtaaa
     2101 acatatcgag aagcaactaa attccttaca ggacggtatg caacagatac acaatactat
     2161 ttgaagctaa atgaattaat taaaacctac gatttaacca actatgataa agaagttaaa
     2221 ggtccacaaa tgagtaagac tggataccaa gtgcctattc ataattattc aataaccagt
     2281 ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg atatggcagc tacacagggt
     2341 gagccgattc atgcagttaa agctggtaag gttgttaaag cagagtttca ctactcttgg
     2401 ggaaattacg tggtgataca acacgaagat ggatcaaccg cgttatatgc tcaccagcaa
     2461 caatacctag taaaaactgg agataatgtt ttgcaaggac aaataattgg ctatgtagga
     2521 agtactggga atagtactgg cccacacctt catttggaaa tttgcaaaga tagtagtctt
     2581 tctcagagca tgttagttga tccgaaaacc ttattattcg gagactaaaa aaggtaatca
     2641 atctgacaaa gtaacaagaa aatggttgga ttcttagacc tttgatcagg atattgagac
     2701 tggaattgat atctcgatta cgatgggttt gacaaataaa acaagtccat tatcgaatat
     2761 cgagtgtatt attgccgtct ttatgctcat attttgagca ggtttcacta gatgaaaaca
     2821 atttatctat cttgaggatt tcgtgtacgt atcgttcagt cttgtattgt aaattagtca
     2881 taaaatcaga ctaagaaaaa ttggaaatat ttttttctga tttattaaaa gttaattgaa
     2941 attatatggt acttctttct tgggagggta ggtgcagtga aggaaagata tggtcacctt
     3001 actgttgttg aggagattac tcataatgga tataaaaaat atcgttgcct atgtgactgt
     3061 gggaatgaaa cgattgtata tcgatcaaac ttactatcag gtagaaccaa aagttgtgga
     3121 tgtggaaatt ataaaaatag gcataaatat aaaaatttga caaataaaac ttttggtaag
     3181 ttagttgcaa tcaagccaac taaactgcga agtgaaggaa ctgttgtttg ggaatgtcaa
     3241 tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac ttctaaaagg tgatgtaaaa
     3301 agctgtggat gtttgaaatc aaaaaagcat cacattgaaa ataaaaaatt cggattactt
     3361 actgcaaagt atcctttgga caataactgt tcatggaaaa caaaatgggt ttgtcattgt
     3421 gagtgtggca gtatgtgtga gataagttat agtaacttat tttttggaca tactcaaagt
     3481 tgtggatgtt taaaaaggca agaaacaagg acatgtgttg aagggacagt aatagagtgt
     3541 ctaacgtcaa aattaccgaa aaataataca agtggtgtta agggagttta tcgttctaaa
     3601 gagaaataga ttgcgtatat aactttaaaa aaaaagaggt actatttagg atcgtatgat
     3661 tctattaaga gtgcagctca agctagaaaa gatgctgaaa ataaattgtt tcaacctttg
     3721 attgacaaat atcgaggtaa tcattaatta ttgtggataa taattaacag tgatatataa
     3781 acatctataa tatattattc atactattga tatttagtat aatcctatgg aattgaatat
     3841 catagcaatg tatttgaaaa gcaaattgga aaacaaatag gtgcaacacc ataaatatga
     3901 aaaaattggg aaaaagtaga atggaaattt gcttacgtct cacgagatag ctcacactat
     3961 taatctgaag aacaatgaag tcgactttta gaaattgcta gaagaaagta aagcgaatta
     4021 tcactagtta ctatagagta agttcaaata aagcatctcc aacttacatg gttacatgag
     4081 gatatcaaat cgaaattact caagcttcct atggctagag tcgcgagaag ttgttgggca
     4141 gtagacatag gttatattgt tcttagctac acgatcatta gaaaggaaat cctattatgt
     4201 atagttatca agaagatgca aaagtccaaa aaaatcgctg gtggattttg attgctatat
     4261 ctatgtttac cttcatgtcc acattggatg gcagtattgt caatattgca ctacctacca
     4321 tctcagatga tatgggcata ccaatgaatc aagcggaatg ggtagtgtcc gtttatctga
     4381 tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg cgatagccgt gggaaaatca
     4441 aagtatttcg gattggtacg ctgatctttg tcttgggatc gctgttgtgt gggttcaatc
     4501 attcgctagg tattttattg ttcgcacggg tggtacaagc cattggtgct agtatgacta
     4561 tggctacaaa tgccggaatc atcacggaag tctttcccat acatgaacgt ggtcgggcat
     4621 tgggagcaat aggtgcgttt gtttcattag gctcgattgc tggaccaggt cttggcggct
     4681 tgattttgtc ccaattttct tgggcgtaca ttttttggat caatgtaccg gtgggaatcg
     4741 taacgatgtt gattgctgaa aaattcttac caaaagatat caccatgagc ggccatacaa
     4801 tcgatggagg gggctttgct gccattgcgg ctacgattat cagctttttt ggagcaatct
     4861 ttttggggca agaaagtggc tttttccatc cagtccccct tgtactgttc gtaatttcag
     4921 tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc agcctctcca ttgatttctt
     4981 tcggtatttt taagaacaag atttttacca tgagcttgat tacagccttg ttgatttttt
     5041 cttccaactt ctttgtcaat gtagtgattc ctttttatct gcaaaatgct cgcgggttga
     5101 gtcctagcta tgctgggctg ttgatgatgg tctttccttt gatgatggtc gtcggggcgc
     5161 ctttaagcgg ctacctgacc gaccgggttg gcccggagat tttggtatta gtaggcttgg
     5221 gcttattgtc ggtaacccag gtcatgtata tgtttttgtc agtggactct cctttgtggc
     5281 tatacattgt cgctactgcc atcatgggtg ccggcaatgc cttgttccag tcccccaata
     5341 atacgatggt tatgagctcg gtcactatcg aaaacttggg actggcaggc tcgatgaatt
     5401 cctttgcccg aaatttgggg atggtcagcg ggatttcgct ttctacgacg gtcttatatc
     5461 aagcgatgtc tgctaagatg ggccagcaag taactaacta tatcgcaaat cggccggatg
     5521 tctttttata tggaatgaag attacctttt taggcagctt tgttttgtgt tttgttgcct
     5581 ttttcctgac tatctggcgt atcaaaaaac aaggaggtat tcagaaaaat gtctgaaaaa
     5641 accactgttg aagaattttt ggaggcctgc gtatgggtga gtgggatttc catggatgat
     5701 ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt attatcttca aaatactagt
     5761 gctcaatgag atgcttgatt tgatcacaag aagactgcga aagccgctgg caaccgcaag
     5821 ctaggtatcc caccgatgga ctacgtttta gaggctactg gttatgaata cggagccaac
     5881 gctctcatct gcataagaat acatcaccca gattatgtaa agattttcga tacttctgtc
     5941 aaaaaatcat taagatagat aaatgatttc cgtctagtca aattggttca gaatgtagac
     6001 acacagatgg taagaagtca ctcacaattc gagaatggac ttgtcctatt tgccgtatac
     6061 atcataatcg aaacgtcaac gatagtatca atattttgac caagggctat gaatacaagc
     6121 attgccttag aaaacatcaa gaaccttagg aacaacgggg atagcttggt taataagaga
     6181 taccgctgtt taggcttgca agtatactct tttatcaaga attccgtggc tttagtcatg
     6241 agaggcttaa tactatgaaa attttcttta gcatttttga tttaggttcc ttttctaatt
     6301 ataataagat gaccactaca gtaacctact gaaatatata aacagccact gtagtaccat
     6361 tttcaaagta gtctatgaga ggagaatctt cattcttatg aacgctggaa ttgcggatat
     6421 gaatttaata aaaaaaaccc ttaatgattt tacttccaat tcaatatcaa aaggtactgg
     6481 tatcaattta agcacaataa aaaagctgaa aagtggtgaa agatcagtgg aaaagctgaa
     6541 tcttttagat gctataaaaa taacggaatt tgctatgaaa aatggaaaag ctgagattga
     6601 aatatggaga taattatcgt ctagtgaatt acttgccact tagctgaatt taacgatcct
     6661 tggtgcttga agggggagat tcttgggaat agagcatact tgcaagccta tgtctttact
     6721 aacaacgata tctcttatta accaagctat ccccgtagtt ctaaggcttt tacttttatc
     6781 taagcaataa tcgtattcaa aggaaatttt tgacccttag aaagtctcat tttatcagac
     6841 gtgagcttag gcattgctga attttgttaa ttcggtttta ggttgtgctt tgagcataat
     6901 atgaaagtat tctttatcac gatttcattc atctaaggta atgtgatgtg attctgaaat
     6961 tctttcaaag gcagtttttg cataagctga cattacatca tcaatgacat gtttacaatg
     7021 tttcactact aaaataaggg ggtaaccaag aagaaaaact gaatgattat ttgtatctaa
     7081 ttccatggta tatcaaccat ttcctatata gactgattat gacatggatt aaaataaaaa
     7141 ggcactatgt cttaattgtc ggttttcgag ttaccgaatg gcattcatca cccacctata
     7201 gaggatgggc gacttctgcc tgaaataagt tacactactt ataacttcta ataaaattag
     7261 attgagtcat tattatttaa catcaacgat tttccagttt ccattttttt gtaatactaa
     7321 tttgtactga aagtagctag tggtttttga tacattatca aggtatttta tctttgtact
     7381 cactagaaaa ttttttccgt tttgtagata aactggattc tctaactccg aaaactttaa
     7441 gtcaatattg attggtcgca tcgcaccatt ttcaacataa tactccaatt ctttttcaga
     7501 agctgatgga taaagtttaa aaaatgtttc taaaaaatcg gtgatttctt ccgttgtttt
     7561 ggcatcaatt tctgaatcgt tttctacttg ttttgcttca tattttgcct tgtctggctt
     7621 tccaatcatt gttggcaaac tagttactaa atgattcctg ttttcatctt caaagattgt
     7681 cactcgatat gcagaagaaa ttgattgcgt cttttttccc tctgtaatat gttgctccac
     7741 taaaaatgaa acgacaaatt cttccgagtg tttttcaaca tctagaattt ttacagattg
     7801 aacttcggaa ctcgtgggaa tatctgctcg aaccatatct tggattaaag cgagaccgtt
     7861 ttctgtaagg tactgttcga gattactcat tctcttttca attgcttctt tgttattttt
     7921 ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt tcaacgccag aagtattcac
     7981 taccttctct tttataacca ctttttcatg agttgtatgt tgatcaatgg cggtgaaatt
     8041 tttataaacg ccaaaagcaa gactacaaga caataaaagc caaagggcaa gcaccatttt
     8101 tcgatgctta cccacggata caactcgttc ttttttcttt tttgggctct tttccttttg
     8161 attccgttca atttttatct tcattctttt tcctcttttc tcttatttgt atcggccagc
     8221 tccgataaga tgctgccgcc aatagttttc cgttagattc gtccacccca ctggatctcc
     8281 tgcatgaaac atgcgattct cccctgcgta aatgcccaca tgggtcacat aagtaccggc
     8341 atcataggtt gaatgaaaga aaactaaatc acctggtttg gcatctttta gatcaatatg
     8401 agtcatggca tcatattgat cctgagccac ccgaggaagt ttcaggtcaa ctttggcata
     8461 acaccattgg gtcaagccac tacaatcaaa ccccgtttct ggacttgagc caccaaacac
     8521 ataggccgtt ccttcatact taaatgcctc atcaaaaatt tcttggacta ttttgtcatc
     8581 aaactgagtt gtagttaggt attgcttcac taataaacag taaaacatat tgccataatt
     8641 ataccgccac ccaccatttt ccttaatcgc gattgggttg gggtaatcga ctttttgacc
     8701 acctgatttt tcctttgaaa aagattcagc taaagcaaaa ctatgcttct tcccgttttt
     8761 cccgacatga tctagatacc cgccaccata gttataggcc tgaataatac tatcttgatc
     8821 acaccctagt tctttggaac gcttaaccaa agacgcaaaa tagagacagc cttgtttgat
     8881 ggatgcctcg gtacttaatg aattaggtgg taatccggct gactcagaac tttgcataac
     8941 gtcttccatt gtgccaccag attccacttg aataatcgcc aaaagaattg gaatatactc
     9001 tgttatatcc gattctttgg catacttttc caccattgca cggtgtttca atacttcttc
     9061 tgagacacta gttccgccag gagaagtgcc agaactacca tttccctcac tgtcttcacc
     9121 aaacaacaaa cccacacaca tcaaaatacc cataaaagag atggtcagaa aagttaatga
     9181 aataacaccc actttcttca agttcatttt ttctcacctg accttgattt tggacgatgg
     9241 ttcattcttt tttgtacttt ttgattggtc tgctttggca gagtggacct ctgtttcact
     9301 tgttggaaag aatttttctt acttgctgat tggcttttcc cttttgaaat gatttcttga
     9361 ctagttagtt ttcgtttgat ttcgggattc agatttgctc tcacaatccg ctttggtgct
     9421 tcttttttca gcatcatatc tttctgaggt tgagctgctt tatctattgg aatattcgcc
     9481 cttctttttc cctgacgatt tgttgcttca ctcagtatct tgcgtttttc gtccattttc
     9541 gcatttcttc gttgtcgttg tttttctcgc aaatccactc gatccgcttt ttcttggaac
     9601 agtccgcgct tgaattcttc tggtgctttt ttcgactttt cgattccttt atggagattg
     9661 tatttcaaat tcgttggcgt attccttacc tgatccttta cttgtttagt cttatctttc
     9721 accaaagctt gggtatccaa aactttgccg atttttctcc cagccaaatt cattcgggat
     9781 tgtttcttat tatttgaaac tggattctcc ttagttaagt cagatagtat ttcgtgatcg
     9841 ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg gcttaaattt ggattttgat
     9901 tgttcctttg ccaacttatt tccgactgct gcaccaccac caaaagccaa agctcgtccc
     9961 acatttcgat tgagttgccg caattttcga ttcattaaca tttgcggttt tcgcatgacg
    10021 cgtcttccca ttgactggct atcattactc tggaggttga acatactcat aatgtcgccc
    10081 aatttgaaat agataccggc aaaggtcaca atttgcagaa aagcaaccat gaaaaatgga
    10141 taggttgccg aaatattgaa aaacatcgtg gagatgctaa atgcagtcgt aatcaccaaa
    10201 gtaacacccg ctcgcatcat aatcgtattg aataaacgaa taatcgcctt ctttcctaaa
    10261 ctttcataag tcggcaacat ggacaataag aaactaattg gcagaaacat cgcaaagata
    10321 atgaagagaa tttgcgagaa caacataatc cctgttagaa gaaaaacaaa gaaggaaatg
    10381 ccaatattga acaataccaa aaagaccacg gtccctaaac ggttcatggt cttggtaata
    10441 ctgagattcg cattgtcatt gtcttcaatc tctgccttga ccgcttcttc ccgatctttg
    10501 cctttgtttt catctggact cacagataga atcttgttga cccggtcttc accaatttct
    10561 tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt gttggacttg aatcgaaaac
    10621 aaactatccc gaatcaaatc cacactatct tttccttggc tttctgaatt gggtacaacg
    10681 atctcggttc caagggtaag ggccgcttca ctaacatctg agctaaagtc attgattttt
    10741 gtgatatagg taggcgcata agcaatgaac gaacccgata gtagaaaaat catcacaaaa
    10801 ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta ataatccggt atacgccaca
    10861 tacaccccca gagctaaaat catcaataat agaaacccaa aataaaatcc ggaggtctgg
    10921 agtccatttt cagtaatacc agccaatgtt tggatattct ttccaatact ttcagccgta
    10981 tccgaaatga aatctaattt gtaagcctct tgaaccacat aacccgtggc gttcgacaaa
    11041 tacaaactca ccgtccaaat gaaattagta atggcataaa gaccatacat cacgcttttc
    11101 cctaagccat caccccaatt ccaaggcaac caatcccaag agctatccac aaagaaatct
    11161 agttgatagt tgttcagtga atatttggaa tatagatttc ctgcttcaac tgtatcatca
    11221 actagccctg tcgcctcggc taccgtacca actaaactca atagaactat cagagtgagt
    11281 atgctaataa aaacgattcc cacaatccga aaaagtttct ttttcatccc ctcactccat
    11341 tctctcttga acaggtggac gggtatcaaa ggcatggaag agttcttcaa agactggatg
    11401 tacctgaacc acgcctaccc gaccatacaa atcctgcatc aagcattggc cattttccaa
    11461 atctcgtagg cgtttttgat tgctttcatc ttctcgatcc aagccaaaga attccaaggt
    11521 tttcttgatt tcatgaatat cggtactgcg aaacgcaaac ttcaacccga tattattctt
    11581 caagcgttca tccagtaaat catcactatt ttgcgtgaca aagtaaaccc cggcattcat
    11641 agcccgccca gcacgaacga gcttattcga taatgttttc ccttgcgcta cttgaagaaa
    11701 actccacgcc tcatctaagt ctacaatttt gaagatttct cgattactat ggataaagtc
    11761 caaggcaaaa gtggaaatga cgattagcat ggaaacacta agcaattcca tggtggtata
    11821 ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc gctacttgaa tgatatttaa
    11881 ctgcctatct aaactaatcg actgggtaat cgaaccatct gagaaaagca aatgggcaaa
    11941 atcataatcg gtaaaggact cgatgtgatc cgcgatattt tccgcaactg gtgagccttc
    12001 ttttcgaagc tcattaatta cttgtaataa tccccgttga ttgctttggg taaccgtccg
    12061 aatggcctta cgcaacactg gaaacttctc cccatctcga ctagaaattc ctgttaaaaa
    12121 ggtcagaata tcaattgcta gactttcaga atccttcttt ttctccataa tcacataagg
    12181 atcaagtaat ccttgatttt ttggcttact agttaagttg agaatattga tttcatccgc
    12241 aatttctggc aaggtttctt tccagccacc
//
LOCUS       DAJSVP010000070        12202 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSVP010000070 DAJSVP010000000
VERSION     DAJSVP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.073"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(164..328)
                     /locus_tag="ON449_002005"
     CDS             complement(164..328)
                     /locus_tag="ON449_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934578.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            623..1951
                     /locus_tag="ON449_002006"
     CDS             623..1951
                     /locus_tag="ON449_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3934579.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2153..2986
                     /locus_tag="ON449_002007"
     CDS             2153..2986
                     /locus_tag="ON449_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3934580.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3002..3739
                     /locus_tag="ON449_002008"
     CDS             3002..3739
                     /locus_tag="ON449_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3934581.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3894..4862)
                     /locus_tag="ON449_002009"
     CDS             complement(3894..4862)
                     /locus_tag="ON449_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3934582.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5094..5927
                     /locus_tag="ON449_002010"
     CDS             5094..5927
                     /locus_tag="ON449_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3934583.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5961..6800
                     /locus_tag="ON449_002011"
     CDS             5961..6800
                     /locus_tag="ON449_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934584.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6826..7386
                     /locus_tag="ON449_002012"
     CDS             6826..7386
                     /locus_tag="ON449_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3934585.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7585..9306
                     /locus_tag="ON449_002013"
     CDS             7585..9306
                     /locus_tag="ON449_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3934586.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9471..10613
                     /locus_tag="ON449_002014"
     CDS             9471..10613
                     /locus_tag="ON449_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3934587.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10629..11840
                     /gene="thiI"
                     /locus_tag="ON449_002015"
     CDS             10629..11840
                     /gene="thiI"
                     /locus_tag="ON449_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3934588.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2048 c         2536 g         3463 t
ORIGIN      
        1 cgtaggttcg agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa
       61 tcacttgggt ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg
      121 tatgttgtaa tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat
      181 catcgctaaa tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa
      241 gaaaagggat gacattccca aagttatctg tattcataag tatgccacca cccacaaaca
      301 aaaagccgcc agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt
      361 tccttctttc aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct
      421 tctttattat acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact
      481 attgtatacc gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa
      541 aatttcatca tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac
      601 ttggatttgg aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa
      661 caaacagttt caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg
      721 aattactgct tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat
      781 agatttggct acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc
      841 tgcactgaac acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt
      901 atcacaaaat tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa
      961 catcattgca acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac
     1021 tcctcaacaa gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt
     1081 tgtgcctaaa tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa
     1141 ctacttgaac aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg
     1201 agaaacatta gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt
     1261 cttagctatc tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga
     1321 aaagaactat catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg
     1381 tgtaaaatta gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa
     1441 tcgttcttat acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct
     1501 gaatgttgat atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc
     1561 tgtttggttt ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt
     1621 agatgcttat tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg
     1681 tttagatttt ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt
     1741 agacgggcaa tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa
     1801 tggcttcttt gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag
     1861 aaaagattta ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc
     1921 tccgtgggat ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta
     1981 aagataaaaa gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt
     2041 ttatggtccc tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc
     2101 attaaaaata tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat
     2161 ccaaactgtt ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga
     2221 tgttaatcag acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc
     2281 gaatgtaaaa aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt
     2341 cgtacgtgta ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat
     2401 ggaatggatc gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct
     2461 ccatcagcaa acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt
     2521 tcagaaaaat tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc
     2581 ccagatttct ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct
     2641 gcgatttaaa gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt
     2701 gcacggagac ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt
     2761 cgacccagct gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg
     2821 tggtttccga ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg
     2881 gaaagaccga ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg
     2941 agaaagttac ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt
     3001 tttggcaaaa aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca
     3061 aacgatgaca tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga
     3121 ccagttggga agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa
     3181 ttattccatg gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca
     3241 agccatggag tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg
     3301 gaaagaatta gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat
     3361 gagcagtgtt aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa
     3421 gcgtcaggaa aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt
     3481 ttatgatatc gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga
     3541 cgtattagga tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga
     3601 taaagaagta gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa
     3661 tggtgtcaaa acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat
     3721 tatatggaaa aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag
     3781 aagaaattta cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga
     3841 agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt
     3901 tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt
     3961 aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt ttttagttct
     4021 tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca
     4081 agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg
     4141 agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct
     4201 tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca
     4261 gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta
     4321 gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg
     4381 gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta
     4441 tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca
     4501 cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca
     4561 atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc
     4621 actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga
     4681 gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc
     4741 ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc
     4801 gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc
     4861 attttacaga cttccttacg aataattttg catagccgtt aaatcttatt gtatactata
     4921 taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg
     4981 accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg
     5041 agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca
     5101 gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc
     5161 tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc
     5221 cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtag
     5281 tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag
     5341 aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt
     5401 tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact
     5461 tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt
     5521 cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt
     5581 ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg
     5641 tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag
     5701 caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc
     5761 aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt
     5821 ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa
     5881 atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat
     5941 tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg
     6001 tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa
     6061 acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcattg
     6121 atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc
     6181 gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag
     6241 atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga
     6301 tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc
     6361 cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt
     6421 atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt
     6481 cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt
     6541 atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa
     6601 atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg
     6661 agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac
     6721 atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac
     6781 acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca
     6841 ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc
     6901 acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt
     6961 gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg
     7021 cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat
     7081 ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt
     7141 tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg
     7201 aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct
     7261 gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag
     7321 attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa
     7381 gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc
     7441 gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca
     7501 cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc
     7561 atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca
     7621 atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa
     7681 gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa
     7741 gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa
     7801 tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt
     7861 ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt
     7921 gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta
     7981 aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc
     8041 tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt
     8101 gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac
     8161 acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat
     8221 gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc
     8281 cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta
     8341 ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca
     8401 ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct
     8461 gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta
     8521 aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa
     8581 ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc
     8641 cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt
     8701 ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc
     8761 ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa
     8821 ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc
     8881 agtttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa
     8941 ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga
     9001 gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta
     9061 gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat
     9121 gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat
     9181 ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag
     9241 cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta
     9301 gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg
     9361 tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac
     9421 cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt
     9481 ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca
     9541 gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt
     9601 tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct
     9661 attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa
     9721 aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga
     9781 cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg
     9841 gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg
     9901 ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt
     9961 tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac
    10021 cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc
    10081 atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac
    10141 tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg
    10201 tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga
    10261 cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt
    10321 tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg
    10381 gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct
    10441 gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc
    10501 tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc
    10561 aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga
    10621 gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa
    10681 agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact
    10741 ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag
    10801 acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa
    10861 gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag
    10921 aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg
    10981 aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc
    11041 atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg
    11101 gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg
    11161 gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca
    11221 tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac
    11281 aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc
    11341 attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag
    11401 gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta
    11461 ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt
    11521 tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga
    11581 caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta
    11641 tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc
    11701 aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag
    11761 cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag
    11821 aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa
    11881 acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc
    11941 tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa
    12001 aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc
    12061 ccatgaaata gcgaggttgt gacagaagtg ggcagcttca agaaataaga aggaatttac
    12121 gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg
    12181 gaacaccgtg tatcagaggt tg
//
LOCUS       DAJSVP010000071        12201 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSVP010000071 DAJSVP010000000
VERSION     DAJSVP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.074"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            182..3409
                     /gene="fss3"
                     /locus_tag="ON449_002016"
     CDS             182..3409
                     /gene="fss3"
                     /locus_tag="ON449_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3934589.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3522..3836
                     /locus_tag="ON449_002017"
     CDS             3522..3836
                     /locus_tag="ON449_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3934590.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3849..4223
                     /locus_tag="ON449_002018"
     CDS             3849..4223
                     /locus_tag="ON449_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3934591.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4233..4568
                     /locus_tag="ON449_002019"
     CDS             4233..4568
                     /locus_tag="ON449_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3934592.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4651..5991
                     /locus_tag="ON449_002020"
     CDS             4651..5991
                     /locus_tag="ON449_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3934593.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6069..6743
                     /locus_tag="ON449_002021"
     CDS             6069..6743
                     /locus_tag="ON449_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3934594.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6736..6900
                     /locus_tag="ON449_002022"
     CDS             6736..6900
                     /locus_tag="ON449_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934595.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6976..8118
                     /gene="dcm"
                     /locus_tag="ON449_002023"
                     /pseudo
     CDS             6976..8118
                     /gene="dcm"
                     /locus_tag="ON449_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8108..8398
                     /locus_tag="ON449_002024"
     CDS             8108..8398
                     /locus_tag="ON449_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934596.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8657..9841
                     /locus_tag="ON449_002025"
     CDS             8657..9841
                     /locus_tag="ON449_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3934597.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9838..9975
                     /locus_tag="ON449_002026"
     CDS             9838..9975
                     /locus_tag="ON449_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3934598.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            10721..>12201
                     /gene="ltrA"
                     /locus_tag="ON449_002027"
     CDS             10721..>12201
                     /gene="ltrA"
                     /locus_tag="ON449_002027"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3934599.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT         4171 a         2024 c         2715 g         3291 t
ORIGIN      
        1 tgttctaaaa tgtgttctaa tttaaaggca gtatatttcg tattttagat taaattttta
       61 ggctcattca ttctcaaatc cattctattc agtcaataaa aattttaaaa agactttata
      121 aacaaagatg ttcaagcgag catcttattt ttttgtgctt aatttgaaag gagaacattt
      181 catgtataaa aaacaaaaaa agaaattatg tgtattaact attttgagtt tggctgttca
      241 agcaattgtt ggagcaattg ctcctacgat tacttttgct gatgaaatta cccatccaca
      301 aacggtaacc gtagaactag atttagcaca tcaatatgct gttgaaggaa cttttagtga
      361 tggtcgtcca atgtcagaag tcactgttcc gcattatgca gtgtacaacg gctttaaaca
      421 agatattttc tgtattgaac caggagtccc gatttacaat gagttcactc ctggctatga
      481 gaaaaacccg ttaccagata tgtccgataa agccaaatta gtttccgttt tatggaaaaa
      541 agtaggtaca gatgcagata ctcatattgt ggcacagaag atgatctggc aagaagttaa
      601 tgggtatacc cttcactcaa taaaacgatc agatggtagt gccgtaaata tcgcagccat
      661 tgaggcaaaa ataaatcaag ccattgacga ttatcagaag aaaccaacct tccataatag
      721 tacggctaaa acagtattag ggcaatcgac tactgtgacg gatacgaata atctgaattt
      781 gtcagagttc gatgaggttg tggaaaatgc cgcaaacatt gattatcgtg tgaatggtaa
      841 tcagttgata atcaccccaa atgccaattc aaaagaaagt ggtgtgctga cgcttaagaa
      901 atctgctggt accggaacac ctgtcgccta taaaaaggct ggccaacaga ctttaatggc
      961 cggggcgatt gataaaccta atacctacac aatcaaaatt gatgtggaaa cggagggttc
     1021 tttaaagatc aaaaaagtcg ataaagaatc gggtgatgtt gtaccaggaa cggttttcca
     1081 tttagacttt ggaaaaactt tacctgcaaa agacgtgact actgacaaag aaggcattgc
     1141 tacattggat gagattcctc atggtacaaa agtaaccatt accgaaaaat cagtaccggc
     1201 gccttatacg attgatacca cccccatgac taccaccatt aaagcaggcg aaaccattta
     1261 cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt ctggataaaa gcggagttga
     1321 aacagggagt gatctttgga atgacaatta ttccttagct ggtaatacgt ttgccattcg
     1381 caaagacagt ccaaccggtg aaatcgttca agaaatgacc acggatgaaa acggtcatgc
     1441 ggaaacacca aaagagattg ctaatgcgtt ggaattggga acctattatg tgaccgaaac
     1501 taaagctagt catgggttcg tgaatacgtt caaacctgta aaagtcgaat tgaagtatgc
     1561 caatcaaacc gtagcccctg ttaccagtaa cgtaaaaggg caaaatcaag aagtcactgg
     1621 cgaaaccact ttgacgaaag aagacaaaga tactggggat aaagcacaag gtaaggcagt
     1681 ctttgagggt gctgaataca ccttgtttac tgctaaggat ggaaaagctg ttaaatggag
     1741 tgaggctttt aaaccagagt tagcgaaggg aacgaaaggt tctgatgaaa cggtcacttt
     1801 gactttagac gaaaagaacc aagttgccgt taaacattta gctatcaacg agtattattg
     1861 gcaagaaaca aaagcgcccg aaggatatag ccttgatgaa acaaagtatc ctgtatccat
     1921 caaaaaggtg gatgatagcg aaaaaaatgc cgtgattacc cgaaatgtta cggcaaaaga
     1981 acaagtgatt cgctttggct ttgatttctt taaatttgct ggatcagctg cgggcactac
     2041 cgaaactggc ttcaatgatc tgacctttaa agtgtcgcca ttggaaggga ccaatgaaat
     2101 cacaggtgcc gaagatgaag cgaccacagc ttataacgag caattagggt ttgatggtta
     2161 tggcaagttt gaaaatcttc cttatgggga ttatttactt gaagaagtag aagccccaga
     2221 aggttttcaa aagattacgc cattagaaat ccgttctact tttaaggaaa acaaagaaga
     2281 ctttgtgaag agtgagtatg tctttaccat taccgaacaa gaccaaaaac aaccaattaa
     2341 aacggtgacc gttccttacg agaagctgac gaacaaagca ttttctgtta gtttgaaccg
     2401 tttaatgctc tatgatttgc ctgaggaaga agatagttta acttctcttg cgacttggaa
     2461 agacggaaac aaagaattaa cttctctgga ttctaccgag ctagttgata aattgagtta
     2521 caatctacat gaaatcaaag aagactggta tgtggtagcg caagccattg atgtggatgc
     2581 gacaaaagct gcccaagaaa aagatgaaaa agccaaacca gtggtgattg cagaaacaag
     2641 cgcaacgttg gcgaacaaag agaaaacagg aacttggaaa attcagcata aattaaccgc
     2701 cgaacaagtt ttaaataaaa ctattgtctt attcaactat gtgtatgaaa ataaggaagc
     2761 ttttgaagca ggcgataagc cagtagcgaa ggatgttagc ttgaataacc aagcccaaac
     2821 tgtcagttgt acagtggaac accatgtttc tatccaaaca aaggcccacc tagaaaacgg
     2881 ttctcagacc tttactcatg gtgatgtggt ggatatgttt gatgatgtgt cgattactca
     2941 tgatgtactg gatggctcaa aagaagcttt cgaaacaatt ctgtatgctt tattaccgga
     3001 tggtacaaac aaagaaattt ggaaatctgg caagattgat tatgaagtaa atgataaaga
     3061 attcaccaaa actgtgcttg cgaaaaaagt agataccgga aaatatccag aaggaaccaa
     3121 gtttactttt gcggaaatca attacgataa agatgggaat atcaatggaa aacacaatga
     3181 agatttgaaa gaaaaatctc aaaccttaac accaaaagaa gttccaacca tactaagtac
     3241 gccgaaacaa ccggaaacac caactgttcc aagtggctct caagaatcta gtcccacagt
     3301 gaagaccttc cctcaaaccg gggagaaaaa ttccaatgtt ttactgttca ttggttttac
     3361 tttgattttt gcgacggcgg gctattattt ctggaatcgc cggaactaag gtgatgagat
     3421 gatacaagta aatcctaaga accaacgtgc gcgaccgcct cctaaaaaag tagtcgcgct
     3481 atttttgtat ccaaaactaa caatgaaaag aggaaaagaa gatggaattg aaatttgtcg
     3541 tgccagatat ggctgaaacc tttggaaagt taagttatgc cggtgaagga gaaattataa
     3601 cagaaggata tggacggaat actacggtga ttggtcgcag ttatcatttg tattccagta
     3661 agcaacgggc cgatgatatt gaggtggtag tagctgcgga agccggtgaa aaggattttg
     3721 atcaagacca accgctaaaa gccgtgaatc cccatttggt tgccaaaggc tatgagattg
     3781 agaatcgtgg gtttactgat tacgtgttgt atgttgatga tttagtgaaa gcataggagg
     3841 aaaagaagat gagattaaca gaaggtattg tcgtagattc aggtttaaca tttgggaagt
     3901 tgcgtttttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga acaattggta
     3961 atgaggtaaa agaacgaacc tataatttaa aatcctctgc acaagggcga atgattcaag
     4021 ttagtatccc agctaatgta cccttacgcg agtttgctta tgatgcagag gtagagttgg
     4081 tcaatccaat cgtggatacc gttgccaatt atgtttttcg agaaggaact accgttaatt
     4141 ggttcattaa ggcggatgat ttagtcttga agcggcagcc gaatcaagga aatcctacga
     4201 accaaaatga aagaaagaaa taggtgaaga ttatggaaat taatgttgaa catattttag
     4261 attgtctcga tcaatatgga aaaggcgagc tgactgagga acaactcgtt aaagcattaa
     4321 cctctgatga aaaaatgttt ctgattatac atcaaggttt acttggagaa aatagcgaca
     4381 gtgaagaaga ctctgatgta ctagactggt taggagaaga agagtctttc tttatagttg
     4441 tcgaagtaaa tgaaaactta tgtcggcagg cagaatccat tttggaagaa attggtgtgg
     4501 agatgccgga tgctattgaa ggctttttaa atcaattggt tgaaacgaaa caactcccct
     4561 cctcgtagca gtaaatgact agcataactt tttgaaattg cctaatgaaa tcctattggg
     4621 ctttttcgat tggaggtgga aaccatcaaa atgtacaaag gacatcgcat acgcgcagga
     4681 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     4741 atgagcgtct tttattttca agagtttaga caatttgata tttctaagct atcactttct
     4801 actattgaaa tcgtttggtc tataaaagat ttagtatgtc tgcttggaag tctcgctttt
     4861 tcaggagcta tgatattgct ttatattcac ttttttcttg atcattggcg aagcttgtgg
     4921 catcgacaaa agctagctcg gatgattcta gagaaccatt ggtatgaagt gaaacaaact
     4981 caaagtgaag gctttttcaa agatttgaat agtagccgaa ccagagagac tatcagttac
     5041 ttccccaaaa tctattatcg aatgaaagat ggattactgt ctattcgcgt tcaaatttca
     5101 cttgggaaat accaagatca gcttttaaaa ttggaaaaga agttagaaag cggactgtat
     5161 tgtgagttag tagaaaagga actcaaagac tcctatgtgg agtatacttt actgtatgat
     5221 atgatcgcca atcgaattgg gatagacgaa gtagtggcag aaaatgggac tctgcgattg
     5281 atgaaaaatc aagtatgggc ttatgattct ttaccccata tgttaatcgc tggtggcacg
     5341 ggtggaggga agacctattt cctcctcacc attatcgaag cactattgaa gtcagatgca
     5401 gaattgttta tcctcgatcc caaaaatgca gatttagctg atttaggtac ggtgatgcct
     5461 catgtttatt cacaaaagga agaaatttct gcttgtgtgg aagatttcta tgaacgtatg
     5521 atggctcgta gcaaggcaat gaaagaaatg tctaactaca aaacaggaga gaattatgcc
     5581 tatcttggac ttccacccaa ctttttaatc tttgatgaat acgtggctta tatggagatg
     5641 ttaacgacga aagaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttgggt
     5701 cgtcaatctg gtttctttct gattttggct tgccaaaggc cggatgcgaa atatttagga
     5761 gacggaatcc gcgatcaatt caactttcgc gtggctttgg gtcgtatgag tgaactcgga
     5821 tattccatga tgtttgggga ggtcgaaaaa aacttcttta tgaagcacat caaaggtcga
     5881 ggttatgtgg atacaggtgg cagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     5941 gggtatgatt tcttagattc aattagtaaa ttagaaaaga tgaatgattg agtgatcttc
     6001 aatactgact ttatacttca agtaacgtat aatactttta tatagtagga agaaagggga
     6061 taattaatat ggaaagtaat gattcttatt tagagtatgc aaaagcaaat aaggataagt
     6121 ttatttctta tattatcaaa gggaaatccc ccgaagttgg acgagacgca gtttttatgg
     6181 cagggagtcc tggggctgga aaatcagagg ttgccattgg attagctgag aattatgata
     6241 accatgtgat tattgatgcg gattactttc gcacccagtt tcctgattat aatggaaaaa
     6301 actctagtct ctttcaaaaa gcatcttctt ggttggtaga gcaggcattt agatatgtct
     6361 tagatcatgg ctattctttt attctggatg caacttttgc aatattaagt gctgaaaaaa
     6421 atatctcgcg ggcgttgaaa aaacattttc gagtgacgat tttctatgtt tatcaagacc
     6481 ctaaaatcgc ttggaaattt acacaagaac gtgaaattgc agagggacgt catgtgccga
     6541 aaaaggcatt tattaacgct tttttcaaat caagagagaa cattgcaaaa gtaaagagta
     6601 aacatccaga tgtattgctt catatcataa taaaagatta tacgaataat atttccgaag
     6661 ttcattatgc tacggataat ctagaattat ttttaccact agggtatagt aggaaagatt
     6721 tggaggttca attacatgac tgagaaagaa caagtaacga aaattgtaaa gaaatacaat
     6781 aagagcattg cggatttatc ggaaaatgct actgcaaaag agttcaaaac ggtgattaaa
     6841 tatgtagccg atcaggccaa tgaaaaacaa cggaaattag ttggattaaa taaaaaatag
     6901 taggacaaat taataatgta tgagccaaga gcaaaatgtt cttggctttt ttctttgcaa
     6961 tgaatgaggt aatgtatgag atttttagat ttattcgccg gtattggtgg ctttcgttta
     7021 ggtatggaac aggcgagcca tcattgtatc ggtttttgtg aaattgataa atttgctaga
     7081 cgtagttata aagcaattca tgatacaagt aaggaggtgg aaatgcatga catcacaagt
     7141 gtatcagatg agtttattca atccctcgga ccagtggaca tcctttgcgg cggatttccg
     7201 tgccaagctt tttcaattgc gggaaagcgg caaagatttt ccgatactcg aggtacttta
     7261 ttctttgaaa tcgctcggtt caccactctt ctccgaccta agcttttatt ccttgagaac
     7321 gtccgaggat tgctcaatca cgaaggaggg gctacgttcg agacgatcct ccgaacgctg
     7381 gatggattgg ggtatgacgt ggagtggtag gtgcttaact caaaggccta cattccccaa
     7441 agccgggagc ggatattcct tatcggacat tctcgagacg catgtaccga acaagtattt
     7501 cctatcattg gaccgtcttc aacatctgat caaaacatca gaaatttggt aaatatcaat
     7561 ccttccaatc gaggaatggg tggacaagtc tatggttcag atggcgtagc gccaacctta
     7621 acaggtgatg aaggaatcaa aatcgcactg ccagcaaggg atggaatctc tgtagcagga
     7681 ctattaccgg gaaagtttga acaaggtaat cgagtttatg aagttactgg aaccgccccc
     7741 accttgtcaa cgaaacaagg tggtacaaaa atcatgattc gaaaaaatga aacagaatat
     7801 caagaggtga agccagaaga cagtgtgaat ctggcttttc ctaattccac aagtcgccga
     7861 ggtcgtctgg gaaaacagag tgttcatacc ttactaacag gagaccaaca agcgatcgtc
     7921 acggatcagt atcaaattcg gaagctaaca cctagagagt gttggcgact tcagggcttt
     7981 cccgattggg cttttgatcg agattctcaa gtcaattcag atagtcagtt gtataagcaa
     8041 gccggaaact ctgtaacagt tccggtcatt tttgatattg ccagaagatt aaaagagttg
     8101 gatgtacatg gtttatgatg agttgattgg agaaatttat tgggtgattg gaaagattca
     8161 gtcagaccca gaattagaag aacaattgcg acggttaaaa tttgatattc gcaagaatgg
     8221 tgtcaaagtc gctggcgatc catatttaat gaatgaggag acggatgctc gaattgaaat
     8281 aaatcaagta attgcagaat ttgagcggat tgcggaccaa gcaaaagaac cagatattcg
     8341 tcaatacttg ttagaaatga aagcagagtt ggaagtaaat ggtattacag atgagtaacc
     8401 taaaccaaca caactgactg ttaggcgctg accgcggagg attcgcgaag cagaaatcct
     8461 ccgtggtgct gtgcctgttc atggacggcg ttagccgacc atgatgtctc acccccgtat
     8521 ctaacagggg ggtacaaaaa cacaaaaaat aagtaagcaa ccattaagaa tgcttgttgt
     8581 atcaaagata gtaagtaatt tgtataggtg ttaactcatt tgaaaaagtt aacaccttga
     8641 atttggaggt gagaacttgg cacaacggaa tttagattat cggctattaa aagaccgacg
     8701 caacgaatac ggcgtttcac aaaataaatt ggctacggct tgtggactta gtcggccgta
     8761 cttaaatcag atagaaaatg gtggcgtaac cgcatctata aaaaccatga ggaaaatttt
     8821 tgaccaactg gaaagcttta atcccgattt gcctttgacg ttactgtttg actatgtaag
     8881 gattcgcttt cccacaacgg atgcacgaaa aattattcaa gaaattctcc atttgaaatt
     8941 tgagtatatg ctccatgaag attatgcctt ttactcctat caagaacagt atgtcatggg
     9001 tgatattgtg gtgatgttgt ctcatgaaga agataaaggt gtgcttttag aattaaaggg
     9061 gcgaggttgc cgacaatttg aaactttttt actcgctcaa aagcgtagct ggtacgactt
     9121 tttcgaggat tgtctaaagg ccggtggcgt gatgaaacgc ttggacttgg caatcaatga
     9181 tcgagtgggg ctattggata tacctgagct aacgaaaaaa tgtcagaagg aagaatgtat
     9241 ctccttgttt cgtaccttta aaagttatcg ttccggggaa cttttaaagg ctgatgaaaa
     9301 ggacggcatg ggcaatacct tatatatcgg cagtctcaag agtgaggttt atttctgttt
     9361 gtatgaaaaa gactatgaac aatatatcaa gttagggatt ccattagacc aaactgagac
     9421 gaaaaatcgt tttgagattc gactgaaaaa tgaccgtgct tttcatgcga ttcaagattt
     9481 gttaaaaggt cgtagcattg agagcaccac gttttccatt atcaatcgtt acttgcgatt
     9541 tgcggataaa gtagaaggta aaaggcgaac caactggccc ttgaatgaac aatgggggcg
     9601 ttttattgga cggaatcgga aagagattca attaacttcc gagccaaaac cgtataccat
     9661 tgaacgaacc ttgaattggc taggccgaca agtcgcccct acgtggaaaa tggctaagga
     9721 gttggatcga ttgaatcgga caacctatat ccaggatatg gtacagaatg cacgcttatc
     9781 agaccgacat aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg
     9841 aaaagaggaa tcgtcatggg aattgtaaaa gatatttttt tggtatttgg tggcagtatg
     9901 cttggtgtta ctgtgatgtg cttgatgaat gcaagtgcag cagcggatcg agcaatggaa
     9961 aagaaaggaa agtgacaaga tgaatttcgg acaaaattta tgtgcgactc gttccttagt
    10021 gaaaagctaa ggcactaata aaagaacgaa aaagtttggt tattaggaga actgataatc
    10081 tgacaggtgg aatgatgaag taacgctctg aaacgcctgc cctgagcctc cgacaagcat
    10141 tgtatgagga aactcagaat gtttgaagct cggtaaagac ggctgaaaga taccgtaata
    10201 gttgatgaac atatatcttc tatcgaaaac tatccagagg tgggtagcgt atccaacagg
    10261 tcgggggtct ataaaatatc tatggttaga atgttctata aggaactgac gaaacagcga
    10321 atgtacgggt ctaaatttga actttgtaga aatgcaaagg tacgagaaat cgtagctggt
    10381 gtggcaaagt aagaatatag gttatgaaat gccatataac gttacaggcg gtatcaagcc
    10441 agcaggctta tagctagtga cctaaggata tatatgtata gataagatta tcggaacgag
    10501 gaaaggtatt gggttctcat caagagaata aactgacgaa gaacaataag cagttgaact
    10561 aatgctgaaa agcagagggc gaacctaaga aattcttgta atgagaatgg aggaatgccc
    10621 tcaagtcgtg tatatattag aaaagtatta tgttgtttca ataaggatta cgagtaagat
    10681 aaaaaagctc actcaatttc aagtaaagga gtgaagcctt atgcctaaaa ataaaaatga
    10741 aacattgtgt gtagaagatt taagacacgc tgaatattac gaaatgcaaa acacctttga
    10801 tgatttatat gccaaaagta agaatggaga tattttcact catcttatgg atattatcct
    10861 atcgagagaa aatatattac tggcatatcg aaatatcaaa gcaaatgcag gcagtaaaac
    10921 agcaggaaca gacggtacaa taattaaaga tattggcaaa ttgccagcag aaacagttgt
    10981 caaaaaagta agatacattg ttgcaggcag tccacatggg tatcgaccta agccagtgag
    11041 gcgaaaagaa ataccaaaac caaatggtaa aaccagacct ctaggtattc cttgtatgtg
    11101 ggacaggctt atacaacaat gtataaaaca agtattagaa ccaatctgcg aagctaaatt
    11161 tagtgaaaac agctatggtt ttagacctaa taggtcagtt gaaaatgcaa taaaagcaac
    11221 ctataaccgc ctacaaatca gccagctaca ttatgtgatt gaatttgata ttaagggttt
    11281 ctttgacaac gtgaaccatt ccaagctgat aaaacagata tgggctatgg gaatacggga
    11341 caagcacttg atattcattt taaaaaggat attaaaagct ccaatcaaaa tgactaacgg
    11401 tacaataaca tatccagaga aaggaactcc gcaaggggga ataatatccc cactactagc
    11461 aaacattgtt cttaatgagt tagaccattg ggtagaaagc caatggcagg aaaatcctgt
    11521 tacaaaaaac tatgtggttc atatcaataa aagcggtagc ccatgtaaaa gcaacgctta
    11581 caaagaaatg aagaaaacaa agttaaagga aatgtatatg gttcgttatg cagatgattt
    11641 tagagttttc tgccgctaca aggaaagtgc tgaaaaagcc aaaatagcaa tcactcaatg
    11701 gattgaacaa cgattaaaat tggaagtatc acaagaaaaa acaagaattg taaatgttag
    11761 aaaaagatac tccgactttc ttggtttcaa gattaaaatg ataccgagaa gaaagaagtt
    11821 agttgttaaa tcacatataa gtgataaaca attcaaaaac caaaaagaca agctggtatc
    11881 acaggcaaag aaaattgctt ctccctcaaa agggaaaacg gaattagaag aaacaagact
    11941 ttataattca atggtattag gtatgcaaaa ttattatcag attgcaacga atgtaaatct
    12001 ggattgtatg aaactgaata gagctgtaat ggctatcttc actaacagat taggtacaaa
    12061 caaaaggggc agattgagaa aaacaggcag acaacttacg acaaaggagg caacaagata
    12121 tggtaaatct aaaatgcttc ggtatgttgc tggcgagggc gaacctattt tccctgttgg
    12181 atatatccaa cacagaaaac c
//
LOCUS       DAJSVP010000072        12198 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSVP010000072 DAJSVP010000000
VERSION     DAJSVP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.075"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(89..646)
                     /gene="frr"
                     /locus_tag="ON449_002028"
     CDS             complement(89..646)
                     /gene="frr"
                     /locus_tag="ON449_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3934600.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(649..1371)
                     /locus_tag="ON449_002029"
     CDS             complement(649..1371)
                     /locus_tag="ON449_002029"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3934601.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1507..2388)
                     /locus_tag="ON449_002030"
     CDS             complement(1507..2388)
                     /locus_tag="ON449_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3934602.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2487..3269)
                     /gene="rpsB"
                     /locus_tag="ON449_002031"
     CDS             complement(2487..3269)
                     /gene="rpsB"
                     /locus_tag="ON449_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3934603.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3628..4107
                     /locus_tag="ON449_002032"
     CDS             3628..4107
                     /locus_tag="ON449_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3934604.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4324..5415
                     /gene="phnW"
                     /locus_tag="ON449_002033"
     CDS             4324..5415
                     /gene="phnW"
                     /locus_tag="ON449_002033"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3934605.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5408..6193
                     /locus_tag="ON449_002034"
     CDS             5408..6193
                     /locus_tag="ON449_002034"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3934606.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6539..8230)
                     /locus_tag="ON449_002035"
     CDS             complement(6539..8230)
                     /locus_tag="ON449_002035"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3934607.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8652..9599)
                     /gene="arcC"
                     /locus_tag="ON449_002036"
     CDS             complement(8652..9599)
                     /gene="arcC"
                     /locus_tag="ON449_002036"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3934608.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9714..10733)
                     /gene="argF"
                     /locus_tag="ON449_002037"
     CDS             complement(9714..10733)
                     /gene="argF"
                     /locus_tag="ON449_002037"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3934609.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10824..12053)
                     /gene="arcA"
                     /locus_tag="ON449_002038"
     CDS             complement(10824..12053)
                     /gene="arcA"
                     /locus_tag="ON449_002038"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3934610.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3347 a         2536 c         2191 g         4124 t
ORIGIN      
        1 ttttttcttt ttttaatttt taaataaaag aaatataaat aaaaacagag gaagcggaat
       61 gatccgcccc tctgttttta tgattttatt aaacttccaa taattctttt tctttatcag
      121 cagcgatctt atctacttct ttaatgctgt tatctgtcaa tgtttgtacg tctttttcta
      181 atccgcgcaa atcatcttcg gtaatatcac catttttttg ttgtttttta tattcatcga
      241 tcgcatcacg acggatgtta cgaatcgcga tttttgcatt ttctgcttct tttttcacat
      301 ctttagctaa ttcttttctg cgttcttctg tcagttgcgg gataactaag cgaatgacat
      361 ttccatcatt ggttggactg attccgatat cacttgccat aatagctttt tcaacatctt
      421 gaagaatgct cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga atttggattg
      481 atgccatttg gttcaatggt gttggcgcac cgtagtagtt cacagtaata cggtccaaaa
      541 ggctagcgtt cgcacggcct gcgcggattt gcccaagttc acgttgaaga ttttgtgctg
      601 cttttttcat tttttctttt gcttctgtca taattgcatc tgccattttt atttccccct
      661 tacagttgtt ccgatatttt cgcctaagat tgcacggcga atatttccgt gttcatttaa
      721 attgaagaca agcagtggaa tatcattatc catgcttagt gaacttgcag ttgaatccat
      781 gacttgtaat cctttggcaa taacttccag atgagtcagt tcttcaaatt ttactgcatt
      841 tgcatctact ttcggatcag cagaataaac accgtctaca ttgtttttag ccatcaggat
      901 aacatctgcg ccgatttctg ccgctcgtaa tgctgctgtt gtgtctgtcg agaagtacgg
      961 gttgccggta ccgcctgcaa agataacgat ccgtcctttt tctaaatgac gttctgcttt
     1021 acggcgaata tatggctctg cgatttgacg catttcaatc gaagtttgca cgcgtgtcgg
     1081 tacacccacg ttttccaacg tatcctgtaa agctaaggca ttcataactg tagctaacat
     1141 acccatgtaa tcagcttgag cacgttccat gcccatatgt gctccgattt gtccgcgcca
     1201 gatatttccg ccacccacaa cgattgccat ttcaacgccc agctcgtgaa cttctttgat
     1261 ttcttggatg atttctttga ttactggtgg tttgattcca aatcctgcat ccccagctaa
     1321 agcttctcca ctcaatttta agactacacg ctgatatttc ggtgtgacca tgttcattcc
     1381 tccaatattt tctattgcca ttttaccata cttagatgat ttcgacactc taagactggt
     1441 ttttcttgtt ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt ctgttattca
     1501 acagaattat tttttcactt ggctcataac ttcttctaca aagttatctt cgcgtttttc
     1561 gataccttcg ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt ttgatgcaac
     1621 gtatttttca acagtcatat caggatcttt aacgaatggt tggtccacca aagcgatttc
     1681 tgctttgaat ttcttcaagc gtccttctac cattttttca acgatattcg ctggtttgcc
     1741 ttcgttcaat gcttgttctg taagaacagt tttttcatgt tctaattcag cttctggaat
     1801 ttgtgattcg tttacataac gaggattgat agctgctacg tgcatagcta cgtctcttgc
     1861 aactgtttcg tcagttgttc catctagaac agtcaatacg gcaatacgtc cgcccatatg
     1921 caagtatcca ccaaatgcag cattgtcgtc tttttctaca acttcaaagc gacggaagct
     1981 gattttttct ccgataactt gagttgcttc gatcaagtca gattcaattg tacctttatc
     2041 tgttttgatt ttcattgcag cttccatgtc tgctggtttg ttttcagcaa caagttctgc
     2101 aatttctttt actaaatctt ggaacatttc gtttttagaa acaaagtctg tttctgagtt
     2161 tacttcaacg attgctgcag tgttaccttt cacagcaact gaagccaatc cttcagctgc
     2221 aatgcggtca tttttcttcg cagcctttgc cattcctttt tcacgtaaaa gatcaactgc
     2281 tttttccatg tcgccttcta cttcaactaa tgcttttttc gcatccatca ttcctacgcc
     2341 agtcatttcg cgtagttctt ttaccatttt agctgtaacg tctgccatgt ttaactcctc
     2401 ctgtgtgttt ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt gtcgcccagc
     2461 ctttgaaaca gctccatcat tcaaaattat tctgctgaag aattgtcgcc ttcaacaaca
     2521 tctacgattt cttcgattga tgttgctgtg ttttcttctg tgaaatcttc ttcaactact
     2581 tgatcttcac cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt aatcaattta
     2641 acggcacgaa ttgcatcgtc gtttgaaggg attactacgt cgatttcatc tggatcgcag
     2701 tttgtatcaa ccatagctac gattgggata tttaatttgt gtgcttcttg aacagcaata
     2761 cgttctttac gtgggtcaac gatgtacatt acatctggga ttcttggcat atcagcgata
     2821 ccgcctaaga atttttcaag acgttcacgt tctttgttca aaccagcaac ttctttttta
     2881 ggtagaactt caaaagttcc atcttcttcc attgcattga tttgtttcaa acggctgata
     2941 cgtttttgga tagtatccca gtttgtcaat gttccgccta accaacgatg gttaacgaag
     3001 tattgtcctg cacgagttgc ttcttctttg attgcttctt gtgcttgttt ttttgtacct
     3061 acgaataatg ctacgccgcc ttcttcagca acgctcttca tgtaatcata agctgcatct
     3121 actaatttaa ctgttttttg caagtcaatg atgtagattc cgtttctttc tgtgaagata
     3181 tatttcttca tttttggatt ccagcgacga gtttggtgac caaagtgtac gccggcttct
     3241 agtaattgtt tcatagaaat tactgccatg tttgtttcct ccgatttggt tttatttttc
     3301 ctcttcttat cttctacctt gccggcaaac tttttcaagc acctaccgac aatccaataa
     3361 gaatgtggat ttgctgatgc ataaagcacc ctcagctatt atacagacaa aagatccgta
     3421 tcgcaagagc aaatatactt ttctgctcaa cattcaatag tttacctgaa agataaaaag
     3481 aattcaagaa aaacagtaga tttctctttg tttctttatt atcctttttc cataaaataa
     3541 ttttaggtat aaaagacgaa tattattggg caatccttct tttttttgtt atgatacatg
     3601 tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt gctgattaaa
     3661 caagtcattg aagaattccc gatccgtacg caagaagaac tattaaacaa acttgggcat
     3721 tatggcgtta ccgccacgca ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg
     3781 aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct
     3841 gagactagga ctgaggaaga ggatcggctg gttcaaatga ttgaagacat tgtattgaaa
     3901 gtcgaacgag tccacttttt gacgatcgtc aataccttgc cagacaatgc tcatttattt
     3961 gcatctgttt tagatgagat caaacctccc tctattgtca gtacaattgc tggattcgat
     4021 acgatcatgg tcatctcgga aaatgaggag gacgcaaaac gagtagaaga atttcttcac
     4081 catccaactg aagtatcact tttttaatca gagcacgtat tcctaatatg gaatgcgtgt
     4141 ttttttattt tcaatatttt ccttattaaa taaaccttta cactctttac aaaaatacat
     4201 cattttttat tcaatttttt caacaccttg attgaatcac aataatcatc atttatattg
     4261 gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat
     4321 catatgtata aattattaac tcctggacca ttgacgactt ctgaaacagt gaaaaatgct
     4381 atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga
     4441 aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa
     4501 ggtagcggct cctttggggt agaatcggta ttaagttctg tgatcaggcc agaagatact
     4561 gtactgattt gttcaaacgg cgcatatgga caacgaatga tcaacatgtg cgaatgtctg
     4621 aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt
     4681 acacagctga ttgaagagaa tccctctatc tccgcactag caatggtcca tagcgagact
     4741 acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga aaaagatctt
     4801 ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgattcgtgt agccgaacta
     4861 gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct
     4921 tttgtacttg ctaataaagc ggaactagaa aagcgaaaaa atgtgagtcg aagtttgtca
     4981 cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact
     5041 tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga
     5101 ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtactacg acaaaaaatg
     5161 acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt
     5221 ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaac
     5281 ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt
     5341 ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag
     5401 aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg
     5461 tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga
     5521 caaagaaatc cgcgaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc
     5581 cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga
     5641 ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc
     5701 taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat
     5761 tggttccacg actggattta ctgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa
     5821 agaagggtat tcaccagact tcatggcaac ttctgaagac gtcgaagggt atggtcgacc
     5881 gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt
     5941 aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac
     6001 agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt
     6061 agagatgggg aaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagctgg
     6121 cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacaa
     6181 gaatccagaa tgacttttat acaaaatcag aggtcgtgaa aaagctgtac tgcatcaagt
     6241 aataagaaca gccaaacaaa aatagctcct catatttttc gtttggttag gcttattacc
     6301 gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa agggagctgt gacacgactt
     6361 ttgtcacagt ccccttattt acaaacaaac agtgtttaag agacaacctc tccactaaac
     6421 ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat
     6481 tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt
     6541 acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca
     6601 ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat
     6661 gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg
     6721 gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca
     6781 cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg
     6841 ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc
     6901 cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca
     6961 aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca
     7021 aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt
     7081 cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca
     7141 aaccaaatgg aatatggcgc atatcgtcag accagtcaaa gcccatttcc ttcaagacag
     7201 ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga
     7261 agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg
     7321 caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct
     7381 ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca
     7441 tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat
     7501 agattttatt gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct
     7561 cttcatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt
     7621 ctgtttcagc tacttcatgg aattggacat ataaacgaag caattcattg attggttctg
     7681 ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg
     7741 ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga
     7801 aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa
     7861 tattaggaga agacatgtcg attggtactg ttccttgatt cccgatattg ctatccccat
     7921 aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt tctttattca
     7981 tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt
     8041 tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa
     8101 aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgttcga
     8161 cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt
     8221 tattattcat ctttattcct cctaaagtgc tcccttgatt gttaaacaaa aaaaatctct
     8281 agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa
     8341 ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag
     8401 aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg
     8461 ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc
     8521 ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt ctgataaaaa aacaatccag
     8581 atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa
     8641 gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg
     8701 atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata
     8761 gctgcctcta ctttcggaag catgcttcct ggagcaaatt gcttttcatc aatgtattgt
     8821 ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca
     8881 tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat
     8941 ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt
     9001 tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc
     9061 gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc
     9121 catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg
     9181 gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca
     9241 ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc
     9301 ttcagtactt cgcccatggc attttgtaac cagtaaccga tagatccttc tgtcattgca
     9361 acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc
     9421 aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg
     9481 ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt
     9541 gcatccgtag acaagattgc attaccccct aaagcgacaa ctacttttcg tttggccatt
     9601 tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt
     9661 gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg
     9721 cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga
     9781 ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc
     9841 atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg
     9901 aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt
     9961 tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca
    10021 cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca
    10081 tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg
    10141 atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt
    10201 gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca
    10261 gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt
    10321 gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt
    10381 tcctctacca ttttttgact gaatccacgg aattcgattc catcaaacat acgacctagt
    10441 actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga
    10501 tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta
    10561 gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca
    10621 cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt
    10681 gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac
    10741 attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag
    10801 taagaccgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg
    10861 cagcgtggac caccacggcc tcgtgacaat tcacttgaat tgatttctaa cactttgatg
    10921 ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct
    10981 ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca
    11041 tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt
    11101 tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag
    11161 acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct
    11221 accattgtaa atacggtatc caaatgcatc attgcacggt tgtttggaat tttgattgct
    11281 aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct
    11341 ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg
    11401 tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt
    11461 gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct
    11521 tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta
    11581 aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatcg
    11641 tatagactgc tagaacgtac ttcaatatct tttgtacgaa ccccagccat gattttatct
    11701 accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc
    11761 gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg
    11821 tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat
    11881 gtctttgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac
    11941 aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccttttcagt
    12001 aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt
    12061 tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat
    12121 tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaac
    12181 gtttttctat ttttaagc
//
LOCUS       DAJSVP010000073        12040 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSVP010000073 DAJSVP010000000
VERSION     DAJSVP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.076"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(64..2148)
                     /gene="cadA"
                     /locus_tag="ON449_002039"
     CDS             complement(64..2148)
                     /gene="cadA"
                     /locus_tag="ON449_002039"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3934611.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(2672..3031)
                     /locus_tag="ON449_002040"
     CDS             complement(2672..3031)
                     /locus_tag="ON449_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3934612.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3061..3402)
                     /locus_tag="ON449_002041"
     CDS             complement(3061..3402)
                     /locus_tag="ON449_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934613.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3399..4073)
                     /locus_tag="ON449_002042"
     CDS             complement(3399..4073)
                     /locus_tag="ON449_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3934614.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(4869..6167)
                     /gene="asnS"
                     /locus_tag="ON449_002043"
     CDS             complement(4869..6167)
                     /gene="asnS"
                     /locus_tag="ON449_002043"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3934615.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6207..7397)
                     /locus_tag="ON449_002044"
     CDS             complement(6207..7397)
                     /locus_tag="ON449_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3934616.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7418..7945)
                     /locus_tag="ON449_002045"
     CDS             complement(7418..7945)
                     /locus_tag="ON449_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3934617.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(7992..10781)
                     /locus_tag="ON449_002046"
     CDS             complement(7992..10781)
                     /locus_tag="ON449_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3934618.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(10928..11128)
                     /locus_tag="ON449_002047"
     CDS             complement(10928..11128)
                     /locus_tag="ON449_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3934619.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11580..11900)
                     /locus_tag="ON449_002048"
     CDS             complement(11580..11900)
                     /locus_tag="ON449_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934620.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         3327 a         2594 c         2108 g         4011 t
ORIGIN      
        1 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttcat
       61 tgatcaacgt ttatttttta catgcaagct tcgcatggca ttcaaaacag ctaatactgt
      121 caccccaaca tctgcaaata ctgcatctcc cattgatgca aaaccaaaag cacctaagga
      181 aagtaccaaa atttttatcc caatagcaaa gacaatgttc tgtttgacga ttttcaatgt
      241 tttctttgct aaatggattg cttctgcaat cttcgatggt tgatcattca tgatgactac
      301 atctgctgct tcaattgcag catcgcttcc caatccaccc attgcgattc ctacatctgc
      361 tcttgctagc accggtgcat cgttgattcc atctccgaca aatgcggtct ttttattgtt
      421 actttgtaag atttcctcta accgctgcac tttatcttct ggaagcaatt cgctatatat
      481 cttgtccacg cctacttgct tcccgatatg atcagcaatt tttttcgagt cgcccgtcaa
      541 catgactgtg tttttcacac cagcctgttt taattgaacc aatgcgtcta cggcatcttc
      601 tttcaaggta tctgcaataa caagataacc actgtaactt tgatcgatcg ctagataaag
      661 caatgtaccg atttcttgac ttgaaggaaa agagatttca aatctttcca tcaatttgtg
      721 attacctact aatacttgtt tgccatcaac ttcagctaag atcccttcac ctgcaaattc
      781 ctggatatta gctgttggta aaagcggacc tgcgtagcct tctaaaacag attgagcaat
      841 cggatgtgta gaaaactgtt ctgcacttgc aacatattgc ataaatttct ttggatcaat
      901 tcctgttgga tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc cagttttatc
      961 gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt ttactaatac
     1021 accgatttta cttgctcctc cgataccgcc aaagaaacta agtggcacgg aaatcactaa
     1081 tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt cagaaaacgc
     1141 cgctctagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc ctacaacgac
     1201 tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt ttttactgct
     1261 ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag atttttctac
     1321 ttgaactaaa aatggtttat ttttattgat aaatccgctt aatactgttt caccaacata
     1381 tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg attctccttc
     1441 gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa actgaccgat
     1501 cagcacgtct tcagggttca cggtcatcaa tccttccgcc gtctgcacgt gagctgtttc
     1561 tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg ccaatccttg
     1621 gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat attcaccgat
     1681 caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga tttttccttt
     1741 gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat aaatcaataa
     1801 ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag cgatacctgc
     1861 aagggaaaat aaaatccgga acagcgtatt tctctttttt tcatttccat gttcagatac
     1921 tttttcctta tctatttctg ttactttgac atctggttct aattccttca ctactttttt
     1981 tgtttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga
     2041 agtgaagttt accgttgctt ccttgactcc attgattttc tgaactcctt tttcgatttt
     2101 cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt taatgacctc
     2161 ctcgcctcaa catatgaata tctcttcata actaattata aatgaatggt tattcatatg
     2221 tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct tccatacaga
     2281 tgttttggct tattctttct ttttctcttc gatcattgcc actgctctta tccaacccgc
     2341 acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg ctggcaattt
     2401 gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg caaaatgtcc
     2461 ttcccagata tacgaagctc cactacgctt tcaaaaatat agaaagagtt ctttaatgaa
     2521 aattatttca actttccata aacagtaacc ccatataaat aaaagacgta aaagtcacat
     2581 agaagaactt atacaggaaa acgcatagag tcaggtgaaa tacatgtatc ccacctggct
     2641 ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg aatggcatca
     2701 acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg atcgtgatac
     2761 atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa acgatcagcc
     2821 agatgatatt aacgatcaat gacatgccag actggtgata aattacttct ttgccaaaag
     2881 gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata cctatgatcg
     2941 tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt ccgccgaata
     3001 taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta ctgtttttga
     3061 ttaaaactgg gcaaaaattg attttattct tcttttttta gcttccagca tcaagtattc
     3121 tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt gttggattag
     3181 ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg cttgcgcaaa
     3241 tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct tatcatttag
     3301 gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca gctgattgat
     3361 ttgaggtaaa tcttctttta ctgcccacct tatttccatc atttttcact tttctttcgc
     3421 tcatttttat ctccactaaa aaaatcgact gcgacaattg catctatcca gctttgtgca
     3481 atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata tttcgaaaaa
     3541 ccataagcaa tccctaaagc attgaatata ccatctaaag gaaccagcaa tgcgccatat
     3601 tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg ccagccatga
     3661 cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca gatttccgct
     3721 gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc agatttctca
     3781 gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc gatcaaaatc
     3841 gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg cagatctgca
     3901 attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg atattgggag
     3961 attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc ttctgctcgg
     4021 tatcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt catttgtcaa
     4081 aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata agccctatca
     4141 agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagttcctt caaacaattg
     4201 aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg tgccggattc
     4261 cccttgattc cttcgttgtt tggtccagag agatcgatca cacccaagct tccgtcaatc
     4321 agaccactga tgacgccatc gaaacatata tttataggct catctaagac ttctagtaaa
     4381 tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata actttatctg
     4441 gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc tgaaaatacg
     4501 gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga tgctgcttta
     4561 tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc tttgtaaatg
     4621 tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt tcttttgctt
     4681 tcttctcttg tctgctggaa acttccatta tcccgtattt aaaaaaaaag aactgcgaga
     4741 gttcgcagtt ctttttttta aataaacgtt gctctccggc cacaagtcac aatagtcatc
     4801 aaacatgaaa aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc
     4861 acaacctctt atggataaat tcggttcaac aaacgtggga atggaatagc ttcacgtaca
     4921 tggtcgatac cagcaatcca agtgactgtt cgttcaagac ctagaccaaa accagagtgc
     4981 ggaacagaac cgtatttacg tagatcaaga taccatgaat attctttttc atccaaaccg
     5041 tgattacgga tttgttctag caagaaatca tagtccgttg cccgttcgct tccgccaata
     5101 atttcaccgt aaccttctgg tgcgatcata tctgcacaaa ttaccacgtc ttctcttgtt
     5161 ggatgtggtt tcatgtagaa tggcttgatg gcttttggat agtttaggat aaagacaggc
     5221 tgttcgaatg aattcgcaat gaacgtttca tgaggtgaac caaagtcttc gccccattcg
     5281 atgtcatcaa atccattttt cttcagtaat tcgattgcat catcgtaagt aatacgaggg
     5341 aatggcaatt ttgtataagt ttttaagact tctttgtctc gtcctagtac atctaatgcg
     5401 tattcgcaat gatctaaaac attttgtact aagaatgcta catattgttc ctgtacttct
     5461 aaactttctt cttgatgagt gaaagccatt tctggttcga tcatccagaa ttcgatcaaa
     5521 tgacgtcgtg tttttgattt ttcagcacgg aaagtcggac cgaatgaaaa gacttttccg
     5581 aaagccatag cagcagcttc catgtataat tggccacttt gagataaata agctttttga
     5641 tcaaagtaat ttgtttcgaa tagatccgta gtgccttctg gtgcagaacc agtcaagatc
     5701 ggcggatcaa ttttgacaaa atgatgttga ttaaagaatt catacgttgc acgaatgatt
     5761 tcatttctga tctgcataat cgcatgttgt cttgaagaac gcagccataa atgacgatga
     5821 tccatcaaga agtctgttcc gtgttctttt ggtgtgatcg gataatcgtg gctctcgctt
     5881 accacttcca catttgtaat tccgatttca taaccaaatt ttgaacgact gtcttcacgg
     5941 atttctccag ttactaacac ggatgtttct tggtgtagat ttttagctgt ttggaaaact
     6001 tcttccgaca cttcgctttt tacaactaca ccttggaaaa aggctgttcc gtctcttaat
     6061 tgtaaaaatg tgatttttcc gcttgatcgc ttatttgcga cccaagcgcc gattgttact
     6121 gtttctccca catggttttt cgaatcgatg atttgaattt gttccacgta tgactctcct
     6181 tttatattcc ttgaatttct tcaggattaa ttttgacttt tactctcaat aaattgcttg
     6241 attctgcgta ccgcttcttc taacgtagcg cgatcagtag cataactcat gcggacattt
     6301 tctggtgccc caaagccttc gccagtcaca agcgctaccc ctgtttcttc taaaagatct
     6361 tctacccatt gtgtgacatt ttcatattta cacatgcgta gcgtttcttt tacattagga
     6421 aaaagataga aagctccttg cggcttttca agagaaacac ctggcagttc tgccacaagc
     6481 ggatataacg tattcaggcg ttcttcaaag gcgttccgca tttcttctac tgtatcttgc
     6541 tcacctttca atgcttcaac agctgcatat tgactgactg ctgtcggatt actggttgac
     6601 tgggaagcaa cagcaatcat tccattgatg atctcagaat tccccaccgc ataaccaatg
     6661 cgccagcctg tcatcgcata tgttttagac acgccattga tgatgatggt ctgatttttg
     6721 attgcttctg aaatcgttgc aattggagta aactcattgc cattataaac taaacgacca
     6781 taaatatcat cagcaactat caaaatatca ttttttactg cccattcacc gattgcctga
     6841 agttcattct tgctataaat cattccagtc ggattcgatg gagaattgat gattacggct
     6901 tttgtttttt ctgaacgtgc ttgttctagc tgttcaacag taactttcca gctattggat
     6961 tcttcccctt tcacaaagac aggaagtcct tctgccagtt ttacttgctc accgtaactg
     7021 acccagtaag gcacaggaat gatgacttca tcttgcgggt caaggatcgt ttggaaaagg
     7081 gtatacaaag caaatttcgc tccgtctgtt acgactgttt gtgaaggttc atagacaagt
     7141 ccatagtttt tcttcagata atcaacgatt gcctgtcgaa gctctgggat tcctgctgtt
     7201 ggtgtataat agctagcttt cccattgcgg attgcttcga ttgctgcttc ttggatgttt
     7261 tctggtgtcg caaaatccgg ttctcccaca gtcaaactca gcacatctct gccttttgct
     7321 tttaatgcct tggcctttgc cgcagctgcc aaagtgacag acggttctaa tcgttttgtt
     7381 ctttttgata actccatccc attcattcct ttacctttta aattcccttg attgtcttga
     7441 cttcatctcc agattcaaag ccaatcaggt aataattcag tttcccattt gtttcttttc
     7501 ctactacttc ccacacaggc tgatcatcat acatccctaa agcagctttt tcgatttgga
     7561 tttcaggatg ggccgcagca attgtctgtt ttgcttcgtt ttcagtcagt ccctttgttt
     7621 gatccaatac ttttactttt tcaccagatt taggaatgat aacggcgatt tcttttcctt
     7681 tatcatttgt acctgtcagt gaaaagtatg tcttctttcg agtaaaccaa taaaaatgat
     7741 cggcttcctc aagatttgca tatttctttg cgatttctac tgcttctttc ttcgcctgca
     7801 ccgccggacg actggaacgc aagtaaaaaa tcgagctgat cacgatgacc gctaataaaa
     7861 atacgatcaa gccaagtaac acgcgattaa tcgttttttc ctttgtctgt tttttcttca
     7921 agggtcgtcg ctcctttatc ttcattcctt ttcattatag cagattttaa ttatgaaaaa
     7981 agacagctcc tctaacttcc ttcttcattt ttctttaaga attcatggat gtcctgatag
     8041 atttcctcca tagctagctc tttgatcggt acttttttcg gtaatgcatg gatgatccgt
     8101 gcaccatatt ttgcagtgac tagtcttcga tccaaaataa gcaatacgcc acgatctgtc
     8161 tccgtcctta tcaagcgacc taatgcttgg cgtaacttca aagctgcttt aggtacggat
     8221 tcctggtaaa aaggattgat cccctctgat tctaaataag cgttcctagc tttgaccatc
     8281 gggcgaagcg gattatcgaa gggtaaacgt gtgactacaa cgatctgcaa agcatcccca
     8341 ggcaaatcta caccttccca aaagctgtca gcgccaaaca atacgctttt ttctgacaaa
     8401 atgaagcgtt tcaaaagctt ctcccggctt ccgccaaacc cttgagctag tatttctcgt
     8461 cctttttcta gcatagacag atgcattttc tgatagacct tctgcaaaac atcatgagaa
     8521 gtgaataaca caagtattgg ccgatcttct tgttcgatca gacctgtaag tgctgtctct
     8581 aaataatcga tatatgcttg attgctgact tgatggacgg caggtgcctc ttttggcaca
     8641 tagatccttg cctgtttctc ataatcataa ggagaggcaa tcactttaag aggtgctgct
     8701 tcaatgccaa gccgcttaga gagatagtgt cgattagggc cgatcttcag cgttcctcca
     8761 atataaagaa tacgatcata tcgaggatac caaacagtcg acggcaacat agccgcttga
     8821 aaatcatgga gatgaaaaat catctgctgc gctgtgttcc caggaacgaa ccaatggatc
     8881 aaatgatctc gccagtcttc cagccattga tccatcagca agtactgctt gtctaattga
     8941 tcaaataagc tatataaact tgctaaatcg atccgcatct ggtttgtcca gctttcttgg
     9001 ataatctcaa aagtcagacg catttgtgac tgtagctgct ggatctcctg ataaaaaagc
     9061 tgcaggtgat caatggctgt cccagattcc agtggcagct gatccaacat ctcttttgtc
     9121 atcatgactt cttctggata ctgtccgtta ggttgcttgc cgctcactaa actcattaat
     9181 ccttctgcca tacaaatctg tgcttcgcat agcgcagaaa gttcttctcg ataaatcgat
     9241 aaccagcgta aggcttcatg ttctttcggt aaatagcttt gtattcggtc aaacagctga
     9301 tttggttctt ctaattgctg gatttgttta tggaaatagt taatacttaa tcgtttgtca
     9361 tttacttttt cagcgatttc cggcaaatga tgtacttcat cgatcaacaa aaatggacta
     9421 gctggaagca atggttcttt tcgctgtgtt tcttgtatta aaaacgcatg attgacaatc
     9481 aaaaaattac tttgcctgat tttcttttgc aagaaacgaa caaagtcttg ttcgtaaaat
     9541 gactgatttc ttgctaaaaa tccaatacct ctgtgcgcga tatctgcaaa gaatgaatgg
     9601 ttcaaatttg ttaaatgtaa ttcatccaaa tcaccggtct tcgtttgcgt cagccaaact
     9661 aagaccgcca tttgatacaa agcatattgt ttttgttctg gcggttccac aagtgtgcct
     9721 ttaaaacgct gtaaatcaat atagtggcga tagcttttga taattacagg ttgtatgggc
     9781 tgatctagca attgattcag caatggaatg tctttttcta tgatctgctg ctgcaaaact
     9841 agcgaaacgg tactgatgac agcgggtttt tcaggtgtag ccaaataact caatggcagc
     9901 agataaccga ttgttttacc cattcctgta gctgcttcga tcatcaaatc ttttgattca
     9961 tcttttgtga aatggtcgta gaccgcattc atcagtctgt tctgttcttt tcgatacatc
    10021 agtgttttac caaacatttt ttcttttgct tgtttttttc ttggatatgt ccgctcgcca
    10081 taatggacgg atgtgaatag ttccacttct tttttctgca aagctagccc atcgacgatt
    10141 tctaatgagg gatcaagtgg ctctggtttt tctttcattt cttcaatcac atgataaatg
    10201 aaacgactcg tatccatacc tgtgtgtcta cttaatcgcg caatctgctc taacgtgatg
    10261 attggcaatt ttctcatccg ttcttcgatc agcaaaagaa gttgacccgt cacttcggca
    10321 tcactatctg cctgatgagg attctcatga ataaatccaa ggctttctga caaatcagct
    10381 aagcgaaagc ttggttccgt cggcaagaaa atttgtgcaa gttctactgt atcgatgcct
    10441 ggtatcttca aactaggaag tccgcatcgc atgagttcat ggttcaaaaa attataatca
    10501 aaataaatat tatgcgcgac aaatatggtg tctgccaaaa gattgtaaat cgtttgggcg
    10561 acatcttcga aataaggcgc tttttggacg cgttgattcg tgatccctgt taaatgctgg
    10621 atttgttttg aaatcttccg ccctggatga atatcgatcg aaaaacgtga agtgatccga
    10681 cctgattcaa tcatgacgca gccaaattgg atgatccgat cttcttttgg attcgtccca
    10741 gtcgtttcga tatcaacgac tgcataagtt cttggtttca aaatatcttc ctcttctcct
    10801 gcctctaatg ttcttttgaa attatactac aaagtgcaga aaaatactct catcgctttt
    10861 taaagaaaat agacaaaaac gcccagacac gcaaatgtct gggcgtttat tttttagtat
    10921 aggttaatta agctttagta acgtttgttg cttgaggtcc gcgttggcct tcttcaacat
    10981 cgaaagttac tgcttggcct tcttctaaag ttttgaatcc gtcgccttgg atagctgaga
    11041 aatgaacgaa tacatcgttt ccgttttctg cagtgatgaa tccaaatcct ttttctgagt
    11101 taaaccattt tactgtacct gtttccataa taaaaatcct ccttgtgtat caacacatat
    11161 tttgcaatta cttgaatggt gtatatctgg aagatgttta tttgaaacaa gctaccacag
    11221 attaccgaac aaaaactact cctatagtat atcataccgt ttacaaaaaa gctagagttt
    11281 ttcgactttt tttattaaaa aaagttttct cagcgattac ataaagtgaa aaaccgaact
    11341 ttttttaaaa taaacgacat attgttcagt tggtgttttt ttgctctgat gttcttcttt
    11401 aaactaaaaa aattattctt tctgactact tatcggttct gtttcaacgg tcgaggctgg
    11461 ggaagattct ttagtaggtc tgatgttctc atggtatttc tgatagccta tgacagttac
    11521 cagaagaaca aggcaaacca atacgatcaa cgagattagt ttttcttttt tgctcatatc
    11581 tatacctcat aaataacagg atgatgtcca tcatatttct gagtatgagt aaaagcaaaa
    11641 gctgcagctg tagaatgtaa gaaatgccag caactttcat aatcacaggt cggacagtac
    11701 caaaagtaac caaatcttgg ctgcaaagac cgttcttctg tttcaggaat cgcagaatca
    11761 agatctgttg tctgtacata aacatttttg tacagacgtg tgcctgtaat aggagaccgc
    11821 tgtgtttctt cagcaaaaac tggctgactt gttaaactta gggatagaaa cagcaaacac
    11881 cataacagtc tttttttcat aaagacctcc ttatcgataa gtttttccca attaaaatca
    11941 tatctcaaga acgcccgtta ttcacatttt gttcataaat gatgtttttt gtatcataat
    12001 cgtcaaaaag aggtcgtgac cacgtcacaa cctctttttt
//
LOCUS       DAJSVP010000074        11819 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSVP010000074 DAJSVP010000000
VERSION     DAJSVP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.077"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(114..1577)
                     /locus_tag="ON449_002049"
     CDS             complement(114..1577)
                     /locus_tag="ON449_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3934621.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1641..4658)
                     /locus_tag="ON449_002050"
     CDS             complement(1641..4658)
                     /locus_tag="ON449_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3934622.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4809..5648)
                     /locus_tag="ON449_002051"
     CDS             complement(4809..5648)
                     /locus_tag="ON449_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3934623.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5793..6038
                     /locus_tag="ON449_002052"
     CDS             5793..6038
                     /locus_tag="ON449_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934624.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6101..6523
                     /locus_tag="ON449_002053"
     CDS             6101..6523
                     /locus_tag="ON449_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934625.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6617..6934)
                     /locus_tag="ON449_002054"
     CDS             complement(6617..6934)
                     /locus_tag="ON449_002054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934626.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6961..7167)
                     /locus_tag="ON449_002055"
     CDS             complement(6961..7167)
                     /locus_tag="ON449_002055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934627.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7148..7330)
                     /locus_tag="ON449_002056"
     CDS             complement(7148..7330)
                     /locus_tag="ON449_002056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934628.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7344..7694)
                     /locus_tag="ON449_002057"
     CDS             complement(7344..7694)
                     /locus_tag="ON449_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934629.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7720..8022)
                     /locus_tag="ON449_002058"
     CDS             complement(7720..8022)
                     /locus_tag="ON449_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934630.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8025..8258)
                     /locus_tag="ON449_002059"
     CDS             complement(8025..8258)
                     /locus_tag="ON449_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934631.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9244..9566)
                     /locus_tag="ON449_002060"
                     /pseudo
     CDS             complement(9244..9566)
                     /locus_tag="ON449_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9689..10804)
                     /locus_tag="ON449_002061"
     CDS             complement(9689..10804)
                     /locus_tag="ON449_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3934632.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10810..11208)
                     /locus_tag="ON449_002062"
     CDS             complement(10810..11208)
                     /locus_tag="ON449_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3934633.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3141 a         2414 c         1814 g         4450 t
ORIGIN      
        1 gatagatacg agatttcaaa ttaaaaaacg aaaatgagag atacaaatat atcaaatatt
       61 taaaaagtaa ccacattttc tataccactc cttatcagaa acagccttat tctttagaat
      121 aagactgttt ctgggttgat taatttgtct tttgttaaac taccatctaa ggcaagttct
      181 aaatgtaaat gtggtcctgt actatttcct gttgaaccca cataaccaat aatttgtcct
      241 tgttcaactg tttgtcctac gttcacgcaa tattcctgtt ggtgcgcata aagagccaca
      301 cttccgtctt catgctcaat tgtgacataa tttcctgaca aagtatgata cgaagcttct
      361 atgaccgttc ctgccttgct cgcatagatt ggttctcctt gattggctgc catgtctaat
      421 cctctatgaa attcttctcc tcgccaaccg aaggaactag aaattgaata attttttaga
      481 ggtaccatat agccttcttt gcttagttgt ggaccagcta cttctttatc atatttgatt
      541 aagtcatagg tttcgattaa cccatttaat tttttatcgt agctagtgtc tgttgcgtat
      601 cttcctgtta aaaatttagt cgcttcctga taggtttttg ccttactttt ccaaacacca
      661 tcataaaaag aggtatttcc aactaagcct tcttttaaca gtttggcata gtcttttaag
      721 ctttcttcat agctctcata ttgccgaaaa gttgcttggg tcgtataaag tgtcccattg
      781 cctaaatctt cttgtgtcgc aaaagagacg ccctttccgt tatgtgtgcc tttgattcca
      841 aatagattgt agtttggtgc ctgagccaat tgactttgtc cgcttgcgga ttctaaaatg
      901 gcttgcgcga tcataacaga agcgtataaa tcattttctt gtccaatttt acgagcagat
      961 tcaccgattt tacggataaa cgtttctaca gacgcgtctt tttcaaaatg aatagtccct
     1021 tcgttatctt gtgtgctgga agggactaat tcttgtatcg gtgcgcttgg tgcttggtgg
     1081 tagttttttg tctcttctga accgattgat tgtgcctgcg gtacgtcatt cataggtaca
     1141 gtcacactct gttcaggtgc cttttgtggg cgttccgttt ttgttggcgg ttccgcttct
     1201 gtttcggtac ttggtttttg aggagtctgt gagctttcat cagaaggttg ctcatttggt
     1261 tcttctgacg gttcctcctc aggttgttga ttgtcttctg gtttgtctgg ttgattcggt
     1321 gtttctggtt gttcttcaga acctgttggt ggagcgttca tttctggatt atctggtgta
     1381 tctattgtat cttcagtcgg tgtttctggt tgattttctt ctccagtgga atttgttgtc
     1441 gtttcttcag tgatcgttgc agattgcgaa tttgtcagtt gattgttgcc ttgttcatta
     1501 gccaaaaccg ctggcgtata cccaaatact tgacctacta ataacaaagc agataaacat
     1561 ttactcgttt ttttcacaag attgcctcca ttcttcaatc aatcatctag agacagctga
     1621 ttgctgtctc tagtcatact ttaattgctt tgtttcattt tacgacgaac cagaacagcc
     1681 cccacaccaa caaaaagaaa cagtcccaat gcaattaaca catcgttcac tgtttctcct
     1741 gtttttggta agctttgttt tggtgtgctc gtcggcgttt gtttgacagg ggttgtcggc
     1801 gtttcttttt taatgccttt tgctgtcaat gtttgttttt cattcttcaa atcaaagtga
     1861 cgtgcttctt cctctttatg gtctttatcc ttatagacaa tatgagttgc gaccaatttc
     1921 tccccgtctt ttaacgtgcc tgctttgtag tcaaaggata ctttaaactc ttcagacgct
     1981 ttagaaactt tacgtgcagt ttctaccgta ccaacgactg tatcttttcc atttttatca
     2041 atcttatgga attgagtcac ccagtatttg gtttccccta ccgtagattc tggaaattct
     2101 tgcgatacat gatcgatcaa ctcttggtct ttgtctggat caaatgtttg gtcgccattc
     2161 actgtaataa agcgtgtttc gatttttggt tctttcgcag tcactgtttg atctttattg
     2221 gttaaatcaa aatgacgagc gaattcttct tcctcttctt tgtcgttata gacaatgtgc
     2281 gttgcgacaa tcgtgtcacc gtctgaaacc atgtctgctt tgtagttgaa cgtcacatca
     2341 aatgtttctt tttcatcggt cacttttcgt gtttctttta ccgtatctaa tacagtgact
     2401 tttccatcgg cagtaatttt atggaattgc gtcacccaat atttctctgt cccaaccgca
     2461 gatttaggga atacttggga cactttatcc actaattctt ggtctttttt cgggttgaaa
     2521 tcttttccac ctgtcgttgt cacaaacaaa gtagaaattt ctggttggtc ttcttctgga
     2581 accacgattt cttcgttttt cacagtaatt ggttgtactt ctccgtcttt gaccgttact
     2641 aaaatcgttt ctgtttgtcc tgacgcttgc ccataatgct cagggaacac aatttccgta
     2701 aatttatacg taccgtctgg taattttagt ttttctggtt cttcttttgt ttcatacaca
     2761 aaggacacat ttttattatg tgcagtcata ccttcaatca ggaatttcgc tcctggtaat
     2821 tgtagatgat tgattgaatc gactttattg aagtctactt cacccgtttt taacaagttg
     2881 tcaatcacat ctaattcaaa cgtaagcaca ggcgtcgtgt tatcgccata ggccacagag
     2941 aacgtggttt gttcttcaga aggttgacgg tagttgtctg gcacattttt ttcaatcaat
     3001 tggtatttgt tttgatcgcc attgtagaat tttttgtttg ccttccagtg tccgtctttg
     3061 tcagttttta ctgaatccaa gacttctcct tttttcacaa ggactgtttc tttatctggc
     3121 gcagtaatat ctgttttggc gataatatca aattcgatat tttctgcagg aacggtatca
     3181 taaacaggtt tgccttgatt ccaggattct tccgcattat agacttcttt gactttcttt
     3241 ccttctggaa tgaactgttg taagtcattt tttgcagtta acgtcacttc tttgtttggt
     3301 tcaatcgtaa tcgtatgagt ttgtttgtct agaatatacc cagctggtgc tttgacttct
     3361 ttgacatcca atttattttg aacatatttt ccgtcttttt catagaatgc agggatttct
     3421 tttaaggcga tttccccttt ggcatccgtt gtatattgtt ttgtttctcc cgtatcatga
     3481 cgtttgattt catacgtcac atttggaact tttttccctg tgacagcgtc tagtttggtt
     3541 agtttgaagt cgccattttt tagaacttta atagttagtc cagctctatt aggatctggc
     3601 acataaagct ttcctagatt ttgagcaaca ggatctacat aggcaaaact tgaacctacc
     3661 cctctagaat ttggatccag tgaagaaaca gaaattcgtc cgtcatttga gttttgattt
     3721 gcttttataa acaaactatt tcctgatttt cttacttgaa cgttagctgt gttttcaaca
     3781 accgacccat cccattgaga aaacacacct tttgtatcct taacttctac ttcttcccct
     3841 actttaaccg tcactgtttg gctatcaaaa gaaggatctt caagtaaatt gtttattttt
     3901 ctatctacag tctgtttgaa agcttcataa ttagattgtc caatagttcc ataattaaca
     3961 aatgaaccac ctaaaacttc ccaaatatat gtttgtgcta atacatagtt atccattgaa
     4021 ccactagaat agtagccaaa ataaccaatt aaagaaatct tttttctaat agcacttgtc
     4081 gtaattaaat tttcgagtgg tgtcacatca tattgaatac ccgcactcgt gattgcgcct
     4141 tcttgaatgc aaaaaacatc ttctccgtca atttgccagc gaaccatgta atcatgagta
     4201 tacattgttc cttcaggagt aattcgagtc atattcacat ctttacgacc attttcagtc
     4261 aatgttagat tggtagcacg tgtctgtgca ttcttttcca agattgcttg ccattcttca
     4321 gattcttgcg ccaatcgttc ttgttcagaa aaagaagtcg ctttttgaat atcttcaatg
     4381 gttataaagt atttctcata accttcaggg acctcttgag tcattacatt gggcggagaa
     4441 acatcttctg tcgtgtcctg cgtagttgtc tcttctgttg tttcacttgt ggtttcagta
     4501 ctgatcgctt cttcactact ttttgattct tcagtcttca ttacttcttc agtagaattt
     4561 gattcattta tcgtttctgc aaatgccgtt acagatccta acatgggatt gagaaatgta
     4621 acgaaaattg ccaaccaagt tgccattctt ttaaacatat ttttttcgtt cctttcattt
     4681 tatttttcag tcttctaaag taatttctta tggacttcga caccgaaaca tttttatcct
     4741 cctcaaaaca aaaaaagact agaacttata attctagtct tcatcaacca cttacttttc
     4801 ttttattttt agtagaaatc tacagtgtgt ttttgtgacc cgtcactgta aataataaca
     4861 tccaccataa aacctttgtt gtaccactgc gaatttggat cgctaacctc tgcattagcc
     4921 caatcatagg cttcatctac agtgttaaat acctttccac tattaccaat ctctccaact
     4981 acaattgttg gctcttcagg tgtcgttgtg ccaccatttc cgccttgatc gcctccacca
     5041 ggattgcttg gttcttcagg cgttgttgtt cctccacctg tgcttgggtt agaaggagtt
     5101 gtttctcctc cagtatttcc accattacct ggttgtgtag gtgtttgagt agtgccacca
     5161 ttgttttctg gtgtacttgg cgttgttgta ccaccagttg ttcctgtttg atctccagct
     5221 ggtgttgttg gtgtcgttgt atttggtgtc gtttgtgtat ttgaagtcgt tccttgtcct
     5281 actggttgat tcggatcaac tttgtctttc tcttgaatca ctgtttgagc cacagtttca
     5341 cctgcgctgt tttgtacact tgctacattg ccattaaata ctaatttatt tcctgcatag
     5401 attaaatcca catttgtaat cttattcatg tccgctaatt tttggacagt aatatttgtt
     5461 gcttgactga ttgcgcttag ggtatctccc cagacaattg tgtattcatt ccctttaatt
     5521 tctgctttga tttgttccgc cgaacgaggt gtccaaactt tgttttctgc tgcttcagca
     5581 ttcgttccca cagcaagact aactcctccg actacgattc ctagtgccaa tactttcaac
     5641 attttcattg tttttcgcct ccgatttgaa taaatttatt tacaaaaaca tagtaccaaa
     5701 cttatgtcta tcagtcgata tattttttgt agattttttt attttttgat atactaactt
     5761 tggtactaaa ataactaaaa ggaatgtcac acatgcaact taataattta aatgaaatac
     5821 atgattgggc tcaaaataat ttacttaatc gtaaacaagc tgccaaaatc actggacaaa
     5881 actatgatgc tttttctagc tctttacgta gaaataatat caaaccattt ctagaaatag
     5941 gcgacgaacc tggtccttcc atcattcgct tatatctaag agaagatatt gaaaaatacg
     6001 caaaacagct cgaagaaaaa agacagaaac gtcaatgagg cgttttctgt cttggattta
     6061 caagtaaaac aataataaac acttttacaa cggaggttct atgaataaga aatacattgt
     6121 ttcagttggt tcaatcgtac tgattgtact cgctattatt gctttttctg tatccaacaa
     6181 aacagaaaca cagccacaaa atacggttca atcgactact acaagtcaat ctagcgatca
     6241 ttccgtctat ccttcactaa atagtttaaa agaaacgcat ggacaagatt taattgatct
     6301 tggttcttct caaattacta ctgtcttatt tgaaaagaaa aatattgcgt ttattactcc
     6361 taaagaagga actgaccttg aattttctat caaagtaaca aataccaaag aaaatttatt
     6421 ctccattcct gacattcaat atacccctaa caagttaaag attggggata cgtttggttt
     6481 ggctaaagta aatgaacaat actttgctta ctcactgaaa taagtttcta atagcaagag
     6541 gaaaaaccct cttgttattt ttatcacata atcattttat aaaaacgctt cgaaaatatc
     6601 aacatttttg aagaaactat ttttctaatt tctcaatttc ttccaaggga ttttcgctaa
     6661 tgacccatac aggcacagat tgctctcgag gctccccttc ttctaaccac caacctgcat
     6721 aatgaacttc ttcctcctca gcattaccat gataccaacc aacaaaggat tcatctacaa
     6781 cgaatgaaat ttcttcttct ggatcaaata attcattttt ccatgcttca attgcttgtt
     6841 ctttggtgta cttttgtttg ttaaatacac cacaaatttc gtcatctaca taaaaaattt
     6901 caaaatcaaa attactcgtt ccttttactg gcatcttaac ttttcctcct ttgactcact
     6961 tcaatagata taccactcat gttcattcca ttgtttacta gttaattgga gttttatctt
     7021 agggctttgt gcactctttc caaaaacttg agcgtcgttt ttttcttcta accaaaaaag
     7081 gtaatttctt acattcggta ctaaatgttt ttcctgcatt tcttcatgaa aaacatataa
     7141 ttctgcttca tttttcaacc aattcattat tcattccctc attcacttct tcataccgcc
     7201 attcagatag tgagatttct ttttgtttct ccacttgtcc tataaaaatc gctgtattca
     7261 acaagctgat cacacttaat aataaagcaa tgaaactaat ggttacactt actctttcac
     7321 attttgtcat tttgtttcct tctttactcg tttgttattt cttgtaatag gtctaataac
     7381 tccttcaccg aaatttgcaa atggttcgca aaaatttcaa taaaagcact cgcacacatt
     7441 accagacctt caactgatat atcactgcga caataaaatt ctggagaaac actgatttct
     7501 gattctttct tacaattgaa cgaaaaaact atttttgaat caaattccgg taattcttcc
     7561 acaactatgt tttgggattc taccaagaaa gggagtgcct gttctaatgc ttcttctggt
     7621 gtttgagcag atagaataat tgcttcttgt tcttttattt cattgttctc aaatacttta
     7681 taactttttt tcattgttag ttcctctttc attcctctct caattagcta agccgttact
     7741 ggtacgccat tctctaaatt tagtatcgct aatccttaat gctcgtcgaa tatcaatcat
     7801 tcgataacct attgcacgaa gttctttata tcttttagca gttagcgtct ctaacgtcat
     7861 ttcaggcaat gggttcgaat caaaaataga attcgaaccg attatttggg ccaattgttg
     7921 tacttgtaac agctcttctg aacttaattc agtgattgct cgattgccta tcattgcata
     7981 cattttctgt cgaatatact tcttttcttg tttagacaac atttctatcc actcctattt
     8041 tcattatcat ttgttacctc cacttcagta aatccacaaa taaatgaatt catattccaa
     8101 gtaccattcc cttcaactac aacttttctc ctgtcctctt tcggatattc aagaatcaga
     8161 ccgtttacca atatgttttt cactactact aatttgtttg tataccgtgg aattgctgtt
     8221 ccgatatact tcactttatt tcctacgcta attgtcattt cctcactcct attctttttt
     8281 atccaaaact ttttcctgtc ctattcctgc caacgaatat acttcactgc atgcttaaac
     8341 aaaacaaaat gttgccctgt tttatttccg tcttttactt gttccacata cagttcatat
     8401 ttggtcacac cttttaaaac gccagatact ttttttcatc cattgtcaca attagttttt
     8461 tcatcaatcg attgatactg cactgctcat ttttcttgtt catatagtat tcctttcttt
     8521 ttcaaaacta gcctcttttg tttactagtt ttcatcaatc atcaacgatt taacagattt
     8581 tttctttcgc agggtttcta gcgtcagcaa ccccaaactc taaaaaagtg cctttgctcc
     8641 cttgaaaaac gagcggacga ttgtactcgt catcatatgt ttttcatact acctgatcca
     8701 cgcaaataac ctttttttgt tctgtgccac acccatccat acgtatcgct tgagattcac
     8761 catacttttt taggtggttt atccttccta agtccctgtt agacacgctg attaacttag
     8821 aaagtcctgt ttgtatccaa ttttcaaaga tctcccaaaa gaaataagta ggtttacgtg
     8881 tatcgcttct gttcaaagta cctgtttgta cttgagtatt tctactcccc tcgttcaagg
     8941 gattccttcc accagctagc ttttcctttc atcatcaaat gttcctgaaa agcatttacg
     9001 tttcgtgtcc gtgtgcttac ttattctttt ttcgatagcg attatccgct cgctactcgc
     9061 tattttatac tttgtttgtt agctcaaata aaaactaaca aacaaaaaag gctagtttct
     9121 ttccgtttta tttttttaac tgtcgataaa tagttgttag tttaattcca agttctaaaa
     9181 ccaatacgcc caacaaaacc acaggaaaaa caattggggc aaacaagtaa aatcggatag
     9241 ttttcatgtc ttccctcttt ctttttggtg tcgctgtggt cgttcttgcc gttcaatact
     9301 tcgatacatt ttttattcat tatttgctcg ttttgttttc ctctggactg cttttcttgc
     9361 tttttgaata ctttatacgc ttttcgtgcc tgaattaata ggtcttgtcg gcgtttcatt
     9421 cgtgagaaaa caatatttga aaccatcaaa tcctggttcg aacaaccacg attcgcaata
     9481 attttcccat caaaaaaaat agttttcact tgagaaaaaa acagttggaa atcttgtttt
     9541 gttgctaatt tttttgtcaa aatcactgtg cttttccctg ttgaatctac aatgattctt
     9601 cgaacaatcc gattcgataa cttccaacgt tgccgattaa acaatgttac ttcacaacaa
     9661 atatgacgta agtgattagg ggtcattttc atgtgtccaa ttcctccaac caattatgca
     9721 tcgaaatttt gggcaattgt gacagcccat aaatctgcgt ttccaggtct tcataactac
     9781 aagaacgctt ggcttgaagt aatgaatatt ctgcttgatc tgatagtttt gttgccattg
     9841 gatctagatt caattctctt acacaacatt cccaaaaatt caatagcgct ctaaaataaa
     9901 atccttttcg attctgaatt gcttttcctt catgacgagc aattttttca ttttgaatac
     9961 accgttgata cgttttgctt aaatcttctg caaatgtttc ccctaaaatt cgatactcgc
    10021 ttccatcaga taaaccatgt gtttgattat actttcgttg ccgcttttct ataattcgtt
    10081 tactgtcgta tatcatgttt agctgctctt tcgcttcacg ccattcagct gaactgcgaa
    10141 caatccatgt caaaacatct ttcggaacaa acgtactttc gctgtaatga gcaataaact
    10201 ctttttgcca atcatcagcc atttcaacag gactagtcgt atcgattttt gtatctagat
    10261 tttctgtatc tagattgtta gaagaattat taaataattg tgcagaattt tcacatggat
    10321 ttttttcgtc aaaatcatcg aaaatcgcta aattatcaac agattcctcc tcttcttttc
    10381 cttgtgcaga atctacactg gttgagtcaa caggacctaa tgaatacaca tcattttctg
    10441 tcgtatctaa ttctcctaga tacaaccgag ctttgtttct ttttcctgtt tgttgatcaa
    10501 atcccatatc ctctatttct agtagctcat attctttcaa ttcttttttg attgtattaa
    10561 cagaacgtct gcaacaattc aatgcattgg ctaattcttg ttccgtaaat tcaaaataaa
    10621 caaaaccttg actatctact tgatttcgtt gaatggctaa atctaagcgt gaccgaaaaa
    10681 ccatatacgc gatttttgct ttatcagaca atgagcgata tttttcactt gtaataaata
    10741 cctttggaaa ttgatagtat aattcattgt atttacgagt tgcggtaaat ctttttgttg
    10801 ccatcgttat tacacctcat cttgtccata tattaagaaa gaattttggt gttggtagac
    10861 acgaacatct tccattgtag caatcaattc ttttagataa attcgaccga attcaattgt
    10921 tagtaagtcg taatcttcta attcattttt gatttcttca atttcttctt cagacacatt
    10981 gagtaaaatg gctaattctt gattggtata agaaatatag acatgtttct tttcatcaat
    11041 ccgtttttca attaaggatt cttctaagta ctcattcagt aagctatagg cgtattttgc
    11101 ttgtgcagtc atcgcataat attttgacga ttttataaag aatagttttg gcatatgaaa
    11161 agttgcttgt gtcagcacat ctgttgcgcg aaatttgttt tttaccatat tttttcctcc
    11221 tagaaaaaca cgcaaaaaaa gctctgtata aatcgaatta cacagagttt cttcttgttg
    11281 atatttaatt tctaaaatac gtttcgggta aaaactaaac acttactaat atttagattg
    11341 tagaatcttc gccttcattt attcatttta aagagaaaaa gatcaagtga aatgtgccca
    11401 aaagtttgat tctattaatt tttaaacacc attcataata tatttcctat aatgtaaaaa
    11461 gcgtcgatac ttattaaagc atcgacgtta tttttatagt aaatttatca aaatttttgc
    11521 caacattttt tccgattttt ttccctattt ctatctttcc ttatctttct tagttaatat
    11581 tatttgatgt taatatatca ctactaatat taaaaaacat tgatttatca taggataaag
    11641 agtgatagtt taatacttaa agagacatat taattttatt taaaaattga cattaatgac
    11701 tcttaatcag cgggtcgcgg gttcgagccc ctcacggccc attgggtgcc aaacccacgg
    11761 aacaggtaaa tgttggtgcg aaagcatttc gaaaacattt acgctgttct ttttttatt
//
LOCUS       DAJSVP010000075        11608 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSVP010000075 DAJSVP010000000
VERSION     DAJSVP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.078"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(368..1486)
                     /locus_tag="ON449_002063"
     CDS             complement(368..1486)
                     /locus_tag="ON449_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934634.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     NVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="ON449_002064"
     CDS             1957..3264
                     /locus_tag="ON449_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3934635.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="ON449_002065"
     CDS             3383..4582
                     /locus_tag="ON449_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3934636.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="ON449_002066"
     CDS             complement(4609..5877)
                     /locus_tag="ON449_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3934637.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="ON449_002067"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="ON449_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="ON449_002068"
     CDS             complement(7094..7597)
                     /locus_tag="ON449_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3934638.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="ON449_002069"
     CDS             complement(7720..8484)
                     /locus_tag="ON449_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3934639.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="ON449_002070"
     CDS             8592..8867
                     /locus_tag="ON449_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3934640.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="ON449_002071"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="ON449_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3934641.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="ON449_002072"
     CDS             complement(10434..10634)
                     /locus_tag="ON449_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934642.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11439..>11608
                     /locus_tag="ON449_002073"
     CDS             11439..>11608
                     /locus_tag="ON449_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934643.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWH"
BASE COUNT         3509 a         2431 c         2047 g         3621 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacattaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 aacgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg
    11401 tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa catcttacca
    11461 tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg gttgaaatcg
    11521 ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag ttcaagcaag
    11581 gtaagactgc tcttgattat tggcacca
//
LOCUS       DAJSVP010000076        11599 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSVP010000076 DAJSVP010000000
VERSION     DAJSVP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.079"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            264..470
                     /locus_tag="ON449_002074"
     CDS             264..470
                     /locus_tag="ON449_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR3934644.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(672..1673)
                     /locus_tag="ON449_002075"
     CDS             complement(672..1673)
                     /locus_tag="ON449_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3934645.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1678..3590)
                     /locus_tag="ON449_002076"
                     /pseudo
     CDS             complement(1678..3590)
                     /locus_tag="ON449_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            complement(3758..4264)
                     /locus_tag="ON449_002077"
     CDS             complement(3758..4264)
                     /locus_tag="ON449_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3934646.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4424..4864
                     /locus_tag="ON449_002078"
     CDS             4424..4864
                     /locus_tag="ON449_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3934647.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4890..6047)
                     /locus_tag="ON449_002079"
     CDS             complement(4890..6047)
                     /locus_tag="ON449_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934648.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6050..6406)
                     /locus_tag="ON449_002080"
     CDS             complement(6050..6406)
                     /locus_tag="ON449_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934649.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6704..7678)
                     /gene="sgrA"
                     /locus_tag="ON449_002081"
     CDS             complement(6704..7678)
                     /gene="sgrA"
                     /locus_tag="ON449_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR3934650.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7874..8713)
                     /locus_tag="ON449_002082"
     CDS             complement(7874..8713)
                     /locus_tag="ON449_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3934651.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8898..9785
                     /locus_tag="ON449_002083"
     CDS             8898..9785
                     /locus_tag="ON449_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3934652.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10379..11074)
                     /locus_tag="ON449_002084"
     CDS             complement(10379..11074)
                     /locus_tag="ON449_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3934653.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11058..11456)
                     /locus_tag="ON449_002085"
     CDS             complement(11058..11456)
                     /locus_tag="ON449_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3934654.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3456 a         2311 c         1738 g         4094 t
ORIGIN      
        1 taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat
       61 attcccatat tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat
      121 tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat
      181 tttattcaca tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa
      241 ttataaaaaa ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta
      301 gtgcattgat tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa
      361 tattagtgga accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg
      421 caggcttcgg atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat
      481 ttacgtaaaa gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg
      541 ccccttgcca tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct
      601 tgtttattga aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc
      661 atcataagac tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt
      721 ttattgctga aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct
      781 acttttgtga ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc
      841 gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt
      901 ctgaccaagg ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa
      961 gcccatccac atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca
     1021 gaaggatcca acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc
     1081 ggatcaagat gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca
     1141 ctaatttgtg cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct
     1201 tttatctctt ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt
     1261 tttgcttccg gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat
     1321 agatattcca aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc
     1381 atccagaaaa gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta
     1441 ttatttttgt actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt
     1501 tgctggaaat catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt
     1561 ctaacaagca agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa
     1621 ataactacga tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta
     1681 ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga
     1741 ataaatgaga agcgaatgtt tcttttctcc agttttcggc aacattcttt ttttgcatct
     1801 gtcttttttt gtactttcac tgtcatctga atcggttgcg tatcatcact tgattgatcc
     1861 tctcgaggtt cattctttcc cttattgtct gcattctctg tcggatcttg cttttttggt
     1921 ggagctactg ggattttttt gtaatagaaa ataatagttg gtatcgtctc attaaaaatc
     1981 ccttcagtcg gtccttcaac atttgctaat tcgtatcctt cgaatgtctt ttgctctgtc
     2041 atataatttt ctccataagc tccgtaaagc tggatttcgt cctggtcttt gagtagactt
     2101 ttattgtttt catccacata ctttacgttt atctgatcat ggaacactac tttcaaaaag
     2161 tccgttacat gtttcgtgta ggcagcatgg tcaacttcct gtgcctttcc atgccctgct
     2221 ccttctacta ataaaagatc tttataccct cctgataact catataattt ttgcgcgtta
     2281 gacacaggta ctacatcatc tgctgttcca tggataatca atttaggcaa tgaatctgac
     2341 attccaatcg taggtagagc agcttttgta tccaaatgca tctcctgatc aaaatagtat
     2401 tgtttcaaaa ggcccataaa taaatcttca tgctcttgat ccccaatgat gtctaactga
     2461 ttttttatgg catccggtag tgcgggggct actgtctggc tgtacgtaaa acgtagttgt
     2521 tcatcaattg atgcaaatcc gcaattttca ataattcctt tgacattgac aggaatatct
     2581 ttcgctaata cagacatcat cgtagctgct cccatcgaac caccataaag taaaatcttt
     2641 tgagatggcc gctctcgaac ttcttgattt atccagtaaa ggacgtcatc tgactcatat
     2701 tgtccatagg tcacataatc gcctccgcta ttaccagtcg ctctggaatc aacaaataat
     2761 aaattatacc cggccttata aaaatcattg tattcttctg taacgatccc gttattccaa
     2821 ttccctcgaa atccgccatg gatcatgact gttttatctg aaccttgatc gacataatat
     2881 gcatatacat ccatcatttt gccatcttgt ttgtttagta cagtttgatg aagttgtttt
     2941 gtatttggat agaagcctgt gttttctgta ggcgttgtcc catcatctgt acctccaagc
     3001 atcgacatca acacactgtt ttttgcaaaa gcgatatcgt acaatagata agtagcaatc
     3061 agatgtttga taatctcttt atctacacca taatcattaa cagcaagctc agtgactaat
     3121 aactctatta attttgactt gatgtcttct gttggtccat taagtatttc tgtcatctgt
     3181 ttaccgaagt aattcaaata ttctttacca ttgaaatttt cacctttacc gatatcatca
     3241 ttttctgtcc cattctcttt gtcacttact ttagcagaag aggattcgtc gtttgacatt
     3301 tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg tagcggtcgt ttcttgatct
     3361 gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg tttctgtttg tgaagtactc
     3421 gatttcgttt catttccagc atcagtagtt tctgtggttt ctacctcccg agattccgat
     3481 tcggaagaaa tgctctctcc atatactgtc cattgcggga cgcttgtact tacagcaaat
     3541 aagatgacca acgctccctt taatacttgt tttgttctca agctattcac tcctttttat
     3601 gtttctagga gaattatatg taaacgcaaa cattttttat acaaaaaata aatggtttat
     3661 cccataaaaa tagaacagga ttattttctc attttacgta ataaagcaga atggataaac
     3721 cagtatccat tctgcttttt ttaaacgaag aaacaactta ttgctccaaa aatgtcagaa
     3781 atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc ctttgctttc ataaaaggat
     3841 tatcataagt tgtcgttgct cctctacaca tcataagctg gtatcctaat ccatgagcaa
     3901 acttcacagt cgtatctata caatactctg cttgtgcccc acataattcg atcgaatgac
     3961 agtccaattc agttaataaa acactcaaat tagtcttata aaatgaattc gcatgggttt
     4021 ttctcacaaa ataactgtgc tcaggaatag ataattccga caatagctcc catggttttg
     4081 tttgtggcac taattgagca tcttcgtgtt gaacgaaaat aactggtttc ccaatcgttt
     4141 cgtatgtttt gattctttta ttcactaatg ctgtcactgt ttctaattcg tgtatcggcg
     4201 aagaatcaaa acatacccct ttttgtaaat cgatcactac tattgcgtcg gctgtgtttg
     4261 acatacttgt gccttctttc ttttttcttg atttaatagt atttcttatc tagctagttc
     4321 cattatacgt gacaacattt tcttatttta cacctctaaa tggcactttt cgactatttc
     4381 ttcatgttat tatttcataa atacttagct attggaggaa tgtatgatga ttaaattgta
     4441 tacaggttct ggctgctcgt cttcaagacg cgcaaaacgt tggcttatcg tccatgatat
     4501 accgtttatc gaacagaact taattcgtca accactaacc aaaaatgaat ttatccactt
     4561 actaaaattg acaaacaacg gcgtgaccga aatgctctct actcattcta ttagttataa
     4621 aaatcttgtt cacagaatag atgaactcag tttgagtgaa ctatatattg agattcgtac
     4681 atccccttct ttattgaaaa aaccgattat ctacgaccaa tctgcaaaac ttttgactgg
     4741 atttagcgaa gataaaatca ggatgttcct ttctaaaact aaaagaaagc aagagctcaa
     4801 cgaactcttg ctttctggtc atcttgtcga ttttaaagaa aataatcttt ttgcaatgtc
     4861 ttaattttgg cataaacaat ctgcttttct taattaacga aatccaattt ttccaatatg
     4921 gatctatttt tctcattcct gatttttatg atttcagctt tcagacgcgc ccaatcattt
     4981 tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg tttgatcata tgcctttttg
     5041 tcgataggat aatcgctaat gacaatatca ggtaactgat cagcggatgc actatatttt
     5101 attgggcgtt cagtcataga taatatcctt ttctttgccc ataattcctg tgttttacca
     5161 tactttgatt gaatatagaa tatgacaggt tgttcgtgtt ttataaaaat atccctgact
     5221 aacatacagt attgaaaaat caaccgtctg ttcttttcat aataagtatt aagctctgga
     5281 attctgcgag ctaccccact aaaaaactgt tgcatgattg gatacatcac tggaaatata
     5341 tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat ccaccttctt ttttgtccca
     5401 aaaacccttg aatacaaatg gatcattgcc aaattgacat atagataatt tttttctgaa
     5461 gttgtaaaac aaatcttgaa gtttttttca aaattttcta caatagacga ttcaatccag
     5521 ccccaacttt cttgtacttt atctggagta tccaacaaat cttcttccaa ataggtgttc
     5581 attacaccaa acataaaata aaggtaagct tgctctgttg gagtgatgtg attcatcgac
     5641 cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg taaactcttc ttttgttatc
     5701 aatggatgca caaatttgtt catctcttct gggacatacg taatcgagtg gccttgtttc
     5761 attcgttgca acgaaattct tagtaggctg atccatttct ttctatctat actacgcgta
     5821 tatggcgcat gtttcgcaaa tttttctaat agttgacgtt tattttttat acttgataat
     5881 atatcagaat gattttcgtc gaatgactgc caatacatca agtgaaaaaa gtatcggata
     5941 cggtattctt ctccttctat cggattacct tttttcaagc tgcaagataa atgatagttt
     6001 ttcaaaatag tattaagttc tcttaatttt ctttgaatcg tacgcaaact tatataattt
     6061 tttgactaag attttctaag gattgataag tacctttgta aagtgaatcc agtaaaatat
     6121 aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac tgagaagaaa
     6181 actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct ggataccttt
     6241 cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg tagtttagat
     6301 ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa tagttcaaaa
     6361 attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta catactcctt
     6421 tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa tagattcgtt
     6481 actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc ttgctcttat
     6541 tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac aaaaatagat
     6601 ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata ttcactcaaa
     6661 aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc actcgatgga
     6721 agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc atccatgtaa
     6781 ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta ttatctgttt
     6841 ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt tttagcacac
     6901 tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata ggtaccaaat
     6961 caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct gtgctactct
     7021 cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc
     7081 tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgt
     7141 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
     7201 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
     7261 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
     7321 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
     7381 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
     7441 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
     7501 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
     7561 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
     7621 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
     7681 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
     7741 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
     7801 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
     7861 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
     7921 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
     7981 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
     8041 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
     8101 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     8161 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     8221 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     8281 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     8341 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     8401 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     8461 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     8521 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     8581 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     8641 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     8701 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     8761 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     8821 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     8881 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     8941 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     9001 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     9061 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     9121 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     9181 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     9241 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     9301 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     9361 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     9421 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     9481 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     9541 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     9601 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     9661 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     9721 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     9781 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     9841 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     9901 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     9961 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
    10021 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
    10081 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
    10141 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
    10201 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
    10261 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
    10321 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
    10381 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
    10441 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
    10501 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
    10561 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
    10621 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
    10681 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
    10741 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
    10801 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
    10861 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
    10921 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
    10981 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
    11041 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
    11101 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
    11161 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
    11221 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
    11281 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
    11341 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
    11401 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
    11461 gttcctcctc gtaaaatatc atttgtctaa tagtttttat tcaaaatgtt tctagattat
    11521 aaaatttatt gaataacaca ttcctaacgg aatttgtttc gatattgtgt gatttctcag
    11581 agtacaatgg atgtatgga
//
LOCUS       DAJSVP010000077        11534 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSVP010000077 DAJSVP010000000
VERSION     DAJSVP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.080"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(78..2153)
                     /locus_tag="ON449_002086"
     CDS             complement(78..2153)
                     /locus_tag="ON449_002086"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3934655.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2155..3072)
                     /gene="glyQ"
                     /locus_tag="ON449_002087"
     CDS             complement(2155..3072)
                     /gene="glyQ"
                     /locus_tag="ON449_002087"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3934656.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3458..4276)
                     /gene="recO"
                     /locus_tag="ON449_002088"
     CDS             complement(3458..4276)
                     /gene="recO"
                     /locus_tag="ON449_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3934657.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4419..5318)
                     /gene="era"
                     /locus_tag="ON449_002089"
     CDS             complement(4419..5318)
                     /gene="era"
                     /locus_tag="ON449_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3934658.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5333..5728)
                     /locus_tag="ON449_002090"
     CDS             complement(5333..5728)
                     /locus_tag="ON449_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3934659.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="ON449_002091"
     CDS             complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="ON449_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3934660.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6217..8409)
                     /locus_tag="ON449_002092"
     CDS             complement(6217..8409)
                     /locus_tag="ON449_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3934661.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8433..9404)
                     /locus_tag="ON449_002093"
     CDS             complement(8433..9404)
                     /locus_tag="ON449_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3934662.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10109..11488
                     /gene="brnQ"
                     /locus_tag="ON449_002094"
     CDS             10109..11488
                     /gene="brnQ"
                     /locus_tag="ON449_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3934663.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3112 a         2442 c         2127 g         3853 t
ORIGIN      
        1 taataacagc tatttataac aaaaaaaaca gagacacagc acgtttcgtg catgctctct
       61 gtttttcaaa caaaagacta ttttgtgatg atttgatcca agcttgctaa tgacaaagcc
      121 atggcagcta tttgacttag ttgtgttagt cggttcttac gtactgctgg ttcatcagcc
      181 atgaccatcg tttgatcaaa ataattttcg atcagtggac gaagagcgac aagctggtcg
      241 tagttttcgt ggatcgtttg gtcagcaaaa cttgctttca ttgcttctac tgctttgtgt
      301 aattctttct ctgcgtcgtt ttcgaacagc tcagggtcta cttctgattg tttttcaagc
      361 tgcgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccattgaagg tttgaagtct
      421 tcatctgcaa gatgttgttt gaacaattgt gctgctgcaa atacttttgt cagatctttt
      481 tgttccgcat tcaaaacagc atcgatgaca tcatgacgaa tattcttcgt tgtcaataat
      541 tggcgcagac ggcctttgat aaagtcgatc acttgttgtt gcccttctga taaaccaatg
      601 ccgaaacgat ccgtatcttt actgataaca tcgctaatcg tttcttgcag aacagacaaa
      661 ggaaaggccc attttttcga ttcgatgatc cgaacgacac cgtatgcttg gcgacgtaag
      721 gcataaggat cgtttgaccc gcttggaatc atcccaactg caaagaaaga aaagatgcta
      781 tctaatttat ctgcaatcgc taaaacagaa cctactgtag actgaggtag ctctccttca
      841 ctggatatag gcatgtaatg ctcacgaatc gctgtcgcta cagcaggttt ctcgcctttc
      901 agaagagcat atttttcgcc catgatccct tgtagttcag gaaattcccc taccatgttg
      961 gttactagat caaatttata gatttcagca gctcgttgca aatccatcaa ttcttcctca
     1021 gatagtccta cttgttcgcc aatgatacgg gcaatgactt gtacccgttg cattttctca
     1081 tagatactac cgattttttc atggaaagta acagttttta gtttttcaac gtattggtcg
     1141 attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg agccgtcaat
     1201 actttttgat tgcctcgaac cacatcttcc aattttaccc gatcaccatt acgagcagaa
     1261 ataaaatgag gaagcagcat accttgatca ttccgtacct cgaaataacg ttgatgttct
     1321 ttcatagaag tgactagtac ttcttcagga acagaaagat atttttcttc gaagtcgcca
     1381 gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc ttcgtctaga
     1441 tcaataaccc agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt
     1501 cttttttgcg gagacgcaat gacgaattgt tcattcaatt tttcttcata ttcttttgca
     1561 tgcgcaaatg tcacatcttc gcctaagaaa cgatgacctc gagaggctct gcctgttttg
     1621 acatccaaga tttcgaatgg gattacttcg tcatctaaca atgccacgat ccagtgaata
     1681 gggcggatat attcaaaatc gtattctgcc caatgcatcg ttacagggaa agtcaaactt
     1741 ttgatcaccc ctgctaagcc tgtcaatact tcttttgctt ccttaccatg agtgtatttc
     1801 gttacataga catattccac accttttaat tctctaaaag tgatagcgtc tgtcgtcaga
     1861 ccttgtccgc ggacaaatcc ttccgctgct ttcgtccagt ttccatctgc gtccaaagca
     1921 atttttttgg ctggtccttt gacttcttct tcactgtccg cttgtcgttc tggaatattt
     1981 gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat
     2041 tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgaca
     2101 acatgggcag gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc
     2161 agcctccttc acggtttctg gatttgctgt gtttttattc aacaaaggga atcctaattt
     2221 ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga tgcgagaaag
     2281 ataaccggca cgctctgtta cggatactgc accgcgggcg tctaataagt tgaatgtatg
     2341 gctacatttc aagatataat catacgccgg atgaacaaga ttttcatcga tacagcgttt
     2401 agcttctttt tcaaaacgat caaagttgct tagtagcaag tcttggtcgc ttacttcaaa
     2461 agaatatttg gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc
     2521 agcccattct aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa
     2581 tccataagtg atctcagaag ttactggttt acaaggcagt ccaccaactt gctggaagta
     2641 cgtgaattgt gtgatttcca ttccgtcaag ccatacttcc caacccacac cggcacagcc
     2701 catggatggg ttctcccagt tgtcttcaac aaaacggatg tcgtgttcta atggatcgat
     2761 tcctaaaagg cgcaagcttt ctaaataaag ctcttgaata ttctcaggtg aaggtttcat
     2821 caccacttgg aattgatgat gctgatacaa acggtttggg ttttcaccat aacgtccatc
     2881 agctggtcgg cgagatggct ctacgtaagc agcattccat ggttctggtc cgattgcccg
     2941 taaaaaggta tatgggctca tcgtacctgc tcctttttct gtgtcataag cttgcatcag
     3001 catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa
     3061 ttttttactc atctgttttg cccctttcaa ttttaaagcg ttgctgtcaa ataagaatac
     3121 aggaaagaaa acgacaaaaa atccctatgt ctgattacag acatagggac gaataattcg
     3181 cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc
     3241 ctcttcaaaa atcgtttggg tccggctccc actctcccga actcgctaaa ccacgtgttt
     3301 tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttcttgtttc tataatagat
     3361 cctatcactg aacgatctat ttaatctatg gttaataata gacggaactc cctgtgtttg
     3421 tcaaatggtt ttgatttttt gttgtatgtt ttatttacta ttcttctttt tcaggtgtct
     3481 ctttttcttt ttcagacatg tctttctctg acgtctttaa tacgtgtgcc caagacttca
     3541 tttgattgat gaactttttg cttttcaaat ggataccgac atattcatca tacagctggt
     3601 cgatgagttc acggattgct tgtttggttt cttctttcag atggattcca ctaattttat
     3661 cataagaaat agctgaaaac atccgaacga aataaacagc tcgaggatct gcatgataac
     3721 ggtgatcgtc catcgaccaa tgtctttcgc atatcacacc accatatttg gaagaatagt
     3781 caaattttcc ttttgtttcc ccgcatacga cgcagtgtgt ccagattggt gcaattccga
     3841 agcgttgcag tatttggact tcaaaaatat tcgtgatgat ttctgcatcg tctccttggt
     3901 ccatccgttg cagtgcttgt tgcgtaaact ggaacaaatg cgggtcgtac tgatgatctt
     3961 cgattgccgc atctactaga ttcaataaat atgtgccata gccgctcaaa aagatgtctt
     4021 cttggatgtg tcgaaacgca tggacatctt tgacactgct taaaaatgac aaaccttcac
     4081 ttcgaaaatt cccgatataa actgcttcag taaaagggag caaagccgga gctaagggct
     4141 gattttgccg atggatctct ttgatataaa acatcatttt gccagaagat tctgtaaaga
     4201 ttttgactaa tttatctttt tctttgtaat tacggctaga taaaatcaag ccttttgatt
     4261 ctgcaagctg gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg
     4321 atccagatca tttatgttgc gatcttccgc agcttttctc aacacgcgga tttttattca
     4381 gtttatttct atacctaatt tacaagaagc tcagaatatc agtattcgtc ttttcggtag
     4441 ccgaagtctt gcagatagac ttttttgtct cgccagtctt tttgaacttt gacccaaagt
     4501 tctagaaaga ctttgtctcc taaaagattt tcgatatctt ttcttgcttt cgttcctact
     4561 tgcttcagca tttttccgcc tttgccgata atgatccctt tttggctatc tctttcgaca
     4621 ataatcgtag cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg
     4681 actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat
     4741 tcagaaacga tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg
     4801 ccctctggca tttcactgac taatacatcc atcaaacgtt cgacgttgtt tccttctgta
     4861 gcagagattg gaatcacttg tgcaaaaggc atctgcgttg aataatcttc gatgattccc
     4921 aacaactcgt ctggatggat tttgtcgatt ttattgataa tcagataaac aggagaatgc
     4981 acgtttttta gacgttcaat aataaaatcg tctccccgac cgcgtttttg atcagcacta
     5041 atcataaata agaccgcgtc cacttctttc aacgcactgt aagctgcttc taccataaaa
     5101 tctcctaaac gatgctttgg tttatggatt cctggtgtat caataaaaat caactgagct
     5161 tctggtgtag tgtatactcc ttggatttta tttctagttg tctgtgcctt gtcactcatg
     5221 atggcaattt tttgacccac gattcgattc aataatgtgg atttccctac atttgggcgg
     5281 ccgacgattg cgacaaaccc tgatttatgt tctggcatat atgttctcct ttttaattaa
     5341 acaacaaatg atatatttta gggacaaata aaatcaaccc aacgatgacg gtaaaacaag
     5401 cggtcactaa aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat
     5461 gaaaatccgt aaacatgtcc acgacatttt caaaaacagt attgatgatc tctactatcc
     5521 atacgagaaa aacagctaaa agcagccata accattctat ccgatcaagt tggaaaatca
     5581 cgcccataaa caaagcgagg attccgaacg caacatgttt cctcatgttg cgttcttcct
     5641 caaaaacagt tttgactcct tggacggcaa attccacgga catcaggaaa tgtttgtttt
     5701 tctctacgtt tttatctttt aagtccatag gcatctaaga tctctttctg taaaccaaac
     5761 atttctttct catcctctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatga
     5821 accgctaaaa agccaagttc ccgatcatac gaatggccgt aagcttctgc ttgctctgca
     5881 gcacgttcag tagagatcat gatgtctccc agtacacgag gcaatggatt ctctccatct
     5941 tcaaaaatca ctgggatttc gtcttcgccc tcttcttcca aggcaaaact gatgacgtct
     6001 gtcggtgcat cttttccacg gtaatcacga ttgattactt ggattgctgc attgtccatg
     6061 aaagtaacag acatttctgt ttcttctggc aactctaaat atgatgctgc aaattgcagc
     6121 aagtcgtcga tttctttcat tttctcttct gagacttggt ttgtttcatc catgaaggta
     6181 atatccatta tacatcgctt ctttctattc ttcttgctat tcttcttgtt cttcttttat
     6241 cttctcaact tccgatttca ttttaggata tttgatccga gaatggaagg tactgcttaa
     6301 accgctgatc aatgattttt ctactttacg gatttctttt aacgtcaatc cactgtcgtc
     6361 caattgtcca tcggaaatac gctcttcaat aagattatgg acaaaacttt cgattttttc
     6421 gctcgttgga tggtccattg cccgtactgc cgcttcacaa ctgtcagcta tattgacgat
     6481 tcctgcttca cggctttgag gttttggtcc aggataacgg aattgctctt ccgtaacttc
     6541 cgggttgcgt tcctttgcct taacgtagaa ataacgcatc agcgtggttc catgatgctg
     6601 tctacaaata tcgatcacca tttgcggcat attataatct tccaatattt tggcaccatc
     6661 tgtcacatgt ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcggatt
     6721 ttccgctcct gaaggcaagt tctcaacgaa aaagctggcg tgtttgatct tgccgatatc
     6781 atgataatag caagctactc gagttagtaa agaacgtcca ccgatttctg ctacagcatt
     6841 agcacttaga ttggctacca tcatgctatg gtgatacgta cctggtgctt cttccagcaa
     6901 ctgtttcaat aaaggatgat tgggattgct gagttcattt aatacgatca cactatcatc
     6961 attgaccatc aattcaatat aggggtgcag acctatcgtc agcaagaatg aaaacagtgc
     7021 acctgtgaat ccgcagacta gcgttaacca agtcgttgca tcaccaaagc tcattccttg
     7081 atagacgatc aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga
     7141 taatccttgt tctgagatcc gtttacgtct gacaatcgtt gccatgagcc cagagaaaac
     7201 ataagacatc aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag
     7261 tgcggatact gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct
     7321 taaaacaagt ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt
     7381 ttggaataat tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa
     7441 aagaacgtat tttgttcttt ctcctgtatt ttggaattga agtgaattat aaatcagtac
     7501 gccgatttga agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg
     7561 attagtcagt cctagtaatt ccaatttttt catagctgaa gcatcaattt gattaccttc
     7621 acgaacaatg atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg
     7681 tttcaattct tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac
     7741 taagtagcga atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc
     7801 tgcttcttgt ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc
     7861 aatgagcttc agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat
     7921 cgtcgaataa aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt
     7981 ttcaaaattt ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc
     8041 atcttccttt gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt
     8101 atcgatcaat tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg
     8161 gtaagtgtat tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc
     8221 cgtgttctcg atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa
     8281 atcattggcc ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa
     8341 gaacagcagg aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt
     8401 attcaacatg tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca
     8461 taagctttga tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta
     8521 acaaaatcga ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtt
     8581 gtccctcgag gcagatcaat ctgactcgta tctccattga caatcatttt tgattgataa
     8641 ccaagacgtg tcaggaacat tttcatctga gcaactgtcg tgttctgtgc ttcatccaaa
     8701 ataacgaatg catcatccaa tgtccgcccc cgcatgtaag ctaaaggagc aatttcgatc
     8761 acgcctcgtt ccatcaatcg gttcgtatga tctaatccaa aaatttggta taaagcatca
     8821 taaacaggac gcagataagg atctactttt tcttttagat ccccgggaag aaagcctaga
     8881 ttttcccctg cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc
     8941 aaggcagcga ttgctaatac tactgcaaga aaagtttttc ctgtcccggc tggtcctacg
     9001 ccaaatacga tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt
     9061 acccgtatag gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa
     9121 taatctaatg tgcctttttg ccccattttc agtgccgtca cgacatctgg tgtacttaca
     9181 tggattcctc gtttgatcaa ttcctgcaat gtccgaatga ctgaggcagc caaggaaatc
     9241 tgggattgtt cgccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttctgtt
     9301 gtttcttcaa tcagttttat gtgcttgtcg tgtgcaccta aaagcatgct gacatcatca
     9361 gcagccgtca atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt
     9421 tttttcgatt cattctttcc tattttacca tactcagaag aaactatata gcaaataaga
     9481 aaaggttcta aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa atagctgctt
     9541 ccattctcta aaggtctcgt ttgaataaaa aaagctgatc atgtaagcta tgcataagat
     9601 agaggacaag tattccgtct aacttagcaa gtattctctg ggtatttttc ttgtttcaaa
     9661 aaaagaaaac aaaatatttg aaatattctt gcatttcaaa aaaaacaggt gtattataac
     9721 gtcaacaaat aaatatacag caaagcgtcg aggaaaacag taggctattc agtgatttat
     9781 tcccagtgag tcatgtaagt gagaaatgac aataaatcga atagacgaac atcatttccg
     9841 agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat tatcaggcca gcggataact
     9901 ttatcgaaaa gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc
     9961 tagaatctag agcccttttc ccaagtggaa aagggctttt tttatatttt aaggtggtgg
    10021 ttttcatgga atctgatatt agtggtattt ctagtttctg caacgtttat tttgttactc
    10081 aaaatgataa ttattggagg aaaaatttat ggaagaaaca aaaacttatt catggaagca
    10141 attattgatg gtcagttcac ttatcttcgg gatgttcttc gggtcaggta atctgatctt
    10201 tcctgtccac ttaggtcaaa tggctggatc aaactggatc agcgctggtg taggatttgc
    10261 catctcaggc gctcttttcc cattattagc tatcttagct gtagtagtga caaaaagtga
    10321 tggtctgtat gacttagcta aacctgtcgg aaaatggtat gctgccttgt tcttgatcct
    10381 tgttcattta acgatcggac cattctttgg tacaccgcgg acagcagcaa ctgcttttga
    10441 aatggctgct aaaccctttt tgcctgaaaa atatgattca ttaggtatgt ttttattctc
    10501 tgctcttttc tttttacttg cttatttcct tactgtacat ccaacacgtt tggtcaaata
    10561 tgtcggaaag tatttaaact ccttgtttct tgcattatta gctatcattt ttgtcgtttc
    10621 tttattccaa cccatgggga atctaaacca atctgctgca gattcttatc agacgaacgc
    10681 tttgacaaat ggcgttttag aaggatacaa cacggttgat gcagtagctt tacttgcttt
    10741 aagtgtcact tttgtccatg cagttcgtga tcttggtttt aaagataaaa aagtcacaga
    10801 attaacagca aaagctggta gcctgagcgt tgcgttagaa gtcatgattt attttggtct
    10861 ggtccttctc ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg gcggaactgc
    10921 actttctcag atcactaatt tttatttagg taacttcggt gctagcttct taggggtaat
    10981 ggtcacatta ggtgtcttca ctaccgctat gggactcgtt gtttcttttg cacaagattt
    11041 ccataaactg ttccctaaag tcagttatat gacatggctg cggttaacga cttttgtctc
    11101 ttttgtcgtt gcgaatgccg gattggataa tatcatccaa tggtctttac cagtattgat
    11161 gcttttgtat ccattgtcac ttgcgttaat cttgctttcc ttaacagcaa aattcttcca
    11221 aaagagtcca tttgtctatc aagtaacgat gttcttttct gccgttccag ccgtattgga
    11281 tatgttggca aattcgcctg cattagtgag tcagcaaact gtcgtagcaa gcttactagg
    11341 attttatcat cattatgttc cttttgctgc attaggactt ggctgggtcg tcccaacgtt
    11401 gcttggttac gctggcagct tgctctttta ttatgtatat cgcgtatcag gatataaaca
    11461 agaagcaagt gagttaccag aagaataacc aaatcaaaca caatttaaca taagagatat
    11521 taaacaggaa aaaa
//
LOCUS       DAJSVP010000078        11339 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSVP010000078 DAJSVP010000000
VERSION     DAJSVP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.082"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..1024)
                     /gene="nrdF"
                     /locus_tag="ON449_002095"
     CDS             complement(59..1024)
                     /gene="nrdF"
                     /locus_tag="ON449_002095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3934664.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1074..3233)
                     /gene="nrdE"
                     /locus_tag="ON449_002096"
     CDS             complement(1074..3233)
                     /gene="nrdE"
                     /locus_tag="ON449_002096"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3934665.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3392..3616)
                     /gene="nrdH"
                     /locus_tag="ON449_002097"
     CDS             complement(3392..3616)
                     /gene="nrdH"
                     /locus_tag="ON449_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3934666.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4017..4490
                     /locus_tag="ON449_002098"
     CDS             4017..4490
                     /locus_tag="ON449_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3934667.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4487..6619
                     /gene="feoB"
                     /locus_tag="ON449_002099"
     CDS             4487..6619
                     /gene="feoB"
                     /locus_tag="ON449_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3934668.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6621..6755
                     /locus_tag="ON449_002100"
     CDS             6621..6755
                     /locus_tag="ON449_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3934669.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6849..7493
                     /locus_tag="ON449_002101"
     CDS             6849..7493
                     /locus_tag="ON449_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3934670.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7680..8873
                     /locus_tag="ON449_002102"
     CDS             7680..8873
                     /locus_tag="ON449_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3934671.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8866..10593
                     /locus_tag="ON449_002103"
     CDS             8866..10593
                     /locus_tag="ON449_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3934672.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            10987..11184
                     /locus_tag="ON449_002104"
     CDS             10987..11184
                     /locus_tag="ON449_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR3934673.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            11186..>11339
                     /locus_tag="ON449_002105"
     CDS             11186..>11339
                     /locus_tag="ON449_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3934674.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLI"
BASE COUNT         3581 a         1988 c         2346 g         3424 t
ORIGIN      
        1 atatctgtac gctaaactct ttttattttt cgaaaaattt ttgaattaaa ataaatcctt
       61 attccaatcc aatcaaataa tcgtcgtctt tcatcgcttc tacatgacct aacaaatagc
      121 cgtttccgac ttgtgagaag aagtcatggt tgcttgtgcc tgttgaaatc ccgttcatta
      181 cgattgggtt gacatcattg gcagtatctg gaaagagtgg atccatccct aggttcatca
      241 atgctttgtt ggcattatag cgtaggaatg tcttgacttc ttctgtccag ccgattggat
      301 cgtataattc ttctgtataa cgttcttcgt tttcgtaaag ctcataaaga agattgtaca
      361 tccagtcttt taacgtttct tgttctgctt caggtaactc gttgaatcct aattggaatt
      421 tgtaaccgat gtaggttcca tgtacagatt catctcggat gattagtttg atgatctctg
      481 ctacgttagc caatttgttg tttcctaaat aataaagagg agtataaaaa cctgaataga
      541 acaagaatgt ttctaaaaag acgctggcaa ttttcttttc tagcggtgta ccgtgtttgt
      601 agatttcatt gatacgctca gctttttttt gcagataagg attcgtattt gtccagtcaa
      661 aaatttcttc gatttctttt ttcgtattca gtgtactaaa aatagaagaa taacttttgg
      721 catgaacgga ttccatgaat tgaatattgt tcaaaacggc ttcttcatgg ggagtgcgga
      781 catctttacg caactgatcc atgccacttt cagattggac agtatctagc agtgtcaaac
      841 cgccaaatac atgtcctact gtatcttttt ccaattgaga caatgtacgc caatcatcta
      901 ggtcatttga aagtgggata cgtgtatcta gccaaaattg ctctgtcagc ttctcccaag
      961 tggatttatc aatgatatct tcaatttcat tccagtttat tgcttcataa tatgtttctg
     1021 acatgagaat gctcctttaa ttaaaatatg aataagcgaa tctatctatg atattagatc
     1081 acgcagcttt cacattgatt gcttccgatt tcttctgcat catcagtaaa ggtacgtacg
     1141 taataaatgg atttgattcc tttgtggaaa gcgtaatgtc ttaaaatatt caaatcacgt
     1201 gtcgtttgct tcgttgttgt cttccattca taaatgcctt ctgggatttc agaacgcatg
     1261 aataaagtca aactcatgcc ttgatcgacg tgttgttgtg ccgttgcata aacatcgatt
     1321 actttacgca tgtccatatc ataagcagaa gtgtaatacg gaatcgtctc attggataga
     1381 taaggcgctg ggtaataaat tttaccgatt ttcttctctt ggcgttcttc gatcatgcga
     1441 gtgataggat gaatacttgc actcgtatcg ttgatatatg aaattgagcc atttggtgct
     1501 actgccaagc gattttgatg atacagtcca tctttcatta ctgcttgttt caaagcattc
     1561 caatcttcag cagttgggat aaaaatgcct tcaaacaatg ctttgacttt ttctgatttt
     1621 ggctggaatt ctccagttac gtatttttcg aaataagtgc cgtcagcata agctgatttt
     1681 tcaaaattat ggaaaacttg ttttctttca cgagcgatat tgttgctttc aacaagtgtc
     1741 caataattca acagcatgaa gtaaatattt gtaaagtcta atgaatcctc tgagccatat
     1801 tccatttgtt cttttgcaag ataagtgtgt aagcccatgg cacctaatcc aatcgtatga
     1861 ttcagcttat ttccgttttg gatcggtggt acgacatcga tatcagatgc atctgtaata
     1921 aatgtcaatg cacgagtcat cgtacgcaca gattgaccaa agttagggct ttgcatcaaa
     1981 ttcacgatat ttgtcgagcc taagttacat gagatatctg ttcctaatac ttcatattcc
     2041 tgtttgccgt taatcacaga aggcgtttga acttgtaaaa tctctgaaca taaattactc
     2101 atgatgatct tgccatcgat tggattctcg cggttcgctg tatcaatatt gattacgtaa
     2161 ggatagccag attcttgttg taattttgag atttcatttt caagatcacg cgctttgatt
     2221 ttttgtttgc gaatgtttgg atttgcaact agatgatcat actcagctgt aatatttaca
     2281 taagagaatg ggacaccata ttctttttcg acactatatg gactaaataa atacatatct
     2341 tcgtttttac gtgtcaattc ataaaattta tcaggtacga tcacgccaag agaaagagtt
     2401 tttacgcgaa tcttttcatc tgcattttct ttttttgctg aaaggaaagc aataatgtct
     2461 ggatggaaaa cgttcaaata aaccacgcca gctccttgac gctgacctaa ttgattagaa
     2521 tagctgaaac tatcttcgaa taatttcatg actggaacga caccacttgc agcaccctca
     2581 tatcctttga taggagcgcc ggcttcacga agatttgata aagtaatccc tacgccgcca
     2641 ccaatacggg acaattgcaa agcagagttg attgagcgcc cgatagagtt catatcgtca
     2701 gtgacttgag tcaagaaaca agacaccaat tcgccccgac gtttacgtcc agcattcaag
     2761 aaagaaggtg tagctggctg atatcgttga tggatcattt cgtctgcaag agcaagtgct
     2821 aattcttcat ccccgttcgc aaagtaaagc gcattgaagg ctactcgatc ttcataagtt
     2881 tccagataag cggaaccgtc gttggttttc aacgcatatt gagaataaaa tttgtaagca
     2941 gccataaaag atttgaaaga aaaatgttgc tcgtccaaaa attgatatag cttttcaata
     3001 aatgatggtg aatacaattg aatgaattct gcttctatat attcttgatc gatcaagtaa
     3061 ttgatttttt ccataataga tggaaaagtc atcgtatttg gtttgacatt ttcctcaaaa
     3121 aaggcaacta aagcttcttt atccttattc aaagggattt gaccattgac tggccgattg
     3181 atttcattat tcagcttgaa ataactgaca tcttgtaaat ttttaagact catacgtacg
     3241 cccaaccttc atttcgtatt tgatgatgct tgattgcaaa tcattgcatt gtttttaagc
     3301 tttaaaaaag gatttggaag tttgcgacaa aaactggtgc tgtctcaagc ccttcaaatc
     3361 catgtatcac gttttgattc ttttagtcat gttagctggc aagtttacgc aactgatcag
     3421 gacggaatcc gataactgtt tctgcatcag aagtaatgac aggaacactt tggaatcctt
     3481 gtgctttcaa ccagtctact gcttcaggct gttcatcgat attgacttct tcaaaagcaa
     3541 tttgattttc tgttaaaaac cgtttgacca tcttacattg catacaatta tttttagaaa
     3601 agatttttac gttcatggtg agtgcctcct ctaacacatt cttattatct ataatagata
     3661 gtataaaact cttagtaaaa aagtcaaaca acaaacacta catattgtgt tgtggaaact
     3721 gtggaatact aagttttgtg tttttgaaaa aagagctgaa aacaagtatc gtgtgggaaa
     3781 atagcaaatt taatagagaa aaaattgtgg aaaactatga agaaatattt ttttctcatt
     3841 tttatctatc aatactaaag attcgctcat aatatcacaa gtataattga cattctcaat
     3901 taccgagagt attctatgtg agaatgattc tcatttattt gcgtaacacg taagaaaatt
     3961 agaacatata atgaatgatt ttaatacatg atttatttca caagagagga atcaatatgc
     4021 agaccttagc gcaggcaaag cagaatttcg tttatcaagt cgaacgaatc gaaacagaag
     4081 aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc cggaagtaaa atcgtcgtcg
     4141 tcaatttatc aggcaacaat gccattttac tggtaaaaag tcatcgagtg gcagtaagtc
     4201 gggaaatact aggtcaaatc tttgtcaata aaaagactgc ttcctctgaa agctggactt
     4261 ctcttgatca attaaaagca ggagagactg ggatcgtcgt agcaatccac ggacaaggcg
     4321 cagtgaagcg acggttaatg gatatgggat tgacgcgaaa tgtaaaaata gttatccgaa
     4381 aactagcccc tctgggagat ccaatcgaac tgacagtgcg agggtatgaa ctgacgttac
     4441 gaaagtcaga ggccgaacta atcttgatac aaagggagga aaaagaatga atatcgcgtt
     4501 agtcggaaat ccaaacagcg ggaaaactag cttgttcaat ctcttgactg gttcgaatca
     4561 atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa gaaggatcgt tgaaaaaaaa
     4621 tccgaacatt catattcagg acctacccgg catctattct ttgtctcctt atacaccaga
     4681 agaagtgatt gcaagagatt atttggttca ggaacagcct gaactgatca tcaacattat
     4741 tgatgctacc aatttagaac gaaatcttta tttaactacg cagttattgg atttagacat
     4801 tcctatgatc attggtctga atatgatgga tgtcatcaaa aaagaaggaa aaaaaatcaa
     4861 tctagaaaag ctttcctatg gtcttggtgt accagtttta ccaatcagcg tattgaaaaa
     4921 gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct gcaaaaaagc ggccttctat
     4981 tcctgttccc tgtacatatg atccacgttt agaagcagct ttacatgaaa ttgaagatgt
     5041 actaggagaa caaagcggca agaaacgctg gtatgcaatg aaatattttg aacgagatca
     5101 gaaggtaaga gaggattacg agatagcagt ttctcagcaa aaagagatcg agcaactgat
     5161 ccagttaaca gagaaactat tggacgacga tagtgagacg attctggtca atgaacgtta
     5221 tgaatttatt acccaactat gtacactctg tgttgtgtca aatgattctt tccaattatc
     5281 gatgagtgac aaaatcgatc agattgcaac gaatcgttgg ttagccttgc cgatcttcgc
     5341 atttgtgatg tggctgatct attatctggc tattcaaaca gtaggaacaa tggggacaga
     5401 ttggatcaat gacacattgt ttggtacatg gttaccagaa catgttggca gattgttagc
     5461 tgaatggcaa gtagcgggat ggatgcagga gctgatcttg aacggcatca tcgcaggcgt
     5521 gggagcagtg ttaggatttc taccgcaatt gattatattg tttttatgct tgagctttct
     5581 ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg gatcgacttt tcaggaaatt
     5641 cggtctttca ggaaaatcct tcattcctat gctgattgct acaggatgtg gagtgccagg
     5701 cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt cgtttgacga tcatggtcac
     5761 aaccttcatg ccttgttctg caaagttgcc gattattgcc ttagtagcag gggccttttt
     5821 tcctcatcaa tcttgggtag ctccttctgc ctattttctt ggtatgactg caattatttt
     5881 ctctggaatc agcttgaaaa aaacacgttt gtttgctgga gatacagctc cttttatcat
     5941 ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc ctaagacaaa cctatgaccg
     6001 cagcaagtct tttgttaaaa aagcaggaac gattatcttt gtttctagta tcgtgatctg
     6061 gttcagctct tcttataatt tttatgctca acctgtttct gaagaccaaa gtatcttagc
     6121 ctttttcggg cgaatcctgg cagttgtttt tcagccgctt ggttggggaa actggcaagg
     6181 aacagtcgca gcgattacag gattggtcgc taaagaaaat attattggca catttggtat
     6241 tttgtttggt catgcggaag tatccgaaaa tggcagagaa atctggcaag tgctacaaca
     6301 cacatatact cccgcagcag cttattcatt gttagccttc aatttacttt gtgcgccttg
     6361 ttttgcggca attggggcta tccatcggga aatgaatgcc aagaaatgga cttggatcgc
     6421 cataggctat caatgcggtt tagcttattt agttagtttt gtgatttatc agttgggaca
     6481 cttattggag ggtggacagg tagcgttagg gacgtatctt gctattttac tgatgatagg
     6541 actcgtttat gttttgttcc gtcagccaaa atccaagaac cagttttata ctatattgtc
     6601 attagaagga gaggaatagg atggcgacat tcttattaag tgtgttaatc tttggaagtg
     6661 caggcgcaat tgtctatact cgtttaaaat ccggtaagaa ttgtgatgac tgtcaaacag
     6721 cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt cgttgcaaga ggctattctc
     6781 cccttttttg cttattttgc tagagcatag taagatgagg gaaagtaaaa aaagaaagag
     6841 gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg ctaccagcag attgctgttg
     6901 atttagccga acgtatcgtt gaaaaccgat ataaagtggg tgagaagatc catgcccgct
     6961 caacacttgc cagtacgtac aatgcttctc ctgagactac ccgcaaggct atcaatgtgc
     7021 tggtagatct tggaatcatg gaagtacggc atggaagtgg ggcatttgtt gcttcgaagg
     7081 aaaaagcaaa agatttccta ttgcaatatc aagatgtaca atccatccaa gatacaaaag
     7141 cagaaatcat ggcaagtgtg gagaagcaac aagatgagct gaatcatttt tctcaattat
     7201 tggatcagct ggtcaatcaa acgaagcgaa tacataagat gaatccttta cttccgtttg
     7261 aattatcttt atcagaaaaa gcaatgcatc tggaccgttc aattagcgag ttgaatatct
     7321 ggcaggcgac tggcgccaca gttatcgcaa tttctcataa tgaagaactg cttgtttcac
     7381 ctggccccta tgctaaattt acggcagggg acatcgttca ttttattgga aatgaatata
     7441 cactacagcg tatgacaaac tttttttacc cggagcatca gcttgcagaa tgattgtgtg
     7501 aaatattaaa aaaatattaa cttggctaaa ccttttattt acaaagattt agccaagttt
     7561 ttttcagttt ggacaagcaa atttctttca tttttgacaa agtgactaca tgatgttaac
     7621 atagtgtggt cacttttttc agaaagaaaa atgaatttac ataaaaaagg agaatttgtt
     7681 tggtaaaagt agaagtaaaa catttaacaa agatatttgg taaaaagaca caagccgctt
     7741 tagatatgat gaatgatcac cagccgaaaa cggagatttt aaagaaaacc ggtgcaacgg
     7801 ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga aatcttcgtg atcatggggc
     7861 tttccgggag tgggaaatcc acattgatcc gtctattgaa tcgtctgatt gagccaactt
     7921 cgggcagtat ctatattgat ggagaagatg tatcgaagtt aagcaaagaa gaattgcgag
     7981 aagtccgtcg tcataaaatc aatatggttt tccaaaactt cggtctgttt ccacatcgaa
     8041 cgatcttgga aaatacagaa tacgggttag aagtccgtgg tgtacctaaa gaagaacgac
     8101 aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct ttcatttaaa gaccaatatc
     8161 ctagtcagtt atctggtggg atgcaacaac gggttggcct agccagagct ttagcaaatg
     8221 atcctgagat cttgctgatg gacgaagcgt tctcggctct tgatccgctg atccggcgag
     8281 aaatgcaaga cgaattactg gacctgcaag ccaatgttca aaaaacgatt attttcatta
     8341 cccatgattt gaatgaagct ttacggattg gggacagaat cgccttgatg aaagacggcg
     8401 aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa tccagcaaat gattatgtac
     8461 gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac ggctcaaaac atcatggtac
     8521 ctgcattgac gacgaatatc gaatctgatg gaccaaacgt tgcattgaca aggatgcgta
     8581 atgaagaagt cagcatgctg atggctgttg acagaaagag acacctgaaa gggatcatca
     8641 ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc attgatcgac ttcctagatg
     8701 aaaatgtgac ggtgattggg aaagacatgg tggtaagtga tattttcaat attatttatg
     8761 actcaccgac tccacttgca gttgtagagg atggaaaatt aaaaggtgtc gtcatccgag
     8821 gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag tgaacatgaa taatctattg
     8881 aactatcaat taccagtggc aaactgggta gaaaatatca cagaatggtt tacgacaact
     8941 ttttctggcc tattttcatt tttacaaacg attggacagg cagtgatgtc ggggattaca
     9001 aatttactgt tagtgattcc agctcctttg tttatcttgt tattgacggt agcagctttc
     9061 tttatttcta agaaacgtcc aggacttacg ttgttcacat taatcggtct atggtttatt
     9121 tataaccaag gtctatggaa tgatttgatg aatactgtca cacttgttct tttatctagt
     9181 gtgatctcga tcatcattgg ggtaccatta gggatcttga tggcaaaaag cagtaaagcc
     9241 caaagtatta tcaaaccgat ccttgacttt atgcagacaa tgccaggatt cgtttatttg
     9301 attccagcag ttgcattttt cggtatcggg atggttccag gggtatttgc ctcagtcatc
     9361 tttgctctgc caccaaccgt tcgttttacg aatttaggga ttcgacaagt tcctaaagaa
     9421 ttagtagaag cttctgattc attcgggagc acaagtcgtc aaaaactatt caaactagaa
     9481 ttgcctttag ctaaaagtac gatcatggca gggatcaatc aaacaacaat gctgtcactt
     9541 tcaatggtcg ttattgcctc tatgatcggt gcacccggct taggacgcgg cgtattatca
     9601 gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg gggttgcatt agttatctta
     9661 gcaatcatca ttgaccgttt tacacagcat ttgaaccagc caaacaataa aaaagctgca
     9721 ggagcaacaa cacagaaaag taaaaaacgt caaggactga tcattggtgc tgtagtagta
     9781 gtgatcttag gtgcgattgg tatagggagt ttttcatctg ccaaagaaac aaaacgtatc
     9841 aacttatctt atgttgaatg ggatacagaa gttgcttcaa caaatgtagt cggtgaagta
     9901 ttgaaacaaa tgggctatga tgtaacgatg acgccattag ataattcaat catgtggaaa
     9961 tctgtttcaa atggcgagtc agatgccatg gtttcagctt ggctacctaa aactcatggt
    10021 tcacaatatg cacaatataa agatcaagtc gaagacctag gtgccaattt gacaggagca
    10081 aaagttggtt tagcagtgcc agcctacatg gatgtaaatt cgatcgatga gttaaccgat
    10141 caggcaggta agaaaatcat aggaatcgag ccaggagctg gtgtagttac ggctgctgaa
    10201 aacacgatcc aaaaatatga caacttgaaa gactggaaag tagagacatc ttcttcagga
    10261 gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg aaccaattgt agttacagga
    10321 tggacacctc attggatgtt tgccaaatat gatctgaaat acttagaaga tcctgaaaac
    10381 ggaatgggaa gcgaagagca gatccatacg atggttcgaa aaggattgaa agaagaccag
    10441 ccagaagctt acaaagtatt agacaatttc cattggtcag aaaaagatat ggaaaaagtt
    10501 atgttggaaa tcaacaatgg aaaagatcca cagcaagcag caaaagactg gatcaaagag
    10561 aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca gacacgaaat aaatggagct
    10621 tccaaaattg gatcaaatat ccaaatttgg aagctccatg ttcgtctcta tcttttttgt
    10681 ctataaaaaa agagaatgcg tagctcatct tcgaaacgtt caaagacgcc gacaaactat
    10741 tatgaattta tgggtgcgag tcaaagagat cgatcgtagg tatgaaagac taatgtgatt
    10801 aaagtttatt atgtacgaag tgcattctca aaaaatatta aagaatatac atagtttata
    10861 tacttaaaag tgattaaatt cttattcagg aattattttg ctacgtttca agaaggaatt
    10921 gttttttgaa atttaatgaa tgttagaata atagtctaaa aacattaatt taggggtgat
    10981 tagattatga aacatttaaa aattttgtct attaaagaga cacaacttat ctatgggggt
    11041 accactcata gtggaaaata ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg
    11101 gtcgattggg ccaaggcaac tacttgtatt gcaggaatgt ctataggtgg ttttttaggt
    11161 ggagcaattc cagggaagtg ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt
    11221 atataatgat atatctataa gtaaagatcc taaatattct gatattcttg aggttttaca
    11281 aaaggtatat ttaaaattag aaaaacaaaa atatgaatta gatcccagtc ctttaataa
//
LOCUS       DAJSVP010000079        11146 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSVP010000079 DAJSVP010000000
VERSION     DAJSVP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.083"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(210..362)
                     /locus_tag="ON449_002106"
     CDS             complement(210..362)
                     /locus_tag="ON449_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934675.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(671..1084)
                     /locus_tag="ON449_002107"
     CDS             complement(671..1084)
                     /locus_tag="ON449_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3934676.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(1161..1457)
                     /locus_tag="ON449_002108"
     CDS             complement(1161..1457)
                     /locus_tag="ON449_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934677.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(1454..2011)
                     /locus_tag="ON449_002109"
     CDS             complement(1454..2011)
                     /locus_tag="ON449_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3934678.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(2008..2427)
                     /locus_tag="ON449_002110"
     CDS             complement(2008..2427)
                     /locus_tag="ON449_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR3934679.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(2424..2642)
                     /locus_tag="ON449_002111"
     CDS             complement(2424..2642)
                     /locus_tag="ON449_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934680.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(2629..2985)
                     /locus_tag="ON449_002112"
     CDS             complement(2629..2985)
                     /locus_tag="ON449_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3934681.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(2985..3290)
                     /locus_tag="ON449_002113"
     CDS             complement(2985..3290)
                     /locus_tag="ON449_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934682.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(3287..3448)
                     /locus_tag="ON449_002114"
     CDS             complement(3287..3448)
                     /locus_tag="ON449_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3934683.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(3445..3747)
                     /locus_tag="ON449_002115"
     CDS             complement(3445..3747)
                     /locus_tag="ON449_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934684.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(3740..3904)
                     /locus_tag="ON449_002116"
     CDS             complement(3740..3904)
                     /locus_tag="ON449_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934685.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(3909..4178)
                     /locus_tag="ON449_002117"
     CDS             complement(3909..4178)
                     /locus_tag="ON449_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934686.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(4190..4939)
                     /locus_tag="ON449_002118"
     CDS             complement(4190..4939)
                     /locus_tag="ON449_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934687.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(4942..5628)
                     /locus_tag="ON449_002119"
     CDS             complement(4942..5628)
                     /locus_tag="ON449_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934688.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(5634..6305)
                     /locus_tag="ON449_002120"
     CDS             complement(5634..6305)
                     /locus_tag="ON449_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3934689.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6298..6639)
                     /locus_tag="ON449_002121"
     CDS             complement(6298..6639)
                     /locus_tag="ON449_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934690.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(6817..7515)
                     /locus_tag="ON449_002122"
     CDS             complement(6817..7515)
                     /locus_tag="ON449_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3934691.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7602..8072
                     /locus_tag="ON449_002123"
     CDS             7602..8072
                     /locus_tag="ON449_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934692.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8115..8294)
                     /locus_tag="ON449_002124"
     CDS             complement(8115..8294)
                     /locus_tag="ON449_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934693.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8281..8619)
                     /locus_tag="ON449_002125"
     CDS             complement(8281..8619)
                     /locus_tag="ON449_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3934694.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(8635..8838)
                     /locus_tag="ON449_002126"
     CDS             complement(8635..8838)
                     /locus_tag="ON449_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934695.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(8853..9629)
                     /locus_tag="ON449_002127"
     CDS             complement(8853..9629)
                     /locus_tag="ON449_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3934696.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            9928..10248
                     /locus_tag="ON449_002128"
     CDS             9928..10248
                     /locus_tag="ON449_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934697.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10266..10697
                     /locus_tag="ON449_002129"
     CDS             10266..10697
                     /locus_tag="ON449_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3934698.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            10827..>11146
                     /locus_tag="ON449_002130"
     CDS             10827..>11146
                     /locus_tag="ON449_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HCR3934699.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEK"
BASE COUNT         3114 a         2254 c         1724 g         4054 t
ORIGIN      
        1 tttgaaataa taaaagaatt tggggcatat ataaagctac aaaaatcttt ttggttagtt
       61 aaaactaatt tgaatccaaa tcaaatgtgt gaaaaattaa atacagtact tgataataac
      121 gattccttat tcatctgtga actgcaaaaa aattatcaag gtagagctac agaggaaaat
      181 tggaaattca ttaacgaaca tattttctct tagtaaggat tagatatttt ttctctgttt
      241 aaacaattgc aaataccgtc aatatctgaa ttagaaacac ttaccttttg ctccttgcta
      301 ctaccaatag cttggagcaa ttcttgtatt tcttctggag tgccttctac tgataatttc
      361 atttttcttc cctccaatac ataaattaat agacagcagc agttgaaaga cgacaagaac
      421 atgtttagtt ttgtgagtgc tgctgtctaa tcaaaataaa cagcaaccga tgaagaattt
      481 aggaggagtt gaattcacat ccttttcttc ataattagtt gctgtctatc taagaagaag
      541 tttaaaacga tgagggagat tgcctccctt cgtttatttt gtcgatcctg tttcctaatc
      601 tttcgacact accataataa cactggtaaa tgtctaaaaa ccgccatcat tccgccaaaa
      661 aaccgccaaa ttatttataa gcaattattt ttccgtgttt atatgcttct gcaaactcta
      721 ttagagcttc cgacttcatc cgttgtatac ttctttctga ataacccact tcacggctaa
      781 ttctgtagtt tgagaagcta tctggcacac agaagctgta gtagagtatc tgacgactaa
      841 tcagactaag agccatcaaa gccgctagaa tcgcatctct ctccgtttct atatccatca
      901 tctgaataat cgcgtcttct gccttattgc catgcttcgg tgccttcggc atatccgtta
      961 taatcggcga cttaatatct atcaaagagc gacctgccat ccgctccaaa cgccgaaagt
     1021 tcttcagcac atctctcgca ttacatcttg tctgtttgaa atctacctct cgtaacaatt
     1081 gcatcaagtc aaaccgctcc tttatgtgat ataataaatg tgttggattt attgaatcag
     1141 tcggagcgat ccggcttttt ttatttgctc ttgattactt ccatgtcaac taatctcgct
     1201 acagctaaat aatgcttgtg ttttgcagtc gaacgatcac acttcattgt attttcaata
     1261 cgaatgattg ctgagtttga cagtatcttt ttaacgtatc ctctgaacgg ataaacgaac
     1321 tcttctgctt cacagcggac catgtcaccg actttgaatt ttgatttctt gcgtgtttta
     1381 gggttctttg tcggcatatc tagcattaaa ccgccgatac cgtgactgct agcgtaaaat
     1441 ccgtctttta gtttcatctt tctaccacct cttccactgt tccattcacc aacaatgctg
     1501 cagcatccgc tgttgcttta tcagtgaaaa gcaatcgatg agatggattg cattcaaaac
     1561 tataatttac tccatctttt ccccataaag cacatttagt aagatagttt ccatcggcat
     1621 tctttattgc ccataacggc tctttctcaa cctcgtagcc gttgtatagg ctcaatagtg
     1681 tttcatatga ttgtcttgtt gcccaagcga gtaattcttc tccttgctct tgcgtcacta
     1741 tgccatctcg tacaaaccaa tcacagaaat aatatgaatc accatcttta gatttgatta
     1801 aatacgctat tttttcagct tttgtaaaag ggtccgtaga tttctcaagc caaccggcca
     1861 cgagttgtgg aataactggt ttctgcggtt cgtctatttt tttcattaac gtaatacctg
     1921 aatttatact ctcgttatag caagcagcta ttgcatcatt tcctatcgct ttaatacttt
     1981 ctaacttttt gattgcttcc tgtttattca tcgctgttcc tccaataact cgctattctc
     2041 gtatatattt ccggtgactt cgtacgtgta ttcttcaaac agctctgtgt taaaaatttg
     2101 atactctaag tcttcatcaa cagtggcaca aactaatccg gaataatcta ttgaattttt
     2161 gacaatacag actttattat ctaagtaatc gaagccattt cgcacgctga ctaatactat
     2221 atccccttca aatatatcca caccattctt atctttcatt cctgtggatt gcatgaggtc
     2281 gatgtttcgc acgtgatatg atttcaattc ggtaggattt atccaatatt caataaagtt
     2341 tgttttgccg tttttagtaa aatgcaagac ggctacatct ctcattacgt tctttcgttt
     2401 atcccacgct ctaaacttcg gtatcattcg ctgtcctcct cgtatttttc aatcaattcc
     2461 attacttttt tcactatttc aaactcaacc gcttttgatt cttcgaaatc atgaacaatt
     2521 tctggaaaca gtacatcatc aactacccac aaaataatct ggcgctttcc accaaaattt
     2581 atgattagat ggtccgattc cacagatagt gttgctcctg attcgatatc atataaatcc
     2641 atgctgaatt gaacgagttt ttttatcatt tgctgtcctc ctcaaatact cttctaatat
     2701 ttctttatac ttctctacaa atttgaaacg atcttgatga agtttcttgc tccaatttgt
     2761 ttgccgatcc agctcacgca tctgatcgaa ccctttttga atttcgttgt aataaaattc
     2821 aatgtttgct gctgctttcc aatgcctgct acttcgcact cctgctcctg tttcagccat
     2881 ttctaactta actaattcag ctcgttcttt tgatttttta tctttctgaa tcttcatcat
     2941 gattttcttg aggatgatgt cactgtattg tgtaatgaga tccattatct ctcctccaca
     3001 tacctaaact gtcgtccttt tgaatcaatc cataagctcc tagctctatc ccagataatg
     3061 tttttgctta atccagtaat ttcagataac tgttcagcag tacctgttac taaaattcgg
     3121 tcatcatgcc agattgcaat ttttctcggc gttctccgtt tgggcttttc agtccacatt
     3181 gatttaccga gcttttggac ttctgcaact atttctttgt cttcttgcca agattctgac
     3241 ttggttaatt cagcaattct tttcattgct gctttcttat ccacgctcat tcctccaatc
     3301 gatggatttc ccttcttaaa ttctctatgt gcaaatcgat tgcctttctc gccgtttcat
     3361 tgaccatcac tgcctttgtt cgttccagat cgtcaatctc acgctgaagg cttcgaatac
     3421 gcatttgaat cacttcttct gttgtcatga tgattcctcc acgtatctaa acgttctctt
     3481 cttagcgtct gtgtagccac accttgctct tttcctcacg attttctcgt gcaagcctgt
     3541 gagacttgct aactgttcag ctgtccctat cactagaagt ttgtcaccgt gccaaattgc
     3601 gatttttcgt ggtcttggct tgttgctctt gtctgcccac atcgctcttc caagcctcat
     3661 cacttctgaa gcagcttctt tgtcattttg ccaatcttct gaataagtca attcgataat
     3721 tcgctgcatt gccgctttct tatccatccc gacattctcc tttcagtaat ttgagtactt
     3781 gatcaagtgc gctctcacgt ccgccatgga acgtgttgag ccacttgtct tcgtacgata
     3841 cactttgtct taaagcttct tgatgcatta gttcaatctg tgctgtaaat gtctttaggt
     3901 ccatctgatt acacctgctc aagttcatta agatgttttt gcaagccttt aacacaatca
     3961 acaaatagta atttggtata tgctaaattt cttaattgtg ttgtatcgat ataaagtgcg
     4021 aaatagtatc tgagcttact ccaactcgaa cgatcattct taattccttc aattccagct
     4081 tcttcgagtt gatcatatac gtctctcagg acgtctaatt cctcgccctt tttataattc
     4141 gctatttcat taattagttc tagataatcg atcttcattt ttccacctct tagaatggtg
     4201 cttttgattg tctattagct cgttctagcg cttttttctt gagataggct tcttggtcga
     4261 ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt gtatccactg cttgcgttct
     4321 taggttcact tttttctttc cttgcccaac ttcgaatagt tgccaaatag tttttataag
     4381 tcttaccaga tgattcacaa tactctgaca gtcgttcgat tcgctcttgg taatcattag
     4441 ggaattctgt tttgagtttc tccatctgct catctgacaa aagaacattt ttatactctc
     4501 cgtatttatg acggacgggc ttagccttcg attttttcga aggcggtaac tctcttatat
     4561 attcttttgt attattaaat gtattattaa tagatgtatt attatctttg actttttcgt
     4621 caataggggt attgcgtttt tcgtcaatag ggtattgatt aattcgtagg tacctattga
     4681 ttatttgatt ggtaccctct ttgtaaatga tttcccgatt caagtatcca aacttaatca
     4741 aatcacttac ccatcgcgat atggtctctt tattcacacc atataaatct gcaaagtact
     4801 cattgcctgc ccaacaaaag cctctttcat tacacaaggc cgttatctct ccgtataaca
     4861 acttagtatt tggtttaagt cttttgtcgt accttacgtt ggctggtata atcgcataat
     4921 aacttcgatg ttctgtcatt tttaccctcc aatatttaac tttttgattg ttttctggtt
     4981 taatttgatc cctttgattt gatatttatt tttgaaatta atcacaccta ttttgtgctt
     5041 ctctgtgtga tggattctgc agagtgctgc aaatgtgtac tctgcatgat caacttcttt
     5101 gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg tcagctcctg ttttgccaca
     5161 gatgcagcag actctttttg tgatgcattt gtagaagtaa tattcttgat tcgcaggtaa
     5221 aatctcatag ccttctttga aaggaatatg atgttcaaag atgaaatcta agatgatatt
     5281 tgctaagaca ttggcatcac tcacagttgt attcgattcg tctttcaggc ttattttgcg
     5341 ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc cagaagtccg ttggcatgcc
     5401 tgtatcgata aaaatatcgc ctatcagcgc atagatgaag tttcgttgct gtacggtgaa
     5461 acgtctagga tcaataaaac gaatttcaat gactcgatca ccatcgtagc cgtcatacat
     5521 cgtctttagt cgatcaatgt tcacttcctc attgatggtt gcgcttatgt cttttccttt
     5581 gaactttttc agaaccgctg aatatgaatc gattaatggt ttaaacactc atatcacttc
     5641 ttatctaatt cttttctctt agctgctatt gctcgctcca tcaaggcaca ttgctcatag
     5701 cttaactgtt caatagtttc aacgttatca gctaagagcc ctaatttatc tgtctgctca
     5761 ttaacatatt cgattaaggt tttggtcata tctttaccca tctgctcatt gaaagcttct
     5821 agaatcgtct ctagcatgtt taatttcttt gtatcgattc taggtggtgt tggaatatct
     5881 tccccttgaa atacatataa tcccagtccg tgtagagcca atgctttcac aaagcatcgc
     5941 ttcaatgagt tattgatttg cattgcattt ggtttaacaa ctggttggtt tcgataatct
     6001 aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg ttactgagac agatacataa
     6061 gtcccagttt catccataag aaaaggttta tattcctcaa caagaaagtc ttgatgagtt
     6121 ccagaaacaa ccctgtagtg tttgtactca ttaatagtta ccgttgcctg tggatcattc
     6181 tttttcataa tctcccacgc gtgagcccaa gataaataat caaaatttcc ttttttcttg
     6241 agaattttat ttaacttacg actaaaaagt ttttcaaagt tcgttgtccc tttgatttca
     6301 ctcatcaaat tctgcctcca tttcagcaat gtatttctta cctggtccgt aataagagat
     6361 atcaatcaag ttatctctgt cgtactcttc tagcgcatca atcaagccat cttcgatgac
     6421 gtaaatatat tcaggtttat tcgaatgctt cgatagatgg ataagataaa catgatccca
     6481 aatagttaca tggttgccta aatcatcttg atcccaagct agttcttcat tcgtcaaaag
     6541 atttcgtctg atttttcgac cacttgtttc ctcaattttc ggcttgcccc aatcaggatc
     6601 aatcaaatat tgatctagag tggaaagttc tttttccata tgctaaaatc tccttatgat
     6661 gtgttttctt tgtgactctt tgcttgccgg cggagtcact tttttatttg ttgccatgct
     6721 ttttgcttgt caatatgttg ttggcttagg atgattggtt tatagtattt ccaccagcaa
     6781 ttagcaattg ccgtccctat tcttagcgct tcagctctat tcattgtcat caccaaaaag
     6841 tctctgttgt ctgttcagtt gatcaatttc catacggatc gcagtttctg gcaaccacat
     6901 ttcaataaat gaaacagcat catcgaatct cttacgaggt aactcgccat atcttgggat
     6961 tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat acttttttgc tgatttcttc
     7021 ataagctcgg ctttcttttc cccctagaac tcccataact ttcatatttc ctttttgctt
     7081 aatttcaaac tcttgttgtc cgctaattcg catagtatct ttaagcatgg aaacatcttt
     7141 tttaacatct ttcatttctt ctaattgata gatcatcata tcttcaattg tttgaggaac
     7201 agtattcttg cgaataacat cttccatttc gttgaatgct tcaatgtatt tttgtttgaa
     7261 gtagatagct ttctttcctg taaaccccat agccagcaag aaaaatccat ctctactaat
     7321 gaagaaaact cgtcgatttc tgccatatga atctggttca ttaccttcta caaacatctg
     7381 tccaaaattg gacacatctt tttttagtgc atcaatatct cttaaaacat gttgatgttt
     7441 tttctcgaag ctttctgcca cttgtaagct cgtagtcaca gcttctttat ttttcaaaat
     7501 tactaattct tgcattattt cttctctcct ttttggtata atttagttaa aaactggtgg
     7561 tgtttatttt gaattttttt cacttaacaa tttttcaatg gatgtcaata ataagtttta
     7621 tattgagtac gtttttaaca gggataaaaa taaaaggaat gcgccctcaa ctagaagttg
     7681 aacttaatgc ttcttacttc gctgcagata tgatttatac aaaagtaatc atttctaact
     7741 attcaacaga accagctatg ttagttaatt tagaactttc atccgctaac ttaaaccgca
     7801 gatggtcagc tactccattt aagaaattaa ttgctaaagg aggatcagtc gatgataata
     7861 gaatatactc tgaagctgta ccgctaaata ttcctcctaa aagtgctata tcttgttacc
     7921 ttgcatttga agtagggaaa attaatttta ataaactcct taatcaccaa acaaaaatga
     7981 tatttactct aaatagaacc caaatccaca aaattgttga tatcaaaaat acaaacttcc
     8041 caatagagaa attagtgaaa gaattaaact gagtaatagt aatatacatt ctagtttctc
     8101 ttttctattc atttttagtc agccccctcg gttggctttt tcgctctgta ctcagcttca
     8161 gcttcatcaa gccccataaa aatccaaacc atgtaaacaa tcgttcctat caacgcttgc
     8221 ctgcttcccc aaagtcctaa agcgtagatg attagcggtg cgctgaacac tagcgctcta
     8281 ttaaattttc ccatccgctt gcctccttaa gatttccgaa aaatttgttt ctaaaaattc
     8341 aagtgtttta cttcttaaaa atagatatgt gtctcttcct tcaactggat aatagacaaa
     8401 tccatttttg tttttctcga tatcgataat atttctatat cttgggttct ttaaaactct
     8461 agaagtaaac caatcatatt ttctgttaat ccgttctagc acttctggca acgtcatcca
     8521 tctaccagta tcatcagctt tttttaactc ctcataatcc acttgggaga taattacata
     8581 gccttctgga attgggattt ttgcttctag atattgcatt agctgttcct ccctttacaa
     8641 ttcgtacata gtgataatcg aatctataat tctgtttgct tctgcagaag tctttttacc
     8701 gtttaaaatt aaagataagt agcttttgct aattccaaat ctttcagcaa gcatggtgta
     8761 agttaagaac tttgaacttt cgacatattc tttgattttt tctctatctc gttgagtgat
     8821 ttctgcaatg tcagtcatac taaaactcct ttctaaccaa tttcctctaa atccatttga
     8881 gggtaatatc cttctttttt tagtaattcg tagataaata gacgcccttt ctgtgtccat
     8941 ttggtattca ttacaatttt agtgccacca tcggctttcg ggatctcagt tgtatgagat
     9001 tttgtgtatc cttggttcat gtgttttttg cacaataacc attggttacc gacttttttc
     9061 tgaataccta gtttatgaag taatttattc atctgttgtg gagacatccc ataatctgct
     9121 gcaatctgac taattgttac tgaatctgta gaagataata tgctatctaa ataggagatt
     9181 ttaggttcgt actctgcaat cttttgttct gcgattaatc ttccagttct ttcttctttt
     9241 agttgagttg ctaattgaat gattgtatct ggattaagca aagcttcttc tactttttct
     9301 ggagttagat aacctccatg ttttctaatt gctggcaaca cttcacttgt tacccaacgt
     9361 ttaaattttt tggcagaggg aagttttgat tttaagatta aactgtacaa accagattcg
     9421 ttgatgattg tcatctctct tgattgacct gaggtcgtga ttcgcgaccc catcttatct
     9481 tccaaatcta catgccgcga caaagcatct ttagtgtttg aataacccaa aacactcgca
     9541 acgtctttgc ctacaaaata tggttcatca tttactaaaa ttgtccgaac ttcgttttgt
     9601 tcgaaattaa aaatttgcgg tgtgttcata tttctcattc ctttctttta tattcgtaaa
     9661 caaatttaac aacttttttc taaattcaat tgacagtatc tgagtaatac tctatactaa
     9721 gagcataatt aaataagaca taaaaacatt gattttaaag ctttcttggc ggtctgcatt
     9781 tacatatcaa tagtgttttt tgttgtcttt ttagttgtta aatctgttta caagatatag
     9841 tatagctcat tactcagaat tagtcaaatt atttctgcgt atttttctaa actttttttg
     9901 taaacattta gaaaggttgt tttatcgatg aatacctatg aaataataaa agacttggcg
     9961 aagaggaaaa agatatctat acggcagtta gaaattaatt ttggatattc taacggttat
    10021 ctagcaaagt ggaaaaataa tactcctaat tcagatgaat taagtcggat tgctgactat
    10081 ttcgatgtct ctgtagacta ccttctaggt agagaagaaa gagaaacccc taaacatgtg
    10141 gatttatcag aagacgatac tgttttttct tttgatggaa aagaaatatc taaggagaca
    10201 atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg
    10261 tttgtatgta tttgaaattg aaagaaatgt tgagcgaata caatttgaaa gtagtttata
    10321 tggagatgaa agagcctggt ttctactatc caaaacccag aatcattttt ttgaatgaaa
    10381 atctatacgg tgaaactgca gaagcctttc atttatctca tgaacttgca catttcagcg
    10441 cctctcattt tgaattttca gttttgtacg atacttctac aacttttcac tcaaaatttg
    10501 aaacagaagc agataaaata gctattctaa ttttattaaa tatctatatt gagaacgaac
    10561 tgactgatga atctcagttt aatctcgaaa aatttatgga gtattattct attcagaata
    10621 agctcagata cacttgttat gctgtctgcc aatgctattt taagaaaaaa tattcttacg
    10681 caaggcaata tgtatgaata catcaagaat tattaaaaaa accgatttca gcaaaacatt
    10741 aaaaaagccc gtgctgcaac acgggctctt tcctcatata tgagcttcta caaaaaaaat
    10801 catatcacag aaatgaggaa gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa
    10861 aaaccttgat taaagtaact gatgattcta tttcaattat tagaaaagga tttataaatt
    10921 tagttaatca aggtattaaa ggagaaaaaa ctatcccctt taaaaatata tcagccgtac
    10981 aattaaaaaa accaggtatg agtaatggtt atatacaatt taccttgcta ggcggaaacg
    11041 aaagtcgcgg aggtatatta gcggccacga aagatgaaaa caccgttatg tttacaaaaa
    11101 aatattggaa tgaaatggaa aatttaaaaa aatatataga gaagca
//
LOCUS       DAJSVP010000080        11134 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSVP010000080 DAJSVP010000000
VERSION     DAJSVP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.084"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            135..308
                     /locus_tag="ON449_002131"
     CDS             135..308
                     /locus_tag="ON449_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934700.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            948..1421
                     /locus_tag="ON449_002132"
     CDS             948..1421
                     /locus_tag="ON449_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3934701.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="ON449_002133"
     CDS             1430..1657
                     /locus_tag="ON449_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934702.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="ON449_002134"
     CDS             2293..2664
                     /locus_tag="ON449_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934703.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="ON449_002135"
     CDS             complement(2920..3162)
                     /locus_tag="ON449_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3934704.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="ON449_002136"
     CDS             complement(3194..4096)
                     /locus_tag="ON449_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3934705.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="ON449_002137"
     CDS             complement(4109..4297)
                     /locus_tag="ON449_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3934706.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="ON449_002138"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="ON449_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3934707.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="ON449_002139"
     CDS             complement(4912..5805)
                     /locus_tag="ON449_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3934708.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6806)
                     /locus_tag="ON449_002140"
     CDS             complement(5883..6806)
                     /locus_tag="ON449_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3934709.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6855..7205)
                     /locus_tag="ON449_002141"
     CDS             complement(6855..7205)
                     /locus_tag="ON449_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3934710.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="ON449_002142"
     CDS             complement(7238..8140)
                     /locus_tag="ON449_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3934711.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="ON449_002143"
     CDS             complement(8133..8990)
                     /locus_tag="ON449_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3934712.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9335..10144
                     /locus_tag="ON449_002144"
     CDS             9335..10144
                     /locus_tag="ON449_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3934713.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10184..10681
                     /locus_tag="ON449_002145"
     CDS             10184..10681
                     /locus_tag="ON449_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3934714.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3257 a         2088 c         1871 g         3918 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccaccatat agccaccttt ttcttaagaa tgttttttga aaaaattcat
     9241 aatgacaaag caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga
     9301 aaatagatat gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga
     9361 cggtaccttg cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa
     9421 atttgatgaa aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa
     9481 agatattttt tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat
     9541 tgctgaaatg aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga
     9601 tacactgaga aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg
     9661 agaaacccaa gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta
     9721 ttatgaacat ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga
     9781 tccttctctt agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa
     9841 gctacaaaga gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt
     9901 agaaatcacg cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct
     9961 tggaatcgaa tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt
    10021 gaattacagc gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc
    10081 aaaccaagta acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta
    10141 ttaaaaagaa taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga
    10201 tatgcaaaat catattcttg atccaaacag cgaattttat attccagagt cagatcaatt
    10261 ggcagaacgt attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt
    10321 cacacgggat ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt
    10381 gatccctctt ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat
    10441 tccacctgaa actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa
    10501 agagatcgaa gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct
    10561 gcagagtgct tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa
    10621 acacggagaa tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata
    10681 aaaatatcta tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt
    10741 ttagtcctac gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac
    10801 ggcaggctca cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt
    10861 aacaaacggc aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa
    10921 acgctgattt cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa
    10981 gaacagccaa gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc
    11041 agggcagctt tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg
    11101 ttatgagccg tctgctctaa ccaactgagc taaa
//
LOCUS       DAJSVP010000081        11084 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSVP010000081 DAJSVP010000000
VERSION     DAJSVP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.085"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            173..1129
                     /locus_tag="ON449_002146"
     CDS             173..1129
                     /locus_tag="ON449_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3934715.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1219..2322
                     /locus_tag="ON449_002147"
     CDS             1219..2322
                     /locus_tag="ON449_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3934716.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2335..3288
                     /gene="menA"
                     /locus_tag="ON449_002148"
     CDS             2335..3288
                     /gene="menA"
                     /locus_tag="ON449_002148"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3934717.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4102..5448)
                     /gene="gor"
                     /locus_tag="ON449_002149"
     CDS             complement(4102..5448)
                     /gene="gor"
                     /locus_tag="ON449_002149"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3934718.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5582..6835)
                     /locus_tag="ON449_002150"
     CDS             complement(5582..6835)
                     /locus_tag="ON449_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3934719.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6841..7764)
                     /locus_tag="ON449_002151"
     CDS             complement(6841..7764)
                     /locus_tag="ON449_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3934720.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7985..10477)
                     /locus_tag="ON449_002152"
     CDS             complement(7985..10477)
                     /locus_tag="ON449_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3934721.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10484..10951)
                     /locus_tag="ON449_002153"
     CDS             complement(10484..10951)
                     /locus_tag="ON449_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3934722.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3266 a         2264 c         1942 g         3612 t
ORIGIN      
        1 gtctaactcc ttttggattt ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg
       61 ctgaaagaaa acgtttgaaa tattacatgg gttatttatc aaaaatagtt gacttatagg
      121 ctaaaaagat tagaatgtat aatgaatcac aaagaaaaat tgtggactag aaatggaatg
      181 gtggggtatt aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat tagcattttc
      241 agctctagtg ttaggcgctt gcggcgcaga tgacaacgga agctcaaact cttcttctga
      301 atcatctaca gcacaatctt cgactgcaaa aagcacaaca gaatcatctg caaaagttgt
      361 agctggcgga gatttgcaag acggcacata taaattagaa gaaaaaaatt attcaaatgg
      421 ttaccgtgca gtctttgaaa tggttgtaaa agacggcaaa atcactgaat caaaatatga
      481 taacgtcaat gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca aaaacatgga
      541 agcaaaatct ggcacaaacc cagaaaaata cattcctgag ttgaatgacc aattcttaaa
      601 agcacaaagc gcaagcggtg tggaagtcgt aactggagct actcactcat ctgaaagctt
      661 ccaaaactac gcacaacaat tgatccaagc tgctcaagct ggtaacacag atacaatcga
      721 aatcgacaat ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa agaactactc
      781 taacggctac cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa
      841 ctttgattat atcgacaaag acggtaaatc aaaacaagat gatacagaat ataatgaaaa
      901 tatgaaagca aaaagcggta cagaaccaaa aacttacatc cctactctga acgacgaatt
      961 cgtcaaagca atgggcgaag aagatggctc acctgctaat gtagaagttg ttacaggtgc
     1021 tacacacagc tcacattcat tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa
     1081 aggcgacact caaacaatcg aagtagataa tatcgtaaca gaaaaataat cacataatca
     1141 tactaataaa agttggaggg tgggacgata gccagattag gcagtcgtct cacctttcaa
     1201 aatgaagaga ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac actgggcatc
     1261 tcttttatgg tgctaactgg ctgcagcaac tcagcaaata aaacaaccac tgaaagttca
     1321 gcagctacca agatattgaa agatccatat tctgatgaac agttcttgtt agggacttac
     1381 gttcgtatcc gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga
     1441 gtaaaagaac ttggtgataa aatcacgatc aatcaaaaag gttctgagat cgatgaagtg
     1501 aatgagcaag ctgggatcaa accagtaaaa gtttctgatg acgtctacac gttagtcaag
     1561 cgggcctacg aatatagcca agattctcaa ggcggattcg atatggcgat tggtgcaatc
     1621 acacaattat ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga agaaatcgat
     1681 caggcattga aattagtgga ttatcataag atcgaactaa atgacaaaga aaaaactgtt
     1741 tatctaaaag aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg
     1801 gacgaagtag tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc
     1861 ggtaatgtct atgtcttagg ccacagccca agaggcgaaa accaagactg gacagtcggc
     1921 atccaagatc cgaacatggc tcgcggatct gttttaggtt ctattaaaga acggaacaaa
     1981 acattagtta cctctggaat ttacgagcgt tatttagaag tagatggcaa aaaataccat
     2041 catttgtttg atccaaaaac gggttatccg ttcgacaatg atatcgcaag tgtcacgatc
     2101 ataacagata aatcaattga tggtgacggg ttgtctacag cagtcttttc aatgggtgtg
     2161 aaaaaaggac tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg
     2221 acaaaagacg ataaagtata tgtgaccgat ccaatcaaag acaccttcaa attatcggag
     2281 gactcccatt acacgatggg tgaccgttct gaactaaaat aaggagtacg aaccatgaca
     2341 ctaaaaatct ttttagaagt agttgaaata cgaacaaagg tggctagtgt atttcctttt
     2401 gcggtagggg tattattctc tatcgctttt tttcatgaga tacattggct aaacactttt
     2461 ttatttttta ttggaatgct agtttttgat ttagcgacta ccgctatcaa taattacatg
     2521 gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat tggccgcaga
     2581 aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt tgtttttgtt
     2641 atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg atttatctgt
     2701 tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat gcctttaggg
     2761 gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat
     2821 acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca atttgctttg
     2881 gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt ggtgtttacg
     2941 attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat tgaaaatcat
     3001 cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt ccagctattg
     3061 atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta tgaatggccg
     3121 atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca attcaagcaa
     3181 acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt attcaatgcc
     3241 agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca gcatgaagag
     3301 gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa tagctcctta
     3361 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttatcag
     3421 gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt
     3481 ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc
     3541 agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc
     3601 ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt
     3661 cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg
     3721 attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt
     3781 aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg
     3841 ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg
     3901 cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt
     3961 ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa
     4021 aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct
     4081 gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg
     4141 gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa
     4201 accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc
     4261 ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta
     4321 cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc
     4381 ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac
     4441 aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct
     4501 accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa
     4561 aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa
     4621 ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa
     4681 aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt
     4741 gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta
     4801 catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg
     4861 atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat
     4921 gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt
     4981 tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc
     5041 gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat
     5101 aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc
     5161 accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt
     5221 ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt
     5281 acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa
     5341 ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc
     5401 aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct
     5461 tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt
     5521 aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc
     5581 cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta
     5641 aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt
     5701 caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag
     5761 aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt
     5821 caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag
     5881 ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt
     5941 cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg
     6001 ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct
     6061 tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca
     6121 ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt
     6181 gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa catccgccac
     6241 ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac
     6301 acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca
     6361 cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga
     6421 attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg
     6481 gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac
     6541 cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga
     6601 ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag
     6661 ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa
     6721 tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg
     6781 gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc
     6841 ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg
     6901 atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc
     6961 taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg
     7021 agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc
     7081 attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat
     7141 agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt
     7201 ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg
     7261 gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc
     7321 cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc
     7381 ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat
     7441 acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac
     7501 atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg
     7561 atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac
     7621 caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc
     7681 accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc
     7741 tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata
     7801 gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac
     7861 aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa
     7921 atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa
     7981 gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac
     8041 cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct
     8101 cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt
     8161 caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa
     8221 tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga
     8281 tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag
     8341 gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag
     8401 taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat
     8461 ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta
     8521 aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca
     8581 cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac
     8641 cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt
     8701 ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg
     8761 aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac
     8821 caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg
     8881 ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca
     8941 agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa
     9001 ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa
     9061 ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag
     9121 cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt
     9181 ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg
     9241 ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa
     9301 cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt
     9361 catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta
     9421 cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt
     9481 catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga
     9541 tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat
     9601 caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat
     9661 cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca
     9721 ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt
     9781 cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc
     9841 gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca
     9901 gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc
     9961 cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc
    10021 cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc
    10081 tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca
    10141 tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta
    10201 aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac
    10261 ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat
    10321 ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa
    10381 catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta
    10441 atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat
    10501 aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat
    10561 tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa
    10621 ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat
    10681 catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg
    10741 caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg
    10801 taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca
    10861 tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt
    10921 caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac
    10981 cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc
    11041 tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAJSVP010000082        10792 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSVP010000082 DAJSVP010000000
VERSION     DAJSVP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.086"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            168..1040
                     /locus_tag="ON449_002154"
     CDS             168..1040
                     /locus_tag="ON449_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3934723.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /locus_tag="ON449_002155"
     CDS             1037..2995
                     /locus_tag="ON449_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3934724.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3327..4178)
                     /locus_tag="ON449_002156"
     CDS             complement(3327..4178)
                     /locus_tag="ON449_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3934725.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4301..4768
                     /locus_tag="ON449_002157"
     CDS             4301..4768
                     /locus_tag="ON449_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3934726.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5031..5513)
                     /locus_tag="ON449_002158"
     CDS             complement(5031..5513)
                     /locus_tag="ON449_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3934727.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5613..6476)
                     /locus_tag="ON449_002159"
     CDS             complement(5613..6476)
                     /locus_tag="ON449_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3934728.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7074..7949
                     /locus_tag="ON449_002160"
     CDS             7074..7949
                     /locus_tag="ON449_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934729.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8094..8246)
                     /locus_tag="ON449_002161"
                     /pseudo
     CDS             complement(8094..8246)
                     /locus_tag="ON449_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8514..9845)
                     /locus_tag="ON449_002162"
     CDS             complement(8514..9845)
                     /locus_tag="ON449_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934730.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(9994..10707)
                     /locus_tag="ON449_002163"
     CDS             complement(9994..10707)
                     /locus_tag="ON449_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3934731.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT         3408 a         2145 c         1827 g         3412 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
     3241 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
     3301 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
     3361 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
     3421 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
     3481 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
     3541 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
     3601 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
     3661 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
     3721 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
     3781 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
     3841 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
     3901 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
     3961 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
     4021 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
     4081 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
     4141 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
     4201 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
     4261 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     4321 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     4381 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     4441 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     4501 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     4561 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     4621 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     4681 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     4741 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     4801 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     4861 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     4921 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     4981 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     5041 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     5101 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     5161 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     5221 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     5281 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     5341 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     5401 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     5461 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     5521 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     5581 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     5641 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     5701 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     5761 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     5821 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     5881 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     5941 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     6001 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     6061 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     6121 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     6181 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     6241 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     6301 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     6361 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     6421 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     6481 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     6541 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     6601 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     6661 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     6721 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     6781 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     6841 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     6901 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     6961 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     7021 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     7081 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     7141 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     7201 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     7261 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     7321 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     7381 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     7441 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     7501 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     7561 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     7621 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     7681 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     7741 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     7801 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     7861 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     7921 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     7981 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     8041 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     8101 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     8161 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     8221 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     8281 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     8341 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     8401 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     8461 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     8521 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     8581 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     8641 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     8701 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     8761 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     8821 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     8881 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     8941 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     9001 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     9061 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     9121 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     9181 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     9241 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     9301 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     9361 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     9421 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     9481 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     9541 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     9601 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     9661 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     9721 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     9781 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     9841 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     9901 atcacttatc aaaaaaagta aatactttta attaaatata tatatctttt ttagtataaa
     9961 aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa
    10021 aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag
    10081 agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc
    10141 gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc
    10201 cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg
    10261 tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg
    10321 gcttcgtaca atattatata catagtcttt tacactgata cttaatttat cagctacttt
    10381 tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac
    10441 cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac
    10501 gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac
    10561 gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg
    10621 aagtttctca ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat
    10681 ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc
    10741 tatactttaa cataaataaa ccgataaaat ccacatcaaa attatacaca ca
//
LOCUS       DAJSVP010000083        10719 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSVP010000083 DAJSVP010000000
VERSION     DAJSVP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.087"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1397..2062)
                     /locus_tag="ON449_002164"
     CDS             complement(1397..2062)
                     /locus_tag="ON449_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934732.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2242..4224
                     /locus_tag="ON449_002165"
     CDS             2242..4224
                     /locus_tag="ON449_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3934733.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4228..4866
                     /locus_tag="ON449_002166"
     CDS             4228..4866
                     /locus_tag="ON449_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934734.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4894..5322
                     /locus_tag="ON449_002167"
     CDS             4894..5322
                     /locus_tag="ON449_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934735.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5779..6690
                     /locus_tag="ON449_002168"
     CDS             5779..6690
                     /locus_tag="ON449_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3934736.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7144..7458
                     /locus_tag="ON449_002169"
     CDS             7144..7458
                     /locus_tag="ON449_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3934737.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7474..7860
                     /locus_tag="ON449_002170"
     CDS             7474..7860
                     /locus_tag="ON449_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3934738.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7889..9274
                     /locus_tag="ON449_002171"
     CDS             7889..9274
                     /locus_tag="ON449_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3934739.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9277..9429
                     /locus_tag="ON449_002172"
     CDS             9277..9429
                     /locus_tag="ON449_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3934740.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9452..10657
                     /locus_tag="ON449_002173"
     CDS             9452..10657
                     /locus_tag="ON449_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3934741.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3638 a         1748 c         2076 g         3257 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agatatcatc cataaaatgg actttgtcta cagtctgagg actacctgta ttagtcctct
      181 tttttgacca gattttgacc agtctatttt acattctaat atattcatat gagctaattt
      241 tatgattaaa tggtaagtgt ttccttgttt gttttagttt aaactagtgt gtaatttaaa
      301 aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac ctgataacat
      361 caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt aaagagccga
      421 gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac agtcttggtg
      481 ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa gatgttgata
      541 aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa gctaatttta
      601 agagattctt attagaagac gtttaggaat ataactaaga aaatgattat acaggtaatt
      661 gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag gccagaatca
      721 gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa tattcccact
      781 aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc ccatgctacc
      841 gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt ctgccattac
      901 tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat gataggcgct
      961 tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc gattatcata
     1021 tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat agaaatataa
     1081 aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat ctatattact
     1141 tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg tgaatgtcca
     1201 tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata ttatactata
     1261 agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct caaggctttt
     1321 tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt tttctatctc
     1381 tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt aaggcgtcgt
     1441 catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa gtgagcaggc
     1501 gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc tctttttcta
     1561 cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta catacccaac
     1621 gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt tgtggtttca
     1681 aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata agaaaaagat
     1741 agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag aagggttgtt
     1801 ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt tctattgatt
     1861 cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga tgtaaaagtg
     1921 acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc gttttatcgg
     1981 aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg cggtaagttc
     2041 gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata tagatttatt
     2101 ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta tgattttatc
     2161 aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac agttttactc
     2221 acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa taaacaggta
     2281 ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat tcgattgcca
     2341 caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga aaatgtctca
     2401 tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga agtagataca
     2461 tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca aggggatgtc
     2521 aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac gtccattgaa
     2581 gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag agaaaaagaa
     2641 gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt tcacactgat
     2701 tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt tatttcaaat
     2761 acagacgtat ataattatcg agtgtattta actattggat tgattctttt attccttttg
     2821 ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa taatgacttt
     2881 tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat tacattcata
     2941 attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt tctttatttg
     3001 ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat ttctctttta
     3061 ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata cattcttttt
     3121 acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt agcaactatc
     3181 gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat cgctggtgag
     3241 gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt tatttattcc
     3301 aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa aaacggaagt
     3361 atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg cagagggata
     3421 aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg aaggttgatc
     3481 caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata tagacataca
     3541 gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag tgaaaaagaa
     3601 ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc ttctatcccc
     3661 tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat gtatttggaa
     3721 agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg ctctttgtcg
     3781 aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt gcagttatca
     3841 tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc atttcattac
     3901 ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta tgtcatgtta
     3961 tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag tggttattcg
     4021 ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg aacgacaagt
     4081 gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat ttttttcttt
     4141 agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa gaaaagtcag
     4201 ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa aggtgcgaaa
     4261 aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga tgctggggat
     4321 cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa tatattagga
     4381 ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac gaaacaatta
     4441 actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt tcaagatttt
     4501 gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa gttttcgaat
     4561 ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt aaatataaac
     4621 aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag aatcgcctgt
     4681 gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac tgggtcattg
     4741 gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc ccaaggtacg
     4801 ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa gaagatcatg
     4861 ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat tatttgtgca
     4921 ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc aaattcagtt
     4981 cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga tgctaaaggt
     5041 aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc aattaatcat
     5101 agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac aggtcattct
     5161 aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg aaactctatt
     5221 cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg aaatagaact
     5281 gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa aagtttatat
     5341 aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag ttttaatata
     5401 ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat gtctggaaaa
     5461 tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga
     5521 taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga cttgaaaaac
     5581 ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta ttctgctgag
     5641 cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca gattagtaaa
     5701 ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa
     5761 aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt actaggcact
     5821 ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta taaaggcggt
     5881 acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc agattccgct
     5941 gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga cgtatcaacc
     6001 aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc atttgattca
     6061 caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa acaaagtcta
     6121 gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt aaaaacggct
     6181 tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag tgaagatgat
     6241 gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt agccaaagac
     6301 aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga ttccactact
     6361 acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg tcaagtttct
     6421 gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt ggttaaaatg
     6481 gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt gaaagaaatt
     6541 gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg cgaagtattg
     6601 aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag tacattcact
     6661 tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat gaactattta
     6721 aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag tagtttaatt
     6781 actacttgat ttagttcttt ttctacaaaa aatagcataa aaatctagtt atccgcataa
     6841 aaactggact tatcacactt tatcaaggtc aaaaccactc aatttactac taatttacta
     6901 cttatgaatg agctttgata cgacgattta tccttgaaaa gtgaagatat aaagatactt
     6961 ccaataaaat ttgaatattt aataggtaga cacttcaaaa aatgaggtgt ctattttttt
     7021 acccgatttt gaaaggaagt gaacttatga aaacaaaaaa tcaagaatca aaaggtcgtt
     7081 ccccactctt taagaccatc aaacattcat tcagccaata aaaaagaaag gataggtaaa
     7141 aatatggaac ttaaatttgt gattcccaac atggaaaaaa cattcggcaa tttagaattt
     7201 gctggcgagg ataaagtcgt tcagcgaaga atcaacggac ggctaactgt cttatcaaga
     7261 agctataatc tctattctga tgttcaaaga gcagatgata ttgtggtggt gcttcctgct
     7321 gaagctggcg aaaaacattt cggctttgag gaacgtgtga agttagtcaa tccacgtatt
     7381 accgcagagg gctacaaaat cggcactcgt ggttttacaa attacctttt acatgctgac
     7441 gacatgataa aagaataaag aaagagagga aaaatgatga gattagcaaa tggcattgta
     7501 ttagataaag acacgacttt tggagaattg aaattctctg ctctacgtcg tgaagtgaga
     7561 atccaaaatg aagacgggtc ggtttcagat gaaatcaagg aacgtaccta tgacttaaaa
     7621 tccaaaggac aaggacgcat gattcaagta agtattcctg ccagcgtgcc tttgaaagag
     7681 tttgattata acgcacgggt ggaacttatc aatcccattg cggacaccgt tgctactgcc
     7741 acctatcaag gagcagatgt tgactggtat atcaaggcag acgatattgt gctgacaaag
     7801 gattctagtt cattcaaagc tcaaccacaa gcaaagaaag aaccgacaca agacaaatag
     7861 tcgctaggta gaaaggagac tttttcgcat gaaacagcgt ggtaaaagga ttcgcccatc
     7921 tggtaaagat ttagtctttc attttacgat agcgtcactc ctgcctgttt tcctgctggt
     7981 tgtcggactg tttcatgtga agacaatcca gcagatcaac tggcaggatt ttaacctatc
     8041 acaagcagat aagattgaca ttccctattt aattatcagt ttcagtgtcg caattcttat
     8101 ctgcttgctg gtagcgtttg tattcaaacg ggttcgctat gatacggtta aacaacttta
     8161 ccaccgtcaa aaactggcaa agatgatact tgaaaacaag tggtatgaat ctgaacaggt
     8221 caaaacagag ggtttcttta aagatagtgc tggtcgtaca aaggaaaaga taacctactt
     8281 ccctaaaatg tattatcgac ttaaaaatgg cttgatacag atacgggtgg aaatcacgct
     8341 gggaaaatat caagaccaac tcttacactt ggaaaagaaa ttagagagtg gcttgtactg
     8401 tgagctgacg gataaagagt taaaggattc ctatgtggaa tatactttgc tctatgacac
     8461 catagccagt cgtatttcta ttgatgaagt agaagctaaa gatggtaaac ttcgcttaat
     8521 gaaaaacgta tggtgggaat atgataagct ccctcatatg ttgattgctg gtggtacagg
     8581 tggcggtaaa acttacttta tactgacact gattgaagcc ttgcttcata cagattcaaa
     8641 actgtatatt cttgacccga aaaatgctga ccttgcggac ttaggttctg tgatggcaaa
     8701 tgtctactat agaaaagaag acttgctttc ttgcattgaa acattctatg aagaaatgat
     8761 gaaacgtagt gaggaaatga agcagatgaa gaactataag actggcaaaa attatgctta
     8821 cttaggtctc ccggcacact tcttaatctt tgatgaatac gtcgctttca tggaaatgct
     8881 gggaacaaaa gaaaacaccg cagttatgaa taagctgaaa cagattgtca tgttaggtcg
     8941 tcaagctggc ttctttctaa tactggcttg tcaacgtcca gacgcaaaat atttaggcga
     9001 cggaatccgt gatcagttta atttcagagt ggctttaggt cgtatgtctg aaatgggcta
     9061 tggcatgatg tttggcagtg acgtacaaaa ggatttcttc ttaaagcgaa tcaaaggtcg
     9121 tggctatgtt gatgtaggaa caagtgtcat atcagagttt tatactcccc ttgtaccaaa
     9181 aggatatgat ttcttggagg aaattaaaaa gttatccaac agcagacagt ccacgcaggc
     9241 gacgtgcgaa gcggaagtcg caggtgtgga ctgatcttgc tggctggtgt ggcaatagcc
     9301 acgccagcac ttaacccccc gtatctaaca ggggggtaca aatcgacagg aaacagtcaa
     9361 aaaaacatta gaaaatcctt tggttacaag ggatttacaa aatttcagcg tatgtcaaat
     9421 gggctttaaa agttgacata cgcctttttg attggaggga tttttactga atgaacaaac
     9481 ttggttacag catttaaaag aaaaacgctt ggcttatgga ctatctcaaa accgtttagc
     9541 tgttgcgact ggtattacaa ggcagtatct aagcgatatt gaaacaggaa aagtcaagcc
     9601 atcagaggat ttacagcagt ccctttggga agctctggaa cgcttcaatc ccgacgctcc
     9661 ccttgaaatg ctgtttgatt atgtaaggat tcgctttccg acaacagacg tacagcaggt
     9721 ggtcgaaaac atcttacaac tgaaactgtc ctattttctt catgaggact atggtttcta
     9781 ttcttattca gagcattatg ctttaggcga catattcgtc ctttgctccc atgaactgga
     9841 caaaggagtt ctggtggaat tgaaaggtcg tgggtgcaga caatttgaaa gctatcttct
     9901 ggcacaacaa agaagctggt atgagttctt tatggacgtt ttggtggctg gcggtgtgat
     9961 gaaacgcctt gaccttgcca ttaacgataa gacagggatt ttgaatatcc ctgtactcac
    10021 tgaaaagtgc caacaggaag aatgtatctc cgtcttccgc agttttaaaa gctatcgcag
    10081 tggcgaactg gtacgcaaag aggaaaagga atgtatggga aacaccctct atatcggttc
    10141 attacaaagt gaagtttatt tctgtatcta tgaaaaggac tacgagcagt acaagaaaaa
    10201 tgatattccc attgaagacg cagaagtaaa aaaccgtttt gagattcgat tgaaaaatga
    10261 gcgtgcctat tatgcagtcc gtgatttact cgtctatgac aatccagagc ataccgcctt
    10321 taaaattatc aatcggtata tccgttttgt agataaagac gattccaaac ctcgttctga
    10381 ttggaaactg aatgaagaat gggcttggtt tattgggaac aatcgtgaac gattaaaact
    10441 aaccacaaaa ccagagcctt actccttcca aaggacgctg aactggctat ctcatcaagt
    10501 tgccccgacc ttaaaggttg cgattaaact tgatgaaatc aaccagacgc aggttgtaaa
    10561 agacattctc gaccatgcga aactgacaga ccgacacaag cagattttga agcaacagtc
    10621 agtaaaagaa caggacgtga taacaacaaa aaaataactc aaatacaaat tcattgaata
    10681 tagagaggag aacattttta tgaattttgg acaaaacct
//
LOCUS       DAJSVP010000084        10714 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSVP010000084 DAJSVP010000000
VERSION     DAJSVP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.088"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(232..1080)
                     /locus_tag="ON449_002174"
     CDS             complement(232..1080)
                     /locus_tag="ON449_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3934742.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1079..2146
                     /gene="gnd"
                     /locus_tag="ON449_002175"
     CDS             1079..2146
                     /gene="gnd"
                     /locus_tag="ON449_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR3934743.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2148..3149
                     /locus_tag="ON449_002176"
     CDS             2148..3149
                     /locus_tag="ON449_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR3934744.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3146..3430
                     /locus_tag="ON449_002177"
     CDS             3146..3430
                     /locus_tag="ON449_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3934745.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3463..4260
                     /locus_tag="ON449_002178"
     CDS             3463..4260
                     /locus_tag="ON449_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3934746.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4274..5107
                     /locus_tag="ON449_002179"
     CDS             4274..5107
                     /locus_tag="ON449_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934747.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5125..5982
                     /locus_tag="ON449_002180"
     CDS             5125..5982
                     /locus_tag="ON449_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3934748.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6004..6513
                     /locus_tag="ON449_002181"
     CDS             6004..6513
                     /locus_tag="ON449_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934749.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6550..6951
                     /locus_tag="ON449_002182"
     CDS             6550..6951
                     /locus_tag="ON449_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934750.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7063..7416
                     /locus_tag="ON449_002183"
     CDS             7063..7416
                     /locus_tag="ON449_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934751.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7450..7893
                     /locus_tag="ON449_002184"
     CDS             7450..7893
                     /locus_tag="ON449_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3934752.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7910..8995
                     /gene="uxuA"
                     /locus_tag="ON449_002185"
     CDS             7910..8995
                     /gene="uxuA"
                     /locus_tag="ON449_002185"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3934753.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8988..9701
                     /locus_tag="ON449_002186"
     CDS             8988..9701
                     /locus_tag="ON449_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3934754.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9908..10057)
                     /locus_tag="ON449_002187"
     CDS             complement(9908..10057)
                     /locus_tag="ON449_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934755.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10453..10653
                     /locus_tag="ON449_002188"
     CDS             10453..10653
                     /locus_tag="ON449_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3934756.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3581 a         1694 c         2319 g         3120 t
ORIGIN      
        1 aaaaaaaaga aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca
       61 agtaactcga ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc
      121 caccgactat tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt
      181 tggtagtggc tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt
      241 gatttcagaa atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta
      301 aaacatcaaa atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa
      361 agcttcagat cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt
      421 agcaagggaa gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac
      481 ggtttcagca atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca
      541 gtctttttcc gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga
      601 agttgcgata ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc
      661 gccaacaccg aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag
      721 atccttttgt tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac
      781 cttttgcgcc atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc
      841 agatagatca ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc
      901 aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt
      961 gctaatagga ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg
     1021 ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat
     1081 gagaattcct cattttcata ataagattat cattttttcc gttctcatta atgttaaaga
     1141 tacaaggaga gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa
     1201 aatttttttg tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat
     1261 tggtttggga aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt
     1321 acaaggactc gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc
     1381 ttatcaagag atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat
     1441 gcttcctgct ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc
     1501 aggagacatt attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga
     1561 agagtttcag aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg
     1621 agcacgacat caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga
     1681 acaattattc aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag
     1741 cggacacttc ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg
     1801 tgaaggattc caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg
     1861 tgtttggaat catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt
     1921 aaacgaacat cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa
     1981 atggacggtt gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt
     2041 gttcatgagg aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact
     2101 aagaaatggg tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc
     2161 atatagcaat cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat
     2221 taaagtcagt tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac
     2281 tagcggagtt acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac
     2341 gagatttttt tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca
     2401 acaatattga tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt
     2461 tagttgaacg aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac
     2521 gcacgacaca agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg
     2581 tcggtaggac gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg
     2641 gagtaatcga aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca
     2701 aatcagattt atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg
     2761 aacaatcaga tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt
     2821 ctgataaaga agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg
     2881 ctctagttga tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg
     2941 ccacagatgt tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac
     3001 aagtagtgat tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg
     3061 aaaaatgtgt cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc
     3121 agacaaagag caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa
     3181 aacaggccct caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt
     3241 gaaatcatac ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc
     3301 gctacctaaa ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc
     3361 tggagaatgt agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc
     3421 gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat
     3481 cttgcaagaa tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca
     3541 cctcttgctg tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac
     3601 agacaagctg ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat
     3661 ttcattgccg ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa
     3721 caagattttt tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc
     3781 aaagaagaga ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg
     3841 acatcggagt tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga
     3901 ttaggagata aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa
     3961 tatattccgg aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat
     4021 caactggcac tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa
     4081 ttagaactgc cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata
     4141 gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta
     4201 acacagaaat cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga
     4261 aggaggagga cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt
     4321 agccgcggtt atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc
     4381 tttgatgact ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt
     4441 tacagccgct ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc
     4501 tgaaccatct attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc
     4561 agaagcagcg attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt
     4621 tcgttttttc atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa
     4681 tacaaaagct atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt
     4741 cgttccgctt gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa
     4801 agctttagag ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc
     4861 aatcggtatc gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt
     4921 ccttgcttat tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc
     4981 catttttggg attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca
     5041 gcctgccaca agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg
     5101 attttaattt aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct
     5161 gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa
     5221 attcctcact cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc
     5281 ttgaaaaaac tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg
     5341 ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg
     5401 gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg
     5461 atgtataaca cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat
     5521 tcaggaaccg tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt
     5581 cctttaggcg cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta
     5641 tttttcgcaa gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact
     5701 ggtgttcaga cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt
     5761 gcgggaaact atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt
     5821 gttatccagg aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg
     5881 ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg
     5941 actggaatat taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat
     6001 aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg
     6061 gtaacgttat ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca
     6121 aaagataaat ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca
     6181 attgtcatta caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac
     6241 aattgtattt tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc
     6301 cccatggaag aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta
     6361 gtgacgaaat ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa
     6421 caatatggcg taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta
     6481 aaagatgtat tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag
     6541 ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt
     6601 taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat
     6661 atttacagta tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca
     6721 aaaaatattt atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat
     6781 acacccgttc atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa
     6841 acaatctact ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg
     6901 atgatatata acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc
     6961 actgtactag catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct
     7021 agcagacaaa cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt
     7081 acgattgatg atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag
     7141 ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct
     7201 gttggggcag atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg
     7261 ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa
     7321 aagcaagcgc agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg
     7381 caaaatgcca atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg
     7441 agaagactaa tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa
     7501 gatgcggcgg aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct
     7561 ggggaggaca tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca
     7621 gcagaaccca tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc
     7681 ttggcagtca atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg
     7741 aatctgccaa tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa
     7801 gcggtggaac aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg
     7861 gaagaaaaaa aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg
     7921 atttagatgg tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc
     7981 gggaataaat gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat
     8041 ttcagaaatt gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat
     8101 tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat
     8161 tgataactat atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta
     8221 tagcttcaaa ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg
     8281 aagctattct cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta
     8341 tcaattgata cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt
     8401 gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga
     8461 aaatctgact tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat
     8521 ggctatccat ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa
     8581 tcttggcgac ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct
     8641 gtgcacagga tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt
     8701 aggttcgcgc atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt
     8761 caaagaaacg gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc
     8821 gttggtgcaa gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg
     8881 tgaagtggct atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca
     8941 aggtctatat gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca
     9001 agctcttcat ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg
     9061 cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac
     9121 taactggcca aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg
     9181 ttccatatat caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag
     9241 ataagagagt atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag
     9301 gaatccatac aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa
     9361 tggaaaatct atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa
     9421 agccggaacc agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag
     9481 cacttgtatt tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt
     9541 cttttctcat caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga
     9601 aagtagatgt aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc
     9661 aagcaaaacg atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta
     9721 gtcctcttta tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt
     9781 attttgaagt atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta
     9841 ctgttatcta ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt
     9901 ccactcctta tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg
     9961 ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg
    10021 ttactcgata ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata
    10081 cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat
    10141 tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa
    10201 caagaaaaca aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa
    10261 tagaagacaa tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga
    10321 tatattaaat gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa
    10381 actccttttg cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg
    10441 aggaatttat ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc
    10501 tttatcactg gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac
    10561 ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt
    10621 ggccctcaag caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc
    10681 gaaagaaaag acggtttttt tgtacgaaaa aatt
//
LOCUS       DAJSVP010000085        10698 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSVP010000085 DAJSVP010000000
VERSION     DAJSVP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.089"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            153..2213
                     /locus_tag="ON449_002189"
     CDS             153..2213
                     /locus_tag="ON449_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3934757.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="ON449_002190"
     CDS             2296..2865
                     /locus_tag="ON449_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934758.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3040..3846)
                     /locus_tag="ON449_002191"
     CDS             complement(3040..3846)
                     /locus_tag="ON449_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934759.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3984..5498
                     /locus_tag="ON449_002192"
     CDS             3984..5498
                     /locus_tag="ON449_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3934760.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5901..6884)
                     /locus_tag="ON449_002193"
     CDS             complement(5901..6884)
                     /locus_tag="ON449_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3934761.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6889..7536)
                     /locus_tag="ON449_002194"
     CDS             complement(6889..7536)
                     /locus_tag="ON449_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3934762.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7621..8253)
                     /locus_tag="ON449_002195"
     CDS             complement(7621..8253)
                     /locus_tag="ON449_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3934763.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ON449_002196"
     CDS             complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ON449_002196"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3934764.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8817..9554)
                     /locus_tag="ON449_002197"
     CDS             complement(8817..9554)
                     /locus_tag="ON449_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3934765.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9551..10540)
                     /locus_tag="ON449_002198"
     CDS             complement(9551..10540)
                     /locus_tag="ON449_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3934766.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3470 a         2014 c         2119 g         3095 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
     3001 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
     3061 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
     3121 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
     3181 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
     3241 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
     3301 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
     3361 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
     3421 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
     3481 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
     3541 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
     3601 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
     3661 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
     3721 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
     3781 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
     3841 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
     3901 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
     3961 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
     4021 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
     4081 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
     4141 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
     4201 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
     4261 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
     4321 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
     4381 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
     4441 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
     4501 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
     4561 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
     4621 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
     4681 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
     4741 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
     4801 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
     4861 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
     4921 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
     4981 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
     5041 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
     5101 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
     5161 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
     5221 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
     5281 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
     5341 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
     5401 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
     5461 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
     5521 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg
     5581 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
     5641 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     5701 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     5761 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
     5821 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     5881 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
     5941 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
     6001 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
     6061 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
     6121 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
     6181 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
     6241 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
     6301 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
     6361 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
     6421 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
     6481 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
     6541 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
     6601 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
     6661 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
     6721 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
     6781 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
     6841 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
     6901 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
     6961 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
     7021 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
     7081 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
     7141 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
     7201 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
     7261 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
     7321 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
     7381 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
     7441 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
     7501 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
     7561 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
     7621 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
     7681 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
     7741 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
     7801 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
     7861 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
     7921 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
     7981 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
     8041 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
     8101 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
     8161 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
     8221 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
     8281 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
     8341 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
     8401 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
     8461 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
     8521 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
     8581 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
     8641 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
     8701 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
     8761 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
     8821 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
     8881 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
     8941 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
     9001 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
     9061 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
     9121 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
     9181 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
     9241 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
     9301 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
     9361 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
     9421 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
     9481 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
     9541 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
     9601 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
     9661 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
     9721 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
     9781 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
     9841 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
     9901 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
     9961 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact
    10021 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
    10081 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
    10141 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
    10201 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
    10261 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
    10321 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
    10381 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
    10441 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
    10501 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
    10561 aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag
    10621 acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga
    10681 aatattcagc aaaaaaaa
//
LOCUS       DAJSVP010000086        10655 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSVP010000086 DAJSVP010000000
VERSION     DAJSVP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.090"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            34..657
                     /locus_tag="ON449_002199"
     CDS             34..657
                     /locus_tag="ON449_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934767.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="ON449_002200"
     CDS             1106..2155
                     /locus_tag="ON449_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3934768.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ON449_002201"
     CDS             2152..3033
                     /locus_tag="ON449_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934769.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ON449_002202"
     CDS             3036..3806
                     /locus_tag="ON449_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3934770.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ON449_002203"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ON449_002203"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3934771.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ON449_002204"
     CDS             5117..6280
                     /locus_tag="ON449_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3934772.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ON449_002205"
     CDS             6397..6807
                     /locus_tag="ON449_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3934773.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ON449_002206"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ON449_002206"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3934774.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ON449_002207"
     CDS             9380..9664
                     /locus_tag="ON449_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934775.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ON449_002208"
     CDS             9824..10060
                     /locus_tag="ON449_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3934776.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ON449_002209"
     CDS             10057..10455
                     /locus_tag="ON449_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3934777.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3723 a         1772 c         2254 g         2906 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt tttta
//
LOCUS       DAJSVP010000087        10576 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSVP010000087 DAJSVP010000000
VERSION     DAJSVP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.091"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..84
                     /locus_tag="ON449_002210"
     tRNA            12..84
                     /locus_tag="ON449_002210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ON449_002211"
     tRNA            88..160
                     /locus_tag="ON449_002211"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ON449_002212"
     tRNA            168..249
                     /locus_tag="ON449_002212"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ON449_002213"
     tRNA            267..339
                     /locus_tag="ON449_002213"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ON449_002214"
     tRNA            351..422
                     /locus_tag="ON449_002214"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ON449_002215"
     tRNA            439..524
                     /locus_tag="ON449_002215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ON449_002216"
     tRNA            535..608
                     /locus_tag="ON449_002216"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ON449_002217"
     tRNA            618..691
                     /locus_tag="ON449_002217"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ON449_002218"
     tRNA            711..783
                     /locus_tag="ON449_002218"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ON449_002219"
     tRNA            811..884
                     /locus_tag="ON449_002219"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ON449_002220"
     tRNA            898..971
                     /locus_tag="ON449_002220"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ON449_002221"
     tRNA            995..1084
                     /locus_tag="ON449_002221"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ON449_002222"
     tRNA            1097..1170
                     /locus_tag="ON449_002222"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ON449_002223"
     tRNA            1189..1264
                     /locus_tag="ON449_002223"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ON449_002224"
     tRNA            1266..1336
                     /locus_tag="ON449_002224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ON449_002225"
     tRNA            1350..1423
                     /locus_tag="ON449_002225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ON449_002226"
     tRNA            1448..1535
                     /locus_tag="ON449_002226"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ON449_002227"
     tRNA            1557..1628
                     /locus_tag="ON449_002227"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ON449_002228"
     tRNA            1651..1740
                     /locus_tag="ON449_002228"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ON449_002229"
     tRNA            1753..1826
                     /locus_tag="ON449_002229"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ON449_002230"
     tRNA            1845..1917
                     /locus_tag="ON449_002230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ON449_002231"
     tRNA            1921..1993
                     /locus_tag="ON449_002231"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ON449_002232"
     tRNA            1998..2068
                     /locus_tag="ON449_002232"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ON449_002233"
     tRNA            2081..2154
                     /locus_tag="ON449_002233"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ON449_002234"
     tRNA            2166..2237
                     /locus_tag="ON449_002234"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="ON449_002235"
     CDS             2503..3696
                     /locus_tag="ON449_002235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3934778.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ON449_002236"
     CDS             3826..5298
                     /locus_tag="ON449_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3934779.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ON449_002237"
     CDS             5381..6169
                     /locus_tag="ON449_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3934780.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ON449_002238"
     CDS             complement(6236..6904)
                     /locus_tag="ON449_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3934781.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ON449_002239"
     CDS             7019..8731
                     /locus_tag="ON449_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934782.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ON449_002240"
     CDS             8734..10506
                     /locus_tag="ON449_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934783.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3034 a         1926 c         2454 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcaga
//
LOCUS       DAJSVP010000088        10565 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSVP010000088 DAJSVP010000000
VERSION     DAJSVP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.092"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            184..1080
                     /locus_tag="ON449_002241"
     CDS             184..1080
                     /locus_tag="ON449_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3934784.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1230..2171)
                     /locus_tag="ON449_002242"
     CDS             complement(1230..2171)
                     /locus_tag="ON449_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3934785.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2297..3418
                     /locus_tag="ON449_002243"
     CDS             2297..3418
                     /locus_tag="ON449_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3934786.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3676..5178
                     /gene="eat(A)"
                     /locus_tag="ON449_002244"
     CDS             3676..5178
                     /gene="eat(A)"
                     /locus_tag="ON449_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3934787.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5206..5772
                     /locus_tag="ON449_002245"
     CDS             5206..5772
                     /locus_tag="ON449_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3934788.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5983..7044
                     /locus_tag="ON449_002246"
     CDS             5983..7044
                     /locus_tag="ON449_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3934789.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7047..7943
                     /gene="murQ"
                     /locus_tag="ON449_002247"
     CDS             7047..7943
                     /gene="murQ"
                     /locus_tag="ON449_002247"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3934790.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7957..9939
                     /locus_tag="ON449_002248"
     CDS             7957..9939
                     /locus_tag="ON449_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3934791.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10004..10450)
                     /gene="nrdI"
                     /locus_tag="ON449_002249"
     CDS             complement(10004..10450)
                     /gene="nrdI"
                     /locus_tag="ON449_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3934792.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3452 a         1862 c         2283 g         2968 t
ORIGIN      
        1 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
       61 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
      121 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
      181 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
      241 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
      301 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
      361 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
      421 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
      481 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
      541 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
      601 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
      661 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
      721 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
      781 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
      841 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
      901 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
      961 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     1021 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     1081 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     1141 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     1201 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     1261 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     1321 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     1381 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     1441 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     1501 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     1561 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     1621 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     1681 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     1741 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     1801 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     1861 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     1921 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     1981 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     2041 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     2101 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     2161 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     2221 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     2281 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     2341 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     2401 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     2461 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     2521 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     2581 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     2641 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     2701 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     2761 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     2821 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     2881 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     2941 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     3001 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     3061 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     3121 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     3181 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     3241 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     3301 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     3361 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     3421 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     3481 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     3541 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     3601 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     3661 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     3721 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     3781 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     3841 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     3901 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     3961 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     4021 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     4081 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     4141 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     4201 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     4261 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     4321 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     4381 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     4441 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     4501 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     4561 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     4621 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     4681 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     4741 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     4801 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     4861 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     4921 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     4981 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     5041 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     5101 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     5161 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     5221 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     5281 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     5341 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     5401 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     5461 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     5521 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     5581 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     5641 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     5701 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     5761 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     5821 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     5881 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     5941 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     6001 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     6061 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     6121 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     6181 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     6241 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     6301 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     6361 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     6421 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     6481 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     6541 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     6601 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     6661 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     6721 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     6781 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     6841 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     6901 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     6961 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     7021 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     7081 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     7141 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     7201 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     7261 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     7321 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     7381 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     7441 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     7501 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     7561 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     7621 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     7681 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     7741 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
     7801 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
     7861 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
     7921 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
     7981 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
     8041 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
     8101 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
     8161 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
     8221 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
     8281 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
     8341 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
     8401 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
     8461 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
     8521 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
     8581 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
     8641 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
     8701 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
     8761 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
     8821 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
     8881 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
     8941 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
     9001 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
     9061 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
     9121 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
     9181 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
     9241 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
     9301 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
     9361 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
     9421 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
     9481 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
     9541 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
     9601 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
     9661 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
     9721 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
     9781 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
     9841 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
     9901 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
     9961 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    10021 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    10081 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    10141 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    10201 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    10261 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    10321 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    10381 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    10441 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    10501 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    10561 tagaa
//
LOCUS       DAJSVP010000089        10465 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSVP010000089 DAJSVP010000000
VERSION     DAJSVP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.093"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            130..2364
                     /gene="pcrA"
                     /locus_tag="ON449_002250"
     CDS             130..2364
                     /gene="pcrA"
                     /locus_tag="ON449_002250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3934793.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2381..4417
                     /gene="ligA"
                     /locus_tag="ON449_002251"
     CDS             2381..4417
                     /gene="ligA"
                     /locus_tag="ON449_002251"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3934794.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4523..4828
                     /gene="gatC"
                     /locus_tag="ON449_002252"
     CDS             4523..4828
                     /gene="gatC"
                     /locus_tag="ON449_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3934795.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4828..6300
                     /gene="gatA"
                     /locus_tag="ON449_002253"
     CDS             4828..6300
                     /gene="gatA"
                     /locus_tag="ON449_002253"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3934796.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6297..7727
                     /gene="gatB"
                     /locus_tag="ON449_002254"
     CDS             6297..7727
                     /gene="gatB"
                     /locus_tag="ON449_002254"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3934797.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7737..8792
                     /locus_tag="ON449_002255"
     CDS             7737..8792
                     /locus_tag="ON449_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3934798.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8903..10276
                     /gene="rlmD"
                     /locus_tag="ON449_002256"
     CDS             8903..10276
                     /gene="rlmD"
                     /locus_tag="ON449_002256"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3934799.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10279..10407
                     /locus_tag="ON449_002257"
     CDS             10279..10407
                     /locus_tag="ON449_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3934800.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         3642 a         1882 c         2322 g         2619 t
ORIGIN      
        1 tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa
       61 ctagctagtt taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg
      121 atgttgttaa tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa
      181 gctgttcttc acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg
      241 agagtgctga cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac
      301 atcctagcga ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg
      361 attctcgcat ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt
      421 attttaagaa gagatgttga ttttatcggg tataaccgga actttacaat catcgattct
      481 tctgaacagc tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa
      541 tatgatcctc gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct
      601 caggattttg caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct
      661 gcttaccaaa aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat
      721 acgattcgat tatttgaaga acatcctgat tcactcacat attatcaaaa caaattccat
      781 tatattcatg tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta
      841 ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc
      901 tggcgcggtg cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca
      961 gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa
     1021 gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga
     1081 aacaagatca cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagat
     1141 cggatgcaag aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac
     1201 cggacgaacg cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat
     1261 aaaatggttg gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat
     1321 ttgaatgtgc tggcaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc
     1381 aaacgaggca tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa
     1441 tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga
     1501 cagcaattag gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg
     1561 acagtgacag aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa
     1621 atccaaaata cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc
     1681 acacaagaat tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa
     1741 gaaaaactaa cggtcttctt aaatgatctg gcacttgttt ccgatattga caatttagaa
     1801 gaagatgctt cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaattccca
     1861 gttgtattcc tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa
     1921 gaaagcgaac tggaagaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa
     1981 gcgttgtatt taacaaatgc tttctccaga acattatatg gacggacgca atataaccga
     2041 ccttctcgtt ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct
     2101 acgcctaaaa aaacacctgt attcgctaaa aaaacagcat atagctataa acagccagag
     2161 acagcggttg ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga
     2221 gataaagtca aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg
     2281 aaggatctag aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc
     2341 tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat
     2401 tgacgaagct gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga
     2461 atactacgta gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga
     2521 attagtagca attgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg
     2581 agtaggcgga aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag
     2641 tttgaacgac gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc
     2701 agtgggacgt gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt
     2761 gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga
     2821 aaacatcact gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat
     2881 gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca
     2941 agacagagaa gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc
     3001 tttgcgtcag ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac
     3061 agtagctgat ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga
     3121 aaaaattgga ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg
     3181 gtcatacatt gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat
     3241 cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt ggttttactg tcaaagcacc
     3301 gagatgggca gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat
     3361 cgaatggaca gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg
     3421 agtagcagga acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa
     3481 ggatatccgc ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt
     3541 ttcgcaagtc gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca
     3601 ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat
     3661 caatcctaaa tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc
     3721 gatgaatatc gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt
     3781 agcagatgtt gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat
     3841 aaaagaaaaa tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt
     3901 cgagcggttg attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt
     3961 agcagaacat ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc
     4021 gcttgatacg atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga
     4081 agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct
     4141 tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac
     4201 tggtaaacta acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg
     4261 gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg
     4321 cagcaagctg accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc
     4381 agatgcattg gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat
     4441 ttcaataaac aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac
     4501 tcgtttagaa agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa
     4561 attagcaaaa cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa
     4621 aatcatcgat atggttgaaa tgctggaaga agtagataca gaaggtgttc catttacgtc
     4681 gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg
     4741 cgatgaatta ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat
     4801 catcgataat ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa
     4861 ctgcatcata tgcttgtatc gaaagaaatc actgtacaag atttgacaaa agcaacattt
     4921 gaacgaatca aagaaacaga gcctgaggtc gaagcattca ttacattgaa cgaagaaaaa
     4981 gcattggaac aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct
     5041 ggtattccaa ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca
     5101 tctaagatcc tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat
     5161 caagcagata tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt
     5221 acagaaactt cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt
     5281 ggctcttctg gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt
     5341 agtgatactg ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa
     5401 cctacttatg gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa
     5461 atcggaccaa tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt
     5521 tatgacgaaa aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg
     5581 acaggcgaca tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg
     5641 gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct
     5701 gttgtggaag aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt
     5761 gcctcatcag aagcaagttc caacttgcaa cgattcgatg gtatccgcta cggttatcgc
     5821 tcagaaaatg tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt
     5881 gaagaagtaa aacgccgtat catgttaggt acattctcct taagtgctgg ttattatgac
     5941 gcttacttca aaaaagcggg acaagtccgt acattgatca aacgagattt tgaaaaagtc
     6001 tttgcagatt atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt
     6061 gagaacatta acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac
     6121 ttggctggac ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga
     6181 ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt
     6241 gaacaagcaa cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga
     6301 attttgaaac agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt
     6361 tctctccagc gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact
     6421 ggggttatcc aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag
     6481 cagctttggc attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact
     6541 tttacccaga taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg
     6601 acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc
     6661 atttagaaga agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt
     6721 tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag
     6781 aagaagcata cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg
     6841 atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg
     6901 gacaagaaga gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga
     6961 aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa
     7021 tccaacaaga aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca
     7081 aagaagggtc tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg
     7141 atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg
     7201 aacgctatat ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa
     7261 aagaaatgtc tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat
     7321 ctaactggtt gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg
     7381 agacaaaatt gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga
     7441 tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc
     7501 aagaagtagt tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa
     7561 tcattaatga aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag
     7621 atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc
     7681 ctggtgtcgt gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga
     7741 aaaaagcacg cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg
     7801 cggatatcct acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac
     7861 cagaaccaga ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag
     7921 tagtggcagc tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg
     7981 aacaccgtcc taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat
     8041 taaaaattcc tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg
     8101 taaaaatgga catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct
     8161 atttgacaga attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg
     8221 cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga
     8281 agtatgatga aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact
     8341 ctgttggagg attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt
     8401 taatcattgt gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga
     8461 atggcggtcg acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg
     8521 ttgaaacaga agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg
     8581 gggaagcacc gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg
     8641 atcagattcc tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca
     8701 aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa
     8761 tagcagacga aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg
     8821 tcagtgccgg ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa
     8881 aataggctta aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata
     8941 tacagtcgaa atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta
     9001 tccattattt atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc
     9061 aggcaataag tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca
     9121 gcctgtagaa aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta
     9181 tgaacaacag cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa
     9241 gatgccagaa gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa
     9301 taaagcacag atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa
     9361 gaatagccat gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca
     9421 agcaatcaaa gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga
     9481 aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat
     9541 gatggtcgtt ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc
     9601 tattcatgaa gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac
     9661 aaatgtgatt ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca
     9721 gctattagga aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca
     9781 agcagagtat ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt
     9841 gatcgacgct tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa
     9901 ggtgtatggg atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa
     9961 taataaaatc gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg
    10021 gcaagaaaaa gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga
    10081 tcatcatttt attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg
    10141 taatccggca acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa
    10201 gaaagttcag cctgtagatc ttttcccgca aacccatcat atcgaattag tcgcttcatt
    10261 cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa
    10321 caaggtggag gccgatccaa acaaatggaa acacctaaga atgatcgtaa aaacatgaga
    10381 aaaggcccta aaatctttaa taaatagacg tgctaaagaa agactaagaa gtgttaactt
    10441 ttgttattgt ttcttagtct tttta
//
LOCUS       DAJSVP010000090        10404 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSVP010000090 DAJSVP010000000
VERSION     DAJSVP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.094"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            169..318
                     /locus_tag="ON449_002258"
     CDS             169..318
                     /locus_tag="ON449_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3934801.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="ON449_002259"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="ON449_002259"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3934802.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="ON449_002260"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="ON449_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3934803.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="ON449_002261"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="ON449_002261"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3934804.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="ON449_002262"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="ON449_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3934805.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="ON449_002263"
     CDS             4735..5817
                     /locus_tag="ON449_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3934806.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="ON449_002264"
     CDS             5817..6620
                     /locus_tag="ON449_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3934807.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="ON449_002265"
     CDS             6690..7430
                     /locus_tag="ON449_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3934808.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="ON449_002266"
     CDS             7441..8445
                     /locus_tag="ON449_002266"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3934809.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8490..10334)
                     /locus_tag="ON449_002267"
     CDS             complement(8490..10334)
                     /locus_tag="ON449_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934810.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3355 a         1712 c         2097 g         3240 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8521 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8581 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8641 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8701 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8761 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8821 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8881 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     8941 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9001 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9061 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9121 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9181 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9241 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9301 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9361 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9421 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9481 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9541 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9601 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9661 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9721 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9781 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9841 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9901 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
     9961 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10021 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10081 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10141 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10201 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat
    10261 ataatgatta tgctccactt tatacgtaaa taaaaagatt cttttttatt atatatgtta
    10321 ttaaaaaaag acataaaggt attcctttct aaaacttgta atgttagggt aaacgttttt
    10381 taaactaacg tcaattattt ataa
//
LOCUS       DAJSVP010000091        10291 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSVP010000091 DAJSVP010000000
VERSION     DAJSVP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.095"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..769)
                     /locus_tag="ON449_002268"
     CDS             complement(<1..769)
                     /locus_tag="ON449_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR3934811.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="ON449_002269"
     CDS             complement(974..1324)
                     /locus_tag="ON449_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934812.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="ON449_002270"
     CDS             1919..2365
                     /locus_tag="ON449_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3934813.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="ON449_002271"
     CDS             2989..3981
                     /locus_tag="ON449_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3934814.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="ON449_002272"
     CDS             3997..4881
                     /locus_tag="ON449_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3934815.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="ON449_002273"
     CDS             4930..6537
                     /locus_tag="ON449_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3934816.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="ON449_002274"
     CDS             6538..8022
                     /locus_tag="ON449_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3934817.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8199..9191
                     /locus_tag="ON449_002275"
     CDS             8199..9191
                     /locus_tag="ON449_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3934818.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9243..10112
                     /locus_tag="ON449_002276"
     CDS             9243..10112
                     /locus_tag="ON449_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3934819.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3253 a         1632 c         2200 g         3206 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     8101 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     8161 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     8221 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     8281 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     8341 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     8401 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     8461 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     8521 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     8581 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     8641 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     8701 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     8761 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     8821 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     8881 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     8941 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     9001 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     9061 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca atagaaaaac
     9121 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     9181 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     9241 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     9301 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     9361 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     9421 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     9481 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     9541 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     9601 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     9661 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     9721 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     9781 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     9841 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     9901 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     9961 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
    10021 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
    10081 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
    10141 ataccggaag tttaaaaggg tattctgaaa cttaaatagt ctcaattcat tcttctttct
    10201 tgagaatctg agtagagact tttattctgt ttcaaattaa tataattacg aaatgatatt
    10261 gagtacaaga aagttctttt taccagatct t
//
LOCUS       DAJSVP010000092        10133 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSVP010000092 DAJSVP010000000
VERSION     DAJSVP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.096"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            122..628
                     /locus_tag="ON449_002277"
     CDS             122..628
                     /locus_tag="ON449_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3934820.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            645..1544
                     /locus_tag="ON449_002278"
     CDS             645..1544
                     /locus_tag="ON449_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3934821.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1631..2515
                     /locus_tag="ON449_002279"
     CDS             1631..2515
                     /locus_tag="ON449_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3934822.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2707..2778)
                     /locus_tag="ON449_002280"
     tRNA            complement(2707..2778)
                     /locus_tag="ON449_002280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2743..2745),aa:Arg,seq:ccg)
     gene            2838..4172
                     /gene="rpoN"
                     /locus_tag="ON449_002281"
     CDS             2838..4172
                     /gene="rpoN"
                     /locus_tag="ON449_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3934823.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4321..5412
                     /locus_tag="ON449_002282"
     CDS             4321..5412
                     /locus_tag="ON449_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3934824.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5453..6454
                     /gene="gap"
                     /locus_tag="ON449_002283"
     CDS             5453..6454
                     /gene="gap"
                     /locus_tag="ON449_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3934825.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6564..7754
                     /locus_tag="ON449_002284"
     CDS             6564..7754
                     /locus_tag="ON449_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3934826.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7858..8613
                     /locus_tag="ON449_002285"
     CDS             7858..8613
                     /locus_tag="ON449_002285"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3934827.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8729..10027
                     /gene="eno"
                     /locus_tag="ON449_002286"
     CDS             8729..10027
                     /gene="eno"
                     /locus_tag="ON449_002286"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3934828.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3549 a         1869 c         2099 g         2616 t
ORIGIN      
        1 atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa
       61 aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag
      121 aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa
      181 tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga
      241 taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga
      301 aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc
      361 attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat
      421 cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat
      481 agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga
      541 aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt
      601 gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag
      661 aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag
      721 aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa
      781 agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc
      841 ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca
      901 ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac
      961 cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat
     1021 actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa
     1081 cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg
     1141 tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca
     1201 caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct
     1261 atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc
     1321 catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac
     1381 ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt
     1441 ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg
     1501 tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg
     1561 actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag
     1621 aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt
     1681 gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc
     1741 ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc
     1801 aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca
     1861 gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt
     1921 cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt
     1981 aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc
     2041 tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca
     2101 tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac
     2161 tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt
     2221 cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga
     2281 agattggaag acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga
     2341 tggggcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt
     2401 gatcgattat ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca
     2461 tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag
     2521 acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact
     2581 tgcaaaggct taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg
     2641 gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca
     2701 gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat
     2761 ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa
     2821 ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac
     2881 agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg
     2941 aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg
     3001 agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga
     3061 caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac
     3121 aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat
     3181 acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag
     3241 ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag
     3301 taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc
     3361 cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga
     3421 aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt
     3481 tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta
     3541 ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa
     3601 aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag
     3661 ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa
     3721 cgatcctaca acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa
     3781 aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa
     3841 ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa
     3901 cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta
     3961 cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg
     4021 atcaagaaga caaaaacaag ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag
     4081 atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga
     4141 gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc
     4201 actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga
     4261 ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat
     4321 atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg
     4381 gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc
     4441 tataaaattt tacgaaatat ctattggatg cagccgattg gacgtcgaag tttatcggaa
     4501 agtatgggga tcacagaacg agtattgcga acagaaacag atcttttgaa aaatctgaat
     4561 ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa
     4621 agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca
     4681 gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa
     4741 gtggctgatg cattcggcga agcgctgagc gaagccctag accgactact tccagaaggc
     4801 gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct
     4861 aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa
     4921 gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac
     4981 caccgtgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat
     5041 gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt
     5101 attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg
     5161 ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa
     5221 gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc
     5281 atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat
     5341 gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg
     5401 gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt
     5461 taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga
     5521 tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca
     5581 cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg
     5641 ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt
     5701 accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa
     5761 atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg
     5821 cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga
     5881 aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt
     5941 gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga
     6001 ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc
     6061 tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag taattcctga
     6121 attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac
     6181 agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa
     6241 agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt
     6301 tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga
     6361 caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt
     6421 agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt
     6481 atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat
     6541 aagtttattc aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag
     6601 acaaaaaagt tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg
     6661 acgacactcg tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa
     6721 aagcaatcct tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat
     6781 ctttagctcc agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac
     6841 ctgaaactcg cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg
     6901 tcgtattcga aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata
     6961 cagaactagg taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta
     7021 cagctcaccg tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg
     7081 ctggattctt gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac
     7141 gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa
     7201 acttgattcc taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg
     7261 aagcaaaagg aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa
     7321 aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac
     7381 cagaattttc aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga
     7441 tggctttaga catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa
     7501 aaacagttgt ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta
     7561 caatcggtgt ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg
     7621 gagactcagc tgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa
     7681 caggtggtgg cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa
     7741 tcaacgataa ataaacgacc aagtttgctc aattctgacg agtaatcgat caaaaggtga
     7801 agggagtttt catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg
     7861 cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa
     7921 tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga
     7981 tctcctgcat tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc
     8041 tcagcacaaa actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca
     8101 gctcttgctg atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat
     8161 ttccatgaaa cagacgaaga catcaataaa aaagcaaaag ctatctttgc aaatggtatg
     8221 acaccaatcc tatgctgcgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa
     8281 tggatcgaag gacaaattac agctggttta gctgatcttt cagctgaaca agtgtcaaac
     8341 atggttattg cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa
     8401 atcgctgacg aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa
     8461 gtatcagaag cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa
     8521 tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat
     8581 tcatttttgt cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag
     8641 aaatgtttgc actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga
     8701 aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga
     8761 gatcttagac tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc
     8821 ttttggccgc ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt
     8881 acgtgacgga gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt
     8941 aaacaacatc attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga
     9001 caaagctatg atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat
     9061 tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata
     9121 ccactaccta ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa
     9181 cggcggatca catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg
     9241 cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc
     9301 aatcttgaaa gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa
     9361 ccttggttca aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta
     9421 tgtacctggt aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa
     9481 agaaaaaggt gtttacgtac tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat
     9541 gatcaaattc tacgaagaat tagtttctaa atatccaatc atctcaatcg aagatggatt
     9601 agacgaaaac gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca
     9661 attagttggt gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa
     9721 aggaattgct aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt
     9781 cgaagctatc gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg
     9841 tgaaacagaa gattcaacaa tctctgatat cgctgttgca acaaatgctg gccaaatcaa
     9901 aactggttct ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga
     9961 agaccaactt ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa
    10021 caaataattc ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat
    10081 cacttagtta agtaatcgaa ctctctactt gctgaaatat cagcaggtag aga
//
LOCUS       DAJSVP010000093        10096 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSVP010000093 DAJSVP010000000
VERSION     DAJSVP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.097"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..223)
                     /locus_tag="ON449_002287"
     CDS             complement(26..223)
                     /locus_tag="ON449_002287"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3934829.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(369..1247)
                     /locus_tag="ON449_002288"
     CDS             complement(369..1247)
                     /locus_tag="ON449_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3934830.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="ON449_002289"
     CDS             complement(1286..2404)
                     /locus_tag="ON449_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3934831.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="ON449_002290"
     CDS             complement(2401..4500)
                     /locus_tag="ON449_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3934832.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="ON449_002291"
     CDS             complement(4551..5372)
                     /locus_tag="ON449_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3934833.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="ON449_002292"
     CDS             complement(5369..6178)
                     /locus_tag="ON449_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934834.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="ON449_002293"
     CDS             complement(6199..6693)
                     /locus_tag="ON449_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934835.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="ON449_002294"
     CDS             complement(6705..7193)
                     /locus_tag="ON449_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3934836.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="ON449_002295"
     CDS             complement(7270..9966)
                     /locus_tag="ON449_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3934837.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3068 a         2270 c         1661 g         3097 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattc
//
LOCUS       DAJSVP010000094         9976 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSVP010000094 DAJSVP010000000
VERSION     DAJSVP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.098"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..2128
                     /gene="acm"
                     /locus_tag="ON449_002296"
     CDS             <1..2128
                     /gene="acm"
                     /locus_tag="ON449_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR3934838.1"
                     /translation="IITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVK
                     FSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVF
                     NENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSV
                     FYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLG
                     EQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPE
                     QINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIG
                     IPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPL
                     SSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNW
                     EPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENG
                     LVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYR
                     FTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNF
                     PEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKK
                     SSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2400..2885)
                     /locus_tag="ON449_002297"
     CDS             complement(2400..2885)
                     /locus_tag="ON449_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3934839.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2975..3320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3365..4006
                     /locus_tag="ON449_002298"
     CDS             3365..4006
                     /locus_tag="ON449_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3934840.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4025..4660
                     /locus_tag="ON449_002299"
     CDS             4025..4660
                     /locus_tag="ON449_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3934841.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4678..5493
                     /locus_tag="ON449_002300"
     CDS             4678..5493
                     /locus_tag="ON449_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3934842.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5812..7248
                     /locus_tag="ON449_002301"
     CDS             5812..7248
                     /locus_tag="ON449_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3934843.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7437..9884
                     /locus_tag="ON449_002302"
     CDS             7437..9884
                     /locus_tag="ON449_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3934844.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3466 a         1663 c         2156 g         2691 t
ORIGIN      
        1 tattattact aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga
       61 tatcagcagt aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa
      121 cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct
      181 ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc
      241 attaatgatt gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt
      301 tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat
      361 acaggccaga aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag
      421 cggtgataaa acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt
      481 tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt
      541 gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca
      601 aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca
      661 gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc ccggcgctac
      721 atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa
      781 tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt
      841 tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag
      901 tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg
      961 cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt
     1021 cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc
     1081 gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga
     1141 aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc
     1201 cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac
     1261 gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt
     1321 taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg
     1381 attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg
     1441 aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc
     1501 aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc
     1561 agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac
     1621 gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa
     1681 agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat
     1741 ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat
     1801 caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc
     1861 gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa
     1921 attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa
     1981 gagagctgta cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag
     2041 taaaaaatct agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat
     2101 attcttacaa aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact
     2161 gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct
     2221 tcttttttcg tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta
     2281 gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata
     2341 ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat
     2401 tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa
     2461 aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga
     2521 tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat
     2581 ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa
     2641 gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca
     2701 ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag attttttact
     2761 tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact
     2821 ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga
     2881 ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga
     2941 aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc
     3001 cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac
     3061 acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc
     3121 tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa
     3181 ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc
     3241 tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc
     3301 acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa
     3361 gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt
     3421 gaaattattt ttatttacct tgattggctc attaccatta gggattgttt tagcattagg
     3481 attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg
     3541 gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat
     3601 cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt
     3661 tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca
     3721 agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa
     3781 gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat
     3841 ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc
     3901 gactttactg cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac
     3961 aatcgtaatg aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag
     4021 aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga
     4081 tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg
     4141 taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta
     4201 tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt
     4261 cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc
     4321 accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc
     4381 ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca
     4441 aaaacagcgg gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga
     4501 tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc
     4561 attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa
     4621 tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg
     4681 aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg
     4741 aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata
     4801 ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga
     4861 tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag
     4921 cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg
     4981 aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg
     5041 gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg
     5101 aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa
     5161 ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat
     5221 gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac
     5281 gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct
     5341 tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa
     5401 atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa
     5461 tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt
     5521 gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc
     5581 tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta
     5641 agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa
     5701 actaagcgac tttttcacaa cctcctttac ataattttat tttgttgtgg aaggggtttc
     5761 gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac
     5821 aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa
     5881 gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg
     5941 atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt
     6001 tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta
     6061 gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt
     6121 tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat
     6181 cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc
     6241 attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat
     6301 tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca
     6361 catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta
     6421 gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca
     6481 agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct
     6541 gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat
     6601 ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc
     6661 gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg
     6721 gcgacgaatg cattcaatcc attaaacgga gttggtgcag gaaaaatgaa tacgacagga
     6781 aaaaaaggtt catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca
     6841 tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg
     6901 cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag
     6961 tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa
     7021 agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc
     7081 acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt
     7141 gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt
     7201 tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta
     7261 aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt
     7321 aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac
     7381 tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg
     7441 cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac
     7501 agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct
     7561 tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg
     7621 caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca
     7681 ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt
     7741 atttcggtat cttattattg ttacatgccc atttatttgg caaactccat actggggaac
     7801 caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag
     7861 acaataacgt tggtggtggg atgattggag caatccttta tcattttact tacttcttga
     7921 ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt
     7981 tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac
     8041 aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga
     8101 gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag
     8161 aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg
     8221 ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg
     8281 acagttttca agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa
     8341 aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat
     8401 tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt
     8461 tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata
     8521 tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca
     8581 gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta
     8641 aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg
     8701 aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta
     8761 tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag
     8821 tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc
     8881 acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca
     8941 caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa
     9001 tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc
     9061 ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa
     9121 aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa
     9181 acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat
     9241 tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc
     9301 aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg
     9361 ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg
     9421 gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag
     9481 atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt
     9541 ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg
     9601 aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag
     9661 atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc
     9721 ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg
     9781 aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc
     9841 aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag
     9901 ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg
     9961 tttttttcca tctttt
//
LOCUS       DAJSVP010000095         9790 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSVP010000095 DAJSVP010000000
VERSION     DAJSVP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.099"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            243..437
                     /locus_tag="ON449_002303"
     CDS             243..437
                     /locus_tag="ON449_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3934845.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            440..1642
                     /locus_tag="ON449_002304"
     CDS             440..1642
                     /locus_tag="ON449_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3934846.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2225..3856)
                     /locus_tag="ON449_002305"
     CDS             complement(2225..3856)
                     /locus_tag="ON449_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3934847.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3870..5543)
                     /locus_tag="ON449_002306"
     CDS             complement(3870..5543)
                     /locus_tag="ON449_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3934848.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5556..5873)
                     /locus_tag="ON449_002307"
     CDS             complement(5556..5873)
                     /locus_tag="ON449_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3934849.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5898..7298)
                     /gene="lacG"
                     /locus_tag="ON449_002308"
     CDS             complement(5898..7298)
                     /gene="lacG"
                     /locus_tag="ON449_002308"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3934850.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7535..8320
                     /locus_tag="ON449_002309"
     CDS             7535..8320
                     /locus_tag="ON449_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3934851.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8323..9015
                     /locus_tag="ON449_002310"
     CDS             8323..9015
                     /locus_tag="ON449_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3934852.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9289..9660)
                     /locus_tag="ON449_002311"
     CDS             complement(9289..9660)
                     /locus_tag="ON449_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3934853.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3093 a         2086 c         1721 g         2890 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatgagttg ggaagtcctg aacaagtgat ggattttcaa gaaagtaccg ttcattatac
      181 aaaaataaag aaataacact attacccaat gatagtgttt tctttgaaag tgagtttata
      241 caatgaaaaa tacaatttgt cgaaaagatt tagaggctct aggatttaaa acacatcaag
      301 ctaccacaat aatccgacaa gcaaaaacaa gaatggtaaa tcagggatat ccatattata
      361 ataataaaag agttggggta gtaccccgac aagtaattga agaaattata ggtgttcctc
      421 tttcacagga ggaataacta tgaaaacaaa atacccttgt gtgtatcaag ataaaaatgg
      481 tcgcttttct tatctagttg agttaggaac agatcaagtg actggaaaga ggatccagaa
      541 gaaaggaaca aaagattctc taggaaaacg attctcatca gctcgtgaag cacacaaaga
      601 agtcatgcgt ataaaaaatg aatacatggt tgtaaatgga tttgcgaatt atgatttaac
      661 ttacaaagaa tttatggagc agtcttacgt tccacactac aaagctagcg tacaaaaaag
      721 tacttggagt tctcgtgagt gtggattgat tcagattact gaatttttcg gtgataagaa
      781 gcttcgtgaa atcaatgtgc gagattgtga aaattatcga atttggttat tgaacgatag
      841 cggatactca caagcataca gttcacttat gtacggtatg tttcgtaaat cattagacta
      901 tgcggtcacc ctgcaatttc tgaatgagaa tatttccaaa agaacaaaag caattccaaa
      961 aggaaaatca atcgttcctt actggacaaa agaagaattt gaagaagttt tggcaaaaat
     1021 ttataaagat gatttctacg aacatatgtg ttttttaatg atctggctat attacatgac
     1081 tggtgttcgt gtaagcgaag gattagcact taattggaat gacgtggact tcaaacgtaa
     1141 aaagttgagg gttcatcaca ccttggaaat gacaaatcaa aaagatttta ttcggaaacc
     1201 ttacacaaaa actgaaagcg gcttgcggat catttctctg gacgatgata caattgaagt
     1261 gctaaaaaag tggaaagtta ttcaaaaaaa tcatggtgta gaacaattta ttcttagcta
     1321 cactgacctc ccactctatc gttcaactgt tcaacgaatt attcagcgtt atgccaaact
     1381 agctaaggta cccctaatac aaggaaaagg attacgtcat tctcatgtta gctacctaat
     1441 caatgagttt aacgcagaca ttctggtcgt gtcgcaacgg ttaggccatt ctagccctga
     1501 aatcacttta aagcactacg ctcatctatg gagccgtaat gatgaaggaa ttgcagaaca
     1561 aatgacagga aacattaaat ttacctgtgc aacaaaatct aaagttgcaa actttaatgg
     1621 aaatcaagca gtaaaaatct aaccctacca tttccctacc aaaatgagga ataggctgaa
     1681 aaagcactga tataatagct tctttacgag gctactcata ttgagtggga ataaagagcc
     1741 aacataatat ggagggggaa tccctataaa tcaacattct tcaatagcta aaatgcttga
     1801 ttttcattaa aaataagatg gttccctacc aaaagagact ataatataag ttaatttgtt
     1861 tcattcttat cataaaaaga agttctcgaa aaacgaactg ttttccgaga acttttttct
     1921 atccatctaa gtgctcaaat catattcacg acggaattct aaggttaccc tttcttaaac
     1981 aaactttatg ttatctgcac aactttttgc aattttttca ttgcttctta cgaaatcgtt
     2041 gaactctctt tagcataata caaagtgtag atgaagtctg gtgctttact cgttttggta
     2101 aaaatgactg gaaatcgttt ctggttattt ttggcaaagg cgaggtgcag gttcgattcc
     2161 tgccttctac gctaacgatg tggctgaagg gaacctccga tcttgaatcg aaacgatccg
     2221 cagtctattg tttcaccaat gaaacaaaat caacgttcgt ccaagcattt cctgaagcct
     2281 tgctataaac cccaatttca caaacacctg ttgtaattgg aatgtctttg attgtaattt
     2341 ttgtccattc acttgtttta acaaccgcag tatttagttc cgctccacca taatttttga
     2401 tatataactg attagtatca aactctcccc cagacattac ataggcactc aatgtgtaag
     2461 ttcctttgct tatgccacta acattttgat aagctgatgc ctcgtatgct tgatcctgcc
     2521 aaaaagttag tttgtagtcc ccaaaaaaag tgcccgcttc cgttttgatc gtacccttag
     2581 cggttccttg ttgaaaccag gtttgccaat tggaaggact ggtcgttggt ccatcgcttt
     2641 cgaaacttgc atttttaact agatttattt gcgcccctgc ttgtttaaac gcacgcaagc
     2701 ttggcaaggc gtttccagca aaatcaaaca tcgcttggtt gtcccattcg tttcccatag
     2761 tggattgaac actgagataa ttcatacctg cttgtgttgc ccaactcaca tttccgttgt
     2821 accaaagagg ttcccaatag aagaagcccg ccccgcgatt attcggtact gcctgaatcc
     2881 gatcggcaag atccagcaaa tactctgctt gtcctgtcgg tgttggttca tatccccccg
     2941 cagctgcttc ggcttcacca aaagcatttg gcatagtatc ggcattcgct gttgtatgcc
     3001 cataagctgt ttcaactacg atcacttctt taccataccg ttggctgatc gcattcatat
     3061 tggtttgaag ttcattcatt gaaccgtgcc aatacggata atacgacact ccaattaaat
     3121 caaatgacac acctcgactc gtgatttcat caaaccacca agtaaatgct cctgtatcgc
     3181 caccatcagc taagtggagc attactggag ttgttttagg ttgagtgtct cgaacagctt
     3241 ggataccaga cttcaaaaaa gcagccaatc gatcaaattc tcccccgcct tctccccaag
     3301 acttgccata aggccagagc attccgctat tcagttcatt gccgatctga accatatcag
     3361 gataaacgtc cagctgcttc atttccgaca gaacgttctt tgtatagtcg tacaatgccg
     3421 tattcatttc ttcaaaggtc agaccttgcc atgctttagg aagattctgt ttcccaggat
     3481 caacccaaaa atcactgtag tgaaaatcca gcagcacctt aagtcccaga ttttttgctc
     3541 gttttgcaag gtcaatcgtc gtttccagat catttgtccc tgctccataa ctttctccat
     3601 tactattata aggatcctgc cacaaacgca gtcgaacata gttgacacca ttatttttta
     3661 aaatctctaa ggcatctttt gaaatatcgt cttcataata ttttgcacca gatttttcca
     3721 tatctgacag gatcgaaata tctgctccgc gaataaattc gctggcagat gcttcaacag
     3781 tttttgaaac agcccccaaa gcccctaaca cagctgcaaa taaaacagct ttacgcaacc
     3841 aaattcccat tttcattatg gctcctcctt tattgaaatt ggttcagaat aaactgaaca
     3901 gctttttcag gatctctcgt taatccgata tattctgctc cttttgtcgc aaccagcttg
     3961 attcctaagc gatcggtgtc ttctttgata tcatcatagt aagaattgac ttgcggtgct
     4021 aagaccacca tatcaaaatc ctgcagcatt tcgtaatgag aaccataagc acccgccatt
     4081 gcactgatcg gtgctttcga ttcagatgca ccttttttta aagcattagc taacatcgca
     4141 cttgtccctg cgccagcaca aagtaccaac acattgattt ctttatttgc atcgctgata
     4201 acttgactcg ctgccacagg tacttcctca cgtacatcgg cagaagcacc ttcctcggtt
     4261 gcgatcacct ctgctttttg tgcttctttc tctactaaga cagcatcgta tgctttgcaa
     4321 aatggtagat agatcaggaa gtcaacgacg attagaaccg ctgctagaac aaaagctaag
     4381 atacccatgc ccgttcctag aaccaaaccg atcggtgctg gagttgccca aggcaaaacg
     4441 tacataaagc tgttcatgcc caatacatcg acaaaaaact tgaacaggca aacgtttaca
     4501 acaggtgtta acaaaaatgg aataaaaaaa taagggttca aaatcatggg tgccgcaaat
     4561 agcaatggtt cgttgacagc gaaacttact ggaataaagg aagctttccc aactgccttc
     4621 aactgcttag acttagcaaa gagtaggaag ataaaaggaa caaccaatgt tgccccagtt
     4681 cctcccatag ttcctacaaa attccctaaa cccaccgtca gtactttaga agcttgttca
     4741 ccggaattga acaattgcag attggtttcc acattggcat agataattgc cgcaattgcc
     4801 ggctccacga tcgacggtcc atgaacaccg acaaaccaaa acagcgccat cgctccccag
     4861 ataatagcga ttcctaagta cccgtcagct gctgtaaata gcggctgcag caccgtaatg
     4921 attgcttctg caaatgagat ttggaagatt tgtcgaatgc ctaaatcaat taaaacggcc
     4981 gcaaatactg aaaaagagaa aggaaaaata tctcggaagg tttgcgaaat cgttccagga
     5041 acttctttag gcattttaat ggtaatatct ttcagcacac aaaacttata taaattaact
     5101 gttaaaaatg cagcgacaaa ggaagccagt agtccctttg tccccatata ttccgttgaa
     5161 aatccacctt caatcggaga gacgcttaac aacaggaaac ttaccacaga ggccagcatc
     5221 acagaggtgg tgttgattac tttgtttttc ggtaagcgac gattcatcga atcagccaag
     5281 cagcgagcag tggttgctga aacgagtaac cctacaacac ccatcgaata attatagact
     5341 ttccatagcc atgtggagac agattcaggc cattggtaac caaaaatctc aggaaccgca
     5401 gcaatcaaaa tgaaaatgct tgaaaataaa atagctggca tcgctgttaa aaatccgtca
     5461 cgaacggctc ctaaataact attacgtgag attttttcaa aaaatggttt ccctttttcg
     5521 atttgtttaa tgattccatc catagcgttc ttctcctatc cttgattctg tctgtacagt
     5581 acaataaagt cttgaatcag atctctcagc aataaagtgg tcattaaatg atcctgccca
     5641 tggataaaaa tgaatcccat ctccatctct ttcccagatg cttcttccgc taaaattttt
     5701 gtttgcgaat tatgtgcaag ggttaaattt tcgtcggctt ctttcaacgc cgaatcaaca
     5761 tgttcaaagt ttccttgtct tacttctttc aataatttca ataatgtact gcgagcgtcg
     5821 cctgaatatg caacgatctc aaaaccaagc atttgtaatt cctctttatt catcttttct
     5881 tcctccatta tcacttatta aataatcgtt ttggtttcac tgaccaactt gtaccagtaa
     5941 gctgattctt ttgggtaacg cttctgggtt tcaaaatcaa cataaaacaa gccatacctt
     6001 ttgttatacc catttgtcca tgagaaaaga tccataagcg accacaaaaa ataccccttc
     6061 acatttaccc cggctgttat cgccttgctt aaggattcca aatagactct caaatagtcg
     6121 attctcggct gatccataat aatgccatct tcaaactgat ctttataccc catcccgttc
     6181 tcagtgatat agattttgtt gtaatgtggg taatcgcttt taattcgcag caacaagtca
     6241 tatagacctt ctggataaat cagccagtcc caatcagttc gaggaattcc ttctttataa
     6301 atacgttctc cgattccctt cactttataa acagaggtcc ctttttcacc cgtaccgtta
     6361 tgatggattg cattttctcc atcgtaagct ttgacaaaat ggcattggta gtgattgatc
     6421 cctaagtagt cattacgcgt ggatgctttt ttcaattcaa cgaaatcttc ttcaggaaaa
     6481 tgatagctgg cctgatttgc ttcacatatc tcatcaagag cagtcaaggt ttcagttgaa
     6541 taatagccta aataggtagc atctaataag aaacggatcg acaatgcatc gtctaaaaag
     6601 gcagcatgct tatcttccgg cgcgtctgtt gccgcatatt tcgtctctaa tgagtgaact
     6661 acaccaattt cacccggaag ttcgttttct ttgaaatagt tcactacacg ggcatgagca
     6721 accatcatat tatgaagaca ggcgacgatc tttgtaaaat catatttgat tcctggtggg
     6781 aaaacaccta acaaatattg attggttgca actggataga tttcattaaa ggtactccac
     6841 accttgactt ctttgaattc atgaaaacaa aaaatggcat aggaaacaaa tgcttctatt
     6901 gtttctcgat tcaaaaaatc tccatggtca aacaaacgtt taggggtatc aaaatgatgc
     6961 aacgtcacaa acggcgttac gtttcttttc ttacattctt cgaacacacg atgataaaaa
     7021 gcgattcctt ctggatttgg tttcccagct ccatcaggaa aaatgcggct ccaagcaatg
     7081 gataatctta gaccatttac accaaaacgt tcacacaatt caatgtcctt tgcatattga
     7141 tgataaaaat cacttgccgg atcgggactg aatcgccctt gttcctctaa aaagtcatcc
     7201 caagcaacag cgcctttgcc accttcctta gtggctcctt caacctgata ggctgctgtg
     7261 gctccgccaa aaataaaatc ttctggtaat ttaagcatgt acatctctcc tcaaatctat
     7321 aatcagtttt cgctctttct aactgattgc attgtaaacg catccaaaac atttgtcaac
     7381 aaaaacaaac acaaataatc ataaaaacca gaaaacagca ataaaatcta ttgtaacaaa
     7441 cataaaaacc accctaaatg tttacttttg ttcgaattag attgactgtt gtccgtttat
     7501 atgatagatt atccttaaat tagaggtgaa gacaatgttg aaaaaagaaa gattgcttac
     7561 tatcgtagaa atggtcaata aaaaaggaat ccttaccgtt aatgaaatca tcaatcaatt
     7621 agatgtttct gatatgaccg ttcgcagaga tttagatgaa ttagaaaaat cgggcaagct
     7681 gctgcgtgtc catggagggg ctcaaagtct ttcctacact ttagatcaag aactttctca
     7741 cactgaaaaa gccactctgc agattcaaga aaaaaaagaa attgctgcca cagcagcctc
     7801 cttgattcac gagggagaaa cgattttctt aggaccaggt acaacaatcg aactattggc
     7861 gacgcattta ctgaacaaga aaattcggat cattacaaat aattatccag tttttgatat
     7921 tcttgctcaa gcagatggac cagaaattct actgattggc ggtgatttcc ggaaaaatac
     7981 cggcgcattt gtcggaccga tcaccaacga caatttaaga agactaaagt ttacaaaagc
     8041 atttatcagt tcaaatggcg ttcacaacga agcgatctct acttacagta ccgaagaagg
     8101 agaagcacag cagatcgctc taaataattc ccgtaagaaa tatctactcg ttgataataa
     8161 aaaattcaat cgagatgatt tttatgtgtt ttataatttg catgatttcg atctgctcat
     8221 tacggatcgc cagatctcgc gggaggttca agagcactat gcacaatatg tcgatctgca
     8281 aattgctgat tcaaccgaaa ataccactat aggagcgtaa ctatgaaagg gattatattt
     8341 gatttcaacg gaacaatgtt tttggactct catctgcatg aagctgcttg gctgcatatg
     8401 atacatgatc atacaaaaga tggtttatca gacgaggata ttttaaaaaa tattcatggt
     8461 cggacgaata cggagatttt gaaacatttc atttcagagc atttgaccgc ctctgagatc
     8521 gccgtattgt cagaagaaaa agaaagctat tatcgtatgc tttgcttgca aaatgaaaac
     8581 gagcttcagt tgacaaaagg gcttgaagcg actcttgacc agctgactga ccaacagatt
     8641 ccattaacga ttgccacggc tactgtcaaa gagaatgttg ctttttattt tgatattttc
     8701 gatttagccc gctggtttga ctgggacaag attgtctatg acgacggctc tttccctggg
     8761 aaaccgcaac cggatatttt tcttaaagcg gcagacaaac tgagcttagc tccggctgat
     8821 tgtcttgtta tcgaagatgc ttactcagga ttacttgctg ccaaacgtgc ggggatcgga
     8881 acaattattg caattgatcc attcggtaaa aacaaaacag tatttgaaca ggcacagcta
     8941 aataaagatg gaaccatcaa agattttact ggtttttgga cagagcacct gtctgccaca
     9001 tccgttaatt cttaaacgat tcaactaact accaaagaaa agcaactgaa atgcacacct
     9061 ccttttataa aaaactgcca gtgaaacgac atgaaagata cactgctata tccttagtga
     9121 aatatagcag tgtatctttg actttttcat tcttttaacc atatgacgcg ttcatctcta
     9181 tctataatta acattaaaaa tagtgttttc acattatttc tcaaaccaat cttaaaaatc
     9241 atcaggagta cttttttgat atacttttat taatgctggg cgtaatggtt aaaagatttc
     9301 cactagttcc tctccaccaa gctgagttga aaaatgctta tctgtaaaca acatagacaa
     9361 agaggaaata aacgtttccg tatctttctc tgtcccaagg atattaatta attttcctac
     9421 taggtaagta tcattaaact taagatctgc cttcacatca aaagttttga taaaagcttt
     9481 tatcatgcgt acaattttgt ctttatcgcc ataattgatt tcaccactca tatcaatata
     9541 gtcactaatc cctgaagaaa taatggcaac tgcttcttct ttcgccttat cagataattc
     9601 atttaatgac tgggattgat tgatgacttc ttgagtttta tctgttacgt aatctctcat
     9661 tatcctatcg gcatactccg caaagtaatc ttgaaaaaat tcttctacag ggtaaccaac
     9721 ttcgttttcc caaccaggag aaatttcatt atcagtactt attaaatcta ttaaaaacca
     9781 acccgttctc
//
LOCUS       DAJSVP010000096         9397 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSVP010000096 DAJSVP010000000
VERSION     DAJSVP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.100"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            112..306
                     /locus_tag="ON449_002312"
     CDS             112..306
                     /locus_tag="ON449_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934854.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ON449_002313"
     CDS             575..874
                     /locus_tag="ON449_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934855.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ON449_002314"
     CDS             complement(971..1405)
                     /locus_tag="ON449_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934856.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ON449_002315"
     CDS             complement(1637..2347)
                     /locus_tag="ON449_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3934857.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ON449_002316"
     CDS             complement(2337..3197)
                     /locus_tag="ON449_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3934858.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ON449_002317"
     CDS             complement(3202..3840)
                     /locus_tag="ON449_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3934859.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ON449_002318"
     CDS             complement(3855..4154)
                     /locus_tag="ON449_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934860.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ON449_002319"
     CDS             complement(4197..5681)
                     /locus_tag="ON449_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3934861.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ON449_002320"
     CDS             complement(5701..6162)
                     /locus_tag="ON449_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3934862.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ON449_002321"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ON449_002321"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3934863.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ON449_002322"
     CDS             7465..8235
                     /locus_tag="ON449_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3934864.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ON449_002323"
     CDS             complement(8508..9335)
                     /locus_tag="ON449_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3934865.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2917 a         1929 c         1476 g         3075 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttcta
//
LOCUS       DAJSVP010000097         9330 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSVP010000097 DAJSVP010000000
VERSION     DAJSVP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.101"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..705)
                     /locus_tag="ON449_002324"
     CDS             complement(37..705)
                     /locus_tag="ON449_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3934866.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            839..1126
                     /locus_tag="ON449_002325"
     CDS             839..1126
                     /locus_tag="ON449_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934867.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1542..3080
                     /locus_tag="ON449_002326"
     CDS             1542..3080
                     /locus_tag="ON449_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3934868.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3077..4042
                     /locus_tag="ON449_002327"
     CDS             3077..4042
                     /locus_tag="ON449_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934869.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4029..5351
                     /locus_tag="ON449_002328"
     CDS             4029..5351
                     /locus_tag="ON449_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3934870.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5414..5518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5592..6335
                     /locus_tag="ON449_002329"
     CDS             5592..6335
                     /locus_tag="ON449_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3934871.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6350..7342
                     /locus_tag="ON449_002330"
     CDS             6350..7342
                     /locus_tag="ON449_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3934872.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7354..8091
                     /locus_tag="ON449_002331"
     CDS             7354..8091
                     /locus_tag="ON449_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3934873.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8081..8803
                     /locus_tag="ON449_002332"
     CDS             8081..8803
                     /locus_tag="ON449_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3934874.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8887..>9330)
                     /locus_tag="ON449_002333"
     CDS             complement(8887..>9330)
                     /locus_tag="ON449_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3934875.1"
                     /translation="SISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVV
                     QETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGR
                     YQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3058 a         1606 c         1971 g         2695 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      961 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
     1021 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1081 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1141 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
     1201 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
     1261 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
     1321 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
     1381 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
     1441 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
     1501 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
     1561 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
     1621 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
     1681 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
     1741 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
     1801 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
     1861 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
     1921 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
     1981 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
     2041 ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt
     2101 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     2161 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     2221 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     2281 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     2341 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     2401 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     2461 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     2521 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     2581 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     2641 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     2701 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     2761 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     2821 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     2881 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     2941 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     3001 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     3061 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     3121 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     3181 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     3241 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     3301 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     3361 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     3421 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     3481 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     3541 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     3601 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     3661 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     3721 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     3781 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     3841 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     3901 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     3961 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     4021 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     4081 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     4141 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     4201 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     4261 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     4321 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     4381 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     4441 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     4501 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     4561 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     4621 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     4681 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     4741 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     4801 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     4861 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     4921 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     4981 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     5041 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     5101 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     5161 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     5221 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     5281 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     5341 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     5401 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     5461 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     5521 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     5581 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     5641 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     5701 ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat
     5761 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     5821 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa
     5881 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     5941 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     6001 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     6061 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     6121 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     6181 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     6241 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     6301 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     6361 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     6421 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     6481 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     6541 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     6601 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     6661 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     6721 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     6781 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     6841 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     6901 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     6961 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     7021 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     7081 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     7141 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     7201 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     7261 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     7321 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     7381 gtagtcattg aaaacctgaa agtattagaa gacatccatt tcactatccc taaagatacc
     7441 tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     7501 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     7561 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     7621 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     7681 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     7741 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     7801 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     7861 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     7921 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     7981 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     8041 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     8101 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     8161 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     8221 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     8281 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     8341 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     8401 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     8461 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     8521 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     8581 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     8641 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga
     8701 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     8761 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     8821 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa
     8881 tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata
     8941 acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt
     9001 taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga
     9061 accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc
     9121 agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc
     9181 cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc
     9241 tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc
     9301 tgagtaaatc aggttaatat cagaaattga
//
LOCUS       DAJSVP010000098         9173 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSVP010000098 DAJSVP010000000
VERSION     DAJSVP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.102"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            50..2827
                     /locus_tag="ON449_002334"
     CDS             50..2827
                     /locus_tag="ON449_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3934876.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2909..3328
                     /locus_tag="ON449_002335"
     CDS             2909..3328
                     /locus_tag="ON449_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3934877.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3340..3810
                     /locus_tag="ON449_002336"
     CDS             3340..3810
                     /locus_tag="ON449_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3934878.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3827..4606
                     /locus_tag="ON449_002337"
     CDS             3827..4606
                     /locus_tag="ON449_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934879.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4593..5414
                     /locus_tag="ON449_002338"
     CDS             4593..5414
                     /locus_tag="ON449_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3934880.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5431..6480
                     /locus_tag="ON449_002339"
     CDS             5431..6480
                     /locus_tag="ON449_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3934881.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6493..7497
                     /locus_tag="ON449_002340"
     CDS             6493..7497
                     /locus_tag="ON449_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3934882.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8130..9149
                     /locus_tag="ON449_002341"
     CDS             8130..9149
                     /locus_tag="ON449_002341"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3934883.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3055 a         1537 c         1942 g         2639 t
ORIGIN      
        1 ttaaagattt tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt
       61 gttgaagaag gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca
      121 tatgaacgag gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac
      181 agtcagtcat tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg
      241 accagtgatt tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa
      301 tttttatttt tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga
      361 cttattctct tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa
      421 aacagctctt tactatccag atggcggatt accacttttg attacaggcg aaagtggcac
      481 aggtaaaagt tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga
      541 agattctgca ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt
      601 gactagcaat ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa
      661 aggggctttt gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac
      721 accagaaggt caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg
      781 agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat
      841 tactagtcat ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc
      901 acttaatcag cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga
      961 acaaagaaaa atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag
     1021 tcagaaatta tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa
     1081 agcatatgct gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa
     1141 agaactactt gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca
     1201 agaccagacc ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa
     1261 aagttttgag caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga
     1321 aggaaaatta aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag
     1381 acaagaaaaa cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca
     1441 aatggaaact gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta
     1501 tctctatcag cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat
     1561 caaagaatta gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg
     1621 aattctggaa ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt
     1681 actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat
     1741 gattgctgct cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt
     1801 acaagaggat ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc
     1861 ttcagaaatc cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt
     1921 agacatggga tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct
     1981 tttgattttg aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa
     2041 aaatgtatca ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg
     2101 actgctctat ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat
     2161 cggtacggcg gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt
     2221 gaaaatcatc ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt
     2281 ttccatatac gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga
     2341 ctatctttca ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt
     2401 ggacactact atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt
     2461 ttcattggaa aaagtactgg attccgtgac tattctagat acagagaagg tgatgggaga
     2521 aatcgatcat tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa
     2581 aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc
     2641 aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc
     2701 tattaaatct gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga
     2761 gattgcctat atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga
     2821 attttgaaca taagcaagtt gttgccacta attttggcat gcaacttgct ttttttagta
     2881 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
     2941 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
     3001 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
     3061 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
     3121 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
     3181 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
     3241 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
     3301 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
     3361 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
     3421 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
     3481 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
     3541 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
     3601 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
     3661 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
     3721 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
     3781 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
     3841 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
     3901 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
     3961 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
     4021 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
     4081 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
     4141 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
     4201 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
     4261 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
     4321 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
     4381 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
     4441 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
     4501 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
     4561 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
     4621 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
     4681 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
     4741 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
     4801 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
     4861 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
     4921 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
     4981 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
     5041 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
     5101 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
     5161 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
     5221 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
     5281 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
     5341 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
     5401 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
     5461 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
     5521 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
     5581 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
     5641 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
     5701 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
     5761 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
     5821 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
     5881 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
     5941 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
     6001 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
     6061 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
     6121 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
     6181 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
     6241 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
     6301 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
     6361 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
     6421 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
     6481 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
     6541 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
     6601 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
     6661 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
     6721 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
     6781 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
     6841 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
     6901 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
     6961 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
     7021 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
     7081 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
     7141 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
     7201 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
     7261 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
     7321 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
     7381 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
     7441 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
     7501 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
     7561 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
     7621 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
     7681 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
     7741 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
     7801 agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt
     7861 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
     7921 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
     7981 atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac
     8041 aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa
     8101 ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg
     8161 gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt
     8221 ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt
     8281 tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg
     8341 ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg
     8401 aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac
     8461 aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac
     8521 aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca
     8581 caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca
     8641 tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag
     8701 cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg
     8761 atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga
     8821 tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt
     8881 gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc
     8941 tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag
     9001 cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg
     9061 ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa
     9121 aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc gtc
//
LOCUS       DAJSVP010000099         8926 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSVP010000099 DAJSVP010000000
VERSION     DAJSVP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.103"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(181..918)
                     /locus_tag="ON449_002342"
     CDS             complement(181..918)
                     /locus_tag="ON449_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3934884.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(1071..2411)
                     /locus_tag="ON449_002343"
     CDS             complement(1071..2411)
                     /locus_tag="ON449_002343"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3934885.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(2648..4171)
                     /locus_tag="ON449_002344"
     CDS             complement(2648..4171)
                     /locus_tag="ON449_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3934886.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(4549..5160)
                     /locus_tag="ON449_002345"
     CDS             complement(4549..5160)
                     /locus_tag="ON449_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3934887.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(5283..5408)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5490..6206)
                     /locus_tag="ON449_002346"
     CDS             complement(5490..6206)
                     /locus_tag="ON449_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3934888.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(6212..6781)
                     /gene="scpB"
                     /locus_tag="ON449_002347"
     CDS             complement(6212..6781)
                     /gene="scpB"
                     /locus_tag="ON449_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3934889.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(6765..7562)
                     /locus_tag="ON449_002348"
     CDS             complement(6765..7562)
                     /locus_tag="ON449_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3934890.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(7532..7903)
                     /locus_tag="ON449_002349"
     CDS             complement(7532..7903)
                     /locus_tag="ON449_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3934891.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(7922..8809)
                     /gene="xerD"
                     /locus_tag="ON449_002350"
     CDS             complement(7922..8809)
                     /gene="xerD"
                     /locus_tag="ON449_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3934892.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         2547 a         1940 c         1484 g         2955 t
ORIGIN      
        1 agaaaataaa atataaatat aaacgaaact ttaaaaacgt acactaagtt ctctaaactt
       61 gaaaataagc cgggatctgt ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt
      121 catttgctat ctatagttaa ttgactagtt ttttcacgga ccctatagga cccatttata
      181 ttatttcaac tttttcagcc agttttccaa tgtattgacg atcacagttt gctggtctgc
      241 gttagaaatt tctgcctctc catctccttt ttgctcacca tagctgccaa aacctccatg
      301 atttccgcca ttgattgata catactctgt tctttccggt aaatactgct tagcttcttg
      361 ataagcttcc tgattcaata ctttatcttt tgtcgccgtg atcgataaaa caggaacagc
      421 tgattctgtc aatgttcctt ttttgtcggg ataacttgcc atgaagaata ccccttttaa
      481 atgtttgtct gaatgattct tattgacata acgacttgcc attacgccac ctaatgaatg
      541 gccaccgatc acataatcgg aatttggaaa atcttctttg accttatccg ctttgtttcc
      601 gcccatcact gctagatcaa gtggcatttt gacaatagcg actgaaaagc cgttttttgc
      661 taaatcttgc gcccaaatac tataactttc tggctctacg agagcaccag gataaaaaat
      721 aagtaatgga gactttccat gattcttttt ctctccttca aaaaataagt aatcttcttt
      781 gttttttcct tcttttacca acacttgcgc ttctgacgat ggttcgtacg tattttgctg
      841 tatatagtaa taaccggctc caccgagtaa tcctaaaatg atgactaagc caattagtat
      901 tcgtctaaat attttcactt tctcccacct tttctatctg agtgacccct ttttcttaat
      961 caagtatgat tataccttaa aaaaataaga gagaaatgca aactttccag catcaaataa
     1021 aaaaagacgg aatatccatt tgaatatccg ccctttttgt atgtttattt ttatttagca
     1081 cataaacgat acacagcttc tgcataaatt tccattgctt tatacatact acttaattcc
     1141 catcgttcat tcgcctggtg cataaagtca ggagtatcag ggaacatcgc accgaatgcc
     1201 acacactggt tcatcgtacg agcaaaagta gcgccgcctg atacaaacgg ttcagtcatg
     1261 tctccagtaa tatcgcgata tgtccccaat agatttttca ctaactcact gtctagtggc
     1321 acgtataatg aatcaaggaa gtcaaattct tcataagtca acccatattt ttctgctgtt
     1381 ttagttaatt tagctactaa atcatctttc ttgaatgtaa cagggattct catatctaca
     1441 ccgatttttg tttgttctgg tgtgatttcc aaacttgcaa agttcatcgt tagctcgccg
     1501 gattgttcat cttctgtttt gcccacgaca gcttcaccag ttgcattttc ttgaactaat
     1561 tttcctaaga aattgattgg gccaaaatca aatacttctg aaagagcaat tgccagacga
     1621 gaaatagcat tcactccttg tgctgcatct ttcgcgtgga tacttttacc aagaacgacg
     1681 atgccttcac cttgttcttc gaaatcgaat tcgtgttttt tcaatgcttc ttttacttca
     1741 gaaagctttt ctccagagta aggagctgca tcaggaacaa cattcaacgc tcctccagct
     1801 tttacagaaa actcgttcgt tcctggtcct gtcaagtaag cttgcaataa gcctttttct
     1861 gcataaatta aaggaaattc agcatctggc gcaaatcctt gcgtaattcc ttcttctttt
     1921 tcgttatatt tttctaaaca acgccataaa ttttcttcat ctgtaccaaa gataaaacga
     1981 atacgtgtct tgaattccac gccagcatca atcaaagctt tgacggcata cattgccgca
     2041 atagaaggac ctttgtcatc ttgggatccg cgaccaacta accagccgtc ttttatagtt
     2101 ggatcgaatg gttttgtttc ccaattattt tcgtcccctg ctggaacaac gtccatatga
     2161 cacaagatac cgaataattc atctccagag ccaatctctg aataaccgta atagccttct
     2221 ggatctttaa atgttctaaa tcctagcttt tcactgattt ccaatacttt atccaaagct
     2281 tcaatcactt ttttaccgaa gggatgtcct tgtcctgaat ctgcttcatc tagtacggaa
     2341 ggaattcgga tcaattcgct caatgattct aatgcttgtt catgatgttg ttctgtcaca
     2401 aattttttca tgatattatc ctcctaatat gttaaaaagg gtcatgtcaa attcctccga
     2461 cacaacctct tcttctcaaa taatgacgtt ttaagcctgc tccatcagca agcagaatgt
     2521 tcttaacaaa tgatgaaaga aaaaacactc actgaatgga gctattgact tgtccacgtg
     2581 cttatcgata gccgtttatc ggtttcttca ttcttatgat agaccttgta cctgtatttg
     2641 tctaggttta gaacattgct gctacaccta ggatcacaca gatggctaca aataatatcg
     2701 caattaattt accggtaaat ttccaccaca ctgaaacatc gaaacgagca attgctacag
     2761 cacccattac gatagcagat gttggtgtaa ttaagtttac taaaccagat gcagattgat
     2821 aagcagtgat tactaaatcc ttaccgacgc cggcaaattc acccataggt cccatgattc
     2881 ccattgttgc agcagctaag ccggaagttg aaggaatcaa gaatgacata gggatataga
     2941 aaataaatgt aataatgatg aatacacttg aagataatga acttaatcct tgttcgcccc
     3001 aatgtaagat ggtgtctgtg attaatccat tgttcataac cacttggata ccacgagcaa
     3061 cagctactac gattgctaca ccgatcaaat cagaagcacc agctaaaaat tcagagacaa
     3121 attcagactc tttcatgcca taagtgattg ctactacgat ggacattact aagaataaca
     3181 tagtaatttc tgggaagtac cagtcaccta atggaagcat gcttttacct agaaccgtac
     3241 ctagtaccgg aaggtttgtt aaccagtttg tcaatgattc aaaaatcgtg aaattcgcat
     3301 tcaaggttgt ccaaggaatc aaactgatga tcatgatacc aaaagtaata aagaacaata
     3361 cattaacatg tttttgtttt ttcgtgatta cttcttgacg accgattgct tcaatatcga
     3421 aatgcttcat atcttcttta cgttggttgt atactaatga ttttgttgga tctttttcga
     3481 tctttgatgc ataacgatac acatatacaa tagaaatagc taacaaaata acgaacatca
     3541 agaatcgaag accaatacct tcacccatcg aaatcccaac agcttgtgaa gctacgcctg
     3601 ttgcaaaagg atttaccgtt gaagctagac agcctacttg agaccctact aatacgattg
     3661 ctacagctac tactgtatca aagcctacag ccatcatgac tgggatcagt aaagcataga
     3721 atggaatcgt ttcttctgcc attccgtaag tagagccgcc taatgcaaat aacagcatca
     3781 atactggaat aagcattttt tcttttcctt tgaatttctt cacaactgaa gcaatcccgg
     3841 catctagtgc ccctgttttg ttgataatac ctaaaaatcc accaacaacc aaaatgaaga
     3901 aagagattgg gattgcagca ggtgtttctt tagttagctc ccccgctggt acacctagca
     3961 tcccattgat tggcgccatg aaaatatccc acagcccttg aggattttgt tgtactcgtt
     4021 catatgatcc agcgacgatg ttgcctgcat cgtctacact atagtgccca gctggtataa
     4081 accaagtaag tacagcgatt actgcaataa taataaataa gaccgtatac gctgaaggca
     4141 tttgaaattt tttctttttt gtttgttcca tcttcttctt cctttctttt ccgttagaga
     4201 caacaatttt ggctctaatg ttattttatc aaaaagagta tcagaacaaa aataataaca
     4261 cgcattttgt aaaacttttg ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc
     4321 agttttttaa agctttcttc tttttcattg tagcgtttcc ttttttttat aaacaaacat
     4381 tttcgtcttt taaaaggaga agaatttcac atttgcaaag aattgattga tttttatgca
     4441 taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta
     4501 tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc
     4561 tgtatgacgt tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg
     4621 cagcactaaa aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc
     4681 aatcagtaca tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa
     4741 cattaagtaa agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc
     4801 gatggttcct gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact
     4861 aaagaaataa taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc
     4921 taccaaatta tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc
     4981 tgttgcaact ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact
     5041 aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac
     5101 taatgcgatc gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat
     5161 ttgaaatcct cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga
     5221 aaaaactcta ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata
     5281 agctctatct tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat
     5341 ttttttgcaa aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga
     5401 agacgaacct tatataattt ttgtttacat attcattata catatctatt caaaaaacgc
     5461 aacatctaac aattataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact
     5521 tcttttttgt tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg
     5581 tatttttcac gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact
     5641 tgatgattac gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc
     5701 actgataaaa tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt
     5761 gtcaaaggtg caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta
     5821 tccacttcat gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc
     5881 aagatccccg aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc
     5941 ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct
     6001 ttaggtttgt acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact
     6061 tccacagtgt catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact
     6121 tttacacgtc ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg
     6181 gcaattactt tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga
     6241 atagatctgg tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt
     6301 cttctaatga tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg
     6361 gccgacctgg tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct
     6421 gtaccgctcc tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct
     6481 gtttataagc gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc
     6541 cttgcgcata tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc
     6601 tttccgtttc aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt
     6661 ctttcactaa acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat
     6721 tactgatccc ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca
     6781 tgttcggtat ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc
     6841 gcaatgatct gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca
     6901 cttttagaag gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata
     6961 cgctcttcga taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt
     7021 tccacttttt gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg
     7081 atttttttga cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc
     7141 ataggttctt ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct
     7201 gcatatttat atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct
     7261 tcccataatt cttcatcgtc tgtgccgaat tcttggacag gaagcaatgt cttgcttttg
     7321 attgccatca atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt
     7381 ttcattgtat ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat
     7441 atatcgattt ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca
     7501 tctaatttaa tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc
     7561 attttccaat gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt
     7621 cctgtaattc atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag
     7681 aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa
     7741 tgttggttga accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg
     7801 tatcaatctc cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc
     7861 ccatagcaat tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg
     7921 actaagccct tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg
     7981 tataaatctg agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat
     8041 cagctccatt ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt
     8101 ctttgtaaat cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag
     8161 aaagaccttt cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta
     8221 atggacgtgc ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa
     8281 tgatccgctc tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat
     8341 ctcccaattt caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca
     8401 agattgcacg atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt
     8461 ctacttctgt cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat
     8521 gttgcatcgg atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga
     8581 gacttgagat catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca
     8641 gaaaacggat gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta
     8701 gaaaatgcaa gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc
     8761 cccgttcaat attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc
     8821 tcatgcctct ctatcatatc agataccgct tcattctgat agtctttttc cataatctga
     8881 gcaaagaaaa agccgtctag acactcagac ggactggttt cttttt
//
LOCUS       DAJSVP010000100         8786 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSVP010000100 DAJSVP010000000
VERSION     DAJSVP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.104"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(266..2647)
                     /locus_tag="ON449_002351"
     CDS             complement(266..2647)
                     /locus_tag="ON449_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3934893.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2637..3284)
                     /gene="recU"
                     /locus_tag="ON449_002352"
     CDS             complement(2637..3284)
                     /gene="recU"
                     /locus_tag="ON449_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3934894.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3348..3887
                     /locus_tag="ON449_002353"
     CDS             3348..3887
                     /locus_tag="ON449_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3934895.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3977..4396
                     /gene="gpsB"
                     /locus_tag="ON449_002354"
     CDS             3977..4396
                     /gene="gpsB"
                     /locus_tag="ON449_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3934896.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4418..4785
                     /gene="rnpB"
                     /locus_tag="ON449_002355"
     ncRNA           4418..4785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON449_002355"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4994..6160
                     /locus_tag="ON449_002356"
     CDS             4994..6160
                     /locus_tag="ON449_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3934897.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6206..7702
                     /locus_tag="ON449_002357"
     CDS             6206..7702
                     /locus_tag="ON449_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3934898.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7783..8466
                     /locus_tag="ON449_002358"
     CDS             7783..8466
                     /locus_tag="ON449_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3934899.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2774 a         1585 c         1864 g         2563 t
ORIGIN      
        1 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
       61 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
      121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
      181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
      241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
      301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
      361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
      421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
      481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
      541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
      601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
      661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
      721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
      781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
      841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
      901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
      961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
     1021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
     1081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
     1141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
     1201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
     1261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
     1321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
     1381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
     1441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
     1501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
     1561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
     1621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
     1681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
     1741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
     1801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
     1861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
     1921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
     1981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
     2041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
     2101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
     2161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
     2221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
     2281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
     2341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
     2401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
     2461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
     2521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
     2581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
     2641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
     2701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
     2761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
     2821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
     2881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
     2941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
     3001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
     3061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
     3121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
     3181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
     3241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
     3301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
     3361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
     3421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
     3481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
     3541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
     3601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
     3661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
     3721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
     3781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
     3841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
     3901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
     3961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
     4021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
     4081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
     4141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
     4201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
     4261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
     4321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
     4381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
     4441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
     4501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
     4561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
     4621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
     4681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
     4741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
     4801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
     4861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
     4921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
     4981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
     5041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
     5101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
     5161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
     5221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
     5281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
     5341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
     5401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
     5461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
     5521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
     5581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
     5641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
     5701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
     5761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
     5821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
     5881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
     5941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
     6001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
     6061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
     6121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
     6181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
     6241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
     6301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
     6361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
     6421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
     6481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
     6541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
     6601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
     6661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
     6721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
     6781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
     6841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
     6901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
     6961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
     7021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
     7081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
     7141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
     7201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
     7261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
     7321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
     7381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
     7441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
     7501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
     7561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
     7621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
     7681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
     7741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
     7801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
     7861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
     7921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
     7981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
     8041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
     8101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
     8161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
     8221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
     8281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
     8341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
     8401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
     8461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
     8521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
     8581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
     8641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
     8701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
     8761 aaaaattgct gataaacgaa gatgga
//
LOCUS       DAJSVP010000101         8778 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSVP010000101 DAJSVP010000000
VERSION     DAJSVP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.105"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..341
                     /locus_tag="ON449_002359"
     CDS             <1..341
                     /locus_tag="ON449_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934900.1"
                     /translation="FSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPK
                     SISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKN
                     NNFYFYRNKL"
     gene            complement(467..1144)
                     /gene="nth"
                     /locus_tag="ON449_002360"
     CDS             complement(467..1144)
                     /gene="nth"
                     /locus_tag="ON449_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3934901.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1151..1849)
                     /locus_tag="ON449_002361"
     CDS             complement(1151..1849)
                     /locus_tag="ON449_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3934902.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2117..3301
                     /locus_tag="ON449_002362"
     CDS             2117..3301
                     /locus_tag="ON449_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR3934903.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3307..3750)
                     /locus_tag="ON449_002363"
     CDS             complement(3307..3750)
                     /locus_tag="ON449_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3934904.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3976..4263)
                     /locus_tag="ON449_002364"
     CDS             complement(3976..4263)
                     /locus_tag="ON449_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934905.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4434..5786)
                     /locus_tag="ON449_002365"
     CDS             complement(4434..5786)
                     /locus_tag="ON449_002365"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3934906.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6059..6505)
                     /locus_tag="ON449_002366"
     CDS             complement(6059..6505)
                     /locus_tag="ON449_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3934907.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ON449_002367"
     CDS             complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ON449_002367"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3934908.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7807..8694)
                     /locus_tag="ON449_002368"
     CDS             complement(7807..8694)
                     /locus_tag="ON449_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3934909.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2391 a         1790 c         1449 g         3148 t
ORIGIN      
        1 ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg
       61 caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc
      121 tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac
      181 ttatcatttt tattacacct ttagatagat acgtcactta tttatataat cctttggaat
      241 actggactta ttttataggt atattggaag gcattatttt ctacatcggg tattttccca
      301 ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta
      361 ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata
      421 ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt
      481 tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga catattgata
      541 gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa
      601 tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt
      661 ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca
      721 cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg
      781 tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc acttgcccat
      841 cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca
      901 aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg
      961 gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct
     1021 gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg agttctccat
     1081 gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta
     1141 acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt
     1201 gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt
     1261 ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt
     1321 aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg
     1381 gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg
     1441 ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt
     1501 tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt
     1561 aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc
     1621 catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca
     1681 tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta
     1741 gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat
     1801 tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc
     1861 cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt
     1921 tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt
     1981 ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga
     2041 atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc
     2101 ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt
     2161 ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac
     2221 gctgccttat tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt
     2281 ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg
     2341 gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg
     2401 tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg
     2461 agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa
     2521 agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg
     2581 gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt
     2641 cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc
     2701 agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat
     2761 ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct
     2821 tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg
     2881 tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg
     2941 attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct
     3001 cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg
     3061 gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc
     3121 caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc
     3181 tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct
     3241 tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata
     3301 gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt
     3361 gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg
     3421 atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat
     3481 ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt
     3541 tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt
     3601 aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc
     3661 aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta aatccagata
     3721 ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt
     3781 gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa
     3841 ccaagaaaaa aaggggttga caaagcttgt cataacccct ttttctgatt aaaagatagt
     3901 aaagagacag tcatcggtaa ttgtgatgat tttttgtcat cacttaagga tgagcggcct
     3961 tttcaaaaac tgtctttaaa gatcatcatc agtttctagc acatatggct gaccttcata
     4021 tgtgtactct gtattcaatg tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg
     4081 aaaacgatga gaaacattat ctttggcgtc atttgctggc atcgtgtaat attcctcttg
     4141 cgtttcattc aactgagaga gtacttgatc tgccaactca tcaatattga tcgttaattt
     4201 cttcgaagca cttttataat cttctaaaaa tgcatcttgt tggacaaaat attctttatt
     4261 catcttcttt ctcacctttc ttttcaaaat caaataaagc tgcttgttct tttatgtaac
     4321 agctttatct ttgctgctta ttgtgacaaa aaattcgaag aaagacaact aattgtactt
     4381 ttcacaaaaa aagacagagg gtcgtgacaa aattgtgtca caaccctttt tctttattgt
     4441 gcagtttcga tttcgcctag cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg
     4501 tcaaaatgtg gttggaagaa gaaatctgaa agtgcgagat cttctacagt cattttattt
     4561 tgaacagcta aagacatggt atttgctgat tgtgtaatgt cgtatttgga catgaactga
     4621 gcacctacga tacgattcgt tcctttttca tagactagct ccatcattac tttttcagta
     4681 gttggcatga actctggacg ataattatct tcaaatacag tcgcttggac ttctagatcg
     4741 ttcaaagttg cgctttcttt ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa
     4801 agtcctgaag ttccttgagt accacgatac gccattttat gatctgtcaa gttatttccc
     4861 acaagcaaac cttgacgaac ggcatttgtt gccaaaggaa tatagttttg cgtgccactt
     4921 ggattgtagt tgactactgc agagtctcca gctgcaaaaa tgtctggatt acttgtctgc
     4981 atgtattcat tcactttgat tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt
     5041 tttgtatttg gacggaagcc cacacataaa atgaccatat ctgtatcaaa tgtatctgtt
     5101 ggtgtagcta cttgtttcac ttttccttct tcatctgtga taaattcagt aacattttcg
     5161 cctagcgcaa gagtaacacc gcggtctgtc aattcttttt ctaagatatc tgtgaatggt
     5221 ttatctagat atttattcaa gatgcgatct aatccatcaa tcaatgtcac ttgttttcca
     5281 gattcgacaa aagcttctac taattcaata ccgatatatc caccaccaac aattactaca
     5341 cgttttgctt ctttcgcttg tgcaataatt tcatttgctt gattgtagtt tttgcatagg
     5401 agtacatttt tcgcatcgat tccactaatc ggtgggataa taggccatga accagttgtc
     5461 atcactaatt tatcataaga aaccgtttct gtttctcctg ttttcagatc ttttgccgtt
     5521 actgttttat tttctgtatc gacgtctgtt acatcgtgtt ccattttaac tgttgctcct
     5581 aatgaagata attcttcagg atttgagtag aacaagcctg ctgggtcctt tactactccg
     5641 ccgacataaa gtgcgatacc gcaagataag aaagaaatat tgtcatttct ttcatatact
     5701 gtcacttctg cttctggatg attttttaaa atacttttta cagctgcagt acctgcatga
     5761 gtacagccaa ttactactac tttcatttat ggtccctccg attaacattt tgtccttcga
     5821 caaaactact ataacagaaa taaaacggat acaacagttt aatgcttgtg aattcacaaa
     5881 ggtttgtttg tagtttcatt ccttttcaag cataagaacc caatatctac aagattctta
     5941 ttttaataat attcactttt atatctaaga ttgtgaataa aagacaaaaa aataaaaaag
     6001 ctagcgtttt ttttacacag ctagcttttt ttaggtataa cacttgtaga aactgttttt
     6061 acatttgcgc cttttttgct gactctttac aatccggaca gagtccatac aattccaacc
     6121 gatgttcatt aatctcaaaa tcagtcagtc tgcttgcagc catttctata tcttcaagcc
     6181 ctgggtaatg aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct
     6241 ttgaactgaa atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa
     6301 tcgaagtaaa taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat
     6361 gttcaagtgc tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt
     6421 ctaaaaccgc gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa
     6481 cctcttcgac taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat
     6541 actattttta cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt
     6601 taatgaaaaa agacgtaaaa cagtcgcgtt cactattcta cgtctctatt caaataattt
     6661 ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat
     6721 ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca
     6781 ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt
     6841 agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca
     6901 acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca
     6961 gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc
     7021 gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc
     7081 ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct
     7141 gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc
     7201 ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt
     7261 tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc
     7321 aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca
     7381 ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct
     7441 gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct
     7501 gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac
     7561 tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt
     7621 ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc
     7681 ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt
     7741 ctttgactag caaaaaaaag acaaagatat accatatatg gtaatttctt tgccttttta
     7801 tctaaatcaa acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc
     7861 atttttcgcc attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt
     7921 atcacctgac acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga
     7981 tgatgacgtt gaagtcgtcg tagtagtcgt tgttgttgta cttgtggttg ttgtagccga
     8041 acctgttgat ggcgtatcaa aacgggttaa attataagta ttaatcaatg agttcaattt
     8101 ggccccataa tttggatcgg tagcataacg acctgtcaag tacgctgttg catcataaaa
     8161 acttgttgta ttgcttttcc acacgccagc ataatgataa tcgcccgtgg aaaaactggt
     8221 tgaacgaaga acattcgcat ggtcttggaa ggattcccaa taagaagaat attttcggaa
     8281 tggctcattc atcgttaccc attgtccgtt tacatattct ttggttgcca tataaacgac
     8341 ctgtccgtta tagcttcctt tcacaccgaa tagattataa tttggcgcac tagctaatcc
     8401 ggaagtgcca tatgcacttt ccaacaacgc ttgagcgatc atgactgaag catacaagtc
     8461 attacttgca gcaacttctg acgcagacca gccgatttga tcgataaacg cttgaggatc
     8521 agcgactgtt tgagatcctt gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat
     8581 catcggccca actaaaagac tcgttccaat taaaggagca atcgcttttt tattgatcac
     8641 catttttctt ttaggtctat ggtgccgggc actgcgggtc ttcagctctt gcattcaaat
     8701 tcctcctcaa aataaaaaaa cttaataaaa agttctcttc ttaaaacaaa cttaattaat
     8761 taaattatac ttttaatt
//
LOCUS       DAJSVP010000102         8444 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSVP010000102 DAJSVP010000000
VERSION     DAJSVP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.106"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            124..2733
                     /gene="clpB"
                     /locus_tag="ON449_002369"
     CDS             124..2733
                     /gene="clpB"
                     /locus_tag="ON449_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3934910.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2884..3570)
                     /locus_tag="ON449_002370"
     CDS             complement(2884..3570)
                     /locus_tag="ON449_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934911.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3548..4042)
                     /locus_tag="ON449_002371"
     CDS             complement(3548..4042)
                     /locus_tag="ON449_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3934912.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4062..5282)
                     /locus_tag="ON449_002372"
     CDS             complement(4062..5282)
                     /locus_tag="ON449_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3934913.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5429..7264
                     /gene="lepA"
                     /locus_tag="ON449_002373"
     CDS             5429..7264
                     /gene="lepA"
                     /locus_tag="ON449_002373"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3934914.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7358..>8444)
                     /locus_tag="ON449_002374"
     CDS             complement(7358..>8444)
                     /locus_tag="ON449_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3934915.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2717 a         1615 c         1698 g         2414 t
ORIGIN      
        1 tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat
       61 aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc
      121 tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag
      181 attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta
      241 caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt
      301 gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa
      361 tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt
      421 gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta
      481 aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt
      541 aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa
      601 tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg
      661 gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa
      721 acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa
      781 gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt
      841 ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa
      901 agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc
      961 gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat
     1021 ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat
     1081 gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta
     1141 gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt
     1201 gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct
     1261 gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc
     1321 agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga
     1381 cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt
     1441 aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg
     1501 atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct
     1561 gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc
     1621 gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa
     1681 gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt
     1741 gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga
     1801 gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca
     1861 gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt
     1921 cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa
     1981 gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa
     2041 tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac
     2101 gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg
     2161 gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac
     2221 ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg
     2281 accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca
     2341 gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg
     2401 aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc
     2461 gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca
     2521 atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggtgca
     2581 agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt
     2641 gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat
     2701 ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa
     2761 aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt
     2821 agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt
     2881 ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct catctttttt
     2941 actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc
     3001 gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa
     3061 acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac
     3121 gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga
     3181 tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat
     3241 tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc
     3301 tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg
     3361 tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa
     3421 taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc
     3481 ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt
     3541 gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact
     3601 caatttctga agaaaccact tttccgttca atttgatcaa acctattgta tataagttca
     3661 ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc
     3721 cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca
     3781 caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta
     3841 aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag
     3901 cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga
     3961 gctctctgat ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg
     4021 gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag
     4081 taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct
     4141 aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat
     4201 gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc
     4261 tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg
     4321 atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca
     4381 tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc
     4441 aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa
     4501 tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct
     4561 tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg
     4621 ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc
     4681 caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg
     4741 gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt
     4801 ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct
     4861 ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg cttattcttg
     4921 cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct
     4981 ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact
     5041 tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact
     5101 ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg
     5161 ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga
     5221 tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc
     5281 attttttcat ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact
     5341 tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag
     5401 tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga
     5461 gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga
     5521 tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga
     5581 ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa
     5641 ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt
     5701 cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt
     5761 cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa
     5821 tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg
     5881 tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag
     5941 tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc
     6001 accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag
     6061 ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa
     6121 aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc
     6181 aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat
     6241 caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc
     6301 tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat
     6361 tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc
     6421 tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt
     6481 cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga
     6541 attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc
     6601 tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc
     6661 attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc
     6721 tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt
     6781 cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag
     6841 cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt
     6901 gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg
     6961 agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg
     7021 ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga
     7081 catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa
     7141 acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt
     7201 cgaagttccg caagaagcct tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa
     7261 atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc atgtttacat ggatttgcca
     7321 ccggtttgcc accggtggtt tttttcggtg gcaaaaatca agaattcttt ctaagatttt
     7381 ccatgatatc gaccgtttca tttctcatct tatctgttac atgcgagtaa gtatccatcg
     7441 taattgaaat tctgctgtgg cccaatcgtt cagagatttc tttcattttt gcgccatttt
     7501 cgagaagaag tgtagcatga gtatgtctga gagaatggaa gttaaaagag agggagagtg
     7561 cattcgatat cctcctagta ttccatttca ctacacttgg cgtaactagc tcaccgtctt
     7621 ccttcgtaca tactgcattt gaatcaatgt agagctttcc atacttcatt cgattttcta
     7681 attgttgttt cttatgtttt ttcagaattg ctagcaaggt ttgtccaata aaaatcgttc
     7741 gattggaact gcttgtcttt ggcgtaccat atacccatgc gccgtcattc tttaccattt
     7801 gtttctctac agtaattgtt ccatttgaaa aatcgacatc atcccacgtc agaccacaaa
     7861 cttcgccaac gcgcattccc gtataaaatc caatattcaa aggaatatag aaaggatgtc
     7921 cttcaggagt aatttctagc atatgatcaa agtcctcaag agaaatgatt tttagatctt
     7981 ttttagtcgt tggtcgtgct tcatattttg gtatttttac atacagcata ggattttgct
     8041 tgattaatcc ccaaggataa accgccatat tcagcgcatt cttaaggaca gagtgagtaa
     8101 tagtcattgt tttcttcgag taaccctttt taaattcagc attgatgaaa ttttgtaaaa
     8161 gagcagggga gagatccgta agttttttct ttcctaaata accgtttata tgatttttga
     8221 tggtaaatcg gtagttttca taggtattgt attttagatt tagtttaacg tattcctcca
     8281 tccaaaaatc aaggtattgt tttactcgag tatccgtacc taaaaagtat tgtcctgttt
     8341 cgtcaatatc tgataaaact tttcgtaaag cagcttcggc ctctggtcgg gtgtctccgc
     8401 caactttctc cacttttttt cttgagccat catcattgat atct
//
LOCUS       DAJSVP010000103         7817 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSVP010000103 DAJSVP010000000
VERSION     DAJSVP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.107"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..710
                     /locus_tag="ON449_002375"
     CDS             <1..710
                     /locus_tag="ON449_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3934916.1"
                     /translation="KQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGI
                     VNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKD
                     TIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVK
                     TPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELT
                     RQMKRDAGAVGGTLYAT"
     gene            700..1440
                     /locus_tag="ON449_002376"
     CDS             700..1440
                     /locus_tag="ON449_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934917.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1437..4199
                     /locus_tag="ON449_002377"
     CDS             1437..4199
                     /locus_tag="ON449_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3934918.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4212..5120
                     /locus_tag="ON449_002378"
     CDS             4212..5120
                     /locus_tag="ON449_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCR3934919.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5120..5740
                     /locus_tag="ON449_002379"
     CDS             5120..5740
                     /locus_tag="ON449_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934920.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5744..6193
                     /locus_tag="ON449_002380"
     CDS             5744..6193
                     /locus_tag="ON449_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934921.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            6193..6687
                     /locus_tag="ON449_002381"
     CDS             6193..6687
                     /locus_tag="ON449_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934922.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6701..7132
                     /locus_tag="ON449_002382"
     CDS             6701..7132
                     /locus_tag="ON449_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934923.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7134..7271
                     /locus_tag="ON449_002383"
     CDS             7134..7271
                     /locus_tag="ON449_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3934924.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            7308..7601
                     /locus_tag="ON449_002384"
     CDS             7308..7601
                     /locus_tag="ON449_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934925.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            7598..>7817
                     /locus_tag="ON449_002385"
     CDS             7598..>7817
                     /locus_tag="ON449_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3934926.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT         2827 a         1085 c         1646 g         2259 t
ORIGIN      
        1 ttaaacagtt tttcaaagat acttggaaaa atattaaaga tacggcatac aatacgtgga
       61 tttctataaa aaataccatg attaatacat ggaataacat taaggactct ttctggaata
      121 ttgttactgg aattgttaat tctgctgaaa atgcatggac aaatctaaaa aatggtgttt
      181 caaatgctat taatcgggta aaagaaatct ttaattcgtt aagggaagtc aatttatttg
      241 aaattggcaa gaacatcatt gatggactta taaatggtgt aaaagaaaag tggaatgctt
      301 tgaaagatac tatcaaaggt attgcaggta atatcaaaaa ttggattaaa ggagctttag
      361 atattcattc tccatcaaga tggatgcgtg atatggttgg taaaaatatt gttcaaggta
      421 ttatcgttgg tattgataaa gagcaaagta agttagatca aaccatgaca aatttagtaa
      481 aaacaccgtc tgttcaacct gtagtaaatg gaataagtca cctaccaatt acacaaacaa
      541 agcaaaatat atctgctagt gaaacaaaag aaattcattt acatttaaat gtttatggag
      601 atttacctga ttcaatggtt cggaagatta ccacaaaaat taaaacagaa ttgacaagac
      661 aaatgaaacg agatgctggt gcagtaggag gaacattata tgcaacttaa gagaggacaa
      721 ttttttatta atcaacatta ttcttctgaa tttaatgtgt atattcaaaa caggcctgct
      781 tctgtttcag ctagtcgagt aattgaattg agagagcgag aaggaaatga ttctatcatt
      841 attgataaag gctattacag aaatgtgact agaaagattg agtgttacta taaagcacct
      901 tcaattgatg tggtgcagga gtgggaagat cgaatcactg aatggttaga tatgtcgtct
      961 tatagtgatt ttattcttta ctatgatgaa caatatattt atcaagctgt agtaatagaa
     1021 ggacctgaat ttaaaggaac aagaaaaaca ggaaatattg tcccttttga atttacagtg
     1081 agcattcgac catttaagga aaattacaat ggtagattta ctattcgaca aacagaaact
     1141 tttgaaattt ataacccaga gaagtattct tcaaaaccgc ttattaaatt gagtggttct
     1201 ggagatgctt ctttttatat taataaggac aaatatgatt tgaaatcgtt ggacagagaa
     1261 ttatttatag attctaaatt agaagaggct taccgaaaat tagatggtaa tttagaacat
     1321 caggatcaag tcactttatt tttagatttt ccatttttat atcctggaaa aaatgaaatt
     1381 aaatggacga aaaatattca ttcatttgaa ataatgccta ggtggtggag aaaagtatga
     1441 aaccaagaat atatagtcct actgaaacag atttctcaac gaatggctta ggaattttaa
     1501 aggattgtac aagatgtgaa atatatgaag tagctaatgg aaaatatgaa ttagagttgg
     1561 attatccttt aggaactaga tttgatgaat attttgaaaa tgactatcaa ataaaagcaa
     1621 agccaaacga tcaagaagag tatcatatct tttttattga tgataaagat atagatactt
     1681 ttttaaatac agtaactatt tatgctcaga gtcgtacaaa tcgacttgga agacgggtag
     1741 tcactcttgc ggaagtagac tctaaaactg gtcaagaagc gatatcaatt attgaaacca
     1801 aaatggataa aaaatctgac atacgacttt attctgatat tacggccgtt tctagcacaa
     1861 tctttgaagc gagaaatgtt ttaaattgta ttgctggtga acaaggatca ttgcttcaat
     1921 attggggtgg agaaattaaa cgtgaaccat ttaagctttc tttgttaaag cgaagaggtc
     1981 gcaataatgt tggaacgatt cggtatggaa aagatatgtc tggtttaaag gtcaaattag
     2041 attggacagg tataaaaaca agacttattc catatgctga tcctcaaagt gaggtaggta
     2101 cgactaatcg aatttatggt tcgccagtag atagtgccta tattaataac tatcctgatg
     2161 tgtatacaga gcatgttcag tttacagaag aacaaggagt aaaagacatt aagagcttaa
     2221 ataaaatagc taaaaattac tttaaaacga tcaatcctgg ctgtgataag cctaaagttt
     2281 ctattacggt tgaatttgat aagttgactg atagtgaaga agcgaaagaa tttgcgaaga
     2341 ttagaaatta tggtctgttt gatacattta aaatatatca taaaaaatat aatatttatc
     2401 ttgaatcaaa ggttagtgga ctacaatacg attctttatc tgaaaaaact ttgaaattag
     2461 aagcaggaga cattcaagtt gctttttatc agcaacaagc tgtaactatt caggataaat
     2521 taaaagatta tgcaacgaat aattatatga gtgattttaa tgattatgtt tcttcaatga
     2581 ttacaggtca aggcagtgca ggaggatatg taagtttatg gccaaaagaa aaaccttcca
     2641 acatctttat tatggataat ccagatttag aaaaggcaag agaagtacta agaatgaata
     2701 aaaatgggat tgctttttct aagaaaggat ggcgggggcc ttttacttca gcttggacat
     2761 tagatagtat atttaatgcc aattttatac aaactggatt aatcaaagca gatatctttc
     2821 aaaattcttt taacaagact ggagatgtat tgaaattagt taatggacta ctccaaattt
     2881 ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg aatggaattt tggaattcta
     2941 agggttcaat tggaacgatt ggaacaactg attctgctgg taatcctttt cctggggctt
     3001 ctactccaac ccctattgaa gataattctt tagttattcg tacaaatgga gacggcaaat
     3061 atattttgat ttctcctaaa gtaggaaaag gattagtttt attaggaaat ggtaaagcaa
     3121 tttattttgg agacttagat gtacaaggaa aacttacagt taaaggaaaa gaaataattc
     3181 ctggacaagg tggtggtcca tcgggaggtg gagaatctac tggtgggtat cctagtgaat
     3241 taaaaacaga tgcagaaaag agagcttgga gaatttacga tattttgtgt aataacggat
     3301 ttacgaaaca atctgcatgt ggaatattgg ggaacattca acaagaaaca ggaggaactt
     3361 ttgatcctga tactgttcaa ataggtggac cagcatacgg attagttcag tgggatggtt
     3421 cttcatatcc tcttgttggt ccagcaacat gggatggaaa agtttatgtt caaaacttat
     3481 ttaatgctgc aggtattaaa gaaccaataa cgagcttaga tgcacaagtt cgattgctta
     3541 tttggacatt tacaaatgga caatggatgg gcgtagtaca acctacgacg gttgatggat
     3601 ttaaggcctg tactgatcca agacaagcag catatgcttt tgaacgaaac tatgaacgtc
     3661 cggcagcgac acatcctgaa cgtcaagatt atgcagttaa ctggtataac aaatttaaag
     3721 atttaaaacc aggaggagct actggagaag caggactaaa acatttagag tctttgattg
     3781 gacaacgaat tggcaatggt cagtgttatg gcttgtctgc agaatattca ggatatctgg
     3841 gtggttgtgg catgggtgct ggaacaaaat atggtttaac tcatgtgata ggaaatactt
     3901 ctgcagcatc tgatattggt attgcctatg attggtctgc tgttggttgg aaagtgattc
     3961 aaaaccctag atatgatcaa ttagtagttg gtgcaattat taattgggca agaggtggac
     4021 aagtgggttc atggtttgca gacggaactt atggacatac tggcgttatc cgaggcttag
     4081 ctaatggtcg tatgcaaact tatgaacaaa acacagagtt aggtatgatt tgtggaaagt
     4141 tagatcgcca gtattatagt gcaagtgcaa tttcttccat tgtcatacca ccaaaatagg
     4201 gaggtgattt aatggcaaag tggaatgtca tattaagtac aacagaaccc tataattacg
     4261 tggggatgat tcaagttcga caaggcaata aaaacactga agttatggaa gctacaattg
     4321 tcgaaaatgg tcttccatat gatttatcag aatgtaaggt atattttgaa tcagttgttg
     4381 gcggaaaata tccagtccaa ttagaaacaa gaattgtgga tgctaaaaaa gggaaaatca
     4441 aatatatttt tgataaatat tccatgcagt gtttgcatcg acaaacagcc aatttcatta
     4501 tatttaaagg agaagacttg attggaacaa ctcaagactt ctcctatttt gtcatcaatg
     4561 ctgtttcaaa aacagaagga gaaatgggtt cttattggca atcaattgaa gatttaattg
     4621 ctgatatgac agattttatt aatgaaaata aaggcgattt tacggactgg atgaatgaaa
     4681 ggaaagaaga atttgatcgc tggagagaag aacaggagaa tagtttccaa gattggagag
     4741 aaggacaaga atcagattat ttgtcatggt ttgaatcaat caaggatatt ttgaagtcta
     4801 tcgatccagg tggagtaatg ttagccgaat taatggatgc acgtgtagat cttcaaggtg
     4861 tacgccatga atcgatttct gaacgtttcc tatccgatct aaattatttg tatcaaaaaa
     4921 tgaaagcagc acttttcacg attgaatacg gtgaaattga agtgactgat attcttcaag
     4981 acgatctctt ttcagataat cacgaagtcg aaaaagttgg aactgtagaa ttcccgattg
     5041 aagaaggagc cttgatcatc gcaaccgttg atgatccaaa gcaaaatgtg ttcactcttg
     5101 agaaagtcgg ggtgatctaa tggctaaaac taaacgaatg atggaaacgg atgaaaaaac
     5161 aggtgtacaa cgccaattct ttccaatcac acatgcttcc gcagttcttg ggttagaaga
     5221 aataatagca ggagaagcaa cagttttatc tgttaatgga aaaattggag ccgtcgtcat
     5281 tactaaagaa gatttgggat tagagaatgt tctcacagaa ttaccctatg caagtgaaga
     5341 agatgacggc attatcacgg cagaaatgta tcaaaaaatt ttaaacagtg gagaaggtga
     5401 ctacgtgttg ccagttgcca ctatcgaccg tttgggtggc ataaaagttg gtgaattatt
     5461 gacgattgat gagacaggga aagtctctgc agtcagacaa tctgatgtca atttttcgct
     5521 ggagttaaaa gaaaaactcg attcactgaa aaattatact gctggagaaa acatcactat
     5581 tgatgaagat ggaacaatta gttctacagg tggtagtggg actggtggtg tcaatcagtc
     5641 atatgtagat caaaaattcc aagaagctgt aaatcaagca gaaaattaca caaacgaaag
     5701 aattccaaat tttacttttg aaaaaattgg ggaggtatag agaatgacag atatcgtaaa
     5761 aataaaacaa agcaatgtac aggtttatcc acaaactcat tggaatgcta tagaaggcaa
     5821 accaacaacg gtgaagggag ataaaggcga tcctggccaa gcagcaacaa ttaccatagg
     5881 aaccgtttct agtggttcaa ccgcttctgt tacgaatgta ggaacatcat ctgcagctag
     5941 atttaatttt gttttaccaa agggagataa aggtgatcct ggaataaatg ccacaactac
     6001 agctgtagct acaactacag caaatgggtt gatgtcgtcc acagataaaa ctaagttgga
     6061 tggaattgca gctggagcac agaaaaatcc aggtaatgct acaactacga cagcaggttt
     6121 aatgtcagca accgataaag tgaaacttga tggattagcc aatattacat ttgagaaagt
     6181 ggggacggtt taatggctga tattgttcaa ttaaaagaag acggagttgc taaatactta
     6241 aaaacgcatg taaaagctat tgatggtaaa gaagcattgg tgcagacaga tggagaccaa
     6301 gccattgcag gacataagaa tttttcaggt tctgtaacaa tcaataataa acgtgttttg
     6361 acgactgatg ataacagata tgaagtggta aatctggttg tcactaacgg taatacaggg
     6421 acagcaaagc tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga
     6481 aaaagtagtg gcgggaatga ttcggttatt ttaactgttc cagaaggcta tcggacacca
     6541 attagttttg aacaactggt tgggtcaata gaccgttcta ctttgaacag tgctcaacta
     6601 tctattggcg cagacggagc cattaaatgg agaagaaatt caagttatgg atcagcttat
     6661 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
     6721 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
     6781 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
     6841 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
     6901 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
     6961 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
     7021 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
     7081 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
     7141 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
     7201 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
     7261 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
     7321 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     7381 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
     7441 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     7501 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
     7561 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
     7621 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     7681 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     7741 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     7801 cgaagaagga ggaaaca
//
LOCUS       DAJSVP010000104         7720 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSVP010000104 DAJSVP010000000
VERSION     DAJSVP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.108"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            45..1232
                     /locus_tag="ON449_002386"
     CDS             45..1232
                     /locus_tag="ON449_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCR3934927.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1213..2517
                     /locus_tag="ON449_002387"
     CDS             1213..2517
                     /locus_tag="ON449_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3934928.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2543..3556
                     /gene="citC"
                     /locus_tag="ON449_002388"
     CDS             2543..3556
                     /gene="citC"
                     /locus_tag="ON449_002388"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR3934929.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3560..3853
                     /gene="citD"
                     /locus_tag="ON449_002389"
     CDS             3560..3853
                     /gene="citD"
                     /locus_tag="ON449_002389"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR3934930.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3858..4763
                     /gene="citE"
                     /locus_tag="ON449_002390"
     CDS             3858..4763
                     /gene="citE"
                     /locus_tag="ON449_002390"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR3934931.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4756..6315
                     /gene="citF"
                     /locus_tag="ON449_002391"
     CDS             4756..6315
                     /gene="citF"
                     /locus_tag="ON449_002391"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR3934932.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6312..6869
                     /gene="citX"
                     /locus_tag="ON449_002392"
     CDS             6312..6869
                     /gene="citX"
                     /locus_tag="ON449_002392"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR3934933.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6862..>7720
                     /gene="citG"
                     /locus_tag="ON449_002393"
     CDS             6862..>7720
                     /gene="citG"
                     /locus_tag="ON449_002393"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR3934934.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWL"
BASE COUNT         2643 a         1224 c         1609 g         2244 t
ORIGIN      
        1 ttaaagtggt tttgtaatta aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt
       61 tagaaatcca taaaaaaaat gtaggtgttt tacggatgga aacaacagaa gccgtgcaag
      121 atgatcaaga tttagcaaaa atgtacaccc caggtgtagc agaactaagt ttaatgattg
      181 agaaaaatca tgctttggca agagaattaa caattagtgg taaattagtg gccgttatta
      241 cagacgggtc ggcggtatta ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg
      301 tcgaagggaa agcgctgctt tataaaaatt tagcaggtgt caatgcgatt ccattagcca
      361 tcgaacaaaa atcagtagac gagattgtac aaacaattgt caatttgcaa aatagttttg
      421 cagggatcca tttagaagat attgcagcac cgaagtgttt tgagattgaa gaaaaattac
      481 aagaaaaact ttctattcct gtgtatcatg acgatcaaga aggaacagcg atagttgttt
      541 tagctggatt gatcaatgct gctaagatcc aaaggaaaac attaactgaa cttcgagttt
      601 tgatcaacgg catgggagca tctggggttg caacagctag actattaatc gctgctggta
      661 tcaaaaatct aacattggtg gataaacaag ggagattaaa gaagcaagat agtacattaa
      721 ataaatatca aagagagttg ttggatcata tacatgatac aaataataat gcaagcgttt
      781 tggaagatgc aatcgttgga caagatgttt ttatcggtct ttcaatagga aatgttttaa
      841 cgcctgaaat gattggaaaa atgaatgata atccgattat ttttgcttta gccaatccag
      901 taccagagat cacacccgat ttagcaaaag aatctggtgc aaagctaatc gctacaggtt
      961 cttcgaaata tcctaaccaa gtaaacaata ttttagtatt tcccggattg ttcaagggac
     1021 tattactatc gaaagcgaat aaagtcgatc tttcattaca acttgaagta gccaaacaat
     1081 tagctgctaa ggtcgcagag cctacggtag aaaaattcat ccctaatgtt tttgatgaag
     1141 gagtcgcaaa cgtcgtttca caggcagttt tggattattt acaaaaacta aaagaaaacg
     1201 aggataatat acatgctgaa aacattaaat agaattaaaa ttggttttat tcctttatct
     1261 ctttatatca ttttggtact cgcgtatctt gcaatgacga taagcaatat cgttccagaa
     1321 aatgatatgt tagtttcttt gggtattatg actgtttttg cttatttatt agaagaaatt
     1381 ggtaaaacta ttcctatttt aaaagattta ggtggtaaag tattagttgt tacatttttg
     1441 cctgcatttc tagtctataa acattggcta cctcaaagta ctgtaactgt ggtttctgat
     1501 tttatgaata ataacaattt cttgatgttc tttatcacac tactagttgt cggcagtatt
     1561 gcttcaatga atcggcaaac attggttaaa gcaacttcac gtattatcat tattttaatt
     1621 ttatgcgatg tgattggttc tttgatagga acaggtgtgg gaatactcct tgggctatct
     1681 gcttggaatg cgtatttctt tatcgttgcg ccgattatgg ctggtggtgt tggggaagga
     1741 gcgatgccat tatcgattgg ttatgcagca atattatctg gtatgggaca aaatgatatc
     1801 tttgggaaga ttctaccgtg tgtgatgttg ggaagtttag tcgcagttct actgtctggt
     1861 atgctcaaaa aaattggaga gaaataccca caattaaatg gtcagggtaa tttgatcgat
     1921 ggggatgatg aacatttaga gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa
     1981 acattgttag caggcgtggt agcaataacg atttattttg ttgctgtcta tgttgagcac
     2041 ttattaaaat cactccttgg cttttctatt ccttcacctg tgatcttatt agtctctctt
     2101 atgggggcta aaatgcttgg ttttatccct aaaaatatcg aagaaggtgg aaattcgttg
     2161 tatggattca ctgttcgtgg gatcacacct cccttacttt ttggcgtagg ggttgcaaaa
     2221 acagattggt tagagctgat cgaagtattt aaaaatcctg caacgatagt aacaatcatt
     2281 gtgacggtag tagctattgt cgctacagct tttatcgccg caaaattttt aaaaatgtac
     2341 ccagttgata ctgccatcgc agtttcatgt tgtagcggac aaggcggtac aggtgcttta
     2401 gctatcttag ctgcgggtga tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga
     2461 ctagggggtg ctatgaccgt aacgttcgct atctttttaa tgggtctgct atcataagaa
     2521 cataaagaaa ggacgtgacc atgtgtttga tcaaacagat atccaacgat tatttttaaa
     2581 aaataactat atcaaagacc aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa
     2641 gcaaatcgaa gattttcaac atttagatca aacattgggt atataccata aagaaaagtt
     2701 agtaggaaca gtatcttatc aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata
     2761 taaagatggt ggacatacat ttaatgtact agtctctgca ataattcagg caatgagtca
     2821 acaagagatt tttcattata ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt
     2881 tttaggtttt gaaacaatcg tatctacccc aaaaatagct gtattagaaa tgggagatag
     2941 ttctataaaa cagtacctag agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat
     3001 tgtcatgaat gcgaacccat ttacagaagg gcattattat ttagtaaaac aagctgccaa
     3061 taacaacgat tgggtatatg tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa
     3121 ggaccgactc aaattagttc aacaaggatt acaaaaactg aaaaatgtca ttgttctatc
     3181 aggtggcgag tatattatta gccctgcaac gtttcccacc tattttttac gagaagatga
     3241 tgaaatagca aaggagcaaa tggtcgtaga tgctacttta ttcaaagaaa gaattgccac
     3301 ttatttagat atctctgcta gatatgtggg agaagaacca ctttccccaa tgactcgttc
     3361 atataacgac atactggaaa cgattttacc ccctgaaata aaagttcatg tccttgcaag
     3421 gaagaaaacg gaacaacacc aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc
     3481 tgggcaattg gaaaaaataa aatatatggt tccagaaaca acctatcaat acttaaaaaa
     3541 taaaagggag cgttaagtaa tggaaataaa taaatcagcg attgctggaa cattagagtc
     3601 aagtgatgtc caaatcatga tttcaccgaa tacagaagga atagaaatcg atttagacag
     3661 tcaagtgatt gagcaatttg gaaaacaaat caaaaaagtt atctatgaca cactagcagt
     3721 ttatcagatt aaaaacgtaa aactcaaggt agtagataaa ggggcattag attgcgttat
     3781 tgttgcacga ttaaaaacag cgatccatcg ttccttaggt atccaacacc aagaaacacc
     3841 gtgggaggtt taattagatg atgaaagaac gtttgagaag aacaatgatg tttgttccag
     3901 gaaataatcc agcgatgatc aaagatgcag gtatttatgg tgctgactcg attatgttcg
     3961 acctggaaga tgcagtgtct atggcagaaa aagacagcgc aagaatgctt gtggctgagg
     4021 cattgaaaac agtggattat ggtgacacag aattaattgt tcgaataaat gacctgcact
     4081 cagaatttgg acgtgcagac atttttgcaa tggttaaagc cggcgcgcat gtattacgtt
     4141 tacctaaaac agaaacctct gaagatataa cagaagtaga aaaagtgatt atagaagcag
     4201 aaaactatca taatgttccg atagggcgaa cattgatgat ggcagccatt gaaggtgctt
     4261 ctggtgtatt gaacgcacct gcgatcgcca aagcatctga tcgattgatt ggaatcgcca
     4321 ttggggcaga agactatgtg acaaatatga aaaccaaacg atatccagat ggaaacgaat
     4381 tgttttttgc tcgcagtatg atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg
     4441 acactgttta ctctgatgtg gatgatatgg aaggattttt aaacgaagta cgtttaatcc
     4501 atcaattagg ttttgatgga aaatcttgta tcaatccacg gcaaattcca gtagtcaata
     4561 ctgtttatca gccgtcaatc aaagaaatca aacgatcact agacatcatc tcggcaatcg
     4621 aagaagcaaa agaaaaaggt tctggcgtaa tttctttaaa tggaaaaatg atcgataaac
     4681 caattgtaga acgtgcacaa cggacattga tgttagcaaa agcatcacga ttgattgatg
     4741 aggagggaaa tatccatgca taataaacaa agacgtagca ttccatcaag tgttttaaca
     4801 aagtataaaa aagaattcca aagtgtttca ttatcgccac aaacgattca acgttgtgca
     4861 ccaaaagtaa cgactgtaac taatcagaca aataaattga tgcactcgat caaagaagct
     4921 atagaaaaat cgggcttaaa agatggaatg acgatttcat ttcatcatca tttgagagaa
     4981 ggtgattttg tttttaacca agtgatggcc gtgatccgtg aaatgggcat caaagaattg
     5041 accttagcac caagttcttt aacgaacact atgaatgaga tggttattga atgcatcaag
     5101 gacggtacag taactcatat cacaagcagt gggatgcgcg gcagcttagg tgaatttatt
     5161 tctcatggag gaatggacaa tcctgtgatt ttacgttctc atggtaatcg tccacgtgca
     5221 attgaaagtg gtgaattgaa aatcgatgtt gcatttctag gagtaccaac atctgatgat
     5281 tatggaaatg ctaatgcgac tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa
     5341 gtagatgcac aatatgcaga taaagtcatt cttctaactg atcatttagt tccttaccct
     5401 aatacgccca ttagtattcc gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt
     5461 ggtgatccga ataaaatcgg tgctggtgct acacggttta ctaaaaaccc aaaagaatta
     5521 aaaattgctg aaatggtcaa agatgtcatt gttcactcac cttatttcaa agatggcttt
     5581 tcttttcaaa caggaacggg tggtgcagct ttagcagtaa cgcgttactt agctagtgaa
     5641 atggaagaaa aacagatcaa agcttctttt gcattaggtg gcatcactaa accaacggtt
     5701 gatttattgg aaaaaggtct tgttgaaaaa gtgttagatg tccaagactt tgacaaagga
     5761 gcagctgaag gaatggcgcg gcttgagaac caacaggaga tcgatgcttc cttttatgct
     5821 gatccaagta ataaaggagc ggttgttaac caattagatg tcgctatttt atctgcatta
     5881 gaaattgaca caaacttcaa tgtaaatgtt atgactggtt cagatggtgt gctaagaggt
     5941 gcgattggtg gacatcaaga tgcagcaacg gctaaactta ctatcatctc agcgccgtta
     6001 gtacgtggcc gaatacctac agtagtaaat gatgttacta cattgatcac accagggaaa
     6061 agtatcgatg tattagtcac agaagtaggg gttgctatca atccccaacg taaagatcta
     6121 atagctattt ttgaaagaat tccacaaatt cctgttttta caatagaaga attacagcaa
     6181 aaagcagaaa aaatagtagg catcccggaa cctttgcaat tcacagatcg gatagttgcc
     6241 tatgtagaat accgagatgg ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa
     6301 gaagagagat catgatgttt gatactggag aaattcaaga aatcgaggat attttgatcc
     6361 ataaagaaca acgtgctaac ttgcaaagca acctgctttc caagtattca aatagttcat
     6421 tgatggtctt tacattgaat attcctgggc cgatcaaaaa taatccaatg attgagcagg
     6481 tatttcgtca gggacagcag tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga
     6541 cgtacacaaa agaaatcaat ttgccaagtg gattggactg gtacgtaata gtaacagaag
     6601 cacctttgaa tctcaaaaaa tggttggtca cgttagaaga gcaagacgca ttaggtagac
     6661 tttttgattt agatgtcttg ttcaaaaaag gagagcagat aacaattgtc agtcgccaag
     6721 ctcttaatca tggggaacgt ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac
     6781 gtaatcgtac acatactgtg aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt
     6841 ccttaaaagg agcattgaaa catgcatgat aatattcaaa aaatagcaca agaagcacaa
     6901 ttagcgatgc tttatgaagt tacatgtatc aataaaccag gattggttga tccagtagac
     6961 cccgggtctc atcaagatat ggacattttt acttttctac aaagtagtgt agttttgact
     7021 ccctattttg aagaatttat ccaaacagga ttggaccgac aacatcaacc aattgaagaa
     7081 acctttatgg cgattcgaca gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca
     7141 aatggtataa atacacataa aggtgctatc ttttcattgg ggatttttct tgctatttgt
     7201 ggccggttaa ctatttggaa agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa
     7261 aaaatgacga aaaatctact taacgatttt gggaagattg atcaaacaaa accaaaagca
     7321 tggacttggg gagaatatct atttgttaac catggattga cagggatacg aggggaagcc
     7381 aaaagaggtt atccttgtgt ttttgaccga ggattgcctt attatcaagc atatcaaggg
     7441 tcgcaacaag ataagatgat agacacttta ctttttctat caatggaagt agaagatacc
     7501 aatttgatca aacgaagtaa taatagacat gtttttgaag agtatcaatt acttattcgt
     7561 ccttattttg aattaggggg tgtaaaaacc atacaaggta aaaaatattt ggattaccta
     7621 aatcagcaat ttaaaaagaa aaattggagt attggtggaa gtgcagactt attgatcctt
     7681 acaatctttt tagataaaat catggacaaa ggatggttat
//
LOCUS       DAJSVP010000105         6970 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSVP010000105 DAJSVP010000000
VERSION     DAJSVP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.109"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(136..594)
                     /locus_tag="ON449_002394"
     CDS             complement(136..594)
                     /locus_tag="ON449_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3934935.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(607..1938)
                     /locus_tag="ON449_002395"
     CDS             complement(607..1938)
                     /locus_tag="ON449_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3934936.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1949..3391)
                     /locus_tag="ON449_002396"
     CDS             complement(1949..3391)
                     /locus_tag="ON449_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3934937.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3378..4142)
                     /locus_tag="ON449_002397"
     CDS             complement(3378..4142)
                     /locus_tag="ON449_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3934938.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4246..5121)
                     /locus_tag="ON449_002398"
     CDS             complement(4246..5121)
                     /locus_tag="ON449_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3934939.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5376..5460)
                     /locus_tag="ON449_002399"
     tRNA            complement(5376..5460)
                     /locus_tag="ON449_002399"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5424..5426),aa:Leu,seq:caa)
     gene            complement(5478..5549)
                     /locus_tag="ON449_002400"
     tRNA            complement(5478..5549)
                     /locus_tag="ON449_002400"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5515..5517),aa:Gln,seq:ttg)
     gene            complement(5559..5631)
                     /locus_tag="ON449_002401"
     tRNA            complement(5559..5631)
                     /locus_tag="ON449_002401"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5596..5598),aa:His,seq:gtg)
     gene            complement(5693..5777)
                     /locus_tag="ON449_002402"
     tRNA            complement(5693..5777)
                     /locus_tag="ON449_002402"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5741..5743),aa:Leu,seq:caa)
     gene            complement(5798..5868)
                     /locus_tag="ON449_002403"
     tRNA            complement(5798..5868)
                     /locus_tag="ON449_002403"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Cys,seq:gca)
     gene            complement(5914..5985)
                     /locus_tag="ON449_002404"
     tRNA            complement(5914..5985)
                     /locus_tag="ON449_002404"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5951..5953),aa:Gln,seq:ttg)
     gene            complement(5997..6069)
                     /locus_tag="ON449_002405"
     tRNA            complement(5997..6069)
                     /locus_tag="ON449_002405"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6034..6036),aa:His,seq:gtg)
     gene            complement(6072..6145)
                     /locus_tag="ON449_002406"
     tRNA            complement(6072..6145)
                     /locus_tag="ON449_002406"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6111..6113),aa:Trp,seq:cca)
     gene            complement(6152..6232)
                     /locus_tag="ON449_002407"
     tRNA            complement(6152..6232)
                     /locus_tag="ON449_002407"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6196..6198),aa:Tyr,seq:gta)
     gene            complement(6234..6309)
                     /locus_tag="ON449_002408"
     tRNA            complement(6234..6309)
                     /locus_tag="ON449_002408"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6274..6276),aa:Phe,seq:gaa)
     gene            complement(6345..6417)
                     /locus_tag="ON449_002409"
     tRNA            complement(6345..6417)
                     /locus_tag="ON449_002409"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6382..6384),aa:Asp,seq:gtc)
     gene            complement(6427..6499)
                     /locus_tag="ON449_002410"
     tRNA            complement(6427..6499)
                     /locus_tag="ON449_002410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6464..6466),aa:Val,seq:tac)
     gene            complement(6515..6586)
                     /locus_tag="ON449_002411"
     tRNA            complement(6515..6586)
                     /locus_tag="ON449_002411"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6551..6553),aa:Glu,seq:ttc)
     gene            complement(6601..6690)
                     /locus_tag="ON449_002412"
     tRNA            complement(6601..6690)
                     /locus_tag="ON449_002412"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6654..6656),aa:Ser,seq:gga)
     gene            complement(6730..6803)
                     /locus_tag="ON449_002413"
     tRNA            complement(6730..6803)
                     /locus_tag="ON449_002413"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6767..6769),aa:Asn,seq:gtt)
     gene            complement(6813..6928)
                     /gene="rrf"
                     /locus_tag="ON449_002414"
     rRNA            complement(6813..6928)
                     /gene="rrf"
                     /locus_tag="ON449_002414"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2098 a         1545 c         1142 g         2185 t
ORIGIN      
        1 cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa atctagtgat
       61 tgaaacatat catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac
      121 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
      181 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      241 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      301 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      361 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      421 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      481 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      541 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      601 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      661 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      721 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      781 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      841 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      901 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      961 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
     1021 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
     1081 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1141 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1201 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1261 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1321 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1381 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1441 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1501 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1561 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1621 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1681 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1741 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1801 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1861 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1921 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1981 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     2041 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     2101 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2161 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2221 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2281 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2341 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2401 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2461 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2521 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2581 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2641 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2701 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2761 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2821 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2881 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2941 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     3001 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     3061 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3121 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3181 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3241 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3301 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3361 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3421 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3481 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3541 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3601 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3661 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3721 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3781 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3841 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3901 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3961 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     4021 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     4081 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4141 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4201 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4261 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4321 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4381 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4441 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4501 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4561 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4621 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4681 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4741 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4801 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4861 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4921 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4981 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     5041 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     5101 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5161 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5221 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5281 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5341 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5401 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5461 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5521 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
     5581 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
     5641 agatgttgtt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
     5701 gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc
     5761 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
     5821 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
     5881 tcaatctata aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc
     5941 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
     6001 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
     6061 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
     6121 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
     6181 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
     6241 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
     6301 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
     6361 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
     6421 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
     6481 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
     6541 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
     6601 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
     6661 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
     6721 aaatgagaac tccggcagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct
     6781 actaccaact gagctatgcc ggaataacat cagcgtggcg acgtcctact ctcacaaggg
     6841 gcaacccctc actacaatcg gcgctaagaa gcttaacttc tgtgttcggc atggttacag
     6901 gtgtatcctt ctcgctatcg ccaccacact gtggtgttat cttttattga gtaaattttg
     6961 ttcactcaaa
//
LOCUS       DAJSVP010000106         6961 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSVP010000106 DAJSVP010000000
VERSION     DAJSVP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.110"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            361..642
                     /locus_tag="ON449_002415"
     CDS             361..642
                     /locus_tag="ON449_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934940.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="ON449_002416"
     CDS             644..1060
                     /locus_tag="ON449_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934941.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="ON449_002417"
     CDS             1682..2836
                     /locus_tag="ON449_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCR3934942.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="ON449_002418"
     CDS             2830..3252
                     /locus_tag="ON449_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934943.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="ON449_002419"
     CDS             4422..5390
                     /locus_tag="ON449_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3934944.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="ON449_002420"
     CDS             5407..5931
                     /locus_tag="ON449_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3934945.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            6107..6511
                     /gene="tnpA"
                     /locus_tag="ON449_002421"
     CDS             6107..6511
                     /gene="tnpA"
                     /locus_tag="ON449_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR3934946.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            6528..>6961
                     /locus_tag="ON449_002422"
     CDS             6528..>6961
                     /locus_tag="ON449_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934947.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT         2624 a         1181 c         1140 g         2016 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa
     5941 tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg
     6001 tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt
     6061 tgtcccatgt tacaatcagt ctatacaaga aatgagttga tataacatgg aattagatac
     6121 aaataatcat tcagtatttc ttctttatta ccaccttgtt ttagtaacga aatatcgtag
     6181 acaagtgatt gatgatgaaa tatctgatta tgccaaaact acttttgaaa gaatttcaga
     6241 atcgtatcac attactttag tggagtggaa tcatgataaa gaccacgttc atattatgtt
     6301 caaagctcaa cctaaaacag aattgacaaa attcatcaat gcctataaaa gtgccagctc
     6361 aagattgata aaacgtgact ttccaagagt caaacaattt ctttggaaag aaatgttctg
     6421 gtctaaaagt ttttgtcttc tgacaactgg tggcgcacca attgatgtga ttaagaagta
     6481 tatccaaaat caaggaaaca atcataaata gaaagtaggt gaactttatg gaacaactaa
     6541 aagcatacaa attcagaatc tatccaacag aggaacaaga aatcttcttt gccaaatctt
     6601 ttggttgtgt ccgtaaggtc tacaatctaa tgcttgatga ccgaaaaaag gcgtatgaag
     6661 aagttaaaaa tgattcttct aaaaaaatga cttttccgac accagctaaa tacaagaaag
     6721 aatttccgtt tttgaaagaa attgatagcc ttgctttagc caatgcccaa cttaatttag
     6781 ataaagcata caagaatttt tttcgggata aatccgttgg atttccacgt ttcaaaagta
     6841 agaaaaatcc tgttcagagt tatacaacga ataatcaaaa tggtacagtt gctttgattg
     6901 atagtaaatt catcaaagtt cctaaattga agtcattagt aagaattaag cttcatagac
     6961 a
//
LOCUS       DAJSVP010000107         6905 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSVP010000107 DAJSVP010000000
VERSION     DAJSVP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.111"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(718..3867)
                     /locus_tag="ON449_002423"
     CDS             complement(718..3867)
                     /locus_tag="ON449_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3934948.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="ON449_002424"
     CDS             complement(3880..5079)
                     /locus_tag="ON449_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3934949.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="ON449_002425"
     CDS             complement(5069..6664)
                     /locus_tag="ON449_002425"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3934950.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1943 a         1414 c         1115 g         2433 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcat
//
LOCUS       DAJSVP010000108         6847 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSVP010000108 DAJSVP010000000
VERSION     DAJSVP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.112"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..463
                     /locus_tag="ON449_002426"
     CDS             11..463
                     /locus_tag="ON449_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934951.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            477..1166
                     /locus_tag="ON449_002427"
     CDS             477..1166
                     /locus_tag="ON449_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934952.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1291..3642
                     /locus_tag="ON449_002428"
     CDS             1291..3642
                     /locus_tag="ON449_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3934953.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3722..4117
                     /locus_tag="ON449_002429"
     CDS             3722..4117
                     /locus_tag="ON449_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934954.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4442..5059
                     /locus_tag="ON449_002430"
     CDS             4442..5059
                     /locus_tag="ON449_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3934955.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5100..5399
                     /locus_tag="ON449_002431"
     CDS             5100..5399
                     /locus_tag="ON449_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3934956.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5433..5600
                     /locus_tag="ON449_002432"
     CDS             5433..5600
                     /locus_tag="ON449_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3934957.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5645..6208)
                     /locus_tag="ON449_002433"
     CDS             complement(5645..6208)
                     /locus_tag="ON449_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3934958.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6236..6799)
                     /locus_tag="ON449_002434"
                     /pseudo
     CDS             complement(6236..6799)
                     /locus_tag="ON449_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2307 a          989 c         1366 g         2185 t
ORIGIN      
        1 ggtgagaatc gtgaaaaaaa cgatactaag tattttttca gtatttacaa tattaattgg
       61 gggctatttt cttttgaatt tagattctgt taattcaaac gctcaaataa aagatcaaag
      121 agtagctgaa cataataata aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga
      181 tgaagaattg tataaagaat ttggtgattt atccggggaa cagttagaag aaagtattcc
      241 aggaattagt gactcagata aaattgttgt cttgtctgct ggtgggcaat taatgggaga
      301 tggcaaatcc gaattagtta gttataaaca aaatcacaga cagtttattg atgttaatac
      361 tgattccaac gctatttcga ttaaggatct aaaagagctt ttaaatagta aaagaaatga
      421 attaaagcag ataaaagaaa ctaattcagt ggaattagag taaaggaaga ttcattatga
      481 aaaagatttt tggtgtttct gttgggctac ttatgatgtt aacatttgga aatgcagtat
      541 atgctgctga gaaagaagtt atttcagatc cagagcagta cgcctcacga tactttgctg
      601 attttgaaga agaagataaa gtacttgcat tgccagaagg tggtttttta aaaggggaag
      661 ctaaaattgt atctatggat aatccagata taattcaagg ttcatataat tcagaaactg
      721 atcctgcttc agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg
      781 atattgaaac tactccaatt atgcctcgag gagcatttcc acccgatgtt ggtactagcg
      841 gacaccttct tgtactagca ggaggaggaa gctataaatc acagccattt agtggtgcgg
      901 gatggcgttt tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt
      961 ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat
     1021 ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa
     1081 acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta
     1141 cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt
     1201 tattattttt agttgttgac aaacactaaa atataaaata taatcaacac ataaacgaac
     1261 gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa
     1321 tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga
     1381 acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact
     1441 aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct
     1501 aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt
     1561 aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta
     1621 aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg
     1681 aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt
     1741 aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg
     1801 ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct
     1861 gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa
     1921 acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta
     1981 tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt
     2041 ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat
     2101 gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc
     2161 gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata
     2221 aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag
     2281 tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc
     2341 tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt
     2401 aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt
     2461 gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag
     2521 ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat
     2581 gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg
     2641 actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt
     2701 gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg
     2761 ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc
     2821 aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct
     2881 ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt
     2941 ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa
     3001 tataggaata aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact
     3061 ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc
     3121 gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc
     3181 tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt
     3241 acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat
     3301 gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac
     3361 tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct
     3421 tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc
     3481 ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca
     3541 gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa
     3601 aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa
     3661 acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga
     3721 catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc
     3781 tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac ttttaataac
     3841 tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc
     3901 tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt
     3961 aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga
     4021 aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa
     4081 ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag
     4141 ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa
     4201 actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta
     4261 gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg
     4321 acatgccttg attttctttg tttatgtatg gcataataac cacataatac aatttgaaac
     4381 gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac
     4441 gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact
     4501 gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg
     4561 caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt
     4621 actctatcta caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg
     4681 gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga
     4741 tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca
     4801 attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg
     4861 cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc
     4921 tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat
     4981 ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac
     5041 cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa
     5101 tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc
     5161 aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa
     5221 caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa
     5281 aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag
     5341 aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat
     5401 cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg
     5461 gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg
     5521 aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat
     5581 acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat
     5641 atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt
     5701 cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa
     5761 ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc
     5821 ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct
     5881 cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga
     5941 gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt
     6001 tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta
     6061 gtgatgtaac gatgacgcaa tcaccttcac ggatataatt tagtaatttt tgaaactcag
     6121 gacgctcagt atctttacca ctggcttttt cttggaataa tttatcaatg ttaagtcctt
     6181 cggttctgtt gcgaagtttg aaaatcaaac gcgccctctc tgaactccaa atatcttagg
     6241 ctgttattcc cattaatacc ttgatttcag tagacaccga aaagccgaag agcgttccat
     6301 ttcttcggtt ctttttatat attcctcgaa tggtctccat gcccttaatc gtggaagagg
     6361 ctgtacggag actttgataa aatttatttc gtcgtttaat aggtcgatgg tcttgttcta
     6421 ttaaattgtt aagatacttc acagttcggt gctctgtctt agtatataaa cccacactct
     6481 gtaactttct aaaggcggag ccaagagaag gtgctttatc ggtcacaatt gctttcggct
     6541 caccaaactg tttatggagt cgttttaaga aagcataggc tgcttgcgta tcccgtttct
     6601 ttcgtaacca gatatctaag gttaagccgt ccgcatcaat tgcacgataa agataatgcc
     6661 aacgtccctt aattttgata taggtttcgt ccattttcca tgaatagaag gattgtctat
     6721 ttttcttctt ccaaagataa tagaggactt tgctgtactc ttgcacccaa cgataaatcg
     6781 tagtatgaca aacatttatt ctatggatag gctacaatta actagacaga aaaattaagg
     6841 tgtgtag
//
LOCUS       DAJSVP010000109         6821 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSVP010000109 DAJSVP010000000
VERSION     DAJSVP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.113"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..1189
                     /locus_tag="ON449_002435"
     CDS             35..1189
                     /locus_tag="ON449_002435"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3934959.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1211..1963)
                     /locus_tag="ON449_002436"
     CDS             complement(1211..1963)
                     /locus_tag="ON449_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3934960.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2327..4993)
                     /locus_tag="ON449_002437"
     CDS             complement(2327..4993)
                     /locus_tag="ON449_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3934961.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5334..6785
                     /gene="cls"
                     /locus_tag="ON449_002438"
     CDS             5334..6785
                     /gene="cls"
                     /locus_tag="ON449_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3934962.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2012 a         1403 c         1242 g         2164 t
ORIGIN      
        1 gtaagttttt tatcataagt aaagaggtgt tgaaatgaaa atagggattg atcgtctttc
       61 cttttttatt cctaatttat atttagatat gacagagttg gcagaaagcc gtggggatga
      121 tcctgcaaaa taccatatag ggattggtca agaccaaatg gcagtcaatc gtgcaaatga
      181 agacatcatt acactaggag caaatgctgc cagcaaaatc gtaacagaaa aagaccgtga
      241 gctaatcgac atggtcatcg tcggaacaga atccgggatt gatcattcaa aagcgagtgc
      301 ggtaattatc caccatttac tgaaaatcca atcttttgct cgttcttttg aagtaaaaga
      361 ggcttgctac ggtggcaccg cagctttgca catggcgaaa gaatatgtca aaaatcatcc
      421 agaacgaaaa gtactagtca tagcaagtga tattgctcgt tacggcttgg caagcggtgg
      481 tgaagtgacg caaggtgtcg gtgctgttgc gatgatgatc actcaaaacc cgcgtatttt
      541 atcgattgaa gacgacagcg tatttctgac agaagatatc tatgatttct ggcgtccaga
      601 ttatagcgaa tttcctgttg ttgatggtcc tttatctaat tctacgtata tcgaatcatt
      661 ccaaaaagtt tggaatcgac ataaagaatt gtcgggtcga ggactcgaag attatcaggc
      721 gattgctttc catattccgt atactaagat gggaaaaaag gcattgcaaa gcgtattaga
      781 ccaaacagac gaagaaaatc aggaacgtct tatggctcgc tatgaagaaa gcatccgtta
      841 cagccgacga atcggtaatc tttacactgg ttcattatac ctggggctaa cttctctact
      901 ggaaaattcg aaatcactac agccaggaga tcgcatcggt ctattcagct acggttctgg
      961 tgcagtaagt gaatttttta caggttatct ggaagaaaac tatcaagaat atctcttcgc
     1021 acaatctcat caagagatgc tagattctcg aacacggatc actgttgacg aatatgaaac
     1081 gattttcagt gagacgcttc ctgaacacgg agaatgtgca gaatatactt cagatgtacc
     1141 tttttcgatt acaaagatcg aaaatgatat tcgttactac aaaatataat aaaatcataa
     1201 aaagaaccct tcacgagtcg tgaaaggttc tttttttatc tgcggaccaa aggagcagtc
     1261 tataggtcag cttagtcgta ctaaggattt ctcgtttttc actgaatagt ttgcgaatgt
     1321 acctggtcac aacaggatct tttgcgtacg cgtcatagta tagcttcatc aattcttcag
     1381 taattgattc ctgtgtaaaa taagtaaaat cttttttctt cactttgaat ccttcatcaa
     1441 ttaagtatgt ttcaaattga tccattacaa ctggcgatgt attaggatct tcttctatca
     1501 ttcctagttg cttttcgata ggataaaaaa catcatcgat tgattccact actctgatta
     1561 tcacgcacca tttgcttgac tttttcatca atttttctaa ttgaataaga tgtaccaatg
     1621 ctggatttgc tgctgtaaaa accatttcat atgtctcatt tgattggata aattcttcta
     1681 acgactgttt gataaaggaa acatttggca actcattttt ttctgcttct ttttttgcat
     1741 tttctagcat tcctgaagaa aaatcgatca aatcatagcg attaatttct tttgctaagg
     1801 aaagaaatcc gccagatcct ccagcaacgt ctgctgctgt ttcagcaggc aggactttct
     1861 ccgttttcag ataatttttt acatcttcag caatagttac ttgcgattct tgctggattt
     1921 cataatattc ttcagcaaat tcatcccatt ctttttcatt catttgattt cctctaattt
     1981 tttgttatat gttttcactt ttcggaataa atcattctcc tcttttatct attctatctc
     2041 ttttgatgaa taataaaatg attggctttc cgtctatcat aaataaaaaa agggactgtg
     2101 accaaagtcg tgtcaaagtc cctctttccg aataaacagt gtccaatcag atgaccctca
     2161 gtattagctc aaaatgctga ttttacaact tcttccttaa acggtgttct cctgccaact
     2221 cttcggtcac aagtcacaat attcaacaaa catgagaagc tgtttgttga aattgcacct
     2281 tgtgactcag aattaaacgg caggctcaca acctcgttga ttcgttttat ttatttcctt
     2341 gtactcgtac aatgatcaat ttagtgattt ccatcaaaac aattgtcaca agtgcgattc
     2401 ccatacatgt aaagacttct gtcaatccaa atgctgatgg gatagagaag acttctttca
     2461 gtcctgggat cattgttaag cagtaaagcg cgctacatac aactacagct agtaagacac
     2521 ttctgttaga gaagaaccct gcttggatag ctgtttgtgt gtttgaacgt gctgggaatg
     2581 tttgcaatgt ccgacaccaa atcaatgttg aaaatgacat ggctacaccg aattcaggtg
     2641 aatgctgcat gccaagccat tgagcaaaga taacagcaat cccaatcagc acaccacgat
     2701 acgttacaga aatcaatgtt ttgtccgtaa agattcctga atttggatct cttggcgggc
     2761 ggttcatcgt agcaggttca gctttttctg tacctaatgc aattgctggc agtgaatcat
     2821 tgactaaatt gatgaacaac agttgtaatg ctgtaaatgg attggaccag tcagcaacta
     2881 aggcaaagat gatcgcaatg atcgcaccta aatttcctgc aaatagataa gaaatagctt
     2941 tcttgatatt atcaaacaca ttacgtccta cttctactgc gctgacaata gaagcaaagt
     3001 tatcatccgt caataccatc gctgctgcat cttttgccac atctgtaccc gttcccatag
     3061 caattccgat gtctgcttgt ttcaatgctg gtgcgtcatt tacaccatca cctgtcatag
     3121 ctgagatatg acctttattt tgccaagcac gaacgatacg aattttattc tcaggtgaaa
     3181 cacgtgcata aacagtgact ttttctaaaa cttcgtttag ttgatcttct gtcaattcat
     3241 ctagttccgt cccggtcaaa gccaggtttc cttcttcaaa aatcccgatt tgttttgcga
     3301 tagcaacagc tgttgttttg tggtcaccag taatcatgat tgttttgatt cctgcacttc
     3361 ttgcttctgt taccgcctga gtcacttcag cacgtggagg atcgatcata gcaagcaaac
     3421 caacaaggat caaatcgtct tcatcttcaa atgataaatc aaaattatca agtggacggt
     3481 aagcaaaagc tagtacacgt aatgcgcgtg ttgaaaaagc ttcattttgc tcttggaatt
     3541 ttttcatcaa ttcatccgtc atcggaacga cttcaccatt tagcaatact tttttactgc
     3601 gttggaagac gatatctggt ccgcctttaa caaacaagta atttttgtta tcaattgtgt
     3661 gtccagtaga cattaatttt cgatctgagt caaaaggaag ttctgcttgt cttgggtagt
     3721 tgtttcggat ttcctgataa ggctggttta atttttcact aaagtcgata aacgctactt
     3781 ccgtagggtc gcccaattta gtaccgtctt cactgatcga tgcatcattc gctaaaacac
     3841 tgatttcgat caagcgacgt tcgtcagctg tccattttga agggtcattt gtgaaatccc
     3901 cagtatcgcc attacctaaa aagtagtcaa caactgtcat tttgttttgt gtcaatgtac
     3961 cagtcttatc tgtacagatg atacttgtcg aaccaagtgt ttcaactgct ggaagcttcc
     4021 gaataatggc atgacgtttt gccattttat tcgttcccaa agataacacg attgttacga
     4081 tagactgtaa tgcttctgga atagcagcta cggctacggc tacggcaaac atcactgcgc
     4141 tgacgatatc tgcagtcata tctcctgtac cttctccaag ataaatacga aacagttgga
     4201 tacctaaaat caataaagac aatgccaaaa tagccaaact tagtttttta ctgaatttat
     4261 ccagacctcg ttgtaacgga gtgacttgct cagttgttga ttccaaaagg ccagcaactt
     4321 gtccgatctc cgtattattc ccagttgaag tcactaagaa tttaccgcgt ccgtaagtta
     4381 cgatcgttcc actgaatacc atatttgtac gatctccgat tggtaccgta cttgtgatat
     4441 ccgttacttc tttttctgca gaagtcgact ctcctgtcag cataccttca tctactttta
     4501 atgatcccgc atctagtaaa cgtccatcag caggtacata atcgcctgcg tctaacaaca
     4561 cgatatctcc taccacaagt tcgtttgccg gaatcgtttt ttccttacca tcacgaatca
     4621 ctcgagcatc gggcgctgaa aggtttttca acgcttctaa agaaccttct gctttttttg
     4681 tttggacaac actgattata gaattcaaca tcaatactgc aaaaataact aagaattcga
     4741 tgtagtcacc catgaccatc tgaatcagtg cgacaatcag caatacgata accattgcat
     4801 ctttgaaagt ttctaagaac aatttccaag ttggcttctt cggcttttct tttagctggt
     4861 tcaagccatc tctttctaat ttttccttcg cttgcgtggt gctcaatcca tcggttgagc
     4921 tctgcgtttg ctgcatcaac gagtcgatcg tttctttata gaccgcttgt tgtgcttgtt
     4981 ccttgttttc caaatttcat cccccatttt ttgatcttgg ccctagtcct gtctgaaaaa
     5041 aaagagacct aagacaaata cgcatttgtc ttaggtctca ccatttcata caataccaga
     5101 aaaccatttg gttaacttac tgttgatatt atcgcaacaa acgttgccgg ttactcccct
     5161 aaggagatat tcaactgtac taatatccta ccttattaac ctacaaaaag caactacttt
     5221 agcccgattt acctacattt tcatgaaagg agtgaaaagt atattcacct tggtaggctt
     5281 ctttcttcta tcatgctata ctaatgttat tgtatgtaag gaggaacatt tttgtggtat
     5341 ctagtattat aaccgccctt tatctattaa atgcacttat tgctttggtg gctattttga
     5401 tcaaaccccg agatgtagca gccatttggg catggctctt agtattgttt gcccttcctg
     5461 gggtgggctt tgtattatat ttatttttcg gacgtggatt aacggataag aaaaaattct
     5521 atctccgaca aagtgacttg aaagaattag aaaactttca gtcttttaga ggagatacca
     5581 ttgaacatta cgatcctgac atgggcgata aagacaaaca gcaatttgtt gacttcttct
     5641 cttcattaaa tcgtatgccg ctgacaagaa tgaattctgt cactcttctc acagacggac
     5701 aagagaaatt ggattcactg cttcaagatc taaaaaaagc caaacattcg atccatatcg
     5761 aatattacgc atttgtgaca gataatatcg gccagcaagt cttacatgtt ttagaagaaa
     5821 aagccgcaga aggcgtggaa gttcgaatat tatatgatgc atttggctct catggcacaa
     5881 aagcaaaaga tttcaatcgt ctaatcaaaa atggtggaca tgtccataca tttgttacct
     5941 cacaaagggc attacttcgt ttccgattga attaccatga tcaccgaaaa atcgttgtga
     6001 tcgatggaaa gattagttat accggtggtt tcaatattgc caatcaatat gtaaatacaa
     6061 caaaaaaatt cggctattgg cgcgatacgc atatacggat tttcggtgcc gcttcttctt
     6121 tgctccagct tcgcttctta acagactgga acgtctcggt acctgaagaa aaaaaggtcg
     6181 gctatcattt gaattatttc tttaaaaaag cagatcgaga tgaatctaag cttgctgata
     6241 catccatcca gcttgtttca agcggaccga ataacgaaag ggaacaaatc aagctttcat
     6301 ttatcaaatt gattacttct gctaaaaaac gtgtttggat acagacacct taccttgttc
     6361 ctgatgaaag tgtcattgct gctttaaaaa tcgcaactgc ctctggtgta gatgtgaaaa
     6421 tcatgattcc caacaaaccg gatcatcctt ttatttatcg agcaacacaa tattatgctc
     6481 ggcagctgat caaggaaaat gtacaaatcc ttgtctatga gaacggcttc ctccatgcaa
     6541 aaacattgat aatggatgat gaaatctgca tggtaggttc agcaaatcaa gatattcgta
     6601 gctaccgatt gaattttgaa acaagtgctg tcatttacga tcctgagttt ttagaagaac
     6661 ttgctactca gttcaaagaa gatgagacac attgttcatc catgacaact gaaacagtca
     6721 aggaaatgtc taactggcta ttattcaagc aacaaatttc tcgattattt tctccaatcc
     6781 tataattttt ttattcaagt agagaaaaat atactaagat t
//
LOCUS       DAJSVP010000110         6319 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSVP010000110 DAJSVP010000000
VERSION     DAJSVP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.114"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(99..1229)
                     /locus_tag="ON449_002439"
     CDS             complement(99..1229)
                     /locus_tag="ON449_002439"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3934963.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ON449_002440"
     CDS             complement(1878..3080)
                     /locus_tag="ON449_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3934964.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ON449_002441"
     CDS             complement(3140..3343)
                     /locus_tag="ON449_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3934965.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="ON449_002442"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="ON449_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="ON449_002443"
     CDS             4880..5692
                     /locus_tag="ON449_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3934966.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1877 a         1299 c          996 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaat
//
LOCUS       DAJSVP010000111         6250 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSVP010000111 DAJSVP010000000
VERSION     DAJSVP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.115"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(154..1605)
                     /locus_tag="ON449_002444"
     CDS             complement(154..1605)
                     /locus_tag="ON449_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3934967.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1926..2690)
                     /locus_tag="ON449_002445"
     CDS             complement(1926..2690)
                     /locus_tag="ON449_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR3934968.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2718..3416)
                     /locus_tag="ON449_002446"
     CDS             complement(2718..3416)
                     /locus_tag="ON449_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR3934969.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3428..4207)
                     /locus_tag="ON449_002447"
     CDS             complement(3428..4207)
                     /locus_tag="ON449_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR3934970.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4223..5167)
                     /locus_tag="ON449_002448"
     CDS             complement(4223..5167)
                     /locus_tag="ON449_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3934971.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5213..5992)
                     /locus_tag="ON449_002449"
     CDS             complement(5213..5992)
                     /locus_tag="ON449_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934972.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1725 a         1291 c         1020 g         2214 t
ORIGIN      
        1 atatttaatt cataaatata aatcttcatt ttaaatgtat gcccaaaaat ttatctgtaa
       61 tgcgtcatca aaatctagat tccaaatctt tatcaatttt tatccactca ttctaccact
      121 ttagtaatat tttctattta taacactttc gtcctacatt gatccccccc tctgaagtac
      181 agtttttact gtcttaaata aaattttaaa atctaacccc atactccaat tttttatata
      241 ttccgtgtca agttttgcta cttcttcaaa atcagtaata tcacttctac cacttacctg
      301 ccacattcca gtaattcctg gcttaatagc aattcttgca cgatgatgat aatcatattc
      361 ttcatactct gatttaaggc atggtcgaaa tcctataagg ctcatctttc ctgttagaac
      421 gttgaaaaat tgagggaatt catctaaaga cgtggatcta ataaattgac cgattcctgt
      481 cttttttgtt ccatctggaa gtattttatt tccgataatt cttggatcaa actccatctt
      541 aaacataaga ttattgctca tcttattttc tttcattaat tctacttttc tttcttctgc
      601 atccatatac atacttctaa acttatacat cttgaacggt ttaccattct gacctatacg
      661 ttcttgtgca aagaaaattg ggcctggtga attaatataa ataagtggag caataaagat
      721 aaaaataatt cccgttgcaa tacatccaac aatacctgca agaatatcaa ttgttcgttt
      781 tataaatgcc tgttgagttg tcataacatt gatacttgtt gtaagtactg tatattctcc
      841 tatacgacca atagattgtt ttcccaaagc actatctgat acttttgcaa gattcaaatg
      901 tacagtaagg cccatttcag agcatcgttc aataaattct ttcatacacg aagtactttt
      961 attcaaaata ataaagactt catctaccca tccctgacat aagtaatccg ctgcattttt
     1021 ggaatcagaa acaactgtca ctccctcaac atcttcacca atcatgcttt tatctatgat
     1081 aatgacacca tttattttat acattttata gttatgctct ttcacattac gaataactac
     1141 ttcagcaata ctactagatg taactataat aagtgatttg ttcccctcat ttttcatttt
     1201 atgtttcaag taatgtttcc ataagaggcg agttatatat gtaattacaa cgtataaacc
     1261 tcccatcaag aaaagaacga ctctagaata atcatttcca ccctgaacac taatgagata
     1321 caaacttcct aacagcacca acataatcgc ttgctttaca gaagaagcaa attctatata
     1381 gtaccctctt tttaatacac ctctatatgc ttcaaacatg aaaataacta aaagatctgc
     1441 cagttcaatg aagactgcca tgttttgata cattggaatc acataaggat tccaatctac
     1501 ctggcgtaag acatacgata gataaaaagc aagctggata cacaataaat ctaaaataat
     1561 aaaatcaata tgtttaaccc atccccttga acttttcttg tacattcctt tttactcccc
     1621 tctttttaat aaaataaatt gtattacttg ttttctctcc taaatatctt tctgaaggat
     1681 tattgactcg acaaattggc ttgtggatat tcttatatat agaacatccg taagccaact
     1741 taaaactcct taacaaaatt tttttactca tattgtagat caaaacaagt atccaagaaa
     1801 tcatatttta tttctttttt ctgcttactc aacaacagct aaaagaaaaa tcatcataaa
     1861 caaattcaac taagccaacg atccccttgt gaaatcaagt tttttcttga tttacgaata
     1921 aagagctaaa ataaaccaaa cttcttcttt tttacctctt catagactgg ataactgatt
     1981 tcatccccgt tgatcagatc ctttgcgact tgtttcatct gtactacttt ttccttacca
     2041 aagtcctttg caatctgctc atagcactct tttaagtaaa aggtgcgctt agagacccca
     2101 tgggcatcag aagcagccat ctgcacgaga ttatgtttga ccatcgtttt ggctgttttc
     2161 tggatgtctt tgccaaatac cccaacataa cttggcgcag tgagttgggc caaacatccc
     2221 atatctagga acggaagcaa atgatttggg tcttcgcgaa aatgcacgtt gcgttctgga
     2281 tggacgatta ctggtgtctt tcctagttct aacaactcaa aaaaaagccg ttctgcatat
     2341 aacggcactt ccagtgtcgg aaattcgatc agaagatatg tatcatctaa atctgtaaat
     2401 aatatttcat ctcggtggat gtcttcgatg agcgttccgg taatacgtac ttcttgtcct
     2461 tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg cttgtaacga agcgactaga
     2521 gaaatgactt gtgatttttc attgctgtat tttccattat tgtggtgtgg tgtgcataaa
     2581 atatgcgtga tgccttgctg aatcgctttt tcagccattg caatacttgc ttctaagttt
     2641 tctgccccgt catcgattcc tggtaggata tggcaatgta gatcgatcat ttctcttgtc
     2701 tcctctcttc ttattcctta atttccgtag taataaccag catctttgct atgttctgct
     2761 ccgttgtaca caacacctaa gactcgtgct tgtaccatct ccagcagttc ttttgcttta
     2821 gtcaaggatt ctttacgtga catattttca cgtacgacca ataaagtgcc atccgttttt
     2881 aaagacatga tctgtgcatc tgttacggct actactggtg gcatatcgaa tatcaccacg
     2941 tcaaataact gtcttgcttc ggataggatc tgatccattc ttggcgagct taacaattct
     3001 gatgggttgg gtggcttagg tccactaggc aaaatcgaca agttctcgac tggcgttcgt
     3061 tggatcacat ctgctacact tcctgatgaa ctaagtgctg tacttaatcc actggcattg
     3121 ttcagctgga atgtcttata gacgacgggt ttacgtaaat ctgcatcgac tagtagaact
     3181 cgttgacctg atttagcaaa aacgactgca atgtttgctg cagtggtgga tttcccttct
     3241 ccaggacctg atgaagtcac gacaatcgtt ttgatttgct gaccagctgc ggaggcaaac
     3301 tggatattcg tccggatcgt acgatattgt tcggcgattg gtgaagatgg gtttgttaat
     3361 gtgatcaaac tgactgcatg agtttgtgct gctttttgtt tctgtgttcg tgccattttt
     3421 cttcctccta gactcttgag cggcttctgc gtgatggggc tgttttatcg tttgtagctt
     3481 ttggtcgtgc aggagctgtt ccttttttac ggataccagc gttcaattct ttcgctgaca
     3541 tttctggaac tgttcctaag attgggaatc ctaatgtatc tgtcacatat ttttcatctt
     3601 tcactgtccg atcgagtaat tccaataaga atgccagacc tacacctacc atcattccta
     3661 acattaagcc agtcattaaa ttcaatttat tatttggcga aacaggtgtc gtattggcaa
     3721 ctgcacctga aatgatcgag attttatcaa cattcatcac atcttttgcg ttctcttgga
     3781 aaacttgtga agttgtgtta gcgatctgtt gagcagccac tgcattcgta cttattgcct
     3841 gaatcgagaa catttgtgaa ttttctgcct gattaactga gatggcatct ttgatttgtt
     3901 cagcagtcat tttcacattg tcttctgttt ctaaacgatc tttgacttca ttgagcacta
     3961 aatcactgac gatcatatct ttatacgtat tgatcatttg caggttcgta ttgacatcat
     4021 tgacatttgc agtgtctgat tgtggcagtg tcacgatcaa ttgtgcttgt gaactgtatt
     4081 ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc caagccagca aacattgaga
     4141 tgatgattat taccaagtgt ttgcgtaatg tttcaaaaat ttctttcaag ctgattgtct
     4201 cttccatttt ttctccactt tcctatttat tctctagatt catttgatct tttaattctt
     4261 ttttcacacg atcaagttct tgatcactga ctttttgata ggagatatta tcttgcatga
     4321 agccttctcc ttgcagctga tcttgcttaa tcgtaccaaa tgcctcccga taatctaaag
     4381 caattttttt catttcatca aagttcatgt ctgttttcat attcgactct agagcattca
     4441 aaacggattg atagttgctg acaccgttta gactcaatac ttttttcgct acgccttcta
     4501 cgatctttct ttggcgccct tgtctaccgt aatcaccatt tgggtcttca taacgcatac
     4561 gagaataaga taatgcctga tccccgttca atgtaatttt accgattgga aagtcatagc
     4621 catcttgtga gaaagtgagg tcattattga cttcgatacc accaactgca tcaacgagtt
     4681 cctttagtcc tttcatattg atagagacat agtgatcgat tgggatgtct acatattttt
     4741 gtacggtatc catcgccatg gcagctccgc cgaatgcgta agcgtggttg atcttgtctt
     4801 ttgtgtcacg acctacgatg tcgacatacg tatctcgagg aatgctgacg atcgttgtct
     4861 gtttgtcttc aggactgact gtggcaacca tcatcgtatc tgatcttcct tgatcgaccc
     4921 gacctaaatc tccagtatca atccccatca atagaacaga gaaagattct tgtttgtcca
     4981 gatcaactgg tttcgttcgt tctccttggg ttcgttccac tgattcgtaa gttttctcaa
     5041 tagaatgtga gagatccaga tataattttg ctcctactcc tacaattgct aatactaata
     5101 cgataagaac tgctaaaata atcaaaaata tttttttccc tcttgatttt cttcttcgtc
     5161 ttgccatagt ttcctccgtt tgctaacctt gcttgaatca acgttttaac tgtcaatagc
     5221 ttatattgct atagcccatt tgctgagcaa cagtttgtgc ttttgttgcg tatgcggctc
     5281 ttattttttc attccggatc tgctctattt gactgagcgc cgtcaaatat tgatcgtaag
     5341 tagcggtagc tgttacttga ccgtctttca gcatcgtatc gaatagctga tctagttgcg
     5401 tgacttgtgc aatttgggta tcagcgaatc ctagatattg cataacgact gttttccaag
     5461 cactatcctt aaaaaagctc atattttcac gtacatgggc gacatctgca cttgccaact
     5521 tttctttgat gatgacatca tccacagctt gctgccagtt tgatacgttt ttagtaaata
     5581 atttattgac agccgtctgg atcttcagtt ttgattctgc atcctctaac tgcgttaaga
     5641 ctgctttctt ttcttcttgg atcgcagcag cttctttagg taattctgat tccttgatgt
     5701 cataatcatc tgccgaaagt ttgatagaag atagtttatg tgacaaattt gaaactgttt
     5761 cttctgttat ttcaggattt aggaaaattt tttcttttgt actgtacagc ttatctactt
     5821 cttttgataa agaggtcacg gtgtcttttt gattggtgat ggcagattct gcgtaattga
     5881 tccgttgatg atattgccgc tgattattga gatggtatcc aaacaagcaa atcacaatag
     5941 cgcctgtgcc taccgccaca gtagccaata acttgacgaa cggcttttcc atagattcac
     6001 tcctaaatat tgatgataga taagtaaaga tgtgattcat tcacatcaga atgagcgctt
     6061 tttattttaa aagcataatt aaactaatat attcatagaa atatttataa acaggttttc
     6121 cctatataat cataccacat acagtttttt tgactaggga aattcctaat ttaaaagtaa
     6181 ataaaacagt aacaagatta taattaatcg ttttcatttt tatttcctca cttttcatga
     6241 gcgttttgaa
//
LOCUS       DAJSVP010000112         6250 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSVP010000112 DAJSVP010000000
VERSION     DAJSVP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.116"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            316..672
                     /locus_tag="ON449_002450"
     CDS             316..672
                     /locus_tag="ON449_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3934973.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            682..1581
                     /locus_tag="ON449_002451"
     CDS             682..1581
                     /locus_tag="ON449_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3934974.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2098..2829
                     /locus_tag="ON449_002452"
     CDS             2098..2829
                     /locus_tag="ON449_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3934975.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2974..5001
                     /locus_tag="ON449_002453"
     CDS             2974..5001
                     /locus_tag="ON449_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3934976.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5109..6206
                     /locus_tag="ON449_002454"
     CDS             5109..6206
                     /locus_tag="ON449_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3934977.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2166 a         1019 c         1284 g         1781 t
ORIGIN      
        1 ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc caatagttgt
       61 taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata aggttaaaca
      121 aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa
      181 gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt ccaataaatt
      241 gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat tattctatga
      301 catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt attgaagaat
      361 aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg tccatttgca
      421 gaaagccaag tcagttgcca attggatacg gcatttcgat tgatcattgt caaaaaagat
      481 acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg accaatcaaa
      541 atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt agaaccaact
      601 tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt
      661 cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct acatgtggac
      721 atcaataaat tcggcgaatc agagctgatg cagctttggc aagttttgca aaagcacaag
      781 atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg cctagctgtt
      841 gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa tcacgagttg
      901 gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt tttaaatgaa
      961 cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg gtacgactat
     1021 cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt gtattggtat
     1081 gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat gcttgcagct
     1141 gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt atccactcat
     1201 ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg ttggaacaaa
     1261 ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta ttcaaatatc
     1321 aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat cggtcacacg
     1381 atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg agattttgtc
     1441 ttgaaagaac agttgctaga cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc
     1501 catcacccag cttttcaggc ctatcaaaca caacatttgc aagaggaatt tgaacaagcg
     1561 atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat accttgtaaa
     1621 acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct ttttttgcgg
     1681 aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag tactagatgc
     1741 aagcgtgtat tctttttttg aacgataatc acaaaaaaaa atataatata cattttttat
     1801 ttaattataa ttaatattga ttgaagtcta ggcagtcaag tcacattttg atagatagga
     1861 gggggactta cgaattattt taaaggctat gacagctgta tttctagatt gtgtttgcat
     1921 caagttgcaa taaaaactag aaatcaaatc gggctgataa tcatttataa ctaatcgata
     1981 cataggacca aatataggtc tatgttcttt tatttcataa gtatcgtaaa acagaatttt
     2041 gctatggatt aatccaatcg actgatatgt ttagtaaata aagaaagagg aatgtcaatg
     2101 atgaaaaaag aaatcgtatt aacagcagca atgatgttat tcagcatggt agcaagtacg
     2161 acttttgttt ctgcaactga agtataccca aaggaatata acacagaagg aacgattaca
     2221 tttgaagcag gagatgaggg tgtaactcct cctgttgatc cagagaatcc tgatcccaat
     2281 aaacctgtcg atccatcaga tcctccaagc cctggaacag gaggggcatt atcgattgat
     2341 tatggatcaa aattcaaatt tgggactcaa aaaatttcta ctgcggataa aacgtattat
     2401 gcagctgctg atgtgatgaa cgatgggtct agaaaaccga catatgtcca agtgacagat
     2461 aggagaagta ccttatctgg gtggaaatta agtgtctctc aaccagaaca gtttaaaact
     2521 gccagtgggg atgaactagt tggagcacag ctgaaattta cgaaagggca ggctgtttct
     2581 ttggttgatc caacatatac gccgcaaacg gtgaattctg aattaacgtt gacaccggga
     2641 ggaaacaata cgttagcaat taatgctaaa tcaggaacag gtgtggggac ttgggtctat
     2701 cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt acccggtaaa
     2761 tctgtcaagt tagcgcaaca atattccact aaattagttt ggacattaga agatacacct
     2821 aacaactagt ttactgaaaa gaactgttaa aaataaatac gtagtcatat tgtcttgcga
     2881 ggtaatcgag caaaaattgt caaaggctag gctaaatttg tttggatacg aatagttcat
     2941 ctaggaattt aacgatcagg aggaaaagat atattgaaaa agaggaggat cgtgctcatt
     3001 ttaagcagtc tcttagtgac taatatcttt ccgatcatag ggtactcttt ggaattgaat
     3061 cagttggagc atggaaatag aagagacgaa gaaagcaaac tcacttcgac attcgacagt
     3121 ttaatagaat ccaaccagac acaagaaagc agtgaggatt taagtatttc gagtagtgga
     3181 gagaccactg acaacgtaga agctgagggt tcggacaaac aaggactaga agatgcacca
     3241 atttctgcgg ttgagctgcc tgacgatccc aatattattt ctatcgataa aatttttcaa
     3301 acacctatcg gtactagcac gtccatattg gaaaatggga agttactgca gttgaaccca
     3361 gcagaaaggt ctcaaagagg agctatttgg tcaaataaga agattagttt actctcggat
     3421 tttacattta aatcttattt atacttagga aatcaacgag atagagcggg agatggcatg
     3481 acctttacat tgactggcga tcctagaatg gctagtaatc ccagtcaagt tattggatcg
     3541 ccaggtatgg gaattggctc atattctact cgttctggcc aaccttacat tgcgaatgcg
     3601 ctctcgattg agtttgatac gtataaaaat agtggttctt ctgatcgaat ggatcgagag
     3661 ataactgctg ataatagagg gcatggacat gtcgcttttg tcacgcctaa accaaataat
     3721 aataattatt ctggagagca tactggcgtg acggtagctc ctacatttct ttctaatggc
     3781 acatggcgct tgttaataat taaatgggac gcggagcaaa gaagtcttac ttatgatttg
     3841 gaaggtgtcg gtcgctcttc tcggattata cctgatctca ttaggcagtt tggttctaca
     3901 gaagtaaatt gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct
     3961 gtcacgcgac ttccatcgaa tgtcacatca acagctgatg taaaagtcaa cgatagtgat
     4021 tatggggcca tcgctgaagc tggaggaaac gataagataa ctatcagaaa tacattgaag
     4081 gtcgaggggg attttcttga atataagaaa atgcagacta gtatcgacct cccacaagaa
     4141 ttaactattc ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg
     4201 ataaccggaa atcgagtcac gctaaatctg acagattatt ttattgctgg agatacgtta
     4261 ataatcgaat tggagacaac tttgcaggaa gtaattcctg aaaaagtact cactatgcat
     4321 tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga atgaaataaa cctaaagatc
     4381 gcaaagctga aggagataga tatccatgtt tattataaag ataaagacag tcagattgat
     4441 attgctgaaa tgaaaacaat cactgggaaa ataggagaga agtataaaga aactcctatt
     4501 gatattgaag ggtatgtttt tgactcggat tcagcgaata cagaaggcat cttctccgaa
     4561 aatacagagg atatctattt ttattacaga aaaggcaaac tttatctctc aactgctcct
     4621 caatatgtca attttggaaa acataaaata tccagtagac cattaagtga attcggacag
     4681 ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg gaagttggaa attacaattg
     4741 aaacaagaga aagttcttac aaatggagat tttgagcttc cagaagctct ttcctttgtt
     4801 acctctgaaa atacaacggt tatagggaca tccgctgtta cgattttcga aagtgatcaa
     4861 aagggtgaaa gcgatttgtc ctctctttta gattctacag ctcatcgtgg aatcagaata
     4921 aatattcctg tagaaaacca aagaatcgga acctttgagg ggaaactatc ttggatactt
     4981 gcagatgtcc caggaaatta acaggaagag aagaatataa caaaattatt gagtttcaag
     5041 aagggcacag gtaccatagg ctcttgtgtc ccacagagac aagattttgt tatagtcttc
     5101 tcttacatat gattaaagga gcgattttct tgagaccaaa acaacaatta tttataacac
     5161 tcctagttat tttaggggca ctttttaaca atatatcagt gtcagctagt gcatttaatt
     5221 tttcagtgac gccgataact tcagagaatc aaatcgacaa gagaaaaact tacttcgatc
     5281 ttcaacttga tccggatcag gaagtagaag taaaagcgga attgagaaat gatacagaaa
     5341 aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac caattcaaat gtgatggttg
     5401 aatacggaaa aaacgagatt gaaaaagaca tgagcctgat ctttgatctg gtagattatg
     5461 tgagctatcc acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg
     5521 tcaggatgcc gaatgagcga tttgatggtg tattggctgg tgggatcaca ttccaagaaa
     5581 tccagacgga aaaggaccaa acagaaacta aggatcaaag cttatcgatt gagaatgaat
     5641 acacctatat tattgctttg ttgatgcaac agacactgaa cgaagttgct ccgaacctta
     5701 ctcttcatga agttaaacca gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc
     5761 aaaatgatca aaaaacctat atcaatcaag tagttattga aacaaaaatt acaaaaaaag
     5821 gacattctga agttttatat caagaggaaa aagaaggatt gcagattgcg ccgaatacaa
     5881 atttttcttt tccaactgct ttaaatggtc aaccgctaac cccaggtgaa tatcacctta
     5941 caatgactgt tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata
     6001 ctacgatcaa ctataccaat caatgggtat ttgaaaaaga ttttacaata gacggaaaag
     6061 tagccaagga attgaacacg acggatgtta cgattaaaaa agacaataat tggatttata
     6121 tcttagtagc ttcacttttc ttattgttta ttttattatg catacttttg tttgttttga
     6181 gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa atagtaaatt tttattattg
     6241 taggatggtt
//
LOCUS       DAJSVP010000113         6102 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSVP010000113 DAJSVP010000000
VERSION     DAJSVP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.117"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            143..790
                     /locus_tag="ON449_002455"
     CDS             143..790
                     /locus_tag="ON449_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3934978.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    919..1135
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1183..3828
                     /locus_tag="ON449_002456"
     CDS             1183..3828
                     /locus_tag="ON449_002456"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3934979.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4138..5451
                     /locus_tag="ON449_002457"
     CDS             4138..5451
                     /locus_tag="ON449_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3934980.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5441..6094
                     /locus_tag="ON449_002458"
     CDS             5441..6094
                     /locus_tag="ON449_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3934981.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
BASE COUNT         2018 a         1038 c         1330 g         1716 t
ORIGIN      
        1 ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt ctcacaccct
       61 tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta aatatgtgaa
      121 attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa aagcaactgc
      181 gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag gtataagtag
      241 aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga tccgcagaga
      301 cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc cttttcttat
      361 cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat tagtaggata
      421 tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa atctaaatat
      481 cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg gagaaatgat
      541 ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag tagcaatctc
      601 tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag gaaatgtaac
      661 cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca atgttcaata
      721 cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt actcagaagt
      781 tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc gtaattcttt
      841 caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg actaaaaaaa
      901 aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca cattcattct
      961 tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt agcttgggag
     1021 tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac ttgagaggca
     1081 ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc tagaatacga
     1141 aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga agaaaataac
     1201 ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca ttggttagaa
     1261 caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat cgtaatcccg
     1321 cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac cttgcaagat
     1381 atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc tggtatggac
     1441 catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca agggatttct
     1501 cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa agaagagtat
     1561 gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta cagtagggaa
     1621 cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt taccttatac
     1681 gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca agcacgaaca
     1741 gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta tcatatgagc
     1801 tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc agaaacgatg
     1861 ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc gctcatcggc
     1921 aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga tgaatatgta
     1981 gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc aaacgacttt
     2041 gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga tggtacgatg
     2101 aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg
     2161 caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca cagtgtgggt
     2221 cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa
     2281 atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc agtggaattc
     2341 ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca tgattgggta
     2401 atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa agaaacaggt
     2461 gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca agatcctgat
     2521 gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg ctggccagat
     2581 acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac cggttatgac
     2641 atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac tggcgaacgt
     2701 ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg gaagatgagt
     2761 aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg agcagatgct
     2821 ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt cagctatgaa
     2881 aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg tttcgttatc
     2941 atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa atcagtggct
     3001 gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga gctatttgat
     3061 cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga tgatttttgt
     3121 gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt ttcaaaacaa
     3181 accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt gcatccaatc
     3241 atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa ttctctagta
     3301 gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc taaagggatg
     3361 gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt gaatacacca
     3421 ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga tcaattttta
     3481 gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac gatatcaagt
     3541 gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga aatcttcttg
     3601 ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa agaactggaa
     3661 aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct
     3721 aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta tttagagaag
     3781 caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag
     3841 agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg ttacaggaaa
     3901 gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa ttttgtcgca
     3961 tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc atgtatcaag
     4021 agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct ttgtcatact
     4081 tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga attgttgatg
     4141 acgattgaag aagcaataga atggatccat agtcgattac catttggttc acgtcctggc
     4201 cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa agtaaaaacg
     4261 atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag aagcctatta
     4321 gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac gttcaatgaa
     4381 cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct tgtaaaaaaa
     4441 tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat tactgaattc
     4501 gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga cgtagcgatt
     4561 atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc aatgctgacg
     4621 ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat tgaagaaatc
     4681 gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg aaatattgcc
     4741 ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta
     4801 tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg agaagtattt
     4861 gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt agggcaacat
     4921 caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca attggaaaag
     4981 ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg gccaggaaga
     5041 atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa tacgcatgca
     5101 atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat caacattttg
     5161 ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca gcaagtacca
     5221 aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt aagtgaagta
     5281 tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg attagcagat
     5341 atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact ttatttcatt
     5401 tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata atgcagtgat
     5461 ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag ccacacaagt
     5521 agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat tcttaggtgt
     5581 ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg gcaaaacaag
     5641 tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt cgcaaggagc
     5701 ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac atcagattcc
     5761 caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga agaagaacaa
     5821 tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag caaaaccaga
     5881 cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag aaacattgat
     5941 tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc cggtagtcat
     6001 ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg gggttgtttc
     6061 ttctttacat gaaatccctg cattgttaaa ataaaaatcc at
//
LOCUS       DAJSVP010000114         6101 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSVP010000114 DAJSVP010000000
VERSION     DAJSVP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.118"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(312..797)
                     /locus_tag="ON449_002459"
     CDS             complement(312..797)
                     /locus_tag="ON449_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934982.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="ON449_002460"
     CDS             complement(936..1151)
                     /locus_tag="ON449_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR3934983.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1375..2175
                     /locus_tag="ON449_002461"
     CDS             1375..2175
                     /locus_tag="ON449_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3934984.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2194..4062
                     /locus_tag="ON449_002462"
     CDS             2194..4062
                     /locus_tag="ON449_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3934985.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4055..4546
                     /locus_tag="ON449_002463"
     CDS             4055..4546
                     /locus_tag="ON449_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3934986.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4534..5088
                     /gene="srlA"
                     /locus_tag="ON449_002464"
     CDS             4534..5088
                     /gene="srlA"
                     /locus_tag="ON449_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3934987.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5106..6101
                     /locus_tag="ON449_002465"
     CDS             5106..6101
                     /locus_tag="ON449_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3934988.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2157 a          968 c         1222 g         1754 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     1201 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     1261 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     1321 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     1381 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     1441 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     1501 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     1561 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     1621 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     1681 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     1741 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     1801 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     1861 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     1921 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     1981 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     2041 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     2101 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     2161 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     2221 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     2281 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     2341 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     2401 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     2461 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     2521 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     2581 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     2641 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     2701 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     2761 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     2821 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     2881 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     2941 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     3001 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     3061 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     3121 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     3181 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     3241 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     3301 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     3361 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     3421 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     3481 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     3541 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     3601 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     3661 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     3721 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     3781 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     3841 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     3901 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     3961 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     4021 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     4081 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     4141 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     4201 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     4261 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     4321 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     4381 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     4441 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     4501 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     4561 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     4621 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     4681 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     4741 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     4801 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     4861 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     4921 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     4981 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     5041 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     5101 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     5161 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     5221 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     5281 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     5341 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     5401 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     5461 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     5521 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     5581 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     5641 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     5701 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     5761 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     5821 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     5881 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     5941 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     6001 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     6061 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAJSVP010000115         5954 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSVP010000115 DAJSVP010000000
VERSION     DAJSVP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.119"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            721..1455
                     /locus_tag="ON449_002466"
     CDS             721..1455
                     /locus_tag="ON449_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3934989.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1471..2640
                     /locus_tag="ON449_002467"
     CDS             1471..2640
                     /locus_tag="ON449_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3934990.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2656..3651
                     /gene="lacD"
                     /locus_tag="ON449_002468"
     CDS             2656..3651
                     /gene="lacD"
                     /locus_tag="ON449_002468"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3934991.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3730..4656
                     /locus_tag="ON449_002469"
     CDS             3730..4656
                     /locus_tag="ON449_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR3934992.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4675..5925
                     /locus_tag="ON449_002470"
     CDS             4675..5925
                     /locus_tag="ON449_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3934993.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1998 a          970 c         1217 g         1769 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaaa gtttgaaaaa tgctatttcg aggtagaagg atattatata
      121 agcacggttg actaaacgaa gtgataataa aaaaagttat tcaagaataa gaaaaattat
      181 gatacgaatt cagataaact ggagaataca gagaccttta aaagggataa gaaacaacat
      241 aaattcctct taagagagaa cgaaaggaca aagccagcaa cttgatacac gagctagtat
      301 tagaggaatt tattctcata gactcccttt tgaatacgtt tcaactttaa ttttcatgtt
      361 cataaatgat ttgaaaccaa agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg
      421 ttcaattcac tggtaagtag tcatttgata gtttgaggca aattgttgat tgatgaaggg
      481 taggagctaa aaaaagatac cagttgaaga gagaaaaaga aaaaaataga gggtgttctt
      541 gaatgaaaaa agttggaaaa ttatttttca tttcctttta ttcctattaa agtcagtgat
      601 gtcaatcata ttaacaagac taaaaatgaa aataaaaaaa agattgttct tagtattgac
      661 tcataaaaaa taccactact atacttacat tgtggttata gccagttagg agagacatcg
      721 atgcaaaata cctttcaatc aaaatcactt tattatcagc tggtagaaat attgaaacaa
      781 cgaatcgaaa acagtatggt cgctcatgac aagctgccat cagaaagaga gttgaccagt
      841 ttttatggcg ttagtcgcac gacggtgcga ctagcattgc aagaactgga aaaaatgggc
      901 tacatttatc gacaacatgg aaaaggaacc tttgtttcgg aattaaggac aaatgcgact
      961 gatctagctg gcgcatatag ttttaccgaa caaatgcggg caatgggcaa acatcctgaa
     1021 accaagatat tggccttcaa gaagattgaa gcaactaaat atttagcgag tcatctgaat
     1081 gttcaactag gtgagccttt atttcaatta gaccgattgc gcttagctga tgatttgcca
     1141 atgatgatcg agaaatccta tttgccagtg aagctattta tgtccctttc tcaagaaatt
     1201 ctatttctga aacctttata taatttgttt tcagagaatt tcaatcaaat cgtacggttg
     1261 gctgacgagg aattttatgc cagcattgtc cagaaagaag atagtgagtt gctaaatatt
     1321 ccagaaggat caccagtgct ccatttagtt cgctcaacct ataatctcga gaacgaagtg
     1381 attgaattta cattaagtgt agcgcgggct gataaatttc attataagat caggcatctg
     1441 cgttccacta actgaattgg aggaaagaaa atgtttatgt tctcaaaaga agaactacga
     1501 aagctaggag cagaaaatac tacccgagaa attcgacagc aaccggaact gtggcatgag
     1561 acgtttgacc gttatcaaga aacagttgca gatttagatc gtttgtttac ggatgcgaaa
     1621 caacatgctg gagagcgacc aatccgagtc atttttacag gggccggaac ctcgcaatat
     1681 gtgggagata cactaattcc gtttcttact agtcaaggaa atacgaacca gtttattttt
     1741 caaagtattg gttcgaccga tatcgttgct acgccaaaag aatacctgat tcctgatgag
     1801 acaacgatct tggtctcatt tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt
     1861 aagatggcca atcagctggt caacaagatc tatcatatcg tgatcacctg tgcaccagac
     1921 ggattattgg ctacacaaat gcctacaaaa aaagaaaatc tagttttact aatgccaaac
     1981 aaagccaatg acgctggttt tgctatgaca agcagtttca gctgtatgac gctgactgcg
     2041 ctacttattt ttgatcaagc aagcaatcag caaaaagaaa gatatgttca tcaattaagc
     2101 cgactaggag aagaagtcat ccaacgagaa tcagagatcc aagggtatct ggcatcagac
     2161 tttcatcgca tcatttactt aggttcggga agcttaggcg gattgactag agaagcgcaa
     2221 ttaaaagttc tcgagttaac tgctggaaaa atcgcaaccg tatttgatac atctttagga
     2281 ttcagacatg gtcccaaatc ttttgtcaat gagcaaacat tggttttcct gtttgtcagc
     2341 aatgactcat atacgcagaa atacgatgta gacatgctgg aagaaatcaa aaatgacggt
     2401 attgcccaac gcgttgttgg aattggtcaa aagggttcat tgtttagtgg tgattccttt
     2461 gaattagttg cagattcgtt actgccggat ggctatcttg ctttagcaga tatcatggta
     2521 gcccaaacga tcgcattact ttgttcggtg aaggttggta atacaccaga tacaccatcg
     2581 ccaagtggaa cagtcaatcg tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag
     2641 gaaagagtgt gaagcatgtt agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta
     2701 tcgacgccag aaggcattat taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa
     2761 atgatgtcgg ctttagggat ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta
     2821 atttcccaag aattgactcc ttatgcctca gcaattttat tggatcctga atacggactg
     2881 cctgctgcac agaagcgtgc agcagcgagc ggtctgctgt tggcatatga aaaaactggt
     2941 tatgatcaaa ccgtacccgg ccgcttgccg gatctgctgt cggactgttc agttctgcga
     3001 ttgaaagagt taggtgcgga tgcggtgaaa tttttactgt attacgatta tgaagaagca
     3061 gaggaaatca atcggcaaaa gcaagttttt gttgaacgaa ttggaagcga atgcatggct
     3121 gaaggactgc cattttattt agagttattg tcatatgatg ctaaaaatgt agatacgaaa
     3181 tcgctttcct atgccaaagc aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg
     3241 aaaacccagt ataaagtaga tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt
     3301 gaaggaaatg caacagggga gatcgcctat acacgtcaag aagctgccgc ttattttaaa
     3361 gaacaaagcg atgcaacaga tttgccgtat atcttcttaa gtgctggggt atcagcggca
     3421 ttattcatgg agacattgcg ttttgccaaa catgctgggg cacgattcaa cggtgtactc
     3481 tgtggcagag caacttggaa agatgcagtc gctcctttcg ttgaaaaagg agaagcggca
     3541 actttagaat ggctgacaga acaagggaca caaaacattc gtcaattgaa tgaagtgata
     3601 agagaaactg ccatcccatg gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa
     3661 tatattagta gtgataagag atatgtgtga ttataattta tcattattga aactgggagg
     3721 atattttata tgaataaaag attgttagat tattcagctt cagatttttt gaaagctaag
     3781 ccgatggaat taaagcaggc aattttggct tctgaaggta gaacaattat ggcagaaaat
     3841 gtgccaagta gctgtcctta catgggaaaa tcagtgacaa atgcagaatt ggagagagct
     3901 gctggagctg atttaatttt gtttaatgca ctagatcttt ttaatcctca aatttcaggt
     3961 gtacctttaa ctctaaaaga gaatccaata atctggatta aaaaagcttt aggaagagct
     4021 attggtgtaa atttagaacc tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa
     4081 atttcaagtg gtagaatcgt ttctcctgag actttaaaaa aggcaaatga attaggcttt
     4141 aattttattt gtttaactgg aaatccagga acaggagtat ctaatagaac tattgagaaa
     4201 gcaataacat tatccaaatc aatttttaat ggattaatta tcgctggaaa aatgcatggt
     4261 gctggtgttg atgaaccaat tatgaactta gatatagcaa aaagatttat taaaacaggt
     4321 gtagatattt tattagttcc tgctccatac actgttccgt atttcatgga aagtgatttg
     4381 cgaggaatcg ttgattatat aaaagatttt aatagaaata aatctattga ggaaaaagtt
     4441 ctagtactaa cggctaatgg aacaagccaa gactcttctg accaagaaac tattaaaaaa
     4501 attgcattag catcaaaagc atgtggagct gatattcaac acatagggga ttctttaaat
     4561 ggtatttgtc ttccagaaaa tatttttgct ttgggtcaag caattcgggg atatcgtcat
     4621 caaattatta tgttaggaaa atctaattat cgttaattaa ggaggaaatg aaagatgatc
     4681 gacaaagttc aaaaaatagc tgcaaaaatt agtaatcaaa gacatctact agctttaaga
     4741 gacagcatgg ctgcagttat gccattaatc attattggtt ccttttttat gttattagca
     4801 aatttcccga tagaaggatt tacaaaatgg ttatctgaaa ttggagtaca atcctttttg
     4861 aacaaagcat cagattcaac atttggtatt attggattag ctgttaccta ctctttggga
     4921 cacaatctag caaagactta tggtgtagac tcattatcag caggattatt atctttgagt
     4981 ggatatgttt tacttacacc tttattggat gcagatggtt ctataggatt tccaacaaaa
     5041 tactttggta cctcgggtct ttttgttggt attattgtag cacttgtatc tacagaaatt
     5101 ttccggtggt ttgtacttaa aaatattaca attaaaatgc cggcaagcgt tccaccaaat
     5161 gtaagccgag ctttcgttgc tattattcct ggcttttttg tcgttctact atggtttatt
     5221 gttgttgtaa tttgttataa acttggaatt gaaaatgttc atgctcttat tgcagataca
     5281 ctcgcaaaac cactatcttt actgacgaaa actttaccag gaattatatt ggttatactt
     5341 attcaatgtt tcttttggat gtttggtatt catggtgcac aggtaacggg gccaataatt
     5401 gaaccgttat tattgcaaaa ctctgatgta aatagaattg cttatcaagc tggaaaagaa
     5461 cttcctaaca ttataactta tgaattctta tataactttg ttttttctgg tggtgcaggt
     5521 tgtttatttg cacttgccat tttaacattt ttcttttcaa agagtcagga aaataaaact
     5581 ttaggtaagt tatcaatcgc tccagtaagt tttcaagtag cagagcccct tctatttggg
     5641 tttccaacta tattaaatat aaaaatgtta attccatttg taactgcacc agtagtaaca
     5701 acattaatta cttatttttc aatgagtatg ggattagttg caaaacctgt aggagcaaca
     5761 atcccatgga caactccacc gataattgcc ggatttttag cttctggtgg acgaataagt
     5821 ggtgcagtga ttcaagtaat aacaattgca ataaatgttt tgatatacta tccattcttt
     5881 aaattagatg ataatgccaa gctaaaaagc gaaaaaaatg attagaacta cctatatgtt
     5941 tctaactgta gtta
//
LOCUS       DAJSVP010000116         5932 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSVP010000116 DAJSVP010000000
VERSION     DAJSVP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.120"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(482..985)
                     /locus_tag="ON449_002471"
     CDS             complement(482..985)
                     /locus_tag="ON449_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3934994.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1344..2369
                     /locus_tag="ON449_002472"
     CDS             1344..2369
                     /locus_tag="ON449_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3934995.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2692..3570
                     /locus_tag="ON449_002473"
     CDS             2692..3570
                     /locus_tag="ON449_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3934996.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4046..4162
                     /locus_tag="ON449_002474"
     CDS             4046..4162
                     /locus_tag="ON449_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3934997.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5090..5428
                     /locus_tag="ON449_002475"
     CDS             5090..5428
                     /locus_tag="ON449_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934998.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5522..5752
                     /locus_tag="ON449_002476"
     CDS             5522..5752
                     /locus_tag="ON449_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3934999.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2015 a          891 c         1088 g         1938 t
ORIGIN      
        1 aaataacgac acagaaaata ccgtgtctca gatttctcta ctacttctaa aaactactgt
       61 ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca gaatatactg gcagaatcaa
      121 ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat tacttataaa
      181 acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga cggaattcct
      241 ttctagattg agatattgac taggaagaat gttaagattt taagccttta agctaaaaaa
      301 aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct
      361 gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa tgactgaata
      421 aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt tacgaacgat
      481 tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga aataagaata
      541 aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga tgtttgttta
      601 gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc aaatcagtga
      661 tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag cttacaagga
      721 aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc
      781 caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc gtattttcaa
      841 aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg agaaattgtg
      901 gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa taaatcccta
      961 ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta taccatcgaa
     1021 aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa
     1081 aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag atagcgagtt
     1141 gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg ttttttgttc
     1201 taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa gaaatagttg
     1261 ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag ctttttttcc
     1321 aattactagg aaggagggat agattgttca agacttattt tcgtttcaat aaaatacaag
     1381 ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta tggttaggtt
     1441 acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt acttttttct
     1501 attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc ggttgcggac
     1561 tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt gaaccatttg
     1621 ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat atttctaaac
     1681 caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag ctgccaatcg
     1741 ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg
     1801 cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa gcaactttaa
     1861 aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac agcgctgcct
     1921 tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat tggttgacct
     1981 tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat cgacaagtcc
     2041 aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt
     2101 ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca caatttctag
     2161 ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg atgctatcta
     2221 cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta gggatcgtca
     2281 taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg caacattatt
     2341 taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa aagaggttat
     2401 gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt ctcagcatta
     2461 agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata tttattctat
     2521 caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc aatgctttcc
     2581 ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag ttagtgtaca
     2641 ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac aatgaggttc
     2701 aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca aattctggca
     2761 gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc aaaaaacatc
     2821 cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa agcaaaggcg
     2881 actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt agctatttat
     2941 ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat tccaagaact
     3001 atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt ccaagtaaca
     3061 ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg cttgatcttt
     3121 cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac ggatctaaga
     3181 ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt atctcagaat
     3241 aagataagag atagttacga aagtgccatt cgtttttttg aactttattt tcctgattac
     3301 cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac taaatggttg
     3361 aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc agttgatgaa
     3421 caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat acatgattat
     3481 ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg cgagcacatt
     3541 ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt ttaaaagagc
     3601 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     3661 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     3721 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttagtgttct
     3781 atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat taaagttaac
     3841 ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat ttccagagat
     3901 aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat tttaactttt
     3961 gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc atgactacaa
     4021 tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg cataccgccg
     4081 cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca aagatgtgaa
     4141 acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag ccgaaaaacg
     4201 tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa ctgctgttga
     4261 ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt gataccattc
     4321 cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt ataaaagtat
     4381 tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta gtattgatca
     4441 ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa tggaaacgta
     4501 actacataat caaaggtttt gcgaaatatt tcatgaaata tttcatgtag aagcgagcaa
     4561 tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc aatatctcta
     4621 acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat ctctttctat
     4681 ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa aataaaaaag
     4741 cagttactat tgctagaata acatcaagga ataataagtt ttggttttca gataatagtc
     4801 gataagttgt gtattggaat tttagataca gtaataaagt atttttcatg aaattttgtg
     4861 agaatcacta ttacaagtat ctttaataaa attaatggtt aaggaggaac ttaaagtaga
     4921 atgtaaatga atagttgtag tcagtcgatt gtgatactag actcaaagtt gcaggaataa
     4981 gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga agggcttttt tttatacttt
     5041 gttgattgta gtgtttattt tccctctgtt tagccaagta acattttcta tgactcaacc
     5101 agtttcaagt gaggaacatg ataacctttt tcgtgctaca gaagagttta agaaatatca
     5161 ggaaaatatt gttccggaga ctccattagc tagtgagcca attactgata ttgatggaaa
     5221 ccaaatagga tggtatatac agtacgaagt tatggtggta aataatgact caatagtaaa
     5281 aaatacagta ggcttagagc taacaccgat tgaaaaggtg agttcattgt taacgtatat
     5341 ttatactgat gaaagtgacg agactgagat cattttaacg gattactcaa aaattgctac
     5401 tgataaaaag tatatattgt ggatgtagac actagtgaaa tactttatga tatagaaatt
     5461 gatgaaaatt tactagatgt agcacaagaa atcgatcatc aaaagcagga agctattcga
     5521 tatgctggta gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa aagttagaga
     5581 agatgaaaat gagtggaaag aattcaagag tcgctactca attaactcga ctccgacatt
     5641 tacagtttac agagaaggat cgatagaaaa aactgttttt tggactaaag aaagtggaat
     5701 gtctttagct gaagtagaag agtttttgga ttatgttagt atgcaacagt aagggagttc
     5761 ttattatgaa aaattttgac acatattatg ttattgtttt ttcgatatct ttgctgttaa
     5821 tattactaac tttaatgcag tataaagagt atccagagta ttttatttta acttttgtat
     5881 atttagtaag tagctacaca cttctatata aattcagaag ttcttctaga gt
//
LOCUS       DAJSVP010000117         5914 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSVP010000117 DAJSVP010000000
VERSION     DAJSVP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.121"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1403)
                     /locus_tag="ON449_002477"
     CDS             complement(<1..1403)
                     /locus_tag="ON449_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3935000.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFN"
     gene            complement(1414..2082)
                     /locus_tag="ON449_002478"
     CDS             complement(1414..2082)
                     /locus_tag="ON449_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3935001.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2212..2796)
                     /locus_tag="ON449_002479"
     CDS             complement(2212..2796)
                     /locus_tag="ON449_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3935002.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3127..3360)
                     /locus_tag="ON449_002480"
     CDS             complement(3127..3360)
                     /locus_tag="ON449_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3935003.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3375..4325)
                     /locus_tag="ON449_002481"
     CDS             complement(3375..4325)
                     /locus_tag="ON449_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3935004.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4322..5164)
                     /locus_tag="ON449_002482"
     CDS             complement(4322..5164)
                     /locus_tag="ON449_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3935005.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5166..5885)
                     /locus_tag="ON449_002483"
     CDS             complement(5166..5885)
                     /locus_tag="ON449_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3935006.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1989 a         1068 c          719 g         2138 t
ORIGIN      
        1 tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat atcttttttt
       61 attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa ttccggatca
      121 tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc agcaatcctt
      181 tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa attagttttt
      241 gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa atcaaacggt
      301 aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg ctgatcttta
      361 ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa aaattggtaa
      421 tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc gattatcttt
      481 cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt ccccacaccg
      541 tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat gtcttttaga
      601 atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt ttcgatatag
      661 ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt
      721 tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca
      781 attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt
      841 tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac
      901 ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat agaaattttc
      961 tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat
     1021 aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc
     1081 atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga
     1141 cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt
     1201 tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt
     1261 aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct
     1321 ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa
     1381 gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat
     1441 agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact
     1501 tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat
     1561 catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa
     1621 aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg
     1681 ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg
     1741 tattttttct aaagaggata ataattcatt gctagtatag acactaacca acaaccatga
     1801 gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt
     1861 aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat
     1921 caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat
     1981 aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa
     2041 aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa
     2101 taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga
     2161 tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag
     2221 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
     2281 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
     2341 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
     2401 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
     2461 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
     2521 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
     2581 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
     2641 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
     2701 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
     2761 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
     2821 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
     2881 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
     2941 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
     3001 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
     3061 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
     3121 agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa
     3181 ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga
     3241 tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt
     3301 tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat
     3361 acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc
     3421 atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt
     3481 gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc atccacactg
     3541 ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt
     3601 tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta
     3661 tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc
     3721 ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac
     3781 ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt
     3841 tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc
     3901 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
     3961 tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct
     4021 ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca
     4081 tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa
     4141 tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact
     4201 acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc
     4261 ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc
     4321 atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa
     4381 ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat
     4441 aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca
     4501 tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa
     4561 caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca
     4621 tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa
     4681 aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa
     4741 cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat
     4801 gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag
     4861 aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt tttataaaag
     4921 taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta
     4981 aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag
     5041 acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag
     5101 atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa
     5161 gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt taaattttct
     5221 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
     5281 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
     5341 tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct
     5401 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
     5461 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
     5521 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
     5581 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
     5641 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
     5701 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
     5761 gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
     5821 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
     5881 aacataactt tctccttcta aatgataatg atta
//
LOCUS       DAJSVP010000118         5784 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSVP010000118 DAJSVP010000000
VERSION     DAJSVP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.123"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(247..954)
                     /locus_tag="ON449_002484"
     CDS             complement(247..954)
                     /locus_tag="ON449_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935007.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1019..1855)
                     /locus_tag="ON449_002485"
     CDS             complement(1019..1855)
                     /locus_tag="ON449_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3935008.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1873..3282)
                     /locus_tag="ON449_002486"
     CDS             complement(1873..3282)
                     /locus_tag="ON449_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3935009.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3296..5119)
                     /locus_tag="ON449_002487"
     CDS             complement(3296..5119)
                     /locus_tag="ON449_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3935010.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1772 a         1258 c          945 g         1809 t
ORIGIN      
        1 tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc
       61 caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac
      121 ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat
      181 aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata
      241 atctttttac ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac
      301 tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga
      361 aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg
      421 tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt
      481 atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt
      541 aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg
      601 tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc
      661 agttctttca atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac
      721 atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct
      781 tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct tattattaat
      841 ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac
      901 ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc
      961 aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc
     1021 agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac
     1081 ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac
     1141 gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca
     1201 ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat
     1261 caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta
     1321 ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg
     1381 caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga
     1441 tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc
     1501 gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca
     1561 aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa
     1621 ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct
     1681 tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa
     1741 ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc
     1801 cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat
     1861 tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct
     1921 ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa
     1981 accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa
     2041 ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc
     2101 tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc
     2161 cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa
     2221 tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg
     2281 attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct
     2341 agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt
     2401 aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt
     2461 aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac
     2521 tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc
     2581 ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc
     2641 tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg
     2701 attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc
     2761 tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc
     2821 accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt
     2881 taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat
     2941 cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt
     3001 ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa
     3061 agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc
     3121 cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg
     3181 ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca
     3241 taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac
     3301 taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt
     3361 aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg
     3421 cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa
     3481 atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt
     3541 caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt
     3601 ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca
     3661 atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta
     3721 ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat
     3781 cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa
     3841 taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc
     3901 caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg
     3961 caccaccaat catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg
     4021 aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact
     4081 ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag
     4141 ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga
     4201 ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca
     4261 aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt gctataagat
     4321 cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa
     4381 ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg
     4441 gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat
     4501 ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg
     4561 aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa
     4621 actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa
     4681 tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga
     4741 cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg
     4801 tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc
     4861 cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat
     4921 tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt
     4981 tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg
     5041 cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc
     5101 ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac
     5161 cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac
     5221 atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac
     5281 agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca
     5341 catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct aatattaact
     5401 ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca
     5461 aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa
     5521 tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac
     5581 ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag
     5641 gtatcaatag aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg
     5701 atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca ataaaaatac
     5761 caaacatatc cataataatt tttt
//
LOCUS       DAJSVP010000119         5471 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSVP010000119 DAJSVP010000000
VERSION     DAJSVP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.124"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(52..522)
                     /locus_tag="ON449_002488"
     CDS             complement(52..522)
                     /locus_tag="ON449_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3935011.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ON449_002489"
     CDS             complement(681..1139)
                     /locus_tag="ON449_002489"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3935012.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ON449_002490"
     CDS             complement(1310..2455)
                     /locus_tag="ON449_002490"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3935013.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ON449_002491"
     CDS             complement(2468..3385)
                     /locus_tag="ON449_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3935014.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ON449_002492"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ON449_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3935015.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4516..>5471)
                     /locus_tag="ON449_002493"
     CDS             complement(4516..>5471)
                     /locus_tag="ON449_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3935016.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1653 a         1014 c         1037 g         1767 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc ctgtgtaaaa tactatacaa
     4501 tgctctcact attttttact tggcaaattt aatatatttt cttgatgaag aaagccactc
     4561 atcgtgttga tcaataagga ctgctccgat taaacgattg gctgaaacgc agtttggaaa
     4621 gatacgaata attttttctc ttctgcgcac ttcttgattc agtcgttcaa ttagattggt
     4681 actctttagt cgattgtggg aatttccttg tacggtatat tgaaaggcgt cttcgaatcc
     4741 atcatccaat gatgcgcaag cttttgaata ttttggttga tcgatataat catgaatcaa
     4801 tcgattttta gcctcacgag ctaagttaat atctgtgaac ttaaaaattc ctttaacagc
     4861 ttctctgaaa gattttgaat tttttttagg aatggtggta aagatatttc ttaggaagtg
     4921 aacttggcat ctttgccaac ttgcgttggt gaaggatttt ctaatggcag agactaatcc
     4981 tttgtgcgca tcagaaataa cgagttctgt accttgtaaa ccgcgttctt ttaggtattc
     5041 aaaaaatgtt atccaggtct cttcgctttc gccactttga atcatgaagc cgataatttc
     5101 acggtcgcca tctttggtta ttccaatcgc tatatgacag ctttttgaga gtactcgatt
     5161 ttcttctcgt acttttatat agagtacatc ggtcattaag taaggataat ttttttctga
     5221 taataaacga ttctgccact cgttaaccat tggttctagc tgttctgtta agctagaaac
     5281 gaaggactta gagacggatt taccacaaag ttcttccaca atttttgata ctttacgagt
     5341 tgaaacgcct gatacataca tttccaacat tgaagcaatg agggcttttt cgtttcgttg
     5401 ataacgttca aacactgtgg gtgaaaaatg gccatcacgt gttctgggta cttttaattc
     5461 tagcgtgcct a
//
LOCUS       DAJSVP010000120         5429 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSVP010000120 DAJSVP010000000
VERSION     DAJSVP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.125"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(151..426)
                     /locus_tag="ON449_002494"
     CDS             complement(151..426)
                     /locus_tag="ON449_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR3935017.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(398..1351)
                     /locus_tag="ON449_002495"
     CDS             complement(398..1351)
                     /locus_tag="ON449_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3935018.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1963..3456
                     /locus_tag="ON449_002496"
     CDS             1963..3456
                     /locus_tag="ON449_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3935019.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3591..3887
                     /locus_tag="ON449_002497"
     CDS             3591..3887
                     /locus_tag="ON449_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3935020.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3988..4839
                     /locus_tag="ON449_002498"
     CDS             3988..4839
                     /locus_tag="ON449_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935021.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4860..5143
                     /locus_tag="ON449_002499"
                     /pseudo
     CDS             4860..5143
                     /locus_tag="ON449_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1869 a          836 c         1014 g         1710 t
ORIGIN      
        1 tttagtatag taaaaactag aaacaataaa atttgtgaat gttgtatacc cccaaacaat
       61 acacatattt tgaaggatta ggattaataa agctaaaaat atagtaagta tactaaatat
      121 atttactata tctagtatac ttactatatt ttaatctttt gatcgtctat tatatataat
      181 ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt gcaagtttga ctatttcata
      241 cataggaata tcttttgcat aactaagatc ccgaatggtt tttagtacgg gaggatctac
      301 ttgaactggt tttctttctt tcgcagtgta ctttcgttcc cctaactcga cattaatatc
      361 atctttaatt gaaatatctt cagcaccaat tttcttctta tctttgttcg ttggtaataa
      421 tgccatttag tttaatctcc tttccaagag gtaggatatg attggccatt gagtcagcat
      481 atttaggtgt ataatcaaat ccttcaatat caccatgttg ttcatagtat tcaattcttt
      541 cttctaattc tgtaaagatg tctgcaaaaa tagcccacca tcttcgatct acataatcat
      601 ttaattggat accattctca ccatacattt ctaaccgatc cgagccttta acaatgcttt
      661 taaaaatgtt tgattcacca tacttatcaa ttgttttttg atagtttgcg tgttgagagg
      721 aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat ttgaagatga gactcttcaa
      781 aattcgtgac aatcggaact aaaactttat taataaaatc ttctgttcct tccattgcgt
      841 accgttttaa ttcttgcatt gcaataatat aatctgcagc agttaagaat gcacgaacca
      901 cattatctgt tgatggtggg cagtcgataa agatataatc gaaatttttt ctcaattctt
      961 ctaaaggttt aatgaaggcc aatgatctct tacgacgatc aggatatttt tcggacaagt
     1021 attcattaag ttcttcagat ccaggagagc cagccataat atataaatgt ggacttactt
     1081 gagtaatacc tgaaacccag tccccttttt tgattgctgc agacatggat cgatcaaagc
     1141 tagtaatttg atacgttgat ccaatgttgg aagtagtatt tctttgttgg tcataatcaa
     1201 ttagcaacac gtctttttca aaaagttgac tagaaacaat cgctaataaa tcattgtctg
     1261 tagtcttacc aatgccgcct tttctacctg taagcattac aacttttcct agtttatctg
     1321 atgttttaaa tgcagtccgt aagttttcca tagttccctc caatttccta tactaaatat
     1381 atttagtata cttcatatac ttaatatatt cgatatatct aatatactcc aaattgataa
     1441 gaattcagac tttataagga atatagtgga tatactaaat atatttgcta tattcagtat
     1501 atcagatata ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca atttagagtg
     1561 aaaagaggta aacttaattc tatagaaacc acagataatt cactatttac aaagtagcaa
     1621 aagcagtggt actctttaaa cagaaaaatt acataaaaaa aagagacgga aataattgat
     1681 tttagcagaa tcaattattt ctgaatcaca tggttaggac atgtgttcac gctcgtctca
     1741 agaatatgta agtagctacc aactacttat attaaaagtt gatcggatac cttttccgat
     1801 tgcctttatt ctaccaaaaa aaggcgagca actcaacaga agaaagttta tttagtgtac
     1861 ttaaattaaa tattcttaaa cactgaagag agaacacata ctatggctga gattaaagct
     1921 aaactgtgtt ctctttttgt ttgagaaagg acgttacaat cgatgttgag cgaaatttat
     1981 tcagctattc ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac catgaaacct
     2041 gcaggtcata ttgattccag taggagtggt gctatctttg cgtaccgtag caagcaatta
     2101 atgaatgttg ggcgtggtat ggtcattact tcggaagaag ctattttaga gaacgaaaag
     2161 aaactaacgc attggacacc taatacctat cgctttggta cttatgcgga tgattatcga
     2221 acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat
     2281 atcgattcaa aagagaacca tcaaggcgaa attattttag cctgtttaga tcaagtgggc
     2341 tacatgccaa cgctaatttt agaatcagat catggttatc aagtttattt tgtactaaag
     2401 acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt
     2461 tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg gagtggatct aggatgtaac
     2521 cacttcggaa ttgctcgatt cccacaagaa aataatattg tttttaaaga aatgaactat
     2581 cagtattctt ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca
     2641 aaagaagatc gagaacgaaa actaattgtc tttcctgaga aaaaagaaat tcgtcaagta
     2701 gatgaaccgt ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt
     2761 cttggtcgaa ataatgtgat tttcacgctt tctttagctt attactcttc aggttatgca
     2821 ttggatactt gcgaatacaa tatgttcaca ttcaacgaac ggttacagga accgctatct
     2881 gaagcagaat tagtaaagat tgtacgtagt gcttattctg gtaattatca agcagcaagc
     2941 agagaattca ttattgaatt gtgccagcaa tgggtttcac caaccttaaa aaataatgat
     3001 ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca aagaaaatat
     3061 agtcataaaa atgaatggaa agaagatcta ttggcttatc taagcgaaaa aagtcttgct
     3121 agtgagcctt atttgaacct gtcaaaaaaa gaattggctt cacaactaac gatgcccaag
     3181 cgaacacttg ataagatctt aaatgagtta cgagaggaga ataaagtctt ctacaaggta
     3241 acacgaggaa gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt
     3301 attcaactaa gtaaagaaaa gagactagat tattcaacaa ggatcacaga ggtatttgga
     3361 ctcaacgaga gcttagtaga agagacattt agacaattat caaccccctt gaaccctctt
     3421 aaagaagtga atctttttga agtagataca ggttgaaaaa taaaaggtgc aaattgccct
     3481 atacaatata tctatgatac ttctgtaggt gttgctccct ttgcctagct acgtgtaggt
     3541 ggtatactta taactgcgta tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa
     3601 aaaattatgt ctattcacat cctgttaatg tgtttatcat tggtcgtttg ggattatctg
     3661 tggaagtgtt ctgtgagctt tatggtttta agcaggggac tttgtcttct tgggttacca
     3721 gggaaaaaac agttgcttct ttgccgattg agtttcttca tgggttgtcc ttagcttctg
     3781 gggagactat ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag tatattgagt
     3841 ataaaatagc taacgagtta agaaaaagga agaagtatat ccagtagtag tttttattta
     3901 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
     3961 aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg
     4021 cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta
     4081 ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga
     4141 gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt
     4201 tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc
     4261 atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta
     4321 attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca
     4381 acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg acacatgtat
     4441 ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa acttattaaa
     4501 cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg ttatgagtac
     4561 aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca cacgagtatc
     4621 catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga aatgaaagca
     4681 atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct acgatatggc
     4741 atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata cattttttgg
     4801 aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat cgtttgaaaa
     4861 tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg
     4921 agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa acgggtacga
     4981 tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgttga
     5041 ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat tgattgatga
     5101 atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa
     5161 gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc tgtgcctatt
     5221 acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt gaaaggatag
     5281 tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct tttcgctatc
     5341 ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat gagaaaatcg
     5401 taagagaaat tgatcgaaga tgtgacgaa
//
LOCUS       DAJSVP010000121         5340 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSVP010000121 DAJSVP010000000
VERSION     DAJSVP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.126"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            20..2347
                     /locus_tag="ON449_002500"
     CDS             20..2347
                     /locus_tag="ON449_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3935022.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2473..2802
                     /locus_tag="ON449_002501"
     CDS             2473..2802
                     /locus_tag="ON449_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3935023.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2842..3846
                     /locus_tag="ON449_002502"
     CDS             2842..3846
                     /locus_tag="ON449_002502"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3935024.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3843..5272
                     /locus_tag="ON449_002503"
                     /pseudo
     CDS             3843..5272
                     /locus_tag="ON449_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1892 a         1027 c         1057 g         1364 t
ORIGIN      
        1 ttttttattg agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat
       61 atgtcaaaaa cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc
      121 cattatgatt tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt
      181 aaacgtttat acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc
      241 accctattct gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac
      301 ctcaaccaca tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa
      361 agcagcaggg gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc
      421 ggccactgcg aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa
      481 agatggagtc attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg
      541 cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg
      601 ctccacagta aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac
      661 cgctggaaat acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc
      721 ctcaacatgg ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca
      781 gttagcaaat gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg
      841 taagggaaat gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa
      901 tcggacagga actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg
      961 gactacatta tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac
     1021 gggctattct tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac
     1081 ggtgacctat acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca
     1141 gatgattgct ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac
     1201 gactaatacg cctactatca acggatatac ctttacagct gtttcagcga cagaaacaaa
     1261 gaaccaatca gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa
     1321 ggacgcaaat ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt
     1381 atcaatcaat gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa
     1441 atatgccttt aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga
     1501 tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac
     1561 tgcgcctact gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa
     1621 actagctgcc agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat
     1681 taagcaaatc agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg
     1741 tgtcccgtca atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca
     1801 agtggcactt agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa
     1861 tgctggcagc tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa
     1921 tcccaagttg tcatttactg atatagccaa cacatcagga acttatacca tctataaagc
     1981 taacgcagtg attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat
     2041 ttcggttgga aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct
     2101 aacagttaac agccggacag atattggatc acaaatagta acgattagtt taggcgaagg
     2161 aagtgtaaac aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc
     2221 gactctcttg cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact
     2281 gatctttatt ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca
     2341 gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta
     2401 ctggtctttt ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact
     2461 ttgcataatc gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata
     2521 aatatcccgg ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat
     2581 gatgtctcgg atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa
     2641 agaaaactag cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca
     2701 gtagacgagg aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt
     2761 gacgctgttt accgatttgc ggaaataaag acttcagatt aagtaaaaga aaaatcagca
     2821 ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac
     2881 ttgtatccaa agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag
     2941 aataagcaga cagattttgc agatggggat aaagttcctt atctcatcac cgtaacaatt
     3001 cctgagaaca tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta
     3061 tggctagaac cagaaacgat cgattttttg attggtggag acaagattca tacatttcac
     3121 acacaaaaaa caaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt
     3181 gctgggaaaa aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg
     3241 acatttgata acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc
     3301 atccaaacag gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca
     3361 gatgctcaat ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat
     3421 cattgggtag ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca
     3481 ttttctgtcc atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa
     3541 ggatatatat tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg
     3601 cttcatccta taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga
     3661 aaaacgccag ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt
     3721 ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt tagggttgtt gctagtcctt
     3781 tcaacaggga cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata
     3841 aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc
     3901 ctttcgttct cggcctagga actgcagagg tttctaggcc gtatcaccta caccagaaaa
     3961 tgttacggtt aatttgcata aattgaaatt cacttcagca ccagaaaatc aaataaacaa
     4021 cggaacggaa ttgacatttc cgaatagcga accgttgaac ggcgttgaat tcaacgtata
     4081 tgacatcact gctacgtact atccaagtaa agacacagca gttccagcag acgctacacc
     4141 cttcgcaagt gtaacgactt ctggtgaagg attagctaac ctgactcttc caggtaaatc
     4201 tgatgggaaa gatgctgtat acgtctttgt agaaacacca aagcccggtg tcgaaacgtc
     4261 gccaaatatc gtgttaagct taccctttta taacccgaat tcatcagacc ctgacaaacc
     4321 tttagacacg attcacctat atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca
     4381 gaaagctggg aacttttcag gggtagataa gttagcaggg gcgcaattcc agatcaaaaa
     4441 ttcagagaat caattcgtga ccggttttga tgaaaacact ggtgtcgctc tctatgatcc
     4501 tgctgagtca gcgttgacat tcacaactga tactgaagga tccatcagtg tcaatggatt
     4561 aatagacggc aattatactt tagttgaaac aaaagcacca gatggttatc aattaaccaa
     4621 tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat
     4681 catccatggc gcagatgaag ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa
     4741 tacaactacg gataacacga ttactgtaga aaatgtccaa aactacggtg atttccactt
     4801 catcaaacaa gatgtgaaca cgcaagaaaa attagccgat gcagaatttt ctattctaag
     4861 agcaaaagat ccaactgtta aattaatgaa aaaaatagca aatcttgcct caggagaata
     4921 cccatatgct tggtcagatg aagtcacagg ttctgactgg gaagaagtga aattgatttc
     4981 tgattcagaa gggaaatttt caatcgaagg gctgaaacaa ggggcatact gccttcaaga
     5041 aacaaaagca ccagaaggct acgcattacc taagaatgaa gctgcgtaca ctgaatttac
     5101 agtagatgat gatccaacaa cttctgatga aacaacgatc gataaccaac ctaaaggaat
     5161 cttgccttca actggtggta tggggatcat cgtattcatc ttggtaggta ctgctttagt
     5221 tggaggagca gtgatctact tcaaaaaacg ccatcaagaa acagaagctt aaactattgg
     5281 aaacaaaaat catacgataa ataaacgata gtgattcaaa tggggagaaa ggttgtgaca
//
LOCUS       DAJSVP010000122         5277 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSVP010000122 DAJSVP010000000
VERSION     DAJSVP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.127"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(27..1166)
                     /locus_tag="ON449_002504"
     CDS             complement(27..1166)
                     /locus_tag="ON449_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3935025.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1475..2188)
                     /locus_tag="ON449_002505"
     CDS             complement(1475..2188)
                     /locus_tag="ON449_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3935026.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2442..3143)
                     /gene="nagB"
                     /locus_tag="ON449_002506"
     CDS             complement(2442..3143)
                     /gene="nagB"
                     /locus_tag="ON449_002506"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3935027.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3377..4909
                     /locus_tag="ON449_002507"
     CDS             3377..4909
                     /locus_tag="ON449_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3935028.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(5100..>5277)
                     /locus_tag="ON449_002508"
     CDS             complement(5100..>5277)
                     /locus_tag="ON449_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3935029.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1732 a         1078 c          887 g         1580 t
ORIGIN      
        1 aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac ttttggactg
       61 tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg gatatttaat
      121 attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa tattcctgcg
      181 acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt cattgatttc
      241 ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa aatcgacaac
      301 gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc tggtaccgct
      361 ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac cgcaagcaac
      421 aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc aataatcaaa
      481 gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc ttttaccgtt
      541 tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc aataaatttg
      601 tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa aataaaaaat
      661 tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa tatgatattg
      721 aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt gaaattttta
      781 aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc atacattggc
      841 aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt catgacagtt
      901 aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc
      961 cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa accagcaaca
     1021 agcaatggta aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat attcggcatt
     1081 tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca gaaaaataat
     1141 tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat acttctattc
     1201 tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa
     1261 taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg agtagtgaca
     1321 aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg catcttcgta
     1381 ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg cttattactt
     1441 gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg actaaatgca
     1501 actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa gtgagccctt
     1561 ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg aattttacgt
     1621 caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta agaaaatctt
     1681 tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt acatggatca
     1741 cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc gcaatggaat
     1801 agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa gcggcagtcg
     1861 taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac
     1921 cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca gcaagctgtg
     1981 gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc aatgcaatga
     2041 tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga tttttatatt
     2101 gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt aaaataatct
     2161 ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata tatcatgtct
     2221 gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa
     2281 atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc ctcggtatta
     2341 ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa tactcaggtc
     2401 aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt acttgctgct
     2461 tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc tggcaaatga
     2521 ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc gccaaatgcc
     2581 atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac agcttttgtt
     2641 ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga ttctgttaag
     2701 tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca
     2761 ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat ttgatcatac
     2821 tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt ttctttgaat
     2881 ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc atctgaaccg
     2941 cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc caaatcgctt
     3001 gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc taatactttt
     3061 gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg
     3121 ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat agcccaatta
     3181 tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt tttttcattt
     3241 tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc aaacaatcca
     3301 tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc agttttttta
     3361 tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc aatttggcga
     3421 caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc ttgggctgat
     3481 tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg agatcttacc
     3541 agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt
     3601 ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg caaaagaata
     3661 cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct tcgatgattc
     3721 cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt tttatcaaat
     3781 cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag gtttagattc
     3841 actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa aactactttt
     3901 agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg atcagcatat
     3961 ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga tttcccatga
     4021 ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg gaaagattac
     4081 taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta
     4141 tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt atattcgaaa
     4201 atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat tagataaaat
     4261 cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc cattctcgcc
     4321 tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg atcgaccgct
     4381 acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc gaggcttcaa
     4441 cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa aaataagtgg
     4501 cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc tgtactggga
     4561 aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag cgactatcaa
     4621 agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa gtgaacctct
     4681 cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga caatgaacca
     4741 aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag
     4801 cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc atgaacaaga
     4861 attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa cgtttgattg
     4921 aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct tctgaataaa
     4981 tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta
     5041 tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc tcttttcgtc
     5101 tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca
     5161 catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg ctgccacatt
     5221 gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat aaataca
//
LOCUS       DAJSVP010000123         5203 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSVP010000123 DAJSVP010000000
VERSION     DAJSVP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.128"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..1998
                     /locus_tag="ON449_002509"
     CDS             64..1998
                     /locus_tag="ON449_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3935030.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2455..2871
                     /locus_tag="ON449_002510"
     CDS             2455..2871
                     /locus_tag="ON449_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3935031.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2885..3451
                     /locus_tag="ON449_002511"
     CDS             2885..3451
                     /locus_tag="ON449_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3935032.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3474..4460
                     /locus_tag="ON449_002512"
     CDS             3474..4460
                     /locus_tag="ON449_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3935033.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4615..>5203)
                     /gene="scm"
                     /locus_tag="ON449_002513"
     CDS             complement(4615..>5203)
                     /gene="scm"
                     /locus_tag="ON449_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3935034.1"
                     /translation="TDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSAS
                     SDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTT
                     SDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRAL
                     PKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1561 a          962 c         1155 g         1525 t
ORIGIN      
        1 tatcaaacta aaagaattat catatattca ttatatagat agtctataag gagcggatga
       61 tcaatggcaa agcaaaatat tgtcgttgta ggagcaggat atgccggtgt ttcagctaca
      121 aaatacttgg caaagaaact gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat
      181 tcgtaccata ctatgatgac cgagttgcat gaagtagctg gcggacgtgt agaaccagaa
      241 gcaatccaat acgatctgca gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca
      301 gacactgtaa tcggtatcga taaagaacga aaaatcgtca agaccaaatt aggttcctat
      361 ccttttgatc agctgatcct tggaatgggc ggagagccga atgactttgg tacacctggt
      421 gtcaaagcac acggattcac cctttggtca tttgaagatt ctgtaaggat tcgggaacat
      481 attgagaaga cagtagcaaa agctgcattg gaaccagatg atgaactgcg taaagccatg
      541 ctgactttcg ttgtctgtgg ttctggattt accggtattg aaatgattgg ggaactgatc
      601 gactggaaaa atcgcctagc caaagattat aaattagatc caaacgaaat cacactgatg
      661 gtcgtagagg caatgccgac gatcttgaat atgttggatc gaaatgatgc tgcaaaagct
      721 gaacgctatt tgcgtaagca caacgtggaa ttattattga atgcaccaat cgtagaagtg
      781 gctgctgatc atatcaaatt gaaagacgga cgcaacattc cgactcacac actgatttgg
      841 acagctggtg tcaaaggaac ttctgacgct gctgactttg gattagaatc agcacgcgga
      901 cagcggttga tcgccaatga atatatgcag gcaaaaggct acgaagataa aaacatctat
      961 atcattggtg atttagtctt ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt
     1021 caagctgctg aacaaacagg acacacagcc gcagcgaata tcgtggcttc gatcaaaggc
     1081 ggagaaaagc ataaatttaa aggaaactat caaggattta tggtttctat tggtgccaaa
     1141 tggggggtag ctaacctatt tgataagatc catttaagcg gatttttagc tatcgtaatg
     1201 aaacatatcg tgaatttgaa atacttcttt gatattcgtt ctggttatta tatgttccaa
     1261 tatttaatgc atgagttttt ccatatcaaa gatgatcgca acgtgactcg aggacattct
     1321 tctcgttacg gcaatgtctt atggagtgtg ccacttcgta ttttctatgg tttagtttgg
     1381 ttcgttgaag cttccaaaaa aatcattggg aatggtgaag tgttcaaacc aagtacttgg
     1441 tttggtgaag gatcatggtt cactggaaac agtgtgttcc cttggacatg ggaatacaat
     1501 gtaacagatg ttgccagtgg tgcttctgct gctggtgatg caaccacagc tgccagcgga
     1561 gcaggggcaa gtgccggaga agcagcgact caagctgcac attttggttt gagttatgca
     1621 tacggtgaag aaccaatggc tgtgattgag aagtcaccag actggttcac aaagatcatg
     1681 caagtgatca tgccaaacaa tgatgtagca atgttcttcc aaaaattcat ggtattcttt
     1741 gaaattgcct tggcattagc attgattgct ggattattca catggctatg cagtgcgaca
     1801 acgattgtat tggtgatcac gttctgctta tcaggcatgt tttactgggt aaacatttgg
     1861 ttcatttttg ttgctttcgc attgatgaac ggttccggac gtgcgattgg gttggatcgc
     1921 tgggtaattc catggctaca acgtacacta ggtaattggt ggtacggtag accaagttcc
     1981 cagtacggca agaaataaat agataaagaa cgttatttga atgcaagatt tcaatagaaa
     2041 ggggctgaga caaaccgtca cagcctcctt tttatgtaaa attaaaggaa taagatgccc
     2101 gagataagcc tccatgagca agacacaggt gtcacaacct ctgttacaac ctcttgttat
     2161 aaacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaacttgaa
     2221 aagtaatttt cgtaaattcc ttcttatttc ttgaagttgc ccacttctgt cacaacctct
     2281 tgttataaac ggtgttccac ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca
     2341 tggaaagctg ttttctattc ttcgttctta ttactcaaag caagacacag gtgtcacaac
     2401 ctcttgtttt tatgctaaaa ttgggtgagt taatctggaa aaaggagaac aaaaatgagc
     2461 cttaaagaat ttgctgaaaa aagtctgctc aaaccttggg atgtttttat tattttgctt
     2521 ttgactgttc tttctttttt accagtcatg atttttggtc ttcagcaaaa caccgcaaca
     2581 gcagaaaaag aagcggtact gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg
     2641 gctggagaag agagcaacac atatacttac aaagatgaac acggagatta taacttgatc
     2701 gagatagatg gcgatcgagt acggataaaa gaagcagatt gcgatgacca gatctgcgta
     2761 cgtcgaggct gggcaacgaa aaatggcgaa accatcgtct gtttgccaca taaattagtg
     2821 atcgaagtcc gttcaacaga tggaggtgac gaagacagtc tgatttatta gaccttcaca
     2881 caccatgagt aaattacaaa aaatgattta tatttcgcta cttgttgcac aaggagtgat
     2941 cattggttta ttggaaaata tgatccctta tcctttttct tttgcgcctg gtgcaaaact
     3001 aggcttagcc aatctaatca cgatcgttgc catttttacc atgaaaaaac gagacagctt
     3061 cttactactt tggctacgac tattcctgac tactttgcta ggcggaacga tttccacatt
     3121 cctttatagt atgagtggtg ctttgcttag ttattttgga atgttattgg tcaagcaatt
     3181 aggaccaaaa cgtgtcagta tcatcggaat tagtgcggcc ggcggtttca tgcataatgt
     3241 cggacagctt ttgatggctt ctttcattgc acaatcatgg acagtcatgt tgtacttgcc
     3301 agtcctttca gttttaggga ttttatcagg gattgcaatc gggatcgcag cgaattatct
     3361 tttacgacat gtgcagacac ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca
     3421 aaaatggagc ccaatgttta ccaaaaaata atcgtcacac aaggagtttt tttatgaagc
     3481 tacatccaat gtgggaaaac tatcctgctt taagtaaaga attgacagaa acactaaaac
     3541 ggatggaaga aactgtccgc ctgaaaaaca aacctgtcga acaagcaatc aaagaaacga
     3601 tccatgccgg cggaaagctg ttgcgtcctg cctatcagct attgttctca caatttggac
     3661 cagagcaaga tcgagacaaa gccatcgctt tggcagcttc tatcgaaatg ctccatacgg
     3721 ccacactgat ccatgatgat atcgttgatg aagcggatct gcgacgtggt cagcctaata
     3781 tccgtagtca gtttggtaat gtcgtttctg tctatgcagg agactattta tttgtctgct
     3841 gttttaaact attgtctgat tactccactt ccttgaaaag tctccaattg aattctcgca
     3901 gtatggaaaa agtattgaat ggcgaattag ggcagatgga tgatcgctat aattatgaac
     3961 tttctgtaaa agaatatttg gaaaatattt caggtaagac agctgaactg ttccaattaa
     4021 gttgttctgt tggcgctttt gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag
     4081 atatcggact ttctatcggg atggcctttc agattatcga tgatatttta gattatacaa
     4141 aagagagcca ggaaatcggc aaacctgtac tagaagacat gcgtcaaggg gtctattctt
     4201 taccgttgat ctactctttg caaaaaaaca aaccaaagct actaccttat ttagagaaga
     4261 aagcggcaat gacagaagaa gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag
     4321 gcgtggaaga agctaggaag ttagcggcct cttacacaga aaaggctttg aaggaaataa
     4381 agaaattacc tgccacctct cttcgaacaa aagagaatct cttttccctt acccaactga
     4441 ttttggatcg caaagactga atagggaaag gttcgatacg taaataaatt tctcagaagg
     4501 aaatctacgt gttttttgac aaattgaatt gtagttcttg tatttatgat aaaaagcaaa
     4561 aataaagagc aacgtccgtt agcgacgttg ctctttttta tcttgaatat ttttttacct
     4621 tttcgatttc ttataactca taaaagtgat tcttaaaccg attacaagta atataacgcc
     4681 tgcgagtgtt atccaattat tcgattgact tccagtttta ggaagtgctc tttttgattt
     4741 gtttccattg tcattgcttg ttttattagt taagttaaag ttcgtcctgt tatccgaact
     4801 tgtttttgag aaggagacac ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt
     4861 actatcgctt gaagttgatg aatctgagct atcacttgtg gtagaggatg tatctgtact
     4921 gtcacttgaa gctgacgtgt ccgtgctatc acttgtagtg gtagatgaat ctgagctatc
     4981 acttgtggta gaggtagtat ctgtactgtc acttgaagct gacgtgtccg tgctatcact
     5041 tgttgtggta gatgtatccg tactgtcgct tgaagctgac gtgtccgtgc tatcacttgt
     5101 ggtagaggta gtatctgtac tgtcacttga agctgatgtg tccgtgctat cacttgtggt
     5161 agaggtagta tctgtactgt cgcttgaagc tgacgtgtcc gtg
//
LOCUS       DAJSVP010000124         4963 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSVP010000124 DAJSVP010000000
VERSION     DAJSVP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.129"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ON449_002514"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ON449_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3935035.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ON449_002515"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ON449_002515"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3935036.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ON449_002516"
     CDS             2594..3379
                     /locus_tag="ON449_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3935037.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ON449_002517"
     CDS             3392..4243
                     /locus_tag="ON449_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3935038.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ON449_002518"
     CDS             complement(4367..4819)
                     /locus_tag="ON449_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935039.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1678 a          812 c         1037 g         1436 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttc
//
LOCUS       DAJSVP010000125         4933 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSVP010000125 DAJSVP010000000
VERSION     DAJSVP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.130"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            254..685
                     /locus_tag="ON449_002519"
     CDS             254..685
                     /locus_tag="ON449_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3935040.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            692..925
                     /locus_tag="ON449_002520"
     CDS             692..925
                     /locus_tag="ON449_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935041.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            1349..1549
                     /locus_tag="ON449_002521"
     CDS             1349..1549
                     /locus_tag="ON449_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3935042.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            1633..2826
                     /locus_tag="ON449_002522"
     CDS             1633..2826
                     /locus_tag="ON449_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3935043.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3004..3234
                     /locus_tag="ON449_002523"
                     /pseudo
     CDS             3004..3234
                     /locus_tag="ON449_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3362..4045
                     /locus_tag="ON449_002524"
     CDS             3362..4045
                     /locus_tag="ON449_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3935044.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4354..4427
                     /locus_tag="ON449_002525"
     tRNA            4354..4427
                     /locus_tag="ON449_002525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4388..4390,aa:Arg,seq:tct)
     gene            4586..>4933
                     /locus_tag="ON449_002526"
     CDS             4586..>4933
                     /locus_tag="ON449_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCR3935045.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLS"
BASE COUNT         1475 a         1006 c         1203 g         1249 t
ORIGIN      
        1 acagggctat tccggttatg agtatgtact ataccatgta aatcagcagc atagcaaagc
       61 attttctcgc ccggtaggtt tgcgacctgc cgggcgattt ttgtcgtctt ttgcggcccg
      121 gctgtcccca aaaaaatttt ttgagaaatt ttcaaaaagg aggtatttag cgggtagctg
      181 gcggccttgt caaagtcttg ttagcggaaa gggagagaac cacctaagtc ccccacagaa
      241 agggggtgag aagatggatg ctatcccccg cgactatgaa gcgcggtgca tgttcgatgc
      301 tttttgcaag acggtactgc gtaacgaggc caaaagctac ttggctgaaa tgaagcgccg
      361 ccgtgaccgt gaagtgtcac taagctcttt gtcacaggca gatttggaca agctctgtac
      421 agtagatcac taccccagcg acagctttac gttttcgtcg catggctatg acctacatat
      481 caacaatgag cttgttgccg aagcctttgc cgcgctgccc agcatggagc agagtatttt
      541 aatcctgcac tgtgttctgg atatggcaga tggtgagatc ggcggccttg tgggaatgtc
      601 ccggagtgcc gttcagcggc acaggacaaa taccttatcg gagctgcgga aacagctaaa
      661 ggcgttgatg ccgaagggag gttagacgcg gatgaagaac ccgaactaca agggcattga
      721 gcttttgccc ctttcggtga tcgaggccgc ccgcgctggg gatgccggag ccgtagagcg
      781 cgttttgcag tattacagcg gttacatgaa caagctgtgt acccggacgc tctatgacga
      841 aaacggctat ccgcatgtgt gtctggacga ggccatgaag cgccgtctgg aaatcaagct
      901 catccacgct atcgtcacga actgacgaac cggccccggc agcatgaaac ggcttaccct
      961 ttccccgttt cggtgttttc ccggtggcct gatcgcacct tgataaatat gcccgcaaga
     1021 taacggcggc gcatcatagg gcatacggac acattctgtc tgtgccgagc cggacggctg
     1081 gtgcgccatg atacttttcc ctacgggaaa agcggagcga ggaacaccac gccgcaatgt
     1141 gagcgtggcg gcttgcgggc gacgacgtac aggcaggact atgatactcc cttatagcca
     1201 cagtccgagc gttaaaagcg tcgcaggcaa tgggtagggc cgcgtgagaa ccacgcgggg
     1261 gtgaaagtcc cgtggagctg tgccagcagc cgtccgtcta tgcccctaaa tcacacaaac
     1321 ctttgaattt gtgaaagggg gctgcattat gaaccatgat gtgcctattt gggaaaaata
     1381 cacgctgacg attgaggaag cgtctaaata tttccgtatc ggagaaaaca aattgcgtaa
     1441 gctggccgag gaaaatccgg cctctggctg ggtcattatg aatggcaacc gcatccagat
     1501 caaacgcaag cagtttgaga aaataattga cactttggac acaatctaat agtgaaaaag
     1561 ggccgcagat atgttataat aatttataag catatatgcg gctctttccg tcatggaaag
     1621 gagcatgacg agatgtcaga aaaaagacga gacagcaaaa atcgcgtttt gcggtctgga
     1681 gagagccaga gaaaagacgg acgatatgct tacaaatatg ttgatacctt tggaaaaccg
     1741 cagtttgttt actcatggaa gttagtgcct acggataaga ccccggcagg taaacgggag
     1801 gatatttcac tccgggaaaa ggagaaagaa atccagaaag accttgacga cgggatagac
     1861 accatcggca agaaaatgac cgtctgccag ctctacgcca agcagatcag gcaccggggc
     1921 aatgtgaagc acaacaccaa actgggccgg gaacggctga tgcgtattct ggaacaagat
     1981 cggctgggga gctgcccgat tgacagtgtg aagctgtccg acgcaaagga atgggcgctt
     2041 cggatgaaag aaaagggcct ttcttataag accatcaata acgacaagcg ttccctgaaa
     2101 gcggcttttt acaccgccat acaggacgat tgcatccgaa aaaacccgtt tgacttccag
     2161 cttagtgacg ttctggacga cgatacggaa ccaaaggtgc ctcttacacc agcacaggaa
     2221 gaaagttttc tgtcctttat tcaaggtgat aaggtttacc aaaagcatta tgacgcaatc
     2281 gttattctgc tggggacagg gcttcgcatt tcggaactgt gcggactgac ggacaaagat
     2341 ttggattttg aaaaccgggt aatcatcgtg agccaccagc ttttgagaaa taccggcgtc
     2401 ggttactata ttgacgaacc caagacccaa agcggcgtca gaaaaatccc catgaatgag
     2461 gaagtgtatc aggcgttcca gcgagtgata aaaaaccgca aaggtgcaaa gccgttcatt
     2521 attgacgggt atgccaattt cctgtttctc aaacagaacg gctatcccat gacggcggta
     2581 gattatggcg ggatgtttgg caggcttgtg aagaagtaca acaagagcca tgaagaagcg
     2641 ttgccgaaaa cgacgacgcc ccatgctatg cgtcatacat tctgcacccg gttagccaat
     2701 gcgggaatga accccaaagc cttgcagtac attatgggac attccaatat caccatgacg
     2761 ctgaactatt atgcacacgc cacattcgat tccgcaaggg cagaaatgga acggctggca
     2821 gcctgacaca aggccggttt tactactcca tttactactt ttgaggggga aatggtgaga
     2881 ggtaataaga acatttgtaa gtatctaaca atatcaaaaa tgccggaaaa gcccggaaac
     2941 acgggctata ccgacattca agaacttcta aaaagataat ctaaaatccc atataatttt
     3001 tcctcaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
     3061 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
     3121 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
     3181 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
     3241 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
     3301 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
     3361 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
     3421 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
     3481 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
     3541 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
     3601 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
     3661 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
     3721 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
     3781 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
     3841 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
     3901 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     3961 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     4021 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     4081 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     4141 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     4201 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     4261 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     4321 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     4381 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
     4441 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
     4501 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
     4561 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
     4621 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
     4681 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
     4741 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
     4801 ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt
     4861 atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt
     4921 ggcattgctt tct
//
LOCUS       DAJSVP010000126         4888 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSVP010000126 DAJSVP010000000
VERSION     DAJSVP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.131"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            54..1001
                     /locus_tag="ON449_002527"
     CDS             54..1001
                     /locus_tag="ON449_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR3935046.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1298..1846)
                     /locus_tag="ON449_002528"
     CDS             complement(1298..1846)
                     /locus_tag="ON449_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935047.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1847..2704)
                     /locus_tag="ON449_002529"
     CDS             complement(1847..2704)
                     /locus_tag="ON449_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3935048.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2990..3277)
                     /locus_tag="ON449_002530"
     CDS             complement(2990..3277)
                     /locus_tag="ON449_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3935049.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3267..3596)
                     /locus_tag="ON449_002531"
     CDS             complement(3267..3596)
                     /locus_tag="ON449_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3935050.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3673..3945)
                     /locus_tag="ON449_002532"
     CDS             complement(3673..3945)
                     /locus_tag="ON449_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3935051.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3945..4208)
                     /locus_tag="ON449_002533"
     CDS             complement(3945..4208)
                     /locus_tag="ON449_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3935052.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4314..4730)
                     /locus_tag="ON449_002534"
     CDS             complement(4314..4730)
                     /locus_tag="ON449_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935053.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4736..>4888)
                     /locus_tag="ON449_002535"
     CDS             complement(4736..>4888)
                     /locus_tag="ON449_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3935054.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1462 a          919 c          681 g         1826 t
ORIGIN      
        1 ttttttggta taatgagaaa aaatatctat ttgaaaaagt taggagctat actatgcatc
       61 tggaattctc tgactatgaa tcactccttg taaaaatttg tgaagactat tatctcgagg
      121 atatcaatat tagtgaaatt tctaaaaaat atgggatcac cagatataaa gctttaaaat
      181 atattgaaga tgcacgtgaa aaacaattag ttagtatttc aattaattct ccgtatgcta
      241 ggaattatga gctagaagaa atcttcaaaa attactttaa aacgaatatc tatatattaa
      301 aaagtagtga agacttatca caacacgatt tattattttc aaaatttgct gccgattaca
      361 ttcaagaatt aattaagaaa gccaatgtgg ttgcactatc ttggggagat accgtttaca
      421 aagtgattga tcagtttaaa atgggtgctc gtgaagaatt aatttttact caattcattg
      481 gcgaaatcgg aaaatataat tccttagctg gctccacacg attagttcaa aaagctgctg
      541 aacgttatga gtcttcttat ttgactctca gtattccttt atatgtcatc aatgaaactg
      601 cacgtgaact aatggctcta gaaccttctt tagctaatac cttagcaacc gcttcgcatg
      661 cagatatgtt aatttctgga attggcacct tatcctctat tgattctgtc gaaccctgga
      721 acaagcatcg taaaatccta tttccaagca tgacggatac tgttggattt ctttacggaa
      781 gacccttcaa tatcaatggt gaattcatca aagcatctga ggataagact tttggtcttt
      841 ctttaaaaga aattttcgat attccaaaac gaattggtat ttgtaaaagt aagttcaaag
      901 cggagagttg tttaggcgcc ttgaatggta atttttttac agatttattt ttggatgaaa
      961 aaactgcttg gaaaatactt tctttgataa aaaaagagtg agtcatgtaa aatcctccaa
     1021 aaaatttaga tttttggtac tacttttttg gagggcagta caatgattca ctctttttat
     1081 tggatatcaa aatccctttt tttgagaagc ttgtcttgat attttttgaa cttttccaag
     1141 ccatactcca ttacttttca agatttaatt tctttgtatt cactgaataa tttccacgtt
     1201 ttttcgttaa tgttacttct tattaatttt attgtacttc tcatgcaata ggatcatata
     1261 tccttctaaa tcttttttat gctataaaaa gggggttcta tctaacttta ttattcattc
     1321 cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta gttcttatta
     1381 tttctccacg gtcatctaat tctggaacaa ttggatattt ttctctgaac aagtctagtt
     1441 ctttcaccac gtcattaaac aaatcttgct tatcttcttg cgtaagattc tgtttaatat
     1501 actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca tacttttcta
     1561 ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc ttggtggaat
     1621 aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct ctactctgtc
     1681 tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat tgcttatttt
     1741 cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt attttggcga
     1801 gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac tccacctccg
     1861 cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat tcgtggcttt
     1921 tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata cctgatccgt
     1981 cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt tccccataaa
     2041 tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc aacatgaaag
     2101 gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag tccatatact
     2161 caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg acgattgccc
     2221 agtccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg acatctaaat
     2281 agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat ttgaattggg
     2341 ataatgaatc attatattgg tctttgatcc aatcatcata catttcaaag gacatatctg
     2401 ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt ggaagattgt
     2461 ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct ccgcctcctt
     2521 cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt ccttgcatat
     2581 ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac cctaacattt
     2641 gcgcgcacgt acttttacct acgccacctt taaaattacc aattacatat ttcttcgcta
     2701 ccacgttcct acctcttttc ttttagcaat attcatttga tactattata acatatttat
     2761 ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat aagtaaaagg
     2821 tagtacataa ataaggaaaa gttgaaatat attgatttat agctttcctc aaagtaatga
     2881 cgttatttaa tttatcgaga agcagagaat actcgcgact ttagtcatgt agacgaatca
     2941 gattttcgat aaaaaagaag ccgtccttct taagaacgac aacaaatcac tagattaatt
     3001 ttttcaactg ttcataaaag ttttcatgcg ttccacataa caatactgga ataaccttgt
     3061 tttctataat ctcgtaagct actctatatg ttgtgcccgc atatttgaac ccactagacc
     3121 aaatacctga taaatcccca gttttttgtt cacccttata tggatcaatc gcaatttcat
     3181 cataaattaa attcaaaaat ttttctttca atggcttctc ttttagcttt tttaaatact
     3241 taatgacatg ttttgaagga acgatttcat aagccaattt ctgttgccaa ctcctcttta
     3301 gtcattactt tagaattagt aagtgtatct tcagcaatat ctctaaagga agaatgcatt
     3361 ttgttttttc tattcttaaa ttctacaaga atctcatctt tatcgtagcc ttctgcaatt
     3421 atatcagaca aaatatcttc gctaaaatca taaaattctt tttgtggttc aacaaattca
     3481 taaagaatac cattctctac caactttgct tcaacttcga ctccattagg aacattaaaa
     3541 tctttaggta ctgtcaacat gattgaattg ccttgttttc ttgtttttgt aatcatttaa
     3601 atccctcatt tctaacagta tgtaattact tagtaaatac attataccac gagataatgt
     3661 atttacaagt aattactgtg actcgtaatg cgtccttgct gaataaattt caaccacgtg
     3721 ttcatcttca tctaccttat aaacaacccg gtgttgtcta ttcaatcttc tagaatacct
     3781 accttcatat gtaggtttta acttctcaaa tgattgggta gggagatagg gatcttcctt
     3841 taaagtctga ataaccttct caaattgagg tttcaaatta gactgtttga ttttctttaa
     3901 atcagcttta gccgagtttt taatcatcac agaatagtta ttcattataa gttatcccaa
     3961 tcaatttcat caacattagt aaatccacta tcatcttttt ctcgcttacg aacaacaccc
     4021 aaagtgccta tttgctcaag taatagcgtt tctttgattg ctttcaaatc atccaaacca
     4081 ataagcaccg cattattttc actgcgctca ctaatgattt caatttccgt atgattctca
     4141 tttacatctt tcaacaactt ataaaaattt ttccgtgctg agctaggatt taagactgtt
     4201 tttgccatat ctactccccc tctaatttat atctccattc taacgtactt aattaagtac
     4261 gtaaattcga ttgttttgtt taaattggag taccaaaaca gcattgtaaa gtgttatccg
     4321 tttcctttat ttttattaac aagttttatc acaggcttca tatatttgat tataacttca
     4381 tcttctacgt agatatctgt tatcattacg acatctgtac tatttcgatt gagtactttc
     4441 acttgatctc cagcattgat tttccctcta aatcttttag gaatgaacca actatacata
     4501 tgattagcat caccaaaatg tgcttttatc gcattctcag gatataactt attcaatagt
     4561 tcattctttt taattttcaa aacttcaaat ctatacttat catcatctgc taatctaaat
     4621 aaatcttcta aacgcatgtt ttctaactgt aagttttgaa aatcttccgg tataaatttt
     4681 cggttctgtt gcgaagtttg aaaatcaaac gcgccctctc tgaactccaa atatcttagg
     4741 ctgttattcc cattaatacc ttgatttcag tagacaccga aaagctgaag agcgttccat
     4801 ttcttcggtt ctttttatat attcctcgaa tggtctccat gcccttaatc gtggaagagg
     4861 ctgtacggag actttgataa aatttatt
//
LOCUS       DAJSVP010000127         4870 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSVP010000127 DAJSVP010000000
VERSION     DAJSVP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.132"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..787)
                     /locus_tag="ON449_002536"
     CDS             complement(53..787)
                     /locus_tag="ON449_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3935055.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ON449_002537"
     CDS             1114..1524
                     /locus_tag="ON449_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3935056.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ON449_002538"
     CDS             1517..2218
                     /locus_tag="ON449_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3935057.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ON449_002539"
     CDS             2218..3804
                     /locus_tag="ON449_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935058.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ON449_002540"
     CDS             3823..4044
                     /locus_tag="ON449_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3935059.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ON449_002541"
     CDS             4041..4802
                     /locus_tag="ON449_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3935060.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
BASE COUNT         1522 a          877 c         1002 g         1469 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa
//
LOCUS       DAJSVP010000128         4797 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSVP010000128 DAJSVP010000000
VERSION     DAJSVP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.133"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ON449_002542"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ON449_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3935061.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ON449_002543"
     CDS             complement(2065..3063)
                     /locus_tag="ON449_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3935062.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ON449_002544"
     CDS             complement(3289..3942)
                     /locus_tag="ON449_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3935063.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ON449_002545"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ON449_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3935064.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1407 a         1007 c          744 g         1639 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat tttttta
//
LOCUS       DAJSVP010000129         4792 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSVP010000129 DAJSVP010000000
VERSION     DAJSVP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.134"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            121..315
                     /locus_tag="ON449_002546"
     CDS             121..315
                     /locus_tag="ON449_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935065.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            305..658
                     /gene="tnpB"
                     /locus_tag="ON449_002547"
     CDS             305..658
                     /gene="tnpB"
                     /locus_tag="ON449_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3935066.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            760..2307
                     /locus_tag="ON449_002548"
     CDS             760..2307
                     /locus_tag="ON449_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3935067.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2384..2821)
                     /locus_tag="ON449_002549"
                     /pseudo
     CDS             complement(2384..2821)
                     /locus_tag="ON449_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3135..3557
                     /locus_tag="ON449_002550"
     CDS             3135..3557
                     /locus_tag="ON449_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935068.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            3567..3770
                     /locus_tag="ON449_002551"
     CDS             3567..3770
                     /locus_tag="ON449_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935069.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            3981..4565
                     /locus_tag="ON449_002552"
     CDS             3981..4565
                     /locus_tag="ON449_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3935070.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1593 a          793 c          931 g         1475 t
ORIGIN      
        1 tgtatctgca ctatagagtt ttggacgacc tttataaagt aagcgcccca tagaacagta
       61 ccttactaac aaatgcacct caagatatac ttgagctatc aagaagtctg gaggttttct
      121 atgtataaat taaatgagat tgttcctgtc caattaggaa acgaaaagag tgcaccagaa
      181 aaaggtaaac ccgcggctgc caagttagcg atgcgcatca aaagagagaa tggttctgaa
      241 gttttgatct ataacggtat taacaattat attcttcaag ctattttgaa agaactgttg
      301 tccaatgctt tatgatttac ttcaaaccaa acatatttat attgtgtgcg gaaaaacaga
      361 tttgcgaaaa ggaattgatg gtcttgccag tcttattcaa caagagtatc aactcgagct
      421 ttacgaagat gccgtttttt tattttgcgg gaaccgtcaa gaccgtttca aacttctcta
      481 ttgggacggt gatggttttc ttctctgtta taagcggata gaaaacggca agttaaaatg
      541 gcctcggacg aaagacgaag ttcgaacctt aacgaaccaa caggttcgat ggttgttaga
      601 aggattaagt atcgatcaac ctcgagctat tttgccagga aaaaaaggag ttttttaagc
      661 tggggggtta acacaaaaaa tgtgttgaac ccctatttct tgtgcgtttc ttgtggtatt
      721 ctgtaacaaa atcagttcga ttcaaggtgg tgaaaaattt tggaaactac agatacgttg
      781 cttcagttgc ttcaagaagc ccataaaacc aatcaagcac aacaacaaac cattcagaat
      841 cttacaactg aaatccaact attgaatgaa aaggtcaatt atttaaccaa caaattattt
      901 ggacgctcta aagagactct ctttgaggaa acgaatgggc aattgaatct ttttagtgat
      961 gaagaaattt ctgtaagtgt tccagaagca gctgccacca ttattccagt aaaaggacat
     1021 caacgagttg taggaacgaa agcagacaaa atcaaacact tacctataac agagaaagaa
     1081 catctccttc ctttggaaga acaattttgt gaacattgcg gttcacagat gaaagatatc
     1141 ggacggacaa aagtacgaga agaaattcgt ttccatcagg ctatgttgga ttgtctcacc
     1201 cattatcaac atacctattg ttgtaaaagc tgcgaaaaag aaggactttc ttcttttaaa
     1261 aaagcgatcg ttcctaaacc tttgatttct aatagcttag gttccaatag tttagtggcg
     1321 gaaacgatcc ggatgaaatt tggtcagaaa gttcctgcct atcggcaaga gaactattgg
     1381 aaacaaacac atgggttgga tatttcgaga gataatatca ccaattggca tattaaagca
     1441 gtgcaaaacg cgttagaccc tttaggagaa cgattaagag tctatcttaa tcaagaagag
     1501 attctacacg gggatgaaac aagttatcgt gtgattgaaa gtgccaaaac agatacctat
     1561 tactggcagt tttgtacagg aaaagacagt cagcatccca tcgtttatta tcaccacgac
     1621 gaaagtcgtg ctggagatgt tccgaaaact tttctcaagg agtttacagg gtatttacac
     1681 tgtgacggct atagcgggta taatgccgtg gaaagtgtac gacttgtcta ttgtttcgcg
     1741 catgttcggc ggaaattttt cgaagcaatt ccaaaaggaa agaaaaacac ggatattccc
     1801 gcagctcaag cagtcaaaca attggataaa tggtttgtcc tagaaaagaa atggaaagac
     1861 ttttctcctg agaagcgatt aagctgtcgg caacaagaac tgcgtccatt atttattgct
     1921 ttttatgagt ggatggcaac gatagatcct gttgcaaaat ccaagttgga tgcagccgtt
     1981 caatatgcgt gtaaactacg aagtggtttt gaacctattt ttgaagatgg tcgtttagaa
     2041 ctcacaaata atcgtgcgga gcgaaatatc aaagagctgg tgataggacg gaaaaattgg
     2101 ttgcattcca caagtcttga aggagcacga acttcaggaa tcattctgag tgtatataaa
     2161 acagcagaac ttaacggatt gaatcctgta aagtatctag agtttctatt tgataagata
     2221 ccaaatcttc ccgtactttc agcagaaact ttagatcaac tgttgccttg gaacaaggat
     2281 gtacaacaac atttttctcg taactaaaaa cgccaaacat tcccgattct attctatagg
     2341 tcgggttatt tgacgtacag gtactgttct atggggcgct tactttataa actccatttg
     2401 ctttcgcaat tttaatgcct tgcgcctggc gtcgtttaga ttcggtacgc tcttgttcag
     2461 cgatcatggc caaaatttgg atgatcagat ccttaatgaa tttatccagg agagggttgc
     2521 caattgcttc agccatgatc ggtagactgg tgatgattag ctgtacgttt ttcttcttta
     2581 ggtaattgac tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca
     2641 caataaaaat gtcttgatct cggacgaaat ccaatgcttc ttggaaaaca ggtcgatgag
     2701 taatcgttgc gcccgattgt ttctcgatga aaattctcac tgcaccgaat tttttcattt
     2761 cttcaatctg gcgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca
     2821 aaatttttac ctactttctg caactattta aaatacaccc taatcataca cgataaaacc
     2881 agatttttca atgttgtggt cgtgtctagt tagggtatac cttatataga caaaaactaa
     2941 tgatggagga aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa
     3001 atgtcaatca agaaaattta aacgattatg acattaaaaa tatattttac gaattttcta
     3061 gtatataact tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt
     3121 cgaggtgata gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa
     3181 ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg
     3241 tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc
     3301 caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag
     3361 cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt
     3421 ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa
     3481 aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa
     3541 ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc
     3601 aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc
     3661 agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg
     3721 aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa
     3781 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
     3841 ttctggacac ttcctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
     3901 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
     3961 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
     4021 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
     4081 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
     4141 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
     4201 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
     4261 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
     4321 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
     4381 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
     4441 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
     4501 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
     4561 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag
     4621 tatgagaatt ttttgtaagt gtctactgat aacttattat caaatgttgt aaggatttta
     4681 ttcatttgtt aatattaaag aacggaactc caaaaaagtc cgtactctaa tttgcagtct
     4741 ctcactcttt tagagtgaga gtttttatta gattttcaaa ttaattagta aa
//
LOCUS       DAJSVP010000130         4771 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSVP010000130 DAJSVP010000000
VERSION     DAJSVP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.135"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            109..651
                     /locus_tag="ON449_002553"
     CDS             109..651
                     /locus_tag="ON449_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3935071.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            668..1753
                     /locus_tag="ON449_002554"
     CDS             668..1753
                     /locus_tag="ON449_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3935072.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            1765..2571
                     /locus_tag="ON449_002555"
     CDS             1765..2571
                     /locus_tag="ON449_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3935073.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            2572..3399
                     /locus_tag="ON449_002556"
     CDS             2572..3399
                     /locus_tag="ON449_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3935074.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            3396..4469
                     /locus_tag="ON449_002557"
     CDS             3396..4469
                     /locus_tag="ON449_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3935075.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         1513 a          778 c         1022 g         1458 t
ORIGIN      
        1 caaaaaaaat tttacaaagg gttgcctttt tcaataaatt tcgtatacta atacgagtgg
       61 tagtaaacag gaagtttact attagaaaac ttcgattcgg aggaaattat ggaaattggt
      121 gagaagttaa gaaatttgcg catccaaaag aatttgacac aagaagaact aggcgaacgc
      181 acagatttgt caaaagggta tatttcccag ttggaacgag atttaagttc accatcaatg
      241 gaaacattct tttctattct tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa
      301 gaagcagcga atctgcaagt ggtttattca aaagaagatc atacggttta tttcgatgaa
      361 gaaaacgggt atgaattgga atggctcgtg gctgattcga atgaaaaaga aatggagcct
      421 gtcttgctga cttttgaagg tgcaggagaa tataagacgt ttgaaccttc attatctgaa
      481 acgtttattt ttgtattaga aggggaatta gagttaagct taggagagac ggtctatgtg
      541 gcaaaagcag gacagtcgat ctattatcaa gcgacaaaac aacatcaatt aaggaatcat
      601 ctcaaaaaac gaacaaaagt attgattgtt gcaacgcaat catatttgta atataggggg
      661 taaaaacgtg gggaaaccaa tcatttcatt tgatcaagta gtgaaacgct atgatgatga
      721 aacagtatta aaaaaagtaa gttttgagat cgaacaagga aaattttata cactgcttgg
      781 tccttccggc tgtggtaaaa caacgatcct acgtattata gctggattca ccgaagctac
      841 agaaggggac atctattttg aagggaaacg gttgaatgat ttgccagcta ataagcgaca
      901 agtaaataca gtcttccaag attatgcact ttttcctcat atgaatgttt ttgataacgt
      961 tgcttttgga ttgaagatca agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga
     1021 tgctttgcgt atggtccagc ttcctggata cgaaaaacgg gagattagtg aaatgtctgg
     1081 tggtcaaaga cagcgtgtag cgattgcacg agcaattgta aatgaaccga aagtattgct
     1141 attagatgaa ccgttatcgg cgcttgattt gaaactacgt acagatatgc agtatgaact
     1201 gcgggatctg caacaacgat taggaatcac gtttattttt gttactcatg accaagaaga
     1261 agcgttggca atgagtgatg agatttttgt catgaataaa gggaaaatcg tccaaagtgg
     1321 tacacccgta gatatttatg atgagccaat caatcatttc gttgctgatt ttgttgggga
     1381 gagcaatatc gtggacggtg taatgatcga agataatcta gttgagtttg ttggtaagca
     1441 atttgaatgt gtcgatggcg ggatgcgtct aaatgaacca gttgaagttg ttttacgccc
     1501 agaggatttg acgatcacga caccagataa aggaaaacta gtcgtaacgg tcgatactca
     1561 gctgtttcgt ggtgtccatt atgaaatcat ctgttatgat gaacagcaaa atgaatggat
     1621 ggtccattca acgaaaaaag ctaaagaagg aagcaaagta ggattggcct ttgagccaga
     1681 agatatccat gtcatgcgtt tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag
     1741 ctacgaagaa taggggggac aagaatgaaa acaatgcgca ggatctattc cgtcccttat
     1801 attttgtggc tcggtttatt cgtgatcgcg cctgtattaa tgatcattta tcaatctttt
     1861 tttgatatga atgggcaatt cacgctgaat aattatctat cttattttac gtcggggaca
     1921 tcgctttcta tgacgatcaa ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg
     1981 atcatcagtt acccgactgc ttatttttta acaaaactga agcacaagca attatggctg
     2041 atgctgatta ttttgcctac atgggtgaat ctgctgctta aagcttacgc atttattggg
     2101 attttcagtg tgcacggcag cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa
     2161 cagatattat tcacagactt cagttttctg ttcgtggcaa cgtatatcga gattccattt
     2221 atgattatgc caattttcaa tgcattggaa gagatgaatc cttctttgat cagtgctagt
     2281 agagatttag gcgcgaatag ctttgagacc ttccgacgag tgatttttcc gttatcatta
     2341 aatggtgtga aaagtggtgt ccaagcagta tttattcctt ctctttcttt attcatgctg
     2401 actcgtctga ttggcggaaa tcgggtgatc actttaggaa cggctatcga acagcatttc
     2461 ttggttacac aaaactgggg gatgggttct acaatcggtg tgattttaat cattgctatg
     2521 ttcatcgtga tgctcctgac aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa
     2581 tttaaatggt cgtatcttta tttagggatc gtctttttat tcttatatgc accgatcttc
     2641 tatctgattt tttattcctt caatgaagga ggaacgatga acagctttac aggatttact
     2701 tgggaaaatt atgaagctgt ttttgaagat acacgtttga tcattatcgt attgaatacg
     2761 ttcatgctgg ctttcttatc tgcattgatc gccacgatca tcgggacatt tggggctatg
     2821 ggaatctatt acacgaaaaa acgacgtgca cggacagcgt tgttgagcat gaataacatc
     2881 ttattagtct ctcctgatgt tattatcggt gctagtttct tgattttctt tacactacta
     2941 ggattcggat taggttttca gagtgtattg ctttcccata ttgctttcag cattccaatc
     3001 gtcgtcttga tggtcttgcc aaaattgcag gaaatgaacg attcaatggt cgatgcggct
     3061 agagaccttg gtgctaataa tttccaagta gtcaaaaata tcattttgcc ttatctttct
     3121 ccaggaatca tagctggata ttttatggca tttacctatt ctcttgatga ttttgcggtt
     3181 acatttttcg tcacaggaaa tggctttacg acgctctcag tagagatcta ttctcgtgca
     3241 agacaaggaa tcagtttaga aatcaatgcg ctaagtgcgt tagttttctt gttctccatg
     3301 atattggtca ttggatacta ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa
     3361 aacagaagag tacgcgaggg ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt
     3421 taggtattct catcattatt gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa
     3481 gtggcatgag tggagcaaag gtacttacca tctataactg gggagattat atcgatccta
     3541 gtttgatcac gaaatttgaa aaagaatatg gatacaaagt gaattatgaa acatttgatt
     3601 ctaatgaagc gatgttcaca aaaatccagc aaggtgggac aaactacgac atcgctatac
     3661 caagtgaata tatgatccaa aaaatgatca aagaaaaact agtacttcca ttagatcatt
     3721 cgaagatcaa aggattaaat aatattgatc cacgattttt agacttggat tttgatccag
     3781 ataatcagta ttcgattcca tatttttggg gaactttagg gatcgtatat aacgataaat
     3841 tctttgatgc aaatcaaatt aagcattggg atgatttgtg gaagccggaa ttaaaagaca
     3901 gtttgatgct gattgacggt gcacgagaag ttatggggct ttctttgaat agtttaggct
     3961 attctttgaa tagtaagaat atggcacagc tgacagaagc agcaaataaa ttgaatcatt
     4021 taacaccaaa tgtcaaagca atcgtggcag atgagatcaa aatgtatatg atcaatgaag
     4081 aagcctccgt ggcagtgaca ttttctggcg aagctgccga tatgatgtgg gaaaatgaac
     4141 atttacatta tgtgatccca ccagaaggat ccaatctttg gtttgataat atcgttatgc
     4201 caaaaaccgt gaaaaataaa gaaggtgcct atgatttcat caactttatg ctagaaccgg
     4261 aaaatgcggc acaaaatgca gagtatattg gttattctac accaaatgaa gaagcaaaag
     4321 cgttattgcc aaaagacatt tcgtcggacg aacaattcta tccatctgat gacacgatct
     4381 cccatcttga agtctacaaa gatctaggat cgaaatacct aggcatctat aatgacctat
     4441 tcttagaatt caagatgtat aggagataat ggcaaaactc agtaagaatg gggacttatc
     4501 aacgttctaa agagctaaaa atcgtccttt ttctatctga gttttccaat cttctaccaa
     4561 aagcggatga aacgccgctt tttttgtgtt attttatttt ttctaccacg tttttctacc
     4621 agagaaaagt ggacaaaacc attgtaaaat agggcccgag acgggctagt aaatctaaga
     4681 ttgaaaaagt tacagaagga taaagtaatt ggaaactatg caaatatact taacccttct
     4741 gtaatctatt aataaaacta atatatgaat c
//
LOCUS       DAJSVP010000131         4626 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSVP010000131 DAJSVP010000000
VERSION     DAJSVP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.136"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(61..2424)
                     /locus_tag="ON449_002558"
     CDS             complement(61..2424)
                     /locus_tag="ON449_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCR3935076.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2741..3928
                     /locus_tag="ON449_002559"
     CDS             2741..3928
                     /locus_tag="ON449_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3935077.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            4346..4567
                     /locus_tag="ON449_002560"
     CDS             4346..4567
                     /locus_tag="ON449_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3935078.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT         1317 a          912 c          889 g         1508 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgattacgt
     2641 ccatataatt gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa
     2701 tgttttttgc agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac
     2761 ttttgaaagc gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa
     2821 aacacttatg actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca
     2881 ggcaaatgcc tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg
     2941 ttcgtttaca actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa
     3001 gtttagtcct actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat
     3061 gattgaaatg gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct
     3121 aacggatggt gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc
     3181 atttgttttt gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg
     3241 tgatgccctg tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat
     3301 tggtatcggc attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg
     3361 cgaatcggaa gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg
     3421 tgtaaaactg gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt
     3481 tttaggtgcg agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct
     3541 gccaaagaaa gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga
     3601 acttgaattg acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa
     3661 attaagtgct gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc
     3721 ttttcctgaa aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga
     3781 agagattcgc cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg
     3841 gataattgga acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata
     3901 tttagaattt aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg
     3961 aggagagaca tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg
     4021 catggcagtt aggttcacaa cgcaaaacat tgtgcttttg atgaggttga acttttacac
     4081 aagattgtgg acttgacttt catttcgctc ctcatcatgc ataatttgct gaatgacgct
     4141 aggccagtta acacattgtt ggaaaactgg ggctggatcg agcaattcct ttcctgattg
     4201 atccgtttta tgtaaaagtc tctgttatct ttgtcaatat gtgaattggt atcccagcta
     4261 ctatgctaat ttctacaggg attatccaaa atttggatcg tgaccaaatc gaagcggcgt
     4321 aatcggaaat atcaataact tcaacgtgat ttttctttta actgtcaacg gaccgacaaa
     4381 ctcaaacttt tatggtgcag gttccactga tcttctagtt acttggctat acctgactgt
     4441 cactaaaata gattacaatc tagcttccgt tatcggtatc ttgatcttta tctatatgca
     4501 aaaatttata tactatagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt
     4561 tggttagctg acacgctacc agactaaagt catggaactc ttaggtaata cgtcttaatg
     4621 aaaaca
//
LOCUS       DAJSVP010000132         4560 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSVP010000132 DAJSVP010000000
VERSION     DAJSVP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.137"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            581..1492
                     /locus_tag="ON449_002561"
     CDS             581..1492
                     /locus_tag="ON449_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR3935079.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1979..2245
                     /locus_tag="ON449_002562"
     CDS             1979..2245
                     /locus_tag="ON449_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935080.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2317..2658
                     /locus_tag="ON449_002563"
     CDS             2317..2658
                     /locus_tag="ON449_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935081.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2713..3132
                     /gene="ssb"
                     /locus_tag="ON449_002564"
     CDS             2713..3132
                     /gene="ssb"
                     /locus_tag="ON449_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3935082.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3209..3505
                     /locus_tag="ON449_002565"
     CDS             3209..3505
                     /locus_tag="ON449_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935083.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3520..3951
                     /locus_tag="ON449_002566"
     CDS             3520..3951
                     /locus_tag="ON449_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3935084.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4133..4438
                     /locus_tag="ON449_002567"
     CDS             4133..4438
                     /locus_tag="ON449_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935085.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1680 a          719 c          966 g         1195 t
ORIGIN      
        1 ttgaccattt tctctcaacg tggaaaaacc gccacttatg agagaaaatg gtcaaaaacc
       61 taagcgggaa gtctccgaat aacataccaa gcgccgacag ccaacagata aagaatacac
      121 tactaaaaat agtagtagca taaaatttaa aagggaaaaa atcaacccct accccagtct
      181 tggggtctca aaactaactc ccccctaacc ccctctttca gcctcttttg aaagaggggg
      241 agaatcaacc ccacggccaa cgccccagtg ttcttggggc tgtccagaac agaaacttgc
      301 gagagagtgg gggtaaaaca agtgtagagg aaacttgaag acttgttcga acagaaactt
      361 gcgagagagc cggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacagaaac
      421 ttgcgagaga gttggagtaa aacaagtgta gaggaaactt gaagacttgt tcgaacacaa
      481 acttacaaga gaggggagta aagcagttgc agaggaatca ggaagatttg ttcgaacaga
      541 aacttgcaag aaagcattat taaattgaag gtgatgaaat atgaagtatt tattgcatgg
      601 ctattttatt gattattcta ctcgcaatag tagtacttta gcgattctat gtttgcttca
      661 tgaattgaga gtggttacta gaaaacagct atacaaacta ttgaattttg acagatcaat
      721 tagtaaacaa ggagtaacga atgctttagt gagattatat gaaaaacagt taatcgatcg
      781 aaaaaaatat ggaacagaaa tgtattatta tttgacgaaa gagggacatc aaagcatagg
      841 tgggtattat acgttgccga aagttcctga atataatttg aatcaccatt tacaaattaa
      901 caattatttg attaaaggaa tggcattggc aaaagaccac ccacatttaa aacggattat
      961 ttcagaaaga cgtaccgtct atgaaacgaa ggatttaaat cctaagacaa aaggaagaaa
     1021 attttttgtg ccagatttta tttttcgttt tcgagacaaa gaaaatcatg agatcaattg
     1081 gtcctttgaa attgagctaa cgttgaaaac acgtcgtcgc taccgtgaag cgatttttcc
     1141 gaaatatatt cgtgagttgc gaacacgtcc tgaatcacat ttaatttatg tgacacctag
     1201 tggcgcaatt caagaagaac ttcatgcgtt taagcgtatt tttttcaagc gcgaaactga
     1261 aaaagatgcg agtcaacaag aattgtttga gcggttgcat attttttcag ctttagagtt
     1321 tgaagaggga ttaaaagcat tattagagaa tgatccagca gtcaattggc ggacagtaga
     1381 acgagaaaaa atttatccga atggcgctgt tcaaagtcgt ttgaatttat ttgaagaatc
     1441 aactgaaaat gaggaaaaac caaaagtgaa gtttacgcct gaagtattgt aaaagagggt
     1501 gttcgcttcg ctcttttttt gtgccagttt gctggcaccg ttcagacgca agcgtctgaa
     1561 ccggctcgtt tttaggaagg actaacttgt ttgatttatc cgttgtcaaa gaagtggacg
     1621 ttcgagagtc cctaatcgta tagcagtcat tcggcgagta taacctaaaa cataacctgc
     1681 caattgccaa gggtaaaatg cacgctgcgc gcccttggca atcgtcagaa catgttttcg
     1741 agccggttat gccgaacgac cgctatacga aaagggcctc taaacagaag tccactttga
     1801 tccttgacaa ctgaaaccca aaatcaaaca agcaggcctt cgtctaaaaa cgagccgaat
     1861 gaagaccttg ggggttgcac ccccaaaccc ccgtgccccc gacacactac cgaaaaggtg
     1921 ggggtgggtg gcgaaaaaat caaataaaac attctattca agaaaggaag aaacaaccat
     1981 gacaactaaa aacgagcaac tgaaagaaca ggcaagaaaa gagttgcaag aaagaggttt
     2041 gattattgag gggagctttg aaggagattt tgaaacctat attggttgtt atgcaagacc
     2101 gataaacaaa cctacagcct tagacccaat gaacgaacaa gaagtaaaag aacaagaaaa
     2161 acatgcagtg aatggttttc cgcaagattt cactgaatgg tatgagtggg aaattaacaa
     2221 cgggaagctt gaaaacttct tataaaagtc agatgacgtg caaagagaat gattctcttt
     2281 gtacggtaaa aatataaaga aagaaggaaa aagaaaatgg caaaaaacac accttatcaa
     2341 gtaggagaca ttttttatag tagttggggc tacgaacaaa cgaacgtgac tttttggcaa
     2401 attgtgaagt taacagaaaa aacagcatgg tttcagccta ttcaaaagaa aacggtcgag
     2461 cattattcgt ccatgagtgg aaaagttgtt ccaatgaaag acaagtttat agagtatcct
     2521 ttagcaaaaa ctaaagacag tattgtaaaa aaacgaattg atccagaaac ctatcctgat
     2581 atgttatgtg gttatcgtag ttgggacacg tttcatagat ataaaaatca agctgtttat
     2641 gaaagtagtt attattaagg ggatagtttc ccctttcaaa aataataaat agaagaatgg
     2701 agagaaaaaa tcatgatgaa ttcagtgaat ttagtaggac ggttaacaaa agaagtggat
     2761 ttaaaataca caccaaaagg caatgcgaca ggcacattta ttttagctgt taatcgaaat
     2821 tatacgaatg caaacggcga gcgtgaagca gattatatcc gctgtgtcat ttggcggaaa
     2881 gctgcggaaa ctttagcaaa atatactgga aaaggaagtt tgattggcat taatggacgt
     2941 cttcaaacga ggagctatca gaatcagcaa ggacagacca tgtatgttac ggaagttcta
     3001 gtgacagatt tctatttgtt agagagtaaa gaaatcaatg agcaacggtc aaaatcaatg
     3061 gcgaataatc aaaataattc ttcaattcca ggaaatgaga ttcaaatttc agatagttat
     3121 ttgccctttt aagtgaaaaa taattttgtt ctatacctga tttcttcagg tatagaacaa
     3181 aaaataaata gagatgaggc gattttttat gaaaatcatt gacagttacg atcaacgaac
     3241 catgcaatat ttgggctcgt ttgattatac agatgaaaat attattaatt atgttgcaag
     3301 tttacctcct tatcaaagcg ttcgcttggt agattttgca agtgatgaat tggtattaac
     3361 aacgatgggc aactttttag attatgtcgg agacaaaaaa tggttggaaa aaattttgtc
     3421 taaattgatt gcgaaacaaa caggagaaga acccataaat gcgattcaat tgttcaatcg
     3481 atatgaacaa gaaaacaatg attaaagagg agagaaaaaa tgaaaagatt aaaacgaagt
     3541 atttttattg tgctagcatt gattgatttt gttgggacag gttgtgaagc aattgacgtt
     3601 caaaaagctc ccttatcgga aataaaacaa gcaataacaa aacaagatga agaacaaccg
     3661 ctaaatacac aagtagaaat agtgaatcaa aataaaccga gtttttctga agaagatttt
     3721 tcgttagaaa atggaaccag gcaatttttt tcagatttgg atcgtttaaa tcgggtaggt
     3781 gtagcaagtg cacttttgca taaaagcagg atgccaacag aagaacgaga ccgattatac
     3841 attaagccaa ctggctggaa acaaaagaaa atgaagaatg gcgattatct ttataatcgt
     3901 tgtcatttga ttggctatca acgtgcgaca agtttcccca taagtatgta atacgaaacg
     3961 ggaggattaa tcttaagatt aatcacaact aacctatttg acagttggaa tgaaagctat
     4021 catgtctgct gaatcaactg gtctgatact cctacatgca ataaagtcta ctaggaagtt
     4081 attgtatgaa gctaggtaaa ggcggcagaa caatttctaa gtgtggaatc atatgaagtt
     4141 ggatatgccg ggacgctata aattactcag ggtgagactg tttttaatta aactgacgaa
     4201 cttgcgaatg tacgggtcta taaccgactt atattcgaaa gatataagat tgcgaatgca
     4261 aagaacatta tcgaaaagta agaatgttgg tcatgaaatt cgagatggaa gactagcggt
     4321 gtatctgtca agagttggta aaacatgttt gattgattca gatgagagtg ggaatgcgac
     4381 aaaacgtttt acagaagaaa tagagcaact agcagaactg aatatacgat atttttaaat
     4441 actttagcgg agaataaaaa aatcctctac agaaaactac ggagggcaac atgtgctatt
     4501 gtttcaacaa tacattttta atttttacca taacaaaaaa gtacttgaaa aaaaacagaa
//
LOCUS       DAJSVP010000133         4444 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSVP010000133 DAJSVP010000000
VERSION     DAJSVP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.138"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ON449_002568"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ON449_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3935086.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ON449_002569"
     CDS             complement(2017..2919)
                     /locus_tag="ON449_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3935087.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ON449_002570"
     CDS             complement(2916..3608)
                     /locus_tag="ON449_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3935088.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ON449_002571"
     CDS             complement(3651..4292)
                     /locus_tag="ON449_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3935089.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          946 c          768 g         1354 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gatt
//
LOCUS       DAJSVP010000134         4442 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSVP010000134 DAJSVP010000000
VERSION     DAJSVP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.139"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            630..1220
                     /locus_tag="ON449_002572"
     CDS             630..1220
                     /locus_tag="ON449_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HCR3935090.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1247..2557
                     /locus_tag="ON449_002573"
     CDS             1247..2557
                     /locus_tag="ON449_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3935091.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2554..3273
                     /locus_tag="ON449_002574"
     CDS             2554..3273
                     /locus_tag="ON449_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3935092.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3288..4373
                     /locus_tag="ON449_002575"
     CDS             3288..4373
                     /locus_tag="ON449_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3935093.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT         1581 a          491 c          861 g         1509 t
ORIGIN      
        1 gaaggcgatt taagctatat attagaaaat gagatatttg taaacaaaac aaggagactt
       61 ttgttgggac gctgtcgtac ttgtattcaa aaggcagtta gatatgctag ttttaataaa
      121 gaatttgatt taaactccta tttgtctaag atacttatga acgatgagtt gattgcctgt
      181 attcactcat acccatataa acaaaatcca attaaacaac gaatatttaa ctatttttta
      241 ctaaaccaat ccattattgg ggttaaaaca ttagcatatt taagcggaat gggtagaaat
      301 aggagatttt aatcataaaa aactgtacta tagtgaattt ttggagattt atattccagt
      361 tatatattaa tgattgtgat acttgtacta ttgtaaaaat tcgttaggtt taaataactg
      421 gaaatatcgg aaaatgggca ttatacatca cggacatccc ttcttttgga aaaaatgtta
      481 cgatttttat aagtaatatt gtatccggtg gaatcaacga tgaagataac atatccatcg
      541 ttgtagattc agtagcattg aatgatgctc ttgttgcaag aaatccgaat aaaattgtta
      601 agtataaatt gaattaatag gagatatgaa tggaaattag cagtaaagaa tatttaaaag
      661 cggttcttcg tatggaaaag aatctatata tagaatctaa aggacaacac acaaaattac
      721 taaaaataat gaaatcacat ccggactaca acgcttggcg gtatactcgt cgaatgagaa
      781 ttacatctta ttattattca aagagaaaga aaaatatcat ttatgcgatt atgtatattg
      841 tgtcttgcag aagaatgaat agtttgggaa gaaaatgtgg tatagagtgt ggtgaaaatg
      901 tatttgatga aggtgtaaga ttttaccata cacaaggaat tgttattaat ggtaacgcca
      961 taataggaaa aaattgttac ttatatggga ataactgtat tggaaatgat ggaatcgatc
     1021 ctaagtgtcc tgtaattgga aataatgtac gtgtatgcgt gggtgcaaag attattggta
     1081 atgtaaaaat agctaacaat gtagttgttg cggcaggagc tattgttatc aaagattgtc
     1141 tagagaatgg tgcaattctt gcaggtgtac cggcaaaaat aatagggtat gttaatttaa
     1201 caaaagtatt tttagaataa tttttagcga aagaaggtga ataacgatgc gtatttcaat
     1261 aaaaagatgg tcaggagcag attggacatt gttcacgttg gcattttttg caatctccat
     1321 agttcttgta tatactttta acgccccaag agcaatttta tatttgggag atataattaa
     1381 tatctttgtg ttattaaatg cacttagctt tagacgcaga aaaggaatat atgctagcaa
     1441 aaatgagatg ctgatagttt tgtttttact agtttctatt tgtagctcag ttattaattt
     1501 agaaaatata ggattgatta tctgggggat acgaaataac actcgtatgt tcatagtata
     1561 tataaattgt gcaacatatt taacaattaa agattactat aaagtattcg ctataattga
     1621 aaaattattc tggatttcac tgccactttg tttaattgaa agattcatgg tcacctatcc
     1681 aactggaaca attgtaggag atatgatagg tggagttttt tggaattatt caggttgtaa
     1741 tatgccattg aatttaattt tgtgtgtgac agttactgtt gttacaatcg gatatttcaa
     1801 gggagaaaaa aatttcatcc attttatttt atgtgtttgt gcagcacttt ttatgtctgc
     1861 attagctgaa ttgaaagttt ttattcctga attatttata atatttgttg caatagtatt
     1921 cactttaaaa gttgatttta aagtagtgat caagatatta tcatgcgtaa tacttgtgtc
     1981 gatagtatta tcctttttta taacaatatt tattacattg aacgataatg gacaaagcta
     2041 tgctgataat ttcacaattc aaggtctttt agaatatgcg tcaagagatt ctggatatga
     2101 tggtgttggt gatttaaatc gaataactgg tatctctaga gtagtagatg aaatatttaa
     2161 taataatttt agattagtcc taatgggagt aggcattggc aatgcagagt ttaccaattt
     2221 ttttgtcagt gagttttata aatcgtttgg taatcttcat tatcagtatt ttcatcatat
     2281 ttggttattt atagaagttg gaatattagg agtttcgata ttcctatttt ttataataaa
     2341 gacatttttc aatgcaaaaa gatcttttat gaaggataaa gatgcaagtc ttgttaaagt
     2401 tattgcaatt atgtcggtat tcttttttgt gtataacaca acacttaggg gtgaaacaac
     2461 ggggccactg atttatatga ttttagccat gcctgaagtt gtgaaaagga aagagcatta
     2521 ttatagtgaa aaaaatacaa aaggagagat attatgattc ctaagaagat acattactgt
     2581 tggttcgggg gaaatccact tccgaaaaaa gatagaaagt gcatagaaag ctggaaaaag
     2641 tattgccctg attatgagat tattgaatgg aatgaatcaa attatgattt aaataagaac
     2701 cgttatatgg ccgatgcata taaagaacgt aaatggggtt ttgttccaga ttatgcaaga
     2761 tttgatatta tttataatca tggtggattt tatttagata cagatgttga gctaattaag
     2821 tcgttagata tacttcgtga aaatcatgcc tatatgggat ttgaagggga aatctgggtt
     2881 aatggcggta ttggttttgg tgcagaagaa ggacattctg ttattaaagg tttaagggat
     2941 atttacgatt cattaagttt ttatcaaaat gatggtacat tgaacttgaa accgagtccg
     3001 tattatatta cagattatct tgaatctatt ggattggttc gcgataacag cgaacaaata
     3061 ataaatggcc tgcatatcta tcctacggat tattttgctg ccaaggatta tgatactgga
     3121 aaaatttata aaactagtaa tacaataagc attcatcaat ataatgcatc ttggaaaaca
     3181 cctaaacaaa aatttgttta tagattaaaa tcaacaattg gacaaaaaaa atttaacagg
     3241 ttacttgcac tgaaaaaatt tttacactta taaaggagaa aagtactttg aagaaaattt
     3301 gtttcattag tggtgtaatt tctcgttctg ggggaactga aagagtaggg agtattattg
     3361 ccaatagctt gtctgattta ggttatgatg tcactatttt aagcttttgg aatagtggag
     3421 agccttattt tgggttagat aagacgatta aggttgatta tatgttaaat cctaaaacgg
     3481 agggaaaatt atatagaact tatttatatc cggtttggaa attgcacaag tatatatgca
     3541 aaaatgaatt tgatatagta attgatattg atactttgtt gtctttgtat actacaaggg
     3601 caattaaagg aacaaagtgc aaattgattt cttgggaaca ttttaattac tggacaatgg
     3661 aaatgctgaa agaaaagaag agattcaagg ctaaaaaatt gataaaaaaa tatgcaaaga
     3721 ggcttgtggt tctcacagaa gaagatcgtc aaaaacatat tgatgaatat agtttatcag
     3781 aagattttgt tataactatg ccaaatccgt gtatatctaa tatcgagtac gaatatcatt
     3841 atgataaaaa gagatttctt gcagtagggc gattagtgga agagaaaaat tttgctgaca
     3901 tgataagtgc ttggaaaatt gtagaaaaaa aagtagatga ttgggatctt atcattgtag
     3961 gaaaaggtga attagaaaaa gatcttcgcc agcagataaa agatttaaat ctgcaaagag
     4021 taactatagc agggcattca gaccatgtgg aggaatatta taaatctgca tcttgttatg
     4081 tgcttagttc tgtttatgaa ggatttccta tggttatatt agaagcacag tcttatggat
     4141 taccagttat ttcttatgat tgtaaaactg gtccgagaga tttggtacat cataatttta
     4201 atgggatgtt ggttgaagat aagaatatag accaattagc taagagcatg ataatgttta
     4261 ctaagaatac tgatttagca atgagaatgt cactgaacgc atataataat gtacaaaaat
     4321 ttaatttgaa agaaattaca aaacaatggg ttgctttgat tgaaaatgta tagttttttg
     4381 tggttgtcca aacatgaaaa ttagcgtaat taatcaaaga gctagaagaa acctattaca
     4441 tt
//
LOCUS       DAJSVP010000135         4393 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSVP010000135 DAJSVP010000000
VERSION     DAJSVP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.140"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            283..522
                     /locus_tag="ON449_002576"
     CDS             283..522
                     /locus_tag="ON449_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935094.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ON449_002577"
     CDS             complement(884..1144)
                     /locus_tag="ON449_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935095.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ON449_002578"
     CDS             complement(1329..1826)
                     /locus_tag="ON449_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3935096.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ON449_002579"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ON449_002579"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3935097.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ON449_002580"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ON449_002580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3935098.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1276 a          931 c          687 g         1499 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aat
//
LOCUS       DAJSVP010000136         4359 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSVP010000136 DAJSVP010000000
VERSION     DAJSVP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.141"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            70..1371
                     /locus_tag="ON449_002581"
     CDS             70..1371
                     /locus_tag="ON449_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3935099.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1398..1658
                     /locus_tag="ON449_002582"
     CDS             1398..1658
                     /locus_tag="ON449_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3935100.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2657..3892)
                     /locus_tag="ON449_002583"
     CDS             complement(2657..3892)
                     /locus_tag="ON449_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3935101.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1274 a          694 c          895 g         1496 t
ORIGIN      
        1 tagttatgaa cattctttgc gttgtcatgc aatgaatggt tcatatttta ttgaaaagga
       61 gtggttttag tggaaaagct gatgaaatgg gtcgaagaga aacttgtccc accaatggca
      121 aaaattggta cacagcgtca tttattagca attcgtaatg gggttgtttc aacattgtca
      181 ttgattttga ttgggacatt ttttatggtc tttatcaatt taccgtttcc tggatggaat
      241 gaatttattg ccccttattc ggcaacgatt gttttaccct ttaggatgac gatgggattg
      301 atggctattt atgcaacttt tgttatgggt tcggatctag ctaaatcata tggattagat
      361 agtgtgacag gtggtatatt atctttagga acgttcttta tgttacaagt tcctgtaaac
      421 gttttaactc ctgaagaggc acctcttggc tgggtcttac ctatgagttc tttgggagca
      481 tctgggatgt ttgcggggat cttatctatg atttttgcag tggaagtata tcgtttcttt
      541 aaaaatcgaa atattactat caaaatgcca gaacaagtgc cacctgctgt tgcccgtagt
      601 tttgaggcat tgattccagg tgcagtcatt ttaacaatta cttggctgat tcgaagcgtc
      661 gcgggctttg atgtaaacgc tgccttatta tcgttattcg agcctttaac caatatttta
      721 gggaataatt tgcttggtgt tttactacca atgtttttga tccatttgtt gtggtcattt
      781 ggtatccatg gcatgagtat tgtaggggct gttgttcgtc caatgtggct gatcatgttg
      841 gatgagaatg caaaggcatt ggcagatggt acagctgcca cccaattacc ctatatcgct
      901 cctgaacagt tttatcaatg gacggtaaca attggtggtg caggagcaac gatcgtggta
      961 tctgttttgt ttatattctt ctgtaaatca aaattcttga aagaggttgg tcgtttttct
     1021 attattcctg gtatcttcaa tatcaatgag cccataattt ttggagctcc tatgatgttg
     1081 aatccttaca tgttcatccc gttcaactta gttcctttag ttttgactat tatcagttat
     1141 tttgcagtca aattagaaat ggtcaataga tttactgttt tgccaacatg gacattacca
     1201 gctccaatcg gcggttatct ttcagcagga aatgactggc gtgttgttat cttgatcatg
     1261 atcaacacat tgatagcatt tattatctat tatccatttg taaaagctta tgataaaaag
     1321 atgctgtcag atgaacaaag tcgtggagaa aacgaagtcc agactgtcta agtttggttt
     1381 ttagaaggtg ggaaacaatg tattggctga ttttattttt tgtttttatc tttttattaa
     1441 cggctagtca ccttatattg aatatgctag ctacatatca tattcaaatc aatcgatgga
     1501 tttgggcact ggcatcattt ttgatcgtga ttcttccaaa gatcattgta ccgcatatga
     1561 atgttttgtt ttcatggggg acatatgtac tttgtggaat ttttgctatc aattttatga
     1621 tcgagcagca tagatggttt gtcacatcga agctttaaat gttctaaaag tggttgtaac
     1681 agaagtggtt agcttcaaga aataagaaga aatttacgaa aattgcttct taaatttttg
     1741 tgaaatgttg gcttattttt cgtttggttg aacttattac cgcagatgca agcttttgac
     1801 caccgtttat tcggaatatg ggactgtgac aaggcttttg tcacagccct tttttacata
     1861 aagattatta ttgttttaac tgttttggca gaaagaagga ggaaaaaatg gaaattttat
     1921 cagcaatgga tttagaatta gtagatctac taccaaagtg atcaagtcaa atatagattt
     1981 gggagcttac aaccatatag taggggctgc agtatggtgt atgaatggta atatgtatga
     2041 tgtgagtgtt tattcaaatc atgaaacgtg tatagaaata attgtgctgg gaatagcaat
     2101 cagtcatggt gaacgtgact tttagtgtat cgttgctgac gacatagaac attctgacat
     2161 gatctactca atatgtgggg attgctggca gtttatatag gagtatgcat cggattgcga
     2221 gatcattgtc tcaacagaag agggaggttg attatcaaag ataaagtggt aaatcctaca
     2281 tttgatttag taattgttga ggggactagc ggcacaaatc tctacaaagc gatgcttcta
     2341 gatctatatg acacatttta agaaaatgta tttgcctaat tattatggtc tttcttttga
     2401 agaaacgttg agatgatcca accagagaaa ggtgtcaaag aatttgacta agaataaatg
     2461 gcagacgcta cctatgctca tttggatagc gtcagatctt ttattttaag gatgattttg
     2521 tgttttttag acaccgtaat ttaagtaact ctaacttttc aatccactat ttactttttt
     2581 tgtattttca ctgtctgggt ctcgaatagc gaaattactg atgagtctct attaaaaaag
     2641 ctttttctac ttgaaactat attgggtggt tgctcaggaa ggtttcgatt tcttgaattg
     2701 cttgttgtgg accatttatt ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct
     2761 tgggtgaaga aacttcgcgc agagcaatct gcagttttct ttcagtcact ggttttgtga
     2821 gggcgattgc gtttccgtat tttacacaat agtcaatatt acagctttgc tcaaattgca
     2881 tccccacacc gataaacggt ttgccggatt cacaggcagt ttggacggtc ccttccccgc
     2941 cgtggataaa agaaaaatca atgaattcat gcaaaagatg actagacaga tattctgtta
     3001 ttaaaagatc ttctgagtca gattgaatgt catcaagcag atagtcaata ggtgcaataa
     3061 ccgctagatc cggttgtttt tgtaagaaga acaaaatttt gcgtaacata cgcggattgc
     3121 ctgaactacc cattgcgaaa tagataatac gttttctttc ttgttgcttt cgctgtataa
     3181 aagttagcac ttcttcaggc aaggaagtac tgagcttagc aaaaattgga ccaacgattc
     3241 gataattttc cggcagctgt tcttcttcca aaaatagttt aggcgttgtg atgagattta
     3301 gatcagcatc catcatttgt aaacttgttt ttggtaaagg aagcatttct ttttgtgcag
     3361 cgtgtttgaa ggcattgggg atccatcggg tatttactat acgctttttt gtccagtgcc
     3421 aaaggggatt gcttaaaggg ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg
     3481 attgaaaaaa aggttcactc aaagcatagg gtttaacata tactaaagga atctttttat
     3541 tgcgggccga taaaaaaatt gtcacattcg aacctgtcac aatcagtcga ggctcaaact
     3601 tagcaatcag actctgttca ctagtgatac gagaggcaac catttctttg gtaaaagggt
     3661 ttttaagtgt acgcaactgg tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga
     3721 tcggcgctag taaatgatac tcaaagccac tttctgtgat caaaggagca aaagttttag
     3781 aaaagccaaa gaatacacag taataacggt ctttcatagc tttggcaacc tcaatcattc
     3841 gtgttgtctc agctaaattg aatactgcag gagcaaaaag aattgtcttc atgaaatcct
     3901 ccttattgga atgagtctat cccttcgttt cttactagta tactaaaaaa cacagcgttc
     3961 gagtagccta tgtatatttt gaatattaaa ctgtctctcg ctgttttcac aacttctaaa
     4021 tttaaatttt gtaatcaagt tgaaactcca attttcaaaa aaacacgcaa caattttttg
     4081 atcgatttat atgttgtgcc agataaatac gagaagttgt gtcattaaaa ataaacatga
     4141 gaacataaaa aaataaaata attatattaa ttgataggtg tgctgtcgtc attatacaca
     4201 cgtaagaaag aaatagttat gtttaaaaat attcttagac tttctcgcca tgacaatttt
     4261 cactatgtgt tataactgta aacgataatt attcagataa cacaaaaaga aatcaaggag
     4321 cagatacgcc agttgtttaa tttgaatatt attttaaaa
//
LOCUS       DAJSVP010000137         4239 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSVP010000137 DAJSVP010000000
VERSION     DAJSVP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.142"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            107..808
                     /locus_tag="ON449_002584"
     CDS             107..808
                     /locus_tag="ON449_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3935102.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="ON449_002585"
     CDS             complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="ON449_002585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3935103.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="ON449_002586"
     CDS             complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="ON449_002586"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3935104.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="ON449_002587"
     CDS             complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="ON449_002587"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3935105.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3390..4112)
                     /gene="tsaB"
                     /locus_tag="ON449_002588"
     CDS             complement(3390..4112)
                     /gene="tsaB"
                     /locus_tag="ON449_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3935106.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1256 a          894 c          735 g         1354 t
ORIGIN      
        1 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
       61 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      121 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      181 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      241 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      301 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      361 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      421 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      481 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      541 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      601 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      661 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      721 acaactacgt gattccggac tgttagatta tgacaaaaag cgttttactt tctgtaagac
      781 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgatatt
      841 tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat
      901 cactgggcaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg
      961 caaaaaatac tgatagatta actagaggaa gcgtcttgtc ctcatccgac ctagattatt
     1021 cgatattcct cgacaataaa cgattaatca gtgagggcaa tcagaggaac ggataatgat
     1081 gaataagctg taaaaaatag ggctttgaca aaagcgtgtc acagccctta tttcgattaa
     1141 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1201 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1261 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1321 agccgttttt cctgggctgt tcttattact tgatgcaggg cagatttttc acaacctctt
     1381 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1441 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1501 gggggaatca ctagttctac ctcagggagt ttagttgcca aagcatcctg caatccttca
     1561 cgcaaccctt gattggccgc tacacctcca gctacaatca gttgcttgac tggataattt
     1621 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1681 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1741 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgt
     1801 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1861 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     1921 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     1981 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     2041 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2101 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2161 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2221 gtgatttgtt ctacgtgatg gcggctagct acttcaggaa ctacgccacc aaaacgttta
     2281 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2341 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2401 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2461 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2521 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2581 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2641 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2701 cttcaactaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2761 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2821 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     2881 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     2941 ttgcttgaaa acctaacttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     3001 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3061 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3121 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3181 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3241 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3301 attctcttag tacgtaacga cgatcgattt gttcgattgt tttttctgta taaatcattt
     3361 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3421 ggtgtgtgcg tctccaacca tttttcttct gcttctaccc gttttaaata gcgaggaaga
     3481 aaagcttgtt tctctgcaac tggttcagct aaagcaccta attgagccac agttacacca
     3541 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3601 tcatagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3661 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacaat ttctaatcta
     3721 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3781 acaataattt tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3841 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     3901 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     3961 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     4021 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4081 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4141 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4201 gtacaatgat acatgattat ctgctggcgg gcaaatttt
//
LOCUS       DAJSVP010000138         4115 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSVP010000138 DAJSVP010000000
VERSION     DAJSVP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.143"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            47..1471
                     /locus_tag="ON449_002589"
     CDS             47..1471
                     /locus_tag="ON449_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3935107.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1652..2425)
                     /locus_tag="ON449_002590"
     CDS             complement(1652..2425)
                     /locus_tag="ON449_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3935108.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2422..3327)
                     /locus_tag="ON449_002591"
     CDS             complement(2422..3327)
                     /locus_tag="ON449_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3935109.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3481..3801)
                     /locus_tag="ON449_002592"
     CDS             complement(3481..3801)
                     /locus_tag="ON449_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3935110.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1238 a          758 c          821 g         1298 t
ORIGIN      
        1 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
       61 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
      121 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
      181 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
      241 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
      301 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
      361 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
      421 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
      481 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
      541 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
      601 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
      661 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
      721 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
      781 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
      841 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
      901 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
      961 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
     1021 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
     1081 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
     1141 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
     1201 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
     1261 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
     1321 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
     1381 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
     1441 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
     1501 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
     1561 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
     1621 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
     1681 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
     1741 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
     1801 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
     1861 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat
     1921 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
     1981 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
     2041 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
     2101 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
     2161 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
     2221 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
     2281 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
     2341 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
     2401 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
     2461 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
     2521 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
     2581 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
     2641 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
     2701 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
     2761 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
     2821 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
     2881 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
     2941 gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc
     3001 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
     3061 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
     3121 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
     3181 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
     3241 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
     3301 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
     3361 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
     3421 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
     3481 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
     3541 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
     3601 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
     3661 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
     3721 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
     3781 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
     3841 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
     3901 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
     3961 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
     4021 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
     4081 agaccttatt tctatatatt atcgggcaat aaatt
//
LOCUS       DAJSVP010000139         4022 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSVP010000139 DAJSVP010000000
VERSION     DAJSVP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.144"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(243..392)
                     /locus_tag="ON449_002593"
     CDS             complement(243..392)
                     /locus_tag="ON449_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935111.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ON449_002594"
     CDS             854..1066
                     /locus_tag="ON449_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935112.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ON449_002595"
     CDS             complement(1344..3143)
                     /locus_tag="ON449_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3935113.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ON449_002596"
     CDS             complement(3267..3863)
                     /locus_tag="ON449_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3935114.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1447 a          711 c          687 g         1177 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatcc cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattatagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 ta
//
LOCUS       DAJSVP010000140         3916 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSVP010000140 DAJSVP010000000
VERSION     DAJSVP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.145"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(25..1530)
                     /locus_tag="ON449_002597"
     CDS             complement(25..1530)
                     /locus_tag="ON449_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3935115.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="ON449_002598"
     CDS             complement(1527..2213)
                     /locus_tag="ON449_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3935116.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="ON449_002599"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="ON449_002599"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3935117.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DAJSVP010000141         3801 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSVP010000141 DAJSVP010000000
VERSION     DAJSVP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.147"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="ON449_002600"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="ON449_002600"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3935118.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="ON449_002601"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="ON449_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3935119.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="ON449_002602"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="ON449_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3935120.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="ON449_002603"
     CDS             2855..3730
                     /locus_tag="ON449_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3935121.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         1301 a          661 c          853 g          986 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta a
//
LOCUS       DAJSVP010000142         3780 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSVP010000142 DAJSVP010000000
VERSION     DAJSVP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.148"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            95..592
                     /gene="dfrG"
                     /locus_tag="ON449_002604"
     CDS             95..592
                     /gene="dfrG"
                     /locus_tag="ON449_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3935122.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(664..2616)
                     /locus_tag="ON449_002605"
     CDS             complement(664..2616)
                     /locus_tag="ON449_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3935123.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2623..2859)
                     /locus_tag="ON449_002606"
     CDS             complement(2623..2859)
                     /locus_tag="ON449_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935124.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3097..3780
                     /locus_tag="ON449_002607"
                     /pseudo
     CDS             3097..3780
                     /locus_tag="ON449_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1144 a          667 c          629 g         1340 t
ORIGIN      
        1 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
       61 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      121 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      181 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      241 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      301 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      361 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      421 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      481 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      541 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      601 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      661 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      721 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      781 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
      841 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
      901 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
      961 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1021 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1081 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1141 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1201 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1261 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1321 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1381 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1441 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1501 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1561 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1621 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1681 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1741 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1801 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     1861 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     1921 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     1981 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2041 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2101 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2161 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2221 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2281 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2341 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2401 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2461 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2521 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2581 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2641 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2701 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2761 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     2821 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     2881 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     2941 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3001 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3061 attttcactt tttgtactcc aaaactgaac tacttatttt tatttagttg tctctatatt
     3121 ttctctgctt atcggacagt agacatctac tatcgttccg caattgccga agctatcgca
     3181 ataacacttg ttatccctgt attattttat gcttatcaaa tcatctctgg aaaagaagaa
     3241 aagtatccgt ctgtaaagct agcattgagt atgagtttgc tagtgtacag ccatgtacta
     3301 tcaacattaa tgagtacagc attaattatc atttttattt ttattcgttt ggtttctaaa
     3361 ggtttcaaga atgcagattt tattgctata tttaaaaaat tatttagtgc ggcaggaatg
     3421 acattggttc tgacgagtgc tttttggtat ccgatgtttg aacaaatgct ctaccaaaaa
     3481 atcaacaaac ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt cgatagtctg
     3541 actgaagcta tgaacaacga cttaactact tactcaatgg gactagttgg tctgttatct
     3601 ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca tagaaaaaaa gatttattat
     3661 ggtacgtgtt tgacttggct agctacaact agtttggtgc catggtatct tctgcaaaat
     3721 acgccagcca aattgttaca atttccttgg cggatcttaa gcttgcaaat aatattctct
//
LOCUS       DAJSVP010000143         3732 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSVP010000143 DAJSVP010000000
VERSION     DAJSVP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.149"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ON449_002608"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ON449_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3935125.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="ON449_002609"
     CDS             1228..2331
                     /locus_tag="ON449_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3935126.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="ON449_002610"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="ON449_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3935127.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1131 a          748 c          634 g         1219 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aa
//
LOCUS       DAJSVP010000144         3653 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSVP010000144 DAJSVP010000000
VERSION     DAJSVP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.150"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(168..1235)
                     /locus_tag="ON449_002611"
     CDS             complement(168..1235)
                     /locus_tag="ON449_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3935128.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1255..1734)
                     /locus_tag="ON449_002612"
     CDS             complement(1255..1734)
                     /locus_tag="ON449_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HCR3935129.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1751..2212)
                     /locus_tag="ON449_002613"
     CDS             complement(1751..2212)
                     /locus_tag="ON449_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3935130.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2226..3476)
                     /locus_tag="ON449_002614"
     CDS             complement(2226..3476)
                     /locus_tag="ON449_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCR3935131.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1125 a          769 c          488 g         1271 t
ORIGIN      
        1 gtaccgtgct tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt
       61 cttacacgga taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac
      121 ttaataagtt cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc
      181 cctttttgat acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt
      241 gccaaatcct tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt
      301 attgcagatg tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca
      361 cgtactgctg aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc
      421 gcctctagaa tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat
      481 cttctatagc actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat
      541 tgcaaattat attcttctat caattctttg agcgatattt cttcatcacc tgctccagca
      601 atctcaaaaa taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc
      661 aaatcgtgta caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt
      721 gaattccact ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta
      781 caatttggct caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa
      841 tcagactctc ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac
      901 cattttttaa attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac
      961 cacttattca gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata
     1021 atattaggtt tactcaataa agagatacct ataattttag caaatgcata cataaaatat
     1081 aaagatttaa atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc
     1141 tttttttccc aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc
     1201 gagctgtcat taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat
     1261 tttttctatt aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt
     1321 aaacatccga actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg
     1381 atagataata ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg
     1441 ctcaccatac ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc
     1501 aattatagca cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt
     1561 aaactcatcc ctatttagaa atgctttata ggaataattt agtggtttat aatcactata
     1621 accactctgt gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc
     1681 ttttaacaat ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt
     1741 ctcctattat ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag
     1801 tttcccactg aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag
     1861 ggctggttac ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta
     1921 ttaagcagat agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct
     1981 ttatatatat aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt
     2041 cttttctatt tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt
     2101 ctgtctgttc tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta
     2161 actgttcata atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct
     2221 ctctatcagt cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag
     2281 caggtatcat atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg
     2341 ttgataacct tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc
     2401 acagtcgcac ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa
     2461 ttatcactgt tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg
     2521 ttgacatcat actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga
     2581 tctgcgatga aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca
     2641 ggaacatctg catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca
     2701 tatccaaagg atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg
     2761 attatctgct gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag
     2821 ctgactcgca gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat
     2881 cccataaaaa gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa
     2941 acatgagcag catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat
     3001 ggatacagat tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg
     3061 ttcttactcc ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg
     3121 atgaccatga tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca
     3181 gaagtatcga aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa
     3241 tctgcggatt tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct
     3301 gtaagatact tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca
     3361 atcttttcag ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg
     3421 atactcaaac caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata
     3481 tctcctcttt cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa
     3541 aataacacag cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca
     3601 tttataaacc acacatctaa ttttatctat tctccacaag acatgtagag tat
//
LOCUS       DAJSVP010000145         3624 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSVP010000145 DAJSVP010000000
VERSION     DAJSVP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.151"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            746..829
                     /locus_tag="ON449_002615"
     CDS             746..829
                     /locus_tag="ON449_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3935132.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            954..1691
                     /gene="erm(B)"
                     /locus_tag="ON449_002616"
     CDS             954..1691
                     /gene="erm(B)"
                     /locus_tag="ON449_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3935133.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1954..2070)
                     /locus_tag="ON449_002617"
     CDS             complement(1954..2070)
                     /locus_tag="ON449_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HCR3935134.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            2333..2845
                     /locus_tag="ON449_002618"
                     /pseudo
     CDS             2333..2845
                     /locus_tag="ON449_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2894..3574
                     /locus_tag="ON449_002619"
     CDS             2894..3574
                     /locus_tag="ON449_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3935135.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1197 a          597 c          687 g         1143 t
ORIGIN      
        1 ttgacaacca tttttgttag gcgtttgagg aagtgaagct gttttcctca tgttttaaaa
       61 gctagaaata aattacagtg tacgctcctt tcttttttcg ttttgttggt tcattttcgc
      121 cagtgtgttc gtgtcgtttg ctgtgacgag tacgcaacat agacacaaag aaaacacaag
      181 ggcgagcttg ctcgtttata tacccttgtg ttttcgactg gatatgttaa ggtaacaaac
      241 agtaaatgac acgttacaga ctgcaaaatg taccaactga aaaaagaaag aataccgttg
      301 tgtgttttat ttctcgatca gcttttaaaa catggggtcc cgagcgccta cgaggaattt
      361 gtatcgataa gaaatagatt taaaaatttc gctgttattt tgtacattta acttgacggt
      421 gacatctctc tattgtgagt tattagtggt acagttttca accgttttaa ttataaaaaa
      481 gtggtgcatt tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct
      541 atgggtttaa catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct
      601 tgcgtatggt taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
      661 tgatagtgca ttaaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      721 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgttatg tagataaaac
      781 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
      841 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
      901 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagtgat tacatgaaca
      961 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     1021 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     1081 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     1141 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     1201 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     1261 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     1321 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     1381 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     1441 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     1501 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     1561 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     1621 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     1681 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     1741 aatgtagata aattattagg tatagggcac ctctaataac taccaattaa ttcacctcaa
     1801 aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
     1861 cgtaggcgct cgggacccca acatgaggaa atgtttttat aaaaaaagag tagttaccgt
     1921 tttttggtaa ctactctttt ccttttttaa ggtttaaata cctggaagtt taggtgtttt
     1981 gggtattggt ggtgtaggtg gctgcaagct ttccaatttt tgttgaattg ctttaaattc
     2041 aggggtttct tggtgttgat tttgaaccat tttttgctct attctctcca aagtcggttg
     2101 aatttctttc cctgatactt tacgatttaa ttctctttct aaggtctctt tcggactaat
     2161 ggaaggcgtt tctaagcttg aatagagttt cactccttct ctattgtaaa gccttatatc
     2221 gctaaataag cccgttttat gaagggtttc taaattggtc ggtaagtttt tgacaacaat
     2281 atcatgcgct tgttttggtg gtgccctagc tgtcattgga tcatctgcat acaataagat
     2341 ccatgaaagg ccatctgctg ccaatgagcg ccttgagatt ggtaattggg aagctgatac
     2401 tgtagccggt aaaacaggta gggctcgtat tgtaactcta gtcgatcgta gatctcgttt
     2461 ctccctactt ggtaaggttg aaaagaaaat ctctaaaaat gtggttgatt gtatgatcaa
     2521 tctactaaga aatgtaggag aagataaatg taaaaccgtt acacccgata gaggaaaaga
     2581 attctcccaa catagtcgaa taacgctaga attaaatggc actaaagtgt attttccaga
     2641 tccacatgct ccatggcaac gtggtacaaa cgaaaatact aatggtttat tacgagaata
     2701 ttcacccaaa ggtgaagata tttcaggtat tttcgatagt caaattcaag aatgggcgaa
     2761 taaattaaac acgcgaccac ggaaatgtct aaattggaaa accccttatg aagtattttt
     2821 ggttctgttg caaagttccc cataactcct gatttattgt aaaatgtaag aaaacgagag
     2881 tgagggtaga cagatgaatc atttcaaagg gaaacaattt caaaaagatg tgattattgt
     2941 cgctgttggt tactacctgc gttacaatct gagctatcgt gaagttcagg aattactgta
     3001 tgatcgcgga ataaatgttt gtcacactac gatttatcgt tgggttcaag agtacagtaa
     3061 aatcctctat catctttgga agaagaaaaa tagacagtcc ttctactcgt ggaaaatgga
     3121 cgagacctat atcaaaataa aaggccattg gcattatctc tatcgtgcga ttgattctga
     3181 tggattaact ttagacattt ggttacgaaa gaaacgggat actcaggcgg cttatgcttt
     3241 cttaaaacgg ctccataaac aatttggaca acctagagtg attgtaaccg ataaagcacc
     3301 atcgattagc tctgcattca gaaagctaca gattaacggt ttatatacca agacagaaca
     3361 tcggacagtt aagtatctca ataatttaat tgagcaggac catcgtccaa ttaagcgccg
     3421 taataaattt tatcgaagtt tacgaactgc ctcaaccacg attaagggca tggagactat
     3481 tcgaggaata tacaaaaaga accgaagaaa cggaacgctc ttcggatttt cggtgtcgac
     3541 tgaaattaaa gttctgatgg ggatcccagc atagtcaaaa tattaaacta ggacttctat
     3601 actcttttgg ggaactttgc aaca
//
LOCUS       DAJSVP010000146         3596 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSVP010000146 DAJSVP010000000
VERSION     DAJSVP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.152"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            41..1030
                     /gene="galE"
                     /locus_tag="ON449_002620"
     CDS             41..1030
                     /gene="galE"
                     /locus_tag="ON449_002620"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3935136.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1042..2526
                     /gene="galT"
                     /locus_tag="ON449_002621"
     CDS             1042..2526
                     /gene="galT"
                     /locus_tag="ON449_002621"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3935137.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2550..3530
                     /locus_tag="ON449_002622"
     CDS             2550..3530
                     /locus_tag="ON449_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3935138.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1215 a          654 c          799 g          928 t
ORIGIN      
        1 atcaacaaac acaattttac taatcaaagg agtgtttttt atgacaattt tagtactagg
       61 cggagccggt tatatcggtt cgcacgctgt agaccagcta gttcaaaagg ggtatcaagt
      121 agctgtggta gataatttgc tcacaggtca caaacaagca gtacatccag atgctcattt
      181 ttatgaagga gacatccggg ataaagaatt tctacgttcc gtatttgaaa aggaaccgat
      241 tgaaggagta atccattttg ctgcaagctc tttagtagga gaatctgtcg aaaaaccatt
      301 gatgtatttc aacaacaacg tatacggtat gcaaatctta cttgaagtca tgcacgagtt
      361 caacgtgaat aaaatcgtat tctcttctac tgcagcaact tatggagagc cgaaagagtc
      421 tccaatcaca gaagatacac cagctaatcc taaaaatccg tatggtgaaa gtaaattgat
      481 gatggaaaaa atgatgaagt ggtgtgacca agcatatggc atgcgctatg tcgctttgcg
      541 ctacttcaat gtagccggag caaaagcaga tgcatctatc ggagaagatc atacaccaga
      601 aacgcatctt gtcccaatca ttttacaagt agctcttggt caaagaaaag ccttggctgt
      661 ttatggagac gattatgata caccagatgg aacatgtatc agagattatg ttcaagtaga
      721 agatttgatt gctgcacata ttttagcttt ggaatacttg aaagaaggaa atgaaagcaa
      781 cttcttcaat ttaggcagca gcaaaggata ctcagtcaag gaaatgctag aagcagctag
      841 agaagtgaca ggaaaagaaa ttccagcaga aatagcacca agaagagctg gagatcctag
      901 ccgattagtc gcttcaagtg aaaaagcgag agaggttctg ggttggaaac ctgaatacac
      961 agatatcaaa gcaattatca aaacagcatg ggattggcat gtcagccatc caaatggtta
     1021 tgaagaatag gggaatgaga tatgtcaatc agtcaaacga tttgtgattt tgccacactt
     1081 gcgatcgaag ctggcggatg gatggaaatg gaccgcttat atttacagaa tcgtctttta
     1141 gggatggtag gagaagatgc tctagaaagc attgaagtaa gaccagttaa ggaagaatct
     1201 gtagacttat tggatcagct tgtcgctaag gcaagagaaa atggtgttct ttcagaattg
     1261 ccagcagaac aagagatttt tgaagcgcaa ttaatggatt ttctcacacc gccaccttca
     1321 gtcgtcaatg cattctttgc acagcactat tcgaaaagcc cagaggaagc aactgagtat
     1381 ttctttaatt taagtaagaa aaatgactac atcaaaacaa gagccatcgc aaaaaacatc
     1441 aaatttccgg cagagtcgga gtatggtgaa ctggaaatca caatcaattt atctaagcca
     1501 gaaaaagatc caaaacaaat cgcagcagaa cgcgaggcgg taaaagtaga ctatcctacc
     1561 tgcatgcttt gcatggaaaa cgaaggatat agaggtcgct tgaactatcc agcccgcacc
     1621 aatcaccgga ttattcgaat gaatctagac ggtgaaagct ggggattcca atattctcct
     1681 tatgcttatt acaatgaaca ttcgatcatt ttatcagaac aacaccgccc gatgaaaatc
     1741 acaaaggaaa catttgctag actgcttcgg attactgaag tcttgcctca ttactttgtt
     1801 ggttcgaatg ctgacttgcc gattgttgga ggatcgattc tttctcatga tcattatcag
     1861 gcaggacgtc atgaatttcc tatggcaaaa gcaccgatag aaaaacttat caaactagca
     1921 gattatccgc aagtcatcgc aggagtcgtg aaatggccga tgtctgtgat ccgtttgcaa
     1981 tcaacagaga aaaaattgtt gattgaagca gctaactcag tattggaaaa gtggaaagca
     2041 tattcggatg aaacagtatc tatcagagct ttttcagcag atggtacacc acaccatacg
     2101 attacgccaa ttgcacgaag aagggacaat tactttgagt tggaccttgt ccttcgagac
     2161 aacaatgttt cagaagaaca tcctgatggg atttttcatc cacataaaga tgtccaacat
     2221 atcaaaaagg agaacattgg gttgatcgaa gtaatggggc ttgctgtttt accacctcgt
     2281 ttgaaaccag aactagtaga agtagagcat ttcttattag gagaagaaaa ccagatggct
     2341 gaatatcatc gtccgtgggc agaacaatta aaaaaagaac atcctgattt gacgaaagat
     2401 acggtcaccg atgtcgtaca aaaatctgta ggtcaggttt ttgcacgtgt actggaagac
     2461 gctggagtat tcaagcgaga tgaaaaagga caggcagcgt ttgaacgatt tactgcaatt
     2521 ttataaacgt aaaaaaggag gagagacaaa tggcaacaat taaagacatt gcgcagttgg
     2581 caggagtttc tcctgctact gtttcaagag ttctcaacta tgatcctgat ctttccgttg
     2641 gcttagccac aaagcaaaaa atttttgaag cagcagaacg cttaaattat acgaaacatc
     2701 gtaaaaatgc agcaaatact aagcaaacgc ttcgcctgat ccagtggtac gacgatacgg
     2761 aagagcttga agatctttat tatttatcta ttcgtctagg aatcgaaaaa aaggcagaag
     2821 aagcaaacgt tactttattg aaagaaacgt gggattccat tagtgatctt ccgacagatg
     2881 gaacgatcgc actaggaaaa ttcgacgaag ctcagttatt agaaatgaaa ggatcccaaa
     2941 atgccttgct tttggtagat tctgacggca cttctcaagg aatcgactct ctagttgtcg
     3001 actttaagtc aagtgttcaa aaagtcattg aacattttgt ctcccataaa gcgcaacata
     3061 tcgctatgct tgctggaaca gaatatacga agaaaaacca tcagcggcta aaagatccca
     3121 gaattatggc atttcaacaa gttttggcag aaaaaggcta cgacgaaggt ccaattatcg
     3181 aagcagattt ttctgtcgag tcaggatatc aagcggtgaa gcagtattta aaaacgaatc
     3241 cgcatgttcc tgatgcgctt tttgcagcta atgatgcatt agcgattggc gcactgaaag
     3301 cgattcaaga aaaaggttta gtggtaccag aagatatttc tgtgatgggt ttcaatgatg
     3361 tcagtgtagc gaaatatgta acgccctctc tttctactgt aaaagtttat accgaatgga
     3421 tgggagagct agcagtggag acgattcttc aattaagtaa ttccaaagca ccggtttcaa
     3481 gaaagattat tgtggaaaca gagttgatta ttcgagaatc aacgaaataa aagatggaaa
     3541 agctgtgggg aaatgaagtg ataccgcagc ttttttgtgt ttcaggaagg ggcaaa
//
LOCUS       DAJSVP010000147         3480 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSVP010000147 DAJSVP010000000
VERSION     DAJSVP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.153"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2232)
                     /locus_tag="ON449_002623"
     CDS             complement(<1..2232)
                     /locus_tag="ON449_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935139.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ON449_002624"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ON449_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          977 a          769 c          549 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
//
LOCUS       DAJSVP010000148         3308 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSVP010000148 DAJSVP010000000
VERSION     DAJSVP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.154"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(451..1761)
                     /locus_tag="ON449_002625"
     CDS             complement(451..1761)
                     /locus_tag="ON449_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3935140.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ON449_002626"
     CDS             complement(1773..3191)
                     /locus_tag="ON449_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3935141.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1018 a          673 c          512 g         1105 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaa
//
LOCUS       DAJSVP010000149         3299 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSVP010000149 DAJSVP010000000
VERSION     DAJSVP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.155"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            49..1089
                     /locus_tag="ON449_002627"
     CDS             49..1089
                     /locus_tag="ON449_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3935142.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            1913..2245
                     /locus_tag="ON449_002628"
     CDS             1913..2245
                     /locus_tag="ON449_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3935143.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2864..3205
                     /locus_tag="ON449_002629"
     CDS             2864..3205
                     /locus_tag="ON449_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3935144.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1234 a          451 c          632 g          982 t
ORIGIN      
        1 gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt
       61 aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt
      121 attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta
      181 aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat
      241 tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata
      301 gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac
      361 aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa
      421 aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca
      481 gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct
      541 caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc
      601 ataccagaac ttaataataa tatattagac actaatagac acaatataga cactgaaaaa
      661 gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc
      721 attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac
      781 agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa
      841 agtggtattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa
      901 ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc
      961 cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa
     1021 gtatcaagtg aaaaacgttt gaattatgaa agctccccaa gcactattac gaaagactgg
     1081 ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat taaaattaaa
     1141 aattttttat tattctaatt atattacgaa tattacaaaa taatagctat tttaatagaa
     1201 tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt
     1261 aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt
     1321 ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt
     1381 gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta
     1441 attgtctgct atgcttgtga tgaattgatt ttcgttctgc acttttggat gataaatttg
     1501 gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg
     1561 tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg
     1621 gtattacccg caatggattt agtataacag atattcaaaa gtgataccac tttttttggc
     1681 tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca caatgttata
     1741 aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt
     1801 tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa
     1861 ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata
     1921 tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga
     1981 gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata
     2041 tataatccag aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca
     2101 aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta
     2161 tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa
     2221 agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt
     2281 tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa
     2341 ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat
     2401 tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga
     2461 atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag
     2521 aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca
     2581 tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc
     2641 tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc
     2701 tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt
     2761 taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattgatat
     2821 aaaacataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc
     2881 gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg
     2941 atttgtgacg tttagaagag gcggaaattg taaaggatta gatgttttag cattagacga
     3001 ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga
     3061 agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt
     3121 atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga
     3181 atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta
     3241 gaaggagtca cgatgattaa taaaaaggta gtattacctt aggcattgag taaggaatt
//
LOCUS       DAJSVP010000150         3299 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSVP010000150 DAJSVP010000000
VERSION     DAJSVP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.156"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(551..2164)
                     /locus_tag="ON449_002630"
     CDS             complement(551..2164)
                     /locus_tag="ON449_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3935145.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="ON449_002631"
     CDS             complement(2305..3213)
                     /locus_tag="ON449_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3935146.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1075 a          564 c          577 g         1083 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaa
//
LOCUS       DAJSVP010000151         3298 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSVP010000151 DAJSVP010000000
VERSION     DAJSVP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.157"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..387
                     /locus_tag="ON449_002632"
     CDS             <1..387
                     /locus_tag="ON449_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3935147.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            491..715
                     /locus_tag="ON449_002633"
     CDS             491..715
                     /locus_tag="ON449_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935148.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            832..1329
                     /locus_tag="ON449_002634"
     CDS             832..1329
                     /locus_tag="ON449_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3935149.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            1304..1810
                     /locus_tag="ON449_002635"
     CDS             1304..1810
                     /locus_tag="ON449_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3935150.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1794..>3298
                     /locus_tag="ON449_002636"
     CDS             1794..>3298
                     /locus_tag="ON449_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3935151.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT         1040 a          598 c          691 g          969 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatcaggaa cttacctatc catatataac tggtttctat caaacgctca
      541 atcactggtg cttttagcaa tcgttgtgat tggcttgtat cttggcttca agcgtgagtt
      601 tagcaaactg attggctttt taattattgc gattattgcg gttggcttag tcttcaacgc
      661 tgctggagta aaagacattt tactagagct attcaatcgc attattggtg cttaaataaa
      721 accgttcttt tgtggaatat aagtggtttt cttatgttcc gcaaaggaat ggtacaccaa
      781 acgaagtgcg gtagggattt ttgaatctct acaaagaaag gacgtgaata tatggacgat
      841 atgcaagtct atattgcgaa tttaggcaaa tacaatgagg gcgaattggt cggtgcgtgg
      901 tttacctttc ccattgactt tgaggaagtc aaagagaaaa tcggcttgaa tgatgaatat
      961 gaggaatacg ccattcatga ctacgagtta ccctttacgg ttgacgaata cacttccatt
     1021 ggcgaactca atcgactatg ggaaatggta tcggaattac ccgaagaatt acaatcggag
     1081 ctatctgctc tgctcactca tttttcaagc attgaagaac taagcgaaca tcaagaggat
     1141 attatcattc attccgattg tgatgatatg tatgacgtgg cacgctacta cattgaagaa
     1201 acgggtgctt taggcgaagt accagctagt cttcaaaact atattgatta tcaagcctat
     1261 ggtcgggatt tagacctttc aggaacgttt atctcaacca atcatgggat ttttgaaatc
     1321 gtctattaaa tctgtcggta cattactact ggcagatttt ctattttacg gggtggctca
     1381 atcagctacc cctatttttt atgaaaggat tgattacatg aagaaaatac gaagctatac
     1441 cagtatctgg tctgtggaaa aggtactgta ttctatcaat gattttagac ttccgtttcc
     1501 cataaccttt acgcaaatga catggtttgt cgtgtcactc tttgcagtga tgatacttgg
     1561 caacttgccc cctctttcca tgatagaggg agcatttctc aaatactttg ggattcctgt
     1621 ggctttcaca tggtttatgt ctacaaaaac ttttgatggt aaaaagcctt atggattttt
     1681 gaagtctgtc attgcttatg cactgcgacc aaagctgacc tatgcaggaa aaaaagtaac
     1741 gcttggcaga aaccagccac aagaagccat tacagcagtt aggagtgaat tttatggcat
     1801 atccaattaa atacattgaa aacaatctcg tctggaataa agacggggaa tgttatgctt
     1861 actatgagct tgttccttac aattactcat ttctaagtcc agaacagaaa atacaagtgc
     1921 atgattcttt cagacagctt atcgcacaaa atcgtgatgg caaaattcat gctttacaaa
     1981 tcagtacaga atccagcata cgttctgcac aagagcgttc caaaaatgaa gtcactggca
     2041 agctcaaagc ggttgcctat gacaaaatcg accaacagac agacgcttta atatccatga
     2101 ttggcgaaaa tcaagtgaac taccgtttct ttatcggctt taagttgctt ctcaacgatc
     2161 aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa tgctttgtct gattttgtct
     2221 atgatgtgaa ccataagctg atgggcgatt ttgttagtat gagtaatgat gaaatcctgc
     2281 gttttcagaa gatggaaaag ctcttagaaa ataaaatctc tcgtcgtttc aaaatccgca
     2341 ggttagataa ggacgacttc ggctatctga ttgaacacct ttacggacag acaggcactg
     2401 cctatgaaga gtatgagtac catctatcaa agaaaaagct ggataatgaa acgctgatta
     2461 aatactatga cttgattaag cctactcgct gtttggtgga agaaaaacag cgatatttga
     2521 aaatccagca ggaagatgaa accgtctatg tagcttactt taccattaac agcattgtcg
     2581 gagaactgga cttcccgtcc tctgaaatct tctactacca gcaacagcaa tttacattcc
     2641 cgattgatac gtcaatgaat gtggaaattg tagcgaatcg taaagcccta tctactgtcc
     2701 gcaataaaaa gaaagaactg aaagacttgg ataaccacgc ttggcaaagt gataatgaaa
     2761 ccagctccaa tgtggcggaa gctctggaaa gtgtgaatga gctggaaacc aatttagacc
     2821 aaagcaagga atctatgtac aagctgtctt atgtggtaag ggtatcagca aatgatcttg
     2881 acgaactcaa acgtcgttgt aatgaagtga aagattttta tgacgattta agcgtaaaac
     2941 tggtacgacc atttggggat atgctcggct tacatgaaga atttttacct gccagcaagc
     3001 gttatatgaa tgattatatt caatacgtga cctctgattt cctcgctggt ttaggttttg
     3061 gtgctactca aatgctgggg gaaaatgagg ggatttatgt tggctacagc ttagatactg
     3121 gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg ggttaagggt tcagtaacca
     3181 atgcgttagc gtcggctttt gttggttcgc tgggtggtgg taaatccttt gcgaataacc
     3241 ttatcgtcta ttatgcggtg ctttatgggg cacaagcagt gattgtagac ccaaaagc
//
LOCUS       DAJSVP010000152         3247 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSVP010000152 DAJSVP010000000
VERSION     DAJSVP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.158"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            101..478
                     /locus_tag="ON449_002637"
     CDS             101..478
                     /locus_tag="ON449_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3935152.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            466..804
                     /locus_tag="ON449_002638"
     CDS             466..804
                     /locus_tag="ON449_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3935153.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            938..1381
                     /locus_tag="ON449_002639"
     CDS             938..1381
                     /locus_tag="ON449_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3935154.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1643..2458
                     /locus_tag="ON449_002640"
     CDS             1643..2458
                     /locus_tag="ON449_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3935155.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2468..3130
                     /locus_tag="ON449_002641"
     CDS             2468..3130
                     /locus_tag="ON449_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3935156.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT         1036 a          485 c          657 g         1069 t
ORIGIN      
        1 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
       61 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
      121 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
      181 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
      241 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
      301 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
      361 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
      421 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
      481 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
      541 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
      601 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
      661 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
      721 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
      781 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
      841 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
      901 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
      961 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
     1021 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
     1081 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
     1141 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
     1201 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
     1261 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
     1321 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
     1381 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
     1441 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
     1501 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
     1561 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
     1621 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
     1681 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
     1741 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
     1801 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
     1861 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
     1921 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
     1981 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
     2041 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
     2101 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
     2161 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
     2221 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
     2281 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
     2341 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
     2401 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
     2461 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
     2521 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
     2581 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
     2641 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
     2701 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
     2761 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
     2821 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
     2881 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
     2941 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
     3001 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
     3061 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
     3121 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
     3181 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
     3241 acttttg
//
LOCUS       DAJSVP010000153         3243 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSVP010000153 DAJSVP010000000
VERSION     DAJSVP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.159"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1018
                     /locus_tag="ON449_002642"
     CDS             <1..1018
                     /locus_tag="ON449_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3935157.1"
                     /translation="KLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNEL
                     TDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLV
                     TQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVA
                     FCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDIN
                     YIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCH
                     SLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKH
                     DILI"
     gene            1074..1292
                     /locus_tag="ON449_002643"
     CDS             1074..1292
                     /locus_tag="ON449_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935158.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1363..2178
                     /locus_tag="ON449_002644"
     CDS             1363..2178
                     /locus_tag="ON449_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935159.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2242..2427
                     /locus_tag="ON449_002645"
                     /pseudo
     CDS             2242..2427
                     /locus_tag="ON449_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            3001..>3243
                     /locus_tag="ON449_002646"
     CDS             3001..>3243
                     /locus_tag="ON449_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935160.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIP"
BASE COUNT         1240 a          366 c          573 g         1064 t
ORIGIN      
        1 aaaacttcag atgggtgaaa ttgttagagg tcctagtcaa atatctattg atgttacata
       61 taaatgtaat tttagatgta aacattgtta caatgcgagt ggtggagaca attgttttat
      121 ggataatgag ttgactgatg atgaactgtt agaacttaca aaagatatat gtaatttaaa
      181 gccatataat atttgtttct gtggaggaga aactttactg agaaaaaaaa tagttttatc
      241 ttgtgccact ttattaaaag aggcaggtat tcctaatatt gctattgtta ctaatggatc
      301 attagtaact caggatgttg caaaagaatt attcaaagca ggtattaatc gatttcaagt
      361 aagtttagat ggaaaaaatg ctagtagcca tgaagcactt agattatatc caaacagttt
      421 tgaaaaggca attaatgcta tcaaattatt tagagaattg tccgaagaag tggatgttgc
      481 tttttgtcct actagtttta attacaatca attacgagaa gtatatcaac tagtaaatac
      541 tcttaaagtg aagtctttaa gaattcaacc atttatgctt agtggtaggg gaatgcaaaa
      601 tagggaaata gtgccaactg tttctcaata tagaaaacta gtttctgata ttaactatat
      661 taaaagaaat gaagattata ccaagataga ttggggagat ccaattgatc atatcatccg
      721 acacagaact ttattgaaag atttttcgaa ttttgtttct attaggtcag acggaggatt
      781 aatggcatca ccttacttgc caattcaagt tggaaatatt aaatgtcaca gtctttctaa
      841 atattggcga gcaggacttt ataaagtttt ttctttagat ttagttcaag atatagctac
      901 tcaagtttct tcaatacaag atatgagtaa agaattaaaa aaaggacata ttaaatggct
      961 taatgaagat atagaaattg atattctcga tgaatacaaa catgatattt taatttaggt
     1021 ttatcattgg aaagtttaga taaacaaagt acttttagaa aggagggaaa aaaatgattt
     1081 ctgaacctca aatgaataat atgtatgaaa gcccaactat aatgggagct ggtgaagctg
     1141 cgccaagagc ttgttcagca attatggtgt gtgtgttatt agcatctgca ggagtaggta
     1201 tccaagcagg agcggtgtat acttatgctg tagttgctac agtagctgta gctgcagccg
     1261 cagtttggaa aacagtagga ttagttggtt aggaattaat aggagactaa ctgtctccta
     1321 ttgattccaa tgattaagct aaggaggaat tttaagaaaa taatgaaata caataattta
     1381 aaaccgaaat tacttaatga gaacatagaa tatataagag aagaaaatta tggatattta
     1441 accatagttc ccaaaaatgg aactgaattg aaagataacg ttttcaatcc aacagtaaga
     1501 tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa tggtttaaaa
     1561 aagaagtttc ctagtttaga atatgaaaaa ttctatcaag atgttgtagg aactattaaa
     1621 tttttcaaac actataatat tattgatata ggagattata gtatggaaga aaagaaaata
     1681 tataaaattg aacaaaattt agaattagaa ttactaaatg aatcacatta tgaagaaatt
     1741 tataatttta tcacagatca tcctgaaagt atgtattttc atgaagatat ttatcatcaa
     1801 ggaaatagta aagaattaga agttgtcctt agatataatt tattttcgtt tacgagtgaa
     1861 ttttactttt taagacataa agagagaatt gtaggaatgg tttctataat tgttaatcca
     1921 tttcaaaact tatcctacag tatagtaaat tacctcatat gtgagcctgc atatactacg
     1981 actattttga caacagtatt agaaaatatt aacgaagttt caattttaga agtaaagaag
     2041 ctagtgattc atttgctaaa ttcaacagaa gatgaatcac taatccccta tttaaaagat
     2101 tcacattttt ttaaagaagc ggtactatct aaagaatata gagagcatga tgttttgaga
     2161 tacgcttaca tttattaaat aaaatttctg atagtatttt aataattcgg atcatatcat
     2221 aagatatagg agggaaaaga agtgatttta ttgattttgt ctctcgtatt actagttgta
     2281 tctaatctaa tgaaaaataa tcctcctaca aaaattaatt ctttttatgg ctttagaact
     2341 aaaaagtcaa tgaaaaatga aaaaagttgg catttagcac aaaaattgtt gggggatttt
     2401 tcaaaaaaaa aagttatccc agtatttttg atgggtattg tttttttcag tatagagatt
     2461 tatagtttag ctattttaaa aaatgaaaat tttttcgtta gtgtattaat gggtgaaagt
     2521 ttaatcctaa tactgatttt gtgtttaatt gttcatgaag tgaataaaca attgtgaatt
     2581 aaaaaaataa aaacctagta taatcacttt aataaattgt tggtagaagg tgatcaaaat
     2641 gggagtaaac gaaatgatga aagtcagatt gagtttttaa atgatacctt cctcagaact
     2701 tcaaaacgaa ttcaaagatt cgttgacaaa tcttgagcaa gatcatttaa aaaaatattt
     2761 tctcttcatt tgacgaaaaa tgtttagaaa tcttatataa gtgaagataa agtaattggt
     2821 gtaattaatt gtagagaata gcctttgagc aattgaagcg atacaattat ttagaaaatg
     2881 aacttcctcc agcatactac atgattcctt acgggacgat gttaaagaag aaagcaagaa
     2941 attttagaag tattagtaga taaactaatg ggcttttctc aatttaaggg agtagcccaa
     3001 atgtttatca aatatggtgc agatacagta gcaaccatgt ttacagaaga atatcaagta
     3061 gactattatg aagtaataga taaatgtgta cattttgttt atgaaaaagt gtggaatttt
     3121 atgaattttg cgataagtga agaaatctta cgagtacaag aaaacagaac aggaatattt
     3181 accagagaca gaaagcttaa tttgattgcg cattatatac ggaatcgctg gtcatcaata
     3241 cca
//
LOCUS       DAJSVP010000154         3219 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSVP010000154 DAJSVP010000000
VERSION     DAJSVP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.160"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(271..639)
                     /locus_tag="ON449_002647"
     CDS             complement(271..639)
                     /locus_tag="ON449_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3935161.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            789..1271
                     /locus_tag="ON449_002648"
     CDS             789..1271
                     /locus_tag="ON449_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3935162.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1308..2318
                     /locus_tag="ON449_002649"
     CDS             1308..2318
                     /locus_tag="ON449_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3935163.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2433..3146)
                     /locus_tag="ON449_002650"
     CDS             complement(2433..3146)
                     /locus_tag="ON449_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935164.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1087 a          519 c          624 g          989 t
ORIGIN      
        1 ttatgaaaga tcactgatat tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa
       61 attgctgaaa aaataatttt actaagtgga aaattcaaag ccattcaatc agtgattttt
      121 ttcagcacaa gaaagactcc ttataaaaat agcagatact ttgtcaatta agtgtttaac
      181 cagggatgat aaaaaggata ggattgaaca aaacctgtat aagctgatga atagtttcct
      241 cgccagtaaa aggtttattc gtataaagct ttatcgccaa aatttatcat gacctttttt
      301 atcttcaata ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca
      361 aggaactttg atgacgttat ccgtatatac gtagcctgct tcttggatat ttttttcaag
      421 ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc
      481 aataataaag gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag
      541 ctggggatac gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt
      601 ttctttgatc tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt
      661 ggtttgattt attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag
      721 taatgttgta accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag
      781 acataaaaat gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt
      841 atcatcatcg agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat
      901 ttacaggtac agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat
      961 atctgacttt tactacgaaa caaacggatt tcaaagcatt agggaagatc gtcggtaaaa
     1021 ggctttctat tgtttcatca gaacgcatgg aagaacaaac gggacaaaag cagggcgctg
     1081 tttctccgtt tggttatgaa aaacagattc cgattattat tgatgaagag ctcttatcaa
     1141 atgaaaaact tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg
     1201 acttaaacaa aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa
     1261 aaaacaatta accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc
     1321 cattattaga aagaaatgca acgattgggg ttactgcacc atcctcaggc gtatcagagg
     1381 aacaccattc gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag
     1441 ttactggaaa gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag
     1501 cagatgaatt caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgccttggg
     1561 gaggagaatt attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat
     1621 ggatagtagg ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga
     1681 tagccactgc acatggaacc aaccttattg atttaagagg agagtacttg gatgaaacga
     1741 cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaacctt
     1801 ctgaaaagta tcaaaaagag tggccccatg agcattcatc atcttctgtt ttccatctca
     1861 ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat
     1921 taggtggctg tattgatacg atcagtcatt taatcggtac cccttatgga gatgtccaat
     1981 cttttaaaag aaagtataat gatggaaagc cgatcttgtg gtttctagaa aattgtgagc
     2041 tatcgtcagt tgatttaaga agatcacttg ttcagatgaa actggcagga tggtttgatg
     2101 gcagctcggg gatcatgttc ggtagaagtg cagcagagga tttgttagat tataaaatga
     2161 aggatgtata tgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt
     2221 gtggtcatgt accgccacaa ctgacattga tcaatggtgc ctatgcagaa gtagcggtag
     2281 aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag
     2341 tttatcaaag accaacaaat ctaaatagaa aagaaaatga aagaagctga cagtattttg
     2401 ccagcttctt tttgtatgat ctattagaaa ttttaattat ccataactat cacattgatc
     2461 agctgtttca catcatttgg attacctgta ggaatagcta ctacagatgc attatcaata
     2521 atttcttgag ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc
     2581 caatgtgaag gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa
     2641 actccctggt aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc
     2701 cctgcatcat aaggggcgat aggtgttcct gcaggataat gaataacatg tagttcgaaa
     2761 tcacccgatg tatctctaaa agtattacct ctaattgcta gccaaacacg tgcgtactca
     2821 atttgtagat cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaagtt
     2881 gtgtctattg ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta
     2941 ttgggctctg agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc
     3001 tgagtacttg atttttcagc tgaactggtt tgtttgattg aacttttgtc tgtggtcgca
     3061 gatggatcac tattttcttt actaggagac gtacatccta caaggaataa agagaccaac
     3121 aataaaataa aacctaactt tttcatataa aatacctcat tattttttta ataccagtat
     3181 agcataatcc tgaatttttg aatccgtaaa accttgtct
//
LOCUS       DAJSVP010000155         3160 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSVP010000155 DAJSVP010000000
VERSION     DAJSVP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.161"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..937
                     /locus_tag="ON449_002651"
     CDS             <1..937
                     /locus_tag="ON449_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3935165.1"
                     /translation="FLIQNSKYSKMKDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLE
                     FTQADLMDKLNCYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISA
                     DDLFIQNSFDDDLSETIDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDT
                     KRHLKDTETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTT
                     IHNAKHKAEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVY
                     VKNWFIEKAIAAKLANEQRETFNELPTVSTENWLE"
     gene            1795..2313
                     /locus_tag="ON449_002652"
     CDS             1795..2313
                     /locus_tag="ON449_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR3935166.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1168 a          459 c          567 g          966 t
ORIGIN      
        1 ttttttaatt cagaattcta aatactctaa aatgaaagac agctcaaaaa tagcctatat
       61 gctttttaaa aatgaatttc gtagagcctt acatgaaaaa tggatcgacg atgatggtct
      121 attatatttg gaatttacac aagcagacct aatggacaaa ttaaattgct accaaggcaa
      181 agtaaaaacc attttaaacg agttagaaac atataatttg atttcagtcg taaaaggaga
      241 atttaaccca aaaacgaaaa aaaatgaaaa aaataaatac tatttacttc aacctgaaat
      301 aagtgccgat gatcttttta ttcaaaattc tttcgatgac gatttaagcg aaactattga
      361 taaatcaacc tcttctcgga atgcggaaat cgcacgtcga gaaaaaacag ctgaaaccct
      421 tgataacact ggaaatgcgg aaatcgcaca agattattat attaataaat ctttagacac
      481 taaaagacac ttaaaagaca ctgaaacaga ccaattacaa aatcaagttt tgctagataa
      541 cttcgttgaa atcatgaaag aagacagcat aaacacattt gtaccagaaa aggtattaag
      601 cttgataaaa acattttcta acacttacga agaagcccaa caaaccgtca ccacaataca
      661 caacgcaaaa cataaagccg aggaacttga gaagacggta gttgtgtatg aagaacttga
      721 atcttacgga attgatgcgg ataaaggatt atatatgacg ttgctgaaag cttatcaaaa
      781 acaaaagact gagaaagtaa ataatttgca gaatttgatt tttgtgtatg taaaaaactg
      841 gtttattgaa aaagctattg cggcaaagct tgcaaacgaa caaagagaaa catttaacga
      901 attaccaaca gttagtacgg aaaattggct agagtagttg ataaaaagcg aacactataa
      961 aaatgatcaa ttttaaatta gaactttttt acaattttaa ttttattaca aatgttacaa
     1021 ataaatagtt attattccgg agtttttaga ttaaaagttt atgaaaacaa tcacaaacgt
     1081 aggttttaat gtcataatcg ttagatattt tagttatttt tcattcaaaa aaacagggaa
     1141 ctagtgtttg catatgtatt tttttgagtt ataataattc atgaagagag gtaattcact
     1201 tatttaccca agctgaaaag cctgatatta cttcttttca ggcagtgctg ctaatattca
     1261 attacacttt tttgtataaa tttagttgtt ttaaatttaa ttgtctgcta tgcttgtgat
     1321 gaattgattt tcgttctgca cttttggacg ataaatttgg acaattgaaa atacaaaaaa
     1381 aaagaaaggc cctgtgagac tcggaaggct accaaccttc gtcgacgtcc acaggtggca
     1441 ttaagatcac tcccttaatg cgtttacctt tcagtgttac ggtattaccc gtaacggatt
     1501 tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata
     1561 cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc gtgtggctct
     1621 ttgccgtatg acgaacgctg aaattaggag acgttgggat attttataaa tcattcacat
     1681 atgagttaga cagtcaatgc atatcagata tgcattgtag aacggattaa aatatcgtgg
     1741 atgaaattat agatgaatta aaggagacaa ttaggaattt atggaggtaa tataatgaga
     1801 acatataaaa ataaagaaga attaaaaaaa gaaataaata ggacttatac taaatatatt
     1861 ttagaatttg aaactattcc tgaagaattg aaggataaaa gagttgagga agtggataga
     1921 acacctgctg aaaatttagc atatcaagtt ggttggacga atttaattct aaaatgggaa
     1981 aatgatgaaa gaaatggatt atctgttaaa acgccttctg atcaatttaa atggaatcag
     2041 ttaggagagt tatataagtg gtttacaaat acctatgcac atctaccatt gaaagaatta
     2101 gaggaaattc tcaatcgtaa tattgatgat attaacatga tgattgattc aatgagtgat
     2161 gaagatctat tcactgcaca caaaagaaaa tgggcagacg aggcaaccaa aacggctgta
     2221 tgggaagtat ataaatttat tcatgtaaat acagttgctc catttgggac ttttagaaca
     2281 aagataagaa aatggaagag attagctttg taaattcccg tttactgcta agcaattgtg
     2341 gaaaggagac ctcttgtatg ttaacagtaa gaacatacga ctacataagt tctttgctaa
     2401 ctgccggttt ttattgggtg aatgcaagtg cactccccta gaatgccgta gagggtgcat
     2461 cgttagattg tatcaatttt ggttggccaa cagataaacc atgactcact tcgataaaat
     2521 gagattatgg tgccatatcc gctacaaagg gacttaggag actagcaggg gaaagatttt
     2581 tttatttttg ggatgttttt cagaccgccg atagtaccga caattccctt taaatatgga
     2641 atctcaacaa cccacatgaa gggtaaggca agtccggtaa ttatatcttc tgactttggg
     2701 ttaggaagaa ttgaaattgt taagaacgat tcaacgctta tggtttataa tttggagata
     2761 gcctttgtta atatgctaaa gccaacatac gatacagatt ttgagcagtt tattctaacc
     2821 atcaaagggt atgtggccta tcgcctatcc tattactgat actgaagaga tcttaaataa
     2881 attctatacg ggatttgaag atcctgaagg tgaaatcgtc atttatcaga ataaataaat
     2941 caaatagtag aagttcaaag aatgagggga acattagttt ttattgctta gtttaatccc
     3001 agtaccgatt tctgcaagat gtaaaaggtc tttcatttca taagttttca gaagcaagtt
     3061 catacgcact aactcacttt cgattgagta gcaatcgtca acaaacttta ctatcagctg
     3121 ttatttttat tctttcgtgt tatctaaata ccatcaattt
//
LOCUS       DAJSVP010000156         3105 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSVP010000156 DAJSVP010000000
VERSION     DAJSVP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.162"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(322..936)
                     /locus_tag="ON449_002653"
     CDS             complement(322..936)
                     /locus_tag="ON449_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3935167.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1542..1919)
                     /locus_tag="ON449_002654"
     CDS             complement(1542..1919)
                     /locus_tag="ON449_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935168.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2219..3100)
                     /gene="galU"
                     /locus_tag="ON449_002655"
     CDS             complement(2219..3100)
                     /gene="galU"
                     /locus_tag="ON449_002655"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3935169.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          939 a          649 c          483 g         1034 t
ORIGIN      
        1 ggggaagact ctttcatcaa cgtgtattaa gagacaagaa agaatccata tggattcttt
       61 cttgtctctt tttccactac tttttatttt tctgacacta catattctta taactcattt
      121 ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc
      181 aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta
      241 ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga
      301 actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt
      361 aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc
      421 tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac
      481 caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga
      541 caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag
      601 ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt
      661 gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc
      721 ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa
      781 tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg
      841 tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc
      901 caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact
      961 tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac
     1021 agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca
     1081 ataccttgac atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta
     1141 gaattttcac cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca
     1201 aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta
     1261 agggtcaggg agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc
     1321 ttatccaagt gcaagcaaga aaatacaaaa aaacattggt atgatttata tatatataaa
     1381 taacataaaa aaaggaatgg cttatcccga tacaacaagc attcctttaa aaaagattct
     1441 tttatataag tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt
     1501 tatccattat tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt
     1561 cttctacttt gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg
     1621 cctatccgag actttctttt catatatggg atataaatga ttagatacag tacagcaaaa
     1681 aataaagcaa atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca
     1741 accccaacgg atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg
     1801 cgaaacatga taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct
     1861 tccatcagcc acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata
     1921 gtgaacacac accttttttt ataattaaaa aattcgcctt tttattctac tatgaatctt
     1981 ctggcttggc tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat
     2041 taaaccatta gcagaatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt
     2101 ttccacgact caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca
     2161 caagctgccg ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt
     2221 aactttctgt tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa
     2281 tttccgaatg ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta
     2341 cgtcgtatct ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct
     2401 gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct
     2461 caaatatttt aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc
     2521 ccatttcagg tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt
     2581 taggatcaat aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag
     2641 cggaagcttg tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt
     2701 tcatcaaatc atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt
     2761 gaagcacggc atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt
     2821 gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt
     2881 ccaattcgat atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga
     2941 tcaagatgtc ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt
     3001 tatccacgat tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc
     3061 ctagcccagc ggcaggaatg actgcttttt tgaccttcac ttgct
//
LOCUS       DAJSVP010000157         3072 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSVP010000157 DAJSVP010000000
VERSION     DAJSVP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.163"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(334..696)
                     /locus_tag="ON449_002656"
     CDS             complement(334..696)
                     /locus_tag="ON449_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935170.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ON449_002657"
     CDS             complement(770..1150)
                     /locus_tag="ON449_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3935171.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="ON449_002658"
     CDS             1214..2407
                     /locus_tag="ON449_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3935172.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="ON449_002659"
     CDS             complement(2484..3068)
                     /locus_tag="ON449_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3935173.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          505 g         1022 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tg
//
LOCUS       DAJSVP010000158         2949 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSVP010000158 DAJSVP010000000
VERSION     DAJSVP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.164"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(266..466)
                     /locus_tag="ON449_002660"
     CDS             complement(266..466)
                     /locus_tag="ON449_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3935174.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="ON449_002661"
     CDS             complement(771..2003)
                     /locus_tag="ON449_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3935175.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="ON449_002662"
     CDS             2257..2826
                     /locus_tag="ON449_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3935176.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT          929 a          548 c          546 g          926 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagatt tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt gcttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaa
//
LOCUS       DAJSVP010000159         2945 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSVP010000159 DAJSVP010000000
VERSION     DAJSVP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.165"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="ON449_002663"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="ON449_002663"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3935177.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="ON449_002664"
     CDS             complement(1061..1981)
                     /locus_tag="ON449_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3935178.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="ON449_002665"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="ON449_002665"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3935179.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          823 a          601 c          509 g         1012 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaact
//
LOCUS       DAJSVP010000160         2931 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSVP010000160 DAJSVP010000000
VERSION     DAJSVP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.166"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON449_002666"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON449_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3935180.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          846 a          572 c          446 g         1067 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta c
//
LOCUS       DAJSVP010000161         2866 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSVP010000161 DAJSVP010000000
VERSION     DAJSVP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.167"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..685
                     /locus_tag="ON449_002667"
     CDS             29..685
                     /locus_tag="ON449_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3935181.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            685..2061
                     /locus_tag="ON449_002668"
     CDS             685..2061
                     /locus_tag="ON449_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3935182.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2347..>2866)
                     /locus_tag="ON449_002669"
     CDS             complement(2347..>2866)
                     /locus_tag="ON449_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935183.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1088 a          385 c          509 g          884 t
ORIGIN      
        1 cttatcaaaa ataagttagg agaaaataat ggatatatta gaaataaaga atgtagcata
       61 tagttatgca aattctaaag aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg
      121 gaagttttat gcgatagtag gaaagtcagg aacaggaaaa tctacacttc tttccttact
      181 tgctggactt gataaacctc aaacaggaaa aatattgttt aagaatgaag atatacaaaa
      241 taaaggatat agtaatcata gaaaaaataa tatatcttta gtatttcaaa actataattt
      301 aatagattat ttatcaccaa ttgaaaatat tagattagta aataaatcag cagatgaaag
      361 tatcttgttt gaactaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc
      421 tggtggacag caacaaagag tagctattgc tagagcattg gtatcagatg ctccaataat
      481 actagctgat gagcctactg gtaatctaga cagtgttact gctggagaaa taattaatat
      541 attaaagaca ttagctaaag acagaaataa atgtgtaata gttgtaacac atagtaagga
      601 agtagcagat tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa
      661 taaaatgaat ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa
      721 agtttaaaat cattaattat catattagtt attttatcta tgtcaacttt aagtctcatt
      781 agtttatcta ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca
      841 aatagttttt ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga
      901 aatgtaaaag gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta
      961 aaaagaataa acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact
     1021 cttgcgaatc aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg
     1081 gttaacgact caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga
     1141 aagcatttag aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa
     1201 aataacctta aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat
     1261 gagaaaggtg cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat
     1321 agcggtgtag taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat
     1381 acagctgcta aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc
     1441 tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata
     1501 aattggagag aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct
     1561 atatcaggta tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga
     1621 gttgtagttt cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta
     1681 ttactatcat taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta
     1741 tttatatcaa tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag
     1801 cttgggaata atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa
     1861 tctgcatcta gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca
     1921 agtttagata tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta
     1981 gtacttcttg tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa
     2041 ttattaattg acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa
     2101 tataaaccgt gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt
     2161 tctaaaataa gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct
     2221 gagaagttag ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa
     2281 gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat
     2341 aaccatttag ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac
     2401 ggttatggca ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga
     2461 gaccgggagg cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac
     2521 atcgcagact gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt
     2581 atctacttcg tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca
     2641 ggtggctaat ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt
     2701 ttccaagcct cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat
     2761 tcgttcaatc gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag
     2821 atgttccact tcatccatca gatctcccca aacatgtctc gtgatg
//
LOCUS       DAJSVP010000162         2862 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSVP010000162 DAJSVP010000000
VERSION     DAJSVP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.168"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..707)
                     /locus_tag="ON449_002670"
     CDS             complement(<1..707)
                     /locus_tag="ON449_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3935184.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESL"
     gene            1506..1787
                     /locus_tag="ON449_002671"
     CDS             1506..1787
                     /locus_tag="ON449_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935185.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            1789..2205
                     /locus_tag="ON449_002672"
     CDS             1789..2205
                     /locus_tag="ON449_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935186.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2366..2632)
                     /locus_tag="ON449_002673"
     CDS             complement(2366..2632)
                     /locus_tag="ON449_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935187.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(2590..>2862)
                     /locus_tag="ON449_002674"
     CDS             complement(2590..>2862)
                     /locus_tag="ON449_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3935188.1"
                     /translation="LDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYRLR
                     KLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          928 a          626 c          397 g          911 t
ORIGIN      
        1 tccaaacttt cttgaaccat ctcataccaa tcagtcgctt ttccacggcg caattgtaac
       61 tccaaacgcc accacgtctc gacttcttta ggtacaattt ttcgctttgc ttcctgttcc
      121 aacttcttgt tgtacatccg aatttggcgt tcgctggcac gactacccca ataagccgtt
      181 tctagcttac cagagcgtcc ataaattggt ttgaaggaca cagggtcaac tacacgatat
      241 tgtgtaataa actcatcagg cacatcaata agatcgcagg caacatccgc tcgtgaaaaa
      301 tggggctcta aaaataaatc atgaataaat tttttcatag agcttgctaa aaattgacta
      361 attttattgg ggttaaagtc aatccgccct ttaccctctt gccatttcaa aatttcgata
      421 taggcaatat tttcttcgtg ttgatctacc aattgccaac cattattccc aacagctttt
      481 aaatgaccat taccttcgtt cactcgcatt aattgctcaa aatctaaaat caacacatca
      541 ttttgagtgt gataataaat attttcattc aaagttccga caatcgtaat tcggtcaatc
      601 gaccaatcca tttttatcat ttttacgctc tcaccgacat ttaaccgcct gttagagggg
      661 gctgttaatt tttcccactc attcacaatt gttctttttt tacccaaatt ttgttcctcc
      721 tcaaagaaaa aaagagattt ttggaaagaa tttccagagt tcacgtaact ctaaaaatcc
      781 acatccaaaa atctctagct tgaagaaacc taaaaatatt cgtataatga acttgagtta
      841 cggaactagg ctagtagttt ccgtaaaggt cgagagtgtt agcgcactgc tcgactattt
      901 ttttgctctt ttttctcgtt ttctttgatt atagcactcc tcttaaaaaa ataaaaagac
      961 ctcagggaat ttcattcccc gaaccccttg agcctcctcc tgccgaaatc atttcacgct
     1021 tccgctcttc atttcattca gttctcggct ttcacaatct aacacgccgt ttcgctacca
     1081 gtcgccttac aggctccatt ctcgcttctc ttacgtgttt taccgattgt gccgacaact
     1141 aaacgaaatg aacgctaaaa cgtcttagaa cgcaaaatac acgcttataa aatcatttca
     1201 tcttgattat atgctcatca taatcgttca atttgacatc atttccgtaa acttttttta
     1261 tttttataag cagaaaaaag tcgcacatca acattttttt aaagagcata aggaaacgtg
     1321 tacccacttt ctaaaaaaag cgggcagata ggctgtgcct atcacgtttc taagaaaaaa
     1381 atttgatatc actttttcgc cattgtgact agcttatgta tagtgtttca gcaaaaaaca
     1441 gctatacaaa tactatattc gtatgatata cataaattat acacaaatgc gaaaggagtt
     1501 tttctatgga cattttagtt cggaagattg attcaaaggt tattgcaaaa atagacaaac
     1561 gttgccaaga actcacaaat aaaactggca aaaaatggag tcgaaataaa tatctaaaag
     1621 ttttaatcga aagtgatttt gaccgtcaac ttacgactta caaaaaagac caattcgatt
     1681 tattactcga aaaatttatc gctattcaga caaaaaacac tcaagtacta aacgactatg
     1741 ttcatacaaa taatcaactt attgaaacac ttattggagg aaaatagcat gagaaaaact
     1801 tctgttcaaa aacacgttac cctttatccc agtcacgtgg acgttttaga ttccattatc
     1861 gaaagaaatc cagatatcaa caattactcg gaagccattc gttttttatg tatgagttat
     1921 gaacctgata ccaacacgac tactaccagc aatcaagcca aactaaatgc tatgggaaaa
     1981 gaaatttcca tcatgtccga aatcattgca gaatttgctg atttatatct aaaagataca
     2041 ggcgttctta ttggcacaaa ttctaaagtg tatcaagaag tgaaagaaaa aaccgaagct
     2101 aaactaaatc cgtcgaatcg aaatcgacca aagaatcatc aaaaagaaac gagtcaaaca
     2161 gtcacttcta gaaatttcga cccaacaaat tttctaaaac gctaaaaaat aaaacgagaa
     2221 tttttttatt tctcgtttta ttttttagga aagccggtct tacagcaaga tttgcaagtt
     2281 accggtaact tgcggaaaag aagaagccgc tcacgcgact tcttcttttt gcattttctg
     2341 aaagctacca caattcctaa cttcgctaat tatattctcc atgaaattcg ctccatgtat
     2401 caaaaatcac atctgaaatt ccatcttctt catcaaataa ttgctgcatt tttttcaatt
     2461 taccctttct ctctggcgta atatttttgt attttagtaa attctcatcc aacaaatcca
     2521 gcttgaccaa ctgaatagca ctctccaatt tttctagatt ttgataatac tcgtatggaa
     2581 atttctcagt catttttctt cctcctctgt cacatctaaa cctaaaagcc atgtatccaa
     2641 ttctcttttt aattcgtctg cagactcctc aaatgtttct cgtaaatgat tcgtcaattc
     2701 atcattttga ctttcttgct tcagcaattt ccttagccga tatttcaagt cacgactaat
     2761 agttgaccac tcagaatggt catacaacaa cccctttatc atcactctat ccaacgtttt
     2821 tacatcttca ggaaaataat gcggactagc aaaactatcc aa
//
LOCUS       DAJSVP010000163         2822 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSVP010000163 DAJSVP010000000
VERSION     DAJSVP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.169"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            95..1054
                     /locus_tag="ON449_002675"
     CDS             95..1054
                     /locus_tag="ON449_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HCR3935189.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ON449_002676"
     CDS             complement(1487..2059)
                     /locus_tag="ON449_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3935190.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="ON449_002677"
     CDS             complement(2075..2680)
                     /locus_tag="ON449_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR3935191.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          886 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 gt
//
LOCUS       DAJSVP010000164         2650 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSVP010000164 DAJSVP010000000
VERSION     DAJSVP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.170"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            36..197
                     /locus_tag="ON449_002678"
     CDS             36..197
                     /locus_tag="ON449_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935192.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="ON449_002679"
     CDS             210..713
                     /locus_tag="ON449_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3935193.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="ON449_002680"
     CDS             773..1162
                     /locus_tag="ON449_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3935194.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="ON449_002681"
     CDS             1149..>2650
                     /locus_tag="ON449_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3935195.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DAJSVP010000165         2649 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSVP010000165 DAJSVP010000000
VERSION     DAJSVP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.171"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            86..1093
                     /locus_tag="ON449_002682"
     CDS             86..1093
                     /locus_tag="ON449_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3935196.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1105..2109
                     /gene="neuB"
                     /locus_tag="ON449_002683"
     CDS             1105..2109
                     /gene="neuB"
                     /locus_tag="ON449_002683"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3935197.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2106..>2649
                     /gene="neuC"
                     /locus_tag="ON449_002684"
     CDS             2106..>2649
                     /gene="neuC"
                     /locus_tag="ON449_002684"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR3935198.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILN"
BASE COUNT          971 a          335 c          525 g          818 t
ORIGIN      
        1 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
       61 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      121 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      181 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      241 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      301 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      361 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      421 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      481 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      541 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      601 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      661 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      721 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      781 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      841 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      901 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      961 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1021 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1081 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1141 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1201 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1261 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1321 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1381 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1441 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1501 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1561 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1621 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1681 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1741 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1801 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1861 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1921 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1981 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2041 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2101 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2161 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2221 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2281 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2341 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2401 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2461 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2521 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2581 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2641 ttttaaata
//
LOCUS       DAJSVP010000166         2643 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSVP010000166 DAJSVP010000000
VERSION     DAJSVP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.172"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="ON449_002685"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="ON449_002685"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3935199.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DAJSVP010000167         2621 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSVP010000167 DAJSVP010000000
VERSION     DAJSVP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.173"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..553
                     /locus_tag="ON449_002686"
     CDS             <1..553
                     /locus_tag="ON449_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCR3935200.1"
                     /translation="KKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNS
                     DAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPV
                     INLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRI
                     TKTLNQILNDGEDIKWIQKQIAY"
     gene            526..1203
                     /locus_tag="ON449_002687"
     CDS             526..1203
                     /locus_tag="ON449_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3935201.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1193..2335
                     /locus_tag="ON449_002688"
     CDS             1193..2335
                     /locus_tag="ON449_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3935202.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2348..>2621
                     /locus_tag="ON449_002689"
     CDS             2348..>2621
                     /locus_tag="ON449_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3935203.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTE"
BASE COUNT          979 a          343 c          511 g          788 t
ORIGIN      
        1 aaagaaaaaa tacgttttgc aagtatttca tccagttgta accgaactag aaaatataga
       61 aaaacaaatt ttagctttga tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat
      121 tttacctaat agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa
      181 aacggatatt atttatatta cgaatttaga gccggaggaa tacctgacta tattaaaaga
      241 gtgcaaattt atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat
      301 accagtaata aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa
      361 tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt
      421 tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat
      481 aacaaaaaca ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca
      541 aatagcttat taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca
      601 aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat
      661 aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg
      721 gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga
      781 aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca
      841 agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct
      901 gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt
      961 gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt
     1021 ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag
     1081 aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca
     1141 cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc
     1201 tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc
     1261 ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat
     1321 tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat
     1381 acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt
     1441 tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga
     1501 ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc
     1561 tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc
     1621 tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc
     1681 agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg
     1741 gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc
     1801 tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc
     1861 accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg
     1921 tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt
     1981 caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga
     2041 tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga
     2101 tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg
     2161 gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa
     2221 tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat
     2281 gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg
     2341 atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact
     2401 cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg
     2461 aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaacag aggcaaaaaa
     2521 tttgtttgaa ataaattcag tttttgtttg tacatcccct ataacccatg aatttattat
     2581 aaaagaggct ttaaatttag attgtaatat ctttacggaa a
//
LOCUS       DAJSVP010000168         2400 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSVP010000168 DAJSVP010000000
VERSION     DAJSVP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.174"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            207..2069
                     /locus_tag="ON449_002690"
     CDS             207..2069
                     /locus_tag="ON449_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3935204.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ON449_002691"
     CDS             2069..>2400
                     /locus_tag="ON449_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3935205.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSVP010000169         2376 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSVP010000169 DAJSVP010000000
VERSION     DAJSVP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.175"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            144..494
                     /locus_tag="ON449_002692"
     CDS             144..494
                     /locus_tag="ON449_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3935206.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="ON449_002693"
     CDS             908..1726
                     /locus_tag="ON449_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3935207.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="ON449_002694"
     CDS             complement(1799..2158)
                     /locus_tag="ON449_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3935208.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          787 a          344 c          448 g          797 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctcta
//
LOCUS       DAJSVP010000170         2238 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSVP010000170 DAJSVP010000000
VERSION     DAJSVP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.176"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..1556
                     /locus_tag="ON449_002695"
     CDS             24..1556
                     /locus_tag="ON449_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3935209.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            1734..1955
                     /locus_tag="ON449_002696"
     CDS             1734..1955
                     /locus_tag="ON449_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3935210.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          752 a          237 c          429 g          820 t
ORIGIN      
        1 tagaaagttt ggagccatat gatatgataa ttaatcgtaa acaaaatgct aaaaggaata
       61 taattactgg aacaattttt aagttatatc agattatcat tccctttttt atgcgtacac
      121 ttatgattta tcagatggga atggagtatg ctggattgaa caacctgttc acatctatat
      181 ttcaaatgtt aaatttagcg gaattgggaa taggtgctgc actaactgta agtatgtata
      241 aacctatagc agaagacaat agaaaagaga tttgtgcttt actgaacgca tataaaaagt
      301 acttttcaat tattggatgg atagtgttta cattaggatt gttatgcatt ccttttttga
      361 gatttttagt taaaggttcg attcctggaa atttaaatat gacagttttg tatttaatgt
      421 atttgataaa tactgttttg tcgtattggt tattttcata tagaaagagt ctaatatatg
      481 cacatcaaag aaatgatgta attaacgtgt ctatgctaat tacttttaca ttccaatttg
      541 taatgcaggc atatgttctg attttcttaa aaaattacta tttgtattta ttggcttcta
      601 ttttgggaca aatagttctt aacttaatgt gtgctgttgt ttctcaaaag atgtatcctg
      661 attatactcc tgttggtgag ttagagaaag aaactattga cgatattata aaaaaagtaa
      721 aaggattagt tacaaacaag gtaggcggta caatattacg atcttcagat tcaattatta
      781 tttcatcttt cttaggtctt tcagtacttg cgatttatca gaattattac tttattattt
      841 ctgccataat gagtatattt gcaataattt atgaatcatg tatagcaggt ataggtaaca
      901 gtcttattgt agaaagtcaa gaaaaaaatt atatagattt ttgtgatatt acaacaatta
      961 taggttggtt tctggcaatt agttgtgcat gctttattac attatttcag ccgttcatga
     1021 ttatctgggt gggaagtgat aatcttatga gtatttcatt tgttatattt atgatagttc
     1081 attttttctt atatgaaatt gatcagttaa ttgggttata caaagatgct gctggaatat
     1141 ggtacgcaga caggtttaga ccattaatta cagcaatgat aaacattgta ttaaatatca
     1201 tcctcgttaa ttggttcgga ttatatggag ttttggcggc aaccattgtt tcagtattgt
     1261 tggttgattt accctggtta ttatataatg ttttccaaaa tatatttaaa tttgaatcgg
     1321 caggtaaata tgtttggaag ctcataagtt tgtgtgtaat agcttttgtt gcttcttatt
     1381 taagttattt tgcttgttcg tatgttaata tcggaaattt caaagtgata agtttagtga
     1441 taaatttagt tatatcggtt gtcatatcga ttttagttat tcttctgttt aatatccgta
     1501 ataagaagtt taagtatatt ttgaaagttc ttacgtcggt attggtaaaa agatgagaag
     1561 agacatcaga atgatatata aagtaactac aatttttagg ctgccgaata ggcgaacgat
     1621 gtatagtttt tgttccaacg aagactcttg ttgaaattgg agatgatatg aaggagttac
     1681 aattttgact catgtttatg attgatcggt tttaaaagta gtatggagaa gctttgggat
     1741 catcggagag gatcaaaata ggtaataatg tttttatagg aatgaagtct agaatactaa
     1801 agggtgtaca tataggaaat aatgtagtta taggtgcaaa tagtttagta aacaaggacg
     1861 tatcagacga ttgtgttgtt gcaggaaatc tagcaaaagt aattatgtcc ctagatgaat
     1921 attataaaaa gataaacaat atgaagaatc cgtaaaactt gtgaaactat accgagaata
     1981 atatgggaaa aatattgatg aaaaagcatt atatgagttt ttagggctgt ttacagatgg
     2041 agatgacaat ttgtcgaaga gttgggagca atgcaaaatt tagtagggga ttataaacag
     2101 actgacaagg tatttaaaga acatgcacaa gtatttcata atattgaaga atttttgaat
     2161 actgtgagat aggagttgct acattgagaa aagtatgtat ttttccaatg taaaagatta
     2221 cgagtttgaa aacgacac
//
LOCUS       DAJSVP010000171         2232 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSVP010000171 DAJSVP010000000
VERSION     DAJSVP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.177"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(972..1262)
                     /locus_tag="ON449_002697"
     CDS             complement(972..1262)
                     /locus_tag="ON449_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935211.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1470..2162)
                     /locus_tag="ON449_002698"
     CDS             complement(1470..2162)
                     /locus_tag="ON449_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3935212.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          630 a          424 c          331 g          847 t
ORIGIN      
        1 atttaataat ttccatagca taaagtgttt agaaaagaac attaagaata gttctataat
       61 aattcttgaa ctccatgtct tataatgttc tatcgaagca cccaaatagc caagttcatc
      121 taatctactt gcataaacta cgtcatccgc tctcatagtc ggcatataca tattaaagag
      181 taaaaagata ccccaaatcc ctattagtaa caagatcagt tctttgttat tcgttatttt
      241 tctattatgt ttgttcattt ttatactcct tcttatattt cctaatagag tataacatag
      301 ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata
      361 ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga
      421 aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact
      481 gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt
      541 ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga
      601 ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac ataccggtac
      661 tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc
      721 tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt
      781 tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata
      841 cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt
      901 caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag
      961 ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt
     1021 ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat
     1081 tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct
     1141 aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc
     1201 aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc
     1261 ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct
     1321 gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc
     1381 gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg ttgcttcttt
     1441 actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc aacaatttgc
     1501 tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt ttgaatatat
     1561 tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt atctgttaac
     1621 tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt caaaccattt
     1681 acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc tgtccaatct
     1741 gcattcagat gattgatttc atcatttttt tggttgatct catctagttt gacattgatt
     1801 tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc taagttttgt
     1861 ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt ttggtattct
     1921 tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga
     1981 tctgtaagcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag attctcattg
     2041 attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta ccgcgtgcct
     2101 gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa tttctttttc
     2161 atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat ctcaaacaca
     2221 atgtcaagcg tt
//
LOCUS       DAJSVP010000172         2210 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSVP010000172 DAJSVP010000000
VERSION     DAJSVP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.178"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            386..841
                     /locus_tag="ON449_002699"
     CDS             386..841
                     /locus_tag="ON449_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935213.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(1003..2175)
                     /locus_tag="ON449_002700"
     CDS             complement(1003..2175)
                     /locus_tag="ON449_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3935214.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          713 a          441 c          394 g          662 t
ORIGIN      
        1 tgcatactta ataacagaac tatagcaaaa aatcccgtgt atcaaaacgg gattagatta
       61 agtttcctca atcactattc ataacacaat tattataaca agtaagtaaa caattgaaaa
      121 ggacatttat tgaaaacggt tgttttttac ttaaggagga aatttagtga aaaaattagt
      181 tgcattgttt ttggtcgcag gaagtttgtt gagtgggtgt ggtgctacta cgtctactac
      241 tcaaagcaca agttcagctc aggcttccag cagcagtacc acgatctccg aaagtacggc
      301 agattcatcg aaagaacaag aacaaattga aaagaaaaaa gaagaagaga aacaaaaagc
      361 ggaaaaagct gcactagaaa aaaaagtgac agaagcggaa tctgctttgt cacaagctga
      421 agcaaatcct acaaaagaaa actatgcaac tgcttcagca gctatccaag cgctacctga
      481 aaacaaacaa gagttgacaa gccgtcttgc tgcaattgat tccacaatca aagcaaaaga
      541 agcagaagaa gaacggaaaa agcaagaaga aattgcacga caacaagcag aacaagcgca
      601 acaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaagcag cagctgaaca
      661 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
      721 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
      781 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
      841 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagctggc
      901 gttccagata gtaaaccaaa aatagcagtt tacacgtcca tttcgtcagc ttctctcttt
      961 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     1021 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     1081 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     1141 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     1201 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     1261 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     1321 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     1381 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     1441 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     1501 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     1561 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     1621 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     1681 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     1741 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     1801 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     1861 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     1921 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     1981 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     2041 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     2101 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     2161 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag
//
LOCUS       DAJSVP010000173         2202 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSVP010000173 DAJSVP010000000
VERSION     DAJSVP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.179"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            267..1637
                     /locus_tag="ON449_002701"
     CDS             267..1637
                     /locus_tag="ON449_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR3935215.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1658..1942
                     /locus_tag="ON449_002702"
     CDS             1658..1942
                     /locus_tag="ON449_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935216.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          756 a          319 c          463 g          664 t
ORIGIN      
        1 tgttataaaa gtatctgtat aatgttcagt atattttgat gaacgcatat gtagtcctca
       61 tttcttggga gttttgagac tactatatgc gttttttgtt gatttaccaa catgtttaga
      121 cattttcctc taggaaaaaa ttaactagca ccacgcgtat tgtttatatt aaattagatg
      181 accgtgataa tgcacagcaa atttttgaaa gtatcaattc taccggtgaa agattgactg
      241 cctcggattt gattcgaaac ttcatcatga tggataaatc taatgaagag cagactacgc
      301 tttatactaa atattggcgt aggttggaag aagtctttga taattctaaa ggaatggaag
      361 actttttcag atactacctt gctgcaataa ctggggagta ttcagcaaag catattcttt
      421 atcaagcatt caaagactat tggcataaag agcgcgatgt atcaagtgat gccgaattgc
      481 tccaaaaatt agtgcgatat gctggttatt ttgcgagact ttactataac aaacctgaag
      541 gaaaatactc agaagtattg agcgatttcc aaagtatgga aagtatgatg cctgcacctt
      601 ttgttttggg attatctgaa tggtactatc atgaccaatt tatcactgaa gaccaatata
      661 ttgatgcgat taaggtagtg aatgattatc agcttaggag atattttaat ggggatgata
      721 caagcagagt gtcaaaagca tttccttcat atttgaaaag tgttaggaag tacgctaaag
      781 ctaatggata tgaaaatatc gttgatattg taatttatgt tcttgttact agaaatcaat
      841 caaataatat ggcacttcca tctgacaagg cattgaaatc taatctattg aatgccaatg
      901 cttatgcgat gagattggcc cgttggttac ttgaaaaaat agagaatcga gaaaattcag
      961 caacacttga tatgagtaac ttgtccattg aacacatcat gccacagact agtacttcat
     1021 attgggaaga gaaggctggt acatcggggg aagaatacac aggattagta aataccattg
     1081 gtaatttaac actcgttaca aatcctgata attcggccgc gggaaataaa gactttgaga
     1141 ccaaaaagaa aatctttgaa gatacccttc atattcgtat gaataaagat ttgtacgaat
     1201 tgactgagtg gacatcatcg gatatttctg ccagaagtga agctctaatc aatgaactga
     1261 ttactatgta tccctatttg agatcgtcgg gcgactatga acacgatggg aatagggaaa
     1321 ttttcttaga ggcgcaagga ataaaagcaa ctgggtattt gaacgaagac gagaccgtga
     1381 ttatacattc aggttcagaa atttattcta agattaaaga tattgcatct gattcgctag
     1441 atgaaactcg ccaagagctg ttggataatg gaatcattga agaaacaatt gggggactac
     1501 aatttgttca agattacact gcctcatctg tttcgaatgc agcagctctg ctattagggg
     1561 gtagccggaa tggttgggac tactggaaat atgataacgg aataagtatt aatgattcgc
     1621 taagaaataa gaaatagagc atagaggtgg tgttaggttg gataagaaca aactaagata
     1681 tgcaattctt aaattgcgtg atgaaaaaaa gaatcctttt attgagctta aagggcaagt
     1741 tccagaagaa gatattttag aacaaactaa atttctttcg cgcaatggct tactagaatc
     1801 catggtttgg gcggataaca ctgttcatat ctgggggagt gtttctctgg aaggggaaaa
     1861 atatttagtt gaaaactcag gtcttgctaa ggcgtatgca ttggcaaaag aagttaaaaa
     1921 ttggattcca ttttggggct aagataagtt tttaaacgga gaaggtagca aatcataatt
     1981 ggtttgttgc cttttttgat tgcccaaaaa gaaaaatgac aaagtataga gataaatgac
     2041 atagtacacg ggaaaaatga cattgtatca ccgacaacgt ctaaatagat gttgatatat
     2101 atacgttccg tacaaataat tataaaaatt taaatgattt gagtagaagt atcagtgaat
     2161 atctcaaggg gaatagctat ctcaagattc gattttcaca ta
//
LOCUS       DAJSVP010000174         2114 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSVP010000174 DAJSVP010000000
VERSION     DAJSVP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.180"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(119..709)
                     /locus_tag="ON449_002703"
     CDS             complement(119..709)
                     /locus_tag="ON449_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3935217.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="ON449_002704"
     CDS             1232..1393
                     /locus_tag="ON449_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3935218.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="ON449_002705"
     CDS             1412..1906
                     /locus_tag="ON449_002705"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3935219.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1899..2060
                     /locus_tag="ON449_002706"
     CDS             1899..2060
                     /locus_tag="ON449_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935220.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          758 a          331 c          362 g          663 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aaga
//
LOCUS       DAJSVP010000175         2060 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSVP010000175 DAJSVP010000000
VERSION     DAJSVP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.181"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(71..226)
                     /locus_tag="ON449_002707"
     CDS             complement(71..226)
                     /locus_tag="ON449_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3935221.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            843..1064
                     /locus_tag="ON449_002708"
     CDS             843..1064
                     /locus_tag="ON449_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935222.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1065..1322
                     /locus_tag="ON449_002709"
     CDS             1065..1322
                     /locus_tag="ON449_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935223.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1458..2060)
                     /locus_tag="ON449_002710"
     CDS             complement(1458..2060)
                     /locus_tag="ON449_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3935224.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          465 c          313 g          603 t
ORIGIN      
        1 tcatgctata atcaccttaa ttgaacaaca gtctaacagc aaaagcagtg cgccaacact
       61 aatgcttttg ctattttttt tgctcatttt cctttccgtt agcattaacc caaattgtaa
      121 taagaccaga cttactaaga cccatttgtt tcgcaatttt atcaagatta tctgccactt
      181 ccttagtaag cgttacaacg actctctttt tttcattact gatcataatt cgtatcctcc
      241 aacgactttt tcttaaaaac catagtaaca aaaaagtaac taaggtgcaa cagtttagat
      301 actaattagc gaataatagg ccttaaaccc ttggcattac tgattttgtc aaaaaaaaac
      361 gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa tgaccaataa
      421 ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa ccaaaaaaca
      481 aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg caggcgccac
      541 gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt cgctaccagt
      601 cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc cgataactaa
      661 acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag cattgatttt
      721 ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt atattataag
      781 cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg gaaggagcat
      841 aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg accagcgaac
      901 ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa gaacatctga
      961 ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc ccagaagccc
     1021 ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct aaagaaacaa
     1081 ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat tttatcgaat
     1141 tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc acaaatattc
     1201 tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca ccttcctaca
     1261 ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct tatatttttt
     1321 gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta cctacttttg
     1381 gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca caaaaaaaga
     1441 gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc accatgacga
     1501 ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag cggaaaccaa
     1561 tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt gatattctta
     1621 gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact cgttactgac
     1681 aaataactag caatttctgc accaccatga gcgataatat cacttttagc atattgaaat
     1741 ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt agcacatttt
     1801 tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata cgtcttttta
     1861 ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc aggattcaca
     1921 tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa aaactccctc
     1981 caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt gcgttcatct
     2041 ttagtttttc tactcgtcat
//
LOCUS       DAJSVP010000176         2042 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSVP010000176 DAJSVP010000000
VERSION     DAJSVP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.182"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..426)
                     /locus_tag="ON449_002711"
     CDS             complement(<1..426)
                     /locus_tag="ON449_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3935225.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ON449_002712"
     CDS             complement(535..1566)
                     /locus_tag="ON449_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3935226.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          629 a          331 c          352 g          730 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 ga
//
LOCUS       DAJSVP010000177         2029 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSVP010000177 DAJSVP010000000
VERSION     DAJSVP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.183"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(537..902)
                     /locus_tag="ON449_002713"
     CDS             complement(537..902)
                     /locus_tag="ON449_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935227.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(978..1223)
                     /locus_tag="ON449_002714"
     CDS             complement(978..1223)
                     /locus_tag="ON449_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3935228.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1293..1547)
                     /locus_tag="ON449_002715"
     CDS             complement(1293..1547)
                     /locus_tag="ON449_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935229.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1711..2016)
                     /locus_tag="ON449_002716"
     CDS             complement(1711..2016)
                     /locus_tag="ON449_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935230.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          744 a          354 c          363 g          568 t
ORIGIN      
        1 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
       61 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
      121 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
      181 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
      241 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
      301 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
      361 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
      421 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
      481 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
      541 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
      601 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
      661 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
      721 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
      781 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
      841 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
      901 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
      961 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
     1021 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
     1081 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
     1141 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
     1201 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
     1261 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
     1321 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
     1381 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
     1441 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
     1501 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
     1561 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
     1621 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
     1681 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
     1741 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
     1801 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
     1861 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
     1921 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
     1981 cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactcct
//
LOCUS       DAJSVP010000178         1993 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSVP010000178 DAJSVP010000000
VERSION     DAJSVP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.184"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ON449_002717"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ON449_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3935231.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ON449_002718"
     CDS             852..1040
                     /locus_tag="ON449_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3935232.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ON449_002719"
     CDS             1053..1610
                     /locus_tag="ON449_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3935233.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ON449_002720"
     CDS             1642..1884
                     /locus_tag="ON449_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3935234.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAJSVP010000179         1964 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSVP010000179 DAJSVP010000000
VERSION     DAJSVP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.185"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..845
                     /locus_tag="ON449_002721"
     CDS             <1..845
                     /locus_tag="ON449_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCR3935235.1"
                     /translation="KKMLIPLESEITGLLNKLTISNVDSTREKIIRTFDKINKLFKND
                     EAEFKKELLELKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRID
                     KEERQFNSEITKLMAYMQKASADVEKELYADKIKELEEKLRELETVKEDVLQRELNTR
                     AGYVYVISNIGSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTL
                     ENQLHSHFRKNEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLE
                     LTDV"
     gene            911..>1964
                     /locus_tag="ON449_002722"
     CDS             911..>1964
                     /locus_tag="ON449_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3935236.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKK"
BASE COUNT          841 a          252 c          323 g          548 t
ORIGIN      
        1 aaaagaaaat gcttatacca ttagaatcag aaataactgg acttctcaat aaacttacta
       61 tttctaacgt agattcaact agagaaaaaa ttataagaac ttttgataaa ataaacaagc
      121 tttttaaaaa cgatgaagca gaattcaaaa aagaattact tgaattaaaa cttcaagaat
      181 tagaactcaa ctattcatac atagttaaag ttaacgatga aaaagaacag caaaaagcaa
      241 taaaagaaca aatgattgaa gaagaaaagg taagacgtga aatagaacgt gaaaagaaac
      301 gcattgataa agaagaacga cagtttaact cagagataac taagttaatg gcatacatgc
      361 aaaaagctag tgctgatgtt gaaaaagaac tctatgctga taaaattaaa gaactggaag
      421 agaaattaag agaactagaa actgtcaaag aagatgtatt gcaaagagaa cttaatacta
      481 gagctggcta tgtttatgtc atcagtaata tcggatcttt cggtgaagat gtctataaaa
      541 taggaatgac tagacggtta gaacctatgg atagaattaa ggaacttagt agcgcttctg
      601 taccttttga atttgatgtt catgcaatga tattttcaga agatgcacca acattagaaa
      661 atcaactaca ttcgcatttt agaaaaaacg aagtcaataa agttaatcaa cgaaaagaat
      721 tctttaaagt atctcttgat gaaattgaaa aagttgtttt agaaaattat aacggaactg
      781 tatcttttac taaactagct caagctgaac aatacagaag atcattagaa ttaacagatg
      841 tgtagtaaaa agattagcct tcgggctttt cttttacaac gataagaaca tacattcgaa
      901 aggagataac atgaaacgag tagcattata tatgagagta tcaactgaac aacaagccaa
      961 acatggagat agtcttagag aacaaaaaga aaccctctat gaatatatcg aacaacacaa
     1021 ggatttgaaa gtagttaatg aatatgtaga cggtggtatt tctggtcaaa aaataaatcg
     1081 tgatgaattt caaaaactat tacaagatgt aaaagaaaat aaaatagatc taattttatt
     1141 tacaaaatta gatagatggt ttagaaatct acgacattat ttaaacactc aagaaatttt
     1201 agaaaaacac aatgtttctt ggaatgctgt ttctcaacaa tattatgata caactacagc
     1261 gtatggcaga actttcattg cacaagtaat gagttttgct gaattggaag cccaaattga
     1321 ttcagaacgc atcaaagctg ttatggcaaa taaaattgca caaggtgaag tagtaagcgg
     1381 gaaaactccg ttaggctact ctatagaaaa taagaaatta gtaataaatg atgatgctcc
     1441 tatcgtgata gatatattca actactttct ttctagtggt agtttaagaa aaactgttta
     1501 ttatttaggc tctcagtatg gaatcgttag agattatcaa agtgttaaaa atatgttgac
     1561 gaacaaaaaa tacattggtg aattaagaaa taataaaaat tactgtcctc ctattattga
     1621 taaaaaactt ttttatgctg ttcaaaaagc cttacctaaa aatttaaaaa caaatgcaaa
     1681 acgtgattac atttttaaag gtttattaaa atgttctgac tgtcagggat cagtagctgg
     1741 tcaaactatt aaagcaagat ataagaaaaa agacggtaca gaatctatat acgaaaggac
     1801 ttgttataga tgtgtaaaac gaagaaataa taaattacgt tgcacaaata agcgcgcttt
     1861 ttacgaaaaa aatttagaac gttatatttt tgaagctact aaacaaaaat ttgaacaaat
     1921 acaaataaac tattcaaaaa aacaaccaaa gattattaaa aaga
//
LOCUS       DAJSVP010000180         1946 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSVP010000180 DAJSVP010000000
VERSION     DAJSVP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.186"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..1217
                     /locus_tag="ON449_002723"
     CDS             24..1217
                     /locus_tag="ON449_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3935237.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1383..1805)
                     /locus_tag="ON449_002724"
     CDS             complement(1383..1805)
                     /locus_tag="ON449_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935238.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          514 a          333 c          401 g          698 t
ORIGIN      
        1 attttaggaa aaggggtata aaaatgacaa cgtatcagcg aaagttatta ttatcgacat
       61 cggcgggaat tgctttagag aatatggata ttatgttttt agctttttca ttatcttcaa
      121 tgattacttc ttttcatatt agtggtacgc aagcgggttt catttcaacg atcactaatt
      181 tggggatgct cattggaggt attttttttg gcatcatagc tgataaatat ggacgtgtga
      241 aagtattttc tcaaacggtc cttctgtttt cgattgcttc tttattgatg tattttgctt
      301 caaacatcta cttggtttat ctctttcgct ttatcgcagg tgtaggagcg ggaggagaat
      361 acggggcgtg tatgtccttg atttccgaaa gtttttcaaa aaaacaaatt ggacgagctt
      421 cttcggttgc tggtattggt gcgcaggtcg gtgcggcttt ggctgcgatt ttagctgctg
      481 tgatcattcc aacgtttggt tggaagatgt tgtatgtcgt aggtgtactg ccggtgttga
      541 tggtcttgtt cattcgtcgc ggcttgactg aaccaaagga attcaaatct gtacaatctg
      601 ccgaaaagaa aaccaaactt agcagactgt ttgaatcaaa acatcttgct tggcaaacat
      661 gtggccttag tatcatggta actgtccaaa ttgccggcta ttttgggtta atgaattggc
      721 tgccttcaat tatgcaaaat cgattagggc tttcggtttc tgggtcttca ttgtggatgg
      781 tcagtactat tttaggcatg tctttgggga tgatgacttt tggcataatc atggataaat
      841 tggggcctaa actagccttt tctttgtttt taatttgttc atccttatct gtctatctac
      901 ttgtgttagc ccatagccag tggagtttgg tagcagccgc tgtgatagtc ggctacttta
      961 tcaatggcat gtatggtgga tatggcgcag tgattagtag tttgtatcca acagaaatcc
     1021 gcgcgacggc gaataatttc attatgaacc ttggacgagc tgttggtggt ttttcttcta
     1081 tcgtcatcgg tttcttgatg gatcattata gtttaactgc cgtaatcttg tttttgagcg
     1141 gtatttacct agtcagtctg ctggtcttga caactcttac tggtgttaca gcattaaggg
     1201 aaagcttgca gcattgatag ttgagtctgt aaatgacaag aaagaggtgt ggcaaaagct
     1261 tgcatctgcg gtaataagac cagccaaatc aaaaacatga gacactgttt ccgtttggct
     1321 ggtcgtatta cttgatgcag ggcagctttt tcaactgcta atttccttct atctggcggc
     1381 tactattctt tgacgtccgt catttcaaag tactttacta ataacttagc ataacgaaaa
     1441 actgtggata cactcgtata ttgagcgtct gcataatctt ccaaattttt ctcaggatgt
     1501 tgatacatga attcaatcaa atgataatta tatgattttc tgtagaaatg aagataaata
     1561 atgtagattg ggaagttctc ctgcttttcc atagaaaagc gccgattatt ctcattcttc
     1621 aaaatgacct tatcaggata tttttggaac aattcttcta aatctgttag ataattcaag
     1681 atagacttac ttgatacctt caattcgttt tgtagttctt ctattgaaca ccaacgttct
     1741 gtttcaagca gtaaatgaat cattttaatt ttcttttgag aaacttttgt taaaaaagtc
     1801 tgcatatttt ctcctcctgt atctacttta ctatactaca tacaagaaaa aaaacggata
     1861 caaatatcat attgtcacac cgtcaatagt gcataaacct gttattattt ctcttttttt
     1921 cctaagtatt ctattgatat gaatca
//
LOCUS       DAJSVP010000181         1868 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSVP010000181 DAJSVP010000000
VERSION     DAJSVP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.187"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            34..1368
                     /locus_tag="ON449_002725"
     CDS             34..1368
                     /locus_tag="ON449_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935239.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          652 a          263 c          316 g          637 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttatttt aagtagttca
     1741 gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact
     1801 tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg
     1861 aaaatttg
//
LOCUS       DAJSVP010000182         1842 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSVP010000182 DAJSVP010000000
VERSION     DAJSVP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.188"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            572..1384
                     /locus_tag="ON449_002726"
     CDS             572..1384
                     /locus_tag="ON449_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935240.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          641 a          256 c          301 g          644 t
ORIGIN      
        1 aaaaaaagca gttatttgtt aaaatcattg atattattaa taataagtat tttttgaaag
       61 gggaaatata aaaggttttt gaaaataaat aaaaaatagt attacttgtt ctattgctag
      121 ttattgctag tctttattct tttttgtttt ttagggatgt acaaaagatc tcaaccacag
      181 gagacattgc ttttcacttt tctagaataa aatgcttgtc ctcagtattt tctggtccaa
      241 taaattttac cacatttaat agctatggcg gaggcgtgaa tttttttatc catatctaac
      301 atctttccag ccgtaatatt ttactggaca acaaacaatt tgattctaag ttatatactt
      361 actatacgtt gggatactga atatctgtac aattctagtg agctatcaat atggacaaaa
      421 gaatagatgc agccttctta ttcagctgtt tgtatagctt ttatggattg atatatatta
      481 gcgtactgca atttcagaat caattgcgtt gacgattgta ccagttgtct tgtattacac
      541 atatactctt ctttatgaaa gaaaaaactg cgtgattctt ttagctgtca gtatgagttt
      601 gctcatctat acccatattc tctccacctt tatgagtgtg attgttattg gcttcttgtt
      661 tatcggctct cttttctcta agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa
      721 tatgataaaa gctgttggtt ggaccattct tttaacagct tacgtttggt atccgatgtt
      781 acaacaagtc ctacatcaaa aaattaatcg tccttttcgc acaaaacttc aagaaagggc
      841 attaaacatt tctgatagtt taatcggtgc agtaaataat gatttagtta catttacaat
      901 gggaacaatt ggtgtggttt catttattct tccacttttc tttaaaaaaa aatttgccaa
      961 taaagaaaag attattaatt tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt
     1021 tccatgggaa tttttacaaa aaacacctat tcaaatcatt cagtttcctt ggagaatctt
     1081 gggttttcaa gtgctttttg gttcattgat cttaattata gtgtttttaa agtggaaaac
     1141 aagcaacaaa aaaagtatgt gtagccttgt aggaatagtc cttcttattt ttacagtgac
     1201 tgtagcaaca gaagcgaatt attcgcagaa aattcaatcg tataaaggtc gtttaattat
     1261 gaccaaaaaa gatgttacac tttatacaac ctcgagaact ggaggtcttt atgactatgc
     1321 accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa aagtctatga
     1381 ttgatgggag aatgcatctc ataaagcaca taattctaaa ataatctata cagtaggaga
     1441 tgcgaatgag caaaatatca cgttacctgt atttaattac tgggaacagt tatgaaagta
     1501 aatgggaaaa agagcactat agaaaatgac aatggtttag tgtctattaa aggtacggga
     1561 aaaagaacta tagttgaggt ttcatctacg tatccaacta cgatgattct atctttatta
     1621 attagttcaa taacttttat attcttattt acacgttatt taataagatg ctgcggttaa
     1681 atgaaaatag attagataga tattataaat atgttgattc tattttcata gcaatggaga
     1741 aaatacatta gcaatatata aaagaaggta tcaagagtca cggtgaattt attccgaaaa
     1801 gtgaaaagaa caaatataaa atttaataaa aatatagaac ta
//
LOCUS       DAJSVP010000183         1794 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSVP010000183 DAJSVP010000000
VERSION     DAJSVP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.189"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            245..1723
                     /locus_tag="ON449_002727"
     CDS             245..1723
                     /locus_tag="ON449_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935241.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          656 a          246 c          315 g          577 t
ORIGIN      
        1 ttttttgtgg ttgtttacgc aaatggcgca aatcttgtac attttaacac aataaaacaa
       61 aaaatataga atatttcctc ataaattttt ccattttttg caatattctc aaaaaaaaga
      121 taaaaaatga gaataactga tgaaaaaaca agcataatcc atcataaaat cagtataggt
      181 gaaaagatga actttcatct gcatatcaag taattggtat gaggcatttt ttgaggtgta
      241 ggatatgaag ttggattacc ttatagaaga ggaagataga gaaaaactgc tgctagctag
      301 atatttagaa tttgagaaaa agaattatgt agtgctgcct aagttatatg aaacattagg
      361 tttgtctaaa tttaaaacaa gaaattatat catagaatta aataaagaat ttactgaatt
      421 caaaacaaag ccagagatcc aaatttcaga agaagcagag atccatgtcg aacgtctgaa
      481 tttgacggat atcaaaaatt taagaagaat ctactttcaa cgatcaaaaa ttgctcgact
      541 gttaacggag atcgtggatt caaaagtaac gatcgaaaaa tttgcagaga atcatttttt
      601 aagcatatca agagcctatg taaaaagaag agaattagtc tcttttcttg aacagcatca
      661 aataaagtta aagaaaaaca tattagttgg ggaagaaata aacatacgga acgtattatt
      721 tggggtatat ttcggggtct ttaatggttt ttctatgcca tttagagaaa gcgtcatgaa
      781 tgaagcaaag agtatcgtgc aatattttga atatctgttc aatttaaaac tgtctgcaac
      841 aaaacgaatc aaattcaatt tgctaaccac tattagtttg ttgagggcct atgggaatca
      901 accaatcgga aaacgtttct tttcagatga atactttaag agctcagaag gaatgaagat
      961 catcaaggat ttatcacata tttcttttct tcatatccct aaagagcaga tcattgatga
     1021 gatttcttat atcatgttgt ttttgatcac ggaagaagaa tttgaattag atggcgcatt
     1081 tggacttgcg gaagctcatt ttcatgatat ccacatgatt agtcaaaaaa tgtcagtgga
     1141 attgattgat caattaccat tttcgattga aatctctgaa gaagaaagac tatatatacg
     1201 aaaagaactt cgtatgggta tgatgcatgt caatcgaaaa aattggatat tcgactttga
     1261 gatgactagc tttacgtcta aaaagcagct acagtttttc ttcgagacgt atccaatatt
     1321 cagtacagca atccaaaagg tcatacaaga gaataaacgc tatttcttgg gaaaagatga
     1381 acgtttcatg attcgcttat tttatgatta tctcttttta ttggtcggta tttgtccggt
     1441 atcatatttt gaagcaccga ttcatgtctg tattgatttt tcgcaaggat ctgcttatac
     1501 cgattacatt atttctcaag tgaaaggatt taaaaattac aatattgtga ttgagagtcg
     1561 aatcacttct cgtacgaata tttatttatc tgattgttta cttgaaaaat ttaataaaga
     1621 acagataatt tggaaaaatc caccgactcc agatgactgg gaatattttg gaaataccat
     1681 catcagagaa aaaaatagac atttaactga aaaaatcagc tagtatttat ggatggcagc
     1741 tttgtctcac atatttatta gctgatttat gtttttttca aagaaaaggt aact
//
LOCUS       DAJSVP010000184         1791 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSVP010000184 DAJSVP010000000
VERSION     DAJSVP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.190"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..189
                     /locus_tag="ON449_002728"
     CDS             <1..189
                     /locus_tag="ON449_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3935242.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="ON449_002729"
     CDS             complement(1055..1585)
                     /locus_tag="ON449_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3935243.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..>1791)
                     /locus_tag="ON449_002730"
     CDS             complement(1582..>1791)
                     /locus_tag="ON449_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3935244.1"
                     /translation="ENIKKPKIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFV
                     YNTLGGILGVILYKLILITKRDGER"
BASE COUNT          566 a          351 c          212 g          662 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt c
//
LOCUS       DAJSVP010000185         1666 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSVP010000185 DAJSVP010000000
VERSION     DAJSVP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.191"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            133..507
                     /locus_tag="ON449_002731"
     CDS             133..507
                     /locus_tag="ON449_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3935245.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="ON449_002732"
     CDS             complement(621..1250)
                     /locus_tag="ON449_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3935246.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="ON449_002733"
     tRNA            complement(1384..1456)
                     /locus_tag="ON449_002733"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          558 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtactt
//
LOCUS       DAJSVP010000186         1656 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSVP010000186 DAJSVP010000000
VERSION     DAJSVP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.192"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..504
                     /locus_tag="ON449_002734"
     CDS             <1..504
                     /locus_tag="ON449_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935247.1"
                     /translation="IQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFS
                     GKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMM
                     FEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
     gene            491..>1656
                     /locus_tag="ON449_002735"
     CDS             491..>1656
                     /locus_tag="ON449_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3935248.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAG"
BASE COUNT          533 a          215 c          270 g          638 t
ORIGIN      
        1 atacaatcaa tctttgggat agaagaagtt tttgtcggag ataaatgtat cttttttgat
       61 caacctttat tatctgatgg ttatggcata gatgaaacag atattttcgc taaagtaaat
      121 agtatatttt ctggaaaaaa agaaacttta attaagcttc atcctagagc aaggacaaat
      181 cgttatggtg aaaaaaacat attgaattcc agtttaccgt ttgaagtctt gctacttgaa
      241 aatgacttct cttcaacact tttgattagt cctatatcta caatttcttt ttcaccatct
      301 atgatgtttg aaaagacgat caactctatt atgttggcta aattaatttt aaatgaatat
      361 acagcattat ctgaagataa aaaagaaaca ttgacaaata ttgttctttt ctgtgaacat
      421 tataaccgtc tatcagaaga aaaaatttta ctcccagaaa actgggcaga attaagaaag
      481 ttggtagagt atgggattat ttaacacact ttccaagtca aaattagtta agtccagttt
      541 ctggtatact atagggaatt tttttattaa gggaatttca ttcataacta ttcctatttt
      601 tgtaaatatt actactcaag cagaatatgg tttgattaat aatttcactt catttgtatc
      661 aattttttcg attcttgttg ggctaagcct aaattcggca attaataatg caagttttga
      721 ctataaagat gacataaatg aatttatgtc atcggtactt tctctagcta cactttcgtt
      781 aattgtgttc ttattaatag ggaacatata ttttttgttt tcaaatgctt tttttgatat
      841 gaaccaactt gtatttaatt tgatgttatt acaaagtttt gctagttttg taattagtta
      901 tatccagtct tattttacga ttaactttaa ccattttaaa tatttgattt tatcttttat
      961 atctactttt ttgaatattg caatctctgt agctttgatg cagacattat attttgatga
     1021 taaatatgtt ggcagagtag tcggcggaac actgtcattt gtgttgatag gtttagtaat
     1081 ttatttaaga ataatgataa agggtgctca tttttttgat aaaaaacagt ggagatacgc
     1141 tttaaaaatc tctttgccgc taatcccaca ttccttagca aatatagttt tagcacaatt
     1201 cgatagaatt atgattaatt cttatttagg tcctgctgcg gggggaatat attcctacat
     1261 tagtaacata ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc
     1321 atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta
     1381 ttatttagta gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc
     1441 aaaatttatg gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt
     1501 aggctatttc tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa
     1561 aacatcgttt attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat
     1621 gattttcata cctaggttag gaataattgc tgctgg
//
LOCUS       DAJSVP010000187         1651 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSVP010000187 DAJSVP010000000
VERSION     DAJSVP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.193"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1380
                     /locus_tag="ON449_002736"
     CDS             <1..1380
                     /locus_tag="ON449_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3935249.1"
                     /translation="GGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIE
                     VTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKV
                     TTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEV
                     DVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSG
                     KNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLG
                     YKNRKKENE"
     gene            1514..>1651
                     /locus_tag="ON449_002737"
     CDS             1514..>1651
                     /locus_tag="ON449_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3935250.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TS"
BASE COUNT          648 a          250 c          355 g          398 t
ORIGIN      
        1 gggggcacag tagatctaac agataatgtt actaacttgc cagcgttacc agaaggaaca
       61 acagtaacag atgttactcc cggcggtaca attgatacca acacaccagg taattacgaa
      121 ggtgtcattg aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa
      181 gtgacagaca atcgttctga cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta
      241 gaaattggag gcacagtaga tttaacagat aatgttacta acttgccagc gttaccagaa
      301 ggaacaacag taacagatgt tactcccggc ggtacaattg ataccaacac accaggcaat
      361 tatgaaggtg tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca
      421 gtagaagtga cagacaatcg ttctaatgca gataaataca atcctgaagg acaaaaagtg
      481 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagattta
      541 ccaaaaggaa ctacttatgc ttggaaagag aaagtagatg ttagcacagc tggaaataaa
      601 aaaggtacta ttgtagtgac atatccagat ggatcatctg atgaagttga agtagacgtt
      661 acagtgacag atactcgttc cgatgcagat aaatataatc ctgaaggaca aaaagtgact
      721 acagacttaa ataaagagcc agatgcatct gagggaatta agaacaaaga agatctacca
      781 aaaggaacta cttatacttg gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa
      841 ggtactgtag tagtaacata tccagatgga tctaaagaag aagtagaagt tactatttct
      901 gtagtagata aaaaagcacc gaataaacct caagttgatc ctattacaga gggtgaccaa
      961 attgttactg gtaaaactga accaaatgca gaggtgacag ttacattacc agacggaagt
     1021 caacaccatg ggacagctga taaaaatggt aattttacag ttaaagttcc taaattagag
     1081 gcagggacaa aagtgatagt aactgcaact gatgaatctg gtaatactag tgaacctact
     1141 aatgtagttg tttcttccaa caaaaaagac agtggaaaaa acggtagtaa aggtagtaag
     1201 actgacaatc aaggtagcaa cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca
     1261 aaagtttttc ctaagacggg agagagtgac agtaatatct ttactattag cggtggttta
     1321 atactgttag gaacgttagg attattaggg tataaaaatc gaaaaaaaga gaatgaatag
     1381 aaaatcaatt tcttaagtct tctctgagaa gaggaagaat ctttttcttc ctctttttgc
     1441 ttttttatga tgggaaataa gtgttagtta aagatgaata tactttttat agaaaggaaa
     1501 agaatgtaat taagtggaaa aaatagtaat tattggttca aaccattcag ggctagccgc
     1561 agctcgattt ctaaacttaa gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc
     1621 agaaattagt tacttggaat gtgggacatc g
//
LOCUS       DAJSVP010000188         1592 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSVP010000188 DAJSVP010000000
VERSION     DAJSVP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.194"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            76..1542
                     /locus_tag="ON449_002738"
     CDS             76..1542
                     /locus_tag="ON449_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3935251.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          441 a          291 c          333 g          527 t
ORIGIN      
        1 ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga
       61 attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga
      121 ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca
      181 ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca
      241 acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc
      301 ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc
      361 attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg
      421 atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga
      481 catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt
      541 gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca agaatataac
      601 tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc
      661 atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact
      721 gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta
      781 ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt
      841 gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta
      901 actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt
      961 ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca
     1021 caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt
     1081 ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa
     1141 ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta
     1201 ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg
     1261 ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg
     1321 acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct
     1381 gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa
     1441 tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact
     1501 gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca
     1561 ggaattgctt atttgcagca gttcctgttt tt
//
LOCUS       DAJSVP010000189         1514 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSVP010000189 DAJSVP010000000
VERSION     DAJSVP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.195"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(290..363)
                     /locus_tag="ON449_002739"
     tRNA            complement(290..363)
                     /locus_tag="ON449_002739"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Ile,seq:cat)
     gene            complement(552..1511)
                     /locus_tag="ON449_002740"
     CDS             complement(552..1511)
                     /locus_tag="ON449_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3935252.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          449 a          298 c          273 g          494 t
ORIGIN      
        1 aatataaaag agtttaaagc gtaacacact tatggggaag tggcttgggg tgcgctttaa
       61 actcttcttt attatcatct cacaatttaa cccaaatgtc tttctattta aaaatcaaag
      121 taaaactttt caaatataca gaaatataac tatgtgaaac atcctttcat taatccataa
      181 aaggatacat aaaaaagcca ctcatttgag tggcaatgaa gaaaagcttt agcttgtata
      241 atactcttca aaaaattcta acacagaacg attcaaatgg ctacgttaat gtaccctgta
      301 ggactcgaac ctacgaccgg acggttatga gccgtctgct ctgaccaact gagctaaggg
      361 tactggttgt tgccacataa agccataaac aatcaaccag tagaatgtgt ggcaacaaac
      421 ctgttatcgc atagcttgga gtgtgactat tatgggtgat agtgaagata tgcgataaca
      481 tcactatttt atcgaatgat ttttatagtt gtcaatatag ttatgtaatg ctcctcaacg
      541 aggagctatt tttatcgttt aggaatattt aaataccaac gtttgtcatg gaaatcttgc
      601 gcaccgcctt tagtgtttcc ctctggatca ttcgttgccc gcatcatgac atagactttc
      661 ttattaggga agttacgcat gttaaaagat acatgatagc caacatttcc agaagtatta
      721 taagcttgat ttacatctga tctataaatt ccatcagctc ttactcgagc taattctttc
      781 ccagtattgt aatccataat gaagatatac tcgtatttat agttagcaat gtgccatcca
      841 gctacatgca aatttgcatt ttcgatttct ccaaactgat caatgtgggc gtaattcgtt
      901 ccatctgtca gtgtaggatt agctgcacca gctcgtgttg gatcaatgac aggcttgttt
      961 tcagaagttg ttggattttc atcggtaaat ccatgagcta aatcatatgc taatttttct
     1021 ttacttacgc ccatttcaga aagataaccg taaggatctg tatgatcgcc ccagatgttt
     1081 tgtgttaccc ataaatgcga tttgattccc ggttggttat aaggagtgtc caacgttaat
     1141 ggaataccat atttcattgc tgaatctcta gccaattcaa cgtatgcctt gtagtttttc
     1201 tcaaacgttg ctttatcatg tgtgtgttgt aactcaatct gcacaggact gttggcatta
     1261 gcatacgaac cagcaccgta ctgtacataa ccaggttgac cgacttgata aacaattccg
     1321 ccgtctccca caatataagc agtataagcg ctagtccatg aacgttgcat atactgcgct
     1381 tcattgcgtc ctgttgctgt ttcattagcc gtttcatgca gtaaaatata ctgattattt
     1441 gctacttgtg agctaccttc atttgcgccc aaattaaatt cattgttgat agtataggca
     1501 aatccgttaa ttgg
//
LOCUS       DAJSVP010000190         1502 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSVP010000190 DAJSVP010000000
VERSION     DAJSVP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.196"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..848
                     /locus_tag="ON449_002741"
     CDS             <1..848
                     /locus_tag="ON449_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3935253.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1389..>1502
                     /locus_tag="ON449_002742"
     CDS             1389..>1502
                     /locus_tag="ON449_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3935254.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          496 a          266 c          291 g          449 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctta ttaatactct
     1021 ttctcactag tgttgcatgc aacactagtg agaaagagta ttaaatatac tgcaaggcaa
     1081 gacatttggt aattgaagaa taacaagcaa aaaaatagca gtcaggtctc ttgacaaagt
     1141 atagttgcta ttcttttttt aggccctact ttaatgaaat ttttgtttgg agggaacgac
     1201 ttcattcctt tgattatttc agttgaattt aatagagaag cctggcgtct attaagaaat
     1261 acttttcttg atgagcgtca atttttttaa cggaaatcta tcctatacta aattcggttc
     1321 tgttgcaaag ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg
     1381 acagagtaat gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg
     1441 ttggttacta cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc
     1501 gt
//
LOCUS       DAJSVP010000191         1498 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSVP010000191 DAJSVP010000000
VERSION     DAJSVP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.198"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..290
                     /locus_tag="ON449_002743"
     CDS             <1..290
                     /locus_tag="ON449_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935255.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(326..697)
                     /locus_tag="ON449_002744"
     CDS             complement(326..697)
                     /locus_tag="ON449_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935256.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(699..1100)
                     /locus_tag="ON449_002745"
     CDS             complement(699..1100)
                     /locus_tag="ON449_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935257.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1114..>1498)
                     /locus_tag="ON449_002746"
     CDS             complement(1114..>1498)
                     /locus_tag="ON449_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935258.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          481 a          261 c          205 g          551 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
      301 aacaaacaaa ctcgcttgaa attcattagg tgagtttgtt tattattttt tctgcttctc
      361 gattcatcag cgccatatct gttttcgtaa tggtttcatt aggaatatag tcagcacatt
      421 ttcttttctc taacattcta tacccactat tatgtattcc taaggtattc aattccttaa
      481 ctgttgaaaa caaattccaa acggtttggt ggcttccttt tccgctactt ccattataaa
      541 taatatcata attttctttc aaatactcat cagatttttt aaaagcactg tagtatgctc
      601 ttgaaacagc agttcttcta taagattctt tagaattata tttatcatct gattcaattt
      661 gtttggaaat agataaatat tcattaaagt caaacatttt attcccccaa agttgctact
      721 acagatttcc ccatatattt atagtcagat aatacattat cgagagtatc caaaacatcg
      781 taatctgagg aaaacaattc agatgttgtt ttgataacaa agaaatacaa gtttaaatct
      841 tcatcatatt ttatattgat ttcagctttt ctttttaaaa ccgtatttat cacatcaaaa
      901 attttgccat cattacttga aattgtgtta atctcatttt ttgtagtaat tacaagttga
      961 ttatcatttt tagttttcgg agtagaactt gcatccacaa caactggaga tgctagaatt
     1021 accgatgcgc aagcaaaaat cgcagcaaaa tttattaagc taccactccg atgtgtattt
     1081 gatggtacta tatcaagcat ccgtatcgtc tccttaattt ttttccactg gtttgttgac
     1141 cttgatatca aagatttgcc ttccaatctc agttccatta aatttaacaa cagcataatg
     1201 ctctcctgct tcttcaacca agacatttct ggcgtttata ttaaagttta tatcgttaac
     1261 ttgaggaggc aagcttaccg atacttcacc cgtagaaaaa atttgttttt cattcttgtt
     1321 taaaacttca atttctacta tttttatctt ttctgtatcc tctatctcag acaccacagc
     1381 tgttacagaa aatgataaag cagtaggaat taaaggcgca ttaatagaaa gagctggatt
     1441 aattacttta ttaataacat tagtgccaaa ttgctgactt tctacatctt ctgaaatt
//
LOCUS       DAJSVP010000192         1470 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSVP010000192 DAJSVP010000000
VERSION     DAJSVP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.199"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..443
                     /gene="spx"
                     /locus_tag="ON449_002747"
     CDS             33..443
                     /gene="spx"
                     /locus_tag="ON449_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3935259.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            457..813
                     /locus_tag="ON449_002748"
     CDS             457..813
                     /locus_tag="ON449_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3935260.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          544 a          218 c          273 g          435 t
ORIGIN      
        1 gaattggatt ataattaaaa aggagtgaga aaatgataaa agtatatact tcgcctagct
       61 gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga
      121 agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg
      181 aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag ctgggcatag
      241 attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat ccttcattat
      301 tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat gaagatgaga
      361 tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc tatttacaaa
      421 acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt ctatggatca
      481 gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca attgccttat
      541 caatttgttg acttagaaga acgcgatcta agtacagaag aagcagaaga attatgcagt
      601 tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt tcacaacatt
      661 gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac acaacatacc
      721 aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg atacaatgag
      781 cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat caagtcactg
      841 agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat gtgttctttt
      901 tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa attcaaatct
      961 aggtggggat atagagttaa aaatatattg acatgtgtaa atttacatga tatattcatt
     1021 tctgttgttt taagtagcaa agcaataaaa tagtatttta aaaaacatat aaaatagttg
     1081 ttgacatgtt agaaatgaca tgataagata actgagttgt taaaaaagaa ccacttgtga
     1141 agcttgattc tatcgaaaaa aacttcaaaa aaagttcttg acaaataaca aatgataagt
     1201 tatgataaat gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag
     1261 agttgacaat tacttgtctc tctgatatga tataaaagtt gctacggcag ccaaaaacga
     1321 atcggggaac actcctgatg aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa
     1381 atgtgtaggg cgtcttgatt caattcaaga caacaaacat tttttaacaa gcaagcaata
     1441 tgctagcaaa caaattgagc ttaacaatcg
//
LOCUS       DAJSVP010000193         1460 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSVP010000193 DAJSVP010000000
VERSION     DAJSVP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.200"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            106..612
                     /locus_tag="ON449_002749"
                     /pseudo
     CDS             106..612
                     /locus_tag="ON449_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            605..955
                     /locus_tag="ON449_002750"
     CDS             605..955
                     /locus_tag="ON449_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935261.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            952..1131
                     /locus_tag="ON449_002751"
     CDS             952..1131
                     /locus_tag="ON449_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935262.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            1143..1436
                     /locus_tag="ON449_002752"
     CDS             1143..1436
                     /locus_tag="ON449_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3935263.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT          529 a          230 c          283 g          418 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accgtaattc acaagtgtta
      121 ccacgagatt atactagaag aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa
      181 gtagccattc gtatcaggca gcataattgt aaaacaggat gtgttcattt aaacattggt
      241 acgtcgatct tagaaacgag accaggattt tcacaccaga tgaaaatacc tatcacagat
      301 aatacaaagg aattgcagaa ttattgtctt ttcttgtttg ataagtatta tgaaggacaa
      361 gaagtaaggc acgttggaat aacctatagc aagcttgtgt acaccgattc gcttcagctg
      421 gatttattta gtgatccaca aaagcaaatt gacgaagaaa atttagataa aattattgac
      481 aagattcggc aaaaatatgg ttttacgtcg attgtacatg cttcttcaat gttagaaggc
      541 gctagaagca ttactagaag cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt
      601 aaaaatgatt gattacaatg atcgtggacg tttaaagtgg gcagggtttt atttatctga
      661 tcatactgaa gaaattgatg ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga
      721 acaaatgacg actgaagaga ttgcggaagt attgaacgct gcagtcataa aaaatagatc
      781 agtatctatt caaaaagaag aacgaaacgc tgaaggatac tatccagaag atatcattgg
      841 caagatctta ggtagtgatg agctggggat ctacgttgga aaagacaaaa ttcactacga
      901 cgaaatacgt catatcgaat tttaccagga taataaatgg agtcgattag aatgaacatt
      961 atccagcaat atgagctaaa gtatataacc tttgatcagc tatcagaaga gatttggggt
     1021 tatggccaac ttatctctat gaatcctttt ttgtttttac aaataggagt agatactaga
     1081 ataatgcgtg tgttaaacta ttattgcaaa cgcaataata aaagaaagta ggcttacgca
     1141 ccatgtctat caatacattt gtttactcgc acccgatcaa tgtttacatt atcaagaatt
     1201 taggaatcac tgtagaacaa ttttgtgagt tatatgccta cccacaagga acggttgcaa
     1261 gctggataac ccgacaacgt agaataaaat cattacctgc ttcatttgta tatgatctaa
     1321 gtcttgcttc tagtatgaat atgaccgatg tatacgaaaa gttattaata ctagaaaatg
     1381 aatatgatca attcaattca aatcagcagc gaaaagtaaa gaaacagata gactagaata
     1441 gctattttcg aaaagcaata
//
LOCUS       DAJSVP010000194         1421 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSVP010000194 DAJSVP010000000
VERSION     DAJSVP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.201"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(73..480)
                     /locus_tag="ON449_002753"
     CDS             complement(73..480)
                     /locus_tag="ON449_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935264.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(499..1317)
                     /locus_tag="ON449_002754"
     CDS             complement(499..1317)
                     /locus_tag="ON449_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3935265.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          420 a          235 c          196 g          570 t
ORIGIN      
        1 tgtataactt atactttaat attcatttat ctagtataac ttatacttta attgaatacc
       61 gcatgaaaaa aattattttg gtaatttttc aaactcttct tgtatttctt ctcttatgtg
      121 ttcttctttt gctactccta gtctatgctg gatatatgag tctgctaatg tattaacaat
      181 tttttcgaaa gaaattctcc ctttttcact ttgtaactcc gtcatatagt cttgtaaaat
      241 tgagagtttg ctatttaatg ccggagagat ctttttagga actgatacct tgtttctttt
      301 atcaggaatt gaaactggtc ggcctctttt tttcaatgta atatttcttg tgttactcaa
      361 tgcactattg ctaaagttat attcaccgct tattttcaca tcttggcgtt tgttattggg
      421 taataatggc tcaaattcac cattctccat cattttagga atatcaaaat ctttaggcat
      481 tacttatgca actccttatt atttatttaa aaactcttgt ctagcatcta gttctgccaa
      541 aatatttata aatacttggt gtgctttata atcccattga tcaaaatttc catttttgta
      601 ttttcttaaa tgtataccaa ttttagaata ccttctcaat cttttttgat aatgaatgag
      661 atttttggat accacggttc cgtatttttc aattgcttct tctaggattt cttcatcaaa
      721 atcatcattt ggttctacca tacaaggtac aatatctata atttgcaaat caagatcgta
      781 tctatcgacc atgaattttt gataatttac atatttgttg actccctcta aggattcttc
      841 agctgtttga aatgaaatta tcgaatagtc tgaagctgcc atagcattat cggaaaattc
      901 gctaatggtt ggtggtacat caataaatat ttcttgataa ttgttcttta ttggaagaag
      961 taattttttc aagacagtaa cttggtctaa ttcattttca tgtttattag caaaagcgga
     1021 gaatgatcga aaactagtgt cacatgccag aaggtcaaga ttttcattta ccttaataat
     1081 actacttgct aattctccat ttgcgacacc atttgttata gtggttttga tatcggttat
     1141 accgaaagtt cttgctatga cttgtgttaa gttcgcttga gggtctaaat caataataag
     1201 cgtttttcta tttcttatca ttgcattgtc ataagcaagc atgctgacta tcttagtttt
     1261 acctactcct cctttaaaat tgccaactgt aaatgtagtc ccttgaaaag tatccatgta
     1321 aatcctccca atctttaatt gataattaaa gtataactta tatatcggaa atggtcaaca
     1381 ttaaaagtat aacttaaata ataatatcta aaaacgtttg g
//
LOCUS       DAJSVP010000195         1420 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSVP010000195 DAJSVP010000000
VERSION     DAJSVP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.202"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(942..1355)
                     /locus_tag="ON449_002755"
     CDS             complement(942..1355)
                     /locus_tag="ON449_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR3935266.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          520 a          236 c          209 g          455 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa
//
LOCUS       DAJSVP010000196         1375 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSVP010000196 DAJSVP010000000
VERSION     DAJSVP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.203"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            191..445
                     /locus_tag="ON449_002756"
     CDS             191..445
                     /locus_tag="ON449_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3935267.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ON449_002757"
     CDS             446..763
                     /locus_tag="ON449_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3935268.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DAJSVP010000197         1364 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSVP010000197 DAJSVP010000000
VERSION     DAJSVP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.204"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>1364
                     /locus_tag="ON449_002758"
     CDS             82..>1364
                     /locus_tag="ON449_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3935269.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAJSVP010000198         1360 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSVP010000198 DAJSVP010000000
VERSION     DAJSVP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.205"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            53..1012
                     /locus_tag="ON449_002759"
     CDS             53..1012
                     /locus_tag="ON449_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3935270.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1145..1351
                     /locus_tag="ON449_002760"
     CDS             1145..1351
                     /locus_tag="ON449_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3935271.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          474 a          217 c          271 g          398 t
ORIGIN      
        1 tgtgctacac tcaaaatagt tccgaccaaa gaattataag gagtgagtac aaatgaccta
       61 tacccatctt acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc
      121 tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct
      181 gttcttcaag caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc
      241 aaactgtggt agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa
      301 ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg
      361 ttctgctcgt accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc
      421 gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg
      481 ccgctctatt catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct
      541 tgaaggtgac actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg
      601 attatcaaaa gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa
      661 aaaattaaat caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga
      721 ttgtggaaag gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta
      781 tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt
      841 acgtagagat ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca
      901 atctgtcgca tctaaacgaa ataatattcc tagaaaatca ctggattatc gaacaccttt
      961 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     1021 aaagataaaa ttgaacaact ggacgggcaa attgatgcct tgctttcaga aagagaaata
     1081 ttgatagaag aaaacaagcg attgaaaaag aaagtagccc aaccaatgaa gggaggtgga
     1141 tagtatgaac aatagaacct ttgatatttt gaaatggatc gctatagtag ttatccccgc
     1201 tttagcgact tttgtcggtc ttgtcggaaa aggtgttaac tggcggtaca cagatataac
     1261 ggtgatgatt attacaggtt ttggagcatt tctagggagt attttaggtg tatcaaatcg
     1321 aacgtataag aaaatttcat cagataaata ggaggataga
//
LOCUS       DAJSVP010000199         1334 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSVP010000199 DAJSVP010000000
VERSION     DAJSVP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.206"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            86..1165
                     /locus_tag="ON449_002761"
     CDS             86..1165
                     /locus_tag="ON449_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3935272.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAJSVP010000200         1323 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSVP010000200 DAJSVP010000000
VERSION     DAJSVP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.207"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            206..615
                     /gene="tnpA"
                     /locus_tag="ON449_002762"
                     /pseudo
     CDS             206..615
                     /gene="tnpA"
                     /locus_tag="ON449_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          512 a          185 c          228 g          398 t
ORIGIN      
        1 agctaataac taaaaaatgg ttcaatacaa atattttata aaaaatggta gtgcctatcc
       61 tttcagattg tagacaaaga aaaatttctt tgtctacaat ttttttgtta atgacttcag
      121 tcagtttcgt gcgcaaagcg tgcgaaacaa gaaaaccatg cgtttctaca cgtggaagaa
      181 ctagcatatc gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag cccatactcg
      241 ttggaatttt aagtatcata ttgtatttac accaaaatat cgacgaaaag taatctatag
      301 gcaattacga aaagatattg gaaaaatact taggaagttg tgcaaaataa aaaacgtgga
      361 aattatagaa gcacatgcaa cgccagatca tattcacatg cttgttcaag taccatcaaa
      421 aataagtata tccagtttta tagttatctg aaagatcgta gtgctgtgct aatccacgag
      481 cagcacgtaa atcacaaata tagatacgga aataaatcat tttggtcgga ggaatattat
      541 gtgagtacag tcgaattgaa tcaaaagaca attgaaaaat acatccgtga acaagaagct
      601 gatgatcgca tttgagatag tataagtaaa agagagtaga tgttgcaata acaaaagggt
      661 aaaaatggac tacctccagt aaaataccga aaaatagttt aacttttgga gataagtcca
      721 tttaagggtc tatagaatga caattttttt aggggatttt actattatta cttcaagacc
      781 cgcttggatt cgcaaagtga gtgaactctt aaaactacct gatagtaggt tgttttaaga
      841 gttatctgaa agtaaaaatt gcatttatat ttttgataaa tacgtaaatt tatatatacg
      901 tatttatcaa agttttaggc caaaggaata ttgtgtaaat acactaggat tgaatgaata
      961 gaccagaaga aattgcgtcc aagaacaaaa gaagcatgat agtgctctat ataaattggg
     1021 cattcaagaa tctgaaattt aaaggtagaa ttttagttag acttttagat tttaaacaca
     1081 aaaataacca tacaagcaac aagaagcaaa gtgctatatt ataaattgtc cgtccgttta
     1141 cttacataag atcattgggg ctatttttaa aagagaataa tagataataa atgaaaaaaa
     1201 agtcaagtcc acaatcctgt gtaaaatact atacaatgct ctcactattt tttacttggc
     1261 aaatttaata tattttcttg atgaagaaag ccactcatcg tgttgatcaa taaggactgc
     1321 tcc
//
LOCUS       DAJSVP010000201         1311 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSVP010000201 DAJSVP010000000
VERSION     DAJSVP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.208"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            200..742
                     /locus_tag="ON449_002763"
     CDS             200..742
                     /locus_tag="ON449_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935273.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="ON449_002764"
                     /pseudo
     CDS             898..1248
                     /locus_tag="ON449_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAJSVP010000202         1269 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSVP010000202 DAJSVP010000000
VERSION     DAJSVP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.209"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            96..1181
                     /gene="xerS"
                     /locus_tag="ON449_002765"
     CDS             96..1181
                     /gene="xerS"
                     /locus_tag="ON449_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3935274.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          507 a          214 c          193 g          355 t
ORIGIN      
        1 atttcagagt ataccaattg acaaaacact ctataattgg tatagtccaa ttatagagat
       61 actcctctcc cctacttatc gaaaggatga ataacatgaa aaatacagaa atatatcaaa
      121 aaatagatac aatcatactt catatgcccg attatataaa agattttgtt caagatcgtg
      181 aggataaaga tcaatcacct cgaactactt tagaatatct aaaaaattat aagctctttt
      241 atgaatggct tctgagtgag tctatcgttc ccgatacttc aatcacctcg ataagacagt
      301 taactgccta tgatctaaac ttgtacaaat ctcatttgaa acgtcgcaca aaggaaaatg
      361 taaaaaaaga gacaacaaaa actaagctca aagataataa taacttaggt ctatctactt
      421 ctactatcaa tagaaatatc acagcattaa aggttctatt taaatatctt tcgaaaagtt
      481 ccaataatcc tttaggaaaa ccatacttag aaaataatcc tatggatcaa gtagcaacta
      541 tcacagataa aactacgtta gctgcccgtg caaacgcaat cgagaaaaaa ttatttctag
      601 acgaagatac acaaaattat ctagattata ttgccaatga atataaaaaa actttgagca
      661 aacgagcttt gatctattat caacgagatg tagaaagaga cctggcgatc aatgcattga
      721 ttttaggatc tggattaaga ctctccgagg ttgtaaatat taatttagag gacctatctt
      781 tagaaaagaa taatgtggta gtaactagaa aaggaaataa gagagacgca gtaaatatag
      841 cagcatttgc aatggaatat ttagctaatt atctagctat tcggaaagaa cgatacaaag
      901 taacagaaaa cgaaaaagct ttatttctag caatttatca aggagaagcc aagcgtatat
      961 ccggtattgc aatcgaacgg atggtagcaa aatattcaaa aggattcaga gtccaagtca
     1021 gtccacataa attacgtcat actttggcta ctagactcta tcaacagaca aattcattgg
     1081 tattaactgc ccaacaattg ggacatacaa gtacaaatac aacaacttta tatactcata
     1141 tagacaatgc agctacaatt gatgctttga acgctttgta aatgtagtaa aaaatagtat
     1201 aataatgaag cctgtctcta aaattagata aaagcccaac tttaaaggca agcttcatta
     1261 tcattttta
//
LOCUS       DAJSVP010000203         1240 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSVP010000203 DAJSVP010000000
VERSION     DAJSVP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.210"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1240
                     /locus_tag="ON449_002766"
     rRNA            <1..>1240
                     /locus_tag="ON449_002766"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          325 a          279 c          383 g          253 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg
//
LOCUS       DAJSVP010000204         1141 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSVP010000204 DAJSVP010000000
VERSION     DAJSVP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.212"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..579
                     /locus_tag="ON449_002767"
     CDS             25..579
                     /locus_tag="ON449_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3935275.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            833..>1141
                     /locus_tag="ON449_002768"
     CDS             833..>1141
                     /locus_tag="ON449_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935276.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          465 a          115 c          211 g          350 t
ORIGIN      
        1 atatttttaa gaagggaaac aatagtgagc tataatgtac aaccgttaag gacaaatgaa
       61 gagatacaag attttcagtt ttggctaaga agaacaagta atccagaaag agatagtttt
      121 ttatttttgt ttggaatcaa taatgggtta cgtatgtcag atattatagg attaaaagtt
      181 ggagacatac gaggtaaaag taagcctatt attgttgaac ataaaacagg taagcgaaaa
      241 ccgattttta tagataattt gagagaagag attttattat atactgaagg taaagaagaa
      301 aatgattggt tatttccaag tcggcaacaa ggtcgacata taacaaggga tcgtgtctat
      361 cagatatatg cagatatagc agaaaaatta ggaagagatg atataggaac acatacatta
      421 agaaaaactt ttgggtatca ctactacaag aaaactagag acattgctac cttaatgttt
      481 atttttaatc atagctcgca agcaattaca aaaaggtaca ttggtataac tgaagatgaa
      541 ataggggcat cgttacgtgg ttttaaattg ggaggctaat tgagatatta ggatatattt
      601 tttgtgtata atgaaaactt agaaggtgag attaatgaag ataactgaat tttcggaagt
      661 atttaaaacg tttagtaaca tttacaatag aataccagag atagaacaaa atgttgtagt
      721 tgataatgat tattatatag agaatgattt taataaacca agaaaatatg acaacctata
      781 caattttata cacaatagaa caacaataaa taacaacagt aacactcgag ttttgattaa
      841 ttcagcatat tttcatatta aatatttaaa gaaatatgag tatgaagaag ttgtattaga
      901 ttatgggcta aatagacagt catttttaat ttatgcaata aataacggaa atttgccaag
      961 caataaaaag aacataatta ttgaaacata tgcctatctg gaggggggat ctaaaaaact
     1021 aattaagtca tgtaatatag agtatcaatt atcagtagga gaaattaaat gtgttttcga
     1081 agaagattta gaacttgact taaaaaaaat ttttgccagc attcatgatt atataggttt
     1141 g
//
LOCUS       DAJSVP010000205         1140 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSVP010000205 DAJSVP010000000
VERSION     DAJSVP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.213"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..749
                     /locus_tag="ON449_002769"
     CDS             <1..749
                     /locus_tag="ON449_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR3935277.1"
                     /translation="TENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIR
                     NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALI
                     QENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKE
                     IANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDS
                     SHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            766..>1140
                     /locus_tag="ON449_002770"
     CDS             766..>1140
                     /locus_tag="ON449_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935278.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTF"
BASE COUNT          427 a          125 c          200 g          388 t
ORIGIN      
        1 atacagaaaa tctaagtaaa actatcgagc aaatcaaaat agataatctg ttatcacaaa
       61 attacgatcc agaaatatat ctgtttcttt atgaaaataa aatactggaa tatgttaaaa
      121 atggagatat tcgaaattta gaaaacatgg tttttaatct gagtaatgga ataattccaa
      181 gtgttagtgg agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt
      241 tagcacaaac gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg
      301 ctttgataca agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt
      361 ctgggattgt ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct
      421 taatttcttc tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg
      481 aaatagctaa ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa
      541 tgcatattac gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt
      601 tgaaatcaaa ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac
      661 acttttcgag agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt
      721 tagtggacaa tttcaacaac attagctgag aggttttatt ttattatgag actatatcat
      781 ttacgtagtt tggaggagtt gaggaaattg gattttaaca aaaaattttt ttgggaaatt
      841 tctgatttga gttgtttcgt agaatctgag cgtctaagta atggtaataa tagtgtgtta
      901 aatgaatatg acaaagtagt aattaataaa gtaaaaagtt tatgtgaaga agctagagaa
      961 tctatcagtc atattaatgg tagcaatttt aaacaaacat tatttaatct gattaagcta
     1021 gatgctaaaa tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa
     1081 aaattggatc agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt
//
LOCUS       DAJSVP010000206         1136 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSVP010000206 DAJSVP010000000
VERSION     DAJSVP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.214"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..430)
                     /locus_tag="ON449_002771"
     CDS             complement(<1..430)
                     /locus_tag="ON449_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3935279.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            complement(500..1075)
                     /locus_tag="ON449_002772"
     CDS             complement(500..1075)
                     /locus_tag="ON449_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3935280.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          327 a          223 c          184 g          402 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcaaagaggg gggaattttt tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa
      541 aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt
      601 atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca acatttccta
      661 aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt actctagcta
      721 gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa ttagcaatgt
      781 gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca acgtgggcgt
      841 aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga tcaattactg
      901 gtttatcctc cgaagtagtt ggattatcat ccgaagtagt tggattatca tccgtaaaac
      961 catgagccaa atcataggct agtttttctt tacttacacc catttcagaa agataaccgc
     1021 aaggatctgt atgatcgccc caaatatttt gtgttaccca taaatgcgat ttgattcctg
     1081 tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga agaaaa
//
LOCUS       DAJSVP010000207         1104 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSVP010000207 DAJSVP010000000
VERSION     DAJSVP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.215"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            71..1018
                     /locus_tag="ON449_002773"
     CDS             71..1018
                     /locus_tag="ON449_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3935281.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVAIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          353 a          171 c          235 g          345 t
ORIGIN      
        1 tttcctaaat ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg
       61 aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt
      121 agccaatcat ttaggagaag aacatgtggc aatcgtcgtc gatgacttat caatgggaaa
      181 agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa
      241 atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc
      301 agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga
      361 aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt
      421 gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc
      481 cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct
      541 agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg
      601 tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta
      661 taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc
      721 tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga
      781 agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt
      841 gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg
      901 ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca
      961 accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa
     1021 catactaaga taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt
     1081 ttgtaaaatc aaatctggga taga
//
LOCUS       DAJSVP010000208         1094 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSVP010000208 DAJSVP010000000
VERSION     DAJSVP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.216"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..975
                     /locus_tag="ON449_002774"
     CDS             <1..975
                     /locus_tag="ON449_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3935282.1"
                     /translation="ENPFRVKQFSRMFDENKTDRNDALRIADFLRIQRFTTSPIKEEK
                     YMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLISLMTE
                     DFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVV
                     LSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIER
                     FEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKA
                     FYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          388 a          213 c          192 g          301 t
ORIGIN      
        1 gaaaatcctt ttcgggtgaa acaatttagt cgcatgtttg atgagaataa aactgatcga
       61 aacgatgcat taagaatcgc tgatttcttg cgtattcaac gattcacaac ttcccctatc
      121 aaagaagaga aatacatggc cttacagcgt ttaacacgta ctcgctatca acttattaag
      181 caattgattc gtacaaagca acattttctt gaaaatctaa cgtacaaatg taatacgctt
      241 gctcgagaaa tgcgagacga atcgacgagt ctttttagtg caaccctcat ttcactcatg
      301 acggaagatt tcacattgga tgaacttgct gagcttcctt tagaagcttt ctgtgattta
      361 cttcaagaaa aaggaaaagg ccgtttcaag caaccagaaa aaatcgctaa agcaattcaa
      421 cgtgcaatta ccatgagtta ccgcctaggt gctcttgcac aagaatcaat taatgttgtt
      481 ttaagtgttc tcgtacgaga aatacgagca cttgaaaaaa atatcaaaga attagataaa
      541 gccatcgaac aagtaattgt tgtcattcca gaatatcaat gcttaaccag tattccaggc
      601 gttggtaaag tctatgctgc cggtttaatc gctgaaattg gtcaaatcga aagatttgaa
      661 gatcagacaa aattggcaaa atacgctgga ttaagttgga aagtgaacca atctggaaac
      721 tatcagtcgc aaaatacacc tctgacaaaa caaggaaatc gatattttag atactactta
      781 gttgaagctg ccaactctgt aaaaaattat cttcctgaat acaaagcttt ttatcaaagt
      841 aaatataagg aggttccaaa gcatcaacac aaacgtgcac tcgtcctaac cgcaagaaaa
      901 tttacgcgtc tggtggatac gctactacgt aaacaccaac tctatacgcc accaaggagt
      961 gtgatagaga aataacaatc cgttattgac gcaaatcctt gaatcaaccc gaaaaaaatt
     1021 tgattttgat cgggtctagt ttcgtgcgct tcaaaacata ttactcaata aagaaaatct
     1081 taaatttcat cttg
//
LOCUS       DAJSVP010000209         1063 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSVP010000209 DAJSVP010000000
VERSION     DAJSVP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.217"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..993)
                     /locus_tag="ON449_002775"
     CDS             complement(37..993)
                     /locus_tag="ON449_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935283.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aac
//
LOCUS       DAJSVP010000210         1059 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSVP010000210 DAJSVP010000000
VERSION     DAJSVP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.218"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..398
                     /locus_tag="ON449_002776"
     CDS             6..398
                     /locus_tag="ON449_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3935284.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>1059
                     /gene="aac(6')-Ie"
                     /locus_tag="ON449_002777"
     CDS             399..>1059
                     /gene="aac(6')-Ie"
                     /locus_tag="ON449_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCR3935285.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VN"
BASE COUNT          424 a           82 c          191 g          362 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaata
//
LOCUS       DAJSVP010000211         1035 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSVP010000211 DAJSVP010000000
VERSION     DAJSVP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.219"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>1035)
                     /locus_tag="ON449_002778"
     rRNA            complement(<1..>1035)
                     /locus_tag="ON449_002778"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAJSVP010000212         1016 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSVP010000212 DAJSVP010000000
VERSION     DAJSVP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.220"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..>1016
                     /locus_tag="ON449_002779"
     CDS             33..>1016
                     /locus_tag="ON449_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3935286.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIY"
BASE COUNT          367 a          193 c          173 g          283 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      601 ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc
      661 tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct
      721 taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt
      781 gtatccatgt atctcgtaac atcgctcata aagtacgtgt caaagaccga aaagaaatct
      841 gtgatgactt taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat
      901 ccggcatgat tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa
      961 accaagactt attaactttt tatgattttc cacctagcat tcgtagaacc atttac
//
LOCUS       DAJSVP010000213          994 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSVP010000213 DAJSVP010000000
VERSION     DAJSVP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.221"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..264)
                     /locus_tag="ON449_002780"
     CDS             complement(<1..264)
                     /locus_tag="ON449_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935287.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSV"
     gene            complement(266..>994)
                     /locus_tag="ON449_002781"
     CDS             complement(266..>994)
                     /locus_tag="ON449_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3935288.1"
                     /translation="CSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSF
                     GTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWK
                     AALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVS
                     SGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNK
                     RDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          380 a          170 c          119 g          325 t
ORIGIN      
        1 aacggataaa tcatccgaga aaaattgatt atggttagtt aacaagttaa ttttacgttt
       61 aaaggaagaa ccgattttcc ggtgattcac aaaaacaaca tcattaaata gccctaattc
      121 ttttatacgc tcactatatc ttctagcgtt ttcaaattca gaataaataa ataaatctcc
      181 ttttgattct cggtaaaaat tttgataaac atttaatata ttaatcaaat gtaaaggagt
      241 ccaagcaatg aaagctgtct tcatattaat tctccttttt taaatacaag taagaaatgc
      301 ttcccaaata ataccagaaa acaattgaaa tgacatcgcg cttattgaaa agtacatgag
      361 cctccaccat ttcatctata aaaatagata gtataaaaat taaaacaata ccaagaaacg
      421 cataattcac tttacttcta aaaaatgttt tgagtaaact aaagatattt aacacataga
      481 aagcatagat acatataaaa gcaattatac cacttgatac aagtacttga agaaatccat
      541 tatgcatgtt tccatgtgct ctttttagtt cattcacatc tgtagtatca agatctactt
      601 ttttcaaaaa ttttgcatcg ctattcccat agaaatcggt ctctccataa ccaaataaca
      661 aagttggtct tataacttta agcgcagctt tccaaatcgt aaaacgacca gctgaaattt
      721 cggagttatc actagaatgc tgtaatatta cttctttttc tggaagtgga gttttatctg
      781 attcatttga ctctataact tctacttttc tatcaatcga tacaggaatt gttgctaaaa
      841 aatccttagc ataattagta ccaaaagaaa aatataccaa tagaactaat aatatgctta
      901 ccatccattt gatttttaca ggtagtactt ctctaatgct ctttctaaat tttgaattgg
      961 caaacaacaa aagaaataat actaaaaaag aaca
//
LOCUS       DAJSVP010000214          984 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSVP010000214 DAJSVP010000000
VERSION     DAJSVP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.222"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            151..>984
                     /locus_tag="ON449_002782"
     CDS             151..>984
                     /locus_tag="ON449_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3935289.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          292 a          195 c          186 g          311 t
ORIGIN      
        1 cctcaacgac gtttagttta tgaaaacagg ctctatactt tttgatgttg gtgaacttca
       61 ccaacatctt ttttgtataa aaaaatatag aaaagacacc ctgaaatcct ttagaattaa
      121 tttggctacc aattcatagg aggattcaag atgtctcata ctcagcttat caaagatacc
      181 ctaaatattc tagacctaaa tatctatttc gaggaaaatt gtttaactaa agaaaaatac
      241 aaagggcaaa tttgcttgat ttacaaagga actcttctgt acaagccaga agagtgttcc
      301 cattgtcttt gtgtcgtccc ctctaggatc attcgttggg ggacgacaac cgttcggttg
      361 ttactgaatg atgtgtctga gtatcgcact tatctggagc tgaagaaaca acgatttaaa
      421 tgtaagtctt gccaaagaac attcgtagcg gatacctctg ttgctgaaaa acactgtttc
      481 attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac ttccatgacg
      541 gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat gaagcgattc
      601 taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt tgatgaattc
      661 aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg acaaagccat
      721 gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta tttttctcgg
      781 ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta tgcgccttat
      841 gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg tttccacatc
      901 gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac ggtacgactt
      961 aaaggaaatt cccactctca acga
//
LOCUS       DAJSVP010000215          979 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSVP010000215 DAJSVP010000000
VERSION     DAJSVP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.223"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            346..979
                     /locus_tag="ON449_002783"
                     /pseudo
     CDS             346..979
                     /locus_tag="ON449_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          181 c          178 g          288 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacct
//
LOCUS       DAJSVP010000216          964 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSVP010000216 DAJSVP010000000
VERSION     DAJSVP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.224"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(62..280)
                     /locus_tag="ON449_002784"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="ON449_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="ON449_002785"
     CDS             complement(546..740)
                     /locus_tag="ON449_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935290.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="ON449_002786"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ON449_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          328 a          166 c          150 g          320 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aaga
//
LOCUS       DAJSVP010000217          953 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSVP010000217 DAJSVP010000000
VERSION     DAJSVP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.225"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..>953
                     /gene="bsh"
                     /locus_tag="ON449_002787"
     CDS             6..>953
                     /gene="bsh"
                     /locus_tag="ON449_002787"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3935291.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIE"
BASE COUNT          330 a          126 c          172 g          325 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaa
//
LOCUS       DAJSVP010000218          943 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSVP010000218 DAJSVP010000000
VERSION     DAJSVP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.226"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>943
                     /locus_tag="ON449_002788"
     rRNA            <1..>943
                     /locus_tag="ON449_002788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAJSVP010000219          923 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSVP010000219 DAJSVP010000000
VERSION     DAJSVP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.227"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          364 a          128 c          147 g          284 t
ORIGIN      
        1 atacatgaat aggaaaggaa acagaaggag gcaatcttaa ttttttaaat tgtctaaatt
       61 atatagaaag aacaatcttt ctatataatt agacataaga gagactacgg ttgatttgat
      121 attaacaagc gtatatgaat gtatatacaa ataaaaaagg ggagggagaa gttaaagaaa
      181 attcagagaa gtggggcact cagtagtatt aacaatttaa aaggaatttc ttaaagaaaa
      241 tgggttgcat cctggagata caattttttt agataaagaa aaaattcaag caacaattca
      301 aaaaaatctt aactgctgag attgatgtag caatatctca atcatcaaca aagtacgatc
      361 ctgcctttca aaagcttatt gataaatgtt tattcattat aaaaataaaa gaaaaattta
      421 tgtcagcttt tttcatcaaa gtggtaatga taattcgtct attaattgta ttttttagga
      481 aatatggtat aattgtttta acgaaaaggt aattcatgat acaccaaaaa ccactagctg
      541 gcgggctagt ggttttcttt tacgttctat tcagattaac gggtaggctt actgtctttt
      601 ttggcaataa ttttgcaatc aagccattta cagatgtaat gcgcggatac ttctgctaag
      661 attgcaaaaa acatatcgaa aaggtaattc ataatacaac ctcctcccct tgccataata
      721 ggccggggta gcagacagca taaatattat accataactg aaaatttcat tttagtacat
      781 ttcaatatat tgatgaacca gcacaataaa acatgccatg actaacagaa aaaaacttat
      841 taaaccagat ataacttgta aacatccaac tatatagaaa acgtaaattt tctatatagt
      901 tggattaaga taacgactat tta
//
LOCUS       DAJSVP010000220          918 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSVP010000220 DAJSVP010000000
VERSION     DAJSVP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.228"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..132
                     /locus_tag="ON449_002789"
     CDS             <1..132
                     /locus_tag="ON449_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935292.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            284..814
                     /locus_tag="ON449_002790"
     CDS             284..814
                     /locus_tag="ON449_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935293.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(809..>918)
                     /locus_tag="ON449_002791"
     CDS             complement(809..>918)
                     /locus_tag="ON449_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935294.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          361 a          119 c          143 g          295 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 ctaaaatttc taacatcagt tattatgata attttgatga tgtcaattat gatttacagg
      241 aaagatataa tgatttaatc gaagaattag aggagagagg agaatgattt cagataataa
      301 atgggaaata aatctcaaaa gatttatttc agaatatata tatacgattg aaactaaatc
      361 tagtttttct cttaatgaag acagtgatcc tctaaaaaaa tacactattc aatgtaagaa
      421 aaataaagat gaaaaattgt tcgtaataag accagatctt atgaagagta atacccacta
      481 tataaaagaa aaacctaaag attgtgacta catcatcata agtgaagcaa ataaaacagt
      541 atttctgata gaacttaaaa gctcagctca gacttcccct gcaaaagaaa ttagtgaaca
      601 aattgatggt ggatataagt ggttacaaca tcttagtttt ctaacaaaat atgattttac
      661 tgaattcagt tataaaatta ttaaggtatg ttgtaaattt gaggcaagac aagatcgtaa
      721 aaatcttttt aaacctaatg aatatggtat ttataatatg aatggtccaa aattagactt
      781 aagagagttg tatagaaagt gtaacgatct ataaattcaa atctttttta attctatata
      841 ttgtttgtct agtaacgtta taattctttg caatctttga aatagagacg ccctcattca
      901 agtccttaac gatgtttt
//
LOCUS       DAJSVP010000221          888 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSVP010000221 DAJSVP010000000
VERSION     DAJSVP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.229"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            133..345
                     /locus_tag="ON449_002792"
     CDS             133..345
                     /locus_tag="ON449_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935295.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            507..>888
                     /locus_tag="ON449_002793"
     CDS             507..>888
                     /locus_tag="ON449_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3935296.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDF"
BASE COUNT          329 a          117 c          145 g          297 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cctttcattt atattatatc tcattctttt atataatgaa
      481 agtaaataaa tcatggagga taaaatatgg ataaatttaa tagattaaca ttaataaatc
      541 agtttgaaat tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag
      601 aaatactaac agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc
      661 cattgccaga agaagattcg cggtttgttc tagacatatt aaatatgtat agagatatta
      721 cttttagtaa aaataaatta aaagatataa attcaattga taattattat atacaattta
      781 aaggttttga ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta
      841 ttgaaaaatt aaataggttt caagaaatag ttgaagatag tgatttta
//
LOCUS       DAJSVP010000222          861 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSVP010000222 DAJSVP010000000
VERSION     DAJSVP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.230"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            58..729
                     /gene="radC"
                     /locus_tag="ON449_002794"
     CDS             58..729
                     /gene="radC"
                     /locus_tag="ON449_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3935297.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT          261 a          151 c          163 g          286 t
ORIGIN      
        1 taaaaatcca tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg
       61 ggaaaacgat tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt
      121 tatggcgcgg aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa
      181 cacccctata gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc
      241 tctttacgac aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa
      301 gcaatcgaaa tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga
      361 gcggctccac cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa
      421 gatcacaaac aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta
      481 aaaaaaacag tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc
      541 cattatgctg tacgttattg tgcagcaagg atcgttttag cgcataatca tccttccgga
      601 atatataagt tagtacacat aaattatata aggaaattta ctgttatatt gccatctgga
      661 aatactatta tgttatcgat aattgaacgc cataatgcat ttttttcttc ttccgaagat
      721 tctttataaa tttcatcgaa ttcttttgat agaataaatt cgacattttt tatatctatt
      781 ttagctggct ctacttcaat ttcatttaat tctttcatta aagcttctcg atcttttttg
      841 tattcctcta agtctataac t
//
LOCUS       DAJSVP010000223          860 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSVP010000223 DAJSVP010000000
VERSION     DAJSVP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.231"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(532..756)
                     /locus_tag="ON449_002795"
     CDS             complement(532..756)
                     /locus_tag="ON449_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935298.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          311 a          115 c          153 g          281 t
ORIGIN      
        1 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
       61 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
      121 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
      181 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
      241 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      301 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      361 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      421 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      481 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      541 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      601 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      661 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      721 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      781 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      841 aattataagg gataagtagg
//
LOCUS       DAJSVP010000224          821 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSVP010000224 DAJSVP010000000
VERSION     DAJSVP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.232"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            245..>821
                     /locus_tag="ON449_002796"
     CDS             245..>821
                     /locus_tag="ON449_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935299.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SELLSFGIGLTLLICLIILTFNFKKLGILSKI"
BASE COUNT          260 a          114 c          119 g          328 t
ORIGIN      
        1 ggaaatattt ggcttttatg atacggcatt tcacattaat cgtgcgttaa gtttagaatc
       61 tatttttagc tcccctatta attttgaaac gtttagatca tatggcatgc aagtaaataa
      121 tttttaccca tggttaactc tatatccttt atttttattg atcaagttta ctaatttagc
      181 aataggctat aacctttttc tttatattgt tacgctcatt actctattta tatgtcatta
      241 tgtaatgtat gaaattacca aaaaacatgt tactagctcc tttttcgcta ttatatatac
      301 aacatcttct tttaggagtg tagaaatatt tcttcgaggg gcaatggggg aattactcgc
      361 aatgtcaata cttcctctta ttttactcgg gtttataaag ctttacgact caaaaaaaga
      421 atcttgggtt atgttggcga ttagcatgac tttattaatt tacactcatg ttctttctgt
      481 agctatgact atggtaatga ttataattgc tttaattatt caattttatc ggaaaaagaa
      541 aatagaaatc ttcttaatta ttaaattaat aaaagctgct atagttactt tacttctttc
      601 actagcgttt ataggtccta tgattgagca gacattgtat caagatctga atagacctta
      661 tgtggatata ttacaaaaaa gagcaattta tctgggtaaa gagtttttga ttggaagtat
      721 agattcagaa ttactatcat ttggtattgg cttaactttg cttatttgtt taattatact
      781 aacatttaac tttaaaaaat taggaatctt atctaaaatc a
//
LOCUS       DAJSVP010000225          811 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSVP010000225 DAJSVP010000000
VERSION     DAJSVP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.233"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..672
                     /locus_tag="ON449_002797"
     CDS             <1..672
                     /locus_tag="ON449_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935300.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSVP010000226          742 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSVP010000226 DAJSVP010000000
VERSION     DAJSVP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.235"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..731
                     /locus_tag="ON449_002798"
     CDS             51..731
                     /locus_tag="ON449_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3935301.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          266 a          125 c          148 g          203 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat ac
//
LOCUS       DAJSVP010000227          740 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSVP010000227 DAJSVP010000000
VERSION     DAJSVP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.236"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..618)
                     /locus_tag="ON449_002799"
     CDS             complement(<1..618)
                     /locus_tag="ON449_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3935302.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISY"
BASE COUNT          242 a          147 c           82 g          269 t
ORIGIN      
        1 atatgaaata tggaatatga tatcttcaga tatataatcc ctttctgacg gaaacttgat
       61 attgtgctgt tgaattatct cagatgaata cagcgcatgc caaactgaca ttggcaatat
      121 aaaatcgact ttataactcg gctcagtgcc aatcattcca agcaacactt tttcaattat
      181 agcgttattt gtaaatatcg tactatcata tcttgctggc tttgaaataa tattattatc
      241 atcataatat tcataatatc cagcataaca tgtgtctgca ttactccttt ttataccatt
      301 taacattgct tcaatataat tagctgcaac aaaatcatca gaatcaataa aagcaacata
      361 ttttcctttt acatgttcaa gtcctgtatt tcttgccaat cccaagccag cattattttt
      421 atgaataata cgaatccttg aatcctttaa tgcatagtct tcacatattt tattagatga
      481 atccttagat ccatcatcca caattataat atctaggttc ttatatgttt gattacacac
      541 tgattccaaa caacttccta catacttttc aacattatat acaggtatga ttacactaat
      601 tagatcactc gaattcatcc ttctatcctt tcactcacgt tttctcaatt agtcaatgac
      661 ggcatcaacg attttcttaa attctctgaa aaaaatagac tctgatcttg atgatgtgaa
      721 tgacatacta tcttcttcta
//
LOCUS       DAJSVP010000228          711 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSVP010000228 DAJSVP010000000
VERSION     DAJSVP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.237"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..154
                     /locus_tag="ON449_002800"
     CDS             <1..154
                     /locus_tag="ON449_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3935303.1"
                     /translation="EENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVL
                     KIAEEK"
     gene            155..>711
                     /locus_tag="ON449_002801"
     CDS             155..>711
                     /locus_tag="ON449_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3935304.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASM"
BASE COUNT          275 a           87 c          131 g          218 t
ORIGIN      
        1 tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt
       61 gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc
      121 aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg
      181 tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct
      241 agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg
      301 cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc
      361 gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga
      421 agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg atttaattat
      481 agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat taatagatga
      541 acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc
      601 ctattttaat atggtaaaaa gaattgaaaa tgaagttact atagttaata aatcaagata
      661 cacatctagg caacagacac ctgaagtttt tgaaatgaat gcctcaatgt a
//
LOCUS       DAJSVP010000229          708 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSVP010000229 DAJSVP010000000
VERSION     DAJSVP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.238"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(198..>708)
                     /locus_tag="ON449_002802"
     CDS             complement(198..>708)
                     /locus_tag="ON449_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3935305.1"
                     /translation="NTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKE
                     CLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNI
                     EETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKE
                     ILKVGNIDE"
BASE COUNT          248 a          112 c           82 g          266 t
ORIGIN      
        1 aaaaacgcat tttaaaaaaa tttttcttta taaaaacctc atacaaaact ataattccac
       61 cccaagcaaa agctattttc atcgggatat tagttacatt aaaagtacta ggcataaaat
      121 aaagcagaca aatgattaaa tttattactc gaaacgcatc tcgacttaat aaactcttct
      181 ctaaaacagg tactattcta ttcatctata tttccaactt tcaaaatttc tttatcaatt
      241 ctatttaata gtttatcaga gtaatccgat ttaacttttc ttacaaataa tttttcactt
      301 tcttttattt gatctatatc cctttcatca aaccatttgt tcatggaagg atcaataata
      361 tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt caattcaagt
      421 ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa aaaaagtgta
      481 tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta gtcttgttga
      541 ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc ccaccattga
      601 gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt aatcatcctc
      661 agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgtta
//
LOCUS       DAJSVP010000230          689 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSVP010000230 DAJSVP010000000
VERSION     DAJSVP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.240"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>689
                     /locus_tag="ON449_002803"
     CDS             82..>689
                     /locus_tag="ON449_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR3935306.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAJSVP010000231          671 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSVP010000231 DAJSVP010000000
VERSION     DAJSVP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.241"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..447)
                     /locus_tag="ON449_002804"
     CDS             complement(<1..447)
                     /locus_tag="ON449_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935307.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          198 a          120 c           90 g          263 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagac
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt a
//
LOCUS       DAJSVP010000232          671 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSVP010000232 DAJSVP010000000
VERSION     DAJSVP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.242"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..662
                     /locus_tag="ON449_002805"
     CDS             <1..662
                     /locus_tag="ON449_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3935308.1"
                     /translation="KKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPD
                     WHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDS
                     FTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTD
                     IQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEV
                     N"
BASE COUNT          253 a           78 c          115 g          225 t
ORIGIN      
        1 ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga aaagaaattc
       61 aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac catgtaggac
      121 agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt tcaaacaaaa
      181 aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata gatacatttg
      241 ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat attgattatt
      301 cggacagttt cactcttatt attgagcatg aaactggtat agttggaact ttaagtgtag
      361 atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag attttaatag
      421 aatgggaagg taacccggat tctctatttt catggaatga agaaacttct agtatgacag
      481 atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact attattgaag
      541 atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa atggaacccc
      601 aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt gaggtaaatt
      661 aatgatgaaa g
//
LOCUS       DAJSVP010000233          651 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSVP010000233 DAJSVP010000000
VERSION     DAJSVP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.244"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..535)
                     /locus_tag="ON449_002806"
     CDS             complement(<1..535)
                     /locus_tag="ON449_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3935309.1"
                     /translation="MKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSE
                     ISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISN
                     IGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFR
                     KQEVNKINQRKEFFKVSL"
BASE COUNT          186 a          114 c           67 g          284 t
ORIGIN      
        1 ctagtgaaac tttgaaaaat tcttttcttt gattaatttt attaacttct tgttttctaa
       61 aatgattatg caatttagtt tccaaagacg gagcgtcctc tgaaaaaatc atcgcatgaa
      121 cgtcaaattc aaatggaacc gatgcactac tcaattcttt tattctatcc ataggttcta
      181 atcttctagt cataccaatt ttatatatgt cttcaccaaa agacccaata ttactaatta
      241 catacacata accggctcta gtatttaatt ctctttgtaa tacattttca cgtataactt
      301 ctaattcttt taatttttct tccagttctt gaattttatt tgcatataat tctttttcca
      361 catctgcatt agctttttgc atatatgcga ttaatttgga aatttcagaa ttgaattgac
      421 gttgttcttt atcaatacgt ttcttttcac gatcaatttc tctacgaact ttttcctctt
      481 ctatcatctg ttcttttatt gctttttgtt gttctttttc ttcggtaatt ttcactaaat
      541 aagagtaatg aagttctagt tcttgaagtt taaactctaa aagacccttc cttaattcag
      601 cattgtcatt cttgaataaa ttattaattt tatcaaatat ttttatgatt t
//
LOCUS       DAJSVP010000234          629 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSVP010000234 DAJSVP010000000
VERSION     DAJSVP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.245"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            58..546
                     /locus_tag="ON449_002807"
     CDS             58..546
                     /locus_tag="ON449_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3935310.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          256 a           51 c          113 g          209 t
ORIGIN      
        1 ttttatgtga aaacatgagc aatagaattg tactagtagg ataaggatgg atggaaaatg
       61 aaaaaaacta tttttgtttt agcgattttt atatgttttg ggctactttt ggtaggctac
      121 ttgttgttta attctaatga aatgagaact atttctacga atgacttgtt aaaaaaaagt
      181 gaattaaagg ataaaaaaaa agaatctctt gcctttataa tgtttactca agaagagtgt
      241 gagtattgta tagcaataaa tccaatcata gatagtgtaa ataaggaaac tttgaataag
      301 ataaagaaat atgatatatc tgtagatttt caatctaaac aaaagtgtaa aattaagttt
      361 gttccgacta ttgttgcata tagtaagaat aaagaagtgg ggagaattga caatgaatta
      421 gataatttgt atcgaaaaaa gcaagatggt tcaattgtaa tagacaagaa aaaaacaaag
      481 acattgctta tagattggat gaaaaaaatg gataaacaag gagaatattt gtatggagaa
      541 gattaaacag acatttccgt taattaataa atattggaat aagaaaaaaa tttttatatt
      601 ttttattacg tttctgctgt catctttat
//
LOCUS       DAJSVP010000235          624 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSVP010000235 DAJSVP010000000
VERSION     DAJSVP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.246"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>624
                     /locus_tag="ON449_002808"
     CDS             <1..>624
                     /locus_tag="ON449_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HCR3935311.1"
                     /translation="SNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAK
                     LLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANL
                     PNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSL
                     EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          222 a          111 c          107 g          184 t
ORIGIN      
        1 tcaaacttag cttacactat caagattatt cgatactttt tcatcaagaa gctcaccaaa
       61 ggtgaactag tcggaattat agatagtttt cctagtccat tatgtaaacc agttagaaat
      121 agacaagcaa aactattgaa tcaaatagcc aaagttggct ataactcaac aaaaaaatct
      181 tatttttatg gtcttaaaat tcatatgatc gttactaaga ctggctttcc gattacttac
      241 tcaatcacga atccaggtgt ccatgacgtg aaagtgttag agactttatc ggaagaagcg
      301 aatcttccca atatcctagg ggacaaagga tatattagcc ataaaatcca tgaaaagctt
      361 gcccttaagg gcattactat atccgttccg ccacgtaaaa acatggataa atcagaaaaa
      421 ctagaccaca gcttacttgg aaaacaaaga aaaacagttg agactgtttt ttcttcctta
      481 gaaaaattag gttgtcaaaa tttcaactca cgttctgtta aagggctaga gagtagattt
      541 gaaagcatat tactggctta cagtgttcta ttaagtcgag cacaacgacg ttttgaagga
      601 actttgagat attctttagg atat
//
LOCUS       DAJSVP010000236          612 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSVP010000236 DAJSVP010000000
VERSION     DAJSVP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.247"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>612
                     /locus_tag="ON449_002809"
     CDS             <1..>612
                     /locus_tag="ON449_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR3935312.1"
                     /translation="DMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNE
                     LRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGF
                     STSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA
                     MIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          214 a          123 c          113 g          162 t
ORIGIN      
        1 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
       61 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      121 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      181 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      241 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      301 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
      361 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
      421 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaagaaggca tcaccaacgc
      481 gatgatctat ccttacacca atggaaaaat agaagcgaag aatacccata taaagacaat
      541 gaaacgagta tcctacggat ttaaatcatt tgagaacatg agaattagaa tctttttgat
      601 caatcaatta at
//
LOCUS       DAJSVP010000237          569 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSVP010000237 DAJSVP010000000
VERSION     DAJSVP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.248"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(83..253)
                     /locus_tag="ON449_002810"
     CDS             complement(83..253)
                     /locus_tag="ON449_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935313.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            375..>569
                     /locus_tag="ON449_002811"
     CDS             375..>569
                     /locus_tag="ON449_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935314.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          233 a           85 c           71 g          180 t
ORIGIN      
        1 ataagaataa aaatggttat aaaaacaaaa tacccgaact attagaagct atttatacat
       61 aaaaacataa attcaacact atttagtgat gaaaaaatat tttcgcttca atatagttcg
      121 gaatagttcg gatattttca attttatgaa tcatttcaat ttcttcataa ataagtgcag
      181 tttcttcttt ctctagcttt tgccattcat ctttcaaaat ataaataaaa tcaaatactt
      241 ttttattttt caatttgctg attccacaaa aaagataaag tccttataat tgtgtaaaag
      301 taaaaaggcc atgtaacagt ccttttacgg tacaatgttt ttaacgacaa aaacataccc
      361 aggaggactt ttacatgacc caagtacatt ttacactgaa aagcgaagag attcaaagca
      421 ttattgaata ttctgtaaag gatgacgttt ctaaaaatat tttaacaacg gtatttaatc
      481 aactaatgga aaatcaacga acagaatata ttcaagcaaa agaatatgaa cgaacagaaa
      541 accgacaaag tcaacgaaat ggctattat
//
LOCUS       DAJSVP010000238          520 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSVP010000238 DAJSVP010000000
VERSION     DAJSVP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.249"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            187..520
                     /locus_tag="ON449_002812"
                     /pseudo
     CDS             187..520
                     /locus_tag="ON449_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          192 a          104 c           99 g          125 t
ORIGIN      
        1 gctacctttt taggtagcct actttaatct ttataccttt ctggatcaac gaaagtatac
       61 tttatatagt cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt catgaatcgg
      121 ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca cactggagga
      181 tttttcatgt caagacgtca acgaagaacc tattcaaaag aattcaaaca acaaatcgtc
      241 gatctctatc tcgctggtaa gcctcgcgca gaaattattc gagagtatga gcttacgcct
      301 tcttctttcg ataaatggat gaagcaagca caatcaacgg gctcattcaa agaaagagac
      361 aacttaacac cagaacaagc agaattgatc gcactaagaa agaaaaataa gcaactcgaa
      421 atggagaatg atattttaaa gcaagcggcg ctgatattcg gacgaaaaga caagtaattg
      481 atgccaacaa gcataaatat tccatatcag cgatgtgcaa
//
LOCUS       DAJSVP010000239          517 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSVP010000239 DAJSVP010000000
VERSION     DAJSVP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.250"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          225 a           53 c           82 g          157 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAJSVP010000240          508 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSVP010000240 DAJSVP010000000
VERSION     DAJSVP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.251"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAJSVP010000241          508 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSVP010000241 DAJSVP010000000
VERSION     DAJSVP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.252"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          195 a           59 c           68 g          186 t
ORIGIN      
        1 tatactacca aaaaatggta gtatattatt aatttatata atatgaaact taaaatatag
       61 tagtatgtta atttatacta aattaaataa agtactacct ttaataggta gtactttatt
      121 atcaagccat tattccttct tctactagtt ctttttgtca cataattttg caatcaataa
      181 atgttgcgca ataagtataa gaattccatt ttctaaattt gaaaggtgat ccataatttc
      241 atgttcaagt agtttattaa ttgtccactt gtcgcttcat ctaaaaattt tataggtttt
      301 aggaatgcta ttcgcttatt ttaggaagat tttaactgtt tgctatagat atatatcata
      361 ctagtatcgt aataaaagta ctctaaaaga agaattttca aatacgcaaa attaagtacg
      421 aaagagttga aacaaatttg gagaaaatga gcatagaatg tctgatgagt taaatagacc
      481 taaaaattat ctaaaagtgg agcaaaat
//
LOCUS       DAJSVP010000242          503 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSVP010000242 DAJSVP010000000
VERSION     DAJSVP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.253"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(331..>503)
                     /locus_tag="ON449_002813"
     CDS             complement(331..>503)
                     /locus_tag="ON449_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3935315.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          173 a           71 c           72 g          187 t
ORIGIN      
        1 aatttttact attcttacct tagcatattc tttataggta tatcagagct aatcaaacta
       61 aaaaataccc gaagtcttct tatgtaaaaa caccggttat tacttaaata tgacttaggt
      121 aaaacaatga gtgatccaat tataaaattt tatggcgaat aatttagtaa gcgctacata
      181 ttgaatgatt tagaaatttt gaaaaagtag ctagttcctt ttataccaga aaagtaaacc
      241 taataaaaaa cacaactaat ttttagctgt gttttttagg gagcgtcaat aattttgtgt
      301 aaataaattg tcctcctgca aaataattag ttactcagta aacattgaaa ctaatgtatc
      361 ggttacctgt tgaaaacctt tatggcttct gtttagaaat ttttgattgt atgtatcaaa
      421 aatgctgact agaaagcgtt ctagtgattc ttcattttga aactgctctt ttctacggct
      481 gtatctttta atttgcttat tga
//
LOCUS       DAJSVP010000243          496 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSVP010000243 DAJSVP010000000
VERSION     DAJSVP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.254"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(73..270)
                     /locus_tag="ON449_002814"
     CDS             complement(73..270)
                     /locus_tag="ON449_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3935316.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          161 a           90 c           72 g          173 t
ORIGIN      
        1 attacggtga ggaatttttt ctaccgacat aaacaaaaaa accacctcat atgaggcggt
       61 taacgttcaa cattatttta cgatatttac tgcttgagga ccacgttgtc cttcttcaat
      121 atcataagta actgcttggc cttcatctaa agacttaaag ccttctcctt ggattgctga
      181 gaaatgagcg aatacgtcat ttccatcttc accagtgatg aaaccaaaac ctttatctga
      241 gttaaaccat tttactgtac cgttattcat atatatttcc tcctgatacg tatataatac
      301 gtgttttatt gcaattaata ttacttggta aaaggagttt ttatagtgtg aatatatcgc
      361 tcagattacg tttcaaatta gattacttat ttactataac acggatgtaa tgctaacaca
      421 aggataatca ctcgatatac ggtttaaata ttattatttg tcaaattaag cgagacaaaa
      481 ctttccattt acgtaa
//
LOCUS       DAJSVP010000244          492 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSVP010000244 DAJSVP010000000
VERSION     DAJSVP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.255"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..252)
                     /locus_tag="ON449_002815"
     CDS             complement(22..252)
                     /locus_tag="ON449_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935317.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          164 a           68 c           64 g          196 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gt
//
LOCUS       DAJSVP010000245          486 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSVP010000245 DAJSVP010000000
VERSION     DAJSVP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.256"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..424
                     /locus_tag="ON449_002816"
     CDS             17..424
                     /locus_tag="ON449_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935318.1"
                     /translation="MFFLPLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
BASE COUNT          197 a           59 c           79 g          151 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc attaaaatat cttgaaattt actctgttaa
       61 aaaaaataaa ttaattggtg aaaatgattc tccaataaaa acacctattg agctaagctt
      121 aagaaatcgg atgggaatac tccaaagatc ggaaatagta aaagaacttt tttatcataa
      181 atttagttat gaaataagac tagatgatac ttatgagcat tatagaattg tttttttcgt
      241 atacaattct atagaagatg ctactgcgac tactgcatta acattcggtt ttacgaaaaa
      301 tggaacaatc aattcggata aaacccgtca agctgctaca gaaagtgatg atatatgtaa
      361 gaaagtatgc aatggggaag agaattgctg gataggggag gaaaagttga atgaaatcta
      421 ttaatgattt attaaaaaaa catcctgatt tatataaaaa attaaattct gaacaaagaa
      481 acctgt
//
LOCUS       DAJSVP010000246          481 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSVP010000246 DAJSVP010000000
VERSION     DAJSVP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.257"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          159 a           95 c           56 g          171 t
ORIGIN      
        1 aaatcactct acaataacgg cgaaagcaca aaaaataaaa aaattattac gttattaaaa
       61 aaacagatga aagatcaggt tttattagat ctcattcttt catcacttga agggatttaa
      121 tgaactactt tttcctcttt gtcgttttta tatattcgta ttccttcaaa cagtaggata
      181 gcctctttca atgcttgata tttgaactgt ccgttactta aaaatttaga tgtgccgccc
      241 tcttcctaga acatattctt ttttactttt tcattaattg acagaccaat cgaacgaaac
      301 tgtaaatttc gataggataa tgattggtca aactcattca ttttcaagac atctcgattc
      361 atattcaaga taggaatctt tttatcaaca tcactttatt cgtattttcc tctaccataa
      421 aagcttcacg ctccacaaaa ttgttttata cactactatt cagcccattc aacgaccaat
      481 g
//
LOCUS       DAJSVP010000247          481 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSVP010000247 DAJSVP010000000
VERSION     DAJSVP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.258"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            138..>481
                     /locus_tag="ON449_002817"
     CDS             138..>481
                     /locus_tag="ON449_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935319.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          214 a           65 c           70 g          132 t
ORIGIN      
        1 gtaacattta caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa
       61 gattaaatat ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt
      121 cgaaaggaat agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg
      181 aacgttatac ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca
      241 attcacgcca agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa
      301 taaaacgaaa tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa
      361 aatacaagcc aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa
      421 tttcaaaata tataaaagat gatgtacttg taaaaaacgt taatagtaat cttatacaag
      481 a
//
LOCUS       DAJSVP010000248          479 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSVP010000248 DAJSVP010000000
VERSION     DAJSVP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.259"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>479
                     /locus_tag="ON449_002818"
     CDS             <1..>479
                     /locus_tag="ON449_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3935320.1"
                     /translation="IFLIDVILLAKGTISITNFVIVFMYANMVPTLWTTLISIIWKWQ
                     DYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNISFSTEMNKL
                     FMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIATQIPYI"
BASE COUNT          198 a           57 c           67 g          157 t
ORIGIN      
        1 gaatatttct tattgatgtt attttattag ctaaaggaac aataagtatt actaattttg
       61 taatagtatt tatgtacgct aacatggtcc ctactttatg gactacttta attagtatta
      121 tttggaaatg gcaagactat tcagtgtcat taaatagata ttacgaatat tattcaaata
      181 acaacaaagt ggctggcaca aaaaaaataa ataaaataaa ttctattact ggtcaatcat
      241 taaatttttc atataacaaa agaaaggtat taagcaatat aagtttttca actgaaatga
      301 ataaactatt catgatatca ggagaaaatg gaacagggaa aacaacactg atgaaattat
      361 ttactaaaca gttgccggtt agtaataatc aattattcgt caacgggata gatattaata
      421 tgattgataa cgaatcatta agaaggttaa tttcaatcgc aacacaaata ccatatata
//
LOCUS       DAJSVP010000249          479 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSVP010000249 DAJSVP010000000
VERSION     DAJSVP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.260"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(100..399)
                     /locus_tag="ON449_002819"
     CDS             complement(100..399)
                     /locus_tag="ON449_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3935321.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          149 a           95 c           57 g          178 t
ORIGIN      
        1 ttagaataaa aaaatacaga ctctgttcaa ataaatgaac ctagcctgca ttatgagatt
       61 gactatactt cttaattcat tgagatgaat aactcgtttt tataaatgcc caaaagtaat
      121 cggtgccgaa gccattcctc gacctaattc tttttcaaat tttcctattg ttgatatctc
      181 catataaaac ggtgatactg gtttagacaa ttctcccctg attgctaacg gacgtaaact
      241 acgttccaaa ttatttatga ctttaggata atactgacct ttttctaagc ctgtctttgc
      301 gtctatcaaa agtatacgtt ccttttcttt gatctctgga tttaaaacga gatcatataa
      361 ttttagtacc atgttttcta tttcttcact tttagacatc acgatttcct cccaaaaata
      421 tatttcttcc cactacattc tataaacaaa gtatacgcta catctagatt tttttaaat
//
LOCUS       DAJSVP010000250          473 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSVP010000250 DAJSVP010000000
VERSION     DAJSVP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.261"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..419
                     /locus_tag="ON449_002820"
     CDS             <1..419
                     /locus_tag="ON449_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935322.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          163 a           93 c           82 g          135 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtccaaa ttattctaag att
//
LOCUS       DAJSVP010000251          472 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSVP010000251 DAJSVP010000000
VERSION     DAJSVP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.262"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..156
                     /locus_tag="ON449_002821"
     CDS             <1..156
                     /locus_tag="ON449_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3935323.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>472
                     /locus_tag="ON449_002822"
     CDS             175..>472
                     /locus_tag="ON449_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3935324.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVS"
BASE COUNT          206 a           36 c           84 g          146 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cg
//
LOCUS       DAJSVP010000252          430 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSVP010000252 DAJSVP010000000
VERSION     DAJSVP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.263"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>430
                     /locus_tag="ON449_002823"
     CDS             <1..>430
                     /locus_tag="ON449_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCR3935325.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAJSVP010000253          413 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSVP010000253 DAJSVP010000000
VERSION     DAJSVP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.264"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>413
                     /locus_tag="ON449_002824"
     CDS             <1..>413
                     /locus_tag="ON449_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935326.1"
                     /translation="VSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKL
                     DDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKG
                     TVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          169 a           61 c           94 g           89 t
ORIGIN      
        1 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
       61 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      121 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      181 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      241 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      301 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      361 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DAJSVP010000254          402 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSVP010000254 DAJSVP010000000
VERSION     DAJSVP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.266"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..355)
                     /locus_tag="ON449_002825"
     CDS             complement(<1..355)
                     /locus_tag="ON449_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935327.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAK"
BASE COUNT          138 a           73 c           40 g          151 t
ORIGIN      
        1 ctttagctaa aacatcaaat acatgtcctt gaaattgtat cgaggcagct ggatttagtt
       61 taaattgagg aaactttacc acagctgatt caaaacactt gtcacatttt tttatttttt
      121 tacctttgta aaaatattcg attgaatatt ctatttggca cgactcactt ggcagtatat
      181 gtatgtcaat atttttcatc tgtttcatag gtatttcaaa atctaaacta ttaaaaacat
      241 ataattccct gtaattattt acatctaact ctactaaatt tatctttgga acaatagatc
      301 ctccacccat tccaccataa taaaaaaaat cttgttctct atcaaaatag cacatattaa
      361 gctcagtttg tttatcatcc tcaataattt tataaatcaa ac
//
LOCUS       DAJSVP010000255          389 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSVP010000255 DAJSVP010000000
VERSION     DAJSVP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.268"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>389
                     /locus_tag="ON449_002826"
     CDS             <1..>389
                     /locus_tag="ON449_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3935328.1"
                     /translation="ISFEPTDSFQYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPA
                     ERTGINVFKNELNESRLKTYDTLLNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTD
                     NSSYPKPISDYINYLNNMKNRYEQKNV"
BASE COUNT          156 a           54 c           59 g          120 t
ORIGIN      
        1 attagctttg agccaactga ttcatttcag tatcttagca aagatattga aattgaagat
       61 atttataaaa ttttagctca agagctatct aaaaatgtta ttagtttgcc aaaggttttt
      121 tattttccag ccgaaagaac tggtataaat gtctttaaga acgaactaaa tgaaagtcgc
      181 ttaaaaacat atgatacctt gctgaatact gtgcaattta ctaatttaac taaccaaaaa
      241 aagaaagctg ccctcaagta cgatctgtta aaaaagaata ttgagctgtt aatggagaca
      301 acggataata gttcttatcc aaagccaata tcggattata ttaattatct gaataacatg
      361 aaaaatagat atgaacagaa aaatgtcaa
//
LOCUS       DAJSVP010000256          375 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSVP010000256 DAJSVP010000000
VERSION     DAJSVP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.269"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="ON449_002827"
     CDS             complement(<1..132)
                     /locus_tag="ON449_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3935329.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAJSVP010000257          364 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSVP010000257 DAJSVP010000000
VERSION     DAJSVP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.270"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>364
                     /locus_tag="ON449_002828"
     CDS             <1..>364
                     /locus_tag="ON449_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCR3935330.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DAJSVP010000258          351 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSVP010000258 DAJSVP010000000
VERSION     DAJSVP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.271"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(44..>351)
                     /locus_tag="ON449_002829"
     CDS             complement(44..>351)
                     /locus_tag="ON449_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3935331.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          103 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt t
//
LOCUS       DAJSVP010000259          350 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSVP010000259 DAJSVP010000000
VERSION     DAJSVP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.denovo.272"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..313)
                     /locus_tag="ON449_002830"
     CDS             complement(<1..313)
                     /locus_tag="ON449_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3935332.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KY"
BASE COUNT          130 a           67 c           35 g          118 t
ORIGIN      
        1 tatacttata aatccaccag tctcccataa catcagataa aggatttaac caaattgttc
       61 gattgattaa tgttgtaaat aaaatcatcg cagtatagaa tatcaaagca caaacttttc
      121 tatattttct atccttcttt aaatgaagat aaagttccat tagtatacgt ttatagccct
      181 cacctgcagc aatgccatct gttgcataca taaaatagac cagtcctaaa attgtcaata
      241 aaacagcaaa cccaaagggt tcatataatt tatataatat atctgtaata atttctgcaa
      301 tcactattat cataattatt tctccaatat caagtataca cttatcgctt
//
LOCUS       DAJSVP010000260        17995 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.048, whole genome shotgun sequence.
ACCESSION   DAJSVP010000260 DAJSVP010000000
VERSION     DAJSVP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.048"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            60..977
                     /locus_tag="ON449_002831"
     CDS             60..977
                     /locus_tag="ON449_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3935333.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            980..3157
                     /locus_tag="ON449_002832"
     CDS             980..3157
                     /locus_tag="ON449_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3935334.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3154..4155
                     /locus_tag="ON449_002833"
     CDS             3154..4155
                     /locus_tag="ON449_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3935335.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4152..5084
                     /locus_tag="ON449_002834"
     CDS             4152..5084
                     /locus_tag="ON449_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3935336.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5329..5445
                     /locus_tag="ON449_002835"
     CDS             5329..5445
                     /locus_tag="ON449_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3935337.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5461..7380
                     /gene="tet(M)"
                     /locus_tag="ON449_002836"
     CDS             5461..7380
                     /gene="tet(M)"
                     /locus_tag="ON449_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3935338.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7499..7666
                     /locus_tag="ON449_002837"
     CDS             7499..7666
                     /locus_tag="ON449_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3935339.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7726..8079)
                     /locus_tag="ON449_002838"
     CDS             complement(7726..8079)
                     /locus_tag="ON449_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935340.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8584..9006
                     /locus_tag="ON449_002839"
     CDS             8584..9006
                     /locus_tag="ON449_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3935341.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            9003..9233
                     /locus_tag="ON449_002840"
     CDS             9003..9233
                     /locus_tag="ON449_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935342.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9694..9897
                     /locus_tag="ON449_002841"
     CDS             9694..9897
                     /locus_tag="ON449_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3935343.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9979..11196
                     /locus_tag="ON449_002842"
     CDS             9979..11196
                     /locus_tag="ON449_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3935344.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11428..11754
                     /locus_tag="ON449_002843"
     CDS             11428..11754
                     /locus_tag="ON449_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3935345.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11822..12535
                     /locus_tag="ON449_002844"
     CDS             11822..12535
                     /locus_tag="ON449_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3935346.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12845..13696
                     /locus_tag="ON449_002845"
     CDS             12845..13696
                     /locus_tag="ON449_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3935347.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13817..15682
                     /locus_tag="ON449_002846"
     CDS             13817..15682
                     /locus_tag="ON449_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3935348.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15730..17139
                     /locus_tag="ON449_002847"
     CDS             15730..17139
                     /locus_tag="ON449_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3935349.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            17247..17948
                     /locus_tag="ON449_002848"
     CDS             17247..17948
                     /locus_tag="ON449_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3935350.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         5988 a         3095 c         3809 g         5103 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
      181 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
      301 aattacgggt tgagaatacg ccactaagta ccagtatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcacat ttattattca gtaatggtta tgtggagcag tctatcagct
      421 tagaaaaaca actgaacatt atacaggttg cggacttggt acttcccgac aaggaaactt
      481 cctttgagga atataccact atggagcttt tatccgttgc tatgctgatt gtcattagta
      541 cctttgcttt agactttatc catacagacc gaagcatttt caagattgta gatttagacg
      601 aagcatggag ctttttacag gtagcacaag gaaaaacact atctatgaag ctggttcggg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagac gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcatt tcgttccact gaccttaacg
      781 agattaaaaa gaccttagcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatagtaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggcttc cgtggaactg gtcggacggg attggaaaac aggtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ttgtatgttt ccaatgcgac
     1321 aggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
     1441 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
     1501 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
     1681 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
     1801 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
     2401 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
     2461 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
     2521 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
     2581 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
     2641 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
     2701 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
     2761 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
     2821 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
     2881 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
     2941 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
     3001 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
     3061 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
     3121 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
     3181 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
     3241 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
     3301 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
     3361 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
     3421 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
     3481 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
     3541 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
     3601 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
     3661 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
     3721 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
     3781 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
     3841 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
     3901 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
     3961 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
     4021 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
     4081 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
     4141 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
     4201 aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cataaaaagg
     4261 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
     4321 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
     4381 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
     4441 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
     4501 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
     4561 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
     4621 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
     4681 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
     4741 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
     4801 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
     4861 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
     4921 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
     4981 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
     5041 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
     5101 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
     5161 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
     5221 aaggtattat taaataaact gtcaatttga tagcgggaac aaataattgg atgtcctttt
     5281 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
     5341 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
     5401 aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac
     5461 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
     5521 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
     5581 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
     5641 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
     5701 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
     5761 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
     5821 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
     5881 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
     5941 tatcctaatg tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
     6001 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
     6061 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
     6121 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
     6181 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
     6241 aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg
     6301 gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt
     6361 gaattatgta agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt
     6421 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
     6481 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
     6541 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
     6601 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
     6661 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
     6721 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
     6781 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
     6841 gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg
     6901 atacgctatg gttgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
     6961 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
     7021 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
     7081 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
     7141 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
     7201 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
     7261 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
     7321 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
     7381 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
     7441 taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt
     7501 gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa
     7561 attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa
     7621 agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac
     7681 aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt
     7741 tcttgcttcg ttgataccgc tggctaaaga ttccattatg gatagttctt tgtctgtaaa
     7801 gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg
     7861 taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat
     7921 gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa
     7981 tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc
     8041 aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt
     8101 ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg tttataggtt
     8161 tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat ggtagtgcta
     8221 tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc
     8281 gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc aaaaaaagtc
     8341 gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat atagatagaa
     8401 taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg ctattcagaa
     8461 cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca ttgggactaa
     8521 gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa gggggtgaga
     8581 aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta tatctgtaaa
     8641 cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat tgcaaagcgt
     8701 gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac aacagataac
     8761 tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt tgaaaatgat
     8821 ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct actgctgttt
     8881 tactttatgg acatgagcga ttcagaaatt gcagacctgt tgaaattgaa ccgttctact
     8941 gtctatcggc atagaaccag tggactagcc ttaattaaaa agtttatgga ggaatttgaa
     9001 gaatgaaaac acaatatcct atgattccct ttcctctcat tgtaaaggca acagatggcg
     9061 ataccgaagc gattaaccag attctacatc attacagagg gtacataacg aagcgttccc
     9121 tacgacttat gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg
     9181 gaagaatgga aaccagactg attacaaaga ttttgtcatt tgaaattaag taatatcctc
     9241 tctcctttcg tggaagcgtg ctaaaccatt ccacgcttcc cgaacaggga ggtttgttat
     9301 tccaccaaag catattgagc tttcaatgtg ttttgatagg ctaacgagcc attgttcttt
     9361 gaaaactgaa taaaagtaat cgaatacgtt tcgataagaa aagagccaac ggaactaacc
     9421 gccatgacct atcttataaa gatagcgagc gattcatgtt agtgatccga gaagcaatct
     9481 ttagcaggat tgcctgcaac gacattctta tcgtgataat gatactccca tacagtcaat
     9541 agtccgagcg tgataaaacc gtcgcaggca atgagtatgg ctacatgaga accatgcagg
     9601 ggtggaactc ccgtgagctt tgctaaagct gttcgattgc tggtaaaaca acttttatga
     9661 aatccaaata agtgatttgg aaaggaggat tttatgaagc agactgacat tcctatttgg
     9721 gaacgttata ccctaaccat tgaagaagcg tcaaaatatt ttcgtattgg cgaaaacaag
     9781 ctacgacgct tggcagagga aaataaaaat gcaaattggc tgattatgaa tggcaatcgt
     9841 attcagatta aacgaaaaca atttgaaaaa attatagata cattggacgc aatctagcgt
     9901 cgccaaaggg tcttgtatat gataaaatag tattaagtcg tatcaaggct ctttccataa
     9961 aggaaaggag caaatgccat gtcagaaaaa agacgtgaca atagaggtcg aatcttaaag
    10021 actggagaga gccaacgaaa agacggaaga tacttataca aatatataga ttcatttgga
    10081 gaaccgcaat ttgtttactc gtggaaactt gtggctacag accgagtacc agcaggaaag
    10141 cgtgattgta tctcacttag agagaaaatc gcagagttac agaaagacat tcatgatggt
    10201 attgatgttg taggaaagaa aatgacactc tgccagcttt acgcaaaaca gaacgctcaa
    10261 agaccaaagg ttagaaaaaa cactgaaact ggacgcaaat atcttatgga tattttgaag
    10321 aaagacaagt taggtgtaag aagcattgac agtattaagc catcagacgc taaagaatgg
    10381 gctattagaa tgagtgaaaa tggttatgct tatcaaacca tcaataacta caaacgttct
    10441 ttaaaggctt cattctatat tgctatacaa gatgattgtg ttcggaagaa tccatttgac
    10501 tttcaactga aagcagttct tgatgatgat actgtcccta agaccgtact aacagaagaa
    10561 caggaagaaa aactgttagc ctttgcaaaa gctgataaaa cctacagcaa aaattatgat
    10621 gaaattctga tactcttaaa aacaggtctt cgtatttcag agtttggtgg tttgacactt
    10681 ccagatttag attttgagaa tcgtcttgtc aatatagacc atcagctatt gagagatact
    10741 gaaattgggt actacattga aacaccaaag accaaaagtg gcgaacgtca agttcctatg
    10801 gttgaagaag cctatcaagc atttaagcga gtgttagcga atcgaaagaa tgataagcgt
    10861 gttgagattg atggatatag tgatttcctc tttcttaata gaaagaacta tccaaaagtg
    10921 gcaagtgatt acaacggcat gatgaaaggt cttgttaaga aatacaataa gtataacgag
    10981 gataaattgc cacacatcac tccacatagt ttgcgacata cattctgtac caactatgca
    11041 aatgcaggaa tgaatccaaa ggcattacag tacattatgg gacatgctaa tatagccatg
    11101 acgctgaact attacgcaca tgcaacattc gattctgcaa tggcagaaat gaaacgcttg
    11161 aataaagaga agcaacagga gcgtcttgtt gcttagtagt acaaatgaat ttactactta
    11221 tttaccactt ctgacagcta agacatgagg aaatatgcaa agaaacgtga agtatcttcc
    11281 tacagtaaaa atactcgaaa gcacatagaa taaggcttta cgagcattta agaaaatata
    11341 aaaagataat tagaaattta tactttgttt tatgtaaaaa aatggtatga ttaatcaagt
    11401 aagaagtgat tggaagaagg caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga
    11461 gatgaacgta attggcgaca atttcataac gaaaaagatt tggctatctc tatttctttg
    11521 gaagcatctg aattgttaga actttttcaa tggaaaacgc cggaagaagt aaaagaaaca
    11581 caattagaac gaatcaaaga agaactggca gatgtattga tctattcata catgatggca
    11641 gacaaccttc acctggatct agatgagatc attgaagaaa aattgatcaa aaataatttg
    11701 aaatatcctg tagaaaaaag cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt
    11761 gtcattcggc tgttggaaag agcaaaagaa aaacagtaaa atgagtctgg agggagagaa
    11821 aatgaaaaaa aaaggaaaag caaaaagatg gctagtcaat atcctcttgt tcctcttgtt
    11881 attagtagga ctagctttga ttttcaatga acagatcaag gattattttg tcagagagac
    11941 aggagataaa tatgctatag cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa
    12001 agatgtcagt tttgactttg atgcggtaga accaatgacg acagaagggg tcatgaggtc
    12061 gcaaatgaga ggaacagatc taccagtgat tgctagtatc gcagtccctt cagtttcaat
    12121 caatttaccg gttttcaaag gcttggataa tacgtcacta ctttacggag caggaacatt
    12181 atctccagat caagaaatgg gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa
    12241 ccctgaattg ctgtttacac cgctagaaaa tttagagatg ggtgccaaga tctatttgac
    12301 agatttagaa aatgtgtata catacaaaac attttttaaa gaaaaagttg ctcctacaga
    12361 tacccaatta ctgaatgaag tcgagggaaa agaaattgtc acactaatta cttgtggcga
    12421 catggacgcc gtcacgcgac tagttgtaca aggagaactg gaaagtgtta catcgataaa
    12481 agatgcgaca gatgatatga gatctgcttt caatctagag acaaaaacat tttaggatac
    12541 tgggacataa gtcgttacag attaaaaaca gccaaaggat atgaatcgat aaggaaaaac
    12601 caatttgctg aaaaaaagag aaacgcaaag aggtgcatct ctgatttggt tgttttctag
    12661 atagacttgt gttccagttt ttttattttt tatatgaaaa ggcttgcata aataaaaatt
    12721 cgatggtatg atgattttga ataagaaatg gattgttact gtacggcagg caaaaccaga
    12781 attcagctaa gaaacagtag gcgaattcta ggttttttta tttttcttga gagggcgaag
    12841 caaaatgaaa attgataaaa ttttgaataa caatgtcgtc atttcgaaaa atggattcgg
    12901 ggaagaagtc gtttgtatgg gaaaaggact agcttttcaa aaaaagattg gggacgagat
    12961 ttcgccagaa gcggtccaaa aagaatttgt tttaagagat tcttttgcta aaaaccaatt
    13021 ccagcagctg atggctgata ttccggcaga ggagatcgaa tgggtcaaac aagtagtcga
    13081 actggctgaa gataaactaa aagttgaatt ttcgcctaat atttatctga cattgaccga
    13141 tcatcttcat tatgcgatca cacgagcaaa agaaaatatc caactgccta atccattatt
    13201 atttgaaaca aaaaaatttt atccgaaaga atatcaagca gcgaaagagg ctcttttgtt
    13261 cgtgaaacaa aagacgggtg tggaactttc tgaggatgaa gccggtttta ttgcctttca
    13321 ttttgtgaat agccaacaag gaaacgaaga catgcaagtg acgatgaccg ctaccacaat
    13381 ggtaagagat atactgagta tcatcagtaa attcttcggc atcgtgttcg atgaagaatc
    13441 tttgaattat cagcgtatca ttactcatct gcagtttttt gcccagcgtt atctcaaagg
    13501 ggaaggatct gacgagcaag atgaatttct ctacgcatta gttcaaggaa agtatccaaa
    13561 agcgtttcat tgtgtcgaac ggatcaatga gtatcttcga caaacgcaaa aacaggaaat
    13621 ggggatcgca gagcagattt atttaacgat tcatattcaa agagtagtaa gtgagaaaaa
    13681 aagcatccaa tcataaggat tgttactgta tggcaggcaa aacctggatt gaaaaaatag
    13741 gttcacgaat gtttgtgact gtttttttca atctgggttt ttatttttta aataaaaaag
    13801 aaagaaggaa aaaacaatgg ataatcaagc agttggtcgt cgtgtatggg aagcggtagg
    13861 aggacaaaag aatgtcaaaa gtttagtaca ttgtgctacg cggttgcgat tcagattaaa
    13921 agatgaatct ttagcagata cgcaaaagtt aaaagaagac ccggacgtga tccaagtcgt
    13981 acaaagtggc ggtcagtatc aagtagtgat tggcagtaat gtagcagatg tttatcaatc
    14041 gatcgtggat gaggaaggtc ttgcagtttc ggaggatgga aaagaagagt ccaaaaatat
    14101 tttgaatcgt ctgatcgata ttatctctag tatatttaca ccttttctag gggcgatggc
    14161 agccgccggg gtattgaaag gattcttatc attggcaacg gtgatgggct ggctgacagc
    14221 tgatagcggt acttatcaaa tattgtttgc tgcagcagat ggtgtcttta ccttcttacc
    14281 tgtcatgctt gcttttacag cagctaaaaa gttcaaagcc aaccagtttt tagcagtagc
    14341 aattgctatg gcacttgtgt atccagctat cacagcagtt gctggtgcgg gagaagctat
    14401 cagtttcttt gggatcccag tgattctttc accatctggt tatacttctt ctgtcatacc
    14461 aatcattcta gccgtatggg ttcaaagtaa attagagcca ttcgtaaaaa aagtgattcc
    14521 gcaatttctg caaatgattt tagttccgct tgtcgtttta gtagtgatgg ttccattaac
    14581 atttcttgct ttaggaccaa tcgggacggt tgccggtaat gctttaggcg gtctcttcaa
    14641 ctcgatttat ggatttagtc caatcgtagc aggtctaatc atgggaagtt tatggcaagt
    14701 attcgtgatg ttcggcatgc attggggatt cgtaccgatc atgtttttga atatcgaaca
    14761 atatgggttt gatgtattga tgccaatgct tcttccagcc atcttggcac aaggtggggc
    14821 tgctttagct gtagcattgc gaacaaaaga tacgaaactt cgagcattag ggatttcttc
    14881 tacagtgact tcgttatttg gaatcactga accaactgtg tatggtgtga cattaccttt
    14941 gaagaaacca ttcattgccg cttgtatttc tggaggtatt ggtggagcta ttatcggatt
    15001 ttccggagta aaagctttct ccagcagttt ggtcagctta ttgacgattc cgactttcat
    15061 taatacggtg gatggtgtag agtcaaatgt gacagtagca gtaatagcta caggaattgc
    15121 ttttgttctt gcattcgtag gtacattgat tttaggattt gacgaacaaa cacaagacaa
    15181 tcaattagaa aacaaacatg cgaatgcagg agaaccgatt acttcagcac ggcatacctt
    15241 aaaaagtcct ttaacgggta aagtactgcc attgagtgaa gtaccagacc aagttttttc
    15301 ttctggcgtg atgggaaaag gtatcgctat tgatccagaa gtaggagaac tagttgcacc
    15361 tgcagacgga gagatcacga ctatttttcc aactggacat gctgtaggga tcacaacgac
    15421 tgacggagcg gagattttga ttcatatcgg gatggataca gtagaattaa atgggaatgg
    15481 ctttgagatc ttagtcaaac aaggggatct cgtcaaagca ggagatttat tgattcgttt
    15541 tgacatcgaa gcaattagag cagctggcta tagcgtaatc actccagttg tcattacgaa
    15601 tactgatgca tttgccgata tacttgaatt agaccaaaaa gagattattg caaacgaaga
    15661 tgttctggca attgtaaaat aaggaggaaa tcaggatgca atcacgtttt tcagaaaaat
    15721 ttttatgggg tggagcaact gctgccaaat caattagaag gggcttatga tcgagacgga
    15781 aaaggactat ccgttgcaga tgctatgcct ggaggtaagc agcgctttgc gattatcggc
    15841 agtgaagaat ttgactggac gattgatcaa gaaaaatata tttacccgaa tcatcgagga
    15901 atcgatcatt acgatcgttt caaagaagat cttgccttgt ttgcggaaat gggattcaaa
    15961 tgttatcgtt tttcgattgc ctggacacga atttttccaa atggagacga aggtactcct
    16021 aacgaagcgg gattagaatt ttatgatcag ctgattgatg agtgcttaaa atatgatatt
    16081 gaacctgtga tcacgatttc tcattacgaa atgcctcttc atttggcaaa agaatacggt
    16141 ggctggaaaa atcgaaaact gatcgacttc tatgaacgat ttgctcagac agtattagag
    16201 agatacagca gtaaagtcaa atattggatg actttcaatg aaatcaattc ggcctttcac
    16261 tttccagctt taagtcaagg attagtaaaa agcaatggag caggagagta ccaaaatatt
    16321 ttccaagcat ggcataatca attcgttgca agtagcaaag ccgtaaaaat cggccacgaa
    16381 ttgcgttcgg atatccaaat cggctgcatg attatctatg caacaactta cagcatcgat
    16441 gccaatccag tcaatcaggc agcaacaatg atccaaaacc aagaattcaa ttttttctgc
    16501 acggatgtcc aagtacgtgg agaataccct gcatacacag cgcgtaccca taaaaaatat
    16561 ggagtcgatc cggaaaaatt agagcaaacg gaaaaagact tccagttgct aaaagaatat
    16621 ccagtggact acatcggatt tagttattat atgtctacgg caatcaatga gactgatcca
    16681 caagcagcaa cttcagaagg gaacttgctg ggaggagtaa aaaatccgtt tcttgaggca
    16741 agtgaatggg gctggcagat cgatccagaa gggctgagaa tcgcattgaa tgaattatac
    16801 aatcgttacc agaaaccatt attcattgtt gaaaacggtc ttggagccat tgatcatatg
    16861 gaagaggatg gcacgattat tgatgattat cggatcgatt atttacgtcg tcatatcgaa
    16921 gcgatggcta atgcagtagc agacggtgta gatttgatgg gctatacgcc ttggggatgt
    16981 atcgatcttg tcagtgcatc aacaggagaa atgagtaaac gttacggctt tatctatgtg
    17041 gatctagacg atgaaggaaa tgggacactg aatcgatcaa agaaaaaatc ttttgattgg
    17101 tataaagaag taattgcctc aaatggcgta aacttgtaat taaagcagct gaatgaaaaa
    17161 gaacacactt ctcacatgtg atttgtgttc tttttttatt ttttatgtgc tattctttta
    17221 gtaatagtaa agcaaagggt gacgttatga aaaggacacg tgtactatac ttggaagtcg
    17281 cagataaaat taaagaagat attttctcag gaaaatatcc agtcggttcg atgttgccga
    17341 cagaaagtga attagaagaa ctgtttcaag tcagcaaaat cactgtccga aaggcaatcg
    17401 aactgctggc atcagatgaa tatgtcgcaa agaaaagtgg tcgtgggacg acggttctaa
    17461 gcaatcgtcc ttacaatcga ttatccaaag cggcaagctt tacgcaaatc ttggagaact
    17521 cccgatatga agtccggaaa gaaacgctgg atttgcaatt ggtccatata aagaaggaag
    17581 atcctttgtt ttcctttttc ggtgagcaag ctgtttgttt taagcgctta tattatttga
    17641 acaatcagcc ttatatttat tttgttcatt atcttccaca agaaatgatg caattcagca
    17701 gagaagaatt tggcaaagaa tctttgtatc gtttgctgac gaaaaaagga tatactattg
    17761 actcattttc tgatgatttc tcggcagtat ttttaacaga agaagaacaa ggaattctga
    17821 aaacagacgc acagatcggc atcaaacgga tacggaaatc gtctacgcaa attggggaaa
    17881 tgattgaata ttcggaggcg gtctaccata cggagctgca tccttaccat attgattatg
    17941 aaacataaat aaaaaaagag gttgtgaaaa agctgccctg catcaagtaa taaga
//
LOCUS       DAJSVP010000261        17108 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.052, whole genome shotgun sequence.
ACCESSION   DAJSVP010000261 DAJSVP010000000
VERSION     DAJSVP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.052"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(134..577)
                     /locus_tag="ON449_002849"
     CDS             complement(134..577)
                     /locus_tag="ON449_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3935351.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ON449_002850"
     CDS             complement(795..2135)
                     /locus_tag="ON449_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3935352.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ON449_002851"
     CDS             2404..3300
                     /locus_tag="ON449_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3935353.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ON449_002852"
     CDS             complement(3450..4391)
                     /locus_tag="ON449_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3935354.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ON449_002853"
     CDS             4517..5638
                     /locus_tag="ON449_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3935355.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON449_002854"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON449_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3935356.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="ON449_002855"
     CDS             7426..7992
                     /locus_tag="ON449_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3935357.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ON449_002856"
     CDS             8203..9264
                     /locus_tag="ON449_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3935358.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ON449_002857"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ON449_002857"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3935359.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ON449_002858"
     CDS             10177..12159
                     /locus_tag="ON449_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3935360.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON449_002859"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON449_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3935361.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ON449_002860"
     CDS             12881..14305
                     /locus_tag="ON449_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3935362.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ON449_002861"
     CDS             complement(14486..15259)
                     /locus_tag="ON449_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3935363.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ON449_002862"
     CDS             complement(15256..16161)
                     /locus_tag="ON449_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3935364.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ON449_002863"
     CDS             complement(16315..16635)
                     /locus_tag="ON449_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3935365.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5539 a         3105 c         3498 g         4966 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatat
//
LOCUS       DAJSVP010000262        13213 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.068, whole genome shotgun sequence.
ACCESSION   DAJSVP010000262 DAJSVP010000000
VERSION     DAJSVP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.068"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(204..2162)
                     /locus_tag="ON449_002864"
     CDS             complement(204..2162)
                     /locus_tag="ON449_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3935366.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ON449_002865"
     CDS             complement(2159..3031)
                     /locus_tag="ON449_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3935367.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ON449_002866"
     CDS             complement(3373..4245)
                     /locus_tag="ON449_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3935368.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ON449_002867"
     CDS             4373..4942
                     /locus_tag="ON449_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3935369.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ON449_002868"
     CDS             5009..5746
                     /locus_tag="ON449_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3935370.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ON449_002869"
     CDS             5977..7245
                     /locus_tag="ON449_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3935371.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ON449_002870"
     CDS             7732..8196
                     /locus_tag="ON449_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3935372.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ON449_002871"
     CDS             8180..8809
                     /locus_tag="ON449_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3935373.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ON449_002872"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ON449_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3935374.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ON449_002873"
     CDS             10688..11134
                     /locus_tag="ON449_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3935375.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ON449_002874"
     CDS             complement(11333..11848)
                     /locus_tag="ON449_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3935376.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ON449_002875"
     CDS             11965..13167
                     /locus_tag="ON449_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3935377.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAJSVP010000263        11340 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.081, whole genome shotgun sequence.
ACCESSION   DAJSVP010000263 DAJSVP010000000
VERSION     DAJSVP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.081"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(230..466)
                     /locus_tag="ON449_002876"
     CDS             complement(230..466)
                     /locus_tag="ON449_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3935378.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(649..1035)
                     /locus_tag="ON449_002877"
     CDS             complement(649..1035)
                     /locus_tag="ON449_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCR3935379.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(1017..2345)
                     /locus_tag="ON449_002878"
     CDS             complement(1017..2345)
                     /locus_tag="ON449_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3935380.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2306..2635)
                     /gene="mobC"
                     /locus_tag="ON449_002879"
     CDS             complement(2306..2635)
                     /gene="mobC"
                     /locus_tag="ON449_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR3935381.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2893..3264)
                     /locus_tag="ON449_002880"
     CDS             complement(2893..3264)
                     /locus_tag="ON449_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3935382.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            3409..3594
                     /locus_tag="ON449_002881"
     CDS             3409..3594
                     /locus_tag="ON449_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3935383.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3752..3919
                     /locus_tag="ON449_002882"
     CDS             3752..3919
                     /locus_tag="ON449_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935384.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4537..5196
                     /gene="vanR"
                     /locus_tag="ON449_002883"
     CDS             4537..5196
                     /gene="vanR"
                     /locus_tag="ON449_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCR3935385.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5199..6542
                     /gene="vanS"
                     /locus_tag="ON449_002884"
     CDS             5199..6542
                     /gene="vanS"
                     /locus_tag="ON449_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3935386.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            6713..7519
                     /locus_tag="ON449_002885"
     CDS             6713..7519
                     /locus_tag="ON449_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HCR3935387.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            7537..8364
                     /gene="vanW"
                     /locus_tag="ON449_002886"
     CDS             7537..8364
                     /gene="vanW"
                     /locus_tag="ON449_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCR3935388.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            8361..9332
                     /locus_tag="ON449_002887"
     CDS             8361..9332
                     /locus_tag="ON449_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCR3935389.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            9325..10353
                     /gene="vanB"
                     /locus_tag="ON449_002888"
     CDS             9325..10353
                     /gene="vanB"
                     /locus_tag="ON449_002888"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCR3935390.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            10359..10967
                     /locus_tag="ON449_002889"
     CDS             10359..10967
                     /locus_tag="ON449_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCR3935391.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(11033..11206)
                     /locus_tag="ON449_002890"
     CDS             complement(11033..11206)
                     /locus_tag="ON449_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935392.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2972 a         2566 c         2904 g         2898 t
ORIGIN      
        1 ctacgcattt agaacccggt attgtcgggg ataggatgaa gtattcgctt gcccctgcaa
       61 acatgcggga gaagccttgc agcgggcggt tttcaatccc ttaaatgcgt aggttgcagc
      121 tataattaga cccctgaaac agatatagcc gcgcagcggg tgtatctgtt tcaggggtgt
      181 gcaggtgata gccgcaaagc ggcgtaagag ggtgcagccc tcttgccgtc tatctctgtt
      241 ccagcgggaa gctcggcaat gcttccagca gtttcaggga aatgcgctgc cgtatatctt
      301 cgtcaatgac tttcttcttt ttgccgtttg cctgtctgat ctccaccgtg gaaagctcgt
      361 tgatgtagtc agcataatgg gccaacactt tttccgtggc ccacctctca ccggcaacgg
      421 cggctttgat cgtttcatag tccagcagct tttcttgtcc gtccatcctg atacccccgt
      481 cagactgatt ttttctatga taccatattc ggaagcatgg aaccagagaa aacggcgctt
      541 ttctgctggt aaattctacg catttagaac agcacttcgg cggggatagg ataaaacacc
      601 cacatacccc gctgaccatg ccggaaaagc cttgaaatat aagggctttt acagctctaa
      661 gtgcgtaggt ttccagtatc ttttccgctg gcgctcggcc tctttgcggc ggcgcatccg
      721 tttggagcag gccgcgcagt atttggcccg gttagacttc ggcgtgaacg tggccccgca
      781 ctcacagcag cgtttcactt cgtcccggtc tttggtgatc tcggcataca gggccgcatc
      841 cagcgggagg acagcggcct taaaccagcg gcacagaagc gaataggaaa tgctctgtac
      901 gcaaatacat tcttcgccgt cgtccagcaa caggcagttg ccgtcacaat agttgcagca
      961 ctcatgcgtc agcttccggg ccttgcgata ttgagggtaa ttcattcgtg gaatactcat
     1021 agctcttgcg tccattcctt tcccggcgtc atacgcaaaa tgccgtccac attctgcttg
     1081 atgatgccgt attcctgcat ctgctttttc gcttcgccgt acttctcata cagccgttcc
     1141 ttttcctcgg ccaatcgctt gtactcggct ttcagggcgt acagatcggg gagctttttc
     1201 acaccctgtc cctttagggc ctttgccgca gcttcataca aaatcaacgt gctttcatgc
     1261 tcacgccgga attttgcctt atccgctgca ttgcggtatt ccatagccac gggccagagc
     1321 tgcttgtagg tagaaatatt ctttatcagc accgccatat ccgcaagccg gtgttccata
     1381 gctttgaggg tagcggcggc ctcgtcgctg gcggcggtga tctcggccac tttgctttcc
     1441 aaatccgggt aggtgtctat cccatgttca gtcaggaaat tcaaagtctt tgcggcctgc
     1501 ttcaaattgt ggagcttcgc ccacctctca tagcctgccg actgctgggc cttaatgttg
     1561 ttttccagat caatccgcaa agaaatcccc ttgcgctctt tgggcgctct ggtgataacg
     1621 cggcctttga tacgctcttt gatggcttcc tcggtgtagg cttcccccaa tgtcttgcag
     1681 cgggtaaagc gttcctgtcc gggagcgcgg aaagaaataa actttcccgg cttgatctca
     1741 tagccctgcg cctgcatgag ccgcaggaaa tcatcaaaat cttttgcctg cgggatagcg
     1801 gcgtcaatgg ctgctttgag cttcgctttc cagctcgtcc ctttccgctg ggcgtcccac
     1861 tcggcatagc tcttgccctt actctggccg ggcttcacca cggacaatcc gttttccttg
     1921 cacagcctgt cgctggtgcg ccggatatag gcatagctct gtttgttgga aatgtacttg
     1981 cgctgattta ccatatccac ggcacagaaa ataatgtgat tgtggacgtg gcccttatca
     2041 atatgggtag tcagcacata gggatatttc ccttgcagca cttcctcggc aagctgcttc
     2101 ccgatctcat gggcctgttc cggcgtggct tcgccggggg caaaggattg tatcaggtgg
     2161 tggcccagat tattgccctt ttggtatgcc tgcgccagca gcatttcaaa ttcaatgtcc
     2221 gctgtttcat aagagcaggc aaaggaggcc accagcagct tgccgtcagt tttgtccggg
     2281 ttctcgatgt agtcaagggc ttttttcagc gttgacttga taggatgtat ctttgtaact
     2341 gccatatctg cgccagcgcc ccctttatat cctcgatgtc ctgtgcgtac accggcccca
     2401 taccgttcag cctctttgca atctgattga cgttgacgcc gatcttctgt atctcggcgg
     2461 tctgcgcccg gatgtcgctg gtgtccatgt tgacgatata gccgtcaatg agcatcttcc
     2521 gggcgtaggc tgaaaagttc ttcgtatgca gcagcgccat tttctgttca atcatagccc
     2581 gttcctccgg cgtcacaggg acgcgcagca cgatttttcg ctttctgttt gccatgcttc
     2641 accgtccttt ccattcaggg gtttggggat ttccccaaca agcaaaacgg acgcggcccg
     2701 gcagggacgg ggcaggccgt ttttccggga gtggctacac tccctatgct tgctacgtta
     2761 ctgtttctcc actaacaaga cgtttccaaa gccccaaact acccgcaaaa tagccctctg
     2821 gaaagaaaaa agtgcaaaaa aatagaagct acgctgtgca gctcccgcta aaagtaaaac
     2881 agaggatgcc agttacacat cctccgtttc attcttcttc ggcttttcaa ttagtatggc
     2941 gctgtcaatc gcgccctcga tgatcggcag gtattcttcg gggcagagtt tcagcttgtg
     3001 gctgaccctt tgccgcagtt cgctttcctc ccgcatgatc tccgggttaa agtagcgttc
     3061 cacaggaagc ccgcaggctt taataagctg tatgacaacc ggcagactgg gaatggcccc
     3121 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
     3181 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
     3241 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
     3301 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
     3361 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
     3421 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
     3481 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
     3541 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
     3601 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
     3661 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
     3721 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
     3781 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
     3841 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
     3901 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
     3961 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
     4021 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
     4081 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
     4141 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
     4201 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
     4261 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
     4321 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
     4381 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
     4441 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
     4501 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
     4561 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
     4621 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
     4681 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
     4741 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
     4801 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
     4861 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
     4921 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
     4981 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
     5041 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
     5101 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
     5161 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
     5221 ttaaggtttt ttcctatacg atcattgtcc tgctactggt tgtcggtgta acagcaacac
     5281 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
     5341 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
     5401 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
     5461 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc gactttcttt
     5521 atgtggtaca tagagatgat aatatttcga ttgttgctca aaacaaggca ggtgtgggat
     5581 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
     5641 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
     5701 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
     5761 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
     5821 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
     5881 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
     5941 gaatgcttgg aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
     6001 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
     6061 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
     6121 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
     6181 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
     6241 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
     6301 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
     6361 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
     6421 gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc
     6481 tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt
     6541 aaatatttaa aacttaaatg attttgaccg acaggtataa ccctgccggt ctttttttgc
     6601 tgctacagga aaactacaga ctggctacag ggaaagtaca gatgcgcttg ccataataac
     6661 aatcgtacca gccacaaatc gtagttttat tgcaaaggag gcattcgatc aaatggaaaa
     6721 aagcaactat cattccaatg cggatcatca caaacggcat atgaaacaat ccgtggaaaa
     6781 gcgggctttt ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt ttttggggtg
     6841 gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa ctcatacagg
     6901 cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg ccaaagaaca
     6961 gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca tccccgcaca
     7021 gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc ggatttctcc
     7081 ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga ttgtcgcatc
     7141 cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg ccgaatataa
     7201 ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg tggccgtgcc
     7261 gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg gaattcattc
     7321 aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg gttttattcg
     7381 tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt ggcattaccg
     7441 atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc ttgaggaata
     7501 tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag attgacacag
     7561 cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga
     7621 atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct ttcccatttg
     7681 ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat gatataccag
     7741 gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt attgataaga
     7801 ccgggggaaa ccttttcttt ctggtggctg gtacgccatg cggacaaaga taccccctat
     7861 aaagacggcc ttacggtgac caatggtaaa ctcaccacca tgtcgggcgg cggtatgtgc
     7921 cagatgagca atttactatt ttggatgttc ctgcatacgc cattgacaat tatccagcgc
     7981 agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa aggggtggat
     8041 gcaaccattc cagagggctg gattgattta aaagtgcgaa acgataccga ctgcacctac
     8101 caaatatggg tgaccttaga tgatgagaaa atcatcggtc aggtgtccgc cgacaaagag
     8161 ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga aagtggcggg
     8221 atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg ggaaataata
     8281 gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg
     8341 gatattcagg aggagaacca atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac
     8401 aggatgaggc aaatgctttc cgcgctttat cgccggattt tcatattatc cctacgctga
     8461 ttagcgatgc gatatcggca gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg
     8521 taggccataa gtccgaggtt tccgaggcga caattcttgc gctgagaaag gtcggggtaa
     8581 aatacatttc tacccgcagc atcggctgcg atcacattga tacgactgcc gccgagagaa
     8641 tgggaatctc ggttggcacg gttgcgtatt cgccggacag cgttgcggat tatgctttga
     8701 tgctgatgct gatggccata cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag
     8761 atttcagatt ggatcgtatc cgggggaaag aactggggga tatgactgtg ggagttattg
     8821 gaaccggcca tatcgggcaa gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc
     8881 tggcctatga taacagccga aaaatggatg cagattatgt ccagcttgat gagcttctaa
     8941 aaaacagcga tattgttacg ctccatgtgc cgctttgtgc ggatacccgc catctgatcg
     9001 gtcagaagca aattggagag atgaagcaag gcgcattttt aatcaacacc gcgcgcggag
     9061 cgcttgtcga taccggggcg ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg
     9121 cactggatgt gttggagggc gaggatcagt ttgtttatac cgattgctcg cagaaagtgc
     9181 ttgaccatcc ctttttgtcg cagcttttaa ggatgccgaa tgtgatcatc acaccccata
     9241 cggcgtacta cactgagcgt gtgctgcagg atactacaga aaaaacaatc aggaattgtc
     9301 tcaattttga aaggagttta cagcatgaat agaataaaag tcgcaatcat cttcggcggt
     9361 tgctcggagg aacatgatgt gtcggtaaaa tccgcaatag aaattgctgc gaacattgat
     9421 acggaaaaat tcgatccgca ctacatcgga attacaaaaa acggtgtatg gaagctatgc
     9481 aagaagccat gtacggaatg ggaagccgac agtctccccg ccatactctc cccggatagg
     9541 aaaacgcatg ggctgcttgt catgaaagaa agcgaatacg aaacacggcg tattgatgtg
     9601 gctttcccgg ttttgcatgg caaattcggg gaggatggtg cgatacaggg gctgtttgta
     9661 ttgtctggta tcccctatgt gggctgtgat attcaaagct ccgcagcttg catggacaaa
     9721 tcactggcct acattcttac aaaaaatgcg ggcatcgccg ttcccgaatt tcaaattatt
     9781 gataaaggtg acaagccgga ggcgggtgcg cttacctacc ctgtctttgt gaagccggca
     9841 cggtcaggtt cgtcctttgg cgtaaccaaa gtaaacggta cggaagaact taacgctgcg
     9901 atagaagcgg caggacaata tgatggaaaa atcttaattg agcaagcgat ttcgggctgt
     9961 gaggtcgggt gtgcggtcat ggggaacgag gatgatttga ttgtcggcga agtggatcaa
    10021 atccggctga gccacggtat cttccgcatc catcaggaaa acgagccgga aaaaggctca
    10081 gaaaatgcga tgattacagt tcccgcagac attccggtcg aggaacgaaa tcgggtgcag
    10141 gaaacggcaa agaaagtata tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt
    10201 tttttgcagg aggatggcgg catcgttcta aatgaggtca ataccatgcc cggttttaca
    10261 tcgtacagcc gctacccacg tatggtggcc gccgcaggaa tcacgcttcc tgcactgatt
    10321 gacagcctga ttacattggc gttaaagagg tgataagtat ggaaaatggt tttttgtttt
    10381 tagatgaaat gttgcatggc gtccgttggg atgccaagta cgccacatgg gataacttca
    10441 caggaaaacc ggtggatggg tatgaggtga atcgcatcat cggcacaaag gccgtggcgt
    10501 ttgctctgcg cgaggcacaa atccatgcgg ctgcgcttgg ctatggcttg cttttatggg
    10561 atggatatcg gccaagaact gcggtggact gcttcctgcg ttgggcagcg caaccggagg
    10621 acaatctcac aaaagaaaaa ttttacccca atatagagcg agccgagttg attacaaagg
    10681 gttatgtggc ctcacaatcc agccatagcc gtggaagcgc aattgatctt acgctctacc
    10741 acctggatac aggggaactt gtttcaatgg gaagtaactt cgattttatg gacgaacggt
    10801 cgcatcatac agcaaaaggg ataggggatg cagaggcaca aaatcgaaga tgcttgcgta
    10861 aaatcatgga aagcagcgga tttcagtctt atcgctttga atggtggcac tataagttga
    10921 ttgatgagcc ataccccgat acctatttta attttgccgt ttcataatga aagtattggt
    10981 tttgtatcag attttggatt tgataaaatc tatgctggtc atggacaatc ttttactgga
    11041 aagtggttaa agcatctttt gtaatacatt gcaaattata gtattcaatc tgcccagcgg
    11101 cagaaaagaa aaaaaccgct gttgggcaga cctttcccac gcccattttg tattttgctg
    11161 gcggcggttt tgagaaatca aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc
    11221 cataggagga tgccgctatg ctgttttgct attgccatgc tctaatccac ttccaccact
    11281 caaaaaagcc ttgcccctcc atgaactgaa ccccaaaagt tgaactattt aatggactgt
//
LOCUS       DAJSVP010000264         5792 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.122, whole genome shotgun sequence.
ACCESSION   DAJSVP010000264 DAJSVP010000000
VERSION     DAJSVP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.122"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..1408
                     /locus_tag="ON449_002891"
     CDS             2..1408
                     /locus_tag="ON449_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935393.1"
                     /translation="MDFFIYTKYFILSSFYYGEKFFKKIDAAFLFSCLYTFATYRTVD
                     IYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSAL
                     IACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFK
                     TDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVS
                     TWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLII
                     GATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKE
                     RPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVV
                     NDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL
                     KDSRYVTKKEINKKNENK"
     gene            2044..2541
                     /gene="dfrG"
                     /locus_tag="ON449_002892"
     CDS             2044..2541
                     /gene="dfrG"
                     /locus_tag="ON449_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3935394.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2613..4565)
                     /locus_tag="ON449_002893"
     CDS             complement(2613..4565)
                     /locus_tag="ON449_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3935395.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4572..4808)
                     /locus_tag="ON449_002894"
     CDS             complement(4572..4808)
                     /locus_tag="ON449_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3935396.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5046..5792
                     /locus_tag="ON449_002895"
                     /pseudo
     CDS             5046..5792
                     /locus_tag="ON449_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1853 a          944 c          961 g         2034 t
ORIGIN      
        1 tatggatttt ttcatttaca caaaatattt tatactctct agtttttatt atggagaaaa
       61 gttttttaaa aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta
      121 tcgaacagtt gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat
      181 cccagctgtt ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc
      241 tttatctgtc agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag
      301 tgcattgatt gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga
      361 tataaaattt gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag
      421 ctatttttgg tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa
      481 aacagatttg cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa
      541 cgatattaca acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt
      601 catactgaaa gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg
      661 ggtatgcgta actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact
      721 ccagtttcct tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct
      781 tgtttttatg aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt
      841 ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc
      901 aaaacacggt catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat
      961 gggaggatta tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa
     1021 acatgaagta aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa
     1081 cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta
     1141 tttaggtaca caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg
     1201 cttagtgaat gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac
     1261 tccaactgct ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc
     1321 aagttttaaa cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga
     1381 gataaacaag aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag
     1441 ttagtagtat atattctttc atctttttca aaaatacaca gagtatttat accactggag
     1501 atctttcttt tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta
     1561 attatgagac attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt
     1621 tttttccagc tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt
     1681 acgtttggtt attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt
     1741 taaaacaaaa aaaagcggca tttttatttt aagtagttca gttttggagt acaaaatgac
     1801 aaaatgtact tcaaaatgga ctactttttt cattttaact tacataccaa attacataca
     1861 aaatgattta taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg attttgtggt
     1921 attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca ggataatgag
     1981 aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta
     2041 taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat
     2101 gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat
     2161 ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga
     2221 aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca
     2281 atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa
     2341 cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat
     2401 gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt
     2461 gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa
     2521 agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca
     2581 caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa
     2641 tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct
     2701 gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca
     2761 tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat
     2821 ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg
     2881 tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt
     2941 ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta
     3001 tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga
     3061 cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat
     3121 cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat
     3181 tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt
     3241 aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc
     3301 attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta
     3361 taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat
     3421 tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa
     3481 gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt
     3541 cttgtatctt catttaattc atccattgaa atattccaat cataggcaac atcagaacgg
     3601 aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct
     3661 tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag
     3721 tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc
     3781 tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag
     3841 gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc
     3901 atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct
     3961 ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg
     4021 cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct
     4081 tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt
     4141 tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag
     4201 ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta
     4261 tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg
     4321 tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga
     4381 tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt
     4441 gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt
     4501 gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg
     4561 gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta
     4621 cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat
     4681 tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc
     4741 cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga
     4801 aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca
     4861 atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat
     4921 tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca
     4981 gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact
     5041 acttattttt atttagttgt ctctatattt tctctgctta tcggacagta gacatctact
     5101 atcgttccgc aattgccgaa gctatcgcaa taacacttgt tatccctgta ttattttatg
     5161 cttatcaaat catctctgga aaagaagaaa agtatccgtc tgtaaagcta gcattgagta
     5221 tgagtttgct agtgtacagc catgtactat caacattaat gagtacagca ttaattatca
     5281 tttttatttt tattcgtttg gtttctaaag gtttcaagaa tgcagatttt attgctatat
     5341 ttaaaaaatt atttagtgcg gcaggaatga cattggttct gacgagtgct ttttggtatc
     5401 cgatgtttga acaaatgctc taccaaaaaa tcaacaaacc tagcgtgaca aatttatatg
     5461 ctcatgcatc taatgtgttc gatagtctga ctgaagctat gaacaacgac ttaactactt
     5521 actcaatggg actagttggt ctgttatctt tatgtattcc cctgatttta tttaaaaaat
     5581 taacaaacat agaaaaaaag atttattatg gtacgtgttt gacttggcta gctacaacta
     5641 gtttggtgcc atggtatctt ctgcaaaata cgccagccaa attgttacaa tttccttggc
     5701 ggatcttaag cttgcaaata atattctctt ctctgatatt aacgatgata tttttcaaaa
     5761 atagaagata caataagaca agagaactat tt
//
LOCUS       DAJSVP010000265         3814 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.146, whole genome shotgun sequence.
ACCESSION   DAJSVP010000265 DAJSVP010000000
VERSION     DAJSVP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.146"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="ON449_002896"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="ON449_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3935397.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="ON449_002897"
     CDS             complement(1443..2546)
                     /locus_tag="ON449_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3935398.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="ON449_002898"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="ON449_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3935399.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1247 a          648 c          764 g         1155 t
ORIGIN      
        1 atcaattctg attcatcagt cccataaatt atagagccat cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     3721 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     3781 tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DAJSVP010000266         1500 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.197, whole genome shotgun sequence.
ACCESSION   DAJSVP010000266 DAJSVP010000000
VERSION     DAJSVP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.197"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            390..473
                     /locus_tag="ON449_002899"
     CDS             390..473
                     /locus_tag="ON449_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3935400.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            598..1335
                     /gene="erm(B)"
                     /locus_tag="ON449_002900"
     CDS             598..1335
                     /gene="erm(B)"
                     /locus_tag="ON449_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3935401.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          550 a          223 c          245 g          482 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattaaaa attttgtata ataggaattg aagttaaatt agatgctaaa
      361 aatttgtaat taagaaggag ggattcgtca tgttggtatt ccaaatgcgt tatgtagata
      421 aaacatctac tgttttgaaa cagactaaaa acagtgatta cgcagataaa taaatacgtt
      481 agattaattc ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa
      541 acaaatcgtt taacttctgt atttgtttat agatgtaatc acttcaggag tgattacatg
      601 aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata
      661 ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg
      721 catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt
      781 catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac
      841 caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt tgggagtatt
      901 ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca tgcgtctgac
      961 atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta
     1021 gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc
     1081 tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat
     1141 gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa
     1201 tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac
     1261 aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt
     1321 aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa
     1381 agggaatgta gataaattat taggtatagg gcacctctaa taactaccaa ttaattcacc
     1441 tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc tatttcttat cgatacaaat
//
LOCUS       DAJSVP010000267         1220 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022824
            SAMN29549701-rid15780273.guided.211, whole genome shotgun sequence.
ACCESSION   DAJSVP010000267 DAJSVP010000000
VERSION     DAJSVP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549701
            Sequence Read Archive: SRR20032282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473846.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473846.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:03:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,902
            CDSs (total)                      :: 2,837
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 25 of 57
            Pseudo Genes (incomplete)         :: 29 of 57
            Pseudo Genes (internal stop)      :: 11 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549701-rid15780273.guided.211"
                     /strain="AUSMDU00022824"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            47..439
                     /locus_tag="ON449_002901"
     CDS             47..439
                     /locus_tag="ON449_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3935402.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..>1220
                     /gene="aac(6')-Ie"
                     /locus_tag="ON449_002902"
     CDS             440..>1220
                     /gene="aac(6')-Ie"
                     /locus_tag="ON449_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCR3935403.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNI"
BASE COUNT          498 a           98 c          211 g          413 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg
//
